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| 1 | From Golden Rice to aSTARice:Bioengineering Astaxanthin Biosynthesis in Rice Endosperm显示文摘Carotenoids are important phytonutrients with antioxidant properties,and are widely used in foods and feedstuffs as Supplements.Astaxanthin,a red-colored ketocarotenoid,has strong antioxidant activity and thus can benefit human health.However,astaxanthin is not produced in most higher plants.Here we report the bioengineering of astaxanthin biosynthesis in rice endosperm by introducing four synthetic genes,sZmPSY1,sPaCrtl,sCrBKT,and sHpBHY,which encode the enzymes phytoene synthase,phytoene desaturase,β-carotene ketolase,and β-carotene hydroxylase,respectively.Transgneic overexpression of two (sZmPSY1 and sPaCrtl),three (sZmPSY1,sPaCrtl and sCrBKT),and all these four genes driven by rice endosperm-specific promoters established the Carotenoid/ketocarotenoid/astaxanthin biosynthetic pathways in the endosperm and thus resulted in various types of germplasm,from the yellow-grained β-caro- tene-enriched Golden Rice to orange-red-grained Canthaxanthin Rice and Astaxanthin Rice,respectively. Grains Of Astaxanthin Rice were enriched with astaxanthin in the endosperm and had higher antioxidant activity.These results proved that introduction of a minimal set of four transgenes enables de novo biosynthesis of astaxanthin in therice endosperm.This work provides a Successful example for synthetic biology in plants and biofortification in crops;the biofortified rice products generated by this study could be consumed as health-promoting foods and processed tO produce dietary supplements. | Qinlong Zhu Dongchang Zeng Suize Yu Chaojun Cui Jiamin Li Heying Li Junyu Chen Runzhao Zhang Xiucai Zhao Letian Chen Yao-Guang Liu | 2018 | Molecular Plant2018，11，12： | 35 |
| 2 | Vulnerability of Water Resources and Its Spatial Heterogeneity in Haihe River Basin, China显示文摘To manage water resources effectively, a multiscale assessment of the vulnerability of water resources on the basis of political boundaries and watersheds is necessary. This study addressed issues on the vulnerability of water resources and provided a multiscale comparison of spatial heterogeneity under a climate change background. Using improved quantitative evaluation methods of vulnerability, the Theil index and the Shannon-Weaver index, we evaluated the vulnerability of water resources and its spatial heterogeneity in the Haihe River Basin in four scales, namely, second-class water resource regions(Class ⅡWRRs), third-class water resource regions(Class Ⅲ WRRs), Province-Class ⅡWRRs, and Province-Class Ⅲ WRRs. Results show that vulnerability enhances from the north to south in the different scales, and shows obvious spatial heterogeneity instead of moving toward convergence in multiscale assessment results. Among the Class Ⅱ WRRs, the Tuhai-Majia River is the most vulnerable area, and the vulnerability of the Luanhe River is lower than that of the north of the Haihe River Basin, which in turn is lower than that of the south of the Haihe River Basin. In the scales of Class Ⅲ WRRs and Province-Class Ⅲ WRRs, the vulnerability shows obvious spatial heterogeneity and diversity measured by the Theil index and the Shannon-Weaver index. Multiscale vulnerability assessment results based on political boundaries and the watersheds of the Haihe River Basin innovatively provided in this paper are important and useful to characterize the real spatial pattern of the vulnerability of water resources and improve water resource management. | XIA Jun CHEN Junxu WENG Jianwu YU Lei QI Junyu LIAO Qiang | 2014 | Chinese Geographical Science2014，24，5： | 9 |
