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| 1 | Autophagy in bone homeostasis and the onset of osteoporosis显示文摘Autophagy is an evolutionarily conserved intracellular process,in which domestic cellular components are selectively digested for the recycling of nutrients and energy.This process is indispensable for cell homeostasis maintenance and stress responses.Both genetic and functional studies have demonstrated that multiple proteins involved in autophagic activities are critical to the survival,differentiation,and functioning of bone cells,including osteoblasts,osteocytes,and osteoclasts.Dysregulation at the level of autophagic activity consequently disturbs the balance between bone formation and bone resorption and mediates the onset and progression of multiple bone diseases,including osteoporosis.This review aims to introduce the topic of autophagy,summarize the understanding of its relevance in bone physiology,and discuss its role in the onset of osteoporosis and therapeutic potential. | Xing Yin Chenchen Zhou Jingtao Li Renkai Liu Bing Shi Quan Yuan Shujuan Zou | 2019 | Bone Research2019，7，4： | 25 |
| 2 | Blown sand motion within the sand-control system in the southern section of the Taklimakan Desert Highway显示文摘Although scientists have performed many studies in the Taklimakan Desert, few of them have reported the blown sand motion along the southern edge of the Taklimakan Desert Highway, which differs significantly from the northern region in terms of aeolian sand geomorphology and formation environment. Based on the field observation data of airflow and aeolian sand transport, continuous monitoring data of erosional and depositional processes between 14 April 2009 and 9 April 2011 and data of surface sand grains from the classical section along the southern edge of the Taklimakan Desert Highway, this paper reported the blown sand motion within the sand-control system of the highway. The main results are as follows: 1) The existing sand-control system is highly effective in preventing and controlling desertification. Wind velocities within the sand-control system were approximately 33%–100% of those for the same height above the mobile sand surface. Aeolian sand fluxes were approximately 0–31.21% of those of the mobile sand surface. Sand grains inside the system, with a mean diameter of 2.89 φ, were finer than those(2.15 φ) outside the system. In addition, wind velocities basically followed a logarithmic law, but the airflow along the classical section was mainly determined by topography and vegetation. 2) There were obvious erosional and depositional phenomena above the surface within the sand-control system, and these phenomena have very consistent patterns for all observation points in the two observed years. The total thicknesses of erosion and deposition ranged from 0.30 to 14.60 cm, with a mean value of 3.67 cm. In contrast, the deposition thicknesses were 1.90–22.10 cm, with a mean value of 7.59 cm, and the erosion thicknesses were 3.51–15.10 cm, with a mean value of 8.75 cm. The results will aid our understanding of blown sand within the sand-control system and provide a strong foundation for optimizing the sand-control system. | CHENG Hong HE Jiajia XU Xingri ZOU Xueyong WU Yongqiu LIU Chenchen DONG Yifan PAN Meihui WANG Yanzai ZHANG Hongyan | 2015 | Journal of Arid Land2015，7，5： | 8 |
| 3 | DNA N^6-methyladenine demethylase ALKBH1 enhances osteogenic differentiation of human MSCs显示文摘ALKBH1 was recently discovered as a demethylase for DNA N^6-methyladenine(N6-m A), a new epigenetic modification, and interacts with the core transcriptional pluripotency network of embryonic stem cells.However, the role of ALKBH1 and DNA N6-m A in regulating osteogenic differentiation is largely unknown.In this study, we demonstrated that the expression of ALKBH1 in human mesenchymal stem cells(MSCs)was upregulated during osteogenic induction. Knockdown of ALKBH1 increased the genomic DNA N6-m A levels and significantly reduced the expression of osteogenic-related genes, alkaline