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| 1 | Improved Base Editor for Efficiently Inducing Genetic Variations in Rice with CRISPR/Cas9- Guided Hyperactive hAID Mutant显示文摘 | Bin Ren Fang Yan Yongjie Kuang Na Li Dawei Zhang Xueping Zhou Honghui Lin Huanbin Zhou | 2018 | Molecular Plant2018，11，4： | 46 |
| 2 | Highly Efficient A. T to G. C Base Editing by Cas9n- 3uided tRNA Adenosine Deaminase in Rice显示文摘 | Fang Yan Yongjie Kuang Bin Ren Jingwen Wang Dawei Zhang Honghui Lin Bing Yang Xueping Zhou Huanbin Zhou | 2018 | Molecular Plant2018，11，4： | 26 |
| 3 | Cas9-NG Greatly Expands the Targeting Scope of the Genome-Editing Toolkit by Recognizing NG and Other Atypical PAMs in Rice显示文摘CRISPR technologies enabling precise genome manipulation are valuable for gene function studies and molecular crop breeding. However, the requirement of a protospacer adjacent motif (PAM)y such as NGG and TTN, for Cas protein recognition restricts the selection of targetable genomic loci in practical applications of CRISPR technologies. Recently Cas9-NG, which recognizes a minimal NG PAM, was reported to expand the targeting space of genome editing in human cells, but it remains unclear whether this Cas9 variant can be used in plants. In this study, we evaluated the nuclease activity of Cas9-NG toward various NGN PAMs by targeting endogenous genes in transgenic rice. We found that Cas9-NG edits all NGG, NGA, NGT, and NGC sites with impaired activity, while the gene-edited plants were dominated by monoallelic mutations. Cas9-NG-engineered base editors were then developed and used to generate O s B Z R I gainof- function plants that can not be created by other available Cas9-engineered base editors. Moreover, we showed that a Cas9-NG-based transcriptional activator efficiently upregulated the expression of endogenous target genes in rice. In addition, we discovered that Cas9-NG recognizes NAC, NTG, NTT, and NCG apart from NG PAM. Together, these findings demonstrate that Cas9-NG can greatly expand the targeting scope of genome-editing tools, showing great potential for targeted genome editing, base editing, and genome regulation in plants. | Bin Ren Lang Liu Shaofang Li Yongjie Kuang Jingwen Wang Dawei Zhang Xueping Zhou Honghui Lin Huanbin Zhou | 2019 | Molecular Plant2019，12，7： | 18 |
| 4 | Quasi-solid-state nanocrystalline TiO2 solar cells using gel network polymer electrolytes based on polysiloxanes显示文摘A quasi-solid-state dye-sensitized nanocrystal-line porous TiO2 film solar cell was fabricated using a novel gel network polymer electrolyte based on polysiloxanes with both polyethylene oxide internal plasticized side chains and quaternary ammonium groups. The cell exhibited better photoelectrical conversion performance under 60 mW/cm2 irradiation. The short photocurrent (Isc) of 5.0 mA/cm2 and open voltage (Voc) of 0.68 V were achieved, and the energy conversion efficiency (h ) and fill factor (ff) were 3.4% and 0.60, respectively. | LI Weiying, KANG Junjie, LI Xueping, FANG Shibi, LIN Yuan, WANG Guiqiang & XIAO Xurui Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China | 2003 | Chinese Science Bulletin2003，48，7： | 11 |
| 5 | The fabrication of biomimetic biphasic CAN-PAC hydrogel with a seamless interfacial layer applied in osteochondral defect repair显示文摘Cartilage tissue engineering based on biomimetic scaffolds has become a rapidly developing strategy for repairing cartilage defects. In this study, a biphasic CAN-PAC hydrogel for osteochondral defect(OCD)regeneration was fabricated based on the density difference between the two layers via a thermally reactive,rapid cross-linking method. The upper hydrogel was cross-linked by CSMA and NIPAm, and the lower hydrogel was composed of PECDA, AAm and PEGDA. The interface between the two layers was first grafted by the physical cross-linking of calcium gluconate and alginate, followed by the chemical cross-linking of the carbon-carbon double bonds in the other components. The pore sizes of the upper and lower hydrogels were ~ 187.4 and ~ 112.6 μm, respectively. The moduli of the upper and lower hydrogels were ~ 0.065 and~ 0.261 MPa. This prepared bilayer hydrogel exhibited the characteristics of mimetic composition, mimetic structure and mimetic stiffness, which provided a microenvironment for sustaining cell attachment and viability. Meanwhile, the biodegradability and biocompatibility of the CAN-PAC hydrogel were examined in vivo. Furthermore, an osteochondral defect model was developed in rabbits, and the bilayer hydrogels were implanted into the defect. The regenerated tissues in the bilayer hydrogel group exhibited new translucent cartilage and repaired subchondral bone, indicating that the hydrogel can enhance the repair of osteochondral defects. | Jinfeng Liao Taoran Tian Sirong Shi Xueping Xie Quanquan Ma Guo Li Yunfeng Lin | 2017 | Bone Research2017，5，2： | 10 |
