|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 2 | Studies on mechanism of Sialy Lewis-X antigen in liver metastases of human colorectal carcinoma显示文摘INTRODUCTIONSialyl Lewis-X antigen ,correlated with carcinoma, is a group of carbohydrate antigen containing oligosaccharide expressed of embryonic tisue and glycoproteins on cell surface of embryonic tissue[1].The SLeX antigen located on cell surface is synthesized principally by two enzymes ,al ,3fucosyltransfrease and a2, 3sialyctransferase.In adults ,SLeX antigen is expressed principally on the surfaces of granulocytic cells and some tumor cells . | Xiao Wei Li~1 Yan Qing Ding~1 Jun Jie Cai~1 Shao Qing Yang~2 Lian Bing An~3 Dong Fang Qiao~3 ~1Department of Pathology,Nanfang Hospital of the First Military Medical University,Guangzhou 510515,Guangdong Province,China ~2The Northern Hospital of PLA,Shenyang 110015,Liaoning Province,China ~3Department of Electronmicroscopy,First Military Medical University,Guangzhou 510515,Gangdong Province,ChinaDr.Xiao Wei Li graduated from the First Military Medical University with a MM degree in 1999.Physician in Charge of pathology,having 6 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 19 |
| 3 | Characterization of Nestin-positive stem Leydig cells as a potential source for the treatment of testicular Leydig cell dysfunction显示文摘 | Mei Hua Jiang Bing Cai Ying Tuo Jiancheng Wang Zhi Jun Zang Xiang'an Tu Yong Gao Zhijian Su Weiqiang Li Guilan Li Min Zhang Jianwei Jiao Zi Wan Chunhua Deng Bruce T Lahn Andy Peng Xiang | 2014 | Cell Research2014，24，12： | 14 |
| 4 | Choice of approach for hepatectomy for hepatocellular carcinoma located in the caudate lobe:Isolated or combined lobectomy?显示文摘AIM:To investigate the significance of the surgical approaches in the prognosis of hepatocellular carcinoma(HCC) located in the caudate lobe with a multivariate regression analysis using a Cox proportional hazard model.METHODS:Thirty-six patients with HCC underwent caudate lobectomy at a single tertiary referral center between January 1995 and June 2010.In this series,left-sided,right-sided and bilateral approaches were used.The outcomes of patients who underwent isolated caudate lobectomy or caudate lobectomy combined with an additional partial hepatectomy were compared.The survival curves of the isolated and combined resection groups were generated by the Kaplan-Meier method and compared by a log-rank test.RESULTS:Sixteen(44.4%) of 36 patients underwent isolated total or partial caudate lobectomy whereas 20(55.6%) received a total or partial caudate lobectomy combined with an additional partial hepatectomy.The median diameter of the tumor was 6.7 cm(range,2.1-15.8 cm).Patients who underwent an isolated caudate lobectomy had significantly longer operative time(240 min vs 170 min),longer length of hospital stay(18 d vs 13 d) and more blood loss(780 mL vs 270 mL) than patients who underwent a combined caudate lobectomy(P < 0.05).There were no perioperative deaths in both groups of patients.The complication rate was higher in the patients who underwent an isolated caudate lobectomy than in those who underwent combined caudate lobectomy(31.3% vs 10.0%,P < 0.05).The 1-,3-and 5-year disease-free survival rates for the isolated caudate lobectomy and the combined caudate lobectomy groups were 54.5%,6.5% and 0% and 85.8%,37.6% and 0%,respectively(P < 0.05).The corresponding overall survival rates were 73.8%,18.5% and 0% and 93.1%,43.6% and 6.7%(P < 0.05).CONCLUSION:The caudate lobectomy combined with an additional partial hepatectomy is preferred because this approach is technically less demanding and offers an adequate surgical margin. | Peng Liu Bao-An Qiu Gang Bai Hong-Wei Bai Nian-Xin Xia Ying-Xiang Yang Jian-Yong Zhu Yang An Bing Hu | 2012 | World Journal of Gastroenterology2012，18，29： | 13 |
