|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Function of apoptosis and expression of the proteins Bcl-2,p53 and C-myc in the development of gastric cancer显示文摘INTRODUCTIONIn China ,the incidence and mortality of gastric cancer rank the second among all cancers. Recent development of cancer [1-20].The aim of this study was investigat the insight of apoptosis and bcl-2, p53 and C-myc protein expression in the development of gastric cancer . | An Gao Xu Shao Guang Li Ji Hong Liu Ai Hua Gan Research Laboratory of Digestive Disease,Huizhou Central People’s Hospital,Huizhou 516001,Guangdong Province,ChinaDr.An Gao Xu graduated from Guangdong Medical College in 1984.He is an associate physician-in-chief,specializing in the research and treatment of gastrointestinal and liver tumors.He has published 24 papers and 1 book. | 2001 | World Journal of Gastroenterology2001，7，3： | 91 |
| 2 | Th e role of ferroptosis in ionizing radiation-induced cell death and tumor suppression显示文摘Ferroptosis,a form of regulated cell death caused by lipid peroxidation,was recently identified as a natural tumor suppression mechanism.Here,we show that ionizing radiation(IR)induces ferroptosis in cancer cells.Mechanistically,IR induces not only reactive oxygen species(ROS)but also the expression of ACSL4,a lipid metabolism enzyme required for ferroptosis,resulting in elevated lipid peroxidation and ferroptosis.ACSL4 ablation largely abolishes IR-induced ferroptosis and promotes radioresistance.IR also induces the expression of ferroptosis inhibitors,including SLC7A11 and GPX4;as an adaptive response.IR-or KEAP1 deficiencyinduced SLC7A11 expression promotes radioresistance through inhibiting ferroptosis.Inactivating SLC7A11 or GPX4 with ferroptosis inducers(FINs)sensitizes radioresistant cancer cells and xenograft tumors to IR.Furthermore,radiotherapy induces ferroptosis in cancer patients,and increased ferroptosis correlates with better response and longer survival to radiotherapy in cancer patients.Our study reveals a previously unrecognized link between IR and ferroptosis and indicates that further exploration of the combination of radiotherapy and FINs in cancer treatment is warranted. | Guang Lei Yilei Zhang Pranavi Koppula Xiaoguang Liu Jie Zhang Steven HLin Jaffer AAjani Qin Xiao Zhongxing Liao Hui Wang Boyi Gan | 2020 | Cell Research2020，30，2： | 88 |
| 3 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 4 | Comparing the Diagnostic Criteria for Gestational Diabetes Mellitus of World Health Organization 2013 with 1999 in Chinese Population显示文摘INTRODUCTION Gestational diabetes mellitus (GDM) was earlier defined as 'hyperglycemia first recognized during pregnancy' and has more recently been described by American Diabetes Association (ADA) (2012) as diabetes diagnosed during pregnancy that is not clearly overt diabetes.[1] The hyperglycemia and adverse pregnancy outcomes study (HAPO) demonstrated that the risk of adverse maternal,fetal,and neonatal outcomes continuously increase as a function of maternal glycemia at 24-28 weeks,even within ranges previously considered normal for pregnancy.[2] After reviewing the results of the HAPO study,many international diabetes study groups,including the International Association of Diabetes and Pregnancy Study Groups and ADA have adopted the 75 g oral glucose tolerance test (OGTT) at 24-28 weeks as a screening and diagnostic test and defined new cut-off values for GDM diagnosis.The Ministry of Health of China published the criteria for GDM,that is the same as the ADA criteria on July 1,2011.World Health Organization (WHO) has recommended the 75 g OGTT with different cut-offvalues as a diagnostic test since 1999,changed its recommendation on 2013.In Hongkong and Yunnan province in China,most hospital adopted the WHO 1999 criteria,nevertheless,there is no evidence of multicenter and large sample to show that the GDM population within the same ethnic group diagnosed by the new WHO criteria matches those diagnosed by the old one and consistency between the two criteria has not been reported.Thus,we conducted this study to analyze the two criteria in Chinese pregnant women. | Weiwei Zhu Huixia Yang Yumei Wei Zilian Wang Xuelan Li Hairong Wu Nan Li Meihua Zhang Xinghui Liu Hua Zhang Yunhui Wang Jianmin Niu Yujie Gan Liruo Zhong Yunfeng Wang Anil Kapur | 2015 | Chinese Medical Journal2015，，1： | 42 |
