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| 1 | Expression of nuclear factor-KB in hepatocellular carcinoma and its relation with the X protein of hepatitis B virus显示文摘AIM In this study we investigated therelationship of the X protein of HBV and nuclearfactor-KB (NF-κB) and the expression of NF-KB inhuman hepatocellular carcinoma tissues.METHODS Immunohistochemistry SP methodwas used to detect the expression of NF-κB and the X protein of HBV in human hepatocellularcarcinoma tissues of 52 cases. Gene transfectionmediated by lipofectamine was used to transfectthe eukaryotic expression vector pCDNA3. 1-HBXof HBV x gene into human hepatocellularcarcinoma cell line HCC-9204 and NF-κB wasdetected.RESULTS NF-κB was widely expressed inhuman hepatocellular carcinoma tissues in atotal of 52 cases and its expression was relatedto the X protein of HBV. NF-KB was localizedboth in the cytoplasm and . The nuclei ofhepatocellular carcinoma cells in 11 cases whichwere positive for the X protein of HBV while in 41cases negative for the X protein of HBV, NF-Kbwas only localized in the cytoplasm ofhepatocellular carcinoma cells but translocatedto the nuclei of hepatocellular carcinoma cellsafter the eukaryotic expression vectorpCDNA3.1-HBX was transfected into HCC-9204cells.CONCLUSION This study strongly suggeststhat the nuclear factor NF-KB is widely expressedin hepatocellular carcinoma tissues in differentstyles according to the expression of the Xprotein of HBV. NF-κB is abnormally activated inhepatocellular carcinoma, which is probablyrelated to the X protein of HBV. The X protein ofHBV can activate NF-κB to translocate into nucleiof hepatocellular carcinoma cells. | Shuang Ping Guo~1 Wen Liang Wang~1 Yu Qiang Zhai~2 Yi Ling Zhao~1 ~1Department of Pathology,Xijing Hospital of the Fourth Military Medical University,Xi’an,China ~2Department of Urology,the Central Hospital of Xi’an,China | 2001 | World Journal of Gastroenterology2001，7，3： | 55 |
| 2 | Berberine protects rat heart from ischemia/ reperfusion injury via activating JAK2/STAT3 signaling and attenuating endoplasmic reticulum stress显示文摘目的: Berberine (BBR ) ,从根茎 coptidis 孤立的导出 isoquinoline 的碱,施加 cardioprotective 效果。因为 endoplasmic 蜂窝胃(嗯)应力在导致的心肌的 ischemia/reperfusion ( MI/R )起一个枢轴的作用 apoptosis ,检验 BBR 的保护的效果是否源于 modulating 有趣嗯在 MI/R 损害期间强调层次,并且在这 process.Methods 定义发信号的机制:雄的老鼠与 BBR 被对待( 200 mg· ; kg −1·d−1, ig )为 2 个星期,然后使外科遭到了到 MI/R 。心脏的尺寸和功能用 echocardiography 被估计。心肌的梗塞尺寸和 apoptosis 被检验。在心织物的全部的浆液 LDH 层次和 CK 活动, superoxide 生产, MDA 层次和抗氧化剂草皮活动是坚定的。一在里面 vitro 学习在胚胎的导出心肌层的房间 H9C2 暴露了到模仿的 ischemia/reperfusion (先生) 的有教养的老鼠上被执行。apoptotic 的表达式,嗯压力相关、发信号的蛋白质用西方的污点 analyses.Results 被估计: 有 BBR 的预告的处理显著地减少了 MI/R-induced 心肌的梗塞尺寸,改进心脏的函数,和压制的心肌的 apoptosis 和氧化损坏。而且,有 BBR 的预告的处理压制了 MI/R-induced 嗯应力,证实了由下面调整心肌的津贴和 eIF2α 的 phosphorylation 层次;并且在心纸巾的 ATF4 和砍的表示。有 BBR 的预告的处理也激活与 AG490 在心纸巾,和合作处理表明小径的 JAK2/STAT3,一个特定的 JAK2/STAT3 禁止者,在 MI/R-induced 上堵住了 BBR 的 BBR,而且抑制的不仅保护的效果嗯应力。在 H9C2 房间,有 BBR 的处理(50 μ mol/L ) 显著地减少的导致先生的房间 apoptosis,氧化应力并且嗯应力,被 transfection 与 JAK2 siRNA.Conclusion 废除, BBR 改善在由激活表明小径和 attenuating 的 AK2/STAT3 的老鼠的 MI/R 损害嗯导致压力的 apoptosis。 | Guo-long ZHAO Li-ming YU Wen-li GAO Wei-xun DUAN Bo JIANG Xu-dong LIU Bin ZHANG Zhen-hua LIU Meng-en ZHAI Zhen-xiao JIN Shi-qiang YU Yun WANG | 2016 | Acta Pharmacologica Sinica2016，37，3： | 47 |
| 3 | Erianin,a novel dibenzyl compound in Dendrobium extract,inhibits lung cancer cell growth and migration via calcium/calmodulin-dependent ferroptosis显示文摘Ferroptosis,a novel form of programmed cell death,is characterized by iron-dependent lipid peroxidation and has been shown to be involved in multiple diseases,including cancer.Stimulating ferroptosis in cancer cells may be a potential strategy for cancer therapy.Therefore,ferroptosis-inducing drugs are attracting more attention for cancer treatment.Here,we showed that erianin,a natural product isolated from Dendrobium chrysotoxum Lindl,exerted its anticancer activity by inducing cell death and inhibiting cell migration in lung cancer cells.Subsequently,we demonstrated for the first time that erianin induced ferroptotic cell death in lung cancer cells,which was accompanied by ROS accumulation,lipid peroxidation,and GSH depletion.The ferroptosis inhibitors Fer-1 and Lip-1 but not Z-VAD-FMK,CQ,or necrostatin-1 rescued erianin-induced