|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Structure and deformation around the Gyirong basin, north Himalaya, and onset of the south Tibetan detachment system显示文摘Gyirong basin and its adjacent area are located at a special position in the Himalayan orogen, where the south Tibetan detachment system (STDS) and N-S trending rift converged. The north Himalayan orogen here can be divided into five petrologic-tectonic units successively from south to north: 1) the Greater Himalayan crystalline complex (GHC); 2) the STDS shear zone; 3) the Tethyan Himalayan sedimentary sequence (THS); 4) the late Cenozoic sedimentary basins, such as Gyirong and Oma basins; and 5) the Malashan gneiss dome. Structural studies show that this area experienced four stages of deformation: 1) the earlier south-directed thrusting, preserved both in the GHC and THS; 2) top-down-to-north slip along the STDS, normal faults related to this slip formed the early controlling structures of the Cenozoic basins,and the tilted pattern of the blocks between the basins indicated a north-directed slip; 3) east-west extension, the resultant N-S trending normal fault formed the eastern boundary of the basins; and 4) late gravitational collapse. Zircon SHRIMP U-Pb dating on the syn-deformational (leuco-) granite along the STDS indicates that the major activity of the STDS occurred at ca. 26 Ma, but its onset may have begun as early as ca. 36 Ma. | YANG XiongYing ZHANG JinJiang QI GuoWei WANG DeChao GUO Lei LI PengYuan LIU Jiang | 2009 | Science China Earth Sciences2009，52，8： | 33 |
| 2 | In vitro Anthocyanin Induction and Metabolite Analysis in Malus spectabilis Leaves Under Low Nitrogen Conditions显示文摘Anthocyanins are the most widely produced secondary metabolites in plants,and they play an important role in plant growth and reproduction.The nitrogen source is an important factor affecting anthocyanin production,but the nitrogen concentrations on metabolism and the underlying genetic basis remain unclear.In this study,in vitro anthocyanin induction was conducted on Malus spectabilis.The leaf explants were cultivated in media containing different nitrogen concentrations.The results suggested that when the nitrogen contents decreased in limit,the color of leaf explants turned from green to red,and increased anthocyanin accumulation led to a change in phenotype.Furthermore,the content of other flavonoids,such as dihydroquercetin,epicatechin,and catechin,increased under low nitrogen conditions.The transcription levels of the general flavonoid pathway genes,from phenylalanine ammonia lyase(PAL)to anthocyanidin synthase(ANS),were associated with the concentration of corresponding flavonoid compounds and phenotype changes.In particular,the expression level of ANS increased substantially under a low nitrogen treatment,which was significantly and positively correlated with the anthocyanin levels(R2=0.72,P<0.05).The increased expression patterns of anthocyanin pathway genes were similar to that of the transcription factor MYB10.We further verified MYB10 played an important role in the anthocyanin pathway in leaves of Malus spectabilis.These results suggested that we can improve the desirable ornamental plant phenotypes by controlling nitrogen content.This process may offer clues to further development of new agricultural practices. | Jiaxin Meng Yan Gao Meiling Han Pengyuan Liu Chen Yang Ting Shen Houhua Li | 2020 | Horticultural Plant Journal2020，6，5： | 13 |
