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| 1 | Modified hyaluronic acid hydrogels with chemical groups that facilitate adhesion to host tissues enhance cartilage regeneration显示文摘Stable integration of hydrogel implants with host tissues is of critical importance to cartilage tissue engineering.Designing and fabricating hydrogels with high adhesive strength,stability and regeneration potential are major challenges to be overcome.This study fabricated injectable adhesive hyaluronic acid(HA)hydrogel modified by aldehyde groups and methacrylate(AHAMA)on the polysaccharide backbone with multiple anchoring mechanisms(amide bond through the dynamic Schiff base reaction,hydrogen bond and physical interpenetration).AHAMA hydrogel exhibited significantly improved durability and stability within a humid environment(at least 7 days),together with higher adhesive strength(43 KPa to skin and 52 KPa to glass),as compared to commercial fibrin glue(nearly 10 KPa)and HAMA hydrogel(nearly 20 KPa).The results showed that AHAMA hydrogel was biocompatible and could be easily and rapidly prepared in situ.In vitro cell culture experiments showed that AHAMA hydrogel could enhance proliferation(1.2-folds after 3 days)and migration(1.5-folds after 12 h)of bone marrow stem cells(BMSCs),as compared to cells cultured in a culture dish.Furthermore,in a rat osteochondral defect model,implanted AHAMA hydrogel significantly promoted integration between neo-cartilage and host tissues,and significantly improved cartilage regeneration(modified O’Driscoll histological scores of 16.0±4.1 and 18.3±4.6 after 4 and 12-weeks of post-implantation in AHAMA groups respectively,12.0±2.7 and 12.2±2.8 respectively in HAMA groups,9.8±2.4 and 11.5±2.1 respectively in untreated groups).Hence,AHAMA hydrogel is a promising adhesive biomaterial for clinical cartilage regeneration and other biomedical applications. | Jiaqing Chen Jiabei Yang Li Wang Xuewei Zhang Boon Chin Heng Dong-An Wang Zigang Ge | 2021 | Bioactive Materials2021，6，6： | 6 |
| 2 | Heterogeneity of aberrant immunoglobulin expression in cancer cells显示文摘Accumulating evidence has shown that immunoglobulin(Ig)is‘unexpectedly’expressed by epithelial cancer cells and that it can promote tumor growth.The main purpose of this study was to explore the components of the cancerous Ig and its possible function.The presence of cancerous Ig in the Golgi apparatus was confirmed by immunofluorescence,indirectly suggesting that the cancerous Ig was processed and packaged in cancer cells.Western blot analysis and ELISA results indicated that cancer cells produced membrane Ig and secreted Ig into the supernatant fraction.The cancerous Ig consists of an a heavy chain and a k light chain.Finally,by analyzing the Ig components pulled down by protein A beads,the cancerous Ig was found to be structurally distinct from normal Ig.The cancerous Ig was truncated or aberrant.Although the underlying mechanism that causes the abnormalities has not been determined,our current discoveries strengthen our previous findings and promise fruitful future explorations. | Duosha Hu Zhi Duan Ming Li Yiqun Jiang Haidan Liu Hui Zheng Lili Li Ann M Bode Zigang Dong Ya Cao | 2011 | Cellular & Molecular Immunology2011，8，6： | 5 |
| 3 | A novel inhibitor of N^(6)-methyladenosine demethylase FTO induces m RNA methylation and shows anti-cancer activities显示文摘N^(6)-methyladenosine(m^(6)A)modification is critical for m RNA splicing,nuclear export,stability and translation.Fat mass and obesity-associated protein(FTO),the first identified m^(6)A demethylase,is critical for cancer progression.Herein,we developed small-molecule inhibitors of FTO by virtual screening,structural optimization,and bioassay.As a result,two FTO inhibitors namely 18077 and 18097 were identified,which can selectively inhibit demethylase activity of FTO.Specifically,18097 bound to the active site of FTO and then inhibited cell cycle process and migration of cancer cells.In addition,18097 reprogrammed the epi-transcriptome of breast cancer cells,particularly for genes related to P53 pathway.18097 increased the abundance of m^(6)A modification of suppressor of cytokine signaling1(SOCS1)m RNA,which recruited IGF2 BP1 to increase m RNA stability of SOCS1 and subsequently activated the P53 signaling pathway.Further,18097 suppressed cellular lipogenesis via downregulation of peroxisome proliferator-activated receptor gamma(PPARγ),CCAAT/enhancer-binding protein alpha(C/EBPa),and C/EBPβ.Animal studies confirmed that 18097 can significantly suppress in vivo growth and lung colonization of breast cancer cells.Collectively,we identified that FTO can work as a potential drug target and the small-molecule inhibitor 18097 can serve as a potential agent against breast cancer. | Guoyou Xie Xu-Nian Wu Yuyi Ling Yalan Rui Deyan Wu Jiawang Zhou Jiexin Li Shuibin Lin Qin Peng Zigang Li Hongsheng Wang Hai-Bin Luo | 2022 | Acta Pharmaceutica Sinica B2022，12，2： | 4 |
