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| 1 | Global Analysis of Direct Targets of Secondary Wall NAC Master Switches in Arabidopsis显示文摘我们报导 SND1 和 VND7 的直接目标基因的染色体宽的分析,分别地是在纤维和容器的第二等的墙生合成的主人管理者的二个 Arabidopsis thaliana NAC 领域抄写因素。使用 electrophoretic 活动性移动试金和 transactivation 分析的 SND1 有约束力的顺序的系统的印射和另外的第二等的墙 NAC ( SWN )表明了那 SND1 ,包括 VND6 , VND7 , NST1 ,和 NST2 ,绑在指定为第二等的墙 NAC 有约束力的元素( SNBE )的一个有瑕疵的 palindromic 19-bp 一致序列,( T/A ) NN ( C/T )( T/C/G ) TNNNNNNNA (往来帐户) GN ( A/C/T )( A/T ),在他们的直接目标的倡导者。SND1 和 VND7 的直接目标的染色体宽的分析表明他们直接激活不仅下游的抄写因素,而且涉及第二等的墙生合成,房间墙修正,和规划房间死亡,其倡导者都包含多重 SNBE 地点的很多非抄写因素基因的表示。SND1 和 VND7 直接调整一套普通目标的表示但是他们中的每优先地也导致直接目标的一个不同集合,它多半向 SNBE 地点被归因于他们的微分激活力量。互补学习证明 SWN 能在 snd1 nst1 异种救第二等的墙缺点,显示他们是机能上地可互换的。一起,我们的结果提供重要卓见进复杂 transcriptional 节目和在第二等的包含墙的房间类型位于第二等的墙生合成,房间墙修正,和规划房间死亡下面的进化机制。 | Ruiqin Zhong Chanhui Lee Zheng-Hua Ye | 2010 | Molecular Plant2010，3，6： | 38 |
| 2 | Arabidopsis Galacturonosyltransferase (GAUT) 13 and GAUT14 Have Redundant Functions in Pollen Tube Growth显示文摘房间墙生合成为花粉试管生长是不可缺少的。尽管有它到性复制的重要性,花粉试管墙生合成遗体的 molecularmechanisms 糟糕理解。这里,我们报导二 putativeArabidopsis galacturonosyltransferase 基因, GAUT13 和 GAUT14 的功能的描述,它为花粉试管生长是必要的。GAUT13 和 GAUT14 编码分享高氨基酸顺序身份的蛋白质并且位于 Golgi 仪器。T-DNA insertionmutants, gaut13 和 gaut14,没展出任何看得见的缺点,而是两倍异种是的 gaut13 gaut14 在花粉试管生长有缺点;在异质接合的双异种的 35.237.3% 花粉试管是在延伸有缺点的 swollenand。房间墙的外部层没在双变异的 pollentubes 显得显然纤丝状。而且, homogalacturonan 的分发在 wassignificantly 改变与相比的双变异的花粉试管墙中用 JIM5 和 JIM7 标记野类型。我们的结果在花粉 tubegrowth 冗余地建议那 GAUT13 和 GAUT14 功能,可能通过在花粉试管 wall.Key 词的果胶生合成的参予: | Li Wang Wei Wang You-Qun Wang Yang-Yang Liu Jing-Xin Wang Xue-Qin Zhang De Ye Li-Qun Chen | 2013 | Molecular Plant2013，6，4： | 8 |
| 3 | 大豆GATL基因家族结构、进化和表达分析显示文摘本研究利用同源检索的方法在大豆数据库中全基因组分析获得18个大豆GATL基因,大豆GATL基因的基因结构保守,其中16个成员不含内含子;其后对其编码蛋白的理化特点、亚细胞定位和信号肽进行了分析;通过邻接法构建了该家族的进化树,以了解GATL家族成员的进化关系;表达模式分析发现,家族成员呈现出各自的表达特点;成员启动子区顺式作用元件分析发现了大量的植物激素和胁迫响应相关元件。本研究为进一步分析基因家族成员的功能提供了理论依据。 | 刘金龙 李绍波 | 2017 | 基因组学与应用生物学2017，36，2： | 1 |
| 4 | LEUNIG_HOMOLOG and LEUNIG Regulate Seed Mucilage Extrusion in Arabidopsis显示文摘LEUNIG(LUG)and LEUNIG_HOMOLOG(LUH)encode two closely related Arabidopsis proteins,belonging to the Gro/TLE family of transcriptional co-repressors.These two genes were previously shown to exhibit partially overlapping functions in embryo and flower development.In this report,the role of both LUH and LUG on seed mucilage extrusion was examined.Seed mucilage extrusion occurs after the seeds are imbibed,serving as functional aid in seed hydration,germination,and dispersal.While luh-1 mutants exhibited strong defects in seed mucilage extrusion,lug-3 mutants exhibited a minor phenotype in mucilage extrusion.Further characterization indicates that luh-1 does not exhibit any obvious defect in seed epidermal cell differentiation,mucilage synthesis,or mucilage deposition,suggesting a specific role of LUH in mucilage extrusion.This seed mucilage phenotype