| 3 | The hepatitis B virus-associated tumor microenvironment in hepatocellular carcinoma显示文摘In contrast to a majority of cancer types, the initiation of hepatocellular carcinoma(HCC) is intimately associated with a chronically diseased liver tissue, with one of the most prevalent etiological factors being hepatitis B virus(HBV). Transformation of the liver in HBV-associated HCC oten follows from or accompanies long-term symptoms of chronic hepatitis, inlammation and cirrhosis, and viral load is a strong predictor for both incidence and progression of HCC. Besides aiding in transformation, HBV plays a crucial role in modulating the accumulation and activation of both cellular components of the microenvironment,such as immune cells and ibroblasts, and non-cellular components of the microenvironment, such as cytokines and growth factors, markedly inluencing disease progression and prognosis. his review will explore some of these components and mechanisms to demonstrate both underlying themes and the inherent complexity of these interacting systems in the initiation, progression, and metastasis of HBV-positive HCC. | Pengyuan Yang Geoffrey J.Markowitz Xiao-Fan Wang | 2014 | National Science Review2014，1，3： | 12 |
| 4 | Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer显示文摘Metastasis,the development of secondary malignant growths at a distance from a primary tumor,is the cause of death for 90%of cancer patients,but little is known about how metastatic cancer cells adapt to and colonize new tissue environments.Here,using clinical samples,patient-derived xenograft(PDX)samples,PDX cells,and primary/metastatic cell lines,we discovered that liver metastatic colorectal cancer(CRC)cells lose their colon-specific gene transcription program yet gain a liver-specific gene transcription program.We showed that this transcription reprogramming is driven by a reshaped epigenetic landscape of both typical enhancers and super-enhancers.Further,we identified that the liver-specific transcription factors FOXA2 and HNF1A can bind to the gained enhancers and activate the liver-specific gene transcription,thereby driving CRC liver metastasis.Importantly,similar transcription reprogramming can be observed in multiple cancer types.Our data suggest that reprogrammed tissue-specific transcription promotes metastasis and should be targeted therapeutically. | Shuaishuai Teng Yang Eric Li Ming Yang Rui Qi Yiming Huang Qianyu Wang Yanmei Zhang Shanwen Chen Shasha Li Kequan Lin Yang Cao Qunsheng Ji Qingyang Gu Yujing Cheng Zai Chang Wei Guo Pengyuan Wang Ivan Garcia-Bassets Zhi John Lu Dong Wang | 2020 | Cell Research2020，30，1： | 11 |
| 5 | Comparison of alternative extraction methods for secretome profiling in human hepatocellular carcinoma cells显示文摘Secreted proteins are important sources for early detection and diagnosis of disease, and as such have received considerable attention. The extraction of low concentration proteins from large volumes of culture media, which are rich in salts and other compounds that interfere with most proteomics techniques, presents a problem for secretome studies. Ultrafiltration, precipitation, and dialysis are three major extraction methods that can be used to overcome this problem. The present study for the first time, compared the merits and shortcomings of these three methods, without bias. Centrifugal ultrafiltration provided the best extraction efficiency, and precipitation provided the highest number of identifiable proteins. The three methods yielded closely related, but different, information on the secretome; thus, they should be considered complementary or, at least, supplementary methods. Three hundred and sixty unique proteins were identified, including 211 potential secreted proteins. Compared with previous studies, this study also identified 42 new secreted proteins. The present study not only offers a reference for the selection of secretome extraction methods, but also expands the secretome database for the investigation of hepatocellular carcinoma. | CAO Jing SHEN ChengPin ZHANG Jun YAO Jun SHEN HuaLi LIU YinKun LU HaoJie YANG PengYuan | 2011 | Science China(Life Sciences)2011，54，1： | 8 |