| 5 | Plasma exchange-centered artificial liver support system in hepatitis B virus-related acute-onchronic liver failure:a nationwide prospective multicenter study in China显示文摘BACKGROUND:Plasma exchange(PE)-centered artificial liver support system reduced the high mortality rate of hepatitis B virus(HBV)-related acute-on-chronic liver failure(ACLF).But the data were diverse in different medical centers.The present prospective nationwide study was to evaluate the effects of PE on patients with HBV-ACLF at different stages.METHODS:From December 2009 to December 2011,we evaluated 250 patients at different stages of HBV-ACLF from 10 major medical centers in China.All the laboratory parameters were collected at admission,before and after PE.RESULTS:Among the 250 patients who underwent 661 rounds of PE,one-month survival rate was 61.6%; 141(56.4%) showed improvement after PE.Variables such as age(P=0.000),levels of total bilirubin(TB,P=0.000),direct bilirubin(P=0.000),total triglycerides(P=0.000),low-density lipoprotein(P=0.022),Na+(P=0.014),Cl–(P=0.038),creatinine(Cr,P=0.007),fibrinogen(P=0.000),prothrombin time(PT,P=0.000),white blood cell(P=0.000),platelet(P=0.003) and MELD(P=0.000) were significantly related to prognosis.Multivariate logistic regression analysis showed that age,disease stage,TB,Cr and PT levels were independent risk factors of mortality among HBV-ACLF patients.CONCLUSIONS:PE can improve the clinical outcome of patients with HBV-ACLF.Levels of TB,Cr and PT,age and disease stage help to predict prognosis. | Jia-Jia Chen Jian-Rong Huang Qian Yang Xiao-Wei Xu Xiao-Li Liu Shao-Rui Hao Hui-Fen Wang Tao Han Jing Zhang Jian-He Gan Zhi-Liang Gao Yu-Ming Wang Shu-Mei Lin Qing Xie Chen Pan Lan-Juan Li | 2016 | Hepatobiliary & Pancreatic Diseases International2016，15，3： | 48 |
| 6 | Colorectal cancer in Guangdong Province of China:A demographic and anatomic survey显示文摘AIM:To determine the basic demographic features of colorectal cancer (CRC) in five hospitals located in four different areas of Guangdong Province,China.METHODS:A review of patient records from 1986 to 2006 from five hospitals was conducted. Patient data was obtained,including age,gender,location of le-sions,staging and histological type of CRC. The Chi-square test was used to assess differences in rates and a significance level of 0.05 was used. Univariate com-parisons were made via Fisher's exact tests.RESULTS:Analysis was carried out on 8172 CRC patents,6.1% (499/8172) of the patients were aged ≤ 30 years. The peak incidence was between the ages 61-70 years (27.8%). The mean age at CRC diagnosis increased from 52 years (1986-1988) to 60 years(2004-2006) and the proportion of young CRC patients decreased from 8.0% to 5.9% over the same period. Of 8172 lesions,4434 (54.3%) were located in rectum and 3738 (45.7%) in colon. The incidence of rectal cancer decreased significantly from 59.4% (1989-1991) to 51.8% (2004-2006) and right sided colon cancer increased from 40.6% to 48.2%. The mean age,ana-tomic distribution,histological type and differentiation degree were significantly different among the four geographical areas (P < 0.05).CONCLUSION:The hospitalization rate for CRC has increased in Guangdong in recent years. The characteristics of CRC from the five hospitals located in the four different areas of Guangdong Province are also different. Further studies are needed to assess more recent trend in the incidence and prevalence of CRC as well as the respective roles of genetic and environ-mental factors in CRC. | Xu, An-Gao Yu, Zhi-Jin Jiang, Bo Wang, Xin-Ying Zhong, Xu-Hui Liu, Ji-Hong Lou, Qiu-Yun Gan, Ai-Hua | 2010 | World Journal of Gastroenterology2010，16，8： | 40 |
