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| 1 | Ythdc2 is an N^6-methyladenosine binding protein that regulates mammalian spermatogenesis显示文摘N 6-methyladenosine (m 6 一) 是在真核细胞的 mRNA 的最普通的内部修正。它动态地被安装并且搬迁,并且充当 mRNA 新陈代谢,包括干细胞 pluripotency 的调整生物过程,房间区别,和精力动态平衡的新层。m 6 A 被选择有约束力的蛋白质认出;YTHDF1 和 YTHDF3 在音乐会工作影响 m 6包含 A 的 mRNAs, YTHDF2 帮助 mRNA 腐烂,和 YTHDC1 影响它的目标的原子处理。YTHDC2 的生物功能, YTH 蛋白质家庭,的最后的成员仍然保持未知。我们报导 YTHDC2 有选择地绑 m 6 在它的一致主题的 A。YTHDC2 提高它的目标的翻译效率并且也减少他们的 mRNA 丰富。Ythdc2 猛烈老鼠是不肥沃的;男性们让显著地更小的睾丸和女性同窝出生的人与那些相比有显著地更小的卵巢。Ythdc2 猛烈老鼠的细菌房间不经过 zygotene 阶段发展,因此,当成熟分裂开始, Ythdc2 是在睾丸的 upregulated。因此, YTHDC2 是 m 6 在精子发生期间起关键作用的 A 绑定蛋白质。 | Phillip J Hsu Yunfei Zhu Honghui Ma Yueshuai Guo Xiaodan Shi Yuanyuan Liu Meijie Qi Zhike Lu Hailing Shi Jianying Wang Yiwei Cheng Guanzheng Luo Qing Dai Mingxi Liu Xuejiang Guo Jiahao Sha Bin Shen Chuan He | 2017 | Cell Research2017，27，9： | 96 |
| 2 | Atomization Inhalation of Terbutaline and Budesonide Efficiently Improved Immunity and Lung Function of AECOPD Patients显示文摘Chronic obstructive pulmonary disease (COPD) is a syndrome of chronic progressive airflow limitation as a result of chronic inflammation of the airways and lung parenchyma. COPD patients always have airway hyperreactivity (AHR),so how to reduce AHR becomes the key purpose of clinical treatment. It is hypothesized that combined inhalation of corticosteroids and β2-agonists can reduce the AHR in COPD. In this study,atomization inhalation of budesonide and terbutaline plus conventional therapies was applied to treat AECOPD (acute exacerbation of chronic obstructive pulmonary disease) patients for two weeks. The results showed that additional inhalation of budesonide and terbutaline could upregulate serum IL-2 levels,the percentages of CD3+ T and CD4+ T cells,and CD4/CD8 ratio,and decrease eosinophils and serum CRP level more efficiently than conventional treatment in patients with AECOPD. And the lung function of the atomization inhalation group was improved more obviously after the treatment compared with the conventional treatment group. Thus,atomization inhalation of terbutaline and budesonide can control AECOPD effectively,and has wide clinical perspective in controlling and preventing the exacerbation of COPD. | Guilan Xiong Lijun Xu Liu Wei Xiaodan Li | 2008 | Cellular & Molecular Immunology2008，5，4： | 30 |
| 3 | A preventive control strategy for static voltage stability based on an efficient power plant model of electric vehicles显示文摘With the increasing integration of wind farms and electric vehicles(EVs)in power systems,voltage stability is becoming more and more serious.Based on vehicle-to-grid(V2G),an efficient power plant model of EVs(E-EPP)was developed to estimate EV charging load with available corresponding response capacity under different charging strategies.A preventive control strategy based on E-EPP was proposed to maintain the static voltage stability margin(VSM)of power system above a predefined security level.Two control modes were used including the disconnection of EV charging load(‘V1G’mode)and the discharge of stored battery energy back to power grid(‘V2G’mode).A modified IEEE 14-bus system with high penetration of wind power and EVs was used to verify the effectiveness of preventive control strategy.Simulation results showed that the proposed strategy can not only improve the static voltage stability of power system with considerable wind generation,but also guarantee the travelling comfort for EV owners. | Mingshen WANG Yunfei MU Hongjie JIA Jianzhong WU Xiuping YAO Xiaodan YU Janaka EKANAYAKE | 2015 | Journal of Modern Power Systems and Clean Energy2015，3，1： | 25 |