| 6 | Plasma membrane proteome analysis of the early effect of alcohol on liver: implications for alcoholic liver disease显示文摘在人,酒精的在消费上能导致严肃的肝疾病。在肝疾病上检验酒精的早效果,老鼠被给足够的乙醇开发肝肝硬化。在纤维变性的发作前的老鼠在这个工作被学习。肝的血浆膜(下午) 被提取由两次蔗糖密度坡度 centrifugation。从对待乙醇的老鼠和控制的下午的 proteome 侧面为相对、绝对的 quantitation (iTRAQ ) 用二维的胶化电气泳动(2-DE ) 和等压的标签被分析技术。乙醇处理改变了 15 不同的肝蛋白质的数量:他们中的 10 个被 2-DE 检测并且 5 由 iTRAQ。角质素 8 被两个方法检测。这些差别的基因本体论分析检测了显示的蛋白质他们的那个大多数涉及象有约束力的活动那样的重要房间函数(包括离子, DNA, ATP 绑定,等等) ,房间结构,或酶活动。在这些之中, annexin A2,角质素 8,和角质素 18 进一步用西方的污点分析被验证, annexin A2 被 immunohistochemistry 验证。我们的结果建议酒精有潜力由在 PM 改变几相关蛋白质的表示层次影响房间结构,粘附和酶活动。就我们的知识而言,这是第一次在肝下午 proteome 上学习酒精的效果,它可能对理解导致白酒的肝疾病的可能的机制有用。 | Lijun Zhang Xiaofang Jia Yanling Feng Xia Peng Zhiyong Zhang Wenjiang Zhou Zhanqing Zhang Fang Ma Xiaohui Liu Ye Zheng Pengyuan Yang Zhenghong Yuan | 2011 | Acta Biochimica et Biophysica Sinica2011，43，1： | 8 |
| 7 | Study on Missile Intelligent Fault Diagnosis System Based on Fuzzy NN Expert System显示文摘In order to study intelligent fault diagnosis methods based on fuzzy neural network (NN) expert system and build up intelligent fault diagnosis for a type of missile weapon system, the concrete implementation of a fuzzy NN fault diagnosis expert system is given in this paper. Based on thorough research of knowledge presentation, the intelligent fault diagnosis system is implemented with artificial intelligence for a large-scale missile weapon equipment. The method is an effective way to perform fuzzy fault diagnosis. Moreover, it provides a new way of the fault diagnosis for large-scale missile weapon equipment. | Yang Jun Feng Zhensheng Zhang Xien & Liu Pengyuan Dept. of Missile Engineering, Ordnance Engineering College, Shijiazhuang 050003, P. R. China | 2001 | Journal of Systems Engineering and Electronics2001，12，1： | 7 |
| 8 | Structural features and deformational ages of the northern Dabashan thrust belt显示文摘大规模弹出式结构发生在北 Dabashan 戳带(NDTB ) 的前面,由到南方的蘸 NNE Chengkou 差错的界限,和蘸 SSW Gaoqiao 指责到北方。弹出式结构作为构造活动的紧张的直接思考沿着它的罢工显示出不同特征。到西北,结构被形成积极象花的结构的一个二方向性的戳系统描绘。相反,东南的部分由垂直 Chengkou 差错和一系列指导 N 的 backthrusts 组成,显示出 semi-flower-like 结构。我们从 syntectonic microthermal 标明日期证明 Chengkou 指责的变形绢云母的 Ar-Ar 的现在的结果经历了强烈变丑在期间中间 -- 中生代 Yanshanian 历元(大约 143.3 妈) ,引起快速高举并且北 Dabashan 戳带推进。在这戳的繁殖期间,因为阻塞,一系列 backthrusts 从 Dabashan 戳带的边疆形成了,导致弹出式结构的发展。 | Pengyuan Li Jinjiang Zhang Lei Guo Xiongying Yang | 2012 | Geoscience Frontiers2012，3，1： | 4 |
| 9 | TGF-β-miR-34a-CCL22 Signaling-Induced Treg Cell Recruitment Promotes Venous Metastases of HBV-Positive Hepatocellular Carcinoma显示文摘 | Pengyuan Yang Qi-Jing Li Yuxiong Feng Yun Zhang Geoffrey J. Markowitz Shanglei Ning Yuezhen Deng Jiangsha Zhao Shan Jiang Yunfei Yuan Hong-Yang Wang Shu-Qun Cheng Dong Xie Xiao-Fan Wang | 2012 | Cancer Cell2012，，3： | 4 |
| 10 | Nuclear proteome profile of C57BL/6J mouse liver显示文摘The liver proteome can serve as a reference to better understand both disease mechanisms and possible therapeutics,since the liver is an important organ in the body that performs a large number of tasks.Here we identify the organelle proteome of C57BL/6J mouse liver nuclei as a promising strategy to enrich low abundance proteins,in the sense that analysis of whole liver cells is rather complex for current techniques and may not be suitable for proteins with low abundance.Evaluation of nucleus integrity and purity was performed to demonstrate the effectiveness of the optimized isolation procedure.The extracted nuclear proteins were identified by 2-DE MS analyses,and a total of 748 proteins were identified.Bioinformatic analyses were performed to demonstrate the physicochemical properties,cellular locations and functions of the proteins. | ZHANG Yang FANG CaiYun BAO HuiMin FAN HuiZhi SHEN HuaLi YANG PengYuan | 2013 | Science China(Life Sciences)2013，56，6： | 3 |
