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| 1 | The Reference Genome of Tea Plant and Resequencing of 81 Diverse Accessions Provide Insights into Its Genome Evolution and Adaptation显示文摘Tea plant is an important economic crop,which is used to produce the world's oldest and most widely consumed tea beverages.Here,we present a high-quality reference genome assembly of the tea plant(Camellia sinensis var.sinensis)consisting of 15 pseudo-chromosomes.LTR retrotransposons(LTR-RTs)account for 70.38%of the genome,and we present evidence that LTR-RTS play critical roles in genome size expansion and the transcriptional diversification of tea plant genes through preferential insertion in promoter regions and introns.Genes,particularly those coding for terpene biosynthesis pro-teins,associated with tea aroma and stress resistance were significantly amplified through recent tandem duplications and exist as gene clusters in tea plant genome.Phylogenetic analysis of the sequences of 81 tea plant accessions with diverse origins revealed three well-differentiated tea plant populations,support-ing the proposition for the southwest origin of the Chinese cultivated tea plant and its later spread to western Asia through introduction.Domestication and modern breeding left significant signatures on hundreds of genes in the tea plant genome,particularly those associated with tea quality and stress resis-tance.The genomic sequences of the reported reference and resequenced tea plant accessions provide valuable resources for future functional genomics study and molecular breeding of improved cul-tivars of tea plants. | Enhua Xia Wei Tong Yan Hou Yanlin An Linbo Chen Qiong Wu Yunlong Liu Jie Yu Fangdong Li Ruopei Li Penghui Li Huijuan Zhao Ruoheng Ge Jin Huang Ali Inayat Mallano Yanrui Zhang Shengrui Liu Weiwei Deng Chuankui Song Zhaoliang Zhang Jian Zhao Shu Wei Zhengzhu Zhang Tao Xia Chaoling Wei Xiaochun Wan | 2020 | Molecular Plant2020，13，7： | 51 |
| 2 | A novel gene delivery system targeting cells expressing VEGF receptors显示文摘Two ligand oligopeptides GV1 and GV2 were designed according to the putative binding region of VEGF to its receptors. GV1, GV2 and endosome releasing oligopeptide HA20 were conjugated with poly-L-lysine or protamine and the resulting conjugates could interact with DNA in a noncovalent bond to form a complex. Using pSV2-β-galactosidase as a reporter gene, it has been demonstrated that exogenous gene was transferred into bovine aortic arch-derived endothelial cells (ABAE) andhuman malignant melanoma cell lines (A375) in vitro. In vivo experiments, exogenous gene was transferred into tumor vascular endothelial cells and tumor cells of subcutaneously transplanted human colon cancer LOVO, human malignant melanoma A375 and human hepatoma graft in nude mice. This system could also target gene to intrahepatically transplanted human hepatoma injected via portal vein in nude mice. These results are correlated with theGene delivery system targeting VEGF receptors relevant receptors (flt-1, flk-1/KDR) expression on the targeted cells and tissues. | LI JUN MIN JUN SONG HAN YI HUANG PEI KUN TIAN SHU MIN QU MIN YAO HUI QIU JIANG DA FANG WAN JING CHU LUO CHENG XIAO GU JIAN REN GU( National Labomtory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032,China)(National Laboratory of | 1999 | Cell Research1999，9，1： | 22 |