| 3 | An effective strategy to establish a male sterility mutant mini-library by CRISPR/Cas9-mediated knockout of anther-specific genes in rice显示文摘Male sterility(MS),characterized by functional defects in male organs or gametes,is an important agronomic trait for hybrid seed production,especially for self-pollinated crops such as rice(Oryza sativa L)(Chen and Liu,2014).Spontaneous MS mutants are rare and difficult to maintain in nature,thus limiting basicresearch and breeding.Artificial mutants are typically generated by physical,chemical,or biological mutagenesis(Wei et al,2013).Recently developed genome editing systems such as CRISPR/Cas9 allow efficient and timesaving knockout of endogenous genes at specific sites(Smith et al,2000;Moscou and Bogdanove,2009;Gasiunas et al.,2012). | Kun Ma Jingluan Han Yu Hao Zhongfang Yang Junyu Chen Yao-Guang Liu Qinlong Zhu Letian Chen | 2019 | Journal of Genetics and Genomics2019，46，5： | 6 |
| 4 | Dissection of two quantitative trait loci for grain weight linked in repulsion on the long arm of chromosome 1 of rice(Oryza sativa L.)显示文摘Grain weight is a key determinant of grain yield in rice. Three sets of rice populations with overlapping segregating regions in isogenic backgrounds were established in the generations of BC2 F5, BC2 F6 and BC2 F7, derived from Zhenshan 97 and Milyang 46, and used for dissection of quantitative trait loci(QTL) for grain weight. Two QTL linked in repulsion phase on the long arm of chromosome 1 were separated. One was located between simple sequence repeat(SSR) markers RM11437 and RM11615, having a smaller additive effect with the enhancing allele from the maintainer line Zhenshan 97 and a partially dominant effect for increasing grain weight. The other was located between SSR markers RM11615 and RM11800, having a larger additive effect with the enhancing allele from the restorer line Milyang 46 and a partially dominant effect for increasing grain weight. When the two QTL segregated simultaneously, a residual additive effect with the enhancing allele from Milyang 46 and an over-dominance effect for increasing grain weight were detected. This suggests that dominant QTL linked in repulsion phase might play an important role in heterosis in rice. Our study also indicates that the use of populations with overlapping segregating regions in isogenic backgrounds is helpful for the dissection of minor linked QTL. | Liang Guo Kai Wang Junyu Chen Derun Huang Yeyang Fan Jieyun Zhuang | 2013 | The Crop Journal2013，1，1： | 6 |
| 5 | A novel selective autophagy receptor,CCDC50,delivers K63 polyubiquitination-activated RIG-I/MDA5 for degradation during viral infection显示文摘Autophagy is a con served process that delivers cytosolic substa nces to the lysosome for degradatio n,but its direct role in the regulation of antiviral inn ate immu nity remains poorly un derstood.Here,through high-throughput screeni ng,we discovered that CCDC50 functions as a previously unknown autophagy receptor that negatively regulates the type I interferon(IFN)signaling pathway initiated by RIG-I-like receptors(RLRs),the sensors for RNA viruses.The expression of CCDC50 is enhanced by viral infection,and CCDC50 specifically recognizes K63-polyubiquitinated RLRs,thus delivering the activated RIG-I/MDA5 for autophagic degradation.The associatio n of CCDC50 with phagophore membrane protei n LC3 is con firmed by crystal structure analysis.In contrast to other known autophagic cargo receptors that associate with either the LIR-docking site(LDS)or the UIMdocking site(UDS)of LC3,CCDC50 can bind to both LDS and UDS,representing a new type of cargo receptor.In mouse models with RNA virus infection,CCDC50 deficiency reduces the autophagic degradation of RIG-I/MDA5 and promotes type I IFN responses,resulting in enhaneed viral resistance and improved survival rates.These results reveal a new link between autophagy and antiviral innate immune resp on ses and provide additional in sights into the regulatory mecha nisms of RLR-mediated antiviral signaling. | Panpan Hou Kongxiang Yang Penghui Jia Lan Liu Yuxin Lin Zibo Li Jun Li Shuliang Chen Shuting Guo Ji'An Pan Junyu Wu Hong Peng Weijie Zeng Chunmei Li Yingfang Liu Deyin Guo | 2021 | Cell Research2021，31，1： | 5 |
