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| 1 | Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells显示文摘Spermatogonial 干细胞(SSC ) 能在移植以后生产众多的男配偶子进接受者睾丸,介绍为基因治疗和修改基因的动物的连续生产的一条珍贵途径。然而, SSC 的成功的基因操作被限制了,部分由于复杂性和当前可得到的基因编辑技术的低效率。这里,我们证明有效基因修正能用 CRISPR-Cas9 系统被介绍进 SSC。我们使用了 CRISPR-Cas9 系统变异在 SCC 的 EGFP transgene 或内长的 Crygc 基因。变异的 SSC 在移植以后经历了精子发生进不肥沃的老鼠睾丸的生精的小管。圆 spermatids 被产生并且在进成熟卵母细胞的注射以后,支持了显示相应变异的显型的异质接合的后代的生产。而且,在 Crygc 的一个引起疾病的变化(在 SSC 先存在的 Crygc −/−) 能乐意地到加入的 CRISPR-Cas9-induced nonhomologous 结束(NHEJ ) 或指导相同的修理(HDR ) 被修理,导致没有是的离开目标修正的证据带改正的基因的 SSC 线由整个染色体的定序出现。用从这些线产生的圆 spermatids 的授精在 100% 的效率与改正的显型产生了后代。我们的结果表明有效基因由 CRISPR-Cas9 系统在老鼠 SSC 编辑,并且提供在 SSC 经由基因修正治好基因疾病的原则的证明。 | Yuxuan Wu Hai Zhou Xiaoying Fan Ying Zhang Man Zhang Yinghua Wang Zhenfei Xie Meizhu Bai Qi Yin Dan Liang Wei Tang Jiaoyang Liao Chikai Zhou Wujuan Liu Ping Zhu Hongshan Guo Hong Pan Chunlian Wu Huijuan Shi Ligang Wu Fuchou Tang Jinsong Li | 2015 | Cell Research2015，25，1： | 37 |
| 2 | Parthenogenetic haploid embryonic stem cells efficiently support mouse generation by oocyte injection显示文摘 | YuxuanWu Li Yang Jinsong Li Cuiqing Zhong Zhenfei Xie Qi Yin Rui Dong Suming Yang | 2016 | Cell Research2016，26，1： | 13 |
| 3 | Dosage effect of multiple genes accounts for multisystem disorder of myotonic dystrophy type 1显示文摘Multisystem manifestations in myotonic dystrophy type 1(DM1)may be due to dosage reduction in multiple genes induced by aberrant expansion of CTG repeats in DMPK,including DMPK,its neighboring genes(SIX5 or DMWD)and downstream MBNLh However,direct evidence is lacking.Here,we develop a new strategy to generate mice carrying multigene heterozygous mutations to mimic dosage reduction in one step by injection of haploid embryonic stem cells with mutant Dmpk,Six5 and Mbnll into oocytes.The triple heterozygous mutant mice exhibit adult-onset DM1 phenotypes.With the additional mutation in Dmwd,the quadruple heterozygous mutant mice recapitulate many major manifestations in congenital DM1.Moreover,muscle stem cells in both models display reduced sternness,providing a unique model for screening small molecules for treatment of DM1.Our results suggest that the complex symptoms of DM1 result from the reduced dosage of multiple genes. | Qi Yin Hongye Wang Na Li Yifu Ding Zhenfei Xie Lifang Jin Yan Li Qiong Wang Xinyi Liu Liuqing Xu Qing Li Yongjian Ma Yanbo Cheng Kai Wang Cuiqing Zhong Qian Yu Wei Tang Wanjin Chen Wenjun Yang Fan Zhang Chen Ding Lan Bao Bin Zhou Ping Hu Jinsong Li | 2020 | Cell Research2020，30，2： | 7 |
| 4 | Influences of uncertainties to the generation feasible region for medium- and long-term electricity transaction显示文摘For the implementation of power market in China,medium-and Iong-term security checks are essential for bilateral transactions,of which the electricity quantity that constitutes the generation feasible region(GFR)is the target.However,uncertainties from load forecasting errors and transmission contingencies are threats to medium-and Iong-term electricity tradi ng in terms of their in flue nces on the GFR.In this paper,we prese nt a graphic distortio n pattern in a typical threegenerator system using the Monte Carlo method and projection theory based on security constrained economic dispatch.The underlying potential risk to GFR from uncertainties is clearly visualized,and their impact characteristics are discussed.A case study on detailed GFR distortion was included to dem on strate the effectiveness of this visualization model.The result implies that a small uncertainty could distort the GFR to a remarkable extent and that different line-contingency precipitates disparate the GFR distortion patterns,thereby eliciting great emphasis on load forecasting and line reliability in electricity transacti ons. | Yuyun Yang Zhenfei Tan Zhilin Jiang Jun Yao Xingqiang Wang Mingyuan Wang Yan Xie Zhiyun Hu | 2020 | Global Energy Interconnection2020，3，6： | 0 |
| 5 | Implications of COVID-19 for the Electricity Industry: A Comprehensive Review显示文摘As COVID-19 sweeps through the whole world,human activities have been changed significantly.Under such circumstances,the electricity sector is deeply affected and faced with great challenges.This paper provides a comprehensive review of the impacts that the pandemic has caused on the electricity sector.Electricity demand has dropped sharply as governments around the world executed lockdown restrictions,while the load composition and daily load profile have also changed.The share of renewable generation has increased against the decline of the total electricity generation.Changed power balance situation and increased uncertainty of demand have posed higher pressure on system operators,along with voltage violation issue and challenges for system maintenance and management.The electricity market is also substantially influenced,while longterm investment in clean energy is expected to be stable.The externality such as emission reduction is also discussed. | Haiwang Zhong Zhenfei Tan Yiliu He Le Xie Chongqing Kang | 2020 | CSEE Journal of Power and Energy Systems2020，6，3： | 0 |
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