| 5 | Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells显示文摘RNase III DROSHA is upregulated in multiple cancers and contributes to tumor progression by hitherto unclear mechanisms.Here,we demonstrate that DROSHA interacts withβ-Catenin to transactivate STC1 in an RNA cleavage-independent manner,contributing to breast cancer stem-like cell(BCSC)properties.DROSHA mRNA stability is enhanced by N6-methyladenosine(m^(6)A)modification which is activated by AURKA in BCSCs.AURKA stabilizes METTL14 by inhibiting its ubiquitylation and degradation to promote DROSHA mRNA methylation.Moreover,binding of AURKA to DROSHA transcript further strengthens the binding of the m^(6)A reader IGF2BP2 to stabilize m^(6)A-modified DROSHA.In addition,wild-type DROSHA,but not an m^(6)A methylation-deficient mutant,enhances BCSC stemness maintenance,while inhibition of DROSHA m^(6)A modification attenuates BCSC traits.Our study unveils the AURKA-induced oncogenic m^(6)A modification as a key regulator of DROSHA in breast cancer and identifies a novel DROSHA transcriptional function in promoting the BCSC phenotype. | Fei Peng Jie Xu Bai Cui Qilan Liang Sai Zeng Bin He Hong Zou Manman Li Huan Zhao Yuting Meng Jin Chen Bing Liu Shasha Lv Peng Chu Fan An Zifeng Wang Junxiu Huang Yajing Zhan Yuwei Liao Jinxin Lu Lingzhi Xu Jin Zhang Zhaolin Su Zhiguang Li Fangjun Wang Eric W-FLam Quentin Liu | 2021 | Cell Research2021，31，3： | 9 |
| 6 | High Staphylococcus epidermidis Colonization and Impaired Permeability Barrier in Facial Seborrheic Dermatitis显示文摘 | Qian An Meng Sun Rui-Qun Qi Li Zhang Jin-Long Zhai Yu-Xiao Hong Bing Song Hong-Duo Chen Xing-Hua Gao | 2017 | Chinese Medical Journal2017，，14： | 8 |
| 7 | Facile aqueous synthesis and thermal insulating properties of low-density glass/TiO_2 core/shell composite hollow spheres显示文摘Anatase TiO2 shells assembled on hollow glass microspheres(HGM)with tunable morphologies were successfully prepared through a controllable chemical precipitation method with urea as the precipitator. Thus,glass/TiO2 core/shell composite hollow spheres with low particle density(0.40 g/cm3)were fabricated.The phase structures,morphologies,particle sizes,shell thicknesses,and chemical compositions of the composite microspheres were characterized by X-ray diffraction(XRD),scanning electron microscopy (SEM),and energy dispersive X-ray spectroscopy(EDS).The morphology of the TiO2 shell can be tailored by properly monitoring the reaction system component and parameters.The probable growth mechanism and fabrication process of the core/shell products involving the nucleation and oriented growth of TiO2 nanocrystals on hollow glass microspheres was proposed.A low infrared radiation study revealed that the radiation properties of the products are greatly influenced by the unique product shell structures. A thermal conductivity study showed that the TiO2/HGM possess low thermal conductivity that is similar to that of the pristine HGMs.This work provides an additional strategy to prepare low-density thermal insulating particles with tailored morphologies and properties. | Jing Yuan Zhenguo An Bing Li Jingjie Zhang | 2012 | Particuology2012，10，4： | 8 |