| 6 | Fermented mixed feed alters growth performance,carcass traits,meat quality and muscle fatty acid and amino acid profiles in finishing pigs显示文摘This study was conducted to investigate the effects of fermented mixed feed(FMF)on growth performance,carcass traits,meat quality,muscle amino acid and fatty acid composition and mRNA expression levels of genes related to lipid metabolism in finishing pigs.In the present study,144 finishing pigs(Duroc×Berkshire×Jiaxing Black)were randomly allocated to 3 dietary treatments with 4 replicate pens per group and 12 pigs per pen.The dietary treatments included a basal diet(CON),a basal diet+5%FMF and a basal diet+10%FMF.The experiment lasted 38 d after 4 d of acclimation.The results showed that 5%and 10%FMF significantly increased the average daily gain(ADG)of the females but not the males(P<0.05),but FMF supplementation showed no impact on carcass traits.Moreover,10%FMF supplementation increased the meat color45 min and meat color24 h values,while it decreased the shear force relative to CON(P<0.05).In addition,10%FMF significantly increased the contents of flavor amino acids(FAA),total essential AA(EAA),total non-EAA(NEAA)and total AA relative to CON(P<0.05).Furthermore,the diet supplemented with 10%FMF significantly increased the concentration of n-3 polyunsaturated fatty acids(PUFA),n-6 PUFA and total PUFA,and the PUFA to saturated fatty acids ratio(P<0.05),suggesting that FMF supplementation increased meat quality.Moreover,compared with the CON,10%FMF supplementation increased the mRNA expression of lipogenic genes,including CEBPα,PPARγ,SREBP1 and FABP4,and upregulated the expression of unsaturated fatty acid synthesis(ACAA1 and FADS2).Together,our results suggest that 10%FMF dietary supplementation improved the female pigs’growth performance,improved the meat quality and altered the profiles of muscle fatty acids and amino acids in finishing pigs.This study provides a reference for the production of high-quality pork. | Shiqi Liu Man Du Yuang Tu Wenjing You Wentao Chen Guoliang Liu Junyue Li Yizhen Wang Zeqing Lu Tenghao Wang Tizhong Shan | 2023 | Animal Nutrition2023，，1： | 4 |
| 7 | Lotus seedpod-inspired internal vascularized 3D printed scaffold for bone tissue repair显示文摘In the field of bone defect repair,3D printed scaffolds have the characteristics of personalized customization and accurate internal structure.However,how to construct a well-structured vascular network quickly and effectively inside the scaffold is essential for bone repair after transplantation.Herein,inspired by the unique biological structure of“lotus seedpod”,hydrogel microspheres encapsulating deferoxamine(DFO)liposomes were prepared through microfluidic technology as“lotus seeds”,and skillfully combined with a three-dimensional(3D)printed bioceramic scaffold with biomimetic“lotus”biological structure which can internally grow blood vessels.In this composite scaffold system,DFO was effectively released by 36%in the first 6 h,which was conducive to promote the growth of blood vessels inside the scaffold quickly.In the following 7 days,the release rate of DFO reached 69%,which was fundamental in the formation of blood vessels inside the scaffold as well as osteogenic differentiation of bone mesenchymal stem cells(BMSCs).It was confirmed that the composite scaffold could significantly promote the human umbilical vein endothelial cells(HUVECs)to form the vascular morphology within 6 h in vitro.In vivo,the composite scaffold increased the expression of vascularization and osteogenic related proteins Hif1-α,CD31,OPN,and OCN in the rat femoral defect model,significantly cutting down the time of bone repair.To sum up,this“lotus seedpod”inspired porous bioceramic 3D printed scaffold with internal vascularization functionality has broad application prospects in the future. | Xiaoyu Han Mingjie Sun Bo Chen Qimanguli Saiding Junyue Zhang Hongliang Song Lianfu Deng Peng Wang Weiming Gong Wenguo Cui | 2021 | Bioactive Materials2021，6，6： | 4 |
