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| 1 | Mapping QTLs with epistatic effects and QTL×environment interactions for plant height using a doubled haploid population in cultivated wheat显示文摘Quantitative trait loci (QTLs) for plant height in wheat (Triticum aestivum L.) were studied using a set of 168 doubled haploid (DH) lines, which were derived from the cross Huapei 3/Yumai 57. A genetic linkage map was constructed using 283 SSR and 22 EST-SSR markers. The DH population and the parents were evaluated for wheat plant height in 2005 and 2006 in Tai’an and 2006 in Suzhou. QTL analyses were performed using the software of QTLNetwork version 2.0 based on the mixed linear model. Four additive QTLs and five pairs of epistatic effects were detected, which were distributed on chromosomes 3A, 4B, 4D, 5A, 6A, 7B, and 7D. Among them, three additive QTLs and three pairs of epistatic QTLs showed QTL×environment interactions (QEs). Two major QTLs, Qph4B and Qph4D, which accounted for 14.51% and 20.22% of the phenotypic variation, were located similar to the reported locations of the dwarfing genes Rht1 and Rht2, respectively. The Qph3A-2 with additive effect was not reported in previous linkage mapping studies. The total QTL effects detected for the plant height explained 85.04% of the phenotypic variation, with additive effects 46.07%, epistatic effects 19.89%, and QEs 19.09%. The results showed that both additive effects and epistatic effects were important genetic bases of wheat plant height, which were subjected to environmental modifications, and caused dramatic changes in phenotypic effects. The information obtained in this study will be useful for manipulating the QTLs for wheat plant height by molecular marker-assisted selection (MAS). | Kunpu Zhang Jichun Tian Liang Zhao Shanshan Wang | 2008 | Journal of Genetics and Genomics2008，35，2： | 36 |
| 2 | The PI3K/AKT Pathway and Renal Cell Carcinoma显示文摘The phosphatidylinositol 3 kinase(PI3K)/AKT pathway is genetically targeted in more pathway components and in more tumor types than any other growth factor signaling pathway,and thus is frequently activated as a cancer driver.More importantly,the PI3K/AKT pathway is composed of multiple bifurcating and converging kinase cascades,providing many potential targets for cancer therapy.Renal cell carcinoma(RCC) is a high-risk and high-mortality cancer that is notoriously resistant to traditional chemotherapies or radiotherapies.The PI3K/AKT pathway is modestly mutated but highly activated in RCC,representing a promising drug target.Indeed,PI3 K pathway inhibitors of the rapalog family are approved for use in RCC.Recent large-scale integrated analyses of a large number of patients have provided a molecular basis for RCC,reiterating the critical role of the PI3K/AKT pathway in this cancer.In this review,we summarize the genetic alterations of the PI3K/AKT pathway in RCC as indicated in the latest large-scale genome sequencing data,as well as treatments for RCC that target the aberrant activated PI3K/AKT pathway. | Huifang Guo Peter German Shanshan Bai Sean Barnes Wei Guo Xiangjie Qi Hongxiang Lou Jiyong Liang Eric Jonasch Gordon B.Mills Zhiyong Ding | 2015 | Journal of Genetics and Genomics2015，42，7： | 39 |
| 3 | Suppression of Jasmonic Acid-Mediated Defense by Viral-Inducible MicroRNA319 Facilitates Virus Infection in Rice显示文摘MicroRNAs (miRNAs ) 是植物开发和主人病毒相互作用的枢轴的调节的人。然而,角色和涉及病毒的感染和主人危险性的特定的 miRNAs 的行动模式仍然保持大部分不清楚。在这研究,我们给那瑞斯看引起 miR319 的增加的累积,但是减少的破旧绝技病毒(RRSV ) 感染调整 miR319 的 TCP (TEOSINTE BRANCHED/CYCLOIDEA/PCF ) 的表示基因,特别 TCP21,在米饭植物。转基因的米饭植物 overexpressing miR319 或 downregulating TCP21 展出了像疾病的显型并且与野类型的植物比较显示出显著地更高的危险性到 RRSV。相反,仅仅温和的疾病症状在感染 RRSV 的线 overexpressing TCP21 并且特别在转基因的植物 overexpressing 被观察 miR319 抵抗的 TCP21。miR319 的 RRSV 感染和 overexpression 在米饭与 JA 生合成和信号相关的基因的 downregulated 表示一起引起了减少的内长的 jasmonic 酸(JA ) 层次。然而,有甲基 jasmonate 的米饭植物的治疗减轻了 RRSV 引起并且减少了病毒累积的疾病症状。一起拿,我们的结果建议由在米饭的 RRSV 感染的 miR319 的正式就职压制调停 JA 的防卫便于病毒感染和症状开发。 | Chao Zhang Zuomei Ding Kangcheng Wu Liang Yang Yang Li Zhen Yang Shan Shi Xiaojuan Liu Shanshan Zhao Zhirui Yang Yu Wang Luping Zheng Juan Wei Zhenguo Du Aihong Zhang Hongqin Miao Yi Li Zujian Wu Jianguo Wu | 2016 | Molecular Plant2016，9，9： | 27 |