| 8 | Retrograde approach for the recanalization of coronary chronic total occlusion: preliminary experience of a single center显示文摘为开运河不戏剧性地有的长期的全部的吸藏(CTO ) 的 antegrade 途径的成功率增加了的背景,特别在复杂 CTO 子集。retrograde 技术可以保持大诺言。这份报告试图描述我们的经验后退为 CTO 的开运河,集中于它的安全和可行性。我们识别了在 CTO 经历了 revascularization 与的 42 个病人的方法后退在我们的 center.Results 的 2009 年 11 月的从 2005 年 7 月的途径三种策略被使用:作为主要策略(50.0%) 后退,在 antegrade 失败(26.2%) 以后立即后退并且重复在以前的 antegrade 失败(23.8%) 以后的过程。氏族的担保更经常被用作 retrograde 存取线路(92.9%) 。全面成功率是 88.1% 。在病人与成功后退电线十字路口担保隧道到 CTO 的远侧的帽子,开运河的成功率是 94.1% 。在有失败到的病人穿过担保,成功率是 62.5% 。八种不同类型后退技术被使用:吻的电线技术(35.3%),套住的电线和套住的反向的电线技术(17.6%),后端汽球和 microcatherer 颠倒技术(14.7%),控制 antegrade 并且后退追踪的 subintimal (大车)技术(8.8%),反向的大车和修改颠倒运技术(8.8%),后退电线十字路口技术(2.9%)。有 4 复杂并发症没有在里面医院,发生了主要不利心脏的事件(向) 。在里面医院向是 7.7% 。所有他们是 non-Q 挥动心肌的梗塞。没有死亡的盒子或指向 retrograde 途径能是的容器 revascularization,任何一个外科或 percutaneous.Conclusions 为在复杂 CTO 增加开运河的成功率的一个有效工具。保证由富有经验的操作员处理的途径,小心的盒子选择和设备的成功和安全是必要的。 | GE Lei QIAN Ju-ying LIU Xue-bo QIN Qing CUI Shan-jing YAO Kang SHEN Li MA Jian-ying HUANG Dong XU Shi-kun ZHANG Feng WANG Xiang-fei WANG Qi-bing FAN Bing YAN Yan FENG Qi WANG Hao SHEN An ZHU Ming-hui GE Jun-bo | 2010 | Chinese Medical Journal2010，，7： | 7 |
| 9 | Transition from Metastablity to Instability in Dynamics for the Precipitation of δ'(Al_3Li)显示文摘A computer simulation study on dynamics for the precipitation of δ’(Al3Li) ordered particles from a disor- dered matrix (α) in binary Al-Li alloys is performed using the microscopic Langevin equation. A unique precipitation mechanism is found near the ordering spinodal line. Different from the classical nucleation mechanism in the me- tastable region and the congruent ordering followed by spinodal decomposition in the instable region, a nonstoichi- ometric single ordered phase with composition fluctuations is formed by non-classical nucleation, and this ordered phase decomposes spinodally. It can be concluded that the precipitation dynamics of δ’ phase from metastability to instability is gradual, and no sharp transition occurs near the mean-field spinodal line as the mean-field theory pre- dicts. | Xiaoling Li, Zheng Chen, Bing Liu, Yongxin Wang Department of Materials Science and Engineering, Northwestern Polytechnical University. Xi’an, 710072, China | 2001 | Rare Metals2001，20，4： | 7 |
| 10 | Preparation and Performance of Salt Tolerance and Thermal Stability Cellulose Nanofibril Hydrogels and Their Application in Drilling Engineering显示文摘The poor salt tolerance,thermal stability,and environmental performance of petrochemicals can severely limit their applications in drilling engineering.In this study,cellulose nanofibril(CNF)hydrogels with improved salt tolerance and thermal stability were prepared,and their filtration performance was evaluated.The hydrogels were prepared through the simultaneous grafting of 2-acrylamido-2-methylpropane sulfonic acid(AMPS)and butyl acrylate(BA)onto the CNF surface through ceric ammoniumnitrate-induced radical polymerization.The modified and original CNF samples were characterized using Fourier Transform infrared spectroscopy(FT-IR)and rheological measurements.The FT-IR analysis results showed that both AMPS and BA were grafted onto the CNF backbone,affirming the successful preparation of the grafted CNFs.The rheological analysis results showed that the modified CNF hydrogels exhibited significantly improved salt tolerance,thermal stability,and“salt-thickening”effect.Moreover,the results of the fluid loss test showed that the modified CNF hydrogels exhibited a much better fluid loss control than the original CNF hydrogels.In addition,after adding 2%modified CNF hydrogels as a filtrate reducer in the drilling fluids prepared with a 6%combined salt solution,the filtrate loss was significantly reduced even after aging for 72 h at 160℃. | XiongLi Liu An Wang ChunPing Wang JiaLei Qu YangBing Wen Bin Chen ZhongGuang Wang BinBin Wu ZhaoYang Yuan Bing Wei | 2019 | Paper And Biomaterials2019，4，2： | 7 |
