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| 1 | The chromosome-level reference genome assembly for Panax notoginseng and insights into ginsenoside biosynthesis显示文摘Panax notoginseng,a perennial herb of the genus Panax in the family Araliaceae,has played an important role in clinical treatment in China for thousands of years because of its extensive pharmacological effects.Here,we report a high-quality reference genome of P.notoginseng,with a genome size up to 2.66 Gb and a contig N50 of 1.12 Mb,produced with third-generation PacBio sequencing technology.This is the first chromosome-level genome assembly for the genus Panax.Through genome evolution analysis,we explored phylogenetic and whole-genome duplication events and examined their impact on saponin biosynthesis.We performed a detailed transcriptional analysis of P.notoginseng and explored genelevel mechanisms that regulate the formation of characteristic tubercles.Next,we studied the biosynthesis and regulation of saponins at temporal and spatial levels.We combined multi-omics data to identify genes that encode key enzymes in the P.notoginseng terpenoid biosynthetic pathway.Finally,we identified five glycosyltransferase genes whose products catalyzed the formation of different ginsenosides in P.notoginseng.The genetic information obtained in this study provides a resource for further exploration of the growth characteristics,cultivation,breeding,and saponin biosynthesis of P.notoginseng. | Zhouqian Jiang Lichan Tu Weifei Yang Yifeng Zhang Tianyuan Hu Baowei Ma Yun Lu Xiuming Cui Jie Gao Xiaoyi Wu Yuru Tong Jiawei Zhou Yadi Song Yuan Liu Nan Liu Luqi Huang Wei Gao | 2021 | Plant Communications2021，2，1： | 11 |
| 2 | Molecular cloning and functional identification of sterol C24-methyltransferase gene from Tripterygium wilfordii显示文摘Sterol C24-methyltransferase(SMT) plays multiple important roles in plant growth and development. SMT1, which belongs to the family of transferases and transforms cycloartenol into 24-methylene cycloartenol, is involved in the biosynthesis of 24-methyl sterols. Here, we report the cloning and characterization of a cDNA encoding a sterol C24-methyltransferase from Tripterygium wilfordii(Tw SMT1). Tw SMT1(Gen Bank access number KU885950) is a 1530 bp cDNA with a 1041 bp open reading frame predicted to encode a 346-amino acid, 38.62 k Da protein. The polypeptide encoded by the SMT1 cDNA was expressed and purified as a recombinant protein from Escherichia coli(E. coli) and showed SMT activity. The expression of Tw SMT1 was highly up-regulated in T. wilfordii cell suspension cultures treated with methyl jasmonate(Me JA). Tissue expression pattern analysis showed higher expression in the phellem layer compared to the other four organs(leaf, stem, xylem and phloem), which is about ten times that of the lowest expression in leaf. The results are meaningful for the study of sterolbiosynthesis of T. wilfordii and will further lay the foundations for the research in regulating both the content of other main compounds and growth and development of T. wilfordii. | Hongyu Guan Yujun Zhao Ping Su Yuru Tong Yujia Liu Tianyuan Hu Yifeng Zhang Xianan Zhang Jia Li Xiaoyi Wu Luqi Huang Wei Gao | 2017 | Acta Pharmaceutica Sinica B2017，7，5： | 4 |
| 3 | Mechanistic analysis for the origin of diverse diterpenes in Tripterygium wilfordii显示文摘Tripterygium wilfordii is a valuable medicinal plant rich in biologically active diterpenoids,but there are few studies on the origins of these diterpenoids in its secondary metabolism.Here,we identified three regions containing tandemly duplicated diterpene synthase genes on chromosomes(Chr) 17 and 21 of T. wilfordii and obtained 11 diterpene synthases with different functions.We farther revealed that these diterpene synthases underwent duplication and rearrangement at approximately 2.3-23.7 million years ago(MYA) by whole-genome triplication(WGT),transposon mediation,and tandem duplication,followed by functional divergence.We first demonstrated that four key amino acids in the sequences of TwCPS3,TwCPS5,and TwCPSS were altered during evolution,leading to their functional divergence and