| 4 | Minimal invasive microscopic tooth preparation in esthetic restoration: a specialist consensus显示文摘By removing a part of the structure,the tooth preparation provides restorative space,bonding surface,and finish line for various restorations on abutment.Preparation technique plays critical role in achieving the optimal result of tooth preparation.With successful application of microscope in endodontics for>30 years,there is a full expectation of microscopic dentistry.However,as relatively little progress has been made in the application of microscopic dentistry in prosthodontics,the following assumptions have been proposed:Is it suitable to choose the tooth preparation technique under the naked eye in the microscopic vision?Is there a more accurate preparation technology intended for the microscope?To obtain long-term stable therapeutic effects,is it much easier to achieve maximum tooth preservation and retinal protection and maintain periodontal tissue and oral function health under microscopic vision?Whether the microscopic prosthodontics is a gimmick or a breakthrough in obtaining an ideal tooth preparation should be resolved in microscopic tooth preparation.This article attempts to illustrate the concept,core elements,and indications of microscopic minimally invasive tooth preparation,physiological basis of dental pulp,periodontium and functions involved in tool preparation,position ergonomics and visual basis for dentists,comparison of tooth preparation by naked eyes and a microscope,and comparison of different designs of microscopic minimally invasive tooth preparation techniques.Furthermore,a clinical protocol for microscopic minimally invasive tooth preparation based on target restorative space guide plate has been put forward and new insights on the quantity and shape of microscopic minimally invasive tooth preparation has been provided. | Haiyang Yu Yuwei Zhao Junying Li Tian Luo Jing Gao Hongchen Liu Weicai Liu Feng Liu Ke Zhao Fei Liu Chufan Ma Juergen MSetz Shanshan Liang Lin Fan Shanshan Gao Zhuoli Zhu Jiefei Shen Jian Wang Zhimin Zhu Xuedong Zhou | 2019 | International Journal of Oral Science2019，11，4： | 25 |
| 5 | Sleep Deprivation Selectively Down-Regulates Astrocytic 5-HT2B Receptors and Triggers Depressive-Like Behaviors via Stimulating P2X7 Receptors in Mice显示文摘Chronic loss of sleep damages health and disturbs the quality of life.Long-lasting sleep deprivation(SD)as well as sleep abnormalities are substantial risk factors for major depressive disorder,although the underlying mechanisms are not clear.Here,we showed that chronic SD in mice promotes a gradual elevation of extracellular ATP,which activates astroglial P2X7 receptors(P2X7Rs).Activated P2X7Rs,in turn,selectively down-regulated the expression of 5-HT2B receptors(5-HT2BRs)in astrocytes.Stimulation of P2X7Rs induced by SD selectively suppressed the phosphorylation of AKT and FoxO3 a in astrocytes,but not in neurons.The overexpression of FoxO3a in astrocytes inhibited the expression of 5-HT2BRs.Down-regulation of 5-HT2BsRs instigated by SD suppressed the activation of STAT3 and relieved the inhibition of Ca2+-dependent phospholipase A2.This latter cascade promoted the release of arachidonic acid and prostaglandin E2.The depression-like behaviors induced by SD were alleviated in P2X7R-KO mice.Our study reveals the mechanism underlying chronic SD-induced depression-like behaviors and suggests 5-HT2BRs as a key target for exploring therapeutic strategies aimed at the depression evoked by sleep disorders. | Maosheng Xia Zexiong Li Shuai Li Shanshan Liang Xiaowei Li Beina Chen Manman Zhang Chengyi Dong Alexei Verkhratsky Dawei Guan Baoman Li | 2020 | Neuroscience Bulletin2020，36，11： | 10 |
