|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Clinical and biochemical indexes from 2019-nCoV infected patients linked to viral loads and lung injury显示文摘The outbreak of the 2019-nCoV infection began in December 2019 in Wuhan,Hubei province,and rapidly spread to many provinces in China as well as other countries.Here we report the epidemiological,clinical,laboratory,and radiological characteristics,as well as potential biomarkers for predicting disease severity in 2019-nCoV-infected patients in Shenzhen,China.All 12 cases of the 2019-nCoV-infected patients developed pneumonia and half of them developed acute respiratory distress syndrome (ARDS).The most common laboratory abnormalities were hypoalbuminemia,lymphopenia,decreased percentage of lymphocytes (LYM) and neutrophils (NEU),elevated C-reactive protein (CRP) and lactate dehydrogenase (LDH),and decreased CD8 count.The viral load of 2019-nCoV detected from patient respiratory tracts was positively linked to lung disease severity.ALB,LYM,LYM (%),LDH,NEU (%),and CRP were highly correlated to the acute lung injury.Age,viral load,lung injury score,and blood biochemistry indexes,albumin (ALB),CRP,LDH,LYM (%),LYM,and NEU (%),may be predictors of disease severity.Moreover,the Angiotensin Ⅱlevel in the plasma sample from 2019-nCoV infected patients was markedly elevated and linearly associated to viral load and lung injury.Our results suggest a number of potential diagnosis biomarkers and angiotensin receptor blocker (ARB) drugs for potential repurposing treatment of 2019-nCoV infection. | Yingxia Liu Yang Yang Cong Zhang Fengming Huang Fuxiang Wang Jing Yuan Zhaoqin Wang Jinxiu Li Jianming Li Cheng Feng Zheng Zhang Lifei Wang Ling Peng Li Chen Yuhao Qin Dandan Zhao Shuguang Tan Lu Yin Jun Xu Congzhao Zhou Chengyu Jiang Lei Liu | 2020 | Science China(Life Sciences)2020，63，3： | 157 |
| 2 | Surveillance, epidemiology, and pathogen spectrum of hand, foot, and mouth disease in mainland of China from 2008 to 2017显示文摘Hand,foot and mouth disease(HFMD)was reported in May 2,2008 to be the 38th legally notifiable disease in China's National Notifiable Disease Reporting and Surveillance System.In order to solve the infection,an extensive three-level HFMD surveillance laboratory network was established.In this study,the framework of that network is assessed and the incidence of HFMD in China from 2008 to 2017 is reported using a descriptive epidemiologic method.During these 10 years,a series of techniques have been widely applied in all the network laboratories.Using information and material obtained from the network,a virus bank and database containing 18,238 viruses were established.Nationally,18,184,834 HFMD cases,including 152,436 severe cases and 3633 fatal cases,were reported in mainland of China.The average annual incidence in the population was 133.99/100,000 people,with a maximum incidence of 205.06/100,000 people in 2014.The incidence and mortality rates of HFMD were the highest in children aged 1–2 years.The numbers of reported cases fluctuated,with a high incidence observed every 2 years.An overall increase in the number of reported cases was also observed throughout the study period.Despite this,the incidence of severe cases and the mortality rate have been decreasing.High-risk regions are located in southern,eastern,and central China.Two peaks of HFMD infection cases were observed annually except for Northeast China.Different proportions of enterovirus serotypes were observed during the studied years.The predominant enterovirus varies from year to year,but the disease severity is always closely related to the specific serotype.EV-A71 is the dominant serotype associated with severe and fatal cases,with constituent ratios of 70.03%and 92.23%,respectively.The studied highly sensitive and efficient surveillance network provides information that is critical for prevention and control of the disease.It is extremely necessary and important to continuously conduct extensive virological surveillance for HFMD. | Tianjiao Ji Taoli Han Xiaojuan Tan Shuangli Zhu Dongmei Yan Qian Yang Yang Song Aili Cui Yan Zhang Naiying Mao Songtao Xu Zhen Zhu Dandan Niu Yong Zhang Wenbo Xu | 2019 | Biosafety and Health2019，1，1： | 26 |
