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| 1 | From Golden Rice to aSTARice:Bioengineering Astaxanthin Biosynthesis in Rice Endosperm显示文摘Carotenoids are important phytonutrients with antioxidant properties,and are widely used in foods and feedstuffs as Supplements.Astaxanthin,a red-colored ketocarotenoid,has strong antioxidant activity and thus can benefit human health.However,astaxanthin is not produced in most higher plants.Here we report the bioengineering of astaxanthin biosynthesis in rice endosperm by introducing four synthetic genes,sZmPSY1,sPaCrtl,sCrBKT,and sHpBHY,which encode the enzymes phytoene synthase,phytoene desaturase,β-carotene ketolase,and β-carotene hydroxylase,respectively.Transgneic overexpression of two (sZmPSY1 and sPaCrtl),three (sZmPSY1,sPaCrtl and sCrBKT),and all these four genes driven by rice endosperm-specific promoters established the Carotenoid/ketocarotenoid/astaxanthin biosynthetic pathways in the endosperm and thus resulted in various types of germplasm,from the yellow-grained β-caro- tene-enriched Golden Rice to orange-red-grained Canthaxanthin Rice and Astaxanthin Rice,respectively. Grains Of Astaxanthin Rice were enriched with astaxanthin in the endosperm and had higher antioxidant activity.These results proved that introduction of a minimal set of four transgenes enables de novo biosynthesis of astaxanthin in therice endosperm.This work provides a Successful example for synthetic biology in plants and biofortification in crops;the biofortified rice products generated by this study could be consumed as health-promoting foods and processed tO produce dietary supplements. | Qinlong Zhu Dongchang Zeng Suize Yu Chaojun Cui Jiamin Li Heying Li Junyu Chen Runzhao Zhang Xiucai Zhao Letian Chen Yao-Guang Liu | 2018 | Molecular Plant2018，11，12： | 35 |
| 2 | A small natural molecule promotes mitochondrial fusion through inhibition of the deubiquitinase USP30显示文摘Mitochondrial 熔化是为正常 mitochondrial 功能和 mitochondrial DNA 继承混合并且统一 mitochondrial 分隔空间的一个高度协调的过程。Dysregulated mitochondrial 熔化原因 mitochondrial 破碎,反常 mitochondrial 生理学和继承,并且有原因地与很多 neuronal 疾病被连接了。这里,我们识别了 potently 导致了 mitochondrial 熔化在在 Mfn1 或 Mfn2 是缺乏的房间恢复 mitochondrial 网络和氧化呼吸的 diterpenoid 衍生物 15-oxospiramilactone (S3 ) 。线粒体局部性的 deubiquitinase USP30 是 S3 的一个目标。由 S3 的 USP30 的抑制导致 Mfn1/2 的 non-degradative ubiquitination 的增加,它提高 Mfn1 和 Mfn2 活动并且支持 mitochondrial 熔化。通过 USP30 的一个禁止者的使用,因此,我们的学习揭开在支持 mitochondrial 的 Mfns 的 non-degradative ubiquitination 的异乎寻常的功能熔化。 | Wen Yue Ziheng Chen HaiYang Li Chen Yan Ming Chen Du Feng Chaojun Yan Hao Wu Lei Du Yueying Wang Jinhua Liu Xiaohu Huang Laixin Xia Lei Liu Xiaohui Wang Haijing Jin Jun Wang Zhiyin Song Xiaojiang Hao Quan Chen | 2014 | Cell Research2014，24，4： | 18 |
| 3 | T-cell receptor-engineered T cells for cancer treatment: current status and future directions显示文摘T 房间受体(TCR ) 设计了 T 房间为采纳房间治疗的一种新奇选择为癌症的几种先进形式的治疗被使用。用设计 TCR 的 T 房间的工作超过二十年以前开始了,与证明如此的房间能调停的众多的现出症状之前的潜的研究肿瘤细胞溶解和根除。这些试用的成功为临床的试用提供了基础,包括用到目标纽约食道的有鳞的房间癌(NY-ESO-1 ) 的设计 TCR 的 T 房间的最近的临床的成功。这些成功表明这条途径的潜力治疗癌症。在这评论,我们为癌症的治疗在设计 TCR 的 T 房间的现在和将来的应用上提供一个观点。我们的摘要集中于 TCR 激活和设计 TCR 的 T 房间的现出症状之前的潜、临床的应用。我们也讨论怎么提高设计 TCR 的 T 房间的功能并且在肿瘤微型环境延长他们的长寿。 | Yu Ping Chaojun Liu Yi Zhang | 2018 | Protein & Cell2018，9，3： | 16 |
| 4 | Overexpression of cotton (Gossypium hirsutum) dirigentl gene enhances lignification that blocks the spread of Verticillium dahliae显示文摘Dirigent 总科在整个植物王国丰富,特别脉管的植物。阐明棉花(Gossypium hirsutum ) 的功能在木质化的 DIR 基因,编码通常认为的 dirigent 蛋白质的二 cDNAs (指明的 GhDIR1 和 GhDIR2 ) 从棉花 cDNA 图书馆被孤立。实时量的反向的抄写聚合酶链反应分析表明那个 GhDIR1 抄本优先地在棉花胚轴被积累,而 GhDIR2 主要在棉花纤维被表示。GhDIR1 基因的 Overexpression 导致了转基因的棉花植物的木质素内容的增加,与野类型的相比。组织化学的试金表明转基因的植物在外皮和脉管的捆比野类型的显示了更普遍的木质化。而且,转基因的棉花植物显示了更多的忍耐到 Verticillium dahliae 的感染。我们的数据建议 GhDIR1 可以涉及能堵住真菌的病原体 V 的传播的棉花木质化。dahliae。 | Haiyan Shi Zhihao Liu Li Zhu Chaojun Zhang Yun Chen Ying Zhou Fuguang Li Xuebao Li | 2012 | Acta Biochimica et Biophysica Sinica2012，44，7： | 12 |