| 6 | Single-cell transcriptomic atlas of primate cardiopulmonary aging显示文摘Aging is a major risk factor for many diseases,especially in highly prevalent cardiopulmonary comorbidities and infectious diseases including Coronavirus Disease 2019(COVID-19).Resolving cellular and molecular mechanisms associated with aging in higher mammals is therefore urgently needed.Here,we created young and old non-human primate single-nucleus/cell transcriptomic atlases of lung,heart and artery,the top tissues targeted by SARS-CoV-2.Analysis of cell type-specific aging-associated transcriptional changes revealed increased systemic inflammation and compromised virus defense as a hallmark of cardiopulmonary aging.With age,expression of the SARS-CoV-2 receptor angiotensin-converting enzyme 2(ACE2)was increased in the pulmonary alveolar epithelial barrier,cardiomyocytes,and vascular endothelial cells.We found that interleukin 7(IL7)accumulated in aged cardiopulmonary tissues and induced ACE2 expression in human vascular endothelial cells in an NF-κB-dependent manner.Furthermore,treatment with vitamin C blocked IL7-induced ACE2 expression.Altogether,our findings depict the first transcriptomic atlas of the aged primate cardiopulmonary system and provide vital insights into age-linked susceptibility to SARS-CoV-2,suggesting that geroprotective strategies may reduce COVID-19 severity in the elderly. | Shuai Ma Shuhui Sun Jiaming Li Yanling Fan Jing Qu Liang Sun Si Wang Yiyuan Zhang Shanshan Yang Zunpeng Liu Zeming Wu Sheng Zhang Qiaoran Wang Aihua Zheng Shuguang Duo Yang Yu Juan Carlos Izpisua Belmonte Piu Chan Qi Zhou Moshi Song Weiqi Zhang Guang-Hui Liu | 2021 | Cell Research2021，31，4： | 10 |
| 7 | Development and validation of an endoscopic images-based deep learning model for detection with nasopharyngeal malignancies显示文摘Background:Due to the occult anatomic location of the nasopharynx and frequent presence of adenoid hyperpla-sia,the positive rate for malignancy identification during biopsy is low,thus leading to delayed or missed diagnosis for nasopharyngeal malignancies upon initial attempt.Here,we aimed to develop an artificial intelligence tool to detect nasopharyngeal malignancies under endoscopic examination based on deep learning.Methods:An endoscopic images-based nasopharyngeal malignancy detection model(eNPM-DM)consisting of a fully convolutional network based on the inception architecture was developed and fine-tuned using separate training and validation sets for both classification and segmentation.Briefly,a total of 28,966 qualified images were collected.Among these images,27,536 biopsy-proven images from 7951 individuals obtained from January 1st,2008,to December 31st,2016,were split into the training,validation and test sets at a ratio of 7:1:2 using simple randomiza-tion.Additionally,1430 images obtained from January 1st,2017,to March 31st,2017,were used as a prospective test set to compare the performance of the established model against oncologist evaluation.The dice similarity coef-ficient(DSC)was used to evaluate the efficiency of eNPM-DM in automatic segmentation of malignant area from the background of nasopharyngeal endoscopic images,by comparing automatic segmentation with manual segmenta-tion performed by the experts.Results:All images were histopathologically confirmed,and