the formation of diterpene secondary metabolites.Then,we demonstrated that the functional divergence of three TwKSLs was driven by mutations in two key amino acids.Finally,we discovered the mechanisms of evolution and pseudogenization of miltiradiene synthases in T.wilfordii and elucidated that the new function in TwMS1/2 from the terpene synthase(TPS)-b subfamily was caused by progressive changes in multiple amino acids after the WGT event.Our results provide key evidence for the formation of diverse diterpenoids during the evolution of secondary metabolites in T.wilfordii. | Lichan Tu Xinbo Cai Yifeng Zhang Yuru Tong Jian Wang Ping Su Yun Lu Tianyuan Hu Yunfeng Luo Xiaoyi Wu Dan Li Luqi Huang Wei Gao | 2022 | Acta Pharmaceutica Sinica B2022，12，6： | 1 |
| 4 | Adding power of artificial intelligence to situational awareness of large interconnections dominated by inverter‐based resources显示文摘Large‐scale power systems exhibit more complex dynamics due to the increasing inte-gration of inverter‐based resources(IBRs).Therefore,there is an urgent need to enhance the situational awareness capability for better monitoring and control of power grids dominated by IBRs.As a pioneering Wide‐Area Measurement System,FNET/GridEye has developed and implemented various advanced applications based on the collected synchrophasor measurements to enhance the situational awareness capability of large‐scale power grids.This study provides an overview of the latest progress of FNET/GridEye.The sensors,communication,and data servers are upgraded to handle ultra‐high density synchrophasor and point‐on‐wave data to monitor system dynamics with more details.More importantly,several artificial intelligence(AI)‐based advanced appli-cations are introduced,including AI‐based inertia estimation,AI‐based disturbance size and location estimation,AI‐based system stability assessment,and AI‐based data authentication. | Lin Zhu Yinfeng Zhao Yi Cui Shutang You Wenpeng Yu Shengyuan Liu He Yin Chang Chen Yuru Wu Wei Qiu Mirka Mandich Hongyu Li Adedasola Ademola Chengwen Zhang Chujie Zeng Xinlan Jia Weikang Wang Haoyu Yuan Huaiguang Jiang Jin Tan Yilu Liu | 2021 | High Voltage2021，6，6： | 0 |
| 5 | LDL receptor-related protein 1 (LRP1),a novel target for opening the blood-labyrinth barrier(BLB)显示文摘Inner ear disorders are a cluster of diseases that cause hearing loss in more than 1.5 billion people worldwide.However,the presence of the blood-labyrinth barrier(BLB)on the surface of the inner ear capillaries greatly hinders the effectiveness of systemic drugs for prevention and intervention due to the low permeability,which restricts the entry of most drug compounds from the bloodstream into the inner ear tissue.Here,we report the finding of a novel receptor,low-density lipoprotein receptor-related protein 1(LRP1),that is expressed on the BLB,as a potential target for shuttling therapeutics across this barrier.As a proof-ofconcept,we developed an LRP1-binding peptide,IETP2,and covalently conjugated a series of model small-molecule compounds to it,including potential drugs and imaging agents.All compounds were successfully delivered into the inner ear and inner ear lymph,indicating that targeting the receptor LRP1 is a promising strategy to enhance the permeability of the BLB.The discovery of the receptor LRP1 will illuminate developing strategies for crossing the BLB and for improving systemic drug delivery for inner ear disorders. | Xi Shi Zihao Wang Wei Ren Long Chen Cong Xu Menghua Li Shiyong Fan Yuru Xu Mengbing Chen Fanjun Zheng Wenyuan Zhang Xinbo Zhou Yue Zhang Shiwei Qiu Liyuan Wu Peng Zhou Xinze Lv Tianyu Cui Yuehua Qiao Hui Zhao Weiwei Guo Wei Chen Song Li Wu Zhong Jian Lin Shiming Yang | 2022 | Signal Transduction and Targeted Therapy2022，7，7： | 0 |