| 5 | Synthesis of graphene materials by electrochemical exfoliation: Recent progress and future potential显示文摘Synthesis of structurally controlled graphene materials is critical for realizing their practical applications.The electrochemical exfoliation of graphite has emerged as a simple method to produce graphene materials.This review examines research progress in the last 5 years,from 2015 to 2019.Graphene material synthesis methods generally have a trade‐off between increasing production yield and achieving better material property control.The synthesis conditions for synthesizing pristine graphene,graphene oxide(GO),and graphene composites are significantly different.Thus,in this review,we first discuss synthesis methods for graphene materials with high C/O ratios from four aspects:graphite electrodes,equipment engineering,electrolytes,and additional reduction methods.Next,we survey synthesis methods for GO and examine how the pretreatment of the graphite electrodes,electrolytes,and operation parameters,such as applied voltages,electrolyte temperatures,and mechanical forces,affect the quality of GO.Further,we summarize electrochemical exfoliation methods used to dope graphene materials,introduce covalent functional groups,incorporate various nanoparticles,and assembly of graphene architectures.For all synthesis methods,we compare the properties of resulting graphene materials such as C/O ratios,lateral size,layer numbers,and quality characterized by Raman spectroscopy.Lastly,we propose our perspectives on further research.We hope this review stimulates more studies to realize the on‐demand production of graphene materials with desired properties using electrochemical exfoliation methods. | Fei Liu Chaojun Wang Xiao Sui Muhammad Adil Riaz Meiying Xu Li Wei Yuan Chen | 2019 | Carbon Energy2019，1，2： | 6 |
| 6 | Transcriptome Profiling Reveals Auxin and Cytokinin Regulating Somatic Embryogenesis in Different Sister Lines of Cotton Cultivar CCRI24显示文摘在在棉花(Gossypium hirsutum L.) 位于体的 embryogenesis (SE ) 下面的分子的机制上得到一个更宽广的看法,棉花 transcriptome 动力学的全球分析用 RNA-Seq 在在不同姐妹线的 SE 期间被执行。204349 unigenes 的一个总数被 214977462 Illumina 的汇编读的 de novo 检测。量的反向的 transcriptionpolymerase 链反应(qRT-PCR ) 大小断然与几乎所有测试基因的 RNA-Seq 结果被相关(R2=0.841,关联在 0.01 水平是重要的) 。不同植物激素(植物生长素和细胞激动素) 在媒介和在在不同姐妹线的二个阶段的这二植物激素的内长的内容变化的集中比率在棉花 SE 期间建议了植物生长素和细胞激动素的角色。根据植物激素相关的基因的全球基因规定,从所有差别的众多的基因表示了抄本涉及植物生长素和细胞激动素生合成和信号 transduction 小径。差别的分析表示了涉及揭示的这些小径的基因在在二根姐妹线之间的基因类型和丰富的实质的变化。隔离,克隆并且在棉花 SE 期间揭示了显著起来表示或下面表示的基因是的 silencing/overexpressing 重要。而且,植物生长素和细胞激动素与对方或另外的因素遗体一起在 SE,而是潜在的串音起一个主要作用不清楚。 | Zhenzhen Xu Chaojun Zhang Xueyan Zhang Chuanliang Liu Zhixia Wu Zuoren Yang Kehai Zhou Xiaojie Yang Fuguang Li | 2013 | Journal of Integrative Plant Biology2013，55，7： | 5 |
| 7 | Coupled Model of Two-phase Debris Flow,Sediment Transport and Morphological Evolution显示文摘The volume fraction of the solid and liquid phase of debris flows,which evolves simultaneously across terrains,largely determines the dynamic property of debris flows. The entrainment process significantly influences the amplitude of the volume fraction. In this paper,we present a depth-averaged two-phase debris-flow model describing the simultaneous evolution of the phase velocity and depth,the solid and fluid volume fractions and the bed morphological evolution. The model employs the Mohr–Coulomb plasticity for the solid stress,and the fluid stress is modeled as a Newtonian viscous stress. The interfacial momentum transfer includes viscous drag and buoyancy. A new extended entrainment rate formula that satisfies the boundary momentum jump condition(Iverson and Ouyang,2015) is presented. In this formula,the basal traction stress is a function of the solid volume fraction and can take advantage of both the Coulomb and velocity-dependent friction models. A finite volume method using Roe's Riemann approximation is suggested to solve the equations. Three computational cases are conducted and compared with experiments or previous results. The results show that the current computational model and framework are robust and suitable for capturing the characteristics of debris flows. | HE Siming OUYANG Chaojun LIU Wei WANG Dongpo | 2016 | Acta Geologica Sinica(English Edition)2016，90，6： | 5 |