| 6 | Co-occurrence Patterns of Above-ground and Below-ground Mite Communities in Farmland of Sanjiang Plain, Northeast China显示文摘One of the fundamental questions in community ecology is whether communities are random or formed by deterministic mechanisms. Although many efforts have been made to verify non-randomness in community structure, little is known with regard to co-occurrence patterns in above-ground and below-ground communities. In this paper, we used a null model to test non-randomness in the structure of the above-ground and below-ground mite communities in farmland of the Sanjiang Plain, Northeast China. Then, we used four tests for non-randomness to recognize species pairs that would be demonstrated as significantly aggregated or segregated co-occurrences of the above-ground and below-ground mite communities. The co-occurrence pattern of the above-ground mite community was significantly non-random in October, suggesting species segregation and hence interspecific competition. Additionally, species co-occurrence patterns did not differ from randomness in the above-ground mite community in August or in below-ground mite communities in August and October. Only one significant species pair was detected in the above-ground mite community in August, while no significant species pairs were recognized in the above-ground mite community in October or in the below-ground mite communities in August and October. The results indicate that non-randomness and significant species pairs may not be the general rule in the above-ground and below-ground mite communities in farmland of the Sanjiang Plain at the fine scale. | LIN Lin GAO Meixiang LIU Dong ZHANG Xueping WU Haitao WU Donghui | 2014 | Chinese Geographical Science2014，24，3： | 7 |
| 7 | Ability to detect weak effective seismic signals by utilizing chaotic vibrator system显示文摘Taking the advantage of CVS (Chaotic Vibrator System) sensitivity of large-scale periodic phase-state response to quasi-periodic or periodic signals,a series of numerical experiments were made to understand the ability of CVS to detect weak effective seismic signals in the common-shot seismic record distorted by strong stochastic noise. The re-sults demonstrate that the large-scale periodic phase-states of CVS are correlated with the signal composition of the quasi-periodic wavelet sequence constructing from horizontal moveout of seismic events,noise strength and the noise distortion de-gree to signal. For the same kind of events,the higher the noise distortion degree is,the lower the detectable SNR can be reached by CVS. For seismic data with the same noise distortion degree,the closer the scanning seismic velocity (the trial moveout ve-locity) approaches to the accurate velocity,the higher the detectable SNR can be reached by CVS. More-over,the truncating scanning velocities form an asymmetric belt,which indirectly makes CVS achieve a large-scale periodic phase-state and then the ratio of wavelet distortion coefficients in events can be a biggish variable scope. | LI Yue YANG Baojun YUAN Ye ZHAO Xueping LIN Hongbo | 2006 | Chinese Science Bulletin2006，51，24： | 6 |
| 8 | Synthesis of TiO_(2) nanotubes film and its light scattering property显示文摘TiO2 nanotubes with diameters of 10 nm and lengths up to 600 nm were fabricated by directly using com-mercial TiO2 powders P25 as the precursors via sonica-tion-hydrothermal combination approach. TiO2 nanotubes were characterized by means of X-ray powder diffractometer (XRD), scanning electron microscope (SEM), selected area electron diffraction pattern (SAED) and transmission elec-tron microscope (TEM). The light scattering property of film electrodes modified with TiO2 nanotubes was studied and revealed that TiO2 nanotubes can be used as the light scat-tering centers to increase the light absorption in dye- sensi-tized solar cells. The TiO2 nanotubes film electrodes mixed with 10% small nanoparticles TiO2 had both strong light scattering property and fine mechanical characteristics, and this kind of electrodes can be used as electrodes in improving the conversion efficiencies of dye-sensitized solar cells. | MA Yutao LIN Yuan XIAO Xurui LI Xueping ZHOU Xiaowen | 2005 | Chinese Science Bulletin2005，50，18： | 6 |