| 6 | The effect of secondary structures on the generation of characteristic events during the translocation of DNA hybrid through ?-hemolysin显示文摘Nanopore has been developed to be a powerful,single-molecule analytical tool for sensing ions,small organic molecules and biomacromolecules such as proteins and DNAs.Generally,the identity of the analyte can be revealed by current amplitude changes and mean dwell time of the analyte binding events.In some cases,generation of highly characteristic current events affords an alternative way of analyte determination with high confidence level.However,we found that secondary structures in DNA/RNA hybrids might severely hinder the generation of signature events during their translocation through?-hemolysin nanopore.In this report,we propose a strategy to add a certain concentration of urea in the buffer solution for single channel recordings and validate that low concentration of urea can effectively denature the secondary structures in DNA hybrids and recover the generation of signature events.This finding might be useful in other secondary structure-related nanopore sensing activities. | Xingyong Wang Yuru Li Ting Li Lei Liu Hai-Chen Wu | 2016 | Science China Chemistry2016，59，1： | 0 |
| 7 | A new method for preparing a rat intracerebral hemorrhage model by combining focused ultrasound and microbubbles显示文摘Background:We aimed to prepare a non-invasive,reproducible,and controllable rat model of intracerebral hemorrhage with focused ultrasound(FUS).Methods:A rat intracerebral hemorrhage(ICH)model was established by combining FUS and microbubbles(μBs),and edaravone was used to verify whether the free radical scavenger had a protective effect on the model.The brain tissue of each group was sectioned to observe the gross histology,blood-brain barrier(BBB)permeability,cerebral infarction volume,and histopathological changes.Results:Compared with the FUS group,the BBB permeability was significantly increased in the FUS+μBs(F&B)group(p=0.0021).The second coronal slice in the F&B group had an obvious hemorrhage lesion,and the FUS+μBs+edaravone(F&B&E)group had smaller hemorrhage areas;however,ICH did not occur in the FUS group.The cerebral infarction volume in the F&B group was significantly larger than that in the FUS group(p=0.0030)and F&B&E group(p=0.0208).HE staining results showed that nerve fibrinolysis,neuronal necrosis,microglia production,and erythrocytes were found in both the F&B group and the F&B&E group,but the areas of the nerve fibrinolysis and neuronal necrosis in the F&B group were larger than the F&B&E group.Conclusions:A rat ICH model was successfully prepared using theμBs assisted FUS treatment,and edaravone had a therapeutic effect on this model.This model can be used to study the pathophysiological mechanism of ICH-related diseases and in preclinical research on related new drugs. | Yao He Jie Yang Fengying Hu Min Liao Yuru Nie Xiaoxia Zhu Tao Zhang Keer Song Qinxi Li Xiaojie Li Chenghan Mei Zhe Wu Qiang Lu Zhihui Zhong | 2023 | Animal Models and Experimental Medicine2023，6，2： | 0 |
| 8 | Effect of carbon coating on electrochemical properties of AB_(3.5)-type La-Y-Ni-based hydrogen storage alloys显示文摘The superlattice La-Y-Ni-based hydrogen storage alloys have high discharge capacity and are easy to prepare.However,there is still a gap in commercial applications because of the severe corrosion of the alloys in electrolyte and poor high-rate dischargeability(HRD).Therefore,(LaSmY)(NiMnAl)_(3.5) alloy was prepared by magnetic levitation induction melting,and then the alloy was coated with different contents(0.1 wt%-1.0 wt%) of nano-carbons by low-temperature sintering with sucrose as the carbon source in this work.The results show that the cyclic stability and HRD of the alloy first increase and then decrease with the increase of carbon contents.The kinetic results show that the electrocatalytic activity and conductivity of the alloy electrodes can be enhanced by carbon coating.The electrochemical properties of the alloy are the best when the carbon coating content is 0.3 wt%.Compared with the uncoated alloy,the maximum discharge capacity(C_(max)) improves from 354.5 to 359.0 mAh/g,the capacity retention rate after 300 cycles(S_(300)) enhances from 73.15% to 80.01%,and the HRD_(1200) of the alloy enhances from 74.39% to 74.39%. | Ran Wu Huiping Yuan Yuru Liu Zhenyu Hou Zhinian Li Shumao Wang Lijun Jiang Lei Hao | 2022 | Journal of Rare Earths2022，40，8： | 0 |
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