| 4 | 糖尿病视网膜病变患病率的流行病学调查:在中国6省13473名糖尿病患者中的横断面研究显示文摘目的本研究旨在探究中国糖尿病患者中糖尿病视网膜病变(DR)和视力威胁性糖尿病视网膜病变(STDR)的患病率和严重程度。研究设计、设置与参与者本研究为横断面流行病学调查,研究对象为中国内地地区的糖尿病患者(N=13473)。研究对象为被内科医师诊断为糖尿病,并转诊到筛查门诊的糖尿病患者(其中住院患者占1/3,城市居民占1/3,农村居民占1/3)。研究对象的每只眼睛应用彩色、免散瞳和非立体眼底照相机拍摄2张眼底照片,并根据英国DR分级指南进行分级。主要结果和测量指标DR与STDR的患病率和严重程度。结果在参与本研究的13473例糖尿病患者中,共发现4591例DR和1769例STDR患者,DR的总体患病率为34.08%[95%可信区间(凹)33.28%~34.88%],其中STDR的总体患病率为13.13%(95%C112.56%~13.70%)。在所有研究对象中,12780例参与了眼底照片分级(94.86%)。年龄大于65岁的研究对象患DR或STDR的可能性较低(P〈0.001)。性别对是否患病没有显著影响(P〉0.05)。STDR患者与非STDR患者相比,视力损伤更为严重(P〈0.001)。中国北方DR和STDR的患病率高于南方(P〈0.001)。DR的患病率与糖尿病患病时间f比值比(鲫)2.63;95%C12.42~2.86;P〈0.001]以及糖尿病发病年龄(OR 0.38;95%CI为0.35~0.41;P〈0.001)密切相关。结论DR和STDR在中国内地地区的患病率偏高,并且不同地区患病率存在差异。在DR中,非增殖性DR更常见,但STDR更需要及时的诊断和治疗,特别是在经济欠发达地区。本研究强调了中国内地地区DR筛查和治疗的重要性。 | Liu, Yan Song, Yifan Tao, Liyuan Qiu, Weiqiang Lv, Huibin Jiang, Xiaodan Zhang, Mingzhou Li, Xuemin | 2017 | 英国医学杂志中文版2017，20，11： | 23 |
| 5 | Osteoimmunomodulatory effects of biomaterial modification strategies on macrophage polarization and bone regeneration显示文摘Biomaterials as bone substitutes are always considered as foreign bodies that can trigger host immune responses.Traditional designing principles have been always aimed at minimizing the immune reactions by fabricating inert biomaterials.However,clinical evidence revealed that those methods still have limitations and many of which were only feasible in the laboratory.Currently,osteoimmunology,the very pioneering concept is drawing more and more attention-it does not simply regard the immune response as an obstacle during bone healing but emphasizes the intimate relationship of the immune and skeletal system,which includes diverse cells,cytokines,and signaling pathways.Properties of biomaterials like topography,wettability,surface charge,the release of cytokines,mediators,ions and other bioactive molecules can impose effects on immune responses to interfere with the skeletal system.Based on the bone formation mechanisms,the designing methods of the biomaterials change from immune evasive to immune reprogramming.Here,we discuss the osteoimmunomodulatory effects of the new modification strategies—adjusting properties of bone biomaterials to induce a favorable osteoimmune environment.Such strategies showed potential to benefit the development of bone materials and lay a solid foundation for the future clinical application. | Yajuan Xie Cheng Hu Yi Feng Danfeng Li Tingting Ai Yulei Huang Xiaodan Chen Lijia Huang Jiali Tan | 2020 | Regenerative Biomaterials2020，7，3： | 22 |
| 6 | Hierarchical 3D mesoporous silicon@graphene nanoarchitectures for lithium ion batteries with superior performance显示文摘硅为高能力锂离子电池作为最有希望的阳极材料被认出了。然而,大体积变化在骑车上在充电和分泌物期间导致 Si 电极和快能力损失的弄成粉。Si 电极的这个缺点能被联合与组织良好的 graphene 泡沫克服。在这个工作,层次三维的碳涂的 mesoporous Si nanospheres@graphene 泡沫(C@Si@GF ) nanoarchitectures 被热水泡喷射成功地综合帮助 chemical-vapor-deposition 和 magnesiothermic 减小方法。同样准备的 nanocomposites 的形态学和结构被扫描电子显微镜学,传播电子显微镜学和拉曼光谱学的地排放描绘。当在锂离子电池作为阳极材料采用了时, C@Si@GF nanocomposites 在 1 A/g,优秀的高率能力和突出的 cyclability 的当前的密度包括 1,200 mAh/g 的一个高特定的能力展出了优异电气化学的性能。验尸,分析鉴别在 200 个周期以后的 3D C@Si@GF 电极的形态学很好被维持。从 mesoporous Si nanospheres 和 graphene 泡沫 nanoarchitectures 的联合产生的 synergistic 效果可以处理 Si 电极的难处理的弄成粉问题。 | Shuangqiang Chen Peite Bao Xiaodan Huang Bing Sun Guoxiu Wang | 2014 | Nano Research2014，7，1： | 19 |