| 7 | Effects of mixture ratio on anaerobic co-digestion with fruit and vegetable waste and food waste of China显示文摘The biochemical methane potentials for typical fruit and vegetable waste (FVW) and food waste (FW) from a northern China city were investigated,which were 0.30,0.56 m 3 CH 4/kgVS (volatile solids) with biodegradabilities of 59.3% and 83.6%,respectively.Individual anaerobic digestion testes of FVW and FW were conducted at the organic loading rate (OLR) of 3 kg VS/(m 3 ·day) using a lab-scale continuous stirred-tank reactor at 35℃.FVW could be digested stably with the biogas production rate of 2.17 m 3/(m 3 ·day) and methane production yield of 0.42 m 3 CH 4/kg VS.However,anaerobic digestion process for FW was failed due to acids accumulation.The effects of FVW:FW ratio on co-digestion stability and performance were further investigated at the same OLR.At FVW and FW mixing ratios of 2:1 and 1:1,the performance and operation of the digester were maintained stable,with no accumulation of volatile fatty acids (VFA) and ammonia.Changing the feed to a higher FW content in a ratio of FVW to FW 1:2,resulted in an increase in VFAs concentration to 1100-1200mg/L,and the methanogenesis was slightly inhibited.At the optimum mixture ratio 1:1 for co-digestion of FVW with FW,the methane production yield was 0.49 m 3 CH 4/kg VS,and the volatile solids and soluble chemical oxygen demand (sCOD) removal efficiencies were 74.9% and 96.1%,respectively. | Jia Lin Jiane Zuo Lili Gan Peng Li Fenglin Liu Kaijun Wang Lei Chen Hainan Gan | 2011 | Journal of Environmental Sciences2011，23，8： | 36 |
| 8 | Morphological study on colonic pathology in ulcerative colitis treated by moxibustion显示文摘AIM To observe the therapeutic effect ofmoxibustion on ulcerative colitis and itsinfluence on the colonic mucosal morphology.METHODS Forty-six patients with ulcerativecolitis were randomly divided into themoxibustion with herbal medicine underneathgroup and the western medicine group.Thirtypatients were treated with the abovemoxibustion and 16 patients with Salicylayefapyridine(SASP).The colonic mucosa of 13patients in the moxibustion group was observedby colonoscopy before and after the treatment.Mucin was also analyzed by H.E and AB-PASstaining.RESULTS Seventeen patients were clinicallycured,12 were improved and 1 unchanged in themoxibustion group.In the control group,5patients were clinically cured,7 improved and 4unchanged.Thirteen patients with active UCwere taken as the subjects for histopathologicanalysis in this study.The colonic mucosallesions were remarkably improved and thecharacteristic of the mucin also changed.Inmost sections,the chronic inflammation ofmucosa was geatly ameliorated(P<0.01).Theinflammatory cell infiltratation much decreased and neutrophils,disapeared in most sections(P<0.001).The goblet cells significantlyincreased(P<0.001);crypt paracrypt abscessor mucosal ulceration was seen(P<0.001).CONCLUSION The rate of cure of ulcerative colitis by moxibustion with herbal medicine beneath is superior to that by SASP. This sort of moxibustion can effectively improve the colonic mucosal lesions and restore the proportion of mucoprotein to near normal. | Huan Gan Wu Li Bin Zhou Da Ren Shi Shi Min Liu Hui Rong Liu Bi Meng Zhang Han Ping Chen Lin Shan Zhang | 2000 | World Journal of Gastroenterology2000，6，6： | 28 |
| 9 | The effect of oxidative stress in myocardial cell injury in mice exposed to chronic intermittent hypoxia显示文摘背景妨碍的睡觉呼吸暂停症候群(OSAS ) 是为心血管的疾病的一个重要风险因素。长期的断断续续的组织缺氧(CIH ) 被认为是在 OSA 病人的心血管的疾病的最重要的原因之一。这个重复组织缺氧和氧化周期类似于 hypoxia-reperfusion 损害,它开始氧化应力。在这研究,我们观察了 CIH 和 N-acetylcysteine (NAC ) 的效果导致的 cardiocytes 损害。三十只 ICR 老鼠随机被分到 3 个组的方法:控制, CIH 和 NAC (CIH+NAC ) 组。Malondialdehyde (MDA ) 和 cardiocyte homogenates 的 superoxide dismutase (草皮) 被测量。浆液类脂化合物被一个仪器方法测量。浆液心脏的 troponin (cTnl ) 我被连接酶的 immunosorbent 试金(ELISA ) 检测。心肌层病理学的节是草皮活动和在 CIH 的 cardiocyte homogenates 的 MDA 集中组织的 observed.Results (1 ) 比在另外的组显著地高(P < 0.005 ) 。NAC 组的 MDA 集中比控制组的低(P < 0.01 ) 。(2 ) CIH 和 NAC 组的浆液 cTnl 集中比控制组的显著地高(P < 0.01 ) 。(3 ) 在 NAC 组的浆液 triglyceride 层次比在另外的组低(P < 0.01 ) ,当在低密度脂蛋白和高密度没有重要差别时,在三之中的脂蛋白组织。(4 ) 心肌层,模糊的横向的有条纹或刻痕之状态,和织物流出的退化在 CIH 和 NAC 组在织物节被观察。然而,正常织物在 CIH 导致的氧化压力能伤害的控制 group.Conclusion 被发现 cardiocytes 和损害效果罐头被 NAC 部分禁止。 | LIU Jian-nan ZHANG Jie-xin LU gan QIU Yan YANG Di YIN Guo-yong ZHANG Xi-long | 2010 | Chinese Medical Journal2010，，1： | 25 |
