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| 1 | Monoamine alterations and rotational asymmetry in a rat model of Parkinson's disease following lateral ventricle transplantation of human amniotic epithelial cells显示文摘BACKGROUND: Human amniotic epithelial cells (HAECs) can differentiate into neurons, astrocytes,and oligodendrocytes. They biologically secrete many active neurotrophins and have the capacity tometabolize dopamine enzymes. These features underlie a theoretical basis for the treatment ofParkinson's disease (PD).OBJECTIVE: To investigate the survival and differentiation of transplanted HAECs in the lateralventricle of PD model rats, and to explore its effect on circling behavior, as well as levels ofdopamine (DA), the metabolite homovanillic acid, dihydroxyphenyl acetic acid,5-hydroxyindoleacetic acid, and 5-hydroxytryptamine in the striatum.DESIGN, TIME AND SETTING: A randomized, controlled, animal study was performed at theInstitute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, ChineseAcademy of Sciences, and Shanghai Celstar Institute of Biotechnology from May 2007 to December2008.MATERIALS: HAECs were derived from the placental chorion following caesarean delivery at theShanghai International Maternal and Child Health Hospital. 6-hydroxydopamine (6-OHDA), andmouse anti-human Vimentin monoclonal antibody were purchased from Sigma, USA; mouseanti-human nestin and tyrosine hydroxylase (TH) monoclonal antibodies were purchased fromChemicon, USA.METHODS: A total of 114 healthy, adult, Sprague Dawley rats were randomly assigned to twogroups: PD model [n = 90, stereotactic microinjection of 2 μL 6-OHDA (3.5 μg/μL) into the striatum]and control (n = 24, no treatment). The 51 successful PD model rats were randomly divided into 3subgroups (n = 17): HAEC, PBS, and model. The HAEC and PBS groups were respectively injectedwith 10 μL PBS solution containing 1 x 105/mL HAECs or 10 μL PBS into the lateral ventricle. Themodel group was not treated.MAIN OUTCOME MEASURES: TH protein expression in the striatum was evaluated byimmunohistochemistry 5 weeks after HAEC transplantation. At 10 weeks, HAEC survival in thelateral ventricle was investigated by immunofluorescent staining; differentiation of HAECs in thelateral and third ventricles was examined by TH immunohistochemistry; concentrations of DA,homovanillic acid, dihydroxyphenyl acetic acid, 5-hydroxyindoleacetic acid, and 5-hydroxytryptaminein the striatum, as well as DA concentration in the cerebrospinal fluid, were measured withhigh-performance liquid chromatography-electrochemical detection. Circling behavior of PD modelrats was consecutively observed for 10 weeks following intraperitoneal injection of amphetamine 1week after successful model establishment.RESULTS: In the HAEC group, the number of TH-positive cells significantly increased in thestriatum, and circling behavior significantly decreased, compared with the PBS and model groups(P < 0.01). In addition, monoamine concentrations in the striatum, as well as DA concentrations inthe cerebrospinal fluid, significantly increased, compared with the PBS group (P < 0.05-0.01 ).Moreover, a large number of nestin-, vimentin-, and TH-positive cells were observed in the lateraland third ventricles following HAEC injection. CONCLUSION: HAECs survived for 10 weeks with no overgrowth following transplantation into thelateral ventricle of PD model rats. Moreover, the cells differentiated into dopaminergic neurons,which increased DA secretion. HAEC transplantation improved cycling behavior in PD model rats. | Shouru Xue Xinxin Yang Wanli Dong Guozhen Hui Lihe GUO | 2009 | Neural Regeneration Research2009，4，12： | 6 |
| 2 | Groundwater Systems and Resources in the Ordos Basin,China显示文摘Ordos 盆是在西北的中国的大规模沉积的盆。到顶的从底部的 hydrostratigraphic 单位是 pre 寒武纪的变形岩石,更低的古生代的碳酸盐岩石,上面对中生代碎屑状的岩石和新生代存款古生代。全部的厚度直到 6000 m。三个地下水系统在 Ordos 盆是在场的,基于地质的设置,即石灰岩地区常见的地形地下水系统,白垩纪碎屑状的地下水系统和第四级的地下水系统。这份报纸系统地描述每个系统的地下水流动模式和对地下水资源的全面评价。 | HOU Guangcai LIANG Yongping SU Xiaosi ZHAO Zhenghong TAO Zhengping YIN Lihe YANG Yuncheng WANG Xiaoyong | 2008 | Acta Geologica Sinica(English Edition)2008，82，5： | 5 |