| 7 | Progress in Semi-arid Climate Change Studies in China显示文摘This article reviews recent progress in semi-arid climate change research in China.Results indicate that the areas of semiarid regions have increased rapidly during recent years in China,with an increase of 33%during 1994-2008 compared to 1948-62.Studies have found that the expansion rate of semi-arid areas over China is nearly 10 times higher than that of arid and sub-humid areas,and is mainly transformed from sub-humid/humid regions.Meanwhile,the greatest warming during the past 100 years has been observed over semi-arid regions in China,and mainly induced by radiatively forced processes.The intensity of the regional temperature response over semi-arid regions has been amplified by land-atmosphere interactions and human activities.The decadal climate variation in semi-arid regions is modulated by oceanic oscillations,which induce land-sea and north-south thermal contrasts and affect the intensities of westerlies,planetary waves and blocking frequencies.In addition,the drier climates in semi-arid regions across China are also associated with the weakened East Asian summer monsoon in recent years.Moreover,dust aerosols in semi-arid regions may have altered precipitation by affecting the local energy and hydrological cycles.Finally,semi-arid regions in China are projected to continuously expand in the 21st century,which will increase the risk of desertification in the near future. | Jianping HUANG Jieru MA Xiaodan GUAN Yue LI Yongli HE | 2019 | Advances in Atmospheric Sciences2019，36，9： | 15 |
| 8 | A Host KH RNA-Binding Protein Is a Susceptibility Factor Targeted by an RXLR Effector to Promote Late Blight Disease显示文摘植物病原体交付改变主人过程创造对殖民导致的环境的受动器蛋白质。注意集中了于识别受动器的目标并且他们的操作怎么便于疾病。从土豆老家病原体 Phytophthora infestans 的 RXLR 受动器 Pi04089 在主人原子核积累并且当短暂地在 planta 表示了时,提高殖民。它的原子本地化为提高的 P.infestans 殖民被要求。Pi04089 与通常认为的土豆 K 相同(KH ) 在酵母并且在 planta 交往 RNA 有约束力的蛋白质, StKRBP1。Pi04089 和 StKRBP1 的合作本地化,和在他们之间的双分子的荧光互补,显示他们在原子点缀联系。当它与 Pi04089 被共同表示时, StKRBP1 蛋白质层次增加了。确实, StKRBP1 的如此的累积被病原体在叶殖民的第一天也观察。显著地, StKRBP1 的 overexpression 显著地提高 P.infestans 感染。当与受动器共同表示时,到在 StKRBP1 的所有三个 KH 领域的 GDDG 的核苷酸绑定主题 GxxG 的变化与 Pi04089,它到原子点缀的本地化,和它的增加的累积废除它的相互作用。而且,变异的 StKRBP1 蛋白质不再由 P.infestans 提高叶殖民,暗示那核苷酸绑定多半为这项活动被要求。我们因此主张 StKRBP1 能被认为是一个危险性因素,因为它的活动对病原体有益。 | Xiaodan Wang Petra Boevink Hazel McLellan Miles Armstrong Tatyana Bukh-arova Zhiwei Qin Paul R.J. Birch | 2015 | Molecular Plant2015，8，9： | 15 |
| 9 | Climate indexes of phytoliths from Homo erectus' cave deposits in Nanjing显示文摘A study on phytoliths and their climate indexes is carried out from Homo erectus?cave deposits in HuluCave, Nanjing. Evidence shows that phytolith assemblages of the cave deposits are dominated by the cold resistant types with a lower warm index, reflecting an overall cold inclined climate. This possibly connects the cave deposits with glacial climate to a great extent, which is in accordance with thenorthern fauna revealed by fossil mammals and temperateclimate indicated by pollen assemblages. According to the distributional state of the phytoliths and their climate in-dexes on 4 profiles in the cave, it is revealed that profiles Ⅰand Ⅱ display certain cold/warm, and dry/wet fluctuations; profile Ⅲ shows a humid and cold condition with the high-est humility in the cave deposits; while profile Ⅳ indicates a possible quick accumulating process because of its stableclimate indexes except for its bottom and top. | Weiming Wang Jinling Liu Xiaodan Zhou | 2003 | Chinese Science Bulletin2003，48，18： | 14 |