phosphatase activity, and mineralization. ALKBH1-depleted MSCs also exhibited a restricted capacity for bone formation in vivo.By contrast, the ectopic overexpression of ALKBH1 enhanced osteoblastic differentiation. Mechanically,we found that the depletion of ALKBH1 resulted in the accumulation of N6-m A on the promoter region of ATF4, which subsequently silenced ATF4 transcription. In addition, restoring the expression of ATP by adenovirus-mediated transduction successfully rescued osteogenic differentiation. Taken together, our results demonstrate that ALKBH1 is indispensable for the osteogenic differentiation of MSCs and indicate that DNA N6-m A modifications area new mechanism for the epigenetic regulation of stem cell differentiation. | Chenchen Zhou Yuting Liu Xiaobing Li Jing Zou Shujuan Zou | 2016 | Bone Research2016，4，3： | 6 |
| 4 | The RNA-binding protein Musashi2 governs osteoblast-adipocyte lineage commitment by suppressing PPARγsignaling显示文摘Osteoporosis caused by aging is characterized by reduced bone mass and accumulated adipocytes in the bone marrow cavity. How the balance between osteoblastogenesis and adipogenesis from bone marrow mesenchymal stem cells(BMSCs) is lost upon aging is still unclear. Here, we found that the RNA-binding protein Musashi2(Msi2) regulates BMSC lineage commitment. Msi2 is commonly enriched in stem cells and tumor cells. We found that its expression was downregulated during adipogenic differentiation and upregulated during osteogenic differentiation of BMSCs. Msi2 knockout mice exhibited decreased bone mass with substantial accumulation of marrow adipocytes, similar to aging-induced osteoporosis. Depletion of Msi2 in BMSCs led to increased adipocyte commitment. Transcriptional profiling analysis revealed that Msi2 deficiency led to increased PPARγ signaling.RNA-interacting protein immunoprecipitation assays demonstrated that Msi2 could inhibit the translation of the key adipogenic factor Cebpα, thereby inhibiting PPAR signaling. Furthermore, the expression of Msi2 decreased significantly during the aging process of mice, indicating that decreased Msi2 function during aging contributes to abnormal accumulation of adipocytes in bone marrow and osteoporosis. Thus, our results provide a putative biochemical mechanism for aging-related osteoporosis, suggesting that modulating Msi2 function may benefit the treatment of bone aging. | Jinlong Suo Sihai Zou Jinghui Wang Yujiao Han Lingli Zhang Chenchen Lv Bo Jiang Qian Ren Long Chen Lele Yang Ping Ji Xianyou Zheng Ping Hu Weiguo Zou | 2022 | Bone Research2022，10，3： | 5 |
| 5 | Runxl protects against the pathological progression of osteoarthritis显示文摘Runt-related transcription factor-1(Runxl)is required for chondrocyte-to-osteoblast lineage commitment by enhancing both chondrogenesis and osteogenesis during vertebrate development.However,the potential role of Runxl in joint diseases is not well known.In the current study,we aimed to explore the role of Runxl in osteoarthritis induced by anterior cruciate ligament transaction(ACLT)surgery.We showed that chondrocyte-specific Runxl knockout(Runx1f/fCol2a1-Cre)aggravated cartilage destruction by accelerating the loss of proteoglycan and collagen II in early osteoarthritis.Moreover,we observed thinning and ossification of the growth plate,a decrease in chondrocyte proliferative capacity and the loss of bone matrix around the growth plate in late osteoarthritis.We overexpressed Runxl by adeno-associated virus(AAV)in articular cartilage and identified its protective effect by slowing the destruction of osteoarthritis in cartilage in early osteoarthritis and alleviating the pathological progression of growth plate cartilage in late osteoarthritis.ChIP-seq analysis identified new targets that interacted with Runxl in cartilage pathology,and we confirmed the direct interactions of these factors with Runxl by ChIP-qPCR.This study helps us to understand the function of Runxl in osteoarthritis and provides new clues for targeted osteoarthritis therapy. | Chenchen Zhou Yujia Cui Yueyi Yang Daimo Guo Demao Zhang Yi Fan Xiaobing Li Jing Zou Jing Xie | 2021 | Bone Research2021，9，4： | 4 |