cell death,indicating that ferroptosis contributed to erianin-induced cell death.Furthermore,we demonstrated that Ca^(2+)/CaM signaling was a critical mediator of erianin-induced ferroptosis and that blockade of this signaling significantly rescued cell death induced by erianin treatment by suppressing ferroptosis.Taken together,our data suggest that the natural product erianin exerts its anticancer effects by inducing Ca^(2+)/CaMdependent ferroptosis and inhibiting cell migration,and erianin will hopefully serve as a prospective compound for lung cancer treatment. | Peng Chen Qibiao Wu Jiao Feng Lili Yan Yitian Sun Shuiping Liu Yu Xiang Mingming Zhang Ting Pan Xiaying Chen Ting Duan Lijuan Zhai Bingtao Zhai Wengang Wang Ruonan Zhang Bi Chen Xuemeng Han Yicong Li Liuxi Chen Ying Liu Xingxing Huang Ting Jin Wenzheng Zhang Hong Luo Xiaohui Chen Yongqiang Li Qiujie Li Guohua Li Qin Zhang Lvjia Zhuo Zuyi Yang Huifen Tang Tian Xie Xiaoping Ouyang Xinbing Sui | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 42 |
| 4 | The Genome Sequence Archive Family: Toward Explosive Data Growth and Diverse Data Types显示文摘The Genome Sequence Archive(GSA)is a data repository for archiving raw sequence data,which provides data storage and sharing services for worldwide scientific communities.Considering explosive data growth with diverse data types,here we present the GSA family by expanding into a set of resources for raw data archive with different purposes,namely,GSA(http://gffzz77e3413bc06540edsx69owk9xkn0w6k0n.ffgz.tsg.suse.edu.cn/gsa/),GSA for Human(GSA-Human,http://gffzz77e3413bc06540edsx69owk9xkn0w6k0n.ffgz.tsg.suse.edu.cn/gsa-human/),and Open Archive for Miscellaneous Data(OMIX,http://gffzz77e3413bc06540edsx69owk9xkn0w6k0n.ffgz.tsg.suse.edu.cn/omix/).Compared with the 2017 version,GSA has been significantly updated in data model,online functionalities,and web interfaces.GSA-Human,as a new partner of GSA,is a data repository specialized in human genetics-related data with controlled access and security.OMIX,as a critical complement to the two resources mentioned above,is an open archive for miscellaneous data.Together,all these resources form a family of resources dedicated to archiving explosive data with diverse types,accepting data submissions from all over the world,and providing free open access to all publicly available data in support of worldwide research activities. | Tingting Chen Xu Chen Sisi Zhang Junwei Zhu Bixia Tang Anke Wang Lili Dong Zhewen Zhang Caixia Yu Yanling Sun Lianjiang Chi Huanxin Chen Shuang Zhai Yubin Sun Li Lan Xin Zhang Jingfa Xiao Yiming Bao Yanqing Wang Zhang Zhang Wenming Zhao | 2021 | Genomics, Proteomics & Bioinformatics2021，19，4： | 38 |
| 5 | Treatment of malignant biliary obstruction by combined percutaneous transhepatic biliary drainage with local tumor treatment显示文摘瞄准:为了评估本地肿瘤的用途,治疗为恶意的妨碍的胆汁的疾病把胆汁的排水(PTBD ) 与经皮的 transhepatic 相结合。方法:有恶意的胆汁的阻塞的 233 个病人的一个总数被金属性的 stents 或塑料试管的放置与 PTBD 在我们的医院里对待。在 PTBD 以后, 49 个病人接受了 brachytherapy 或额外放射的治疗或动脉的注入化疗。病人被跟随在上面与临床、 X 光线照相术的评估。幸存和斯滕特氏印模膏明显率被 Kaplan-Meier 幸存分析计算。结果: 22 个病人经历了化疗(肝的癌的 11 个盒子,胰腺的癌的 7 个盒子,变形淋巴结病的 4 个盒子),并且 14 个病人收到了放射疗法( cholangiocarcinoma 的 10 个盒子,胰腺的癌的 4 个盒子),并且 13 个病人接受了 brachytherapy ( cholangiocarcinoma 的 7 个盒子,胰腺的癌的 3 个盒子,变形淋巴结病的 4 个盒子)。在 1, 3, 6,和 12 个月的本地肿瘤治疗组的幸存率分别地是 97.96% , 95.92% , 89.80% ,和 32.59% ,比的长非治疗组。在 1, 3, 6,和 12 个月的明显率分别地是 97.96% , 93.86% , 80.93% ,和 56.52% 。明显率的差别不在治疗组之间是重要的,非,治疗组织。结论:我们的结果建议那本地肿瘤治疗能与恶意的胆汁的阻塞延长病人的生存时间,并且可以改进斯滕特氏印模膏明显。 | Xiao-Jun Qian Ren-You Zhai Ding-Ke Dai Ping Yu Li Gao | 2006 | World Journal of Gastroenterology2006，12，2： | 31 |
| 6 | Multi-objective optimization of a mixed-flow pump impeller using modified NSGA-II algorithm显示文摘In order to maintain a uniform distribution of pareto-front solutions, a modified NSGA-II algorithm coupled with a dynamic crowding distance(DCD) method is proposed for the multi-objective optimization of a mixed-flow pump impeller. With the pump meridional section fixed, ten variables along the shroud and hub are selected to control the blade load by using a three-dimensional inverse design method. Hydraulic efficiency, along with impeller head, is applied as an optimization objective; and a radial basis neural network(RBNN) is adopted to approximate the objective function with 82 training samples. Local sensitivity