| 3 | SEMA3A-mediated crosstalk between prostate cancer cells and tumor-associated macrophages promotes androgen deprivation therapy resistance显示文摘Androgen deprivation therapy(ADT)is an important treatment strategy that can be used to delay the progression of prostate cancer.However,after an initial response,which varies significantly among patients,the disease eventually progresses despite the low levels of testosterone in the systemic circulation.1 The mechanism underlying the progression of prostate cancer to castration-resistant prostate cancer(CRPC)is not yet clear,and further investigation is needed. | Fei Liu Chao Wang Hai Huang Yue Yang Lihe Dai Sujun Han Nianzeng Xing Shancheng Ren | 2021 | Cellular & Molecular Immunology2021，18，3： | 5 |
| 4 | Synthesis and Anti-HIV Activity of a Series of 6-Modified 2',3'-Dideoxyguanosine and 2',3'-Didehydro-2',3'- dideoxyguanosine Analogs显示文摘寻找潜在的 2,3-dideoxyguanosine (ddG ) 和 2,3-didehydro-2,3-dideoxyguanosine (D4G ) prodrugs,一系列 6-modified ddG, D4G 类似物在基于房间的试金为他们的 anti-HIV 活动和 cytotoxities 被综合并且评估。所有类似物显示出低 cytotoxicities,他们中的一些显示了良性的 anti-HIV 活动。在 vivo, ddGTP 和 D4TTP 的活跃 triphosphate 形式,被一个新奇、灵巧的“一个壶”也综合方法。由 Taq, Therminater DNA 聚合酶和在 DNA/RNA 海滨合并的 HIV 反向的 transcriptase (RT ) 的 ddGTP 和 D4TTP 的识别被一个非放射性方法调查, Km 是坚定的。 | Lujia Xie Xiantao Yang Delin Pan Yingli Cao Mou Cao Guichun Lin Zhu Guan Ying Guo Lihe Zhang Zhenjun Yang | 2013 | Chinese Journal of Chemistry2013，31，9： | 2 |
| 5 | The novel amyloid-beta peptide aptamer inhibits intracellular amyloid-beta peptide toxicity显示文摘Amyloid β peptide binding alcohol dehydrogenase (ABAD) decoy peptide (DP) can competitively antagonize binding of amyloid β peptide to ABAD and inhibit the cytotoxic effects of amyloid β peptide. Based on peptide aptamers, the present study inserted ABAD-DP into the disulfide bond of human thioredoxin (TRX) using molecular cloning technique to construct a fusion gene that can express the TRX1-ABAD-DP-TRX2 aptamer. Moreover, adeno-associated virus was used to allow its stable expression. Immunofluorescent staining revealed the co-expression of the transduced fusion gene TRX1-ABAD-DP-TRX2 and amyloid β peptide in NIH-3T3 cells, indicating that the TRX1-ABAD-DP-TRX2 aptamer can bind amyloid β peptide within cells. In addition, cell morphology and MTT results suggested that TRX1-ABAD-DP-TRX2 attenuated amyloid β peptide-induced SH-SY5Y cell injury and improved cell viability. These findings confirmed the possibility of constructing TRX-based peptide aptamer using ABAD-DP. Moreover, TRX1-ABAD-DP-TRX2 inhibited the cytotoxic effect of amyloid β peptide. | Xu Wang Yi Yang Mingyue Jia Chi Ma Mingyu Wang Lihe Che Yu Yang Jiang Wu | 2013 | Neural Regeneration Research2013，8，1： | 2 |
| 6 | Effects of biological activity, light, temperature and oxygen on phosphorus release processes at the sediment and water interface of Taihu Lake, China显示文摘 | Xia Jiang Xiangcan Jin Yang Yao Lihe Li Fengchang Wu | 2008 | Water Research2008，，8： | 2 |
| 7 | Growth and optical properties of ytterbium and rare earth ions codoped CaF2-SrF2 eutectic solid-solution(RE=Y^(3+),Gd^(3+),La^(3+))显示文摘Ytterbium and rare earth ions(RE=Y,Gd,La)codoped CaF_(2)-SrF_(2)single crystals(3 at%Yb,6 at%RE:CaF_(2)-SrF_(2))were fabricated by temperature gradient technology(TGT).All the space groups remain the same Fm3m as that of Yb:CaF_(2)-SrF_(2).The lattice parameter a,unit cell volume V,as well as bond length of Ca/Sr-F and F-F increase in the sequence of rare-earth ions radius Y^(3+) | Binbin Jiang Lihe Zheng Dapeng Jiang Handi Yin Jiangang Zheng Qiuhong Yang Guofeng Cheng Liangbi Su | 2021 | Journal of Rare Earths2021，39，4： | 2 |
| 8 | Graph-regularized saliency detection with convex-hull-based center prior显示文摘 | Chuan Yang Lihe Zhang Huchuan Lu | 2013 | IEEE Sigal Processing Letters2013，20，7： | 1 |
| 9 | Test on evaluation method for biodegradability of lubricants显示文摘 | Yang Lihe Zhu Yong Fan Guochen | 2008 | Transactions of Tianjin University2008，14，： | 1 |
| 10 | 显示文摘 | Yang Lihe Dong Weifu Shi Dongjian | 2011 | Journal of Functional Materials2011，42，3： | 1 |
| 11 | A new approach to measure melamine, cyanuric acid, and melamine cyanurate using surface enhanced Raman spectroscopy coupled with gold nanosubstrates 显示文摘 | Li Lihe Yang Liu Meng Shilin | 2008 | Food Qna12008，，2： | 1 |
| 12 | Nucleolin-targeting liposomes guided by aptamer AS1411 for the delivery of siRNA for the treatment of malignant melanomas显示文摘 | Liyu Li Jianjun Hou Xinjie Liu Yujia Guo Yun Wu Lihe Zhang Zhenjun Yang | 2014 | Biomaterials2014，，： | 1 |