| 10 | Influence of Ren and Du meridian electro-acupuncture on neural stem cell proliferation and extracellular signal-regulated kinase pathway in a rat model of focal cerebral ischemia injury显示文摘BACKGROUND:Studies have shown that electro-acupuncture at the Ren meridian could improve proliferation of subventricular zone neural stem cells in cerebral-ischemic rats. However,there are few reports on the influence of electro-acupuncture at the Du meridian on neural stem cell proliferation. OBJECTIVE:To observe the influence of electro-acupuncture at Ren and Du meridians on neural stem cell proliferation in the subventricular zone and altered signal transduction in cerebral ischemia rats. DESIGN,TIME AND SETTING:A randomized,controlled,animal experiment was performed at the Laboratory of Human Anatomy,Medical College of Sun Yat-sen University from May 2006 to February 2008. MATERIALS:Mouse anti-rat bromodeoxyuridine (BrdU) monoclonal antibody was provided by Sigma,USA; mouse anti-rat nestin monoclonal antibody and extracellular signal-regulated protein kinase (ERK) specific inhibitor PD98059 were provided by Calbiochem,Germany; acupuncture needle was provided by Suzhou Acupuncture Supplies,China. METHODS:A total of 126 rats were randomly assigned to four groups:model (n = 36),Du meridian (n = 36),Ren/Du meridian (n = 36),and Ren/Du meridian + PD98059 (n = 18). Rats in the Ren /Du meridian + PD98059 group were observed on days 7 (n = 6) and 14 (n = 12) after cerebral ischemia injury. Rats in the model,Du meridian,and Ren/Du meridian groups were observed on days 7,14,and 28 after cerebral ischemia injury,with 12 rats per group at each time point. Thread occlusion was used to establish middle cerebral artery occlusion models. Electro-acupuncture was performed at Renzhong (DU 26) and Baihui (DU 20) acupoints in the Du meridian group,as well as Chengjiang (RN 24),Guanyuan (RN 4),Renzhong,and Baihui acupoints in the Ren/Du meridian and Ren/Du meridian + PD98059 groups 2 days after model establishment. In addition,electro-acupuncture stimulation with disperse-dense waves was performed,with 30 Hz disperse wave,100 Hz dense wave,and 5 V intensity for 20 minutes. Rats in the Ren/Du meridian + PD98059 group were treated with 0.2 μg PD98059 injection into the subventricular zone,2 μL per rat. Rats in the model group were not treated with electro-acupuncture. MAIN OUTCOME MEASURES:BrdU/nestin immunofluorescent staining was used to detect proliferating neural stem cells in the subventricular zone of cerebral ischemia rats; Western blot was used to determine phosphorylated ERK1 and 2 (pERK1/2) expression in the subventricular zone. RESULTS:On days 14 and 28 after cerebral ischemia,there were significantly more BrdU-positive and BrdU/nestin-positive cells in the Ren /Du meridian group compared with the Du meridian group (P < 0.05). PD98059 decreased the number of BrdU-positive and BrdU/nestin-positive cells induced by electro-acupuncture at the Ren and Du meridians (P < 0.05). On days 7,14,and 28 after treatment,pERK1/2 expression was significantly greater in the Du meridian and Ren/Du meridian groups compared with the model group (P < 0.05). The promoting effect of electro-acupuncture at Ren and Du meridians on ERK1/2 phosphorylation was superior to electro-acupuncture at the Du meridian alone on day 14 after model induction (P < 0.05). However,PD98059 completely abolished the promoting effect of electro-acupuncture at Ren/Du meridians on pERK1/2 expression (P < 0.05). CONCLUSION:Electro-acupuncture at Ren and Du meridians increased proliferation of subventricular zone neural stem cells,which was related to activation of the ERK pathway in a rat model of cerebral ischemia injury. | Wenshu Luo Haibo Yu Zhuoxin Yang Min Pi Lihong Diao Xiaodan Rao | 2010 | Neural Regeneration Research2010，5，6： | 14 |