| 10 | Immune Responses to the Attenuated Mycoplasma hyopneumoniae 168 Strain Vaccine by Intrapulmonic Immunization in Piglets显示文摘To investigate the immune responses to the attenuated Mycoplasma hyopneumoniae 168 strain vaccine, 8-15 d old piglets were immunized with M. hyopneumoniae 168 strain vaccine by intrapulmonic route. And the specific IgG antibody in serum, lymphoproliferation, IFNγ, and specific secretory IgA (SIgA) antibody in bronchoalveolar lavage fluid were detected on 30 and 60 d post-immunization (DPI), respectively. On 60 DPI, all the pigs except for those in health control group were challenged with a field M. hyopneumoniae strain JS. Necropsy was performed on 30 d post-challenge (DPC). The results showed that IFNγ and specific SIgA were stimulated on surface of respiratory tract after immunization. And peripheral blood mononuclear cells could also be proliferated about 1.81 and 2.12 fold on 30 and 60 DPI when stimulated by M. hyopneumoniae protein in vitro. However, no serum IgG antibody against M. hyopneumoniae was detected during the whole immune phage. After challenge, vaccinated pigs were observed with only very slight histological lesion in individual lobes. None of vaccinated pigs showed any clinical signs. While the unvaccinated pigs from challenge control group showed varying degrees of clinical sign and severe macroscopical lesion of mycoplasmal pneumonia of swine (MPS). The result suggested that the attenuated M. hyopneumoniae 168 strain vaccine inoculated by intrapulmonic route could activate the systemic cellular immunity, the local mucosal immunity and IFNγ secretion in respiratory tract to against M. hyopneumoniae infection in piglets. | FENG Zhi-xin, SHAO Guo-qing, LIU Mao-jun, WU Xu-su, ZHOU Yong-qi and GAN Yuan Key Laboratory of Animal Diseases Diagnostic and Immunology, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences/National Center for Engineering Research of Veterinary Bio-Products, Ministry of Agriculture, Nanjing 210014, P.R.China | 2010 | Agricultural Sciences in China2010，9，3： | 22 |
| 11 | Genome analysis of Taraxacum kok-saghyz Rodin provides new insights into rubber biosynthesis显示文摘The Russian dandelion Taraxacum kok-saghyz Rodin(TKS), a member of the Composite family and a potential alternative source of natural rubber(NR) and inulin, is an ideal model system for studying rubber biosynthesis. Here we present the draft genome of TKS, the first assembled NR-producing weed plant. The draft TKS genome assembly has a length of 1.29 Gb, containing 46 731 predicted protein-coding genes and68.56% repeats, in which the LTR-RT elements predominantly contribute to the genome enlargement. We analyzed the heterozygous regions/genes, suggesting its possible involvement in inbreeding depression.Through comparative studies between rubber-producing and non-rubber-producing plants, we found that enzymes of the mevalonate(MVA) pathway and rubber elongation might be critical for rubber biosynthesis, and several key isoforms have been isolated and shown to be predominantly expressed in the latex, indicating their crucial functions in rubber biosynthesis. Moreover, for two important families in rubber elongation, the CPT/CPTL and REF/SRPP families, diverse evolutionary tracks have been revealed. These results provide valuable resources and new insights into the mechanism of NR biosynthesis,and facilitate the development of alternative NR-producing crops. | Tao Lin Xia Xu Jue Ruan Shizhong Liu Shigang Wu Xiujuan Shao Xiaobo Wang Lin Gan Bi Qin Yushuang Yang Zhukuan Cheng Suhua Yang Zhonghua Zhang Guosheng Xiong Sanwen Huang Hong Yu Jiayang Li | 2018 | National Science Review2018，5，1： | 22 |
