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| 1 | An Effective Method for Total RNA Isolation from Bamboo显示文摘Trizol reagent was used for RNA isolation from fresh leaves of Dendrocalamopsis oldhami, Bambusa ventricosa and Phyllostachys aureosulcata cv. Pekinensis. The extracted RNA from leaves had the normal ultraviolet absorption, the value of OD260/OD280 varied between 1.8-2.0. The 28s rRNA was more than two times brighter than 18s rRNA in electrophoresis. These results indicated that the total RNA was complete and not degraded. According to our experiments, RNA could be obtained simply with this method, and can be used for molecular manipulation such as cDNA synthesis and gene cloning. | GAO Zhimin LI Xueping LI Lubin PENG Zhenhua | 2006 | Chinese Forestry Science and Technology2006，5，3： | 39 |
| 2 | Microstructure and strengthening mechanism of hot-extruded ultralight Mg-Li-Al-Sn alloys with high strength显示文摘Duplex-structured Mg-7Li-2Al-1.5Sn alloys with high strength were fabricated and their strengthening mechanism was investigated.The Mg-7Li-2Al-1.5Sn alloys were prepared by casting and extruded at the temperature of 533 K with an extrusion ratio of 25:1.The microstructure and mechanical properties of Mg-7Li-2Al-1.5Sn alloys were systematically investigated by OM,XRD,SEM,TEM,and tensile tests.The results show that Mg-7Li-2Al-1.5Sn alloys are mainly composed ofα-Mg,β-Li,LiMgAl_(2),Mg_(2)Sn and Li_(2)MgSn phases.The yield strength(YS),ultimate tensile strength(UTS)and elongation(EL)of the extruded alloy at room temperature reach 250 MPa,324 MPa and 11.9%,respectively.A lot of Sn-rich precipitates(Mg_(2)Sn and Li_(2)MgSn)are precipitated during extrusion with an average size of∼14 nm,which is beneficial to the grain refinement.Dynamic recrystallization occurs during hot deformation and the nanoprecipitates effectively refine the dynamic recrystallized(DRXed)grains.Besides,the residual dislocations existed in DRXed and un-DRXed grains result in the dislocation strengthening in the extruded alloy.Mg7Li-2Al-1.5Sn alloys possess excellent high-temperature mechanical properties with the YS,UTS and EL of 200 MPa,237 MPa and 26.7%at 423 K,respectively.Sn-rich precipitates with good thermal stability can effectively prevent grain growth,which is good for the improvement of the high-temperature performance of Mg-Li-Al-Sn alloy. | Gang Zhou Yan Yang Hanzhu Zhang Faping Hu Xueping Zhang Chen Wen Weidong Xie Bin Jiang Xiaodong Peng Fusheng Pan | 2022 | Journal of Materials Science & Technology2022，，8： | 7 |
| 3 | Rapid Detection of Wheat Blast Pathogen Magnaporthe oryzae Triticum Pathotype Using Genome-Specific Primers and Cas12a-mediated Technology显示文摘Wheat blast,caused by the fungus Magnaporthe oryzae Triticum(MoT)pathotype,is a devastating disease persistent in South America and Bangladesh.Since MoT generally fails to cause visual symptoms in wheat until the heading stage when the infection would have advanced,disease control by fungicide application solely based on the detection of visual symptoms is ineffective.To develop an accurate and sensitive method to detect MoT at the seedling and vegetative stages for disease control,we sequenced the genomes of two MoT isolates from Brazil and identified two DNA fragments,MoT-6098 and MoT-6099,that are present in the MoT genome but not in the genome of the rice-infecting Magnaporthe oryzae Oryzae(MoO)pathotype.Using polymerase chain reaction(PCR),we confirmed the specificity of the two markers in 53 MoT and MoO isolates from South America and Bangladesh.To test the efficiency of the two markers,we first established a loop-mediated isothermal amplification(LAMP)method to detect MoT at isothermal conditions,without the use of a PCR machine.Following this,we used the Cas12a protein and guide RNAs(gRNAs)to target the MoT-6098 and MoT-6099 sequences.The activated Cas12a showed indiscriminate single-stranded deoxyribonuclease(ssDNase)activity.We then combined targetdependent Cas12a