| 9 | Nanocrystalline TiO_(2) thin film electrodes prepared by common pressure hydrothermal method at low temperature显示文摘Nanocrystalline TiO2 thin film electrodes have been prepared from mixed pastes of tetrabutyl titanate and nanocrystalline TiO2 particles by common pressure hydro- thermal method at low temperature. The tetrabutyl titanate was hydrolyzed and crystallized into anatase TiO2 to inter- connect nanocrystalline TiO2 particles and adhere them to conductive substrates, obtaining highly porous and me- chanically stable TiO2 nanocrystalline film. The conversion efficiencies of the dye-sensitized solar cells based on prepared electrodes on conductive glass substrates and flexible sub- strates were 4.8% and 1.9% under illumination of 100 mW/cm2, respectively. | LI Chengyu LIN Yuan LI Xueping WANG Zhengping MA Yutao ZHOU Xiaowen FENG Shujing XIAO Xurui | 2005 | Chinese Science Bulletin2005，50，14： | 5 |
| 10 | A new type quasi-solid state electrolyte for dye-sensitized solar cells显示文摘A new type quasi-solid state electrolyte was prepared by solidifying liquid electrolytes con- taining organic solvents (such as mixture of ethylene carbonate (EC) and propylene carbonate (PC), 3- methoxypropinitrile (NMP) and N-methyl-oxazolidin- one (NMO)) with comb-like molten salt type polymer, and was for the first time employed in dyesensitized solar cells (DSSCs). The optimal electrolyte compo- sition was obtained by regulating the polymer content in the electrolytes and optimizing performance data of the electrolytes and assembled cells, yielding a maximum conversion efficiency of 6.58% (AM 1.5, 100 mW·cm?2). Furthermore, the existence of this new type polymer in the electrolyte suppresses the evaporation of organic solvent and improves the sta- bility of the cells. | WANG Miao YANG Lei ZHOU Xiaowen LIN Yuan LI Xueping FENG Shujing XIAO Xurui | 2006 | Chinese Science Bulletin2006，51，13： | 4 |
| 11 | Nucleic acids and analogs for bone regeneration显示文摘With the incidence of different bone diseases increasing, effective therapies are needed that coordinate a combination of various technologies and biological materials. Bone tissue engineering has also been considered as a promising strategy to repair various bone defects. Therefore, different biological materials that can promote stem cell proliferation, migration, and osteoblastic differentiation to accelerate bone tissue regeneration and repair have also become the focus of research in multiple fields. Stem cell therapy, biomaterial scaffolds, and biological growth factors have shown potential for bone tissue engineering; however, off-target effects and cytotoxicity have limited their clinical use. The application of nucleic acids(deoxyribonucleic acid or ribonucleic acid)and nucleic acid analogs(peptide nucleic acids or locked nucleic acids), which are designed based on foreign genes or with special structures, can be taken up by target cells to exert different effects such as modulating protein expression, replacing a missing gene, or targeting specific gens or proteins. Due to some drawbacks, nucleic acids and nucleic acid analogs are combined with various delivery systems to exert enhanced effects, but current studies of these molecules have not yet satisfied clinical requirements. In-depth studies of nucleic acid or nucleic acid analog delivery systems have been performed, with a particular focus on bone tissue regeneration and repair. In this review, we mainly introduce delivery systems for nucleic acids and nucleic acid analogs and their applications in bone repair and regeneration. At the same time, the application of conventional scaffold materials for the delivery of nucleic acids and nucleic acid analogs is also discussed. | Yuxin Zhang Wenjuan Ma Yuxi Zhan Chenchen Mao Xiaoru Shao Xueping Xie Xiawei Wei Yunfeng Lin | 2018 | Bone Research2018，6，4： | 3 |