| 3 | Plant-derived phosphocholine facilitates cellular uptake of anti-pulmonary fibrotic HJT-sRNA-m7显示文摘Pulmonary fibrosis, a progressive chronic disease with a high mortality rate, has limited treatment options. Currently, lung transplantation remains the only effective treatment. Here we report that a small RNA, HJT-sRNA-m7, from a Chinese herbal medicine Hong Jing Tian(HJT, RHODIOHAE CRENULATAE RADIX ET RHIZOMA, Rhodiola crenulata) can effectively reduce the expressions of fibrotic hallmark genes and proteins both in alveolar in vitro and in mouse lung tissues in vivo. We also discovered over one hundred oil-soluble chemicals from HJT decoctions, most of which are found in lipid extracts from other Chinese herbals decoctions, including Pu Gong Ying(PGY, TARAXACI HERBA, Taraxacum mongolicum), Chuan Xin Lian(CXL, changed to 'ANDROGRAPHIS HERBA, Andrographis paniculata'), and Jin Yin Hua(JYH, lonicera japonica or Honeysuckle). We identified the active component in these decoctions as two forms of phosphocholines, PC(18:0/18:2) and PC(16:0/18:2). These PCs potentially could form liposomes with small RNAs to enter human alveolar and gastric cells. Our experimental results suggest an unprecendent lipid complex route through which botanic small RNA can enter human bodies.Our results provide an innovative treatment strategy for oral delivery of siRNAs as therapeutic medication. | Jianchao Du Zhu Liang Jiantao Xu Yan Zhao Xiaoyun Li Yanli Zhang DANDan Zhao Ruxuan Chen Yang Liu Trupti Joshi Jiahui Chang Zhiqing Wang Yanxu Zhang Jindong Zhu Qiang Liu Dong Xu Chengyu Jiang | 2019 | Science China(Life Sciences)2019，62，3： | 21 |
| 4 | Gain-of-function mutant p53 in cancer progression and therapy显示文摘p53 is a key tumor suppressor,and loss of p53 function is frequently a prerequisite for cancer development.The p53 gene is the most frequently mutated gene in human cancers;p53 mutations occur in>50%of all human cancers and in almost every type of human cancers.Most of p53 mutations in cancers are missense mutations,which produce the full-length mutant p53(mutp53)protein with only one amino acid difference from wild-type p53 protein.In addition to loss of the tumor-suppressive function of wild-type p53,many mutp53 proteins acquire new oncogenic activities independently of wild-type p53 to promote cancer progression,termed gain-of-function(GOF).Mutp53 protein often accumulates to very high levels in cancer cells,which is critical for its GOF.Given the high mutation frequency of the p53 gene and the GOF activities of mutp53 in cancer,therapies targeting mutp53 have attracted great interest.Further understanding the mechanisms underlying mutp53 protein accumulation and GOF will help develop effective therapies treating human cancers containing mutp53.In this review,we summarize the recent advances in the studies on mutp53 regulation and GOF as well as therapies targeting mutp53 in human cancers. | Cen Zhang Juan Liu Dandan Xu Tianliang Zhang Wenwei Hu Zhaohui Feng | 2020 | Journal of Molecular Cell Biology2020，12，9： | 18 |
| 5 | Celastrol induces ferroptosis in activated HSCs to ameliorate hepatic fibrosis via targeting peroxiredoxins and HO-1显示文摘Ferroptosis is a form of regulated cell death, characterized by excessive membrane lipid peroxidation in an iron-and ROS-dependent manner. Celastrol, a natural bioactive triterpenoid extracted from Tripterygium wilfordii, shows effective anti-fibrotic and anti-inflammatory activities in multiple hepatic diseases. However, the exact molecular mechanisms of action and the direct protein targets of celastrol in the treatment of liver fibrosis remain largely elusive. Here, we discover that celastrol exerts anti-fibrotic effects via promoting the production of reactive oxygen species(ROS) and inducing ferroptosis in activated hepatic stellate cells(HSCs). By using activity-based protein profiling(ABPP) in combination with bio-orthogonal click chemistry reaction and cellular thermal shift assay(CETSA), we show that celastrol directly binds to peroxiredoxins(PRDXs), including PRDX1, PRDX2, PRDX4 