| 3 | Functional CT for assessment of early vascular physiology in liver tumors显示文摘BACKGROUND:CT perfusion has been reported to have great advantages in detecting hepatic diseases.However, currently there are no studies showing the potential value of multi-slice CT perfusion with the deconvolution model method in diagnosing early hemodynamic changes caused by liver tumors.This study was undertaken to determine if early hemodynamic changes caused by liver tumors can be depicted with the perfusion imaging of multi-slice CT. METHODS:Ten New Zealand white rabbits before and after VX2 liver tumor inoculation served as the experimental animals.Ten normal rabbits served as controls.All underwent multi-slice CT perfusion for the measurement of hepatic blood flow(HBF),hepatic blood volume(HBV), mean transit time(MTT),permeability of capillary vessel surface(PS)and hepatic artery index(HAI). RESULTS:With the exception of MTT,which decreased significantly at the tumor periphery,HBF,HBV,PS and HAI increased significantly compared with the surrounding normal tissue.All these changes occurred at days 5-9 after tumor inoculation.Statistically significant changes in these values were detected with tumor growth. CONCLUSIONS:The hemodynamic changes in the liver caused by rabbit VX2 liver tumor can be detected after tumor inoculation,and functional CT can evaluate the physiological characteristics of early tumor angiogenesis. | Jiang, Hui-Jie Zhang, Zai-Ren Shen, Bao-Zhong Wan, Yong Guo, Hong Shu, Sheng-Jie | 2008 | Hepatobiliary & Pancreatic Diseases International2008，7，5： | 15 |
| 4 | The Predictive Value of Baseline HBs Ag Level and Early Response for HBs Ag Loss in Patients with HBe Ag-positive Chronic Hepatitis B during Pegylated Interferon Alpha-2a Treatment显示文摘Objective To explore the predictive value of baseline HBsAg level and early response for HBsAg loss in patients with HBeAg-positive chronic hepatitis B during pegylated interferon alpha-2a treatment. Methods A total of 121 patients with HBeAg-positive chronic hepatitis B who achieved HBsAg loss were enrolled; all patients were treated with PEG-IFNα-2a 180 μg/week. Serum HBV DNA and serological indicators(HBsAg, anti-HBs, HBeAg, and anti-HBe) were determined before and every 3 months during treatment. Results The median treatment time for HBsAg loss was 84 weeks(7-273 weeks), and 74.38%(90 cases) of the patients needed extended treatment(> 48 weeks). The correlation between baseline HBsAg levels and the treatment time of HBsAg loss was significant(B = 14.465, t = 2.342, P = 0.021). Baseline HBsAg levels together with the decline range of HBsAg at 24 weeks significantly correlated with the treatment time of HBsAg loss(B = 29.862, t = 4.890, P = 0.000 and B = 27.993, t = 27.993, P = 0.005). Conclusion Baseline HBsAg levels and extended therapy are critical steps toward HBsAg loss. Baseline HBsAg levels together with early response determined the treatment time of HBsAg loss in patients with HBeAg-positive chronic hepatitis B during pegylated interferon alpha-2a treatment. | LI Ming Hui ZHANG Lu QU Xiao Jing LU Yao SHEN Ge LI Zhen Zhen WU Shu Ling LIU Ru Yu CHANG Min HU Lei Ping HUA Wen Hao SONG Shu Jing WAN Gang XIE Yao | 2017 | Biomedical and Environmental Sciences2017，30，3： | 14 |
| 5 | Identification and Typing of Respiratory Adenoviruses in Guangzhou, Southern China Using a Rapid and Simple Method显示文摘Human adenoviruses (HAdVs), especially HAdV-B3, -E4 and -B7, are associated with Acute Respiratory Disease in Chinese children, and occasionally in adults. In order to establish and document the profiles of the respiratory adenovirus pathogen among children in Guangzhou, Southern China, a rapid, simple and practical method for identification and typing of respiratory adenoviruses was developed and evaluated. One pair of universal PCR primers was designed according to the conserved region of the hexon gene, which can detect not only HAdV-B3, -E4 and -B7, but also