| 13 | Loss of silencing activity caused by 5′-terminal modification with D-/L-isonucleotide(isoNA) or locked nucleic acid(LNA) could not be restored by 5′-terminal phosphorylation显示文摘In this study,a series of 5′-phosphorylated siRNAs with D-/L-isonucleotide(isoNA)or locked nucleic acid(LNA)incorporated at the 5′-terminus were synthesized.It was found that after incorporating either isoNA or LNA at the 5′-terminus of the antisense strand or sense strand,the silencing activity of modified strands has been inhibited,which cannot be recovered by phosphorylation at the 5′-terminus;however,the silencing activity of unmodified strand to its own target was increased.This work indicates that the isoNA and LNA modification at 5′-terminus can interfere with the strand selection during the RISC assembling process,and the disturbance of the 5′-phosporylation should not be the only viable mechanism. | HUANG Ye CHEN Zhuo WANG Zhuo LI YaTing CHEN Yue YANG ZhenJun ZHANG LiHe | 2014 | Science China Chemistry2014，57，2： | 1 |
| 14 | Unfolding and Conformational Variations of Thrombin‐Binding DNA Aptamers: Synthesis, Circular Dichroism and Molecular Dynamics Simulations显示文摘 | Lidan Sun Hongwei Jin Xiaoyang Zhao Zhenming Liu Yifu Guan Zhenjun Yang Liangren Zhang Lihe Zhang | 2014 | ChemMedChem2014，，5： | 1 |
| 15 | Transparent System for Digital Media Copyright Protection in Mobile Business显示文摘At present, in mobile business, the secure environment in the terminal of users has not been embedded, many cryptology-based methods can not be directly used to protect the copyright of digital media. Under this situation, a transparent system based on watermark for digital right management and digital copyright protection is proposed in this paper. The transparent system is called WDRM (watermark-based digital rights management) and transparent to users. Its core is WDRM Agent. In this paper, system inner model and procession are designed in detail at first, including the registration, the downloading, the super distribution, and the cooperation. Because the watermark embedding and the extraction algorithms are the kernel in this architecture, we also discuss how to choose and design the algorithms. Then we establish a package for all algorithms in WDRM Agent. | YANG Cheng ZHANG Lihe WANG Yongbin LIU Jianbo | 2006 | Wuhan University Journal of Natural Sciences2006，11，6： | 1 |
| 16 | In vitro selection of G-rich RNA aptamers that target HIV-1 integrase显示文摘Aptamers that interact with various HIV-1 proteins,such as reverse transcriptase,Rev,Tat protein,and nuclear capsule protein,have been prepared through SELEX (systematic evolution of ligands by ex-ponential enrichment) technique. However,there are few reports about the DNA or RNA aptamers that target HIV-1 integrase. In this investigation,we selected alternative RNA aptamers specific for the HIV-1 integrase by using a different binding buffer containing 10 mmol·L-1 MgCl2 and 100 mmol·L-1 KCl. Aptamer IN1,IN2,IN3 had similar and the highest Kd values from 145 to 239 nmol·L-1. Structural studies showed that they formed similar stem-loop structure. Deletion of any stem structure resulted in diminished affinity. In addition,structure probing study with antisense DNA indicated that the stem-loop structure in the random region was critical for integrase binding. Although aptamer IN1 failed to form G-quartet structure,it might directly interact with the DDE motif of integrase,which is the virus DNA-binding site,because G-quadruplex T40214 competitively inhibited the interaction between IN1 and integrase. Together,this study generated a novel RNA aptamer IN1,which could be useful in basic research and anti-HIV drug screening. | LIU YingChun ZHANG Yan YE GuoZhu YANG ZhenJun ZHANG LiangRen ZHANG LiHe | 2008 | Science China Chemistry2008，51，5： | 0 |
| 17 | NMR Study on Fluoroisonucleosides( I)——Determination of the Configuration and Conformation of 1' -a-Methoxy-2' -Deoxy-2' -B-Ruoro-2' -a-Uracil -3' ,4' -o-lsopropylidene-L-Arabinoside显示文摘The present paper reports the determination of the configuration and Conformation of 1’ -a-methoxy-2’ -deoxy-2’-β-fluoro-2’-α-uracil-3’,4’ -o-isopropylidene-L-arabinoside obtained from silylated uracil by using 13C-NMR and 2D-NMR. | Gao Congyuan and Qiao Hang (Center of Analysis & Computation, Beijing Medical University, Beijing)Ma Lingtai, Yang Xianbin, Dong Lingjiao and Zhang Lihe (School of Pharmaceutical Science, Beijing Medical University, Beijing) | 1990 | Chemical Research in Chinese Universities1990，6，3： | 0 |