and PRDX6,through the active cysteine sites, and inhibits their anti-oxidant activities. Celastrol also targets to heme oxygenase 1(HO-1) and upregulates its expression in activated-HSCs. Knockdown of PRDX1, PRDX2,PRDX4, PRDX6 or HO-1 in HSCs, to varying extent, elevated cellular ROS levels and induced ferroptosis. Taken together, our findings reveal the direct protein targets and molecular mechanisms via which celastrol ameliorates hepatic fibrosis, thus supporting the further development of celastrol as a promising therapeutic agent for liver fibrosis. | Piao Luo Dandan Liu Qian Zhang Fan Yang Yin-Kwan Wong Fei Xia Junzhe Zhang Jiayun Chen Ya Tian Chuanbin Yang Lingyun Dai Han-Ming Shen Jigang Wang | 2022 | Acta Pharmaceutica Sinica B2022，12，5： | 14 |
| 6 | Development and application of mechanized maize harvesters显示文摘Maize harvesting is one of the most important filed operations of maize production.As the accelerating development of maize industry,mechanized maize harvesting is widely accepted and used by farmers in the world.According to the harvesting methods,maize harvesters could be classified into two types,one is maize-for-grain harvesters,including pickers and grain harvesters,the other is whole plant harvesters,including forage harvesters and combined grain-stover harvesters.Structure characteristics,appropriate areas and relative technologies of those harvesters are described in this paper,i.e.,pickers are suitable for multi-crop areas,Grain harvesters are mainly for one-crop areas when grain moisture content is lower than 25%,combined grain-stover harvesters are applicable in agro-pastoral ecotone,and forage harvesters are capable in large-scaled animal husbandry farming areas where large amount of silage are required.Meanwhile,the future development trend of the different harvesters is predicted.Big horsepower engines,enhanced working efficiency,automation and intelligence are required by large scaled farms.As maize breeding development,more varieties that are suitable for grain harvesting are put into practice,so grain combine harvesters will be popular in future. | Yang Li Cui Tao Qu Zhe Li Kehong Yin Xiaowei Han Dandan Yan Bingxin Zhao Dongyue Zhang Dongxing | 2016 | International Journal of Agricultural and Biological Engineering2016，9，3： | 13 |
| 7 | Using borehole core analysis to reveal Late Quaternary paleoearthquakes along the Nankou-Sunhe Fault,Beijing显示文摘The Nankou-Sunhe Fault is a buried active normal fault that traverses the urban area of Beijing.Its seismic risks have caused considerable concerns.This paper studies paleoearthquakes along this fault by analyzing and correlating bore-hole cores obtained from triple-tube coring,incorporating experience acquired from trenching.As a result,a model for identifying earthquake-derived colluvium by sediment-core analysis is proposed.Triple-tube coring technique is useful to collect continuous undis-turbed soil core near the Nankou-Sunhe Fault.By identifying fault-scarp colluviums,determining cumulative displacement,and analysing stratum thickening on the hanging wall,we are able to establish a preliminary paleoearthquake sequence consisting of 13 surface-rupturing events since 60 ka.The seismic history can be divided into three periods based on different recurrence intervals.Between 60 and 40 ka,three earthquakes occurred with recurrence interval of ~10 ka.From 40 to 25 ka,there were six earthquakes with the recurrence interval of about 2.5 ka.In the last 25 ka,four earthquakes have taken place with the recurrence interval varying considerably.The recurrence interval between the last three events is ~5 ka.Smaller recurrence intervals correspond to stages of faster fault slip.The coseismic displacement of a single event is 0.8 to 2.2 m,average 1.4 m,largely equivalent to moment magnitudes 6.7-7.1.This study demonstrates the feasibility of bore-hole drilling in investigating paleoearthquakes along normal faults.It also suggests that closely spaced boreholes with continuous undisturbed cores are essential for reconstructing the complete paleoearthquake sequence. | ZHANG ShiMin WANG DanDan LIU XuDong ZHANG GuoHong ZHAO JunXiang LUO MingHui REN JunJie WANG Rui ZHANG YingLi | 2008 | Science China Earth Sciences2008，51，8： | 11 |