| 12 | Paleogene Tectonic Evolution Controls on Sequence Stratigraphic Patterns in the Central Part of Deepwater Area of Qiongdongnan Basin, Northern South China Sea显示文摘In active rift basins, tectonism is extremely important for sequence stratigraphic patterns, affecting both the sequence architecture and internal makeup. Sequence stratigraphic framework of a Paleogene rift succession in Qiongdongnan Basin, northern South China Sea, was built using seismic profiles, complemented by well logs and cores. One first-order and three second-order sequences were identified on the basis of basin-scale unconformities, and seven third-order sequences are defined by unconformities along the basin margins and correlative conformities within the central basin. Through unconformity analysis and backstripping procedure, the Paleogene synrift tectonic evolution of deepwater area of Qiongdongnan Basin was proved to be episodic, which can be divided into rifting stage-I, rifting stage-II and rifting stage-III. Episodic rifting resulted in the formation of various types of structural slope break belts, which controlled different architectures and internal makeup of sequences. This study enhances the understanding of the control of tectonic evolution on sequence stratigraphic patterns and establishes relevant patterns in a typical rift basin, and further proposes the favorable sandstone reservoirs developing in different sequence stratigraphic patterns, which will be pretty helpful for subtle pool exploration in deepwater area of petroliferous basins. | Guangzeng Song Hua Wang Huajun Gan Zhipeng Sun Xiaolong Liu Meng Xu Jinfeng Ren Ming Sun Di Sun | 2014 | Journal of Earth Science2014，25，2： | 16 |
| 13 | Characteristics of strong earthquake evolution around the eastern boundary faults of the Sichuan-Yunnan rhombic block显示文摘Based on the existing materials of fault segmentation,characteristic earthquakes,and their empirical relationships,we calculated the parameters of the fault segments,such as length,width,magnitudes of characteristic earthquakes,etc.Constrained by GPS velocity field,the slip rates of these fault segments in depth were inversed using the 3-D half-space elastic dislocation model.As not all of the recurrence periods and co-seismic displacements of characteristic earthquakes are known,we selected the fault segments with these two parameters known and calculated the accumulation rate of average co-seismic displacement,which shows the faults' slip rate in seismogenic layer.Then,the slip rate in depth was compared with that in seismogenic layer,the relationship between them was obtained,and this relationship was used to get the recurrence periods and co-seismic displacements of all fault segments.After the studies above,we calculated the co-seismic deformation field of all the earthquakes larger than M s 6.8 from AD 1700 one by one and inversed the potential displacement in the co-seismic deformation field.Then,we divided the potential displacement by the slip rate from GPS inversion to get the influences of these fault segments,added the influences into the elapsed time of the characteristic earthquakes,and obtained the earthquake hazard degree of all the segments we studied in the form of the ratio of elapsed time to recurrence period;so,we name the ratio as the Impending Earthquake Risk (IER).Historical earthquake cases show that the fault segment is in safety when the IER is less than 1 but in danger after the IER becomes larger than 1.In 2009,the IER is larger than 1 on the following segments,1.35 on the Tagong segment of Xianshuihe fault,1 on the Menggu-Dongchuan segment,1.04 on the Dongchuan-Xundian segment,and 1.09 on the Yiliang-Chengjiang segment of Xiaojiang fault. | CHENG Jia LIU Jie GAN WeiJun YU HuaiZhong LI Gang | 2011 | Science China Earth Sciences2011，54，11： | 15 |