HAdV-B14, -F40 and -F41, with a specific 300bp PCR product. Three pairs of type-specific PCR primers were also designed according to the hypervariable regions of the hexon gene to type HAdV-B3, -E4 and -B7 by three independent PCR reactions, making it easy to optimize the PCR conditions. By using this method, one hundred throat swab specimens collected during Oct 2010 to Dec 2011 and suspected of being positive for adenoviral infection were identified and typed for adenoviruses. Of these samples, fifty-five were adenovirus-positive. The most common HAdV type was HAdV-B3, identified in 92.7% of samples, which is not only consistent with the data reported in 2004-2006, but also consistent with the recent report in Hangzhou, eastern China, indicating that HAdV-B3 has been circulating in Guangzhou, and maybe in eastern China, for many years. The method for the respiratory adenovirus identification and typing we developed is rapid, simple and practical, which has a potential in the real-time surveillance of circulating adenovirus strains and also to provide etiological evidence for the adenovirus-relative disease control and prevention in China. | Guiyuan Han Hongling Niu Suhui Zhao Bing Zhu Changbing Wang Yungang Liu Mingjie Zhang Shu Yang Feitong Liu Chengsong Wan Qiwei Zhang | 2013 | Virologica Sinica2013，28，2： | 10 |
| 6 | A cross-sectional study on HGV infection in a rural population显示文摘AbstractAIMTodeterminetheepidemiologicalcharacteristicsandclinicalsignificanceofHGVinfection,andtocomparewithHBVandHCVinfecti... | LING Bin Hua 1, ZHUANG Hui 1, CUI Yi Hui 1, AN Wen Feng 1, LI Zhi Jie 1, WANG Shu Ping 2 and ZHU Wan Fu 2 | 1998 | World Journal of Gastroenterology1998，4，6： | 10 |
| 7 | Fourth C hinese National Consensus Report on the management of H elicobacter pylori infection显示文摘 | Wen Zhong Liu Yong Xie Hong Cheng Nong Hua Lu Fu Lian Hu Wan Dai Zhang Li Ya Zhou Ye Chen Zhi Rong Zeng Chong Wen Wang Shu Dong Xiao Guo Zong Pan Pin Jin Hu | 2013 | Journal of Digestive Diseases2013，，5： | 9 |
| 8 | A Novel Thixotropic Phenomenon——Complex Thixotropic Behavior显示文摘ANovelThixotropicPhenomenon——ComplexThixotropicBehavior*Towhomcorrespondenceshouldbeaddresed.HOUWan-guo*,SUNDe-jun,HANShu-hua... | HOU Wan guo, SUN De jun, HAN Shu hua, ZHANG Chun guang and WANG Guo ting (Institute of Colloid and Interface Chemistry, Shandong University , Jinan, 250100) | 1997 | Chemical Research in Chinese Universities1997，13，1： | 8 |
| 9 | Genome analysis and signature discovery for diving and sensory properties of the endangered Chinese alligator显示文摘Crocodilians 正在潜水能为时间的长时期在水下面屏息并且是有优秀感觉能力的黄昏的动物的爬行动物。他们包括鸟的一个姐妹系并且没有性染色体。这里,我们报导危及的中国短吻鳄(短吻鳄 sinensis ) 的染色体顺序并且描述它的唯一的特征。下一代的定序产生了未加工的顺序的 314 Gb,产出 2.3 Gb 的一种染色体尺寸。22 200 基因的一个总数用 de novo 在短吻鳄 sinensis 被预言,基于相同、基于 RNA 的联合模型。长潜水的行为的基因基础包括酸式碳酸盐绑定血红素基因,磷酸盐绑定和特殊酸式碳酸盐绑定氧搬运的常规的 co-functioning,和断然选择的精力的复制新陈代谢,铵酸式碳酸盐排泄和心肌收缩。进一步,我们阐明了柔韧的短吻鳄 sinensis 感觉系统包括一个显著地扩展的嗅觉的受体全部剧目,很快演变神经相关的细胞的部件和视觉感觉,和夜里的积极选择视觉相关的视蛋白和健全联系察觉的 otopetrin。我们也为抗菌剂肽的天生的免疫和表示为分成三部分的包含主题的 C 类型 lectin 和 butyrophilin 基因的适应免疫以及扩大与系特定的抗原演讲基因的一个可观的数字发现了一个开发得好的免疫系统。在 situ 杂交的 Multifluorescence 显示出那个短吻鳄染色体 3,它编码 DMRT1,与鸡染色体 Z 一起展出重要 synteny。最后,人口历史分析百万年以前显示了人口混合 0.60-1.05,当 Qinghai 西藏的高原被高举时。 | Qiu-Hong Wan Sheng-Kai Pan Li Hu Ying Zhu Peng-Wei Xu Jin-Quan Xia Hui Chen Gen-Yun He Jing He Xiao-Wei Ni Hao-Long Hou Sheng-Guang Liao Hai-Qiong Yang Ying Chen Shu-Kun Gao Yun-Fa Ge Chang-Chang Cao Peng-Fei Li Li-Ming Fang Li Liao Shu Zhang Meng-Zhen Wang Wei Dong Sheng-Guo Fang | 2013 | Cell Research2013，23，9： | 8 |