| 8 | Defined tumor antigen-specific T cells potentiate personalized TCR-T cell therapy and prediction of immunotherapy response显示文摘Personalized immunotherapy targeting tumor-specific antigens(TSAs)could generate efficient and safe antitumor immune response without damaging normal tissues.Although neoantigen vaccines have shown therapeutic effect in clinic trials,precise prediction of neoantigens from tumor mutations is still challenging.The host antitumor immune response selects and activates T cells recognizing tumor antigens.Hence,T cells engineered with T-cell receptors(TCRs)from these naturally occurring tumor antigen-specific T(Tas)cells in a patient will target personal TSAs in his/her tumor.To establish such a personalized TCR-T cell therapy,we comprehensively characterized T cells in tumor and its adjacent tissues by single-cell mRNA sequencing(scRNA-seq),TCR sequencing(TCR-seq)and in vitro neoantigen stimulation.Compared to bystander T cells circulating among tissues,Tas cells were characterized by tumor enrichment,tumor-specific clonal expansion and neoantigen specificity.We found that CXCL13 is a unique marker for both CD4^(+)and CD8^(+)Tas cells.Importantly,TCR-T cells expressing TCRs from Tas cells showed significant therapeutic effects on autologous patient-derived xenograft(PDX)tumors.Intratumoral Tas cell levels measured by CXCL13 expression precisely predicted the response to immune checkpoint blockade,indicating a critical role of Tas cells in the antitumor immunity.We further identified CD200 and ENTPD1 as surface markers for CD4^(^(+))and CD8^(^(+))Tas cells respectively,which enabled the isolation of Tas cells from tumor by Fluorescence Activating Cell Sorter(FACS)sorting.Overall,our results suggest that TCR-T cells engineered with Tas TCRs are a promising agent for personalized immunotherapy,and intratumoral Tas cell levels determine the response to immunotherapy. | Jingjing He Xinxin Xiong Han Yang Dandan Li Xuefei Liu Shuo Li Shuangye Liao Siyu Chen Xizhi Wen Kuai Yu Lingyi Fu Xingjun Dong Kaiyu Zhu Xiaojun Xia Tiebang Kang Chaochao Bian Xiang Li Haiping Liu Peirong Ding Xiaoshi Zhang Zhenjiang Liu Wende Li Zhixiang Zuo Penghui Zhou | 2022 | Cell Research2022，32，6： | 10 |
| 9 | A novel role of hematopoietic CCL5 in promoting triple-negative mammary tumor progression by regulating generation of myeloid-derived suppressor cells显示文摘CCL5 是响应压力信号在有免疫力、非有免疫力的房间的一个宽数组表示的 CC chemokine 家庭的一个成员。有先进人的乳癌的 CCL5 表示相互关联。然而,它行动的功能的意义和模式没被建立。这里,我们证明 CCL5 缺乏的老鼠对高度好攻击的、三元组否定的乳房的肿瘤生长抵抗。造血的 CCL5 在这显型是主导的。造血的 CCL5 的缺席由积累 Ly6C +/Gr-1+, 的异常产生导出 myeloid 的 suppressor 房间(MDSC )> 嗨有损害能力到的 和 Ly6G + MDSC 压制 CD8 + T 房间的 cytotoxicity。CCL5 的这些性质在 orthotopic 和自发的乳房的肿瘤被观察。CCL5 的调停抗体的全身的封锁禁止肿瘤前进并且对非产生免疫性的肿瘤提高治疗学的种痘的功效。CCL5 也帮助维持人的 MDSC 的抑制免疫力的能力。我们的学习揭开经由与导出肿瘤的刺激殖民地的因素在合作在骨头髓产生 MDSC 支持乳房的肿瘤前进的造血地导出的 CCL5 的一项新奇、 chemokine 独立的活动。学习在在造血的分隔空间和肿瘤壁龛之间的相互影响上打开可观的灯。因为在正常生理学的这个分子的明显的非必需的性质, CCL5 可以象有 MDSC 渗入的重要数量的大量稳固的肿瘤一样为乳癌在免疫疗法代表一个优秀治疗学的目标。 | Yan Zhang Dandan Lv Ha-Jeong Kim Robert A Kurt Wen Bu Yi Li Xiaojing Ma | 2013 | Cell Research2013，23，3： | 9 |
| 10 | Effect of supplementation of allicin on methanogenesis and ruminal microbial flora in Dorper crossbred ewes显示文摘Background: Garlic extracts have been reported to be effective in reducing methanogenesis. Related mechanisms are not well illustrated, however, and most studies have been conducted in vitro. This study investigates the effects of supplementary allicin(AL) in sheep diet on in vivo digestibility, rumen fermentation, and shifts of microbial flora.Methods: Two experiments were conducted using Dorper × thin-tailed Han crossbred ewes. In experiment 1,eighteen ewes(60.0 ± 1.73 kg BW) were randomly assigned for 29 days to either of two dietary treatments: a basal diet or the basal diet supplemented with 2.0 g AL/head?day to investigate supplementary AL on nutrient digestibility and methane emissions. In experiment 2, six ewes(65.2 ± 2.0 kg BW) with ruminal canulas were assigned to the same two dietary treatments as in experiment 1 for 42 days to