ssDNase activation with recombinase polymerase amplification(RPA)and nucleic acid lateral flow immunoassay(NALFIA)to develop a method that accurately,sensitively,and cost-effectively detects MoT-specific DNA sequences in infected wheat plants.This novel technique can be easily adapted for the rapid detection of wheat blast and other important plant diseases in the field. | Houxiang Kang Ye Peng Kangyu Hua Yufei Deng Maria Bellizzi Dipali Rani Gupta Nur Uddin Mahmud Alfredo S.Urashima Sanjoy Kumar Paul Gary Peterson Yilin Zhou Xueping Zhou Md Tofazzal Islam Guo-Liang Wang | 2021 | Engineering2021，7，9： | 4 |
| 4 | Effects of thyme(Thymus vulgaris L.)addition on the volatile compounds of mutton broth during boiling显示文摘To investigate the effects of thyme(Thymus vulgaris L.)addition on the flavor modification of mutton broth during boiling,three mutton-broth samples with various thyme contents 0.06%(S1),0.30%(S2),and1.50%(S3)were analyzed and compared,without thyme as control(0.00%,S0).The meaty,fatty,pastoral flavor and spicy were chosen as sensory attributes to evaluate the flavor of the mutton-broth samples.Sensory results were significantly different(P<0.001),with S2 having the optimum overall acceptability.A total of 99 volatile compounds were identified by gas chromatography-mass spectrometry,among which19 compounds were considered as the odor-active compounds according to their odor activity values.Significant changes(P<0.05)appeared in most volatiles in S0 with thyme addition,especially aldehydes.Free fatty acids(FFAs)were also identified,and all of them significantly increased with increased thyme(P<0.05).Correlation analysis of odor-active compounds,FFAs,and sensory attributes through partial least squares regression indicated the important volatiles and FFAs remarkably contributed to the mutton broth samples,and further confirmed that the 0.30%of thyme may be a desirable addition amount for the sensory characteristics of mutton broth. | Shasha Qi Ping Zhan Honglei Tian Peng Wang Xueping Ma Kaixuan Li | 2022 | Food Science and Human Wellness2022，11，2： | 2 |
| 5 | Immersing Treatment of Steel Plate Surface in Steel-Aluminum Solid to Liquid Bonding显示文摘The interfacial status of the steel-aluminum solid to liquid bonding plates (their steel plate surfaces were or were not immersed in flux aqueous solution) were measured by using SEM (Scanning Electron Microscope) and X-ray diffraction . The results showed that the layer of flux (the minimum thickness was 15 μm on the steel plate surface) could protect the steel plate surface from oxidizing effectively at high temperature in solid to liquid bonding. The melt temperatUre of the flux should be lower than 580 ℃ so that it could be melted and removed completely. No. 1 flux (patent product made by the author) made up of halogeindes could also force liquid aluminum to infiltrate into steel plate surface and thus the interfacial shear strength of the bonding plate was rather large. | Peng Zhang Yunhui Du Xueping Ren Yonglin Kang Hanwu Liu Jianzhong Cui Limin Ba (Material Science and Engineering School, University of Science and Technique Beijing, Beijing 100083, China)(Metal Forming Department Northeastern University, Shenyang 1 | 1999 | International Journal of Minerals,Metallurgy and Materials1999，13，1： | 2 |
| 6 | Free fatty acid receptor 2 promotes cardiomyocyte hypertrophy by activating STAT3 and GATA4显示文摘Free fatty acids(FFAs)play important roles in cardiovascular disease.Studies have shown that it is an important way for FAs to exert biological effects through their own receptors besides directly participating biochemical reaction in body.Free fatty acid receptor 2(FFA2)can be activated by short-chain FAs and is involved in inflammatory reactions and lipid accumulation.Since the known pathological changes caused by FFA2 are also implicated in cardiac hypertrophy,we hypothesized that FFA2 might be pathogenic in cardiac hypertrophy.This paper showed that FFA2 expression