analysis shows that decision variables have different impacts on the optimization objectives. Instead of randomly selecting one solution to implement, a technique for ordering preferences by similarity to ideal solution(TOPSIS) is introduced to select the best compromise solution(BCS) from pareto-front sets. The proposed method is applied to optimize the baseline model, i.e. a mixed- flow waterjet pump whose specific speed is 508 min?1?m3s?1?m. The performance of the waterjet pump was experimentally tested. Compared with the baseline model, the optimized impeller has a better hydraulic efficiency of 92% as well as a higher impeller head at the design operation point. Furthermore, the off-design performance is improved with a wider highefficiency operation range. After optimization, velocity gradients on the suction surface are smoother and flow separations are eliminated at the blade inlet part. Thus, the authors believe the proposed method is helpful for optimizing the mixed-flow pumps. | HUANG RenFang LUO XianWu JI Bin WANG Peng YU An ZHAI ZhiHong ZHOU JiaJian | 2015 | Science China(Technological Sciences)2015，58，12： | 32 |
| 7 | Predictive value of the Chinese group on the study of severe hepatitis B-acute-on-chronic liver failure score in the short-term prognosis of patients with hepatitis B virus-related acute-on-chronic liver failure显示文摘Background:As a large,prospective,multicenter study-based prognostic score for hepatitis B virus-related acute-on-chronic liver failure(HBV-ACLF),the Chinese group on the study of severe hepatitis B-acute-on-chronic liver failure score(COSSH-ACLFs),has been approved by some foreign scholars;however,its predictive value needs to be verified.This study investigated the predictive value of COSSH-ACLFs for short-term prognosis in Chinese patients with HBV-ACLF.Methods:This retrospective cohort study included 751 patients with HBV-ACLF admitted to the Fifth Medical Center of Chinese PLA General Hospital between January 2011 and December 2014.Spearman method was used to assess the correlation of COSSHACLFs with classical scores.Different COX multivariate regression models were used to confirm the relationship between COSSHACLFs and short-term prognosis in patients with HBV-ACLF,and stratified analysis was used to further verify the stability of this relationship.We compared the predictive powers of COSSH-ACLFs and other classical scores using area under the receiver operating characteristic curve(AUROC)and Z-test.Results:A total of 975 patients with HBV-ACLF were screened,and 751 were analyzed(623 male and 128 female).COSSH-ACLFs was the highest in patients with end-stage ACLF,followed by those with middle-and early-stage ACLF(H=211.8,P<0.001).In the fully adjusted model,COX multivariate regression analysis revealed that COSSH-ACLFs(as a continuous variable)was independently and positively correlated with mortality risk in patients with HBV-ACLF at 28 days(hazard ratio[HR]:1.37[1.22,1.53],P<0.001)and 90 days(HR:1.43[1.29,1.58],P<0.001).The same trend could be observed in the crude model and minimally adjusted model.The AUROCs of COSSH-ACLFs for 28-day and 90-day prognoses in patients with HBV-ACLF were 0.807 and 0.792,respectively,indicating a stronger predictive accuracy than those of classic models.Conclusions:COSSH-ACLFs,with a superior predictive accuracy compared with other classical scores,can strongly predict shortterm prognosis in Chinese patients with HBV-ACLF. | Jing-Jing Tong Wei Zhao Xiu-Ying Mu Xiang Xu Hai-Bin Su Xiao-Yan Liu Jing Chen Xing-Ran Zhai Yu Wang Jin-Hua Hu | 2019 | Chinese Medical Journal2019，，13： | 27 |
| 8 | Safety threshold of high-speed railway pier settlement based on train-track-bridge dynamic interaction显示文摘This paper presents a method to determine the safety threshold of bridge pier settlement in high-speed railways.An analytical expression of the mapping relationship between the pier settlement and the rail deformation is derived theoretically for the double block ballastless track-bridge system.By adopting the superposition of the track random irregularity and the rail deformation caused by the pier settlement as the excitation inputs,the variations of vehicle dynamics indices with pier settlement are comparatively analyzed.Then,the safety threshold of the bridge pier settlement is obtained