| 12 | Multi?targeted Antisense Oligonucleotide Delivery by a Framework Nucleic Acid for Inhibiting Biofilm Formation and Virulence显示文摘Biofilm formation is responsible for numerous chronic infections and represents a serious health challenge.Bacteria and the extracellular polysaccharides(EPS)cause biofilms to become adherent,toxic,resistant to antibiotics,and ultimately difficult to remove.Inhibition of EPS synthesis can prevent the formation of bacterial biofilms,reduce their robustness,and promote removal.Here,we have developed a framework nucleic acid delivery system with a tetrahedral configuration.It can easily access bacterial cells and functions by delivering antisense oligonucleotides that target specific genes.We designed antisense oligonucleotide sequences with multiple targets based on conserved regions of the VicK protein-binding site.Once delivered to bacterial cells,they significantly decreased EPS synthesis and biofilm thickness.Compared to existing approaches,this system is highly efficacious because it simultaneously reduces the expression of all targeted genes(gtfBCD,gbpB,ftf).We demonstrate a novel nucleic acid-based nanomaterial with multi-targeted inhibition that has great potential for the treatment of chronic infections caused by biofilms. | Yuxin Zhang Xueping Xie Wenjuan Ma Yuxi Zhan Chenchen Mao Xiaoru Shao Yunfeng Lin | 2020 | Nano-Micro Letters2020，12，6： | 3 |
| 13 | Mild hypothermia effects on matrix metalloproteinase-9 expression in the perihematomal region of rats following experimental intracerebral hemorrhage显示文摘BACKGROUND: Matrix metalloproteinase-9 (MMP-9) expression increases with intracerebral hemorrhage, and participates in the pathophysiological processes of secondary brain injury after intracerebral hemorrhage. OBJECTIVE: To investigate the effects of mild hypothermia on MMP-9 expression and brain edema in the perihematomal region of experimental intracerebral hemorrhage rats. DESIGN, TIME AND SETTING: The randomized, controlled experiment was performed at the Central Laboratory of Shandong Provincial Hospital between May and September 2007. MATERIALS: Seventy-two, Wistar, male rats, 12-weeks old, were used for this study. Rabbit anti-MMP-9 primary antibody was purchased from Boster, China. METHODS: Wistar rats were equally and randomly divided into normothermia and mild hypothermia groups. The two groups each comprised control, 6-hour intracerebral hemorrhage, 24-hour intracerebral hemorrhage, 48-hour intracerebral hemorrhage, 72-hour intracerebral hemorrhage, and 1-week intracerebral hemorrhage subgroups, with six rats in each subgroup. Rat models of intracerebral hemorrhage were established by injecting 100 μL of autologous blood into the rat caudate nucleus. Rats in the mild hypothermia group received four hours of local mild hypothermia immediately following the injection. Intracerebral temperature was maintained at (33 ± 0.5) ℃. Subsequently, intracerebral temperature was spontaneously recovered at 25 ℃. Rats in the control subgroup were not injected with autologous blood and received only with intracerebral hemorrhage. MAIN OUTCOME MEASURES: Brain water content and MMP-9 expression surrounding the hematoma region. RESULTS: MMP-9 expression increased at 6 hours, and brain edema reached a peak at 48 hours after intracerebral hemorrhage. MMP-9 expression was significantly decreased in the mild hypothermia group compared with the normothermia group at each time point (P < 0.05). CONCLUSION: Mild hypothermia can significantly inhibit MMP-9 overexpression and relieve brain edema following intracerebral hemorrhage. | Minzhong Wang Xiaoyan Lin Xueping Liu Qingxi Fu Suming Zhang | 2008 | Neural Regeneration Research2008，3，7： | 2 |
| 14 | The biological applications of DNA nanomaterials:current challenges and future directions显示文摘DNA,a genetic material,has been employed in different scientific directions for various biological applications as driven by DNA nanotechnology in the past decades,including tissue regeneration,disease prevention,inflammation inhibition,bioimaging,biosensing,diagnosis,antitumor drug delivery,and therapeutics.With the rapid progress in DNA nanotechnology,multitudinous DNA nanomaterials have been designed with different shape and size based on the classic Watson–Crick base-pairing for molecular self-assembly.Some DNA materials could functionally change cell biological behaviors,such as cell migration,cell proliferation,cell differentiation,autophagy,and anti-inflammatory effects.Some single-stranded DNAs(ssDNAs)or RNAs with secondary structures via self-pairing,named aptamer,possess the ability of targeting,which are selected by systematic evolution of ligands by exponential enrichment(SELEX)and applied for tumor targeted diagnosis and treatment.Some DNA nanomaterials with three-dimensional(3D)nanostructures and stable structures are investigated as drug