| 11 | Current understanding of ovarian aging显示文摘The reproductive system of human female exhibits a much faster rate of aging than other body systems.Ovarian aging is thought to be dominated by a gradual decreasing numbers of follicles,coinciding with diminished quality of oocytes.Menopause is the final step in the process of ovarian aging.This review focuses on the mechanisms underlying the ovarian aging involving a poor complement of follicles at birth and a high rate of attrition each month,as well as the alternated endocrine factors.We also discuss the possible causative factors that contribute to ovarian aging,e.g.,genetic factors,accumulation of irreparable damage of microenvironment,pathological effect and other factors.The appropriate and reliable methods to assess ovarian aging,such as quantification of follicles,endocrine measurement and genetic testing have also been discussed.Increased knowledge of the ovarian aging mechanisms may improve the prevention of premature ovarian failure. | LI Qian GENG XiaoDan ZHENG Wei TANG Jie XU Bo SHI QingHua | 2012 | Science China(Life Sciences)2012，55，8： | 14 |
| 12 | Properties of Functionalized Graphene/Room Temperature Vulcanized Silicone Rubber Composites Prepared by an In-situ Reduction Method显示文摘Functionalized graphene oxide (FGO) was prepared by treating graphene oxide with γ-aminopropyl triethoxysilane (KH-550) before the mixture was dispersed into α, ω-dihydroxy polydimethylsiloxane to get room temperature vulcanized (RTV) silicone rubber composites by solution casting. The cured composites were then reduced with hydrazine hydrate to obtain functionalized graphene (FG)/RTV silicone rubber composites. The structures of FGO and the resultant composites were characterized by atomic force microscopy, FT-IR spectra and X-ray diffraction. KH-550 was found to be grafted onto graphene sheets, leading to an increased interlayer spacing. Significant improvements in thermal and mechanical properties were obtained. Both the FGO/RTV silicone rubber composite contain 1.0 wt% of FGO, and its reduced product showed an increase of one-step weight loss temperature with 61 ℃ and 133 ℃ higher than that of pure silicone rubber. Tensile strength and elongation at break of FG/RTV silicone rubber composite (with 0.5 wt% FGO content) increased by 175% and 67%, respectively, compared with those of pure silicone rubber. | 马文石 LI Ji DENG Bangjun LIN Xiaodan ZHAO Xusheng | 2013 | Journal of Wuhan University of Technology(Materials Science)2013，28，1： | 14 |
| 13 | Mutational Analysis of Region-cytotoxicity Relationship in Human Transmembrane Tumor Necrosis Factor-alpha显示文摘Objective:To determine the region of human transmembrane tumor necrosis factor-alpha (TM-TNFa), essential for cytotoxic activity a-gainst human breast cancer cell line MCF-7. Methods:Single amino-acid-substituted TM-TNFα mutant proteins (muteins) were produced by in vitro transcription linked translation techniques. The cDNA of TM-TNFα was site-directed mutagenized by recombinant PCR. Results:13 single amino-acid substituted TM-TNFα muteins were generated and assayed for cytotoxic activity. The cytotoxic activities of TM-TNFα muteins, eg, TM-TNFα-71/Lys, -28/Phe and 117/Leu were significantly decreased (P<0.01) compared to that of parent TM-TNFα, 143/Tyr decreased 4-folds, and-17/Thr,-39/Ser,ll9/His,35/Gly,95/Cys and 147/Phe decreased 1.5-2.5-folds, respectively. However, the cytotoxic activities of TM-TNFα-8/Arg, 31/Gly and 87/Phe showed no significant change. Conclusion:These results indicate that the regions associated with cytotoxic-activity of TM-TNFα are different with that of secretory TNF-lpha (S-TNFα). The inner cell region and transmembrane region of TM-TNFα are related to the cytotoxic activity of TM-TNFα. | ZHENG Fang, GONG Feili, LI Zhuoya, JIANG Xiaodan, XIONG Ping, FENG Wei, XU Yong, Institute of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | 2002 | The Chinese-German Journal of Clinical Oncology2002，1，1： | 14 |