| 8 | Analysis of genetic alterations identifies the frequent mutation of GNAS in colorectal laterally spreading tumors显示文摘Dear Editor,Colorectal cancer(CRC)is among the most commonly diagnosed cancer and the leading cause of cancer-related death worldwide[1].Most CRCs develop from adenomas.Laterally spreading tumors(LSTs)are non-polypoid superficial colorectal neoplasms(CRNs)that are larger than 1 cm and typically extend laterally along the luminal wall.Based on surface morphology,it can be classified into a granular or nongranular type.The pathological morphology of LST manifests only as adenoma or with cancer.Recent studies have characterized 45-79%of LSTs as high-grade intraepithelial neoplasia(HGIN)or submucosal invasion;and the risk of submucosal invasion increases with lesion size[2].Hence,LST is considered a precancerous CRC lesion.Patients with LSTs are usually asymptomatic and diagnosis typically occurs during physical examinations or screening colonoscopy. | Lei Pan Junsheng Chen Chaojun Zhu Lu Han Aimin Li Side Liu | 2020 | Cancer Communications2020，40，11： | 3 |
| 9 | Scientific challenges of research on natural hazards and disaster risk显示文摘As a discipline,the science of natural hazards and disaster risk aims to explain the spatial-temporal pattern,process and mechanism,emergency response and risk mitigation of natural hazards,which requires a multidisci-plinary and interdisciplinary approach.With the support of Natural Science Finance of China(NSFC)and Chinese Academy of Sciences(CAS),in-depth research and systematic analysis on natural hazards and disaster risk were conducted.In this paper,the state of the art in research on natural hazards is summarized from seven aspects:formation process,mechanism and dynamic of natural hazards,disaster risk assessment,forecast,monitoring and early warning,disaster mitigation,emergency treatment and rescue,risk management and post-disaster re-construction.The trends within the natural hazards and disaster risk as a discipline were identified,along with existing shortcomings and significant gaps that need to be addressed.This paper highlighted:1)the scientific challenges including the frontier scientific issues and technological gaps on natural hazards and disaster risk dis-cipline from 2025 to 2035 in China,and 2)the proposal to develop a systemic and holistic natural hazards and disaster risk discipline. | Peng Cui Jianbing Peng Peijun Shi Huiming Tang Chaojun Ouyang Qiang Zou Lianyou Liu Changdong Li Yu Lei | 2021 | Geography and Sustainability2021，2，3： | 3 |
| 10 | Two-dimensional Dynamics Simulation of Two-phase Debris Flow显示文摘To investigate the movement mechanism of debris flow, a two-dimensional, two-phase, depthintegrated model is introduced. The model uses Mohr-Coulomb plasticity for the solid rheology, and the fluid stress is modeled as a Newtonian fluid. The interaction between solid and liquid phases, which plays a major role in debris flow movement, is assumed to consist of drag and buoyancy forces. The applicability of drag force formulas is discussed. Considering the complex interaction between debris flow and the bed surface, a combined friction boundary condition is imposed on the bottom, and this is also discussed. To solve the complex model equations, a numerical method with second-order accuracy based on the finite volume method is proposed. Several numerical experiments are performed to verify the feasibilities of model and numerical schemes. Numerical results demonstrate that different solid volume fractions substantially affect debris flow movement. | LIU Wei HE Siming OUYANG Chaojun | 2017 | Acta Geologica Sinica(English Edition)2017，91，5： | 2 |