according to the limit of vehicle running safety and ride comfort indices of the high-speed trains.Results show that the dynamics indices of different trains have different sensitivities to the pier settlement,and the train CRH2C is the most sensitive one among all the types of Chinese high-speed trains.When passing through the bridges in common span with pier settlement at the speed of 250–350 km/h,the trains suffer the low-frequency excitations,and the vertical acceleration of car body is most sensitive to the pier settlement of all the dynamics indices.When the car body vertical acceleration just exceeds the allowable limit,the critical settlement value is 23.4 mm,which is much bigger than the pier differential settlement limit in the current code for Chinese high-speed railways. | CHEN Zhao Wei ZHAI Wan Ming CAI Cheng Biao SUN Yu | 2015 | Science China(Technological Sciences)2015，58，2： | 26 |
| 9 | Exploration of the formation mechanism and source attribution of ambient ozone in Chongqing with an observation-based model显示文摘An intensive field campaign was conducted in Chongqing during the summer of 2015 to explore the formation mechanisms of ozone pollution. The sources of ozone, the local production rates, and the controlling factors, as well as key species of volatile organic compounds(VOCs), were quantified by integrating a local ozone budget analysis, calculations of the relative incremental reactivity, and an empirical kinetic model approach. It was found that the potential for rapid local ozone formation exists in Chongqing. During ozone pollution episodes, the ozone production rates were found to be high at the upwind station Nan Quan, the urban station Chao Zhan, and the downwind station Jin-Yun Shan. The average local ozone production rate was 30×10^(-9) V/V h^(-1) and the daily integration of the produced ozone was greater than 180×10^(-9) V/V. High ozone concentrations were associated with urban and downwind air masses. At most sites, the local ozone production was VOC-limited and the key species were aromatics and alkene, which originated mainly from vehicles and solvent usage. In addition, the air masses at the northwestern rural sites were NO_x-limited and the local ozone production rates were significantly higher during the pollution episodes due to the increased NOx concentrations. In summary, the ozone abatement strategies of Chongqing should be focused on the mitigation of VOCs. Nevertheless, a reduction in NO_x is also beneficial for reducing the regional ozone peak values in Chongqing and the surrounding areas. | SU Rong LU KeDing YU JiaYan TAN ZhaoFeng JIANG MeiQing LI Jing XIE ShaoDong WU YuSheng ZENG LiMin ZHAI ChongZhi ZHANG YuanHang | 2018 | Science China Earth Sciences2018，61，1： | 24 |
| 10 | Isolation and characterization of dengue virus serotype 2 from the large dengue outbreak in Guangdong, China in 2014显示文摘Dengue has been well recognized as a global public health threat,but only sporadic epidemics and imported cases were reported in recent decades in China.Since July 2014,an unexpected large dengue outbreak has occurred in Guangdong province,China,resulting in more than 40000 patients including six deaths.To clarify and characterize the causative agent of this outbreak,the acute phase serum from a patient diagnosed with severe dengue was subjected to virus isolation and high-throughput sequencing(HTS).Traditional real-time RT-PCR and HTS with Ion Torrent PGM detected the presence of dengue virus serotype 2(DENV-2).A clinical DENV-2 isolate GZ05/2014 was obtained by culturing the patient serum in mosquito C6/36 cells.The complete genome of GZ05/2014 was determined and deposited in Gen Bank under the access number KP012546.Phylogenetic analysis based on the complete envelope gene showed that the newly DENV-2 isolate belonged to Cosmopolitan genotype and clustered closely with other Guangdong strains isolated in the past decade.No amino acid mutations that are obviously known to increase virulence or replication were identified throughout the genome of GZ05/2014.The high homology of Guangdong DENV-2 strains indicated the possibility of establishment of local DENV-2 circulation in Guangdong,China.These results help clarify the origin of this epidemic and predict the future status of dengue in China. | ZHAO Hui ZHAO Ling Zhai JIANG Tao LI Xiao Feng FAN Hang HONG Wen Xin ZHANG Yu ZHU Qin YE Qing TONG Yi Gang CAO Wu Chun ZHANG Fu Chun QIN Cheng Feng | 2014 | Science China(Life Sciences)2014，57，12： | 21 |