| 11 | Percutaneous extraction of leads from coronary sinus vein and branch by modified techniques显示文摘 | CHU Xian-ming LI Xue-bin ZHANG Ping, WANG Long LI Ding LI Bing AN Yi LENG Min DUAN Jiang-bo GUO Ji-hong | 2012 | Chinese Medical Journal2012，，20： | 6 |
| 12 | The heterogeneity of islet autoantibodies and the progression of islet failure in type 1 diabetic patients显示文摘Type 1 diabetes mellitus is heterogeneous in many facets. The patients suffered from type 1 diabetes present several levels of islet function as well as variable number and type of islet-specific autoantibodies. This study was to investigate prevalence and heterogeneity of the islet autoantibodies and clinical phenotypes of type 1 diabetes mellitus; and also discussed the process of islet failure and its risk factors in Chinese type 1 diabetic patients. A total of 1,291 type 1 diabetic patients were enrolled in this study. Demographic information was collected. Laboratory tests including mixed-meal tolerance test, human leukocyte antigen alleles, hemoglobin A1 c, lipids, thyroid function and islet autoantibodies were conducted. The frequency of islet-specific autoantibody in newly diagnosed T1 DM patients(duration shorter than half year) was 73% in East China. According to binary logistic regressions, autoantibody positivity, longer duration and lower Body Mass Index were the risk factors of islet failure. As the disease developed, autoantibodies against glutamic acid decarboxylase declined as well as the other two autoantibodies against zinc transporter 8 and islet antigen 2. The decrease of autoantibodies was positively correlated with aggressive beta cell destruction. Autoantibodies can facilitate the identification of classic T1 DM from other subtypes and predict the progression of islet failure. As there were obvious heterogeneity in autoantibodies and clinical manifestation in different phenotypes of the disease, we should take more factors into consideration when identifying type 1 diabetes mellitus. | Jin Liu Lingling Bian Li Ji Yang Chen Heng Chen Yong Gu Bingqin Ma Wei Gu Xinyu Xu Yun Shi Jian Wang Dalong Zhu Zilin Sun Jianhua Ma Hui Jin Xing Shi Heng Miao Bing Xin Yan Zhu Zhenwen Zhang Ruifang Bu Lan Xu Guangde Shi Wei Tang Wei Li Dongmei Zhou Jun Liang Xingbo Cheng Bimin Shi Jixiang Dong Ji Hu Chen Fang Shao Zhong Weinan Yu Weiping Lu Chenguang Wu Li Qian Jiancheng Yu Jialin Gao Xiaoqiang Fei Qingqing Zhang Xueqin Wang Shiwei Cui Jinluo Cheng Ning Xu Guofeng Wang Guoqing Han Chunrong Xu Yun Xie Minmin An Wei Zhang Zhixiao Wang Yun Cai Qi Fu Yu Fu Shuai Zheng Fan Yang Qingfang Hu Hao Dai Yu Jin Zheng Zhang Kuanfeng Xu Yifan Li Jie Shen Hongwen Zhou Wei He Xuqin Zheng Xiao Han Liping Yu Jinxiong She Mei Zhang Tao Yang | 2016 | Science China(Life Sciences)2016，59，9： | 5 |
| 13 | An Effective Over-sampling Method for Imbalanced Data Sets Classification显示文摘 | ZHAI Yun MA Nan RUAN Da AN Bing | 2011 | Chinese Journal of Electronics2011，20，3： | 5 |
