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| 1 | Protective effects of human umbilical cord mesenchymal stem cell vein transplantation against spinal cord ischemia/reperfusion injury in rats显示文摘BACKGROUND: The majority of studies addressing spinal cord ischemia/reperfusion injury (SCIRI) have focused on drugs, proteins, cytokines, and various surgical techniques. A recent study reports that human umbilical cord mesenchymal stem cell (hUCMSC) transplantation achieves good therapeutic effects, but the mechanisms underlying nerve protection remain poorly understood. OBJECTIVE: To observe survival of transplanted hUCMSCs in SCIRI rat models and the influence on motor function in the hind limbs, to determine interleukin-8 expression and cellular apoptosis in spinal cord tissues, and to verify the hypothesis that hUCMSC transplantation exhibits protective effects on SCIRI. DESIGN, TIME AND SETTING: A randomized, controlled, animal experiment was performed at the Laboratory of the Department of Orthopedics in the First Affiliated Hospital of Soochow University, China between January 2007 and December 2008. MATERIALS: hUCMSCs were harvested from umbilical cord blood of healthy pregnant women after parturition in the Obstetrical Department of the First Affiliated Hospital of Soochow University, China. Rabbit anti-human BrdU monoclonal antibody was provided by DAKO, USA. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) Kit and enzyme-linked immunosorbent assay (ELISA) Kit were purchased by Wuhan Boster, China. METHODS: A total of 72 healthy, Wistar, adult rats were randomly assigned to three groups: sham-surgery, model, and transplantation, with 24 rats in each group. SCIRI was induced in the model and transplantation groups via the abdominal aorta block method. The infrarenal abdominal aorta was not blocked in the sham-surgery group. Prior to abdominal aorta occlusion, 0.2-0.3 mL bromodeoxyuridine (BrdU)-labeled hUCMSCs suspension (cell concentration 5 × 103/μL) was injected through the great saphenous vein of the hind limb, and an equal volume of physiological saline was administered to the model and sham-surgery groups. MAIN OUTCOME MEASURES: Pathological observation of rat spinal cord tissues was performed by hematoxylin-eosin staining at 6, 24, and 48 hours post-surgery. Immunohistochemistry was applied to determine hUCMSCs survival in the spinal cord. The amount of cellular apoptosis and interleukin-8 expression in spinal cord tissues was assayed utilizing the TUNEL and ELISA methods, respectively. Motor function in the hind limbs was evaluated according to Jacob's score. RESULTS: Numerous BrdU-positive cells were observed in spinal cord tissues from the transplantation group. The number of apoptotic cells and interleukin-8 levels significantly decreased in the transplantation group (P < 0.05), pathological injury was significantly ameliorated, and motor function scores significantly increased (P < 0.05) compared with the model group. CONCLUSION: Via vein transplantation, hUCMSCs were shown to reach and survive in the injury area. Results suggested that the transplanted hUCMSCs contributed to significantly improved pathological changes in the injured spinal cord, as well as motor function, following SCIRI. The protective mechanism correlated with inhibition of cellular apoptosis and reduced production of inflammatory mediators. | Jun Zou Minfeng Gan Xuesong Zhu Dechun Geng Huilin Yang | 2010 | Neural Regeneration Research2010，5，3： | 7 |