| 10 | Association of Overlapped and Un-overlapped Comorbidities with COVID-19 Severity and Treatment Outcomes: A Retrospective Cohort Study from Nine Provinces in China显示文摘Objective Several COVID-19 patients have overlapping comorbidities. The independent role of each component contributing to the risk of COVID-19 is unknown, and how some non-cardiometabolic comorbidities affect the risk of COVID-19 remains unclear.Methods A retrospective follow-up design was adopted. A total of 1,160 laboratory-confirmed patients were enrolled from nine provinces in China. Data on comorbidities were obtained from the patients’ medical records. Multivariable logistic regression models were used to estimate the odds ratio(OR) and 95% confidence interval(95% CI) of the associations between comorbidities(cardiometabolic or non-cardiometabolic diseases), clinical severity, and treatment outcomes of COVID-19.Results Overall, 158(13.6%) patients were diagnosed with severe illness and 32(2.7%) had unfavorable outcomes. Hypertension(2.87, 1.30–6.32), type 2 diabetes(T2 DM)(3.57, 2.32–5.49),cardiovascular disease(CVD)(3.78, 1.81–7.89), fatty liver disease(7.53, 1.96–28.96), hyperlipidemia(2.15, 1.26–3.67), other lung diseases(6.00, 3.01–11.96), and electrolyte imbalance(10.40, 3.00–26.10)were independently linked to increased odds of being severely ill. T2 DM(6.07, 2.89–12.75), CVD(8.47,6.03–11.89), and electrolyte imbalance(19.44, 11.47–32.96) were also strong predictors of unfavorable outcomes. Women with comorbidities were more likely to have severe disease on admission(5.46,3.25–9.19), while men with comorbidities were more likely to have unfavorable treatment outcomes(6.58, 1.46–29.64) within two weeks.Conclusion Besides hypertension, diabetes, and CVD, fatty liver disease, hyperlipidemia, other lung diseases, and electrolyte imbalance were independent risk factors for COVID-19 severity and poor treatment outcome. Women with comorbidities were more likely to have severe disease, while men with comorbidities were more likely to have unfavorable treatment outcomes. | MA Yan ZHU Dong Shan CHEN Ren Bo SHI Nan Nan LIU Si Hong FAN Yi Pin WU Gui Hui YANG Pu Ye BAI Jiang Feng CHEN Hong CHEN Li Ying FENG Qiao GUO Tuan Mao HOU Yong HU Gui Fen HU Xiao Mei HU Yun Hong HUANG Jin HUANG Qiu Hua HUANG Shao Zhen JI Liang JIN Hai Hao LEI Xiao LI Chun Yan LI Min Qing LI Qun Tang LI Xian Yong LIU Hong De LIU Jin Ping LIU Zhang MA Yu Ting MAO Ya MO Liu Fen NA Hui WANG Jing Wei SONG Fang Li SUN Sheng WANG Dong Ting WANG Ming Xuan WANG Xiao Yan WANG Yin Zhen WANG Yu Dong WU Wei WU Lan Ping XIAO Yan Hua XIE Hai Jun XU Hong Ming XU Shou Fang XUE Rui Xia YANG Chun YANG Kai Jun YUAN Sheng Li ZHANG Gong Qi ZHANG Jin Bo ZHANG Lin Song ZHAO Shu Sen ZHAO Wan Ying ZHENG Kai ZHOU Ying Chun ZHU Jun Teng ZHU Tian Qing ZHANG Hua Min WANG Yan Ping WANG Yong Yan 无 | 2020 | Biomedical and Environmental Sciences2020，33，12： | 8 |
| 11 | Prognostic Impact of Fasting Plasma Glucose on Mortality and Re-Hospitalization in Patients with Acute Heart Failure显示文摘 | Yu-Yang Chen Yuan Chen Shu-Min Liang Zi-Zhuo Su Xiao-Rong Shu Hai-Feng Zhang Siu-Hin Wan Jing-Feng Wang Shuang-Lun Xie | 2018 | Chinese Medical Journal2018，，17： | 7 |
| 12 | A mechanically tuned Kerr comb in a dispersion-engineered silica microbubble resonator显示文摘This paper discusses the developement and investigation of a silica microbubble resonator(MBR) that is optimized to cancel mode dispersion with material dispersion, at a wavelength of approximately 1550 nm and maintain a quality factor of an optical mode as large as 5.4 × 10~7. Benefitting from the near-zero dispersion and high quality factor, a primary optical comb is generated in the MBR using cascaded four-wave mixing processes, which span over 300 nm with several tens of teeth. Furthermore, the frequency comb could be gradually tuned by mechanically stretching the MBR. This tunable Kerr comb has multiple potential applications in precision measurements and sensing applications, such as molecular spectroscopy and ranging. | FangJie Shu PeiJi Zhang YanJun Qian ZhengYu Wang Shuai Wan Chang Ling Zou GuangCan Guo ChunHua Dong | 2020 | Science China(Physics,Mechanics & Astronomy)2020，63，5： | 5 |