| 14 | Elevated nocturnal and morning blood pressure in patients with obstructive sleep apnea syndrome显示文摘 | HE Quan-ying FENG Jing ZHANG Xilong LIANG Zong-an HUANG Shao-guang KANG Jian WANG Guang-fa ZHANG Li-qiang MA Li-jun WANG Bei LIN Qi-chang ZHANG Jin-nong LIU Hui-guo LUO Yuan-ming LIU Jian-hong WANG Shi XIAO Gao-hui LU Gan ZHANG Jin FENG Xue-wei CHEN Bao-yuan | 2012 | Chinese Medical Journal2012，，10： | 15 |
| 15 | Ionic composition of submicron particles(PM_(1.0)) during the long-lasting haze period in January 2013 in Wuhan, central China显示文摘In January 2013, a long-lasting severe haze episode occurred in Northern and Central China; at its maximum, it covered a land area of approximately 1.4 million km2. In Wuhan, the largest city in Central China, this event was the most severe haze episode in the 21st century. Aerosol samples of submicron particles(PM1.0) were collected during the long-lasting haze episode at an urban site and a suburban site in Wuhan to investigate the ion characteristics of PM1.0 in this area. The mass concentrations of PM1.0 and its water-soluble inorganic ions(WSIIs) were almost at the same levels at two sites, which indicates that PM1.0 pollution occurs on a regional scale in Wuhan. WSIIs(Na+, NH+ 4, K+, Mg2+, Ca2+, Cl-, NO-3and SO2-4) were the dominant chemical species and constituted up to 48.4% and 47.4% of PM1.0 at WD and TH, respectively. The concentrations of PM1.0 and WSIIs on haze days were approximately two times higher than on normal days. The ion balance calculations indicate that the particles were more acidic on haze days than on normal days. The results of the back trajectory analysis imply that the high concentrations of PM1.0 and its water-soluble inorganic ions may be caused by stagnant weather conditions in Wuhan. | Hairong Cheng Wei Gong Zuwu Wang Fan Zhang Xinming Wang Xiaopu Lv Jia Liu Xiaoxin Fu Gan Zhang | 2014 | Journal of Environmental Sciences2014，26，4： | 15 |
| 16 | Machine learning in materials genome initiative:A review显示文摘Discovering new materials with excellent performance is a hot issue in the materials genome initiative.Traditional experiments and calculations often waste large amounts of time and money and are also limited by various conditions. Therefore, it is imperative to develop a new method to accelerate the discovery and design of new materials. In recent years, material discovery and design methods using machine learning have attracted much attention from material experts and have made some progress. This review first outlines available materials database and material data analytics tools and then elaborates on the machine learning algorithms used in materials science. Next, the field of application of machine learning in materials science is summarized, focusing on the aspects of structure determination, performance prediction, fingerprint prediction, and new material discovery. Finally, the review points out the problems of data and machine learning in materials science and points to future research. Using machine learning algorithms, the authors hope to achieve amazing results in material discovery and design. | Yingli Liu Chen Niu Zhuo Wang Yong Gan Yan Zhu Shuhong Sun Tao Shen | 2020 | Journal of Materials Science & Technology2020，54，22： | 15 |
| 17 | miR-374b-5p suppresses RECK expression and promotes gastric cancer cell invasion and metastasis显示文摘AIM:To profile expression of micro RNAs(mi RNAs)in gastric cancer cells and investigate the effect of mi R-374b-5p on gastric cancer cell invasion and metastasis.METHODS:An mi RNA microarray assay was performed to identify mi RNAs differentially expressed in gastric cancer cell lines(MGC-803 and SGC-7901)compared with a normal gastric epithelial cell line.Upregulation of mi R-374b-5p was newly identified and confirmed via quantitative real-time reverse transcriptionPCR(q RT-PCR).MGC-803 cells were transfected with a synthesized anti-mi R-374b-5p sequence or a