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| 1 | Topographic species-habitat associations of tree species in a heterogeneous tropical karst seasonal rain forest, China显示文摘Aims Tropical and subtropical karst forests of south China are under increas-ing pressure from over-exploitation causing widespread habitat degra-dation and biodiversity loss.Previous research has demonstrated that topography,as a proxy for resource availability,plays an important role in shaping tree species distributions in tropical forests.However,the association between growth stages and habitats types has not been considered in this analysis.Our aim was to examine the differences among different habitat types to determine whether tree species show similar species-habitat associations at young and mature life stages.Methods We used multivariate regression tree analysis to examined species-habitat associations among eight topographically defined habitats.The results were tested with a torus-translation test and canonical correspondence analysis(CCA)for 74 species in a 15 ha karst tropi-cal seasonal rain forest at the Nonggang National Natural Reserve in south China.We considered two life stages(young and mature)of trees species that showed a positive association with topography.Important Findings We found marked differences in community characteristics and number of associations among the eight habitats.Of the 74 species subjected to torus-translation test,63 had significant positive and 70 had significant negative associations with one or more of the eight habitats.Positive associations were more frequent in higher elevation habitats and negative associations were more frequent in lower eleva-tion habitats.This suggests that edaphic and hydrological variation related to topography play important roles in habitat partitioning in heterogeneous karst forests.For the 63 tree species with significant positive associations to at least one habitat,40 of them had the same positive association at young and mature life stages.The CCA revealed that the six topographic variables considered had consistent relation-ships with species distribution among all individuals and their two life stages.This indicates that most of the karst forest tree species show consistent associations with a single habitat throughout their life.We conclude that niche differentiation plays an important role in main-taining the diversity of this heterogeneous species-rich karst forest. | Yili Guo Bin Wang Azim U.Mallik Fuzhao Huang Wusheng Xiang Tao Ding Shujun Wen Shuhua Lu Dongxing Li Yunlin He Xiankun Li | 2017 | Journal of Plant Ecology2017，10，3： | 6 |
| 2 | An optimized protocol for stepwise optimization of real-time RT-PCR analysis显示文摘Computational tool-assisted primer design for real-time reverse transcription(RT)PCR(qPCR)analysis largely ignores the sequence similarities between sequences of homologous genes in a plant genome.It can lead to false confidence in the quality of the designed primers,which sometimes results in skipping the optimization steps for qPCR.However,the optimization of qPCR parameters plays an essential role in the efficiency,specificity,and sensitivity of each gene’s primers.Here,we proposed an optimized approach to sequentially optimizing primer sequences,annealing temperatures,primer concentrations,and cDNA concentration range for each reference(and target)gene.Our approach started with a sequence-specific primer design that should be based on the single-nucleotide polymorphisms(SNPs)present in all the homologous sequences for each of the reference(and target)genes under study.By combining the efficiency calibrated and standard curve methods with the 2−ΔΔCt method,the standard cDNA concentration curve with a logarithmic scale was obtained for each primer pair for each gene.As a result,an R 2≥0.9999 and the efficiency(E)=100±5% should be achieved for the best primer pair of each gene,which serve as the prerequisite for using the 2^(−ΔΔCt) method for data analysis.We applied our newly developed approach to identify the best reference genes in different tissues and at various inflorescence developmental stages of Tripidium ravennae,an ornamental and biomass grass,and validated their utility under varying abiotic stress conditions.We also applied this approach to test the expression stability of six reference genes in soybean under biotic stress treatment with Xanthomonas axonopodis pv.glycines(Xag).Thus,these case studies demonstrated the effectiveness of our optimized protocol for qPCR analysis. | Fangzhou Zhao Nathan A.Maren Pawel Z.Kosentka Ying-Yu Liao Hongyan Lu James R.Duduit Debao Huang Hamid Ashrafi Tuanjie Zhao Alejandra I.Huerta Thomas G.Ranney Wusheng Liu | 2021 | Horticulture Research2021，8，1： | 2 |
| 3 | Low-complexity and energy efficient image compression scheme for wireless sen- sor networks显示文摘 | Lu Qin Luo Wusheng Wang Jidong | 2008 | Computer Networks2008，52，13： | 1 |
| 4 | Filter-based methodology for the location of hot spots in proteins and Exons in DNA显示文摘 | RAMACHANDRAN P LU WUSHENG ANTONIOU A | 2012 | IEEE Transactions on Biomedical Engineering2012，59，6： | 1 |