significantly increased in cardiac hypertrophy in vivo and in vitro.FFA2 agonist 4-CMTB or TUG-1375 promoted the expression of the hypertrophy markers ANF and BNP and increased cell surface area in vitro,which was further strengthened by FFA2 overexpression,suggesting that FFA2 might contribute to cardiomyocyte hypertrophy.Furthermore,4-CMTB treatment or FFA2 overexpression combined with 4-CMTB treatment elevated the phosphorylation and transcriptional activity of GATA4 and STAT3,which were inhibited by an ERK1/2 inhibitor,and GATA4 and STAT3 knockdown inhibited the elevation of hypertrophy biomarkers in cardiomyocytes treated with 4-CMTB.Taken together,these data indicate that FFA2 can enhance cardiomyocyte hypertrophy by activating STAT3 and GATA4 via ERK1/2,providing a potential new target for therapy. | Hui Gao Kunming Tian Xiaojun Feng Mengqing Yan Chen Gao Yisheng Jiang Chenhao Zhu Huzhe Zhu Xueping Liu Yingfu Peng | 2022 | Food Science and Human Wellness2022，11，2： | 1 |
| 7 | Influence of Marangoni flow on the melting process显示文摘 | Lin Xueping Peng Xiaofeng Wang Buxuan | 1997 | Prog Nat Sci1997，7，5： | 1 |
| 8 | HPA lin- earization technique of cascading PAPR reduction and predistortion显示文摘 | Xueping Li Peng Zhang Lanxiang Jiang | | 0，，10： | 1 |
| 9 | The influence of the equivalent strain on the microstructure and hardness of H62 brass subjected to multi-cycle constrained groove pressing显示文摘 | Xueping Mou Kaiping Peng Jiawei Zeng Leon L. Shaw K.-W. Qian | 2010 | Journal of Materials Processing Tech2010，，4： | 1 |
| 10 | Modeling and Optimizing of Deformed Steel Bars Hot Rolling显示文摘Deformedsteelbarsarewidelyusedinconstructionengineering.Thetensilestfengthisthemainparameterthatdetermineswhetherdeformedsteelbarsaregoodornot.Intheprocessofhotrolling,therearemanyfactorsinfluencingthetensilestrengthofdeformedsteelbars,suchasMnandStc... | Peng Zhang Yunhui Du Xueping Ren(Material Science and Engineering School, University’ of Science and Technology’ Beijing, Beijing 100083) | 1999 | International Journal of Minerals,Metallurgy and Materials1999，13，4： | 1 |
| 11 | Interpretation of Diagnosis and Treatment Protocol for Novel Coronavirus Pneumonia(Trial Version 7)显示文摘On March 3,2020,the National Health Commission and the National Administration of Traditional Chinese Medicine of China jointly promulgated the Notice on Printing and Distributing the Diagnosis and Treatment Protocol for Novel Coronavirus Pneumonia(Trial Version 7).The content has been revised and supplemented on the basis of the Version 6,including etiological characteristics and pathological changes in lung,heart,kidney,spleen,liver,gallbladder,esophagus,stomach and intestinal mucosal epithelium.In particular,the diagnosis and treatment methods of traditional Chinese medicine are supplemented.It provides an evidence-based diagnosis and treatment protocol for the treatment and recovery of suspected and confirmed cases of novel coronavirus pneumonia with traditional Chinese medicine and Western medicine. | Jinfeng CHEN Zhongyi LEI Chaofeng LIU Hong FAN Peng LEI Xueping WU Xiaoyong YU Yanfen ZHOU Jiejun HOU | 2020 | Medicinal Plant2020，11，3： | 1 |
| 12 | Deep Feature Fusion Model for Sentence Semantic Matching显示文摘Sentence semantic matching(SSM)is a fundamental research in solving natural language processing tasks such as question answering and machine translation.The latest SSM research benefits from deep learning techniques by incorporating attention mechanism to semantically match given sentences.However,how to fully capture the semantic context without losing significant features for sentence encoding is still a challenge.To address this challenge,we propose a deep feature fusion model and integrate it into the most popular deep learning architecture for sentence matching task.The integrated architecture mainly consists of embedding layer,deep feature fusion layer,matching layer and prediction layer.In addition,we also compare the commonly used loss function,and