included 5713(19.7%)normal control,19,107(66.0%)nasopharyngeal carcinoma(NPC),335(1.2%)NPC and 3811(13.2%)benign diseases.The eNPM-DM attained an overall accuracy of 88.7%(95%confidence interval(CI)87.8%-89.5%)in detecting malignancies in the test set.In the prospective comparison phase,eNPM-DM outperformed the experts:the overall accuracy was 88.0%(95%CI 86.1%-89.6%)vs.80.5%(95%CI 77.0%-84.0%).The eNPM-DM required less time(40 s vs.110.0±5.8 min)and exhibited encouraging performance in automatic segmentation of nasopharyngeal malignant area from the background,with an average DSC of 0.78±0.24 and 0.75±0.26 in the test and prospective test sets,respectively.Conclusions:The eNPM-DM outperformed oncologist evaluation in diagnostic classification of nasopharyngeal mass into benign versus malignant,and realized automatic segmentation of malignant area from the background of nasopharyngeal endoscopic images. | Chaofeng Li Bingzhong Jing Liangru Ke Bin Li Weixiong Xia Caisheng He Chaonan Qian Chong Zhao Haiqiang Mai Mingyuan Chen Kajia Cao Haoyuan Mo Ling Guo Qiuyan Chen Linquan Tang Wenze Qiu Yahui Yu Hu Liang Xinjun Huang Guoying Liu Wangzhong Li Lin Wang Rui Sun Xiong Zou Shanshan Guo Peiyu Huang Donghua Luo Fang Qiu Yishan Wu Yijun Hua Kuiyuan Liu Shuhui Lv Jingjing Miao Yanqun Xiang Ying Sun Xiang Guo Xing Lv | 2018 | Cancer Communications2018，38，1： | 9 |
| 8 | Famitinib in combination with concurrent chemoradiotherapy in patients with locoregionally advanced nasopharyngeal carcinoma: a phase 1, open-label, dose-escalation Study显示文摘Background:Famitinib is a tyrosine kinase inhibitor against multiple targets,including vascular endothelial growth factor receptor 2/3,platelet-derived growth factor receptor,and stem cell factor receptor(c-kit).Previous studies have demonstrated anti-tumour activities of famitinib against a wide variety of advanced-stage solid cancers.We aimed to determine the safety and efficacy of famitinib with concurrent chemoradiotherapy(CCRT)in patients with locoregionally advanced nasopharyngeal carcinoma(NPC).We also evaluated the feasibility of contrast-enhanced ultrasound(D-CEUS)as a predictor of early tumour response to famitinib and to correlate functional parameters with clinical efficacy.Methods:The trial was conducted in subjects with stage III or IVa-b NPC using a 3+3 design of escalating fami-tinib doses.Briefly,subjects received 2 weeks of famitinib monotherapy followed by 7 weeks of famitinib plus CCRT.D-CEUS of the neck lymph nodes was performed at day 0,8 and 15 after famitinib was administered before starting concurrent chemoradiotherapy.End points included safety,tolerability and anti-tumour activity.Results:Twenty patients were enrolled(six each for 12.5,16.5 and 20 mg and two for 25 mg).Two patients in the 25 mg cohort developed dose-limiting toxicities,including grade 4 thrombocytopenia and grade 3 hypertension.The most common grade 3/4 adverse events were leukopenia,neutropenia and radiation mucositis.D-CEUS tests showed that more than 60%of patients achieved a perfusion parameter response after 2 weeks taking famitinib alone,and the parameter response was associated with disease improvement.In the famitinib monotherapy stage,three patients(15%)showed partial responses.The complete response rate was 65%at the completion of treatment and 95%3 months after the treatment ended.After a median follow-up of 44 months,the 3-year progression-free survival(PFS)and distant metastasis-free survival were 70%and 75%,respectively.Subjects with a decrease of perfusion parameter response,such as peak intensity decreased at least 30%after 1 week of famitinib treatment,had higher 3-year PFS(90.9%vs.44.4%,95%CI 73.7%-100%vs.11.9%-76.9%,P<0.001)than those with an increase or a reduction