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| 1 | A novel strategy for rapid construction of libraries of full-length antibodies highly expressed on mammalian cell surfaces显示文摘一个万用的哺乳动物的显示器系统的开发为有高亲密关系的功能的人的抗体的选择是必要的。这里,我们为能高效地在哺乳动物的房间表面上被表示的全身的抗体图书馆的快速的建设描述了新奇策略。通用向量 pDGB-HC-TM 被插入唯一的序列为兴趣的基因的突然发生在里面和突然发生外面由限制 endonucleases BsmBI, SfiI,和 BstXI 认出了的多重克隆的地点构造。 Cytomegalovirus 倡导者,为在许多哺乳动物的房间的蛋白质的高表示的一个通常使用的倡导者,被用来从导出血小板的生长驾驶插入的抗体基因和一个 transmembrane 领域的表示因素受体在框架被熔化到重链的C终点一致区域到抗体在哺乳动物的房间表面上表示了的锚。用这策略,我们构造了一个全身的人的抗体显示图书馆。DNA 顺序分析和表示分析显示图书馆构造了有 6.58 e 的组合可表现的、可检测的差异 ???汩 l 吗?? | Ye Zhou Zhen-Rui Chen Chang-Zheng Li Wei He Shuwen Liu Shibo Jiang Wen-Li Ma Wanlong Tan Chen Zhou | 2010 | Acta Biochimica et Biophysica Sinica2010，42，8： | 11 |
| 2 | Identification of HBsAg-specific antibodies from a mammalian cell displayed full-length human antibody library of healthy immunized donor显示文摘肝炎 B 免疫球蛋白(HBIG ) 在肝炎 B 的管理是重要的病毒(HBV ) 感染。试图作为 HBIG 的一种选择开发 recombinant monoclonal 抗体,我们报导 HBV 表面抗原(HBsAg ) 的成功的鉴定从在哺乳动物的房间上显示的一个全身的人的抗体图书馆的特定的抗体出现。把一个生来地使免疫的施主的外部血 mononuclear 房间的全部的 RNA 用作模板,抗体全部剧目被放大。联合四方法的结扎和 Flp 调停 recombinase 的集成(Flp 在里面) 系统,我们构造了每个房间在显示了一个种仅仅抗体分子的一个哺乳动物的基于房间的、充分人的、全身的抗体显示图书馆。由屏蔽使用激活荧光的房间排序的房间图书馆(FACS ) ,八个房间克隆显示那房间表面上的 HBsAg 特定的抗体被识别。抗体基因的 DNA 顺序分析揭示了三唯一的抗体。FACS 数据显示了那荧光灯表示(FSE ) 的力量,荧光灯绑定(FSB ) 的力量和相对有约束力的能力(RBA ) 都在他们之中是不同的。这些结果由使用我们的抗体表明了那哺乳动物的显示和屏蔽站台,我们能成功地从一个使免疫的全身的抗体图书馆识别抗原特定的抗体。因此,这个平台为治疗学的抗体的开发是很有用的。 | Chang-Zheng Li Zhong-Kun Liang Zhen-Rui Chen Hai-Bo Lou Ye Zhou Zhe-Huan Zhang Fei Yu Shuwen Liu Yuanping Zhou Shuguang Wu Wenling Zheng Wanlong Tan Shibo Jiang Chen Zhou | 2012 | Cellular & Molecular Immunology2012，9，2： | 7 |
| 3 | Four-way ligation for construction of a mammalian cell-based full-length antibody display library显示文摘唯一的四方法的结扎策略为一个全身的抗体图书馆的快速的建设被开发。哺乳动物的表示向量被构造盒子和序列由唯一的限制酶 BsmBI, BstXI,和 SfiI 认出了的那包含的双哺乳动物的表情。重链的基因的全身的轻链、可变的领域被四方法的结扎在一步插入到向量,并且全身的二原子价的抗体在哺乳动物的房间表面上被显示。用这策略,仅仅 2 个星期被要求成功地构造高质量的、全身的人的抗体图书馆。 | Ivan Zhou Zhe-Huan Zhang Chang-Zheng Li Zhen-Rui Chen Wei He Ye Zhou Shuwen Liu Shuguang Wu Yuanping Zhou Wanlong Tan Shibo Jiang Chen Zhou | 2011 | Acta Biochimica et Biophysica Sinica2011，43，3： | 6 |
| 4 | Hybrid Dirac semimetal-based photodetector with efficient low-energy photon harvesting显示文摘Despite the considerable effort,fast and highly sensitive photodetection is not widely available at the low-photon-energy range(~meV)of the electromagnetic spectrum,owing to the challenging light funneling into small active areas with efficient conversion into an electrical signal.Here,we provide an alternative strategy by efficiently integrating and manipulating at the nanoscale the optoelectronic properties of topological Dirac semimetal PtSe_(2)and its van der Waals heterostructures.Explicitly,we realize strong plasmonic antenna coupling to semimetal states near the skin-depth regime(λ/10^(4)),featuring colossal photoresponse by in-plane symmetry breaking.The observed spontaneous and polarization-sensitive photocurrent are correlated to strong coupling with the nonequilibrium states in PtSe_(2)Dirac semimetal,yielding efficient light absorption in the photon range below 1.24 meV with responsivity exceeding∼0.2 A/W and noise-equivalent power(NEP)less than~38 pW/Hz^(0.5),as well as superb ambient stability.Present results pave the way to efficient engineering of a topological semimetal for high-speed and low-energy photon harvesting in areas such as biomedical imaging,remote sensing or security applications. | Lin Wang Li Han Wanlong Guo Libo Zhang Chenyu Yao Zhiqingzi Chen Yulu Chen Cheng Guo Kaixuan Zhang Chia-Nung Kuo Chin Shan Lue Antonio Politano Huaizhong Xing Mengjie Jiang Xianbin Yu Xiaoshuang Chen Wei Lu | 2022 | Light(Science & Applications)2022，11，3： | 2 |