propose a novel hybrid loss function integrating MSE and cross entropy together,considering confidence interval and threshold setting to preserve the indistinguishable instances in training process.To evaluate our model performance,we experiment on two real world public data sets:LCQMC and Quora.The experiment results demonstrate that our model outperforms the most existing advanced deep learning models for sentence matching,benefited from our enhanced loss function and deep feature fusion model for capturing semantic context. | Xu Zhang Wenpeng Lu Fangfang Li Xueping Peng Ruoyu Zhang | 2019 | Computers, Materials & Continua2019，，8： | 1 |
| 13 | Extruded monolith MnO_(x)-CeO_(2)-TiO_(2) catalyst for NH_(3)-SCR of low temperature flue gas from an industry boiler:Deactivation and recovery显示文摘The selective catalytic reduction(SCR) of NO_(x) with NH_(3)(NH_(3)-SCR) technology has been widely applied for reducing NO_(x) emissions from stationary and mobile sources.In this work,the extruded monolith MnO_(x)-CeO_(2)-TiO_(2) catalyst was installed in a cement kiln for NH_(3)-SCR of NO_(x),where the flue gas temperature was 110-140℃.It is found that the monolith catalyst is severely deactivated after operating for about 200 h with almost no NO_(x) conversion at 160℃ under GHSV of 50000 h^(-1),while the fresh monolith catalyst remains 60% NO_(x) conversion.Scanning electron microscopy-energy dispersive spectroscopy(SEM-EDS),X-ray photoelectron spectroscopy(XPS),temperature-programmed desorption of SO_(2)(SO_(2)-TPD) and thermogravimetric-differential thermal analysis(TG-DTG) experiments reveal that both MnO_(x) and CeO_(2) oxides in monolith are severely sulfated to manganese sulfate and cerium sulfate,and the external monolith walls are covered by massive ceria sulfate and little ammonium nitrate.In situ diffuse reflectance infrared Fourier trans form spectroscopy(DRIFTS) analysis demonstrates that the formation of nitrates at low temperatures is inhibited due to the occupation of active sites in MnO_(x)-CeO_(2)-TiO_(2) by sulfates,resulting in the decrease of low temperature activity.After washing with water,the activity of deactivated monolith catalyst can be partially recovered,together with significant loss of manganese and cerium from monolith. | Wei Shi Jingjing Liu Ying Zhu Lin Zhao Yonggang Wang Zhaohuan Cheng Xueping Peng Xiaoyan Shi Yunbo Yu Hong He | 2023 | Journal of Rare Earths2023，41，9： | 0 |
| 14 | Influence of Tidal Cycles on Embryonic Rotation,Hatching and Emergence of Mangrove Horseshoe Crab,Carcinoscorpius rotundicauda显示文摘Horseshoe crabs are iconic and ecologically significant macroinvertebrates in coastal environments.The processes and mechanisms of larval hatching in Asian horseshoe crabs that occurs beneath the sand are largely unknown.The spawning and developmental ecology of Tachypleus tridentatus and T.gigas are assumed to be similar to their Atlantic counterpart Limulus polyphemus.However,Carcinoscorpius rotundicauda has been cited as an exception owing to their frequent sightings in muddy mangrove areas even during low tides.To reveal the larval hatching mechanisms,in this study,we examined varying hatching responses of C.rotundicauda embryos within the sediment to the environmental conditions under continuous tidal cycles.During the eight-week experiment,the count of hatched larvae ranged 4%–30%per week,while the cumulative emergence rate from the sediment was 0–47%.Embryos were observed to have the highest active rotation activity in the first two weeks after incubation.The inundation of tidal water significantly enhanced the occurrence of hatching,in which hydration,osmotic shock and possibly agitation had triggered or facilitated the eclosion.The larvae were found to remain in the sediment for approximately 2–6 weeks before emergence.In general,C.rotundicauda was found to share a similar hatching mechanism with L.polyphemus.Our findings provide insight into the developmental ecology of Asian horseshoe crabs exposed to varying tidal conditions,and are helpful to the management and protection of their spawning habitats. | KUANG Yang TAN Kian Ann FU Yijian YANG Xin XU Peng ZHEN Wenquan WANG Xueping HUANG Xing ZHU Junhua WANG Chun-Chieh KWAN Kit Yue | 2022 | Journal of Ocean University of China2022，21，3： | 0 |
