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| 1 | Effects of chemical modification on physicochemical properties and adsorption behavior of sludge-based activated carbon显示文摘This study aimed to explore the adsorption performance of sludge-based activated carbon(SBC)towards dissolved organic matters(DOMs)removal from sewage,and investigated the modification effect of different types of chemicals on the structure of synthesized SBC.Waste activated sludge(WAS)was used as a carbon source,and HCl,HNO 3,and Na OH were used as different types of chemicals to modify the SBC.With the aid of chemical activation,the modified SBC showed higher adsorption performances on DOMs removal with maximum adsorption of 29.05 mg/g and second-order constant(k)of 0.1367(L/mol/sec)due to the surface elution of ash and minerals by chemicals.The surface elemental composition of MSBC suggested that the content of C-C and C-O functional groups on the surface of modified sludge-based activated carbon(MSBC)played an important role on the adsorption capacities of MSBC towards DOMs removal in sewage.Additionally,the residual molecular weight of DOMs in sewage was investigated using a 3-dimension fluorescence excitationemission matrix(3 D-EEM)and high-performance size exclusion chromatography(HP-SEC).Results showed that the chemical modification significantly improved the adsorption capacity of MSBC on humic acids(HA)and aromatic proteins(APN),and both of Na OH-MSBC and HCl-MSBC were effective for a wide range of different AMW DOMs removal from sewage,while the HNO 3-MSBC exhibited poorly on AMW organics of 2,617 Da and 409 Da due to the reducing content of macropore.In brief,this study provides reference values for the impact of the chemicals of the activation stage before the SBCs application. | Chunxu Wu Lanfeng Li Hao Zhou Jing Ai Hongtao Zhang Jialin Tao Dongsheng Wang Weijun Zhang | 2021 | Journal of Environmental Sciences2021，33，2： | 7 |
| 2 | Dynamic culture of a thermosensitive collagen hydrogel as an extracellular matrix improves the construction of tissue-engineered peripheral nerve显示文摘Tissue engineering technologies offer new treatment strategies for the repair of peripheral nerve injury, but cell loss between seeding and adhesion to the scaffold remains inevitable. A thermosensitive collagen hydrogel was used as an extracellular matrix in this study and combined with bone marrow mesenchymal stem cells to construct tissue-engineered peripheral nerve composites in vitro. Dynamic culture was performed at an oscillating frequency of 0.5 Hz and 35° swing angle above and below the horizontal plane. The results demonstrated that bone marrow mesenchymal stem cells formed membrane-like structures around the poly-L-lactic acid scaffolds and exhibited regular alignment on the composite surface. Collagen was used to fill in the pores, and seeded cells adhered onto the poly-L-lactic acid fibers. The DNA content of the bone marrow mesenchymal stem cells was higher in the composites constructed with a thermosensitive collagen hydrogel compared with that in collagen I scaffold controls. The cellular DNA content was also higher in the thermosensitive collagen hydrogel composites constructed with the thermosensitive collagen hydrogel in dynamic culture than that in static culture. These results indicate that tissue-engineered composites formed with thermosensitive collagen hydrogel in dynamic culture can maintain larger numbers of seeded cells by avoiding cell loss during the initial adhesion stage. Moreover, seeded cells were distributed throughout the material. | Lanfeng Huang Rui Li Wanguo Liu Jin Dai Zhenwu Du Xiaonan Wang Jianchao Ma Jinsong Zhao | 2014 | Neural Regeneration Research2014，9，14： | 3 |