of luh-1 is identical to that of mucilage modified 2(mum2)mutants.MUM2 encodes a β-galactosidase required for the modification of the mucilage.Quantitative reverse transcription polymerase chain reaction of RNA extracted from siliques detected a slight decrease of MUM2 mRNA in the luh-1 mutant compared to the wild type.Together,LUH and possibly LUG may specifically regulate mucilage extrusion by promoting the expression of genes required for mucilage maturation. | Minh Bui Nathan Lim Paja Sijacic | 2011 | Journal of Integrative Plant Biology2011，53，5： | 1 |
| 5 | Evolution of pectin synthesis relevant galacturonosyltransferase gene family and its expression during cotton fiber development显示文摘Background:Pectin is a key substance involved in cell wall development,and the galacturonosyltransferases(GAUTs)gene family is a critical participant in the pectin synthesis pathway.Systematic and comprehensive research on GAUTs has not been performed in cotton.Analysis of the evolution and expression patterns of the GAUT gene family in different cotton species is needed to in crease kno wledge of the functi on of pectin in cotto n fiber development.Results:In this study,we have identified 131 GAUT genes in the genomes of four Gossypium species(G.raimondii,G barbadense,G.hirsutum,and G.arboreum),and classified them as GAUT-A,GAUT-B and GAUT-C,which coding probable galacturonosyltransferases.Among them,the GAUT genes encode proteins GAUT1 to GAUT15.All GAUT proteins except for GAUT7 contai n a con served glycosyl transferase family 8 domain(H-DN-A-SW-S-V-H-T-F).The conserved sequence of GAUT7 is PLN(phospholamban)02769 domain.According to c/s-elemet analysis,GAUT genes transcript levels may be regulated by horm ones such as JA,GA,SA,ABA,Me-JA,and IA A.The evoluti on and transcription patterns of the GAUT gene family in different cotton species and the transcript levels in upland cotton lines with different fiber st「ength were analyzed.Peak transcript level of GhGAUT genes have been observed before 15 DPA.In the six materials with high fiber strength,the transcription of GhGAUT genes were concentrated from 10 to 15 DPA;while the highest transcript levels in low fiber st「ength materials were detected between 5 and 10 DPA.These results lays the foundation for future research on gene function during cotton fiber development.Conclusions:The GAUT gene family may affect cotton fiber development,including fiber elongation and fiber thickening.In the low strength fiber lines,GAUTs mainly participate in fiber elongation,whereas their major effect on cotton with high strength fiber is related to both elongation and thickening. | FAN Senmiao LIU Aiying ZOU Xianyan ZHANG Zhen GE Qun GONG Wankui LI Junwen GONG Juwu SHI Yuzhen DENG Xiaoying JIA Tingting YUAN Youlu SHANG Haihong | 2021 | Journal of Cotton Research2021，4，3： | 0 |
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