| 8 | Nanoelectrochemical architectures for high-spatial-resolution single cell analysis显示文摘The analysis of single cells instead of cell populations is important for characterizing cellular heterogeneity and elucidating the cellular signalling pathways. Nanoelectrodes have emerged as an increasingly important tool for biomolecule analyses at the single-cell level with high spatial or temporal resolution. Various electrochemical methods, such as amperometry and scanning electrochemical microscopy(SECM), have been applied. Research to date has focused on the development of new nanoelectrochemical architectures, such as arrays, to achieve higher spatial resolution and faster analysis rates for single-cell analysis. In this review, the fabrication of these new nanoelectrochemical architectures and their applications in high spatial resolution single-cell analyses are discussed. The recent progress of Chinese researchers is highlighted. | Junyu Zhou Dechen Jiang Hong-Yuan Chen | 2017 | Science China Chemistry2017，60，10： | 3 |
| 9 | Identification of three novel noncoding RNAs from Drosophila melanogaster显示文摘三新奇小非编码的 RNA 从 D 的保存 intronicregions 被识别。由使用比较基因组学方法和分子的生物学途径的 melanogaster。Oneis 以一个 combinated 方法的新奇 snoRNA,它 snoRNAfamily 显示典型地代表 C/D 盒子的结构的特征并且为在 C2673 指导 D.melanogaster 28S rRNA 的 2''-O-methylation 拥有一个 10-nt-long rRNA 反感觉元素。另外的二是预言先锋采用已知的 miRNA 的 astem 环结构特征的 miRNAs。二 miRNAs 基因看起来有无所不在的表示侧面, ~ 23-nt RNA 抄本由北弄污检测了。我们的学习 revealed396 多种类 intronic 保存了与长度 from100 在 introns 嵌套到 500 bp 的序列(MCIS ) 。除了编码的小 RNA, MCIS 可能作为涉及基因抄写或 post-transcriptional 处理的 c/s-acting 元素工作。 | HE Hualiang ZHOU Hui XIAO Zhendong ZENG Xianfen CHEN Junyu ZHENG Tao QU Lianghu | 2006 | Chinese Science Bulletin2006，51，22： | 2 |
| 10 | Minor-effect QTL for heading date detected in crosses between indica rice cultivar Teqing and near isogenic lines of IR24显示文摘Identification of quantitative trait loci(QTL) having small effects on heading date(HD) is important for fine-tuning flowering time in rice(Oryza sativa L.). In this study, minor-effect QTL for HD were identified using five segregating rice populations, including a recombinant inbred line population derived from crosses between indica cultivar Teqing and near isogenic lines of IR24,and four populations derived from residual heterozygotes identified in the original population.HD data from these populations were obtained in multiple years or at two locations with different photoperiods. A total of 11 QTL were detected; they had small additive effects ranging from 0.21 to1.63 days. The QTL were all detected in different populations, locations and/or years, having consistent allelic effects across experiments and a stable magnitude across years at the same location. These QTL, and other minor-effect QTL that have been cloned or fine-mapped, generally do not have strong photoperiod sensitivity, and thus can be used in a wide range of ecogeographical conditions. Seven of the 11 QTL were different from those that have been cloned or fine-mapped, providing new candidates for gene cloning and marker-assisted breeding. Allelic effects of QTL corresponding to those that had been cloned or fine-mapped, were much smaller in this study than previously reported. The results supported the assumption that qualitative and quantitative genes may be different alleles at the same loci, suggesting that it may be promising to identify minor-effect QTL from major heading date genes/QTL that have been cloned. | Zhichao Sun Yujun Zhu Junyu Chen Hui Zhang Zhenhua Zhang Xiaojun Niu Yeyang Fan Jieyun Zhuang | 2018 | The Crop Journal2018，6，3： | 2 |