| 5 | Does diffusion tensor data reflect pathological changes in the spinal cord with chronic injury?显示文摘Magnetic resonance diffusion tensor imaging has been shown to quantitatively measure the early pathological changes in chronic cervical spondylotic myelopathy.In this study,a novel spongy polyurethane material was implanted in the rat C3–5epidural space to establish a rat model of chronic cervical spondylotic myelopathy.Diffusion tensor data were used to predict pathological changes.Results revealed that the fractional anisotropy value gradually decreased at 4,24,and 72 hours and1 week after injury in rat spinal cord,showing a time-dependent manner.Average diffusion coefficient increased at 72 hours and 1 week after implantation.Hematoxylin-eosin staining and Luxol-fast-blue staining exhibited that the number of neurons in the anterior horn of the spinal cord gray matter and the nerve fiber density of the white matter gradually reduced with prolonged compression time.Neuronal loss was most significant at 1 week after injury.Results verified that the fractional anisotropy value and average diffusion coefficient reflected the degree of pathological change in the site of compression in rat models at various time points after chronic spinal cord compression injury,which potentially has a reference value in the early diagnosis of chronic cervical spondylotic myelopathy. | Erjian Lin Houqing Long Guangsheng Li Wanlong Lei | 2013 | Neural Regeneration Research2013，8，36： | 2 |
| 6 | Study on Flow Characteristics of a Turbulent Boundary Layer and Vortex Structure of High Pressure Guide Vanes in SCO_2 Turbines显示文摘In order to further understand the aerothermodynamic performance and flow loss mechanism of SCO_2 turbines, RANS equations and an SST Turbulence Model were chosen for a numerical study on the secondary flow and vortex structure of cascades using the commercial software CFX. The dimensionless vorticity analysis method was used to study the flow characteristics of the logarithmic layer and viscous sublayer in high pressure guide vane cascades. The new vortex structure and formation mechanism of the vortices were given and analyzed. Simulation results indicated that during the motion of the boundary layer in the cascades, the logarithmic layer and viscous sublayer obtain the different rotational direction vorticity, respectively. The endwall logarithmic layer and pressure side leg of the horseshoe votex gradually develop into the passage vortex, with the endwall viscous sublayer gradually developing into the corner viscous sublayer vortex II and the endwall viscous sublayer vortex I. The endwall viscous sublayer that rolled by the passage vortex is encountered with the upstream-side and radial boundary layer of the vane at the suction separation line, forming the suction separation line vortex beside the passage vortex. A pair of radial transition vortices are formed between the wake and the main stream. | HAN Wanlong WANG Yueming FENG Zhenping LI Hongzhi YAO Mingyu ZHANG Yifan | 2019 | Journal of Thermal Science2019，28，3： | 1 |