| 11 | An enhanced method for nucleic acid detection with CRISPR-Cas12a using phosphorothioate modified primers and optimized gold-nanopaticle strip显示文摘CRISPR-Cas12a system has been shown promising for nucleic acid diagnostics due to its rapid,portable and accurate features.However,cleavage of the amplicons and primers by the cis-and trans-activity of Cas12a hinders the attempts to integrate the amplification and detection into a single reaction.Through phosphorothioate modification of primers,we realized onepot detection with high sensitivity using plasmids of SARS-CoV-2,HPV16 and HPV18.We also identified the activated Cas12a has a much higher affinity to C nucleotide-rich reporter than others.By applying such reporters,the reaction time required for a lateral-flow readout was significantly reduced.Furthermore,to improve the specificity of the strip-based assay,we created a novel reporter and,when combined with a customized gold-nanopaticle strip,the readout was greatly enhanced owing to the elimination of the nonspecific signal.This established system,termed Targeting DNA by Cas12a-based Eye Sight Testing in an Onepot Reaction(TESTOR),was validated using clinical cervical scrape samples for human papillomaviruses(HPVs)detection.Our system represents a general approach to integrating the nucleic acid amplification and detection into a single reaction in CRISPR-Cas systems,highlighting its potential as a rapid,portable and accurate detection platform of nucleic acids. | Jiaojiao Gong Lijuan Kan Xiuming Zhang Ying He Jiaqiang Pan Liping Zhao Qianyun Li Menghao Liu Jie Tian Sili Lin Zhouyu Lu Liang Xue Chaojun Wang Guanghui Tang | 2021 | Bioactive Materials2021，6，12： | 1 |
| 12 | A Modified Genetic Algorithm Based Optimisation of Milling Parameters 显示文摘 | Yanming Liu Chaojun Wang | 1999 | International Journal of Advanced Manufacturing Technology1999，，15： | 1 |
| 13 | Chalcocite(bio)hydrometallurgy-current state,mechanism,and future directions:A review显示文摘There has been a strong interest in technologies suited for mining and processing of low-grade ores because of the rapid depletion of mineral resources in the world.In most cases,the extraction of copper from such raw materials is achieved by applying the leaching procedures.However,its low extraction efficiency and the long extraction period limit its large-scale commercial applications in copper recovery,even though bioleaching has been widely employed commercially for heap and dump bioleaching of secondary copper sulfide ores.Overcoming the technical challenges requires a better understanding of leaching kinetics and on-site microbial activities.Herein,this paper reviews the current status of main commercial biomining operations around the world,identifies factors that affect chalcocite dissolution both in chemical leaching and bioleaching,summarizes the related kinetic research,and concludes with a discussion of two on-site chalcocite heap leaching practices.Further,the challenges and innovations for the future development of chalcocite hydrometallurgy are presented in the end. | Shichao Yu Rui Liao Baojun Yang Chaojun Fang Zhentang Wang Yuling Liu Baiqiang Wu Jun Wang Guanzhou Qiu | 2022 | Chinese Journal of Chemical Engineering2022，35，1： | 1 |
| 14 | Large Margin hidden Markov Models for Speech Recognition 显示文摘 | Hui Jiang Xinwei Li Chaojun Liu | 2006 | IEEE Transactions on Audio Speech and Language Processing2006，9，5： | 1 |