| 6 | Parathyroid hormone increases alveolar bone homoeostasis during orthodontic tooth movement in rats with periodontitis via crosstalk between STAT3 and β-catenin显示文摘Periodontitis patients are at risk of alveolar bone loss during orthodontic treatment.The aim of this study was to investigate whether intermittent parathyroid hormone(1–34)treatment(iPTH)could reduce alveolar bone loss during orthodontic tooth movement(OTM)in individuals with periodontitis and the underlying mechanism.A rat model of OTM in the context of periodontitis was established and alveolar bone loss was observed.The control,iPTH and iPTH+stattic groups received injections of vehicle,PTH and vehicle,or PTH and the signal transducer and activator of transcription 3(STAT3)inhibitor stattic,respectively.iPTH prevented alveolar bone loss by enhancing osteogenesis and suppressing bone resorption in the alveolar bone during OTM in rats with periodontitis.This effect of iPTH was along with STAT3 activation and reduced by a local injection of stattic.iPTH promoted osteoblastic differentiation and might further regulate the Wnt/β-catenin pathway in a STAT3-dependent manner.The findings of this study suggest that iPTH might reduce alveolar bone loss during OTM in rats with periodontitis through STAT3/β-catenin crosstalk. | Cheng Zhang Tiancheng Li Chenchen Zhou Li Huang Yuyu Li Han Wang Peipei Duan Shujuan Zou Li Mei | 2020 | International Journal of Oral Science2020，12，4： | 4 |
| 7 | Erratum to:Influence of the time-step on the production of free nucleons and pions from heavy-ion collisions around 1 GeV/nucleon [Sci.China-Phys.Mech.Astron.59,122011(2016)]显示文摘In the References of the original publication[1]of this paper,the authors of ref.[26]should be'S.A.Bass,et al.(UrQMD Collaboration)',the authors of ref.[27]should be'M.Ble-icher,et al.(UrQMD Collaboration)'.The authors of ref.[35]should be'W.Reisdorf,et al.(FOPI Collaboration)', | LiYan Zou Miao Li ChenChen Guo YongJia Wang QingFeng Li Ling Liu | 2017 | Science China(Physics,Mechanics & Astronomy)2017，60，2： | 1 |
| 8 | Alkaline post-treat- ment for improved sludge anaerobic digestion显示文摘 | LI Huan ZOU Shuxin L1 Chenchen | 2013 | Bioresource Technology2013，140，： | 1 |
| 9 | Cyclic di-adenosine monophosphate regulates the osteogenic and adipogenic differentiation of hPDLSCs via MAPK and NF-κB signaling显示文摘Cyclic di-adenosine monophosphate(c-di-AMP)is a bacterial second messenger that can be recognized by infected host cells and activate the immunoinflammatory response.The purpose of this study is to demonstrate the effect of c-di-AMP on the differentiation of human periodontal ligament stem cells(hPDLSCs)and its underlying mechanisms.In the present study,we find that the gingival crevicular fluid(GCF)of patients with chronic periodontitis has a higher expression level of c-di-AMP than that of healthy people.In vitro,c-di-AMP influences the differentiation of hPDLSCs by upregulating Toll-like receptors(TLRs);specifically,it inhibits osteogenic differentiation by activating NF-κB and ERK/MAPK and promotes adipogenic differentiation through the NF-κB and p38/MAPK signaling pathways.Inhibitors of TLRs or activated pathways reduce the changes induced by c-di-AMP.Our results establish the potential correlation among bacterial c-di-AMP,periodontal tissue homeostasis and chronic periodontitis pathogenesis. | Sirui Chen Zuping Wu Yuying He Li Zhu Jiahe Wang Hengyi Lin Jing Xie Chenchen Zhou Shujuan Zou | 2023 | Acta Biochimica et Biophysica Sinica2023，55，3： | 1 |