investigate supplementary AL on ruminal fermentation and microbial flora. The methane emissions were determined using an open-circuit respirometry system and microbial assessment was done by q PCR of 16 S r RNA genes.Results: Supplementary AL increased the apparent digestibility of organic matter(P < 0.001), nitrogen(P = 0.006),neutral detergent fiber(P < 0.001), and acid detergent fiber(P = 0.002). Fecal nitrogen output was reduced(P = 0.001)but urinary nitrogen output was unaffected(P = 0.691), while nitrogen retention(P = 0.077) and nitrogen retention/nitrogen intake(P = 0.077) tended to increase. Supplementary AL decreased methane emissions scaled to metabolic bodyweight by 5.95 %(P = 0.007) and to digestible organic matter intake by 8.36 %(P = 0.009). Ruminal p H was unaffected(P = 0.601) while ammonia decreased(P = 0.024) and total volatile fatty acids increased(P = 0.024) in response to supplementary AL. Supplementary AL decreased the population of methanogens(P = 0.001) and tended to decrease that of protozoans(P = 0.097), but increased the populations of F. succinogenes(P < 0.001), R. flavefaciens(P = 0.001), and B. fibrisolvens(P = 0.001).Conclusions: Supplementation of AL at 2.0 g/head?day effectively enhanced OM, N, NDF, and ADF digestibility and reduced daily methane emissions(L/kg BW0.75) in ewes, probably by decreasing the population of ruminal protozoans and methanogens. | Tao Ma Dandan Chen Yan Tu Naifeng Zhang Bingwen Si Kaidong Deng Qiyu Diao | 2016 | Journal of Animal Science and Biotechnology2016，7，2： | 9 |
| 11 | Allele-aware chromosome-level genome assembly of Artemisia annua reveals the correlation between ADS expansion and artemisinin yield显示文摘Artemisia annua is the major natural source of artemisinin,an anti-malarial medicine commonly used worldwide.Here,we present chromosome-level haploid maps for two A.annua strains with different artemisinin contents to explore the relationships between genomic organization and artemisinin production.High-fidelity sequencing,optical mapping,and chromatin conformation capture sequencing were used to assemble the heterogeneous and repetitive genome and resolve the haplotypes of A.annua.Approximately 5o,ooo genes were annotated for each haplotype genome,and a triplication event that occurred approximately 58.12million years ago was examined for the first time in this species.A total of 3,903,467-5,193,414 variants(SNPs,indels,and structural variants)were identified in the 1.5-Gb genome during pairwise comparison between haplotypes,consistent with the high heterozygosity of this species.Genomic analyses revealed a correlation between artemisinin concents and the copy number of amorpha-4,11-dienes ynthasegenes.This correlation was further confirmed by resequencing of 36A.annua samples with varied artemisinin contents.Circular consensus sequencing of transcripts facilitated the detection of paralog expression.Collectively,our study provides chromosome-level allele-aware genome assemblies for two A.annua strains and new insights into the biosynthesis of artemisinin and its regulation,which will contribute to conquering malaria worldwide. | Baosheng Liao Xiaofeng Shen Li Xiang Shuai Guo Shiyu Chen Ying Meng Yu Liang Dandan Ding Junqi Bai Dong Zhang Tomasz Czechowski Yi Li Hui Yao Tingyu Mai Caroline Howard Chao Sun Haitao Liu Jiushi Liu Jin Pei Jihai Gao Jigang Wang Xiaohui Qiu Zhihai Huang Hongyi Li Ling Yuan Jianhe Wei lan Graham Jiang Xu Boli Zhang Shilin Chen | 2022 | Molecular Plant2022，15，8： | 9 |
| 12 | Tom70 serves as a molecular switch to determine pathological cardiac hypertrophy显示文摘病理学的心脏的肥大是心失败的不可避免的 forerunner。不管心脏的肥大的病原学, cardiomyocyte mitochondrial 改变总是在这上下文被观察。mitochondrial 先锋蛋白质的进口调节在 pathophysiological 下面维持 mitochondrial 功能的外部膜(汤姆) 建筑群管理的 mitochondrial 的 translocases;然而,它在病理学的心脏的肥大的发展的角色仍然保持不清楚。这里,我们证明 Tom70 是在从人和试验性的动物的病理学的 hypertrophic 心的 downregulated。在 Tom70 表示的减小在 vivo 并且在 vitro 生产了不同病理学的 cardiomyocyte 肥大。指向 Tom70 的眼的 atrophy-1 的有缺点的 mitochondrial 进口触发了细胞内部的氧化应力,它导致了病理学的细胞的回答。重要地,如果有到多样的 pro-hypertrophic 的完整的抵抗的 cardiomyocytes 侮辱,增加了 Tom70 层次。一起,这些结果表明 Tom70 充当安排 hypertrophic 压力和 mitochondrial 回答决定病理学的心脏的肥大的一个分子的开关。 | Jun Li Man Qi Changming Li Dan Shi Dasheng Zhang Duanyang Xie Tianyou Yuan Jing Feng Yi Liu Dandan Liang Xinran Xu Jinjin Chen Liang Xu Hong Zhang Jiangchuan Ye Fei LV Jian Huang Luying Peng Yi-Han Chen | 2014 | Cell Research2014，24，8： | 9 |