| 15 | Dynamic characteristics of large permanent magnet direct‐drive generators considering electromagnetic–structural coupling effects显示文摘Dynamic characteristics of large permanent magnet direct‐drive generators(PMDGs)considering electromagnetic–structural coupling effects are analyzed in this study.Using the conformal mapping method,the scalar magnetic potential of the air gap magnetic field considering the slot effect is calculated.On the basis of the discrete current element and magnetic equivalent circuit model,the local magnetic saturation effect of the stator and rotor is quantitatively simulated and the air gap magnetic field intensity distribution is obtained via numerical simulation.A series of uniformly distributed equivalent electromagnetic springs are introduced to develop an electromagnetic–structural coupling finite element PMDG model.The proposed air gap field analysis method is verified by the finite element analysis results.On the basis of the test platform for the Goldwind 1.5MW PMDG,both modal and dynamic response tests for the stator/rotor coupling system are conducted,and the results are compared with the natural frequencies,mode shapes,and vibration responses obtained using the numerical model.The effects of the air gap length and rotor speed on the natural frequencies of the coupling system are analyzed.The proposed model has the potential to accurately evaluate the PMDG vibration energy,avoiding resonance points,and maintaining stable operations of the unit. | Qinkai Han Xueping Xu Zhihong Zhang Yifeng Yan Chao Peng Fulei Chu | 2023 | International Journal of Mechanical System Dynamics2023，3，1： | 0 |
| 16 | BTN2A2 protein ameliorates collagen-induced arthritis in mice显示文摘Rheumatoid arthritis(RA)is an autoimmune disorder characterized by persistent inflammatory responses in target tissues and organs,resulting in the destruction of joints.Collagen type II(CII)-induced arthritis(CIA)is the most used animal model for human RA.Although BTN2A2 protein has been previously shown to inhibit T cell functions in vitro,its effect on autoimmune arthritis has not been reported.In this study,we investigate the ability of a recombinant BTN2A2-IgG2a Fc(BTN2A2-Ig)fusion protein to treat CIA.We show here that administration of BTN2A2-Ig attenuates established CIA,as compared with control Ig protein treatment. | He Xueping Hu Rong Luo Peng Gao Jie Yang Wenjiang Li Jiaju Huang Youjiao Han Feng Lai Laijun Su Min | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 17 | ECG Classification Using Deep CNN Improved by Wavelet Transform显示文摘Atrial fibrillation is the most common persistent form of arrhythmia.A method based on wavelet transform combined with deep convolutional neural network is applied for automatic classification of electrocardiograms.Since the ECG signal is easily inferred,the ECG signal is decomposed into 9 kinds of subsignals with different frequency scales by wavelet function,and then wavelet reconstruction is carried out after segmented filtering to eliminate the influence of noise.A 24-layer convolution neural network is used to extract the hierarchical features by convolution kernels of different sizes,and finally the softmax classifier is used to classify them.This paper applies this method of the ECG data set provided by the 2017 PhysioNet/CINC challenge.After cross validation,this method can obtain 87.1%accuracy and the F1 score is 86.46%.Compared with the existing classification method,our proposed algorithm has higher accuracy and generalization ability for ECG signal data classification. | Yunxiang Zhao Jinyong Cheng Ping Zhang Xueping Peng | 2020 | Computers, Materials & Continua2020，，9： | 0 |