| 11 | EGF promotes mammalian cell growth by suppressing cellular senescence显示文摘 | Peter B Alexander Lifeng Yuan Pengyuan Yang Tao Sun Rui Chen Handan Xiang Jiekai Chen Haoyu Wu Daniel R Radiloff Xiao-Fan Wang | 2015 | Cell Research2015，25，1： | 3 |
| 12 | Advancements in mass spectrometry-based glycoproteomics and glycomics显示文摘Protein N-glycosylation plays a crucial role in a considerable number of important biological processes.Research studies on glycoproteomes and glycomes have already characterized many glycoproteins and glycans associated with cell development, life cycle, and disease progression. Mass spectrometry(MS) is the most powerful tool for identifying biomolecules including glycoproteins and glycans, however, utilizing MS-based approaches to identify glycoproteomes and glycomes is challenging due to the technical difficulties associated with glycosylation analysis. In this review, we summarize the most recent developments in MS-based glycoproteomics and glycomics, including a discussion on the development of analytical methodologies and strategies used to explore the glycoproteome and glycome, as well as noteworthy biological discoveries made in glycoproteome and glycome research. This review places special emphasis on China, where scientists have made sizeable contributions to the literature, as advancements in glycoproteomics and glycomincs are occurring quite rapidly. | Haojie Lu Ying Zhang Pengyuan Yang | 2016 | National Science Review2016，3，3： | 3 |
| 13 | Engineered exosomes from different sources for cancertargeted therapy显示文摘Exosome is a subgroup of extracellular vesicles,which has been serving as an efficient therapeutic tool for various diseases.Engineered exosomes are the sort of exosomes modified with surface decoration and internal therapeutic molecules.After appropriate modification,engineered exosomes are able to deliver antitumor drugs to tumor sites efficiently and precisely with fewer treatment-related adverse effects.However,there still exist many challenges for the clinical translation of engineered exosomes.For instance,what sources and modification strategies could endow exosomes with the most efficient antitumor activity is still poorly understood.Additionally,how to choose appropriately engineered exosomes in different antitumor therapies is another unresolved problem.In this review,we summarized the characteristics of engineered exosomes,especially the spatial and temporal properties.Additionally,we concluded the recent advances in engineered exosomes in the cancer fields,including the sources,isolation technologies,modification strategies,and labeling and imaging methods of engineered exosomes.Furthermore,the applications of engineered exosomes in different antitumor therapies were summarized,such as photodynamic therapy,gene therapy,and immunotherapy.Consequently,the above provides the cancer researchers in this community with the latest ideas on engineered exosome modification and new direction of new drug development,which is prospective to accelerate the clinical translation of engineered exosomes for cancer-targeted therapy. | Menghui Zhang Shengyun Hu Lin Liu Pengyuan Dang Yang Liu Zhenqiang Sun Bingbing Qiao Chengzeng Wang | 2023 | Signal Transduction and Targeted Therapy2023，8，4： | 2 |