| 13 | Primarily screening and analyzing ESTs differentially expressed in rats' primary liver cancer显示文摘Objective: To screen and analyze key express sequence tags (ESTs) which were differentially displayed in every period of SD rats' primary hepatic carcinoma and reveal the molecular mechanism of carcinogenesis. Methods: Using diethylnitrosamine (DENA) as a cancerigenic agent, animal models with different phases of primary hepatic cancer were constructed in SD rats. Rats were respectively sacrificed at d 14, d 28, d 56, d 77, d 105 and d 112 after the rats received DENA by gavage, then the livers were harvested. One part of the livers was classified according to their pathological changes, while the other was reserved for molecular mechanism studies on hepatocarcinogenesis. The differentially expressed genes were isolated from both normal and morbid tissues by mRNA differential display technique (DDRT-PCR). After the fragments were sequenced, bioinformatics were used to analyze the results. Results: Twelve differentially expressed cDNA fragments were obtained. Nine fragments had the homology with known cDNA clones, especially EST-7 was similar to BN/SsNHsdMCW mitochondrion gene and the identity was 100% which suggested EST-7 may be the part of BN/SsNHsdMCW mitochondrion gene. In contrast, other three fragments (EST-1, EST-3 and EST-5) had extremely low identity to any genes registered in GENBANK databases. Conclusions: BN/SsNHsdMCW mitochondrion gene was expressed in different periods of hepatocarcinogenesis. Moreover, EST-1, EST-3 and EST-5 were suggested to contribute to the development of rat hepatocarcinogenesis, and thus may be candidates of new targets of oncogenes or cancer suppressor genes. | Dandan Liu Lijuan Zhi Mingxia Ma Dan Qiao Meijuan Wang Yawei Wang Baijie Jin Anqi Li Guting Liu Yiqing Zhang Yanyan Song Hongxu Zhang | 2013 | Chinese Journal of Cancer Research2013，25，1： | 9 |
| 14 | CD4^(+)CD25^(+)but not CD4^(+)Foxp3^(+) T cells as a regulatory subset in primary biliary cirrhosis显示文摘Increasing evidence indicates a role for regulatory T cells(Tregs)in the immune response and in autoimmune diseases,but the role of Tregs and cytokines in autoimmune hepatic diseases remains largely unclear and controversial,especially in patients with primary biliary cirrhosis(PBC).This study was undertaken to investigate Tregs and different cytokines in the liver and peripheral blood of PBC patients.We found that these patients demonstrated a reduction of CD4^(+)CD25^(+) T cells but elevated CD4^(+)Foxp3^(+) T cells in peripheral blood mononuclear cells(PBMCs)and CD41 T cells.The percentage of CD4^(+)CD25^(+) T cells in PBMCs was negatively correlated with elevated plasma interferon(IFN)-c levels.A liver-specific analysis showed that the frequency of Foxp31 Tregs,transforming growth factor(TGF)-b1 and IFN-c were increased in PBC patients.Our findings suggest that an imbalance between CD4^(+)CD25^(+) Tregs and cytotoxic cytokines plays a crucial role in the pathogenesis of PBC while the role of Foxp3 needs further investigation. | Dandan Wang Huayong Zhang Jun Liang Zhifeng Gu Qiang Zhou Xiangshan Fan Yayi Hou Lingyun Sun | 2010 | Cellular & Molecular Immunology2010，7，6： | 9 |
| 15 | High-Performance Li-ion Batteries and Super-capacitors Based on Prospective 1-D Nanomaterials显示文摘One-dimensional(1-D) nanomaterials with superior specific capacity, higher rate capability, better cycling peroperties have demonstrated significant advantages for high-performance Li-ion batteries and supercapacitors. This review describes some recent developments on the rechargeable electrodes by using 1-D nanomaterials(such as Li Mn2O4 nanowires, carbon nanofibers, Ni Mo O4 · n H2O nanorods, V2O5 nanoribbons,carbon nanotubes, etc.). New preparation methods and superior electrochemical properties of the 1-D nanomaterials including carbon nanotube(CNT), some oxides, transition