| 4 | Promotion of cell proliferation and inhibition of ADCC by cancerous immunoglobulin expressed in cancer cell lines显示文摘To explore the significance of cancerous immunoglobulin(Ig)in cancer cell growth,HeLa cervical cancer cells were stably transfected with small interfering RNA(siRNA)that specifically,efficiently and consistently silences the expression of heavy chain genes of all immunoglobulin isotypes.This stable cell line was used to examine cell viability,colony formation and tumor growth in athymic nude mice.The results of these experiments indicated that siRNA-mediated knockdown of cancerous Ig inhibited cell growth in vitro and suppressed tumor cell growth in immune-deficient nude mice in vivo.Similarly,this siRNA also inhibited the growth of MGC gastric cancer cells and MCF-7 breast cancer cells.Furthermore,the presence of cancerous Ig specifically reduced antibody-dependent cell-mediated cytotoxicity(ADCC)induced by an anti-human epithelial growth factor receptor(EGFR)antibody in a dose-dependent manner,suggesting that the cancerous Ig-Fc receptor interaction inhibits natural killer cell(or NK cell)effector function.The prevalent expression of Ig in human carcinomas and its capacity to promote growth and inhibit immunity might have important implications in growth regulation and targeted therapy for human cancers. | Ming Li Hui Zheng Zhi Duan Haidan Liu Duosha Hu Ann Bode Zigang Dong Ya Cao | 2012 | Cellular & Molecular Immunology2012，9，1： | 4 |
| 5 | Activation of the Ig la I promoter by the transcription factor Ets-1 triggers Ig lal-Cal germline transcription in epithelial cancer cells显示文摘 | Zhi Duan Hui Zheng San Xu Yiqun Jiang Haidan Liu Ming Li Duosha Hu Wei Li Ann M. Bode Zigang Dong Ya Cao | 2014 | Cellular & Molecular Immunology2014，11，2： | 3 |
| 6 | Two-Phased Method for Detecting Evasive Network Attack Channels显示文摘With the rapid developments of information technology,various industries become much more dependent on networks.Driven by economic interests and the game between countries reflected by growing cyberspace confrontations,evasive network attacks on information infrastructures with high-tech,high concealment and longterm sustainability become severe threats to national security.In this paper,we propose a novel two-phased method for the detection of evasive network attacks which exploit or pretend to be common legal encryption services in order to escape security inspection.Malicious communications which camouflage themselves as legal encryption application are identified in the SSL'session structure verification phase firstly,and then by serverside X.509 certificate based anomaly detection,suspicious attack behaviors are further distinguished effectively.Experiment results show that our method is very useful for detecting the network activities of certain unknown threats or new malwares.Besides,the proposed method can be applied to other similar services easily. | CAO Zigang XIONG Gang ZHAO Yong GUO Li FANG Binxing | 2014 | China Communications2014，11，8： | 2 |
| 7 | Computer Simulation of Thermal-Mechanical Processing显示文摘Supported by the high-speed SGI engineering workstation and the general MARCTM software, a system is developed to simulate the hot deformation process and the cooling process after deformation in the forging of Cr12 steel on the basis of elastic-plastic FEM techniques with coupled thermal-mechanical-microstructural alteration. In this system, a mathematical model, obtained by plentiful physical simulation experiments on Gleeble 1500 thermomechanical simulator, is introduced to describe the changes in microstructure and properties of steels. This system is proved to be reliable to simulate the thermal field and stress field as well as the microstructure of Cr12 steel during the forging process. It can also be used to optimize and control the forging process. | Ruiheng Wu, Zigang Li, Hongbing Chang, T. Y. Hsu (Zuyao Xu) and Xueyu Ruan 1.Department of Plasticity Technology, Shanghai Jiao Tong University, Shanghai 200030, China 2.Department of Metallurgical Engineering, Shanghai Technical College of Metallurgy, | 2000 | Journal of Shanghai Jiaotong university(Science)2000，5，1： | 2 |