| 11 | Changes in Waist Circumference and Abdominal Obesity among Chinese Adults over a Ten-year Period显示文摘Objective The aim of this study was to describe changes in waist circumference(WC) and prevalence of abdominal obesity over a period of 10 years among Chinese adults in different socio-economic status(SES). Methods Data derived from the China Nutrition and Health Surveillance during 2002 and 2010-2012. We calculated the mean WC and the prevalence of abdominal obesity by gender, place of residence, SES indicators(education, income, and marital status), and body mass index(BMI) categoriesand used pooled t-tests to assess the differences between the two time periods. Results 26.0% of men and 25.3% of women had abdominal obesity in 2010-2012. The age-adjusted mean WC increased by 2.7 cm among men and 2.1 cm among women; the age-adjusted prevalence of abdominal obesity increased by 7.7% among men and 5.3% among women. The rising trends were observed in all subgroups except for a negative growth in high-income women. People living in rural areas with low education and income and with a BMI of 18.5 to 23.9 kg/m^2 had a greater absolute and relative increase in WC. People living in rural areas with low income had a greater relative increase in abdominal obesity. Conclusion The mean WC and prevalence of abdominal obesity among Chinese adults have increased during the past 10 years. Gender differences were noted using various SES indicators. | ZHAI Yi FANG Hong Yun YU Wen Tao YU Dong Mei ZHAO Li Yun LIANG Xiao Feng ZHAO Wen Hua | 2017 | Biomedical and Environmental Sciences2017，30，5： | 19 |
| 12 | Whole-genome sequencing of 508 patients identifies key molecular features associated with poor prognosis in esophageal squamous cell carcinoma显示文摘Esophageal squamous cell carcinoma(ESCC)is a poor-prognosis cancer type with limited understanding of its molecular etiology.Using 508 ESCC genomes,we identified five novel significantly mutated genes and uncovered mutational signature clusters associated with metastasis and patients’outcomes.Several functional assays implicated that NFE2L2 may act as a tumor suppressor in ESCC and that mutations in NFE2L2 probably impaired its tumor-suppressive function,or even conferred oncogenic activities.Additionally,we found that the NFE2L2 mutations were significantly associated with worse prognosis of ESCC.We also identified potential noncoding driver mutations including hotspot mutations in the promoter region of SLC35E2 that were correlated with worse survival.Approximately 5.9%and 15.2%of patients had high tumor mutation burden or actionable mutations,respectively,and may benefit from immunotherapy or targeted therapies.We found clinically relevant coding and noncoding genomic alterations and revealed three major subtypes that robustly predicted patients’outcomes.Collectively,we report the largest dataset of genomic profiling of ESCC useful for developing ESCC-specific biomarkers for diagnosis and treatment. | Yongping Cui Hongyan Chen Ruibin Xi Heyang Cui Yahui Zhao Enwei Xu Ting Yan Xiaomei Lu Furong Huang Pengzhou Kong Yang Li Xiaolin Zhu Jiawei Wang Wenjie Zhu Jie Wang Yanchun Ma Yong Zhou Shiping Guo Ling Zhang Yiqian Liu Bin Wang Yanfeng Xi Ruifang Sun Xiao Yu Yuanfang Zhai Fang Wang Jian Yang Bin Yang Caixia Cheng Jing Liu Bin Song Hongyi Li Yi Wang Yingchun Zhang Xiaolong Cheng Qimin Zhan Yanhong Li Zhihua Liu-Show | 2020 | Cell Research2020，30，10： | 19 |
| 13 | Vibration analysis and control technologies of hydraulic pipeline system in aircraft:A review显示文摘Vibrations in aircraft hydraulic pipeline system,due to multi-source excitation of high fluid pressure fluctuation and serious vibration environment of airframe,can cause the pipeline system vibration failures through overload in engineering field.Controlling the vibrations in hydraulic pipeline is a challenging work to ensure the flight safety of aircraft.The common vibration control technologies have been demonstrated to be effective in typical structures such as aerospace structures,shipbuilding structures,marine offshore structures,motor structures,etc.However,there are few research literatures