| 14 | Impacts of Uncertainty in AVOC Emissions on the Summer RO_x Budget and Ozone Production Rate in the Three Most Rapidly-Developing Economic Growth Regions of China显示文摘High levels of uncertainty in non-methane volatile organic compound(NMVOC) emissions in China could lead to significant variation in the budget of the sum of hydroxyl(OH) and peroxy(HO2, RO2) radicals(ROx= OH + HO2+ RO2)and the ozone production rate [P(O3)], but few studies have investigated this possibility, particularly with three-dimensional air quality models. We added diagnostic variables into the WRF-Chem model to assess the impact of the uncertainty in anthropogenic NMVOC(AVOC) emissions on the ROxbudget and P(O3) in the Beijing–Tianjin–Hebei region, Yangtze River Delta, and Pearl River Delta of China. The WRF-Chem simulations were compared with satellite and ground observations,and previous observation-based model studies. Results indicated that 68% increases(decreases) in AVOC emissions produced4%–280% increases(2%–80% decreases) in the concentrations of OH, HO2, and RO2 in the three regions, and resulted in35%–48% enhancements(26%–39% reductions) in the primary ROxproduction and ~ 65% decreases(68%–73% increases)of the P(O3) in Beijing, Shanghai, and Guangzhou. For the three cities, the two largest contributors to the ROxproduction rate were the reaction of O1 D + H2 O and photolysis of HCHO, ALD2, and others; the reaction of OH + NO2(71%–85%)was the major ROxsink; and the major contributor to P(O3) was the reaction of HO2+ NO(~ 65%). Our results showed that AVOC emissions in 2006 from Zhang et al.(2009) have been underestimated by ~ 68% in suburban areas and by > 68%in urban areas, implying that daily and hourly concentrations of secondary organic aerosols and inorganic aerosols could be substantially underestimated, and cloud condensation nuclei could be underestimated, whereas local and regional radiation was overestimated. | WANG Feng AN Junling LI Ying TANG Yujia LIN Jian QU Yu CHEN Yong ZHANG Bing ZHAI Jing | 2014 | Advances in Atmospheric Sciences2014，31，6： | 5 |
| 15 | P ill C am colon capsule endoscopy versus conventional colonoscopy for the detection of severity and extent of ulcerative colitis显示文摘 | Chen An Ye Yun Jie Gao Zhi Zheng Ge Jun Dai Xiao Bo Li Han Bing Xue Zhi Hua Ran Yun Jia Zhao | 2013 | Journal of Digestive Diseases2013，，3： | 4 |
| 16 | Application of UPLC-MS/MS Method for Analyzing B-vitamins in Human Milk显示文摘Objective To determine ten B-vitamins in human milk by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS). Methods The pretreated human milk samples were adequately separated and quantified within 11 min by UPLC-MS/MS with an Acquity UPLC HSS T3 column(2.1×100 mm, 1.8 μm). The mobile phase was a gradient of 2.5 mmol/L ammonium formate aqueous solution and acetonitrile at a flow rate of 0.35 m L/min. Stable isotope internal standards were used in the analysis, to correct for the method variability, including matrix and ionization effects. The homogenized human milk samples were deproteinzed using methanol, unknown contaminants were extracted with diethyl ether and hydrophobic phase was discarded. The analytes were monitored via ESI+ionization and detected in multiple reaction monitoring(MRM) with three acquisition functions. Results Calibration curves ranged from 0.5-160 ng/m L(thiamin, riboflavin, biotin, nicotinic acid, pyridoxine, pyridoxamine, pyridoxal), and 2.5-800 ng/m L(pantothenic acid, FAD and nicotinamide)(R2=0.990-0.999). The relative recovery ranged from 80.1% to 120.2%; accuracy was determined to be 98.3% to 108.0%. Intra-day and inter-day variation were 3.4%-19.9% and 5.9%-18.1%, respectively. The limit of quantification(LOQ) for all vitamins was between 0.25 and 3 μg/L. Conclusion This method was successfully applied for simultaneous analysis of ten B-vitamins in human milk. | REN Xiang Nan YIN Shi An YANG Zhen Yu YANG Xiao Guang SHAO Bing REN Yi Ping ZHANG Jing | 2015 | Biomedical and Environmental Sciences2015，28，10： | 4 |