of less than 30%.Conclusions:The recommended famitinib dose for phase II trial is 20 mg with CCRT for patients with local advanced NPC.D-CEUS is a reliable and early measure of efficacy for famitinib therapies.Further investigation is required to confirm the effects of famitinib plus chemoradiotherapy. | Qiuyan Chen Linquan Tang Na Liu Feng Han Ling Guo Shanshan Guo Jianwei Wang Huai Liu Yanfang Ye Lu Zhang Liting Liu Pan Wang Yingqin Li Qingmei He Xiaoqun Yang Qingnan Tang Yang Li YuJing Liang XueSong Sun Chuanmiao Xie Yunxian Mo Ying Guo Rui Sun Haoyuan Mo Kajia Cao Xiang Guo Musheng Zeng Haiqiang Mai Jun Ma | 2018 | Cancer Communications2018，38，1： | 6 |
| 9 | The most typical shape of oceanic mesoscale eddies from global satellite sea level observations显示文摘在这研究,我们由使用海水平异例( SLA )数据的卫星观察决定这正规化基于的海洋旋涡的最典型的形状与非线性的最佳的适合修改了分析功能使海洋旋涡的特征正常化最典型的旋涡是泰勒旋涡(50%),它展出 塑造Gaussian 的 exp ( r 2) SLA 和涡度分布(是的 1 r 2)exp(r 2)规范的半径r.的功能越大旋涡的振幅,旋涡越可能而且是Gaussian-shaped.,约40%海洋旋涡是有不同参数的二个 Gaussian 旋涡的联合,但是旋涡的这些类型的作文仅仅比 Gaussian 是象一个二次的旋涡一样的更多海洋的旋涡的小部分是纯二次的旋涡(<10%)与一样的涡度分发,作为一个 Rankine 旋涡,我们断定泰勒旋涡是典型形状的好近似海洋回旋。 | Zifei WANG Qiuyang LI Liang SUN Song LI Yuanjian YANG Shanshan LIU | 2015 | Frontiers of Earth Science2015，9，2： | 6 |
| 10 | Extensive profiling of the expressions of tRNAs and tRNA-derived fragments(tRFs)reveals the complexities of tRNA and tRF populations in plants显示文摘Evidence is emerging that t RNA-derived fragments(t RFs)are regulatory molecules.Studies of t RFs in plants have been based on conventional small RNA sequencing,and focused on profiling of t RF-5 and t RF-3 species.A more comprehensive and quantitative analysis of the entire t RF population is highly necessary.Here,we employ t RNA-seq and YAMAT-seq,and develop a bioinformatics tool to comprehensively profile the expressions of t RNAs and t RFs in plants.We show that in Arabidopsis,approximately half of t RNA genes are extremely weakly expressed,accounting for only 1%of total t RNA abundance,while~12%of t RNA genes contribute to~80%of t RNA abundance.Our t RNA sequencings in various plants reveal that t RNA expression profiles exhibit a cross-species conserved pattern.By characterizing the composition of a highly heterogeneous t RF population,we show that t RNA halves and previously unnoticed 10–16-nt tiny t RFs represent substantial portions.The highly accumulated 13-nt and 16-nt tiny t RFs in Arabidopsis indicate that tiny t RFs are not random t RNA degradation products.Finally,we provide a user-friendly database for displaying the dynamic spatiotemporal expressions of t RNAs and t RFs in the model plants Arabidopsis and rice. | Xuan Ma Chunyan Liu Xiaocong Kong Jing Liu Siju Zhang Shanshan Liang Weijiang Luan Xiaofeng Cao | 2021 | Science China(Life Sciences)2021，64，4： | 5 |
| 11 | Iron Aggravates the Depressive Phenotype of Stressed Mice by Compromising the Glymphatic System显示文摘Dear Editor,The pathophysiologic al mechanisms underlying mood disorders including major depressive disorder(MDD)remain to be fully characterized.Iron is a key component in the development of the central nervous system and iron deficiency has been linked to impairments of mood and cognition[1]. | Shanshan Liang Yan Lu Zexiong Li Shuai Li Beina Chen Manman Zhang Binjie Chen Ming Ji Wenliang Gong Maosheng Xia Alexei Verkhratsky Xu Wu Baoman Li | 2020 | Neuroscience Bulletin2020，36，12： | 5 |
| 12 | An Improvement to Multiple Criteria ABC Inventory Classification Using Shannon Entropy显示文摘This paper extends the Ng-model [Ng, 2007] for multiple criteria ABC inventory classification based upon Shannon entropy. The proposed approach determines the common weights associated with all criteria importance rankings, and provides a comprehensive scoring scheme by aggregating all rankings of the criteria importance. A numerical illustration is presented to compare the model with previous studies. | ZHENG Shanshan FU Yelin LAI Kin Keung LIANG Liang | 2017 | Journal of Systems Science & Complexity2017，30，4： | 4 |
