|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Hepatitis C virus in human B lymphocytes transformed by Epstein-Barr virus in vitro by in situ reverse transcriptase-polymerase chain reaction显示文摘AIM To study persistence and replication ofheltitis C virus (HCV) in patients' peripheralblood mononuclear cells (PBMC) cultured invitro.METHODS Epstein-Barr virus (EBV) was usedto transform the hepatitis C virus from a HCVpositive patient to permanent lymphoblastoidcell lines (LCL). Positive and negative HCV RNAstrands of the cultured cells and growth mediawere detected by reverse transcriptase-polymerase chain reaction ( RT-PCR ) eachmonth. Core and NS5 proteins of HCV werefurther tested using immunohistochemical SPmethod and in situ RT-PCR.RESULTS HCV RNA positive strands wereconsistently detected the cultured cells for oneyear. The negative-strand RNA in LCL cells andthe positive-strand RNA in supernatants wereobserved intermittently. Immunohistochemicalresults medicated expression of HCV NS3 and Cproteins in LCL cytoplasm mostly. The positivesignal of PCR product was dark blue and mainlylocalized to the LCL cytoplasm. The RT-PCRsignal was eliminated by overnight RNasedigestion but not DNase digestion.CONCLUSION HCV may exist and remainfunctional in a cultured cell line for a longperiod. | Ji Lin Cheng Bao Ling Liu Yi Zhang Wen Bin Tong Zheng Yan Bai Fang Feng Institute of Hepatology,Peoples Hospital,Medical Center of Beijing University,Beijing 10(X)44,China | 2001 | World Journal of Gastroenterology2001，7，3： | 11 |
| 2 | Overview to the Hard X-ray Modulation Telescope (Insight-HXMT) Satellite显示文摘As China’s first X-ray astronomical satellite, the Hard X-ray Modulation Telescope (HXMT), which was dubbed as Insight-HXMT after the launch on June 15, 2017, is a wide-band(1-250 ke V) slat-collimator-based X-ray astronomy satellite with the capability of all-sky monitoring in 0.2-3 Me V. It was designed to perform pointing, scanning and gamma-ray burst(GRB)observations and, based on the Direct Demodulation Method (DDM), the image of the scanned sky region can be reconstructed.Here we give an overview of the mission and its progresses, including payload, core sciences, ground calibration/facility, ground segment, data archive, software, in-orbit performance, calibration, background model, observations and some preliminary results. | Shuang-Nan Zhang TiPei Li FangJun Lu LiMing Song YuPeng X u CongZhan Liu Yong Chen XueLei Cao QingCui Bu Zhi Chang Gang Chen Li Chen TianXiang Chen YiBao Chen YuPeng Chen Wei Cui WeiWei Cui JingKang Deng YongWei Dong Yuan Yuan Du MinXue Fu GuanHua Gao He Gao Min Gao MingYu Ge YuDong Gu Ju Guan Can Gungor ChengCheng Guo DaWei Han Wei Hu Yue Huang Jia Huo ShuMei Jia LuHua Jiang WeiChun Jiang Jing Jin YongJie Jin Bing Li ChengKui Li Gang Li MaoShun Li Wei Li Xian Li XiaoBo Li XuFang Li YanGuo Li ZiJian Li ZhengWei Li XiaoHua Liang JinYuan Liao GuoQing Liu HongWei Liu ShaoZhen Liu XiaoJing Liu Yuan Liu YiNong Liu Bo Lu XueFeng Lu Tao Luo Xiang Ma Bin Meng Yi Nang JianYin Nie Ge Ou JinLu Qu Na Sai RenCheng Shang GuoHong Shen Liang Sun Ying Tan Lian Tao YouLi Tuo Chen Wang ChunQin Wang GuoFeng Wang HuanYu Wang Juan Wang WenShuai Wang YuSa Wang XiangYang Wen BaiYang Wu BoBing Wu Mei Wu GuangCheng Xiao ShaoLin Xiong LinLi Yan JiaWei Yang Sheng Yang YanJi Yang QiBin Yi Bin Yuan AiMei Zhang ChunLei Zhang ChengMo Zhang Fan Zhang HongMei Zhang Juan Zhang Qiang Zhang ShenYi Zhangs Shu Zhang Tong Zhang WanChang Zhang Wei Zhang WenZhao Zhang Yi Zhang YiFei Zhang YongJie Zhang Yue Zhang Zhao Zhang Zhi Zhang ZiLiang Zhang HaiSheng Zhao XiaoFan Zhao ShiJie Zheng JianFeng Zhou YuXuan Zhu Yue Zhu RenLin Zhuang | 2020 | Science China(Physics,Mechanics & Astronomy)2020，63，4： | 2 |