| 2 | Protective effects of sirtuin 3 on titanium particle-induced osteogenic inhibition by regulating the NLRP3 inflammasome via the GSK-3β/β-catenin signalling pathway显示文摘Periprosthetic osteolysis(PPO)remains the key factor in implant failure and subsequent revision surgery and is mainly triggered by wear particles.Previous studies have shown that inhibition of osteoblastic differentiation is the most widespread incident affecting the interface of trabecular and loosening prostheses.Additionally,the NLRP3 inflammasome is activated by prosthetic particles.Sirtuin3,an NAD+-dependent deacetylase of mitochondria,regulates the function of mitochondria in diverse activities.However,whether SIRT3 can mitigate wear debris-induced osteolysis by inhibiting the NLRP3 inflammasome and enhancing osteogenesis has not been previously reported.Therefore,we investigated the role of SIRT3 during the process of titanium(Ti)particle-induced osteolysis.We revealed that upregulated SIRT3 dramatically attenuated Ti particle-induced osteogenic inhibition through suppression of the NLRP3 inflammasome and improvement of osteogenesis in vivo and in vitro.Moreover,we found that SIRT3 interference in the process of Ti particle-induced osteolysis relied on the GSK-3β/β-catenin signalling pathway.Collectively,these findings indicated that SIRT3 may serve as a rational new treatment against debris-induced PPO by deacetylase-dependent inflammasome attenuation. | Kai Zheng Jiaxiang Bai Ning Li Meng Li Houyi Sun Weicheng Zhang Gaoran Ge Xiaolong Liang Huaqiang Tao Yi Xue Yuefeng Hao Chen Zhu Yaozeng Xu Dechun Geng | 2021 | Bioactive Materials2021，6，10： | 5 |
| 3 | Overexpression of OsSPL9 enhances accumulation of Cu in rice grain and improves its digestibility and metabolism显示文摘Copper(Cu)is an essential trace mineral element for all forms of life,and is an important structural component and co-factor for a variety of metalloenzymes(Pen^a et al.,1999;Bertinato and L'Abbe,2004).In humans,Cu deficiency is not common because of the ubiquitous occurrence of Cu and ease of gastrointestinal absorption(Zidar et al.,1977;Uauy et al.,1998).However,because of the low Cu content in most sources of feed,the use of Cu as a growth promoter to maximize animal production has been well documented in animal feeding(Braude,1945;Lu et al.,2010).Pre- | Mingfeng Tang Chuanshe Zhou Lu Meng Donghai Mao Can Peng Yuxing Zhu Daoyou Huang Zhiliang Tan Caiyan Chen Chengbing Liu Dechun Zhang | 2016 | Journal of Genetics and Genomics2016，43，11： | 2 |
| 4 | Targeting strategies for bone diseases:signaling pathways and clinical studies显示文摘Since the proposal of Paul Ehrlich’s magic bullet concept over 100 years ago,tremendous advances have occurred in targeted therapy.From the initial selective antibody,antitoxin to targeted drug delivery that emerged in the past decades,more precise therapeutic efficacy is realized in specific pathological sites of clinical diseases.As a highly pyknotic mineralized tissue with lessened blood flow,bone is characterized by a complex remodeling and homeostatic regulation mechanism,which makes drug therapy for skeletal diseases more challenging than other tissues.Bone-targeted therapy has been considered a promising therapeutic approach for handling such drawbacks.With the deepening understanding of bone biology,improvements in some established bone-targeted drugs and novel therapeutic targets for drugs and deliveries have emerged on the horizon.In this review,we provide a panoramic summary of recent advances in therapeutic strategies based on bone targeting.We highlight targeting strategies based on bone structure and remodeling biology.For bone-targeted therapeutic agents,in addition to improvements of the classic denosumab,romosozumab,and PTH1R ligands,potential regulation of the remodeling process targeting other key membrane expressions,cellular crosstalk,and gene expression,of all bone cells has been exploited.For bone-targeted drug delivery,different delivery strategies targeting bone matrix,bone marrow,and specific bone cells are summarized with a comparison between different targeting ligands.Ultimately,this review will summarize recent advances in the clinical translation of bone-targeted therapies and provide a perspective on the challenges for the application of bone-targeted therapy in the clinic and future trends in this area. | Hao Xu Wentao Wang Xin Liu Wei Huang Chen Zhu Yaozeng Xu Huilin Yang Jiaxiang Bai Dechun Geng | 2023 | Signal Transduction and Targeted Therapy2023，8，6： | 2 |
| 5 | Isobaric vapor-liquid equilibria for binary and ternary mixtures of propanal, propanol, and propanoic acid 显示文摘 | GAO Daming ZHU Dechun ZHANG Lingyun | 2010 | J Chem Eng Data2010，55，12： | 1 |