| 13 | Silicon lithium-ion battery anode with enhanced performance: Multiple effects of silver nanoparticles显示文摘Silicon has been regarded as one of the most promising next generation lithium-ion battery anode. However, the poor cyclic stability of the Si based anode has severely limited its practical applications, which is even worse with high mass loading density(>1 mg cm^(-2)). A new concept has been developed to enhance the electrochemical performance of the Si nanoparticle anode. Silver nanoparticles are composited with the silicon nanoparticles in a facile way for the first time. It is found that the mechanical properties of the Si electrode have been significantly improved by the incorporation of the silver nanoparticles, leading to enhanced cyclic performance. With the Si/Ag mass ratio of 4:1, the reversible specific discharge capacity is retained as 1156 m A h g^(-1) after 100 cycles at 200 mA g^(-1), which is more than three times higher than that of the bare silicon(318 mA h g^(-1)). The rate performance has been effectively improved as well due to excellent electron conductivity of the silver nanoparticles. | Shanshan Yin Qing Jia Xiuxia Zuo Shuang Xie Kai Fang Yonggao Xia Jinlong Li Bao Qiu Meimei Wang Jianzhen Ban Xiaoyan Wang Yi Zhang Ying Xiao Luyao Zheng Suzhe Liang Zhaoping Liu Cundong Wang Ya-Jun Cheng | 2018 | Journal of Materials Science & Technology2018，34，10： | 4 |
| 14 | Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton's jelly in vitro显示文摘Seeding cells and scaffolds play pivotal roles in bone tissue engineering and regenerative medicine.Wharton’s jelly-derived mesenchymal stem cells(WJCs)from human umbilical cord represent attractive and promising seeding cells in tissue regeneration and engineering for treatment applications.This study was carried out to explore the biocompatibility of scaffolds to seeding cells in vitro.Rod-like nano-hydroxyapatite(RN-HA)and flake-like micro-hydroxyapatite(FM-HA)coatings were prepared on Mg-Zn-Ca alloy substrates using micro-arc oxidation and electrochemical deposition.WJCs were utilized to investigate the cellular biocompatibility of Mg-Zn-Ca alloys after different surface modifications by observing the cell adhesion,morphology,proliferation,and osteoblastic differentiation.The in vitro results indicated that the RN-HA coating group was more suitable for cell proliferation and cell osteoblastic differentiation than the FM-HA group,demonstrating better biocompatibility.Our results suggested that the RN-HA coating on Mg-Zn-Ca alloy substrates might be of great potential in bone tissue engineering. | GUAN FangXia MA ShanShan SHI XinYi MA Xun CHI LianKai LIANG Shuo CUI YuanBo WANG ZhiBin YAO Ning GUAN ShaoKang YANG Bo | 2014 | Science China(Life Sciences)2014，57，2： | 4 |
| 15 | Prussian blue nanoparticle-loaded microbubbles for photothermally enhanced gene delivery through ultrasound-targeted microbubble destruction显示文摘By adsorbing chitosan(CS)-functionalized Prussian blue(PB) nanoparticles(CS/PB NPs) complexing DNA onto the surface of gas encapsulated microbubbles(MBs), a multifunctional gene delivery system of MBs@CS/PB/DNA was fabricated for photothermally enhanced gene transfection through ultrasound-targeted microbubble destruction. CS/PB NPs of(2.69 ± 0.49) nm could complex DNA effectively when the mass ratio was2:1. It was found that MBs@CS/PB/DNA could enhance ultrasound imaging greatly both in vitro and in vivo. In addition, MBs@CS/PB/DNA could be disrupted by applying a higher-intensity ultrasound irradiation