| 10 | Influence of the time-step on the production of free nucleons and pions from heavy-ion collisions around 1 GeV/nucleon显示文摘By considering different values of the time-step for the potential updates in the ultra-relativistic quantum molecular dynamics(UrQMD) model, we examine its influence on observables, such as the yield and collective flow of nucleons and pions from heavyion collisions around 1 GeV/nucleon. It is found that these observables are affected to some extent by the choice of the time-step,and the impact of the time-step on the pion-related observables is more visible than that on the nucleon-related ones. However,its effect on the π-/π+yield ratio and elliptic flow difference between neutrons and protons, which have been taken as sensitive observables for probing the density-dependent nuclear symmetry energy at high densities, is fairly weak. | LiYan Zou Miao Li ChenChen Guo YongJia Wang QingFeng Li Ling Liu | 2016 | Science China(Physics,Mechanics & Astronomy)2016，59，12： | 1 |
| 11 | Hydrogel platform with tunable stiffness based on magnetic nanoparticles cross-linked GelMA for cartilage regeneration and its intrinsic biomechanism显示文摘Cartilage injury affects numerous individuals,but the efficient repair of damaged cartilage is still a problem in clinic.Hydrogel is a potent scaffold candidate for tissue regeneration,but it remains a big challenge to improve its mechanical property and figure out the interaction of chondrocytes and stiffness.Herein,a novel hybrid hydrogel with tunable stiffness was fabricated based on methacrylated gelatin(GelMA)and iron oxide nanoparticles(Fe_(2)O_(3))through chemical bonding.The stiffness of Fe_(2)O_(3)/GelMA hybrid hydrogel was controlled by adjusting the concentration of magnetic nanoparticles.The hydrogel platform with tunable stiffness modulated its cellular properties including cell morphology,microfilaments and Young’s modulus of chondrocytes.Interestingly,Fe_(2)O_(3)/GelMA hybrid hydrogel promoted oxidative phosphorylation of mitochondria and facilitated catabolism of lipids in chondrocytes.As a result,more ATP and metabolic materials generated for cellular physiological activities and organelle component replacements in hybrid hydrogel group compared to pure GelMA hydrogel.Furthermore,implantation of Fe_(2)O_(3)/GelMA hybrid hydrogel in the cartilage defect rat model verified its remodeling potential.This study provides a deep understanding of the bio-mechanism of Fe_(2)O_(3)/GelMA hybrid hydrogel interaction with chondrocytes and indicates the hydrogel platform for further application in tissue engineering. | Chenchen Zhou Chunli Wang Kang Xu Zhixing Niu Shujuan Zou Demao Zhang Zhiyong Qian Jinfeng Liao Jing Xie | 2023 | Bioactive Materials2023，，7： | 1 |
| 12 | circ_0003204 regulates the osteogenic differentiation of human adipose-derived stem cells via miR-370-3p/HDAC4 axis显示文摘Human adipose-derived stem cells(hASCs)are a promising cell type for bone tissue regeneration.Circular RNAs(circRNAs)have been shown to play a critical role in regulating various cell differentiation and involve in mesenchymal stem cell osteogenesis.However,how circRNAs regulate hASCs in osteogenesis is still unclear.Herein,we found circ_0003204 was significantly downregulated during osteogenic differentiation of hASCs.Knockdown of circ_0003204 by si RNA or overexpression by lentivirus confirmed circ_0003204 could negatively regulate the osteogenic differentiation of hASCs.We performed dual-luciferase reporting assay and rescue experiments to verify circ_0003204 regulated osteogenic differentiation via sponging miR-370-3p.We predicted and confirmed that miR-370-3p had targets in the 3′-UTR of HDAC4 m RNA.The following rescue experiments indicated that circ_0003204 regulated the osteogenic differentiation of hASCs via miR-370-3p/HDAC4 axis.Subsequent in vivo experiments showed the silencing of circ_0003204 increased the bone formation and promoted the expression of osteogenic-related proteins in a mouse bone defect model,while overexpression of circ_0003204 inhibited bone defect repair.Our findings indicated that circ_0003204 might be a promising target to promote the efficacy of hASCs in repairing bone defects. | Liyuan Yu Kai Xia Jing Zhou Zhiai Hu Xing Yin Chenchen Zhou Shujuan Zou Jun Liu | 2022 | International Journal of Oral Science2022，14，3： | 1 |