| 18 | Visual-feature-assisted mobile robot localization in a long corridor environment显示文摘Localization plays a vital role in the mobile robot navigation system and is a fundamental capability for autonomous movement.In an indoor environment,the current mainstream localization scheme uses two-dimensional(2D)laser light detection and ranging(LiDAR)to build an occupancy grid map with simultaneous localization and mapping(SLAM)technology;it then locates the robot based on the known grid map.However,such solutions work effectively only in those areas with salient geometrical features.For areas with repeated,symmetrical,or similar structures,such as a long corridor,the conventional particle filtering method will fail.To solve this crucial problem,this paper presents a novel coarse-to-fine paradigm that uses visual features to assist mobile robot localization in a long corridor.First,the mobile robot is remote-controlled to move from the starting position to the end along a middle line.In the moving process,a grid map is built using the laser-based SLAM method.At the same time,a visual map consisting of special images which are keyframes is created according to a keyframe selection strategy.The keyframes are associated with the robot’s poses through timestamps.Second,a moving strategy is proposed,based on the extracted range features of the laser scans,to decide on an initial rough position.This is vital for the mobile robot because it gives instructions on where the robot needs to move to adjust its pose.Third,the mobile robot captures images in a proper perspective according to the moving strategy and matches them with the image map to achieve a coarse localization.Finally,an improved particle filtering method is presented to achieve fine localization.Experimental results show that our method is effective and robust for global localization.The localization success rate reaches 98.8%while the average moving distance is only 0.31 m.In addition,the method works well when the mobile robot is kidnapped to another position in the corridor. | Gengyu GE Yi ZHANG Wei WANG Lihe HU Yang WANG Qin JIANG | 2023 | Frontiers of Information Technology & Electronic Engineering2023，24，6： | 0 |
| 19 | Tribological behavior of shape memory cyanate ester materials and their tunable friction mechanism显示文摘High-performance polymer friction materials with tunable tribological behavior to fit varied work conditions remain a challenge of widespread interest for a variety of applications.Shape memory polymer exhibits morphing and modulus changing over temperature changing provides a promising material to adjust the friction process.Herein,we investigated the tribological properties of shape memory cyanate ester(SMCE)under different conditions.The SMCE exhibits the tribological behavior of good friction material with stable high coefficient of friction(COF)and a low wear rate.Besides,the COF increases and wear rate decreases with the temperature increasing show the tunable friction property of the SMCE.We propose a new model of wear-compensation through shape recovery to explain the adjustable friction behavior of thermal-responsive polymer from the aspect of shape recovery and energy conversion.This study provides a high-performance friction material and paves the route for the application of shape memory polymer(SMP)in tribology field with tunable property. | Zhangzhang TANG Lihe GUO Mingkun XU Hongwei RUAN Jing YANG Tingmei WANG Jianqiang ZHANG Qihua WANG Yaoming ZHANG | 2023 | Friction2023，11，10： | 0 |
| 20 | Synthesis and biological evaluation of peptide-siRNA conjugates with phosphodiester unit as linker显示文摘In this paper,a series of peptide-siRNA conjugates with phosphodiester unit as the linker targeting to Cdc2 gene were synthesized by solid phase stepwise strategy.The conjugation of peptides at either 3’-terminus of siCdc2 bring no change to the classical A-form of RNA duplex,but slightly compromise the thermodynamic stability.Peptide conjugation at the 3’-terminus of sense strand could improve the serum stability obviously,however,the opposite peptide conjugation at the 3’-terminus of antisense strand shows no such influence.According to the results of artificial silencing activity assay system,peptide conjugation at 3’-terminus of antisense strand slightly weakens the silencing activity of siCdc2.But sense strand peptide conjugation exhibits similar silencing activity as native siCdc2,meanwhile,it could mitigate the unwanted off-target effect of sense strand targeting to its own mRNA. | WANG XiaoFeng HUANG Ye LIU Yang CHEN Yue JIN HongWei ZHENG Yi DU Quan YANG ZhenJun ZHANG LiHe | 2013 | Science China Chemistry2013，56，11： | 0 |
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