| 17 | Prediction of daytime variations of HO_2 radical concentrations in the marine boundary layer using BP network显示文摘A Back-Propagation Neural Network (BPNN) was established to predict the daytime variations of HO 2 radical concentration observed in the field campaign RISFEX 2003 (RIShiri Fall Experiment 2003) conducted in September 2003 at Rishiri Island (45.07 N,141.12 E,and 35m asl) in the Sea of Japan.The initial weight matrices and bias vectors for the network were optimized by a bee evolutionary genetic algorithm (BEGA).It was found that the input variables sensitive to HO 2 variation were photolysis frequency of O 3 to O(1 D) (J(O 1 D)),a composite parameter defined as the ratio of HC to NO x reactivity towards OH radicals (Φ),and the total aerosol surface area (A).The predicted results are closely correlated with the experimental data with the coefficient of determination (R2) close to 1.In addition,the means and ranges of the predicted HO 2 concentration agree with the observed data with the correlation coefficient (R),the index of agreement (IA) and the fractional bias (FB) in the range of 0.84-0.93,0.88-0.95 and 14%-7%,respectively.This study demonstrates that BPNN is a potential tool to predict the daytime variations of HO 2 radical concentrations in the marine boundary layer (MBL). | WANG ZhuQing,CHEN YaShao,QI Bin & YANG Bing Key laboratory of Applied Surface and Colloid Chemistry,Ministry of Education School of Chemistry and Material Science,Shaanxi Normal University,Xi’an 710062,China | 2010 | Science China Chemistry2010，53，12： | 4 |
| 18 | Expressions of Nucleoside Diphosphate Kinase (nm23) in Tumor Tissues are Related with Metastasis and Length of Survival of Patients with Hepatocellular Carcinoma显示文摘Objective To evaluate the relationship of expressions of nucleoside diphosphate kinase (nm23) and proliferating cell nuclear antigen (PCNA), as well as apoptosis, with the prognosis of HCC patients by analyzing their pathological and clinical data. Methods The expressions of nm23 and PCNA were analyzed by immunohistochemistry and the apoptotic phenomena were detected by TUNEL technique in the liver samples from 43 HCC tissues, 39 para-neoplastic tissues, and 10 normal tissues. The mean apoptosis index (AI) and proliferative index (PI) in individual sample were calculated. Results As shown by the detection, 32.6% of carcinomas had negative nm23 signal in tumor tissues, whereas all para-neoplastic and normal tissues had positive nm23. The AI in nm23 positive HCC was significantly higher than that in nm23 negative one, with statistical difference (P<0.05). Furthermore, the expressions of nm23, and the values of AI and PI were contrastively analyzed with some main pathological and clinical data of HCC. It revealed that HCC with extrahepatic metastasis showed remarkable correlation with the negative nm23 (P=0.013) and higher PI values of HCC (P=0.015). The disease-free survival in HCC patients with negative nm23 expression was significantly poorer than that in patients with positive nm23 expression. Conclusion These data suggest that expressions of nm23 protein in tumor tissues are correlated with occurrences of metastasis and length of survival of the HCC patients, which may be an indicator for their prognosis. | RUN AN JIE MENG QI SHI XIAO-XIA DAI JING-HONG CHEN YAN-JUN LEI BING SHAN CHEN GAO YONG-LIE CHU XIAO-PING DONG | 2010 | Biomedical and Environmental Sciences2010，23，4： | 4 |