| 11 | Numerical model to quantify biogenic volatile organic compound emissions:The Pearl River Delta region as a case study显示文摘This article compiles the actual knowledge of the biogenic volatile organic compound(BVOC) emissions estimated using model methods in the Pearl River Delta(PRD) region, one of the most developed regions in China. The developed history of BVOC emission models is presented briefly and three typical emission models are introduced and compared. The results from local studies related to BVOC emissions have been summarized. Based on this analysis, it is recommended that local researchers conduct BVOC emission studies systematically, from the assessment of model inputs, to compiling regional emission inventories to quantifying the uncertainties and evaluating the model results. Beyond that,more basic researches should be conducted in the future to close the gaps in knowledge on BVOC emission mechanisms, to develop the emission models and to refine the inventory results. This paper can provide a perspective on these aspects in the broad field of research associated with BVOC emissions in the PRD region. | Xuemei Wang Shuping Situ Weihua Chen Junyu Zheng Alex Guenther Qi Fan Ming Chang | 2016 | Journal of Environmental Sciences2016，28，8： | 2 |
| 12 | Mechanical and Tribological Study of PVA–pMPDSAH Double-Network Hydrogel Prepared by Ultraviolet Irradiation and Freeze–Thaw Methods for Bionic Articular Cartilage显示文摘Hydrogel has been widely used in the research of bionic articular cartilage due to their similarity in structure and functional properties to natural articular cartilage.In this research,polyvinyl alcohol and betaine monomer were used as raw materials to prepare a high-strength double-network hydrogel by a combination of ultraviolet(UV)irradiation and freeze–thaw methods.The structure of samples was characterized by Fourier transform infrared spectroscopy and X-ray diff raction,and the morphology of the samples was characterized by scanning electron microscope and three-dimensional white light interferometer.In addition,we also studied the swelling ratio,water content,mechanical properties and tribological properties of the samples.We found that the addition of betaine monomer and the UV irradiation time had a positive eff ect on the mechanical properties and tribological properties of the samples. | Junyue Chen Lingling Cui Chengqi Yan Dangsheng Xiong | 2021 | Journal of Bionic Engineering2021，18，5： | 2 |
| 13 | PTEN suppresses tumorigenesis by directly dephosphorylating Akt显示文摘Dear Editor,The serine-threonine kinase Akt plays a central role in regulating cell proliferation,migration,angiogenesis,transformation,energy metabolism,and death.1 The stimulation of growth factors recruits the PI3K to the plasma membrane and phosphatidylinositol-3A5-trisphosphate(PIP3),which in turn recruits Akt to the plasma membrane through PH domain of Akt. | Lang Bu Huan Wang Ji-an Pan Lang Chen Fan Xing Junyu Wu Shun Li Deyin Guo | 2021 | Signal Transduction and Targeted Therapy2021，6，8： | 2 |
| 14 | Spatiotemporal Mapping of Salt Marshes in the Intertidal Zone of China during 1985-2019显示文摘This study mapped the areal extent,identified the species composition,and analyzed the changes of salt marshes in the intertidal zone of China during the period 1985–2019.With the aid of the cloud platform of the Google Earth Engine,we selected Landsat 5/8 and Sentinel-2 images and used the support vector machine classification method to extract salt marsh information for the years of 1985,1990,1995,2000,2005,2010,2015,and 2019.Seven major species of salt marshes:Phragmites australis,Suaeda spp.,Spartina alterniflora,Scirpus mariqueter,Tamarix chinensis,Cyperus malaccensis,and Sesuvium portulacastrum were identified.Our results showed that salt marshes are mainly distributed in Liaoning,Shandong,Jiangsu,Shanghai,and Zhejiang,with varying patterns of shrinking,expansion,or wavering in different places.The distribution of salt marshes has declined considerably from 151,324 ha in 1985 to 115,397 ha in 2019,a drop of 23.7%.During the same period,the area of native species has dropped 95.4%from 77,741 ha to 