| 15 | Human activity and climate change triggered the expansion of rocky desertification in the karst areas of Southwestern China显示文摘It is conducive to the sustainable development of human beings in karst regions to research the mechanism of karst rocky desertification(KRD)expansion.Whether the large-scale KRD in southwestern China is caused by climate change or human activities is still controversial.In this study,the evolution of the KRD in southwestern China over the past 2000 years was reconstructed through the high-precisionδ^(13)C record of stalagmites from Shijiangjun(SJJ)Cave,Guizhou Province,China.Theδ^(13)C of the stalagmites from SJJ Cave exhibited heavy values from the Medieval Warm Period(MWP)to the Little Ice Age(LIA).Furthermore,theδ^(13)C records of other stalagmites and tufa from southwestern China also showed the same significant heavy trend.Because the stalagmiteδ^(13)C could record the change of ecological environment,it indicated that the consistent change of the stalagmitesδ^(13)C may record the process of KRD expansion in the karst regions of southwestern China.During the MWP,the stronger Asian summer monsoon and the northward movement of the rain belt led to a dry period in southwestern China and a wet period in northern China.In contrast,it was wet in southwestern China and dry in northern China during the LIA.In addition,after the Jing-Kang event(JK event,AD1127)occurred at the end of the Northern Song dynasty,the political and economic center of China migrated to southern China for the first time,which changed the population distribution pattern of larger population in the north and smaller population in the south.Therefore,the expansion of KRD in southwestern China was exacerbated in the MWP due to the change of climate in southwestern China,the migration of a large number of people,wars,the large-scale reclamation of arable land,and the cultivation of large areas of crops. | Chaojun CHEN Daoxian YUAN Hai CHENG Tsailuen YU Chuanchou SHEN RLawrence EDWARDS Yao WU Siya XIAO Jian ZHANG Tao WANG Ran HUANG Ziqi LIU Tingyong LI Junyun LI | 2021 | Science China Earth Sciences2021，64，10： | 1 |
| 16 | New Subclasses of Biholomorphic Mappings and the Modified Roper-Suffridge Operator显示文摘The authors propose a new approach to construct subclasses of biholomorphic mappings with special geometric properties in several complex variables. The RoperSuffridge operator on the unit ball B^n in C^n is modified. By the analytical characteristics and the growth theorems of subclasses of spirallike mappings, it is proved that the modified Roper-Suffridge operator [Φ_(G,γ)(f)](z) preserves the properties of S_Ω~*(A, B), as well as strong and almost spirallikeness of type β and order α on B^n. Thus, the mappings in S_Ω~*(A, B), as well as strong and almost spirallike mappings, can be constructed through the corresponding functions in one complex variable. The conclusions follow some special cases and contain the elementary results. | Chaojun WANG Yanyan CUI Hao LIU | 2016 | Chinese Annals of Mathematics,Series B2016，37，5： | 1 |
| 17 | Co_(1-x)S embedded in porous carbon derived from metal organic framework as a highly efficient electrocatalyst for oxygen evolution reaction显示文摘Developing active, robust, and cost-efficient electrocatalysts is critical for oxygen evolution reaction(OER). Here, a novel composite catalyst of Co_(1-x)S embedded in porous dodecahedron carbon hybrid was synthesized by a two-step conversion protocol of a cobalt-based metal-organic framework(ZIF-67). The porous dodecahedron Co_(1-x)S@C composite catalyst was prepared by direct oxidation of ZIF-67 followed by sulfurization reaction. The Co_(1-x)S@C composite exhibit superior OER performance, including a low overpotential of 260 mV for 10 mA/cm2, a small Tafel slope of ~85 mV/dec, outstanding stability over 80 h and almost 100% Faradaic efficiency. The various material characterizations indicate that the excellent activity and strong stability of Co_(1-x)S@C might be attributed to good conductivity of Co_(1-x)S, mesoporous nanostructure, and synergistic effect of Co_(1-x)S encapsulated within porous carbon. This work provides a novel strategy for designing and synthesizing advanced composite | Denghong He Xiaolin Wu Wei Liu Chaojun Lei Chunlin Yu Guokui Zheng Junjie Pan Lecheng Lei Xingwang Zhang | 2019 | Chinese Chemical Letters2019，30，1： | 1 |