| 6 | Dissolved oxygen in aeration-driven piezo-catalytic for antibiotics pollutants removal in water显示文摘The misuse of antibiotics and oxygen-lacking in aquaculture causes serious water environmental problems.Herein,a piezoelectic odd-layered MoS_(2)is prepared and applied to piezo-catalytic remove tinidazole(TNZ)and other antibiotic pollutants with aeration as a piezo-driving force.About 89.6%of TNZ can be degraded by MoS_(2)under aeration in the presence of dissolved oxygen with a reaction rate constant of0.15 min^(-1),which is 2.4 times higher than that under N2atmosphere and quiescence conditions.Quenching experiments and electron paramagnetic resonance(EPR)tests identify that singlet oxygen(^(1)O_(2))and superoxide radical(O_(2)^(·-))are dominant reactive oxygen species in MoS_(2)/aeration system.These results demonstrate that MoS2can trigger a piezoelectric effect and produce charge carriers to generate reactive oxygen species with dissolved oxygen(DO)for contaminant degradation with the turbulence and water bubbles rupture driven by aeration. | Minxian Zhang Wanqian Guo Yingyin Chen Dechun He Abdulgalim B.Isaev Mingshan Zhu | 2023 | Chinese Chemical Letters2023，34，9： | 0 |
| 7 | Penile erectile dysfunction after brachial plexus root avulsion injury in rats显示文摘Our previous studies have demonstrated that some male patients suffering from brachial plexus injury,particularly brachial plexus root avulsion,show erectile dysfunction to varying degrees.However,the underlying mechanism remains poorly understood.In this study,we evaluated the erectile function after establishing brachial plexus root avulsion models with or without spinal cord injury in rats.After these models were established,we administered apomorphine(via a subcutaneous injection in the neck) to observe changes in erectile function.Rats subjected to simple brachial plexus root avulsion or those subjected to brachial plexus root avulsion combined with spinal cord injury had significantly fewer erections than those subjected to the sham operation.Expression of neuronal nitric oxide synthase did not change in brachial plexus root avulsion rats.However,neuronal nitric oxide synthase expression was significantly decreased in brachial plexus root avulsion + spinal cord injury rats.These findings suggest that a decrease in neuronal nitric oxide synthase expression in the penis may play a role in erectile dysfunction caused by the combination of brachial plexus root avulsion and spinal cord injury. | Guo Fu Bengang Qin Li Jiang Xijun Huang Qinsen Lu Dechun Zhang Xiaolin Liu Jiakai Zhu Jianwen Zheng Xuejia Li Liqiang Gu | 2014 | Neural Regeneration Research2014，9，20： | 0 |
| 8 | Study on The Identification System of Deteriorated Conditions for The Model of Cable-stayed Bridges Applying A Self-Organizing Feature Map显示文摘StudyonTheIdentificationSystemofDeterioratedConditionsforTheModelofCablestayedBridgesApplyingASelfOrganizingFeatureMapHuXio... | Hu XiongChen Zhaoneng,Tong Dechun,Zhu JimeiShanghai Maritime UniversityShanghai Jiaotong University1550 Podong Dadao,Shanghai 200135,1954 Huashan Rod,Shanghai 200030,P.R.ChinaP.R. China | 1998 | International Journal of Plant Engineering and Management1998，3，1： | 0 |
| 9 | A Full-Newton Step Feasible Interior-Point Algorithm for the Special Weighted Linear Complementarity Problems Based on a Kernel Function显示文摘In this paper,a new full-Newton step primal-dual interior-point algorithm for solving the special weighted linear complementarity problem is designed and analyzed.The algorithm employs a kernel function with a linear growth term to derive the search direction,and by introducing new technical results and selecting suitable parameters,we prove that the iteration bound of the algorithm is as good as best-known polynomial complexity of interior-point methods.Furthermore,numerical results illustrate the efficiency of the proposed method. | GENG Jie ZHANG Mingwang ZHU Dechun | 2024 | Wuhan University Journal of Natural Sciences2024，29，1： | 0 |
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