on vibration control strategies of aircraft hydraulic pipeline.Combining with the development trend of aircraft hydraulic pipeline system and the requirement of vibration control technologies,this paper provides a detailed review on the current vibration control technologies in hydraulic pipeline system.A review of the general approaches following the passive and active control technologies are presented,which are including optimal layout technique of pipeline and clamps,constrained layer damping technique,vibration absorber technique,hydraulic hose technique,optimal pump structure technique,and active vibration control technique of pipeline system.Finally,some suggestions for the application of vibration control technologies in engineering field are given. | Peixin GAO Tao YU Yuanlin ZHANG Jiao WANG Jingyu ZHAI | 2021 | Chinese Journal of Aeronautics2021，34，4： | 19 |
| 14 | Expansion and differentiation of human hepatocyte-derived liver progenitor-like cells and their use for the study of hepatotropic pathogens显示文摘The study of pathophysiological mecha nisms in huma n liver disease has bee n constrained by the in ability to expa nd primary hepatocytes in vitro while maintaining proliferative capacity and metabolic function. We and others have previously shown that mouse mature hepatocytes can be con verted to liver progenitor-like cells in vitro with defined chemical factors. Here we describe a protocol achieving efficient conversion of human primary hepatocytes into liver progenitor-like cells (HepLPCs) through delivery of developmentally relevant cues, including NAD +-dependent deacetylase SIRT1 signaling. These HepLPCs could be expanded sign ifica ntly duri ng in vitro passage. The expa nded cells can readily be con verted back into metabolically functional hepatocytes in vitro and upon tran splantati on in vivo. Under three-dime nsional culture conditions, differentiated cells gen erated from HepLPCs regai ned the ability to support infect io n or reactivatio n of hepatitis B virus (HBV). Our work dem on strates the utility of the con versi on betwee n hepatocyte and liver proge nitor-like cells for studying HBV biology and an tiviral therapies. These fin dings will facilitate the study of liver diseases and regenerative medicine. | Gong-Bo Fu Wei-Jian Huang Min Zeng Xu Zhou Hong-Ping Wu Chang-Cheng Liu Han Wu Jun Weng Hong-Dan Zhang Yong-Chao Cai Charles Ashton Min Ding Dan Tang Bao-Hua Zhang Yi Gao Wei-Feng Yu Bo Zhai Zhi-Ying He Hong-Yang Wang He-Xin Yan | 2019 | Cell Research2019，29，1： | 18 |
| 15 | Exploration and research progress of shale gas in China显示文摘There are three types of shale gas resources in China.The resources are present in large amounts and are widely distributed.Marine facies,transitional facies and continental facies resources each account for a third.Based on resource distributions,there are many wells penetrated into the Sinian,Cambrian, Ordovieian,Silurian,Devonian,Carboniferous and Permian strata of the Yangtze plate and its periphery, the North China Craton and the Tarim Basin.Many years of exploration have indicated that the marine Silurian Longmaxi shale gas is widely distributed in south China and has been industrialized in its production in the Siehuan basin.The shale gas from the Cambrian Niutitang Formation and the Sinian Doushantuo Formation are important discoveries in Yichang,Hub ei and Zhenba,Shanxi.There are also shale gas resources found within transitional facies and continental facies in different areas in China.The 'two element enrichment theory'has been summarized during the exploration process of Silurian marine shale gas in the Sichuan Basin.In addition,horizontal drilling and fracturing technologies up to 3500 m in depth have been developed.Based on the understanding of shale gas accumulation in a complex tectonic zone outside the Sichnan basin,a preliminary summary of the formation of the 'converse fault syneline control reservoir'and 'paleo uplift control reservoir'model has been constructed.The dominant theory of 'Trinity'shale gas enrichment and the high yield of the 'deep water Lu Pengxiang sedimentary facies belt, structural preservation conditions and overpressure'is summarized.Guided by the above theories.Anyel well in Guizhou and Eyangyel well in Hubei were drilled.'Four storey'oil and shale 'gas is found in the Permian Qixia group,the Silurian Shiniulan Formation,the Longmaxi Formation and the Ordovician Baota Formation in Anyel well.Good shale gas has been gound in the Cambrian Niutitang formation inian Doushantuo formation in Eyangye lwell.This paper aims to summarize and review the main progress,theoretical technology and problems of shale gas exploration and development in recent years in China,and predicts the future exploration and development direction for shale gas and possible exploration areas. | Gang-yi Zhai Yu-fang Wang Zhi Zhou Shu-fang Yu Xiang-lin Chen Yun-xiao Zhang | 2018 | China Geology2018，1，2： | 15 |