3,563 ha for Suaeda spp.and 45.1%from 60,511 ha to 33,193 ha for P.australis;on the contrary,the area of exotic species,S.alterniflora,has exhibited a sharp rise from just 99 ha to 67,527 ha.For the past 35 years,the driving factors causing salt marsh changes are mainly land reclamation,variations in water and sand fluxes,and interspecific competition and succession of salt marsh vegetation.These results provide fundamental reference information and could form the scientific basis for formulating policies for the conservation and utilization of salt marsh resources in China. | Guangwei Chen Runjie Jin Zhanjiang Ye Qi Li Jiali Gu Min Luo Yongming Luo George Christakos James Morris Junyu He Dan Li Hengwei Wang Li Song Qiuxuan Wang Jiaping Wu | 2022 | Journal of Remote Sensing2022，，1： | 2 |
| 15 | Fine mapping of qTGW10-20.8, a QTL having important contribution to grain weight variation in rice显示文摘Grain weight is one of themost important determinants of grain yield in rice.In this study,QTL analysis for grain weight,grain length,and grainwidthwas performed using populations derived from crosses between major parental lines of three-line indica hybrid rice.A total of 27 QTL for grain weight were detected using three recombinant inbred line populations derived from the crosses Teqing/IRBB lines,Zhenshan 97/Milyang 46,and Xieqingzao/Milyang 46.Of these,10 were found in only a single population and the other 17 in two or all three populations.Nine of the 17 common QTL were located in regions where no QTL associated with grain weight have been cloned and onewas selected for fine-mapping.Eight populations segregating in an isogenic background were derived from one F7 residual heterozygote of Teqing/IRBB52.The target QTL,qTGW10-20.8 controlling grain weight,grain length,and grain width,was localized to a 70.7-kb region flanked by InDel markers Te20811 and Te20882 on the long arm of chromosome 10.The QTL region contains seven annotated genes,ofwhich six encode proteins with known functional domains and one encodes a hypothetical protein.One of the genes,Os10g0536100 encoding the MIKC-type MADS-box protein OsMADS56,is the most likely candidate for qTGW10-20.8.These results provide a basis for cloning qTGW10-20.8,which has an important contribution to grain weight variation in rice. | Yujun Zhu Zhenhua Zhang Junyu Chen Yeyang Fan Tongmin Mou Shaoqing Tang Jieyun Zhuang | 2019 | The Crop Journal2019，7，5： | 2 |
| 16 | A robust soc-MOF platform exhibiting high gravimetric uptake and volumetric deliverable capacity for on-board methane storage显示文摘Emerging as an outperformed class of metal-organic frameworks(MOFs),square-octahedron(soc)topology MOFs(soc-MOFs)feature superior properties of high porosity,large gas storage capacity,and excellent thermal/chemical stability.We report here an iron based soc-MOF,denoted as Fe-pbpta(H4pbpta=4,4',4'',4'''-(1,4-phenylenbis(pyridine-4,2-6-triyl))-tetrabenzoic acid)possessing a very high Brunauer,Emmett and Teller(BET)surface area of 4,937 m2/g and a large pore volume of 2.15 cm3/g.The MOF demonstrates by far the highest gravimetric uptake of 369 cm3(STP)/g under the DOE operational storage conditions(35 bar and 298 K)and a high volumetric deliverable capacity of 192 cc/cc at 298 K and 65 bar.Furthermore,Fe-pbpta exhibits high thermal and aqueous stability making it a promising candidate for on-board methane storage. | Gaurav Verma Sanjay Kumar Harsh Vardhan Junyu Ren Zheng Niu Tony Pham Lukasz Wojtas Sydney Butikofer Jose C Echeverria Garcia Yu-Sheng Chen Brian Space and Shengqian Ma | 2021 | Nano Research2021，14，2： | 2 |
| 17 | Two newly recorded genera and a new species of Thripinae from China(Thysanoptera:Thripidae)显示文摘A new species,Bregmatothrips sinensis Wang & Tong,sp.nov.,is described and illustrated,and two species of newly recorded genera in Thripinae,Caprithrips insularis Beshear and Paithrips circularis Nonaka & Jangvitaya,are reported from China.The type materials and specimens examined are deposited in the Insect Collection,South China Agricultural University(SCAU). | Zhaohong Wang Chao Zhao Junyu Chen Xiaoli Tong | 2016 | Zoological Systematics2016，41，3： | 2 |
