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| 1 | Single cell analysis reveals inhibition of angiogenesis attenuates the progression of heterotopic ossification in Mkx^(−/−)mice显示文摘Tendon heterotopic ossification(HO)is characterized by bone formation inside tendon tissue,which severely debilitates people in their daily life.Current therapies fail to promote functional tissue repair largely due to our limited understanding of HO pathogenesis.Here,we investigate the pathological mechanism and propose a potential treatment method for HO.Immunofluorescence assays showed that the Mohawk(MKX)expression level was decreased in human tendon HO tissue,coinciding with spontaneous HO and the upregulated expression of osteochondrogenic and angiogenic genes in the tendons of Mkx^(−/−)mice.Single-cell RNA sequencing analyses of wild-type and Mkx^(−/−)tendons identified three cell types and revealed the excessive activation of osteochondrogenic genes during the tenogenesis of Mkx^(−/−)tendon cells.Single-cell analysis revealed that the gene expression program of angiogenesis,which is strongly associated with bone formation,was activated in all cell types during HO.Moreover,inhibition of angiogenesis by the small-molecule inhibitor BIBF1120 attenuated bone formation and angiogenesis in the Achilles tendons of both Mkx mutant mice and a rat traumatic model of HO.These findings provide new insights into the cellular mechanisms of tendon HO and highlight the inhibition of angiogenesis with BIBF1120 as a potential treatment strategy for HO. | Junxin Lin Yuwei Yang Wenyan Zhou Chao Dai Xiao Chen Yuanhao Xie Shan Han Huanhuan Liu Yejun Hu Chenqi Tang Varitsara Bunpetch Dandan Zhang Yishan Chen Xiaohui Zou Di Chen Wanlu Liu Hongwei Ouyang | 2022 | Bone Research2022，10，4： | 1 |
| 2 | Monitoring NAD(H) and NADP(H) dynamics during organismal development with genetically encoded fluorescent biosensors显示文摘Cell metabolism plays vital roles in organismal development,but it has been much less studied than transcriptional and epigenetic control of developmental programs.The difficulty might be largely attributed to the lack of in situ metabolite assays.Genetically encoded fluorescent sensors are powerful tools for noninvasive metabolic monitoring in living cells and in vivo by highly spatiotemporal visualization.Among all living organisms,the NAD(H)and NADP(H)pools are essential for maintaining redox homeostasis and for modulating cellular metabolism.Here,we introduce NAD(H)and NADP(H)biosensors,present example assays in developing organisms,and describe promising prospects for how sensors contribute to developmental biology research. | Ting Li Yejun Zou Shuning Liu Yi Yang Zhuo Zhang Yuzheng Zhao | 2022 | Cell Regeneration2022，11，1： | 0 |
| 3 | 代谢成像揭示白血病起始细胞归巢于骨内膜微环境并有效对称分裂的独特偏好显示文摘文章简介白血病起始细胞(leukemia-initiatingcells,LICs)在不同骨髓微环境中的代谢特征及其与细胞命运决定的关系尚不清楚。研究团队利用遗传编码的高度灵敏的NADH/NAD+代谢感受器(So Nar)标记和指示小鼠和患者白血病细胞代谢状态,构建了实时在体代谢成像体系,并阐明了白血病细胞中存在明显的代谢异质性。 | Xiaoxin Hao Hao Gu Chiqi Chen Dan Huang Yuzheng Zhao Li Xie Yejun Zou Hui Sophie Shu Yaping Zhang Xiaoxiao He Xiaoyun Lai Xiaocui Zhang Bo OZhou Cheng Cheng Zhang Guo-Qiang Chen 于卓 杨弋 郑俊克 | 2020 | 科学新闻2020，，2： | 0 |
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