| 3 | Both structure and function of human monoclonal antibodies contribute to enhancement of Zika virus infectivity in vitro显示文摘Dear Editor,Antibody-dependent enhancement(ADE)has long been recognized for dengue virus(DENV)in vitro and in vivo.It is now clear that antibodies to DENV can also enhance Zika virus(ZIKV)infection in vitro,and vice versa(Dejnirattisai et al.,2016;Stettler et al.,2016).The characteristics of enhancing antibodies,however,remain elusive.Some have suggested that non-neutralizing antibodies or antibodies recognizing specific antigenic sites are | Min Li Xin Wang Qihui Wang Lei Yu Lanfeng Wang Jinghua Yan Fuchun Zhang Linqi Zhang George F.Gao Xia Jin | 2017 | Science China(Life Sciences)2017，60，12： | 2 |
| 4 | Engineering topography: Effects on corneal cell behavior and integration into corneal tissue engineering显示文摘Cell-material interactions are important to tissue engineering.Inspired by the natural topographic structures on the extracellular matrix,a growing number of studies have integrated engineering topography into investigations of cell behavior on biomaterials.Engineering topography has a significant influence on cell behaviors.These cell-topography interactions play an important role in regenerative medicine and tissue engineering.Similarly,cell-topography interactions are important to corneal reconstruction and regeneration.In this review,we primarily summarized the effects of topographic cues on the behaviors of corneal cells,including cell morphology,adhesion,migration,and proliferation.Furthermore,the integration of engineering surface topography into corneal tissue engineering was also discussed. | Sijia Xiong HuiChang Gao Lanfeng Qin Yong-Guang Jia Li Ren | 2019 | Bioactive Materials2019，4，1： | 2 |
| 5 | Use of a high thoracic epidural analgesia for treatment of end-stage congestive heart failure secondary to coronary artery disease显示文摘 | Wu Shuang Fu Shiying Liu Fengqi Qu Renhai Wang Lanfeng Li Zhuqin Wang Xu | 2007 | International Journal of Cardiology2007，，2： | 1 |
| 6 | Crystal structures of NAC domains of human nascent polypeptide-associated complex (NAC) and its αNAC subunit显示文摘Nascent polypeptide associated complex(NAC)and its two isolated subunits,αNAC and βNAC,play important roles in nascent peptide targeting.We determined a 1.9Åresolution crystal structure of the interaction core of NAC heterodimer and a 2.4Åresolution crystal structure ofαNAC NAC domain homodimer.These structures provide detailed information of NAC heterodimerization and αNAC homodimerization.We found that the NAC domains of αNAC and βNAC share very similar folding despite of their relative low identity of amino acid sequences.Furthermore,different electric charge distributions of the two subunits at the NAC interface provide an explanation to the observation that the heterodimer of NAC complex is more stable than the single subunit homodimer.In addition,we successfully built a βNAC NAC domain homodimer model based on homologous modeling,suggesting that NAC domain dimerization is a general property of the NAC family.These 3D structures allow further studies on structurefunction relationship of NAC. | Lanfeng Wang Wenchi Zhang Lu Wang Xuejun C.Zhang Xuemei Li Zihe Rao | 2010 | Protein & Cell2010，1，4： | 1 |
| 7 | High Jc Epitaxial Superconducting YBa_2Cu_3O_(7-x) Thin Films显示文摘Excellent epitaxial growth of supercon-ducting YBa2 Cu3 O7-x thin films have beenrealized on(100)SrTiO3 and(100)ZrO2substrates by a planar rf or DC-magnetronsputtering apparatus with UHV system.Thequality of growth and the epitaxial orientationof the film strongly depended on substratetemperature,the substrate orientation and ox-ygen partial pressure.The films exhibitedsuperconducting onset at 92K and zero resist-ance at 90K with critical current density | Chen Lanfeng Zhou Li Wang Chaoqun General Research Institute of Non-ferrous Metals.Beijing,China Li Lin Zhao Bairu Zhang Yinzi Qiu Xianggang Institute of Physics,Academia Sinica,Beijing.China Pang Shijing Beijing Laboratory of Vacuum Physics,China Kou Yuanheng Department of Physics.Peking University,Beijing,China | 1990 | Rare Metals1990，10，1： | 0 |