| 18 | Achieving a sub-10 nm nanopore array in silicon by metal-assisted chemical etching and machine learning显示文摘Solid-state nanopores with controllable pore size and morphology have huge application potential.However,it has been very challenging to process sub-10 nm silicon nanopore arrays with high efficiency and high quality at low cost.In this study,a method combining metal-assisted chemical etching and machine learning is proposed to fabricate sub-10 nm nanopore arrays on silicon wafers with various dopant types and concentrations.Through a SVM algorithm,the relationship between the nanopore structures and the fabrication conditions,including the etching solution,etching time,dopant type,and concentration,was modeled and experimentally verified.Based on this,a processing parameter window for generating regular nanopore arrays on silicon wafers with variable doping types and concentrations was obtained.The proposed machine-learning-assisted etching method will provide a feasible and economical way to process high-quality silicon nanopores,nanostructures,and devices. | Yun Chen Yanhui Chen Junyu Long Dachuang Shi Xin Chen Maoxiang Hou Jian Gao Huilong Liu Yunbo He Bi Fan Ching-Ping Wong Ni Zhao | 2021 | International Journal of Extreme Manufacturing2021，3，3： | 2 |
| 19 | Non-Structural Protein 5 of Zika Virus Interacts with p53 in Human Neural Progenitor Cells and Induces p53-Mediated Apoptosis显示文摘Zika virus(ZIKV) infection could disrupt neurogenesis and cause microcephaly in neonates by targeting neural progenitor cells(NPCs). The tumor suppressor p53-mediated cell cycle arrest and apoptotic cell death have been suggested to be activated upon ZIKV infection, yet the detailed mechanism is not well understood. In the present study, we investigated the effects of ZIKV-encoded proteins in the activation of p53 signaling pathway and found that, among the ten viral proteins,the nonstructural protein 5(NS5) of ZIKV most significantly activated the transcription of p53 target genes. Using the immunoprecipitation-coupled mass spectrometry approach, we identified that ZIKV-NS5 interacted with p53 protein. The NS5-p53 interaction was further confirmed by co-immunoprecipitation and GST pull-down assays. In addition, the MTase domain of NS5 and the C-terminal domain of p53 were mapped to be responsible for the interaction between these two proteins. We further showed that ZIKV-NS5 was colocalized with p53 and increased its protein level in the nuclei and able to prolong the half-life of p53. Furthermore, lentivirus-mediated expression of ZIKV-NS5 in hNPCs led to an apparent cell death phenotype. ZIKV-NS5 promoted the cleavage of PARP1 and significantly increased the cell apoptosis of h NPCs.Taken together, these findings revealed that ZIKV-NS5 is a previously undiscovered regulator of p53-mediated apoptosis in hNPCs, which may contribute to the ZIKV-caused abnormal neurodevelopment. | Ping Li Hualian Jiang Hong Peng Weijie Zeng Yongheng Zhong Miao He Luyang Xie Junhai Chen Deyin Guo Junyu Wu Chun-Mei Li | 2021 | Virologica Sinica2021，36，6： | 2 |
| 20 | Transcriptome analyses of insect cells to facilitate baculovirus-insect expression显示文摘五根房间线(BTI-TN-5B1-4 ) 从卷心菜 looper 孤立的高度, Trichoplusia ni 是广泛地为调停 baculovirus 的 recombinant 使用的一根昆虫房间线蛋白质表示。尽管有它在工业和学术实验室的普遍申请,这根房间线的 genomic 背景仍然保持不清楚。这里,我们定序高度五房间的 transcriptome 并且装配了 25,234 个抄本。Codon 用法分析证明高,五个房间有一个柔韧的 codon 用法能力因此为表示蛋白质适合真核细胞起源、原核生物起源。涉及 glycosylation 的基因在我们的学习被介绍,提供为设计在昆虫房间的 glycosylated 蛋白质的指导。我们也在高度五与高表示丰富为抄本预言了信号肽并且 Sf21 房间线,和这些结果为优化能分泌的一些和膜蛋白质的表示水平有重要含意。 | Kai Yu Yang Yu Xiaoyan Tang Huimin Chen Junyu Xiao Xiao-Dong Su | 2016 | Protein & Cell2016，7，5： | 2 |
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