carrier systems to delivery multiple antitumor medicine or gene therapeutic agents.While the functional DNA nanostructures have promoted the development of the DNA nanotechnology with innovative designs and preparation strategies,and also proved with great potential in the biological and medical use,there is still a long way to go for the eventual application of DNA materials in real life.Here in this review,we conducted a comprehensive survey of the structural development history of various DNA nanomaterials,introduced the principles of different DNA nanomaterials,summarized their biological applications in different fields,and discussed the current challenges and further directions that could help to achieve their applications in the future. | Wenjuan Ma Yuxi Zhan Yuxin Zhang Chenchen Mao Xueping Xie Yunfeng Lin | 2021 | Signal Transduction and Targeted Therapy2021，6，11： | 2 |
| 15 | Preparation of porous nanocrystalline TiO_2 electrode by screen-printing technique显示文摘Different paste has been used for preparing porous TiO2 thin film by screen-printing technique, the main component of it comes from commercial TiO2 P25 power. The dye-sensitized solar cell based on this TiO2 thin film without further chemical treatments exhibits high overall conversion efficiency of 5.81%―6.70%, even with low TiO2 content and thin film thickness. The experimental repeatability is nice and the properties of the films are uniform. | XIE DongMei FENG ShuJing LIN Yuan DONG GuoJun XIAO XuRui LI XuePing ZHOU XiaoWen | 2007 | Chinese Science Bulletin2007，52，18： | 2 |
| 16 | The effects of low-intensity pulsed ultrasound and nanomagnet applications on the expressions of MMP-13 and MAPKs in rabbit knee osteoarthritis显示文摘 | Li Xueping Lin Qiang Wang Daxin | 2013 | Journal of Nanoscience and Nanotechnology2013，13，1： | 1 |
| 17 | Preparation of nanocrystalline TiO2 thin film at low temperature and its application in dye-sensitized solar cell显示文摘 | Weiwei Tan Jinmao Chen Xiaowen Zhou Jingbo Zhang Yuan Lin Xueping Li Xurui Xiao | 2009 | Journal of Solid State Electrochemistry2009，，5： | 1 |
| 18 | Influence of Marangoni flow on the melting process显示文摘 | Lin Xueping Peng Xiaofeng Wang Buxuan | 1997 | Prog Nat Sci1997，7，5： | 1 |
| 19 | Ratios of regulatory T cells/T‐helper 17 cells and transforming growth factor‐β1/interleukin‐17 to be associated with the development of hepatitis B virus‐associated liver cirrhosis显示文摘 | Xueping Yu Ruyi Guo Desong Ming Milong Su Chengzu Lin Yong Deng Zhenzhong Lin Zhijun Su | 2014 | J Gastroenterol Hepatol2014，，5： | 1 |
| 20 | Interception, degradation and contributions of terrestrial organic carbon obtained from lignin analysis in Wujiang River, southwest China显示文摘Understanding the fate of terrestrial organic carbon(OC)in a cascade impoundment system is critical for recognizing the role of carbon sink for reservoirs.Surface sediments collected from eight cascade reservoirs across the Wujiang River,southwestern China,were analyzed for elemental and stable carbon isotopic(δ^13C)composition,and lignin phenols(∑8 andΛ8)to investigate the spatial distribution,contribution,origin and degradation of sedimentary terrestrial OC.The values of total organic carbon(TOC)and∑8 exhibited a remarkable reduction along the upstream-downstream transect suggesting the trapping effect of cascade-damming.A relatively broad range ofδ^13C(-26.61 to-25.54%,95%CI)and C/N(6.80-18.20)indicated mixed allochthonous/autochthonous OC sources in surface sediments.The quantitative simulation indicates that the OC of the sediments mainly was derived from terrestrial organic matter.Soilderived OC rather than C_(3)vascular plant-derived OC makes a major contribution to sedimentary terrestrial OC in reservoirs on karst terrain.As evidenced by lignin compositions andδ^13C,the predominant vascular plant origins of terrestrial OC along the Wujiang River are non-woody angiosperm C_(3)plants.The aged reservoirs showed a trend of increasing contribution of autochthonous OC,which potentially weaken the role of carbon sink for reservoirs.The relationship between runoff inputs,watershed area/water surface area ratios,and water residence time and Λ8 were explored,indicating the natural and anthropogenic influences on terrestrial OC remains very complex in a cascade-damming river. | Xin Lin Yujie Wang Jinhua Zhang Ming Yang Xueping Chen Fushun Wang Jing Ma | 2021 | Acta Geochimica2021，40，6： | 1 |
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