| 5 | Reconstruction of sparse signal by minimizing a reweighted approximate l0-norm in the null space of the measurement matrix 显示文摘 | Pant J K Lu Wusheng Antoniou A | 2005 | IEEE Transactions on Signal Procssing2005，53，8： | 1 |
| 6 | Design of underground sonde of a directional drilling locator system显示文摘 | Xu Tao Luo Wusheng Lu Haibao | 2005 | Sensor and Actuators A: Phys2005，，2： | 1 |
| 7 | Design of practically perfect reconstruction cosine modulated filter banks: a second-order cone pro- gramming approach 显示文摘 | Lu Wusheng | 2004 | IEEE Transactions on Circuits and Systems2004，51，3： | 1 |
| 8 | An improved weighted least squares design for variable fractional delay FIR filters 显示文摘 | LU Wusheng DENG Tianbo | 1999 | IEEE Trans Circuits and Systems-II: Analog and Digital Signal Processing1999，46，8： | 1 |
| 9 | Linear interpolation in pilot symbol assited channel estimation for OFDM显示文摘 | Dong Xiaodai Lu Wusheng Song C K | 2007 | IEEE Trans on Wireless Communications2007，6，5： | 1 |
| 10 | Low-complexity and energy efficient image compression scheme for wireless sensor networks显示文摘 | Lu Qin Luo Wusheng Wang Jidong | 2008 | Computer Networks2008，52，13： | 1 |
| 11 | Efficient Iterative Design Method for Cosine-modulated QMF Banks显示文摘 | Xu Hua Lu Wusheng Andreas A | 1996 | IEEE Trans on Signal Processing1996，44，7： | 1 |
| 12 | Efficient iterative design method for cosine-modulated QMF banks 显示文摘 | Xu Hua Lu Wusheng Andreas A | 1996 | IEEE Transactions on Signal Processing1996，44，7： | 1 |
| 13 | Image denoising by generalized total variation regularization and least squares fidelity 显示文摘 | Y AN Jie Lu Wusheng | 2013 | Multidimensional Systems and Signal Processing2013，，： | 1 |
| 14 | MIMO relaying broadcast channels with linear precoding and quantized channel state information feedback显示文摘 | Xu Wei Dong Xiaodai Lu Wusheng | 2010 | IEEE Trans on Signal Processing2010，58，10： | 1 |
| 15 | Reconstruc- tion of sparse signal by minimizing a re - weighted approxi- mate 10 - norm in the null space of the measurement matrix 显示文摘 | Jeevan K Pant Lu Wusheng Andreas Antoniou | 2005 | IEEE Transactions on Signal Procssing2005，53，8： | 1 |
| 16 | MIMO re- laying broadcast channels with linear precoding and quantized channel state information feedback 显示文摘 | Xu Wei DONG Xiaodai Lu Wusheng | 2010 | IEEE Transactions on signal processing2010，58，10： | 1 |
| 17 | Conversion resection for patients with hepatocellular carcinoma and inferior vena cava tumor thrombus: a consecutive case series显示文摘To the Editor:The incidence of hepatocellular carcinoma(HCC)with inferior vena cava tumor thrombus(IVCTT)is.approximately 1.4%.Owing to direct cancercll dissemination into the systemic circulation,HCC-IVCTT is frequently associated with synchronous and metachronous extrahepatic metastasis.Without treatment,the median survival time is only 3 months for patients with HCC-IVCTT. | Xiaoyun Zhang Wei Peng Chuan Li Wusheng Lu Zhiping Li Jiayin Yang Tianfu Wen | 2022 | Chinese Medical Journal2022，，12： | 1 |
| 18 | Design of underground sonde of a directional drilling locator system 显示文摘 | Xu Tao Luo Wusheng Lu Haibao | 2005 | Sensors and Actu- ators A2005，119，： | 1 |
| 19 | A robust photoelectric angular po- sition sensor especially for a steerable underground boring tool显示文摘 | Xu Tao Lu Haibao Luo Wusheng | 2005 | Sensors and Actuators A2005，120，： | 1 |
| 20 | Engineered Cleistogamy in Camelina sativa for bioconfinement显示文摘Camelina sativa is a self-pollinating and facultative outcrossing oilseed crop.Genetic engineering has been used to improve camelina yield potential for altered fatty acid composition,modified protein profiles,improved seed and oil yield,and enhanced drought resistance.The deployment of transgenic camelina in the field posits high risks related to the introgression of transgenes into nontransgenic camelina and wild relatives.Thus,effective bioconfinement strategies need to be developed to prevent pollen-mediated gene f low(PMGF)from transgenic camelina.In the present study,we overexpressed the cleistogamy(i.e.f loral petal non-openness)-inducing PpJAZ1 gene from peach in transgenic camelina.Transgenic camelina overexpressing PpJAZ1 showed three levels of cleistogamy,affected pollen germination rates after anthesis but not during anthesis,and caused a minor silicle abortion only on the main branches.We also conducted field trials to examine the effects of the overexpressed PpJAZ1 on PMGF in the field,and found that the overexpressed PpJAZ1 dramatically inhibited PMGF from transgenic camelina to non-transgenic camelina under the field conditions.Thus,the engineered cleistogamy using the overexpressed PpJAZ1 is a highly effective bioconfinement strategy to limit PMGF from transgenic camelina,and could be used for bioconfinement in other dicot species. | Debao Huang Liwei Gao Jeremy McAdams Fangzhou Zhao Hongyan Lu Yonghui Wu Jeremy Martin Sherif MSherif Jayasankar Subramanian Hui Duan Wusheng Liu | 2023 | Horticulture Research2023，10，2： | 0 |
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