control vector using Lipofectamine reagent,or treated with transfection reagent alone or phosphate-buffered saline as controls.Rate of transfection was verified after 48 h by q RT-PCR.Cells were then subjected to transwell migration,wound scratch and cell counting kit-8 assays.A bioinformatic analysis to identify mi R-374b-5p target genes was performed using mi Randa,Pic Tar and Target Scan software.A dual luciferase reporter assay was performed to evaluate the influence of mi R-374b-5p on target gene activation,and q RT-PCR and Western blot were used to evaluate the levels of target m RNA and protein following transfection with mi R-374b-5p antisense oligonucleotides.RESULTS:The microarray profiling revealed downregulation of 14(fold change<0.667;P<0.05)and upregulation of 12(fold change>1.50;P<0.05)mi RNAs in MGC-803 and SGC-7901 cells compared with GES-1 controls.The upregulation of mi R-374b-5p(fold change=1.75 and 1.64 in MGC-803 and SGC-7901,respectively;P<0.05)was confirmed by q RT-PCR.Compared with the control groups,the restoration of mi R-374b-5p expression with anti-mi R-374b-5p significantly suppressed the metastasis,invasion and proliferation of MGC-803 cells.The bioinformatic analysis predicted that the 3’untranslated region(UTR)of reversion-inducing cysteine-rich protein with Kazal motif(RECK)contains three mi R-374b-5p target sequences.RECK was verified as a target gene in a dual luciferase reporter assay showing that activation of RECK 3’UTR-pmir GLO was increased by co-transfection with mi R-374b-5p.Finally,transfection of mi R-374b-5p antisense oligonucleotides increased m RNA and protein levels of RECK in MGC-803cells(P<0.05).CONCLUSION:These findings indicate that upregulation of mi R-374b-5p contributes to gastric cancer cell metastasis and invasion through inhibition of RECK expression. | Juan Xie Zhi-Hui Tan Xia Tang Ming-Shu Mo Yan-Ping Liu Run-Liang Gan Yi Li Li Zhang Guo-Qing Li | 2014 | World Journal of Gastroenterology2014，20，46： | 15 |
| 18 | In vitro antibacterial activity of thymol and carvacrol and their effects on broiler chickens challenged with Clostridium perfringens显示文摘Background:In the post-antibiotic era,essential oils(EO) are promising alternatives to growth-promoting antibiotics.The aim of the present study was to investigate the antibacterial activities of an EO product and its components thymol and carvacrol in vitro,and the efficacy of EO to control Clostridium perfringens challenge in broiler chickens.Results:The in vitro minimum inhibitory concentration assay showed strong antibacterial activity of the EO product,thymol,and carvacrol against pathogenic Escherichia coli,C.perfringens,and Salmonella strains,and weak activity towards beneficial Lactobacillus strains.Besides,an additive effect was observed between thymol and carvacrol.The in vivo study was carried out with 448 male broiler chicks following a 4 × 2 factorial arrangement to test the effects of EO supplementation(0,60,120,or 240 mg/kg EO in wheat-based diet),pathogen challenge(with or without oral gavage of C perfringens from day 14 to day 20) and their interactions.Each treatment consisted of eight replicate pens(seven birds/pen).The challenge led to macroscopic gut lesions,and resulted in a significant increase in ileal populations of C.perfringens and Escherichia subgroup(P< 0.05) on day 21.Dietary EO supplementation did not influence C perfringens numbers,but linearly alleviated intestinal lesions on day 21 and 28(P=0.010 and 0.036,respectively),and decreased Escherichia populations in ileum with increased EO dosages(P=0.027 and 0.071 for day 21 and 28,respectively).For caecum,EO quadratically influenced Lactobacillus populations on day 21(p=0.002),and linearly decreased the numbers of total bacteria and Escherichia on day 28(P=0.026 and 0.060,respectively).Mean thymol and carvacrol concentrations in the small intestine were 0.21 and 0.20 μg/g in intestinal digesta(wet weight),respectively,for birds fed 60 mg/kg EO,and 0.80 and 0.71 μg/g,respectively,for birds fed 240 mg/kg EO.Conclusions:These results indicated that dietary EO supplementation could affect intestinal microbiota and alleviate intestinal lesions in broilers,which may contribute in controlling C.perfringens infection in broiler chickens. | Encun Du Liping Gan Zhui Li Weiwei Wang Dan Liu Yuming Guo | 2016 | Journal of Animal Science and Biotechnology2016，7，1： | 14 |