| 7 | A Novel Therapeutic Vaccine of Mouse GM-CSF Surface Modified MB49 Cells Against Metastatic Bladder Cancer显示文摘 | Xinji Zhang Xiaojun Shi Jinlong Li Zhiming Hu Dong Zhou Jimin Gao Wanlong Tan | 2012 | The Journal of Urology2012，，3： | 1 |
| 8 | Morphological characteristics of the striatal neural pathway by biotinylated dextran amine tracing in rats显示文摘Using neural pathway tracing and immunohistochemical technique, the striato-direct pathway (BDA3 kDa injected into the rat lateral globus pallidus) and striato-indirect pathway (BDA3 kDa injected into the substantia nigra pars reticulata) neurons were specifically labeled, and then subjected to double-labeled immunohistochemistry for mu-OPIOID Receptor (specifically-labeled striatal patch compartment), D1, and D2, respectively. The experimental findings showed that there are no statistically significant differences in the soma diameter and the number of primary dendrites between the striato-direct (substantia nigra pars reticularis) and indirect (globus pallidum externum) neurons labeled retrograde by BDA3 kDa. In addition, these two kinds of projection neurons revealed no obvious coexistence. This evidence indicates that as a highly sensitive neural pathway tracer, BDA could yield reliably and exquisitely detailed labeling of target neurons and synaptic structures. The variance of the morphologic structures and the localization of neurons were not statistically significant between the striato-substantia nigra pars reticularis and the globus pallidum externum projection neurons. Mesencephalic and thalamic neurons correlated with striatal neurons in morphology. Especially the latter which make typical excitatory synaptic contacts with striato-direct and -indirect neurons. Thus, this evidence suggests that thalamic neurons may extensively excite striatal neurons. | Bingbing Liu Shuhua Mu Lisi Ouyang Yaxi Zhu Keyi Li Mali Zhan Zongwei Liu Yu Jia Wanlong Lei | 2011 | Neural Regeneration Research2011，6，25： | 0 |
| 9 | Microstructure and Ultrastructure Changes of Panax ginseng Root Tip that Infected by Cylindrocarpon destructans at Early Stage显示文摘[Objectives] To study the microstructure and ultrastructure changes of P. ginseng root tip infected by C. destructans. [Methods]The paraffin section and superfine slice were used to observe the microstructure and ultrastructure of root tip of P. ginseng,respectively.[Results] Microstructure and ultrastructure observation indicated that,the morphology and structure of epidermal cells of P. ginseng were regular,cells had abundant contents,and organelles were easy to observe in control. However,after infected by C. destructans,the cell walls were thicken seven days post inoculation,particles were deposited in epidemic cells and pith,cell walls were broken,and cells began to disintegrate. Furthermore,morphology of cells was abnormal and contents in cells were decreased when infected by C. destructans. The organelles were break up,and difficult to be distinguished. [Conclusions] Through the results we deduced that,the hypha of C. destructans should firstly invade the epidermal cells of P. ginseng,and then enter into the xylem of P. ginseng root through the intercellular space,which obstructed the transport of water and inorganic salt,and finally led to death of ginseng seedlings. | Yuan GAO Wanlong DING Rong WANG Yong LI | 2018 | Medicinal Plant2018，9，1： | 0 |