| 14 | Picroside Ⅱ down-regulates matrix metalloproteinase-9 expression following cerebral ischemia/reperfusion injury in rats显示文摘Studies have shown that Picroside II attenuates inflammatory reactions following brain ischemia through the inhibition of the TLR-4-NF-κB signal transduction pathway,and ameliorates cerebral edema through the reduction of aquaporin-4 expression.Matrix metalloproteinase-9 (MMP-9),located downstream of the TLR-4-NF-κB signal transduction pathway,can degrade the neurovascular matrix,damage the blood-brain barrier to induce cerebral edema,and directly result in neuronal apoptosis and brain injury.Therefore,the present study further observed MMP-9 expression in the brain tissues of rats with cerebral ischemia/reperfusion injury following Picroside II treatment.Results demonstrated that Picroside II significantly reduced MMP-9 expression in ischemic brain tissues,as well as neuronal apoptosis and brain infarct volume,suggesting Picroside II exhibits neuroprotection by down-regulating MMP-9 expression and inhibiting cell apoptosis. | Xiang Li Xinying Xu Zhen Li Yunliang Guo Qin Li Xiaodan Li Zhen Zhou | 2010 | Neural Regeneration Research2010，5，18： | 13 |
| 15 | Mechanism of floral scent production in Osmanthus fragrans and the production and regulation of its key floral constituents,β-ionone and linalool显示文摘Sweet osmanthus(Osmanthus fragrans Lour.)is among the top ten most well-known flowers in China and is recognized as both an aromatic plant and ornamental flower.Here,manual sectioning,scanning electron microscopy,and transmission electron microscopy of sweet osmanthus petals revealed that large amounts of lipids are present inside the petal cells and on the cell surfaces.However,no secretory structures were observed.Instead,the petal cells protrude slightly outward,and the surfaces of the cells are adorned with highly regular brush-shaped hairs.The surfaces of the‘Yingui’petals possessed mostly curled and more numerous hairs,whereas the‘Dangui’petals possessed fewer brush-shaped and more sparsely arranged hairs.In addition,many granular substances were attached to the brush-shaped hairs,and the granules were denser on the hairs of the‘Yingui’petals compared to the hairs on the‘Dangui’petals.Furthermore,35 aromatic components in the‘Yingui’petals and 30 aromatic components in the‘Dangui’petals were detected via GC-MS.The main aromatic component of the‘Yingui’petals wasβ-ionone,whereas that of the‘Dangui’petals was linalool and its oxides.Transcriptome sequencing and qRT-PCR indicated that the highβ-ionone content in the‘Yingui’petals was due to the overexpression of CCD1 and CCD4 and that the high linalool content in the‘Dangui’petals was due to the overexpression of MECS,HDR,IDI1,and LIS1,which function upstream of the linalool synthetic pathway.In particular,the expression levels of CCD4 and LIS1 were upregulated by 5.5-and 5.1-fold in the‘Yingui’and‘Dangui’petals,respectively.One transcription factor(ERF61)was cloned and named,and the expression pattern of ERF61 in sweet osmanthus petals was found to be generally consistent with that of CCD4.Tobacco transformation experiments,yeast one-hybrid experiments,and electrophoretic mobility shift assays indicated that ERF61 binds to the CCD4 promoter and stimulates CCD4 expression,thereby regulating the synthesis ofβ-ionone in sweet osmanthus petals. | Yuanji Han Hongyun Wang Xiaodan Wang Ke Li Meifang Dong Yong Li Qian Zhu Fude Shang | 2019 | Horticulture Research2019，6，1： | 13 |