| 8 | Postconditioning of stellate ganglion block improves intestinal barrier function by inhibiting autophagy in conscious rats following hemorrhagic shock and resuscitation显示文摘To the Editor:Hemorrhagic shock is a critical pathological process characterized by microcirculation dysfunction and hypoperfusion,with severe consequences of cell damage and organ dysfunction.Intestinal barrier dysfunction is a critical link of distant organ injury caused by hemorrhagic shock.Prophylactic treatment with stellate ganglion block(SGB)significantly reduces hemorrhagic shock-induced intestinal barrier damage. | Zhonghua Li Wendi Wang Qi Sun Tingjiao Suo Jiayi Zhai Huibo Du Zhen’ao Zhao Fulong Li Chunyu Niu Zigang Zhao | 2022 | Chinese Medical Journal2022，，8： | 2 |
| 9 | Characteristie of Cd Sorption in the Copper Tailings Wasteland Soil by Amended Dissolved Organic Matter from Fresh Manure and Manure Compost显示文摘 | Zigang LI Chuanzhou BIAN Xiaolei JIE | 2007 | African Journal of Bioteehnology2007，6，3： | 1 |
| 10 | Architecture design of GPS software receiver and implementation of its acquisition algorithm with fine frequency estimation 显示文摘 | Zhu Xuefen Chen Xiyuan Li Zigang | 2008 | Journal of Southeast University2008，24，1： | 1 |
| 11 | EBV encoded miR-BHRF1-1 potentiates viral lytic replication by downregulating host p53 in nasopharyngeal carcinoma显示文摘 | Zijian Li Xue Chen Lili Li Sufang Liu Lifang Yang Xiaoqian Ma Min Tang Ann M. Bode Zigang Dong Lunquan Sun Ya Cao | 2011 | International Journal of Biochemistry and Cell Biology2011，，2： | 1 |
| 12 | Characteristic of Cd sorption in the copper tailings wasteland soil by amended dissolved organic matter from fresh manure and manure compost显示文摘 | LI Zigang BIAN Chuanzhou JIE Xiaolei | | 0，，03： | 1 |
| 13 | Erianin suppresses constitutive activation of MAPK signaling pathway by inhibition of CRAF and MEK1/2显示文摘Constitutive activation of RAS-RAF-MEK-ERK signaling pathway(MAPK pathway)frequently occurs in many cancers harboring RAS or RAF oncogenic mutations.Because of the paradoxical activation induced by a single use of BRAF or MEK inhibitors,dual-target RAF and MEK treatment is thought to be a promising strategy.In this work,we evaluated erianin is a novel inhibitor of CRAF and MEK1/2 kinases,thus suppressing constitutive activation of the MAPK signaling pathway induced by BRAF V600E or RAS mutations.KinaseProfiler enzyme profiling,surface plasmon resonance(SPR),isothermal titration calorimetry(ITC),cellular thermal shift assay,computational docking,and molecular dynamics simulations were utilized to screen and identify erianin binding to CRAF and MEK1/2.Kinase assay,luminescent ADP detection assay,and enzyme kinetics assay were investigated to identify the efficiency of erianin in CRAF and MEK1/2 kinase activity.Notably,erianin suppressed BRAF V600E or RAS mutant melanoma and colorectal cancer cell by inhibiting MEK1/2 and CRAF but not BRAF kinase activity.Moreover,erianin attenuated melanoma and colorectal cancer in vivo.Overall,we provide a promising leading compound for BRAF V600E or RAS mutant melanoma and colorectal cancer through dual targeting of CRAF and MEK1/2. | Penglei Wang Xuechao Jia Bingbing Lu Han Huang Jialin Liu Xuejiao Liu Qiong Wu Yamei Hu Pan Li Huifang Wei Tingting Liu Dengyun Zhao Lingwei Zhang Xueli Tian Yanan Jiang Yan Qiao Wenna Nie Xinli Ma Ruihua Bai Cong Peng Zigang Dong Kangdong Liu | 2023 | Signal Transduction and Targeted Therapy2023，8，4： | 1 |
| 14 | Computer aided detection and diagnosis of colon polyps with morphological and texture features 显示文摘 | Wang Zigang Li Lihong Anderson J | 2004 | SPIE Medical Imging2004，5370，： | 1 |
| 15 | Tenure-based residential segregation in post-reform Chinese cities: a case study of Shanghai显示文摘 | Li Zigang Wu Fulong | 2008 | Trans- actions of the Institute of British Geographers2008，33，3： | 1 |
| 16 | Novel dual inhibitor for targeting PIM1 and FGFR1 kinases inhibits colorectal cancer growth in vitro and patient-derived xenografts in vivo显示文摘Colorectal cancer(CRC) is the second most common cause of cancer-related death in the world. The pro-viral integration site for Moloney murine leukemia virus 1(PIM1) is a proto-oncogene and belongs to the serine/threonine kinase family, which are involved in cell proliferation, migration,and apoptosis. Fibroblast growth factor receptor 1(FGFR1) is a tyrosine kinase that has been implicated in cell proliferation, differentiation and migration. Small molecule HCI-48 is a derivative of chalcone, a class of compounds known to possess anti-tumor, anti-inflammatory and antibacterial effects. However,the underlying mechanism of chalcones against colorectal cancer remains unclear. This study reports that