| 13 | LITTIP/Lgr6/HnRNPK complex regulates cementogenesis via Wnt signaling显示文摘Orthodontically induced tooth root resorption(OIRR)is a serious complication during orthodontic treatment.Stimulating cementum repair is the fundamental approach for the treatment of OIRR.Parathyroid hormone(PTH)might be a potential therapeutic agent for OIRR,but its effects still lack direct evidence,and the underlying mechanisms remain unclear.This study aims to explore the potential involvement of long noncoding RNAs(lncRNAs)in mediating the anabolic effects of intermittent PTH and contributing to cementum repair,as identifying lncRNA-disease associations can provide valuable insights for disease diagnosis and treatment.Here,we showed that intermittent PTH regulates cell proliferation and mineralization in immortalized murine cementoblast OCCM-30 via the regulation of the Wnt pathway.In vivo,daily administration of PTH is sufficient to accelerate root regeneration by locally inhibiting Wnt/β-catenin signaling.Through RNA microarray analysis,lncRNA LITTIP(LGR6 intergenic transcript under intermittent PTH)is identified as a key regulator of cementogenesis under intermittent PTH.Chromatin isolation by RNA purification(ChIRP)and RNA immunoprecipitation(RIP)assays revealed that LITTIP binds to mRNA of leucine-rich repeatcontaining G-protein coupled receptor 6(LGR6)and heterogeneous nuclear ribonucleoprotein K(HnRNPK)protein.Further cotransfection experiments confirmed that LITTIP plays a structural role in the formation of the LITTIP/Lgr6/HnRNPK complex.Moreover,LITTIP is able to promote the expression of LGR6 via the RNA-binding protein HnRNPK.Collectively,our results indicate that the intermittent PTH administration accelerates root regeneration via inhibiting Wnt pathway.The lncRNA LITTIP is identified to negatively regulate cementogenesis,which activates Wnt/β-catenin signaling via high expression of LGR6 promoted by HnRNPK. | Tiancheng Li Han Wang Yukun Jiang Shuo Chen Danyuan Huang Zuping Wu Xing Yin Chenchen Zhou Yuyu Li Shujuan Zou | 2023 | International Journal of Oral Science2023，15，3： | 0 |
| 14 | Continuously Quantifying Oral Chemicals Based on Flexible Hybrid Electronics for Clinical Diagnosis and Pathogenetic Study显示文摘Simultaneous monitoring of diverse salivary parameters can reveal underlying mechanisms of intraoral biological processes and offer profound insights into the evolution of oral diseases.However,conventional analytical devices with bulky volumes,rigid formats,and discrete sensing mechanisms deviate from the requirements of continuous biophysiological quantification,resulting in huge difficulty in precise clinical diagnosis and pathogenetic study.Here,we present a flexible hybrid electronic system integrated with functional nanomaterials to continuously sense Ca^(2+),pH,and temperature for wireless real-time oral health monitoring.The miniaturized system with an island-bridge structure that is designed specifically to fit the teeth is only 0.4g in weight and 31.5×8.5×1.35 mm^(3) in dimension,allowing effective integration with customized dental braces and comfort attachment on teeth.Characterization results indicate high sensitivities of 30.3 and 60.6 mV/decade for Ca^(2+)and pH with low potential drifts.The system has been applied in clinical studies to conduct Ca^(2+)and pH mappings on carious teeth,biophysiological monitoring for up to 12 h,and outcome evaluation of dental restoration,providing quantitative data to assist in the diagnosis and understanding of oral diseases.Notably,caries risk assessment of 1o human subjects using the flexible system validates the important role of saliva buffering capacity in caries pathogenesis.The proposed flexible system may offer an open platform to carry diverse components to support both clinical diagnosis and treatment as well as fundamental researchfororaldiseases and induced systemicdiseases. | Wei Lingo Yinghui Wang ingyu Lu Xue Shang Ziyue Wu Zhaorun Chen Xueting Li Chenchen Zou Jinjie Yan Yunjie Zhou Jie Liu Hongjie Li Kehua Que Xian Huan | 2022 | Research2022，，4： | 0 |