| 10 | Dose escalation guided by^(18)F-FDG PET/CT for esophageal cancer显示文摘Objective:To assess the feasibility of dose escalation guided by ^(18)F-deoxyglucose positron emission tomography/computer tomography(^(18)F-FDG PET/CT)for esophageal cancer(EC).Methods and materials:Ten random patients treated with definitive chemoradiotherapy and pre-therapeutic ^(18)FFDG PET/CT were included in this study.Retrospectively,a threshold of 50%of SUVmax was used to define the high FDG uptake region of the GTV(GTVPET).Three intensity-modulated radiation therapy(IMRT)plans were generated,delivering three dose levels to three different planning target volumes(PTVs).50.4 Gy delivered to PTV50.4 was defined on computed tomography(CT)as Plan50.4,63 Gy was delivered to PTV63 was defined as GTV plus a uniform margin of 0.5 cm as Plan63,and 70 Gy delivered to PTV70 was defined as GTVPET plus a 0.5 cm margin as Plan70.A dosimetric comparison was performed based on normal tissue complication probability(NTCP)for the lung and heart.Results:Clinically acceptable dose escalation failed for 2 of 10 patients in Plan63 due to heart dose constraints.One patient failed heart dose constraint for Plan70.Two patients failed spinal cord constraint for Plan63.Three patients failed lung dose constraints for both Plan63 and Plan70,two of which were even not suitable for Plan50.4 for the same reason.NTCP modeling for lung showed increased risk for Plan63 and Plan70 compared to Plan50.4.The difference between Plan63 and Plan70 was insignificant.NTCP modeling for heart showed an increased risk from 6.38%of Plan50.4 to 8.88%of Plan70(P=0.009)or 9.79%of Plan63(P=0.007).The risk of heart mortality was significantly higher for Plan63 than Plan70(P=0.047).Conclusions:Selective boosting of sub-volumes based on ^(18)F-FDG PET/CT is feasible way with a modest increase in the risk of cardiac and lung toxicities. | Bingjie Fan Chengqiang Li Fengchun Mu Wenru Qin Linlin Wang Xindong Sun Chunni Wang Bing Zou Shijiang Wang Wanlong Li Man Hu | 2021 | Radiation Medicine and Protection2021，2，2： | 0 |
| 11 | Global Entrepreneur Summit 2011 Wrapped Up in Beijing显示文摘The year 2011 turns out to be a tough year for global economic recovery with 2012 facing the alternative challenge of political and economic cycle. | You Wanlong, Li Zhen | 2012 | China's Foreign Trade2012，，1： | 0 |
| 12 | National Report on Space Medicine Progress显示文摘The development of Chinese manned space engineering has a great role in promoting the progress of Chinese space medicine. With the accelerated implementation of Chinese Space Station Program, China's space medicine has made fruitful achievements over the past three years. This report summarizes the major progress of Chinese space medicine in terms of basic research and space applications from 2016 to 2018. | LI Yinghui LI Weigang DING Bai WU Dawei LIU Zhaoxia HE Li ZHOU Wanlong XU Zi | 2018 | 空间科学学报2018，38，5： | 0 |
| 13 | New Pearl in the River Delta显示文摘30 years after China's reform and opening-up policy, the Yangtze River Delta has become one of the most prosperous regions in China. In this process, great contributions made by small and medium sized enterprises can not be ignored. In this issue, China's For- eign Trade switched an interview with Mr. Peterson Zhou Lu (Chief. Bobadara of Ijede, Lagos), chairman of board of CD International Group Ltd in Shanghai. As a small and medium sized enterprise leader, Chief.Lu shared his views with us in detail. | Li Zhen You Wanlong | 2008 | China's Foreign Trade2008，，17： | 0 |
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