| 8 | Intensive Observation of Reservoirinduced Seismicity and Preliminary Analysis on the Causes of Earthquakes in the Three Gorges Reservoir Area,Yangtze River显示文摘根据第 11 五年的计划的国家关键技术 RD 节目的要求,由 26 个地震车站组成的一个使增加密度的地震网络在 2009 年 3 月 21 短时期的地震计在湖北省的节在三个峡水库区域被建立, 5 宽带数字地震计。从三月到 2009 年 12 月, 2,995 ML -0 的一个总数。8 - 2。9 地震在从 145 m 三朵峡水库水水平玫瑰扣押到 172.8m 的试用期间被检测。用双差别地震地点算法, 2,837 地震精确被重定位。小本地地震的模式聚集在三个峡水库区域的结果表演呈现了线性分布或像团的簇分发,事件的像团的簇通常在从水滨的 5 km 的距离以内,并且地震的线性分发能被扩大到 16 km 的距离离开水滨。在三座峡水库的湖北节,地震主要在 Xiangxihe 河附近在 Xiannvshan 和 Jiuwanxi 差错的北结束被集中,并且沿着在在 Badong 区域的北河岸上的 Xietan 镇区和 Shenlongxi 区域的西方的长江的河岸,与焦点的深度不到 10km ,和 4km 在平均。在水库区域的地震频率与水库水水平变化有积极关联,显示 seismicity 属于水库导致的地震。沿着在水库区域的 Shenlong 河,地震在北 Badong 县显示出三线性分布,并且根据石灰岩地区常见的地形散布了分发。在碳酸盐阶层有地下的河。当水库被扣押时,水渗入了进地下的河,因此导致地震。在 Xiannvshan 的 crossriver 片断上的区域的地震指责, Jiuwanxi 差错并且在 Xietan , Shazhen 和 Xizhen 的区域西方,可能与弄软断绝有关 Xiannvshan 差错,或床上用品例如 Nukou 差错,连接,它将导致岩石群众的失败因此,导致地震。然而,关于被触发的机制的有说服力的结论仍然需要进一步的学习。另外, Zigui 县并且在 Badong 县的 Rangkou 镇东方的 Wenhua 和 Yanglin 向南接近区域, mininginduced 地震在水库的岸是的附近,并且上的矿发生了一些倒塌地震。 | Ma Wentao Xu Changpeng Yuan Jingli Li Hai'ou Xu Xiwei Zhang Xindong Zhang Lanfeng | 2011 | Earthquake Research in China2011，25，3： | 0 |
| 9 | Control Effect of UAV Low Volume Spray Technology on Thrips and Tomato Spotted Wilt Virus in Tobacco Field显示文摘[Objectives]The paper was to explore the influence of unmanned aerial vehicle(UAV)low volume spray technology on the control effect of viruliferous thrips and tomato spotted wilt virus(TSWV)under different pesticide concentrations,and to explore the effective control nodes and methods of thrips and TSWV.[Methods]According to the occurrence characteristics of thrips and TSWV,the field control effects of 4 pesticides on thrips and TSWV were tested by UAV with the thrips number and disease index as indicators,following the principle of regional and periodic control trials.[Results]In the groups of UAV and artificial prevention and control,4 agents were used alternatively,including 1%emamectin benzoate ME,1.7%abamectin imidacloprid ME,5%emamectin benzoate WDG,and 30%pyriproxyfen dinotefuran SL.There was no significant difference in the control effect of thrips and TSWV among treatment 1(recommended dosage of UAV agent),treatment 2(reduction of recommended dosage of UAV agent by 25%)and treatment 4(recommended dosage of artificial control agent),and the control effects on thrips were more than 83.16%in the 3 treatments.The disease index of TSWV in the 3 treatments decreased from 8.64±1.37 in the blank control group to less than 3,which reached the prevention and control threshold.But treatment 3(reduction of recommended dosage of UAV agent by 50%)did not reach the prevention and control threshold.The disease index of TSWV in the blank control area increased with the increase of the number of live nymphs of thrips,and there was a significant positive correlation,with good fitness.[Conclusions]UAV has a significant impact on the control effect of thrips and TSWV under different pesticide concentrations.In the actual flight control application,it is recommended that the amount of the pesticides is about 3/4 and not less than 1/2 of that of the artificial application.The control area should be extended to 100 m of the ridge of the tobacco field,and UAV is used periodically to control thrips and TSWV for 5 times from pre-transplanting stage to pre-squaring stage of flue-cured tobacco.The control effect is significantly different from the traditional artificial application. | Zefan LIU Dean XIE Jiabao HAN Yanfen XIE Li MA Siyuan GAO Ruiqi PENG Yuting ZHAO Xiangze FU Wengang CHENG Yonghong FENG Yan ZHANG Huaping ZHANG Ying HUANG Leijie DAI Lanfeng WANG | 2023 | Plant Diseases and Pests2023，14，4： | 0 |