| 18 | Discussion on the Selection of ACEI and ARB for the Patients with COVID-19 and Hypertension from ACE2显示文摘With the wide spread of COVID-19,some studies have confirmed that novel coronavirus enters the cell through the binding of spike protein and ACE2 protein,which has the risk of causing the virus to enter the cell to accelerate its transmission.ACEI and ARB are the key drugs for the treatment of hypertension and are widely used in clinic.They are good for ventricular and vascular remodeling in patients with hypertension,coronary heart disease and heart failure.ACEI and ARB drugs may increase the expression of ACE2 in lung tissue and increase the risk of aggravation of the disease.According to the advice of hypertension experts:for mild ordinary COVID-19 patients with hypertension,we can consider discontinuing ACEI and ARB drugs,while for severe COVID-19 patients with hypertension,ACEI and ARB drugs should be stopped immediately.During the discontinuation period,temporary replacement therapy with diuretics,dipine and vasodilatation hypotensive drugs can be considered. | Jinfeng CHEN Zhongyi LEI Chaofeng LIU Hong FAN Peng LEI Xueping WU Xiaoyong YU Yanfen ZHOU Jiejun HOU | 2020 | Medicinal Plant2020，11，4： | 0 |
| 19 | A Novel Method for the Quantitative Detection of Sericin Content in Silk Fiber Based on the Ratio of Aspartate to Alanine显示文摘The development of a method for the quantitative determination of the sericin content ratio(SCR)is urgently needed for silk refining and the purification of silk fibroin for biomedical applications.In this work,a series of sericin/fibroin mixed samples with known SCRs were prepared by mixing initial samples of extracted sericin and fibroin from Bombyx mori silk.Significant differences were found in the contents of characteristic hydrophilic amino acids abundant in sericin and hydrophobic amino acids abundant in fibroin,and several linear relation-ships of SCR associated with the content ratios of Ser/Ala,Asp/Ala,Lys/Ala,Asp/Gly and Ser/Gly were estab-lished by amino acid analysis.Subsequently,the linear equation expressing SCR(%)as a function of the Asp to Ala content ratio X(%)was established as SCR=2.5634X−12.5587(R 2=0.9972).The results indicated that the SCR of degummed silks calculated by the equation is more objective and effective than the results obtained by the traditional weight loss method.Our study provides a novel approach for the sensitive and quantitative detection of the sericin content within the detection limit in unknown silks,which can contribute to quality control in the silk production process. | Yanfei Feng Jiaming Lin Longxing Niu Peng Pan Xueping Liu Linling Huang Ying Guo Mingzhong Li | 2023 | Journal of Renewable Materials2023，11，1： | 0 |
| 20 | Discussion on the Prevention and Treatment of COVID-19 Causing Lung Disease and Heart Damage Based on Lei Zhongyi's Theory of Intermingled Phlegm,Blood Stasis and Toxin显示文摘Novel coronavirus infection not only damages lung function,but also causes myocardial injury,elevated myocardial enzymes and heart failure,especially for patients with basic heart diseases who develop COVID-19,the first consideration should be the protection of cardiac function.Based on the theory of intermingled phlegm,blood stasis and toxin of heart disease put forward by Master Lei Zhongyi,the dialectical treatment thinking of COVID-19 patients from the concept of damage of phlegm,blood stasis and toxin to the heart were discussed.During the diagnosis,critical stage and recovery period of COVID-19,expectorant and blood-activating agents,heat and detoxification agents can be added to promote lung and asthma,free Bizheng and remove blood stasis,calm the heart and calm the mind,and promote the recovery of cardiopulmonary functions. | Jinfeng CHEN Zhongyi LEI Chaofeng LIU Hong FAN Peng LEI Xueping WU Xiaoyong YU Yanfen ZHOU Jiejun HOU | 2020 | Medicinal Plant2020，11，6： | 0 |
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