| 13 | Vacancies and Antisites in B2 FeAl and DO_3 Fe_3Al with a Modified Analytic EAM Model显示文摘A simple modified analytic EAM model for bcc Fe and fcc Al was used to calculate the lattice constant and elastic constants of B2 FeAl and DO3 Fe3Al alloys. The formation energies of vacancy and antisite were also calculated. The present calculations are in agreement with the experimental data and the theoretical results obtained by other authors. | Xiaolin SHU, Wan gyu HU, Hanning XIAO and Huiqiu DENG College of Materials Science and Engineering, Hunan University, Changsha 410082, China Ban gwei ZHANG International Centre of Materials Physics, Chinese Academy of Sciences, S | 2001 | Journal of Materials Science & Technology2001，17，6： | 4 |
| 14 | SARS-CoV-2 Omicron variant:recent progress and future perspectives显示文摘Since the outbreak of the coronavirus disease 2019(COVID-19)pandemic,there have been a few variants of the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),one of which is the Omicron variant(B.1.1.529).The Omicron variant is the most mutated SARS-CoV-2 variant,and its high transmissibility and immune evasion ability have raised global concerns.Owing to its enhanced transmissibility,Omicron has rapidly replaced Delta as the dominant variant in several regions.However,recent studies have shown that the Omicron variant exhibits reduced pathogenicity due to altered cell tropism.In addition. | Yao Fan Xiang Li Lei Zhang Shu Wan Long Zhang Fangfang Zhou | 2022 | Signal Transduction and Targeted Therapy2022，7，5： | 4 |
| 15 | Factors influencing teamwork and collaboration within a tertiary medical center显示文摘AIM: To understand how work climate and related factors influence teamwork and collaboration in a large medical center. METHODS: A survey of 3462 employees was conducted to generate responses to Sexton's Safety Attitudes Questionnaire(SAQ) to assess perceptions of work environment via a series of five-point, Likert-scaled questions. Path analysis was performed, using teamwork(TW) and collaboration(CO) as endogenous variables. The exogenous variables are effective communication(EC), safety culture(SC), job satisfaction(JS), work pressure(PR), and work climate(WC). The measure-ment instruments for the variables or summated subscales are presented. Reliability of each sub-scale are calculated. Alpha Cronbach coefficients are relatively strong: TW(0.81), CO(0.76), EC(0.70), SC(0.83), JS(0.91), WP(0.85), and WC(0.78). Confirmatory factor analysis was performed for each of these constructs. RESULTS: Path analysis enables to identify statistically significant predictors of two endogenous variables, teamwork and intra-organizational collaboration. Significant amounts of variance in perceived teamwork(R2 = 0.59) and in collaboration(R2 = 0.75) are accounted for by the predictor variables. In the initial model, safety culture is the most important predictor of perceived teamwork, with a β weight of 0.51, and work climate is the most significant predictor of collaboration, with a β weight of 0.84. After eliminating statistically insignificant causal paths and allowing correlated predictors1, the revised model shows that work climate is the only predictor positively influencing both teamwork(β = 0.26) and collaboration(β = 0.88). A relatively weak positive(β = 0.14) but statistically significant relationship exists between teamwork and collaboration when the effects of other predictors are simultaneously controlled.CONCLUSION: Hospital executives who are interested in improving collaboration should assess the work climate to ensure that employees are operating in a setting conducive to intra-organizational collaboration. | Shu Feng Chien Thomas TH Wan Yu-Chih Chen | 2012 | World Journal of Methodology2012，2，2： | 4 |