| 14 | TiO_2-functionalized mesoporous materials for sensitive analysis of multi-phosphopeptides显示文摘Protein phosphorylation as one of the most important post-translational modifications in mammalian cells regulates numerous biological processes. Here we propose a novel strategy for the selective isolation and sensitive analysis of multi-phosphopeptides based on TiO2-gratfed mesoporous materials, in which MCM-41 and SBA-15 were chosen as the hard templates. The commercialized IMAC and TiO2 nanopartices were further investigated in the phosphopeptide analysis for comparison. The enrichment efficiency was evaluated and measured by MALDI-TOF mass spectrometry. The results indicated that both TiO2-SBA-15 and TiO2-MCM-41 exhibited the preferential affinity to multi-phosphopeptides compared with the other two widely used strategies. The mesoporous TiO2 based protocol showed highly selective and sensitive properties, where phosphopeptides could be identified at femtomole. | GUO WeiChao WAN JingJing QIAN Kun YU ChengZhong KONGJiLie YANG PengYuan LIU BaoHong | 2011 | Science China Chemistry2011，54，8： | 1 |
| 15 | Determination of three bioactive constituents in moutan cortex by capillary elec- trophoresis with electrochemical detection显示文摘 | Chen Gang Zhang Luyan Yang Pengyuan | 2005 | Analytical Sciences2005，21，： | 1 |
| 16 | Rapid Determination of Paeoniflorin and Three Sugars in Radix Paeoniae Alba by Capillary Electrophoresis显示文摘 | Gang CHEN Luyan ZHANG Pengyuan YANG | 2005 | Analytical Sciences2005，21，3： | 1 |
| 17 | New lysine-acetylated proteins screened by immunoaffinity and liquid chromatography-mass spectrometry显示文摘The lack of selective extraction specific for lysine-acetylated proteins has been a major problem in the field of acetylation biology,though acetylation plays a key role in many biological processes.In this paper,we report for the first time the proteomic screening of lysine-acetylated proteins from a mouse liver tissue,by a new approach of immunoaffinity purification of lysine-acetylated peptides combined with nano-HPLC/MS/MS analysis.We have found 20 lysine-acetylated proteins with 21 lysine-acetylated sites,among which 12 lysine-acetylated proteins and 16 lysine-acetylated sites have never been reported before.Notably,three acetyltransferases harboring in mitochondrion are newly discovered acetyltransferases responsible for the acetylation of nonhistone proteins.We have explored the significant patterns of residue preference by the hierarchical clustering analysis of amino acid residues surrounding acetylation sites,which could be helpful to the prediction of new sites of lysine acetylation.Our findings provide more candidates for studying the important roles played by acetylation in diverse cellular pathways and related human diseases. | ZHANG Bing ZHAO Chao LUO KaiXuan YAN GuoQuan YAO Jun WANG YingYin LU HaoJie FAN HuiZhi YANG PengYuan | 2010 | Science China Chemistry2010，53，1： | 1 |
| 18 | Magnetic loading of carbon nanotube/nano-Fe 3 O 4 composite for electrochemical sensing显示文摘 | Song Qu Joseph Wang Jilie Kong Pengyuan Yang Gang Chen | 2006 | Talanta2006，，3： | 1 |
| 19 | Rapid determination of paeoniflorin and three sugars in radix paeoniae alba by capillary electrophoresis显示文摘 | Gang Chen Luyan Zhang Pengyuan Yang | 2005 | Analytical Sciences2005，21，： | 1 |
| 20 | Tumor-derived secretory clusterin induces epithelial–mesenchymal transition and facilitates hepatocellular carcinoma metastasis显示文摘 | Cun Wang Kai Jiang Xiaonan Kang Dongmei Gao Chun Sun Yan Li Lu Sun Shu Zhang Xiaohui Liu Weizhong Wu Pengyuan Yang Kun Guo Yinkun Liu | 2012 | International Journal of Biochemistry and Cell Biology2012，，12： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员