to release CS/PB/DNA, which could effectively transform the nearinfrared(NIR) light into heat to assist the uptake of CS/PB/DNA by cells. With the aid of ultrasound irradiation and NIR light irradiation, the gene transfection efficiency was significantly enhanced to(43.08 ± 1.13) %, much higher than polyethylenimine. Moreover, MBs@CS/PB/DNA showed excellent biocompatibility, encouraging the further exploration of MBs@CS/PB/DNA to be a platform for combined ultrasound image, photothermal therapy, drug delivery, and gene therapy. | Xiaoda Li Xiuli Yue Jinrui Wang Xiaolong Liang Lijia Jing Li Lin Yongbo Yang Shanshan Feng Yajun Qian Zhifei Dai | 2016 | Science Bulletin2016，61，2： | 4 |
| 16 | Mapping Toll-Like Receptor Signaling Pathway Genes of Zhikong Scallop(Chlamys farreri) with FISH显示文摘Toll-like receptor(TLR) signaling pathway plays a pivotal role in the innate immune system. Studies on TLR signaling pathway genes in Zhikong scallop(Chlamys farreri) have mainly focused on sequence analysis and expression profiling, no research has been carried out on their localization. The chromosomal position of TLR signaling pathway genes can be valuable for assemblying scallop genome and analysizing gene regulatory networks. In the present study, five key TLR signaling pathway genes(Cf TLR, Cf Myd88, Cf TRAF6, Cf NFκB, and Cf IκB) containing bacterial artificial chromosomes(BACs) were isolated and physically mapped through fluorescence in situ hybridization on five non-homologous chromosome pairs, showing a similar distribution to another five model species. The isolation and mapping of these key immune genes of C. farreri will aid to the research on innate immunity, assignment of interested genes to chromosomes, and integration of physical, linkage and cytogenetic maps of this species. | ZHAO Bosong ZHAO Liang LIAO Huan CHENG Jie LIAN Shanshan LI Xuan HUANG Xiaoting BAO Zhenmin | 2015 | Journal of Ocean University of China2015，14，6： | 3 |
| 17 | Dynamic cell transition and immune response landscapes of axolotl limb regeneration revealed by single-cell analysis显示文摘Dear Editor,The axolotl,Ambystoma mexicanum,has extraordinary capability to fully recover multiple tissues after lost,whereas such capability has disappeared in mammals.Thus,deci-phering detailed mechanisms underlying axolotl regenera-tion could provide valuable lessons for regenerative medicine.However,many questions,such as the origin of essential progenitor cells and key responses of individual types of cells for regeneration remain elusive(Haas and Whited,2017).Newly developed single-cell RNA sequenc-ing(scRNA-seq)method enables researchers to observe cellular and molecular dynamics in axolotl regeneration at the single-cell resolution(Gerber et al.,2018;Leigh et al.,2018),but the reported transcriptome landscapes are only for certain cell types or in certain regenerative stages.A complete overview of the regeneration process for all cell types is still lacking. | Hanbo Li Xiaoyu Wei Li Zhou Weiqi Zhang Chen Wang Yang Guo Denghui Li Jjianyang Chen Tianbin Liu Yingying Zhang Shuai Ma Congyan Wang Fujian Tan Jiangshan Xu Yang Liu Yue Yuan Liang Chen Qiaoran Wang Jing Qu Yue Shen Shanshan Liu Guangyi Fan Longqi Liu Xin Liu Yong Hou Guang-Hui Liu Ying Gu Xun Xu | 2021 | Protein & Cell2021，12，1： | 3 |