| 16 | Overview of the 2022 WHO Classification of Pituitary Adenomas/Pituitary Neuroendocrine Tumors:Clinical Practices,Controversies,and Perspectives显示文摘The latest edition of the WHO classification of the central nervous system was published in 2021.This review summarizes the major revisions to the classification of anterior pituitary tumors.The most important revision involves preferring the terminology of pituitary neuroendocrine tumor(PitNET),even though the terminology of pituitary adenoma(PA)still can be used according to this WHO classification compared to the previous one.Moreover,immunohistochemistry(IHC)examination of pituitary-specific transcription factors(TFs),including PIT1,TPIT,SF-1,GATA2/3,and ERα,is endorsed to determine the tumor cell lineage and to facilitate the classification of PitNET/PA subgroups.However,TF-negative IHC staining indicates PitNET/PA with no distinct cell lineages,which includes unclassified plurihormonal(PH)tumors and null cell(NC)tumors in this edition.The new WHO classification of PitNET/PA has incorporated tremendous advances in the understanding of the cytogenesis and pathogenesis of pituitary tumors.However,due to the shortcomings of the technology used in the diagnosis of PitNET/PA and the limited understanding of the tumorigenesis of PitNET/PA,the application of this new classification system in practice should be further evaluated and validated.Besides providing information for deciding the follow-up plans and adjunctive treatment after surgery,this classification system offers no additional help for neurosurgeons in clinical practice,especially in determining the treatment strategies.Therefore,it is necessary for neurosurgeons to establish a comprehensive pituitary classification system for PitNET/PA that incorporates neuroimaging grading data or direct observation of invasiveness during operation or the predictor of prognosis,as well as pathological diagnosis,thereby distinguishing the invasiveness of the tumor and facilitating neurosurgeons to decide on the treatment strategies and follow-up plans as well as adjunctive treatment after surgery. | Xue-yan WAN Juan CHEN Jun-wen WANG Yan-chao LIU Kai SHU Ting LEI | 2022 | Current Medical Science2022，42，6： | 4 |
| 17 | Construction of three-dimensional hierarchical porous nitrogen-doped re duce d graphene oxide/hollow cobalt ferrite composite aerogels toward highly efficient electromagnetic wave absorption显示文摘The development of graphene-based composites with low density,robust absorption,wide bandwidth and thin thickness remained a great challenge in the field of electromagnetic(EM)absorption.In this work,nitrogen-doped reduced graphene oxide/hollow cobalt ferrite(NRGO/hollow CoFe_(2)O_(4))composite aerogels were constructed by a solvothermal and hydrothermal two-step route.Results demonstrated that the as-fabricated composite aerogels had the ultralow density and a unique three-dimensional(3D)network structure,and lots of hollow CoFe_(2)O_(4)microspheres were almost homogeneously distributed on the wrinkled surfaces of lamellar NRGO.Moreover,superior EM absorbing capacity could be achieved by modulating the ferrite structure,addition amounts of hollow CoFe_(2)O_(4)and thicknesses.It was noteworthy that the NRGO/hollow CoFe_(2)O_(4)composite aerogel with the addition amount of ferrite of 15.0 mg pos-sessed the minimum reflection loss of-44.7 dB and maximum absorption bandwidth of 5.2 GHz(from 12.6 to 17.8 GHz)at a very thin thickness of 1.8 mm and filling ratio of 15.0 wt.