| 10 | A human monoclonal antibody neutralizes SARS-CoV-2 Omicron variants bytargeting the upstream region of spikeprotein HR2 motif显示文摘The continuous emergence of new severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)variants meansthere is a need to explore additional strategies to develop broad-spectrum vaccines or therapeutics for individuals remaining at risk of coronavirus disease 2019(COVID-19).Neutralizing monoclonal antibody(mAb)that binds to theconserved S2 subunit of the SARS-CoV-2 spike(S)protein alone,or in combination with mAb that binds to the receptor-binding domain(RBD)of S protein,might be effective in eliciting protection from infection by a variety of SARS-CoV2 variants.Using high-throughput single-cell immunoglobulin sequencing of B cells from COVID-19-convalescent donors,we identified a high-affinity S2-specific mAb-39,that could inhibit original SARS-CoV-2 strain,Omicron BA.1,BA.2.86,BA.4,BA.5,and EG.5.1 S protein-mediated membrane fusion,leading to the neutralization of these pseudoviralinfections.Moreover,mAb-39 could also improve the neutralizing activity of anti-RBD antibody against the highlyneutralization-resistant Omicron variants.Molecular docking and point mutation analyses revealed that mAb-39 recognized epitopes within the conserved upstream region of the heptad repeat 2(HR2)motif of the S2 subunit.Collectively,these findings demonstrate that targeting the conserved upstream region of the HR2 motif(e.g.,using mAbs)provides anovel strategy for preventing the infection of SARS-CoV-2 and its variants. | Hang Su Jun Zhang Zhenfei Yi Sajid Khan Mian Peng Liang Ye Alan Bao Han Zhang Guangli Suo Qian Li Housheng Zheng Dandan Wu Thomas J.Kipps Lanfeng Wang Zhenghong Lin Suping Zhang | 2024 | hLife2024，2，3： | 0 |
| 11 | The Physalis floridana genome provides insights into the biochemical and morphological evolution of Physalis fruits显示文摘The fruits of Physalis(Solanaceae)have a unique structure,a lantern-like fruiting calyx known as inflated calyx syndrome(ICS)or the Chinese lantern,and are rich in steroid-related compounds.However,the genetic variations underlying the origin of these characteristic traits and diversity in Physalis remain largely unknown.Here,we present a high-quality chromosome-level reference genome assembly of Physalis floridana(~1.40Gb in size)with a contig N50 of~4.87Mb.Through evolutionary genomics and experimental approaches,we found that the loss of the SEP-like MADS-box gene MBP21 subclade is likely a key mutation that,together with the previously revealed mutation affecting floral MPF2 expression,might have contributed to the origination of ICS in Physaleae,suggesting that the origination of a morphological novelty may have resulted from an evolutionary scenario in which one mutation compensated for another deleterious mutation.Moreover,the significant expansion of squalene epoxidase genes is potentially associated with the natural variation of steroid-related compounds in Physalis fruits.The results reveal the importance of gene gains(duplication)and/or subsequent losses as genetic bases of the evolution of distinct fruit traits,and the data serve as a valuable resource for the evolutionary genetics and breeding of solanaceous crops. | Jiangjie Lu Meifang Luo Li Wang Kunpeng Li Yongyi Yu Weifei Yang Pichang Gong Huihui Gao Qiaoru Li Jing Zhao Lanfeng Wu Mingshu Zhang Xueyang Liu Xuemei Zhang Xian Zhang Jieyu Kang Tongyuan Yu Zhimin Li Yuannian Jiao Huizhong Wang Chaoying He | 2021 | Horticulture Research2021，8，1： | 0 |
| 12 | A 17–26.5 GHz 42.5 dBm broadband and highly efficient gallium nitride power amplifier design显示文摘A gallium nitride(GaN)power amplifier monolithic microwave integrated circuit(MMIC)with a wide band and high efficiency in the microwave frequency band is proposed in this study.The power amplifier MMIC uses a 0.15^im GaN high electron mo-bility transistor(HEMT)process.The operating frequency band of the amplifier can cover the whole K-band,i.e.,17-26.5 GHz. | Ming LI Zhiqun LI Quan ZHENG Lanfeng LIN Hongqi TAO | 2022 | Frontiers of Information Technology & Electronic Engineering2022，23，2： | 0 |
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