| 18 | CuS/RGO hybrid by one-pot hydrothermal method for efficient electrochemical sensing of hydrogen peroxide显示文摘In this study,a non-enzymatic hydrogen peroxide sensor was successfully fabricated on the basis of copper sulfide nanoparticles/reduced graphene oxide(CuS/RGO) electrocatalyst.Using thiourea as reducing agent and sulfur donor,CuS/RGO hybrid was synthesized through a facile one-pot hydrothermal method,where the reduction of GO and deposition of CuS nanoparticles on RGO occur simultaneously.The results confirmed that the CuS/RGO hybrid helps to prevent the aggregation of CuS nanoparticles.Electrochemical investigation showed that the as-prepared hydrogen peroxide sensor exhibited a low detection limit of 0.18μmol/L(S/N = 3),a good reproducibility(relative standard deviation(RSD) of4.21%),a wide linear range(from 3 to 1215 μmol/L) with a sensitivity of 216.9 μA L/mmol/cm^2 under the optimal conditions.Moreover,the as-prepared sensor also showed excellent selectivity and stability for hydrogen peroxide detection.The excellent performance of CuS/RGO hybrid,especially the lower detection limit than certain enzymes and noble metal nanomaterials ever reported,makes it a promising candidate for non-enzymatic H_2O_2 sensors. | Wei Liu Chaojun Lei Hongxiu Zhang Xiaolin Wu Qing Jia Denghong He Bin Yang Zhongjian Li Yang Hou Lecheng Lei Xingwang Zhang | 2017 | Chinese Chemical Letters2017，28，6： | 1 |
| 19 | Novel systemic lupus erythematosus autoantigens identified by human protein microarray technology显示文摘 | Wei Huang Chaojun Hu Haipan Zeng Ping Li Lei Guo Xiaofeng Zeng Guozhen Liu Fengchun Zhang Yongzhe Li Lin Wu | 2012 | Biochemical and Biophysical Research Communications2012，，2： | 1 |
| 20 | A Serum Biomarker Model to Diagnose Pancreatic Cancer Using Proteomic Fingerprint Technology显示文摘OBJECTIVE To establish a serum protein pattern model for screening pancreatic cancer. METHODS Twenty-nine serum samples from patients with pancreatic cancer were collected before surgery,and an additional 57 serum samples from age and sex-matched individuals without cancer were used as controls.WCX magnetic beans and a PBS Ⅱ-C protein chip reader (Ciphergen Biosystems Inc) were employed to detect the protein fingerprint expression of all serum samples. The resulting profiles comparing serum from cancer and normal patients were analyzed with the Biomarker Wizard system,to establish a model using the Biomarker Pattern system software. A double-blind test was used to determine the sensitivity and specificity of the model. RESULTS A group of 4 biomarkers(relative molecular weights were 5,705 Da,4,935 Da,5,318 Da,3,243 Da) were selected to set up a decision tree to produce the classification model to effectively screen pancreatic cancer patients.The results yielded a sensitivity of 100%(20/20),specificity of 97.4% (37/38).The ROC curve was 99.7%.A double-blind test used to challenge the model resulted in a sensitivity of 88.9% and a specificity of 89.5%. CONCLUSION New serum biomarkers of pancreatic cancer have been identified.The pa ern of combined markers provides a powerful and reliable diagnostic method for pancreatic cancer with high sensitivity and specificity. | Chunlin Ge Ning Ma Dianbo Yao Fengming Luan Chaojun Hu Yongzhe Li Yongfeng Liu | 2008 | Chinese Journal of Clinical Oncology2008，5，3： | 0 |
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