%.Furthermore,the possible EM attenuation mechanism had been proposed.The results of this work would be helpful for developing RGO-based 3D composites as lightweight,thin and highly efficient EM wave absorbers. | Jing Xu Ruiwen Shu Zongli Wan Jianjun Shi | 2023 | Journal of Materials Science & Technology2023，，1： | 4 |
| 18 | Late Mesozoic Thermotectonic Evolution of the Jueluotage Range,Eastern Xinjiang,Northwest China:Evidence from Apatite Fission Track Data显示文摘摘要:尽管许多作者强调了变丑的新生代历史,发掘并且在与印度亚洲碰撞有关的 Tianshan 区域冷却,几乎不对压缩的中生代历史被知道并且在 Tianshan 以内高举。以便关于中生代发掘历史和在东方 Tianshan 冷却的进程获得信息,磷灰石上的分裂磁道方法被使用。采样在 Jueluotage 范围被做。三件样品(Z001-Z003 ) 在 Yandong pluton 的地上凿穿 ZK6301 从花岗石被拿;从比是的 pluton 年龄年轻得多的 97.0 ~ 87.6 妈的年龄范围在 334 点由 U-Pb 锆石标明日期 ????????? Y ?? 霈吗?? | ZHU Wenbin WAN Jinglin SHU Liangshu ZHANG Zhiyong SU Jinbao SUN Yah GUO Jichun ZHANG Xueyun | 2008 | Acta Geologica Sinica(English Edition)2008，82，2： | 4 |
| 19 | In Silico Analysis and Feeding Assays of Some Genes in the Early Steps of Terpenoid Biosynthetic Pathway in Camellia Sinensis显示文摘Terpenoids play important roles in productivity and product quality in tea(Camellia sinensis),not only because volatile terpenoids contribute significantly to the aroma of teas,but terpenoid-derived light harvesting pigments and phytohormones such as strigolactones implement photosynthesis and branching in plants.All terpenoids are derived from isopentenyl diphosphate(IPP),dimethylallyl diphosphate(DMAPP) produced by methyl-erythritol-phosphate(MEP) and mevalonate(MVA) pathways,the early steps of isoprenoid biosynthesis.For the purpose to determine key genes in the MEP and MVA pathways in C.sinensis,five genes with complete coding sequence and three with partial sequence were obtained based on previously attained transcriptome data and polymerase chain reaction approach in this study.In silico analyses suggested that the five genes with full coding sequence individually encoded acetyl-CoA C-acetyltransferase(CsAACT),3-hydroxy-3-methylglutaryl-CoA synthase(CsHMGS),4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase(CsCMK),1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase(CsHDS),and farnesyl pyrophosphate synthase(CsFPS).Additionally,studies on metabolite-mediated gene expression were performed using tea cell suspensions and leaf-disc cultures.Our data showed that the addition of acetyl-CoA increased the transcript level of CsAACT and CsHMGS by 1.5- and 3-fold,respectively.The addition of deoxy-D-xylulose 5-phosphate(DXP) induced the expression of CsCMK and CsHDS by 2.3- and 1.5-fold,respectively.The CsFPS expression was approximately 2-fold higher when treated with DXP,IPP or DMAPP than the untreated control,but this induction was not observed with the acetyl-CoA treatment.Our studies indicated that expression of CsAACT,CsHMGS,CsCMK,CsHDS,and CsFPS were metabolite-mediated in a different extent. | Wei Xiang Yan Xu Fu-Min Wang Li-Ping Gao Ming-Jun Gao Zheng-Zhu Zhang Xiao-Chun Wan Shu Wei | 2013 | 茶叶2013，39，4： | 4 |
| 20 | Comparison of the efficacy of triple versus quadruple therapy on the eradication of Helicobacter pylori and antibiotic resistance显示文摘 | QingZHENG Wan JunCHEN HongLU Qin JuanSUN Shu DongXIAO | 2010 | Journal of Digestive Diseases2010，，5： | 3 |
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