| 16 | GSA:Genome Sequence Archive显示文摘With the rapid development of sequencing technologies towards higher throughput and lower cost, sequence data are generated at an unprecedentedly explosive rate. To provide an efficient and easy-to-use platform for managing huge sequence data, here we present Genome Sequence Archive(GSA; http://gffzzdbc7b6aaae734bddhx69owk9xkn0w6k0n.ffgz.tsg.suse.edu.cn/gsa or http://gffzz4eaf941a184140e3hx69owk9xkn0w6k0n.ffgz.tsg.suse.edu.cn), a data repository for archiving raw sequence data. In compliance with data standards and structures of the International Nucleotide Sequence Database Collaboration(INSDC), GSA adopts four data objects(Bio Project, Bio Sample,Experiment, and Run) for data organization, accepts raw sequence reads produced by a variety of sequencing platforms, stores both sequence reads and metadata submitted from all over the world,and makes all these data publicly available to worldwide scientific communities. In the era of big data, GSA is not only an important complement to existing INSDC members by alleviating the increasing burdens of handling sequence data deluge, but also takes the significant responsibility for global big data archive and provides free unrestricted access to all publicly available data in support of research activities throughout the world. | Yanqing Wang Fuhai Song Junwei Zhu Sisi Zhang Yadong Yang Tingting Chen Bixia Tang Lili Dong Nan Ding Qian Zhang Zhouxian Bai Xunong Dong Huanxin Chen Mingyuan Sun Shuang Zhai Yubin Sun Lei Yu Li Lan Jingfa Xiao Xiangdong Fang Hongxing Lei Zhang Zhang Wenming Zhao | 2017 | Genomics, Proteomics & Bioinformatics2017，15，1： | 13 |
| 17 | Network pharmacology-based prediction and verification of the molecular targets and pathways for schisandrin against cerebrovascular disease显示文摘AIM: To illuminate the molecular targets for schisandrin against cerebrovascular disease based on the combined methods of network pharmacology prediction and experimental verification. METHOD: A protein database was established through constructing the drug-protein network from literature mining data. The protein-protein network was built through an in-depth exploration of the relationships between the proteins. The computational platform was implemented to predict and extract the sensitive sub-network with significant P-values from the protein-protein network. Then the key targets and pathways were identified from the sensitive sub-network. The most related targets and pathways were also confirmed in hydrogen peroxide(H2O2)-induced PC12 cells by Western blotting. RESULTS: Twelve differentially expressed proteins(gene names: NFKB1, RELA, TNFSF10, MAPK1, CHUK, CASP8, PIGS2, MAPK14, CREB1, IFNG, APP, and BCL2) were confirmed as the central nodes of the interaction network(45 nodes, 93 edges). The NF-κB signaling pathway was suggested as the most related pathway of schisandrin for cerebrovascular disease. Furthermore, schisandrin was found to suppress the expression and phosphorylation of IKKα, as well as p50 and p65 induced by H2O2 in PC12 cells by Western blotting. CONCLUSION: The computational platform that integrates literature mining data, protein-protein interactions, sensitive sub-network, and pathway results in identification of the NF-κB signaling pathway as the key targets and pathways for schisandrin. | LV Yan-Ni LI Shao-Xia ZHAI Ke-Feng KOU Jun-Ping YU Bo-Yang | 2014 | Chinese Journal of Natural Medicines2014，12，4： | 12 |