| 15 | Ovarian Cancer Screening Based on Mixture Change-Point Model显示文摘Ovarian cancer is one of the most deadly female genital malignant tumors in many regions while an effective early screening strategy can save numerous lives.CA125 and HE4 are tumor markers validated efficacious as well as most commonly used in recent screening research of ovarian cancer.In this paper,the authors construct a change-point and mixture model on the basis of longitudinal CA125 and HE4 levels and estimated parameters using maximum likelihood method with the preclinical duration assumed right-censored,which is more adaptive and yields comparable results in comparison to the Bayesian approach raised by Skates.Consistency of estimators is proved.The authors also run a 5-year simulation of sequential screening by calculating the risk of cancer and hypothesis testing the true incidence time respectively.Results show that diagnosis based on hypothesis test performs better in early detection. | ZOU Chenchen FANG Xiangzhong ZHAI Guanghe | 2015 | Journal of Systems Science & Complexity2015，28，2： | 0 |
| 16 | Palladium-Catalyzed Intramolecular Dehydrogenative Arylboration of Alkenes显示文摘The palladium-catalyzed borylative cyclization via C—H activation has been developed.By this chemistry,the indole-fused dihydro-pyrrole motif,which is a kind of important unit in natural products and bio-active molecules,could be constructed and installed with a boric ester group.Furthermore,the utilities of products have been illustrated by the study of further transformations.Importantly,by using chiral ligand,the enantioselectivity of this borylative cyclization reaction could be controlled.Moreover,the borylative mechanism,which should proceed through a Pd(II)/Pd(IV)catalytic cycle,has been proposed based on the DFT calculations. | Chenchen Zou Hongli Wu Yuqi Ji Penglin Zhang Huanhuan Cui Genping Huang Chun Zhang | 2022 | Chinese Journal of Chemistry2022，40，20： | 0 |
| 17 | The pseudogene DUXAP10 contributes to gefitinib resistance in NSCLC by repressing OAS2 expression显示文摘Gefitinib,an epidermal growth factor receptor-tyrosine kinase inhibitor(EGFR-TKI),is the currently recommended first-line therapy for advanced EGFR-mutant lung cancer,and understanding the mechanism of resistance is the key to formulating therapeutic strategies for EGFR-TKIs.In this study,we evaluate the expression patterns and potential biological functions of the pseudogene DUXAP10 in gefitinib resistance.We find that pseudogene DUXAP10 expression is significantly upregulated in NSCLC gefitinib-resistant cells and tissues.Gain and loss of function assays reveal that knockdown of DUXAP10 by siRNA reverses gefitinib resistance both in vitro and in vivo.Furthermore,DUXAP10 interacts with the histone methyltransferase enhancer of zeste homolog 2(EZH2)to repress the expression of 2′,5′-oligoadenylate synthetase(OAS2).Overall,our study highlights the pivotal role of DUXAP10 in gefitinib resistance,and the DUXAP10/EZH2/OAS2 axis might be a promising therapeutic target to overcome acquired gefitinib resistance in NSCLC. | Shengnan Ren Ya Zhu Siying Wang Qinqiu Zhang Niu Zhang Xiaoteng Zou Chenchen Wei Zhaoxia Wang | 2023 | Acta Biochimica et Biophysica Sinica2023，55，1： | 0 |
| 18 | Preparation and Electrorheological Response of PAL/TiO_(2)/PANI Nanorods显示文摘Using palygorskite(PAL)as template,the PAL/TiO_(2)/PANI nano-rods were synthesized by heterogeneous precipitation and in-situ polymerization.The synthesized PAL/TiO_(2)/PANI nanorods were used as a novel electrorheological(ER)fluid by mixing with silicone oil,which showed excellent ER effect.The yield stress of the PAL/TiO_(2)/PANI based ER fluid(15 vol%)reached 8.8 kPa under 4 kV mm^(−1) electric field.The dynamic shear stress of the PAL/TiO_(2)/PANI based ER fluid could maintain a stable level in the shear rate range of 0.1–100 s^(−1).Furthermore,the PAL/TiO_(2)/PANI ER fluid exhibited excellent suspension stability. | Ling Wang Chenchen Huang Ping Zhang Fenghua Liu Ting Zou Zhixiang Li Jianfei Zhang Gaojie Xu | 2021 | Journal of Renewable Materials2021，9，10： | 0 |
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