| 18 | Recent advances in developing small-molecule inhibitors against SARS-CoV-2显示文摘The COVID-19 pandemic caused by the novel SARS-CoV-2 virus has caused havoc across the entire world.Even though several COVID-19 vaccines are currently in distribution worldwide,with others in the pipeline,treatment modalities lag behind.Accordingly,researchers have been working hard to understand the nature of the virus,its mutant strains,and the pathogenesis of the disease in order to uncover possible drug targets and effective therapeutic agents.As the research continues,we now know the genome structure,epidemiological and clinical features,and pathogenic mechanism of SARS-CoV-2.Here,we summarized the potential therapeutic targets involved in the life cycle of the virus.On the basis of these targets,small-molecule prophylactic and therapeutic agents have been or are being developed for prevention and treatment of SARS-CoV-2 infection. | Rong Xiang Zhengsen Yu Yang Wang Lili Wang Shanshan Huo Yanbai Li Ruiying Liang Qinghong Hao Tianlei Ying Yaning Gao Fei Yu Shibo Jiang | 2022 | Acta Pharmaceutica Sinica B2022，12，4： | 3 |
| 19 | Quantitative deconvolution of autocorrelations and cross correlations from two-dimensional lifetime decay maps in fluorescence lifetime correlation spectroscopy显示文摘Fluorescence correlation spectroscopy(FCS) is a widely used method for measuring molecular diffusion and chemical kinetics. However, when a mixture of fluorescent species is taken into account, the conventional FCS method has limitations in extracting autocorrelations for different species and cross correlations between different species. Recently developed fluorescence lifetime correlation spectroscopy(FLCS) based on time-tagged time-resolved(TTTR) photon recording, which can record the global and micro arrival time for each individual photon, has been used to discriminate different species according to fluorescence lifetime. Here, based on two-dimensional lifetime decay maps constructed from TTTR photon stream, we have developed a quantitative lifetime-deconvolution FCS model(LDFCS) to extract precise chemical rates for chemical conversions in multi-species systems. The key point of LDFCS model is separation of different species according to the global distribution of fluorescence lifetime and then deconvolution of autocorrelations and cross-correlations from the two-dimensional lifetime decay maps constructed by the micro arrival times of photon pairs at each delay time. | Shanshan Gao Menghua Cui Ruiru Li Ling Liang Ying Liu Liming Xie | 2017 | Science Bulletin2017，62，1： | 2 |
| 20 | Effect of formulation variables on in vitro release of a water-soluble drug from chitosanesodium alginate matrix tablets显示文摘The objective of this study is to investigate the feasibility of using chitosanesodium alginate(CSeSA)based matrix tablets for extended-release of highly water-soluble drugs by changing formulation variables.Using trimetazidine hydrochloride(TH)as a water-soluble model drug,influence of dissolution medium,the amount of CSeSA,the CS:SA ratio,the type of SA,the type and amount of diluents,on in vitro drug release from CSeSA based matrix tablets were studied.Drug release kinetics and release mechanisms were elucidated.In vitro release experiments were conducted in simulated gastric fluid(SGF)followed by simulated intestinal fluid(SIF).Drug release rate decreased with the increase of CSeSA amount.CS:SA ratio had only slight effect on drug release and no influence of SA type on drug release was found.On the other hand,a large amount of water-soluble diluents could modify drug release profiles.It was found that drug release kinetics showed the best fit to Higuchi equation with Fickian diffusion as the main release mechanism.In conclusion,this study demonstrated that it is possible to design extended-release tablets of watersoluble drugs using CSeSA as the matrix by optimizing formulation components,and provide better understanding about drug release from CSeSA matrix tablets. | Liang Li Jinfeng Li Shanshan Si Linlin Wang Chenjun Shi Yujiao Sun Zhenglin Liang Shirui Mao | 2015 | Asian Journal of Pharmaceutical Sciences2015，10，4： | 2 |
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