| 18 | Effects of subchronic oral toxic metal exposure on the intestinal microbiota of mice显示文摘Oral exposure to toxic metals such as cadmium(Cd), lead(Pb), copper(Cu) and aluminum(Al) can induce various adverse health effects in humans and animals. However, the effects of these metals on the gut microbiota have received limited attention. The present study demonstrated that long-term toxic metal exposure altered the intestinal microbiota of mice in a metal-specific and time-dependent manner.Subchronic oral Cu exposure for eight weeks caused a profound decline in gut microbial diversity in mice,whereas no significant changes were observed in groups treated with other metals. Cd exposure significantly increased the relative abundances of organisms from the genera Alistipes and Odoribacter and caused marked decreases in Mollicutes and unclassified Ruminococcaceae. Pb exposure significantly decreased the abundances of eight genera: unclassified and uncultured Ruminococcaceae, unclassified Lachnospiraceae, Ruminiclostridium_9, Rikenellaceae_RC9_gut_group, Oscillibacter, Anaerotruncus and Lachnoclostridium. Cu exposure affected abundances of the genera Alistipes, Bacteroides,Ruminococcaceae_UCG-014, Allobaculum, Mollicutes_RF9_norank, Rikenellaceae_RC9_gut_group,Ruminococcaceae_unclassified and Turicibacter. Al exposure increased the abundance of Odoribacter and decreased that of Anaerotruncus. Exposure to any metal for eight weeks significantly decreased the abundance of Akkermansia. These results provide a new understanding regarding the role of toxic metals in the pathogenesis of intestinal and systemic disorders in the host within the gut microbiota framework. | Qixiao Zhai Tianqi Li Leilei Yu Yue Xiao Saisai Feng Jiangping Wu Jianxin Zhao Hao Zhang Wei Chen | 2017 | Science Bulletin2017，62，12： | 12 |
| 19 | Comparative Transcriptional Profiling and Preliminary Study on Heterosis Mechanism of Super-Hybrid Rice显示文摘杂种优势比也是从二父母表演的后代改善了的生物现象和优异性能生来的父母线。混合米饭是在利用杂种优势的庄稼的最成功的 apotheoses 之一。由基因表示(半自动地面防空系统)的连续分析 F1 超级混血儿的米饭 Liangyou-2186 和它的父母介绍的 Transcriptional 表明 1183 差别表示了基因( DG ),在哪个之中 DG 被发现显著地在象光合作用和碳固定那样的小径充实,并且大多数涉及碳固定小径的关键基因在 F1 混合米饭展出了起来调整的表示。而且,相应的酶的增加的分解代谢的活动和光合的效率也被检测,它联合了显示那碳固定可能在 F1 混血儿,和力量被提高在超级混血儿的米饭与收益活力和杂种优势被联系。由有收益相关的量的特点 loci (QTL ) 的相关 DG,在微分基因表示和 phenotypic 变化之间的一种潜在的关系也被发现。另外,当以前在 Arabidopsis 报导了,包含生理节奏节奏、轻的发信号的小径的一个规章的网络也被发现,它建议如此的一个网络可能也与在混合米饭的杂种优势是相关的。总的来说,现在的学习为理解在米饭位于杂种优势下面的分子的机制提供另一个看法。 | Gui-Sheng Song Hong-Li Zhai Yong-Gang Peng Lei Zhang Gang Wei Xiao- Ying Chen Yu-Guo Xiao Lili Wang Yue-Jun Chen Bin Wu Bin Chen Yu Zhang Hua Chen Xiu-Jing Feng Wan-Kui Gong Yao Liu Zhi-Jie Yin Feng Wang Guo-Zhen Liu Hong-Lin Xu Xiao-Li Wei Xiao-Ling Zhao Pieter B.F. Ouwerkerk Thomas Hankemeier Theo Reijmers Rob van der Heijden Cong-Ming Lu Mei Wang Jan van der Greef Zhen Zhu | 2010 | Molecular Plant2010，3，6： | 12 |
| 20 | The evolution of the Huangling uplift and its control on the accumulation and preservation of shale gas显示文摘There are plenty of Sinian and Cambrian potential shale gas resources in South China,which is characterized by high thermal evolution degrees,poor drilling performances and only occurs in local areas.Taking the principle “high to find low” is the key issue to achieving a breakthrough in older shale.China Geological Survey drilled in the periphery of the Proterozoic basement,i.e.the Huangling anticline,in the western Hubei,and Hannan paleocontinent in the southern Shanxi.It received high-quality gas-bearing shale with relatively low Ro in the in Lower Cambrian Niutitang formation and Sinian Doushantuo formation.Based on geological conditions of shale gas reservoirs in the Huangling anticline,this paper puts forward the new model named “Control over reservoirs by periphery of basement” about shale gas accumulation,suggesting that the shale deposited in a deepwater continental shelf in the periphery of the basement is characterized by shallow burial,a short burial time,stable tectonics,relatively low thermal evolution degrees,and shale gas reservoirs in a good condition.The shale of the Sinian-Cambrian strata deposited in deepwater continental shelves in the periphery of Chuanzhong paleo-uplift in Sichuan,Hannan paleocontinent in the southern Shanxi,Huangling anticline in western Hubei and Jiangnang-Xuefeng paleo-uplift in Hunan and Guizhou province have good shale gas exploration potential. | Shu-jing Bao Gang-yi Zhai Zhi Zhou Shu-fang Yu Ke Chen Yu-fang Wang Hao Wang Yi-min Liu | 2018 | China Geology2018，1，3： | 9 |
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