| 19 | Establishment of a Sandwich ELISA Method for Detection of Vascular Endothelial Growth Factor in Serum Samples of Hepatocellular Carcinoma Patients显示文摘Objective To establish a sandwich ELISA method for detecting vascular endothelial growth factor (VEGF) in sera of population and the patients with hepatocellular carcinoma (HCC). Methods Full length and two truncated human VEGF cDNA sequences were amplified from a commercial plasmid pBLAST49-hVEGF by PCR and inserted into the prokaryotic-expression plasmid pET-32a or pGEX-2T. Various VEGF proteins were expressed and purified from E. coli in His-Trx or GST fusion forms. The specific VEGF antibodies were elicited in experimental rabbits and mice by immunization of the full length VEGF fusion protein His-Trx-VEGF1-165. After purification of antibodies with chromatograph of Protein G, a sandwich ELISA technique was established. Serum VEGF levels were evaluated in 229 adults and 291 HCC patients. Results SDS-PAGE displayed that the molecular weights of the expressed full length (His-Trx-VEGF1-165), N-terminal (His-Trx-VEGF1-100) and C-terminal (GST-VEGF100-165) human VEGF fusion proteins were about 38KD, 31KD, and 33KD, respectively. Western blots confirmed that the prepared antisera were able to recognize both prokaryoticly and eukaryoticly expressed recombinant VEGF proteins. Assays of serially diluted His-Trx-VEGF1-100 by the established sandwich ELISA method showed that the linear range of the standard curve was 0.625-320 ng/mL, with the squared correlation coefficient R2=0.991. Screening of a serum panel containing 291 serum samples of HCC patients and 229 health adults revealed that the average VEGF level in HCC patients was higher than that in healthy controls, with a statically significant difference. Conclusion The established sandwich ELISA reflects the level of serum VEGF and provide scientific basis for screening metastasis and recurrence of HCC using serum VEGF as an index. | BING SHAN CHEN GAO JIAN-MING CHEN XIN-Yu BI BAO-YUN ZHANG YAN GUO CHEN-FANG DONG RUN AN QI SHI JING-QUN HU PING ZHAO JUN HAN XIAO-PING DONG | 2008 | Biomedical and Environmental Sciences2008，21，1： | 4 |
| 20 | Progress of Jinping Underground laboratory for Nuclear Astrophysics(JUNA)显示文摘Jinping Underground laboratory for Nuclear Astrophysics(JUNA) will take the advantage of the ultra-low background of CJPL lab and high current accelerator based on an ECR source and a highly sensitive detector to directly study for the first time a number of crucial reactions occurring at their relevant stellar energies during the evolution of hydrostatic stars. In its first phase, JUNA aims at the direct measurements of^(25)Mg(p,γ)^(26)Al,^(19)F(p,α)^(16)O,^(13)C(α,n)^(16)O and ^(12)C(α,γ)^(16)O reactions. The experimental setup,which includes an accelerator system with high stability and high intensity, a detector system, and a shielding material with low background, will be established during the above research. The current progress of JUNA will be given. | WeiPing Liu ZhiHong Li JiangJun He XiaoDong Tang Gang Lian Zhu An JianJun Chang Han Chen QingHao Chen XiongJun Chen ZhiJun Chen BaoQun Cui XianChao Du ChangBo Fu Lin Gan Bing Guo GuoZhu He Alexander Heger SuQing Hou HanXiong Huang Ning Huang BaoLu Jia LiYang Jiang Shigeru Kubono JianMin Li KuoAng Li Tao Li YunJu Li Maria Lugaro XiaoBing Luo HongYi Ma ShaoBo Ma DongMing Mei YongZhong Qian JiuChang Qin Jie Ren YangPing Shen Jun Su LiangTing Sun WanPeng Tan Isao Tanihata Shuo Wang Peng Wang YouBao Wang Qi Wu ShiWei Xu ShengQuan Yan LiTao Yang Yao Yang XiangQing Yu Qian Yue Sheng Zeng HuanYu Zhang Hui Zhang LiYong Zhang NingTao Zhang QiWei Zhang Tao Zhang XiaoPeng Zhang XueZhen Zhang ZiMing Zhang Wei Zhao Zuo Zhao Chao Zhou | 2016 | Science China(Physics,Mechanics & Astronomy)2016，59，4： | 3 |
您所在的机构暂未开通此部分的功能，请联系管理员