| 19 | A Review of Modeling Approaches and Tools for the Off-design Simulation of Organic Rankine Cycle显示文摘Organic Rankine Cycles(ORCs) are an effective way to produce electricity from low-grade heat sources, which cannot be effectively obtained using conventional high-temperature Rankine cycles. Due to the lack of available information regarding the real Organic Rankine Cycle units on industrial level, off-design simulation under diversified operating conditions plays a significant role for both the system performance prediction and control strategy design. This paper summarizes the theoretical basis, modeling approaches and tools for ORC off-design simulations. Firstly, a review was conducted on the individual state-of-the-art convective heat transfer correlations and void fraction models. Secondly, different kinds of modeling approaches and simulation tools were proposed, highlighting their relevant characteristics, and were categorized for their specific applications. Moreover, an in-depth analysis of technical challenges related to various applications and focusing on the model accuracy and complexity, computational efficiency, as well as the model compatibility were extensively described and discussed. Finally, the current research trends in this field and the development for further investigations were presented. | LIU Liuchen ZHU Tong GAO Naiping GAN Zhongxue | 2018 | Journal of Thermal Science2018，27，4： | 14 |
| 20 | Genotypic Characterization of Methicillin-resistant Staphylococcus aureus Isolated from Pigs and Retail Foods in China显示文摘Objective To investigate the genotypic diversity of Methicillin-resistant Staphylococcus aureus(MRSA) isolated from pigs and retail foods from different geographical areas in China and further to study the routes and rates of transmission of this pathogen from animals to food. Methods Seventy-one MRSA isolates were obtained from pigs and retail foods and then characterized by multi-locus sequencing typing(MLST), spa typing, multiple-locus variable number of tandem repeat analysis(MLVA), pulsed-field gel electrophoresis(PFGE), and antimicrobial susceptibility testing. Results All isolated MRSA exhibited multi-drug resistance(MDR). Greater diversity was found in food-associated MRSA(7 STs, 8 spa types, and 10 MLVA patterns) compared to pig-associated MRSA(3 STs, 1 spa type, and 6 MLVA patterns). PFGE patterns were more diverse for pig-associated MRSA than those of food-associated isolates(40 vs. 11 pulse types). Among the pig-associated isolates, CC9-ST9-t899-MC2236 was the most prevalent clone(96.4%), and CC9-ST9-t437-MC621(20.0%) was the predominant clone among the food-associated isolates. The CC9-ST9 isolates showed significantly higher antimicrobial resistance than other clones. Interestingly, CC398-ST398-t034 clone was identified from both pig-and food-associated isolates. Of note, some community-and hospital-associated MRSA strains(t030, t172, t1244, and t4549) were also identified as food-associated isolates. Conclusion CC9-ST9-t899-MC2236-MDR was the most predominant clone in pigs, but significant genetic diversity was observed in food-associated MRSA. Our results demonstrate the great need for improved surveillance of MRSA in livestock and food and effective prevention strategies to limit MDR-MRSA infections in China. | WANG Wei LIU Feng ZULQARNAIN Baloch ZHANG Cun Shan MA Ke PENG Zi Xin YAN Shao Fei HU Yu Jie GAN Xin DONG Yin Ping BAI Yao LI Feng Qin YAN Xiao Mei MA Ai Guo XU Jin | 2017 | Biomedical and Environmental Sciences2017，30，8： | 13 |
您所在的机构暂未开通此部分的功能，请联系管理员