metal compounds and polymers, and their composites are emphatically introduced. The VGCF/Li Fe PO4/C triaxial nanowire cathodes for Li-ion battery present a positive cycling performance without any degradation in almost theoretical capacity(160 m Ah/g).The Si nanowire anodes for Li-ion battery show the highest known theoretical charge capacity(4277 m Ah/g),that is about 11 times lager than that of the commercial graphite(372 m Ah/g). The SWCNT/Ni foam electrodes for supercapacitor display small equivalent series resistance(ESR, 52 m?) and impressive high power density(20 k W/kg). The advantages and challenges associated with the application of these materials for energy conversion and storage devices are highlighted. | Dandan Zhao Ying Wang Yafei Zhang | 2011 | Nano-Micro Letters2011，3，1： | 9 |
| 16 | miR164c and miR168a regulate seed vigor in rice^∞显示文摘Micro RNAs(miRNAs)are key regulators of gene expression in many important biological processes of plants.However,few miRNAs have been shown to regulate seed vigor.Here,we conducted microarray assays to analyze miRNA expression levels in seeds of the rice(Oryza sativa L.)cultivar ZR02.Results showed significant differences in the expression of 11 miRNAs between artificially aged and untreated control seeds.Among these,osa-miR164c was transcriptionally upregulated,while osamiR168 a was downregulated in artificially aged seeds;this was verified by quantitative real-time PCR analysis.Under the same aging condition,osa-miR164c overexpression in OE164c transgenic seeds and osa-miR168a silencing in MIM168a transgenic seeds of the rice cultivar Kasalath led to lower germination rates,whereas osa-miR164c silencing Rin MIM164c and osa-miR168a overexpression in OE168a resulted in higher seed germination rates compared with wild-type seeds.Meanwhile,changes in cytomembrane permeability of seeds and in the expression level of osa-miR164c target genes(OsPM27 and OsPSK5)and osamiR168a target genes(OsAGO1 and OsPTR2)under aging conditions coincided with changes in seed vigor induced by osa-miR164c and osa-miR168 a.Thus,genetic manipulation of miRNAs has important implications in the development of crop cultivars with high vigor and extended life span of seeds. | Yan Zhou Shiqi Zhou Liping Wang Duo Wu Hailan Cheng Xu Du Dandan Mao Chunlai Zhang Xiaocheng Jiang | 2020 | Journal of Integrative Plant Biology2020，62，4： | 7 |
| 17 | Characterization of flavor fingerprinting of red sufu during fermentation and the comparison of volatiles of typical products显示文摘Traditional Chinese red sufu is a popular condiment,and typical flavor is an important indicator of sensory qualities in commercial products.In this study,the volatile aroma analysis of Wangzhihe red sufu(WRS)from different fermentation stages and four typical red sufu products from different locations was carried out by gas chromatography-mass spectrometry(GC–MS),gas chromatography-mass spectrometry/olfactory(GC–MS/O),electronic nose(E-nose)and sensory evaluation.Results showed that 106 volatile compounds were identified in Wangzhihe red sufu during fermentation process,in which phenolics and alcohols were dominant at molded and salted phetze stages,while esters and alcohols became predominate at post-fermentation stage.The volatile aroma substances varied at each fermentation stage.Furthermore,86 volatiles,including 16 aroma-active compounds,were detected in four typical red sufu products.The multivariate analysis results showed the difference in samples from different fermentation stages and typical red sufu products according to GC–MS and E-nose analysis.Combined with flavor omics,discriminant model was established for effective discrimination of samples from different fermentation stages and locations,as well as references to sufu maturity extent.The study presented a new strategy for quality evaluation of red sufu,which could be the supplement to quality evaluation standards. | Peng Wang Xiaoran Ma Wenping Wang Dandan Xu Xin Zhang Jian Zhang Yong Sun | 2019 | Food Science and Human Wellness2019，8，4： | 7 |