| 3 | Development and validation of an all-cause mortality risk score in type 2 diabetes显示文摘 | Yang X So W Y Tong P C | 2008 | Arch Intern Med2008，168，5： | 1 |
| 4 | Metal-organic molecular architectures with 2,2′-bipyridyl-like and carboxylate ligands显示文摘 | Ye B H Tong M L Chen X M | 2005 | Coord Chem Rev2005，249，： | 1 |
| 5 | Precise time integration for linear two-point boundary value problems显示文摘 | Chen B S Tong L Y Gu Y X | 2006 | Appl Math Comput2006，175，： | 1 |
| 6 | Inverse Molecular WeightDependence of Cloud Points for Aqueous Poly (Nisopropylacrylamide) Solutions显示文摘 | TONG Z ZENG F ZHENG X | 1999 | Macromolecules1999，32，13： | 1 |
| 7 | Hybird wavelet - Kalman fil- ter method for forecasting显示文摘 | Tong X Girgis A Makram E | 2000 | Electric Power Systems Research2000，54，1： | 1 |
| 8 | Vascular oxidative stress: the com- mon link in hyper- tensive and diabetic vascular disease 显示文摘 | Cohen RA Tong X | 2010 | J Cardiovasc Pharmaeol2010，55，4： | 1 |
| 9 | J Mater Sci显示文摘 | Tong X Okano T lseki T Yano T | 1995 | 30:3087-30901995，30，： | 1 |
| 10 | Characterization of a Nano- crystalline NiTiHf High Temperature Shape Memory Alloy Thin Film显示文摘 | Tong Y X Liu Y Miao J M | 2005 | Scripta Materialia2005，52，： | 1 |
| 11 | One-pot synthesis of brush diblock copolymers through simultaneous ATRP and click coupling 显示文摘 | Han D H Tong X Zhao Y | 2011 | Macromolecules2011，44，13： | 1 |
| 12 | Highly pathogenic porcine reproductive and respiratory syndrome, China 显示文摘 | Tong G Z Zhou Y J Hao X F | 2007 | Emerg Infect Dis2007，13，9： | 1 |
| 13 | Adaptive fuzzy approach to control unified chaotic systems显示文摘 | Chen B Liu X P Tong S C | 2007 | Chaos Solitons and Fractals2007，34，4： | 1 |
| 14 | Adaptive fuzzy output tracking control of MIMO nonlinear uncertain systems 显示文摘 | CHEN B LIU X TONG S | 2007 | IEEE Transactions on Fuzzy Systems2007，15，2： | 1 |
| 15 | The adhesion of bionic non-smooth characteristics on sample surfaces against parts 显示文摘 | Zhou H Shah H Y Tong X | 2006 | Materials Science & Engineering2006，417，12： | 1 |
| 16 | Surface modification of puretitanium treated with B4C at high temperature显示文摘 | He L J Zhang X N Tong C D | 2006 | Surface &Coatings Technology2006，200，9： | 1 |
| 17 | Effect of polysavone (alfalfa extract) on abdominal fat deposition and immunity in broiler chickens显示文摘 | Dong X F Gao W W Tong J M | 2007 | Poultry Science2007，86，9： | 1 |
| 18 | Hypoxia-induced reactive oxygen species downregulate ETB receptor-mediated contraction of rat pulmonary arteries显示文摘 | Wang X Tong M Chinta S | | 0，，03： | 1 |
| 19 | A novel nanostructure with hexagonal-prism pores fabricated under vacuum circumstance显示文摘 | Tong H Cheng Z Lv X Song Y D.Yu | | 0，，： | 1 |
| 20 | Highly pathogenic porcine reproductive and respiratory syndrome,China显示文摘 | Tong G Z Zhou Y J Hao X F | | 0，，09： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员