HCI-48 mainly targets PIM1 and FGFR1 kinases, thereby eliciting antitumor effects on colorectal cancer growth in vitro and in vivo. HCI-48 inhibited the activity of both PIM1 and FGFR1 kinases in an ATPdependent manner, as revealed by computational docking models. Cell-based assays showed that HCI-48inhibited cell proliferation in CRC cells(HCT-15, DLD1, HCT-116 and SW620), and induced cell cycle arrest in the G2/M phase through modulation of cyclin A2. HCI-48 also induced cellular apoptosis, as evidenced by an increase in the expression of apoptosis biomarkers such as cleaved PARP, cleaved caspase 3 and cleaved caspase 7. Moreover, HCI-48 attenuated the activation of downstream components of the PIM1 and FGFR1 signaling pathways. Using patient-derived xenograft(PDX) murine tumor models,we found that treatment with HCI-48 diminished the PDX tumor growth of implanted CRC tissue expressing high protein levels of PIM1 and FGFR1. This study suggests that the inhibitory effect of HCI-48 on colorectal tumor growth is mainly mediated through the dual-targeting of PIM1 and FGFR1kinases. This work provides a theoretical basis for the future application of HCI-48 in the treatment of clinical CRC. | Fanxiang Yin Ran Zhao Dhilli Rao Gorja Xiaorong Fu Ning Lu Hai Huang Beibei Xu Hanyong Chen Jung-Hyun Shim Kangdong Liu Zhi Li Kyle Vaughn Laster Zigang Dong Mee-Hyun Lee | 2022 | Acta Pharmaceutica Sinica B2022，12，11： | 1 |
| 17 | Nanosecond pulsed electric fields prime mesenchymal stem cells to peptide ghrelin and enhance chondrogenesis and osteochondral defect repair in vivo显示文摘Mesenchymal stem cells(MSCs) are important cell sources in cartilage tissue development and homeostasis,and multiple strategies have been developed to improve MSCs chondrogenic differentiation with an aim of promoting cartilage regeneration.Here we report the effects of combining nanosecond pulsed electric fields(ns PEFs) followed by treatment with ghrelin(a hormone that stimulates release of growth hormone) to regulate chondrogenesis of MSCs.ns PEFs and ghrelin were observed to separately enhance the chondrogenesis of MSCs,and the effects were significantly enhanced when the bioelectric stimulation and hormone were combined,which in turn improved osteochondral tissue repair of these cells within Sprague Dawley rats.We further found that ns PEFs can prime MSCs to be more receptive to subsequent stimuli of differentiation by upregulated Oct4/Nanog and activated JNK signaling pathway.Ghrelin initiated chondrogenic differentiation by activation of ERK1/2 signaling pathway,and RNA-seq results indicated 243 genes were regulated,and JAK-STAT signaling pathway was involved.Interestingly,the sequential order of applying these two stimuli is critical,with ns PEFs pretreatment followed by ghrelin enhanced chondrogenesis of MSCs in vitro and subsequent cartilage regeneration in vivo,but not vice versa.This synergistic prochondrogenic effects provide us new insights and strategies for future cell-based therapies. | Kejia Li Litong Fan Jianjing Lin Boon Chin Heng Zhantao Deng Qiujian Zheng Jue Zhang Yangzi Jiang Zigang Ge | 2022 | Science China(Life Sciences)2022，65，5： | 1 |
| 18 | Facial paralysis in cerebral infarction:A case of misdiagnosis and literature review显示文摘Facial paralysis can be classified as central or peripheral facial paralysis based on the location of the underlying lesion,both of which demonstrate facial motor dysfunction.In the currently report,a patient admitted to the department of otology,First People’s Hospital of Qinhuangdao,presented with facial asymmetry as the initial symptom of a cerebral infarction and was first misdiagnosed as peripheral facial paralysis.The case is reported as follows. | Xin Li Xiaoyan Zhang Xiaobin Tian Zigang Jiang Baohuan Li | 2014 | Journal of Otology2014，9，4： | 1 |
| 19 | Enzymatic synthesis of phytosterol esters catalyzed by Candida rugosa lipase in water - in - PF6 mieroemulsion 显示文摘 | ZENG Chaoxi QI Suijian LI Zigang | 2015 | Bioproc Biosys Eng2015，38，： | 1 |
| 20 | A simple magnetic force-based cell patterning method using soft lithography显示文摘Dear Editor,Cell patterning is gaining more and more attention regarding a wide range of applications including cell biology,tissue engineering,and biosensor technology,to name a few.Magnetic force provides a promising tool to pattern cells because it is of excellent biocompatibility,tunable and re- | LI ShanShan LIU XiangQi CHAU Alicia PENG XiaoLing GUIDO Isabella WANG LiLi GE ZiGang XIONG ChunYang | 2015 | Science China(Life Sciences)2015，58，4： | 1 |
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