| 18 | :CL5 as a potential immunotherapeutic target in triple-negative breast cancer显示文摘乳癌(BC ) 是在在世界上的女人之中的死亡的一个领先的原因。迄今为止,很多个分子作为疾病地位指示物和治疗学的目标被建立了。在他们之中知道的最好是雌激素 receptor-α(ER-α) ,孕酮受体(PR ) 和 HER-2/neu。大约 15 % -20%BC 病人不对指向支持肿瘤的因素的这些班的治疗有效地作出回应。因此,另外的目标强烈并且着急地被寻求在以后在为为否定的人的英国计算机学会的治疗嗯, PR 和 HER-2,所谓的三元组否定的 BC (TNBC ) 。最近的临床的工作揭示了那 CC chemokine ligand (CCL5 ) 5 强烈与 BC 的前进被联系,特别地 TNBC。CCL5 怎么贡献 TNBC 的发展,很好没被理解。试验性的动物研究开始处理了机械学的问题。在这篇文章,我们将考察临床并且在导致了我们在 TNBC 的那指向的 CCL5 将有的自己的假设的这个区域的实验室工作有一般生理学上的最小的不利影响的有利治疗学的结果。 | Dandan Lv Yan Zhang Ha-Jeong Kim Lixing Zhang Xiaojing Ma | 2013 | Cellular & Molecular Immunology2013，10，4： | 7 |
| 19 | Aneuploidy of chromosome 8 in circulating tumor cells correlates with prognosis in patients with advanced gastric cancer显示文摘Objective: Previous work indicated that aneuploidy of chromosome 8 in circulating tumor cells(CTCs)correlated with therapeutic efficacy for advanced gastric cancer(AGC) patients. In this follow-up study performed on the same population of AGC patients, we investigated whether and how aneuploidy of chromosome 8 in CTCs correlates with patients' clinical prognosis.Methods: The prospective study was performed on 31 patients with newly diagnosed AGC. Previously established integrated subtraction enrichment(SE) and immunostaining-fluorescence in situ hybridization(i FISH)platform was applied to identify, enumerate and characterize CTCs. Quantification of CTCs and analysis of their aneuploidy of chromosome 8 were performed on patients before and after therapy.Results: CTCs were measured in 93.5% of AGC patients, and two CTC subtypes with diverse threshold values were identified, multiploid CTCs with the threshold of ≥2 per 7.5 m L and multiploid plus triploid CTCs with the threshold of ≥4, which were found to significantly correlate with poor progression-free survival(PFS) and overall survival(OS). In particular, patients with ≥10% increased multiploid CTCs after an initial 6 weeks of therapy had poor PFS and OS, whereas improved PFS and OS were observed on those who had ≥10% decreased multiploid CTCs. After adjusting for clinically significant factors, ≥10% increased post-therapy multiploid CTCs was the only independent predictor of PFS and OS.Conclusions: Aneuploidy of CTCs correlates with prognosis of AGC patients. Quantitative comparison monitoring multiploid CTCs before and after therapy may help predict improved or inferior prognosis and chemoresistance. | Yilin Li Xiaotian Zhang Jifang Gong Qiyue Zhang Jing Gao Yanshuo Cao Daisy Dandan Wang Peter Ping Lin Lin Shen | 2016 | Chinese Journal of Cancer Research2016，28，6： | 7 |
| 20 | Induction of OTUD4 by viral infection promotes antiviral responses through deubiquitinating and stabilizing MAVS显示文摘The activity and stability of the adapter protein MAVS (also known as VISA, Cardif and IPS-1), which critically mediates cellular antiviral responses, are extensively regulated by ubiquitination. However, the process whereby MAVS is deubiquitinated is unclear. Here, we report that the ovarian tumor family deubiquitinase 4 (OTUD4) targets MAVS for deubiquitination. Viral infection leads to the IRF3/7-dependent upregulation of OTUD4 which interacts with MAVS to remove K48-linked polyubiquitin chains, thereby maintaining MAVS stability and promoting innate antiviral signaling. Knockout or knockdown of OTUD4 impairs RNA virus-triggered activation of IRF3 and NF-κB, expression of their downstream target genes, and potentiates VSV replication in vitro and in vivo. Consistently, Cre-ER Otud4fl/fl or Lyz2-Cre Otud4fl/fl mice produce decreased levels of type I interferons and proinflammatory cytokines and exhibit increased sensitivity to VSV infection compared to their control littermates. In addition, reconstitution of MAVS into OTUD4-deficient cells restores virus-induced expression of downstream genes and cellular antiviral responses. Together, our findings uncover an essential role of OTUD4 in virus-triggered signaling and contribute to the understanding of deubiquitination-mediated regulation of innate antiviral responses. | Tianzi Liuyu Keying Yu Liya Ye Zhidong Zhang Man Zhang Yujie Ren Zeng Cai Qiyun Zhu Dandan Lin Bo Zhong | 2019 | Cell Research2019，29，1： | 7 |
您所在的机构暂未开通此部分的功能，请联系管理员