| 16 | Species-dependent neuropathology in transgenic SOD' aigs显示文摘 | Huaqiang Yang Guohao Wang Haitao Sun Runzhe Shu Tao Liu Chuan-En Wang Zhaoming Liu Yu Zhao Bentian Zhao Zhen Ouyang Dongshan Yang Jiao Huang Yueling Zhou Shihua Li Xiaodan Jiang Zhicheng Xiao Xiao-Jiang Li Liangxue Lai | 2014 | Cell Research2014，24，4： | 13 |
| 17 | A novel colloidal gold immunochromatography assay strip for the diagnosis of schistosomiasis japonica in domestic animals显示文摘Background:Schistosomiasis remains a major public health concern in China and an epidemiological survey has revealed that schistosome-infected bovines and goats are the main transmission sources for the disease.Therefore,development of a sensitive technique for the diagnosis of schistosomiasis in domestic animals is necessary.Method:A novel colloidal gold immunochromatography assay(GICA)strip was developed for detecting Schistosoma japonicum in domestic animals.The colloidal gold was conjugated with recombinant streptococcal protein G(rSPG).As the test and control lines,the schistosome soluble egg antigen and rSPG,respectively,were blotted on nitrocellulose membrane.Results:The lowest detectable serum dilution was 1∶640 for schistosome-infected buffaloes.The cross-reaction rate of GICA was 14.29%with Paramphistomum sp.in buffaloes,16.67%with Haemonchus sp.in goats,and 33.33%with Orientobilharzia sp.in goats.These results were slightly lower and similar to those obtained through ELISA.Moreover,the strips for detecting S.japonicum in mice,rabbits,buffaloes,and goats showed high sensitivity(100.00%,100.00%,100.00%,and 100.00%,respectively)and specificity(100.00%,100.00%,94.23%,and 88.64%,respectively).And the sensitivity or specificity of the GICA strips did not present any significant differences after storage for 12 months at room temperature.When compared with ELISA,the GICA strips exhibited similar sensitivity and specificity in the diagnosis of schistosomiasis in mice,rabbits,buffaloes,and goats.Besides,only 5μl of serum are required for the test and the detection can be completed within 5 min.Conclusion:This study is the first to develop a GICA strip using gold-rSPG conjugate for the diagnosing of schistosomiasis in domestic animals,and preliminary results showed that the developed strip may be suitable for large-scale screening of schistosomiasis in endemic areas. | Rui Xu Jintao Feng Yang Hong Chao Lv Dengyun Zhao Jiaojiao Lin Ke Lu Hao Li Jinming Liu Xiaodan Cao Tao Wang Jinli Zai Zhaozhe Wang Bingguang Jia Qian Han Chuangang Zhu | 2017 | Infectious Diseases of Poverty2017，6，1： | 12 |
| 18 | Triage for management of cervical high-grade squamous intraepithelial lesion patients with positive margin by conization: a retrospective analysis显示文摘这研究的目的是为颈的高级有鳞的 intraepithelial 的管理指导 triage 有由 conization 的积极边缘的损害(HSIL ) 病人。有由 conization 的积极边缘的 HSIL 病人的 Clinico 病理学的数据回顾地从 2009 年 1 月被收集到 2014 年 12 月。所有病人在 6 个月以内经历了第二等的 conization 或子宫切除。剩余损害的率被计算,并且与剩余损害联系的因素被 univariate 和 multivariate 分析分析。在 119 个病人的一个总数之中, 56 (47.06%) 病人们在他们的随后的外科的标本介绍了剩余 HSIL,包括侵略的颈的癌(3 阶段 IA1 和 1 个阶段 IA2 病人) 的 4 个盒子。Univariate 分析显示出那耐心的年龄 > 35 年(P=0.005 ) , menopausal 时期 > 5 年(P=0.0035 ) ,和多重象限的参与(P=0.001 ) 显著地与剩余疾病被相关;然而, multivariate 分析揭示了那多重象限的参与(P=0.001;或, 3.701;95%CI, 1.4969.154 ) 是为剩余疾病的一个独立风险因素。将近有由 conization 的积极边缘的 HSIL 病人的一半与多重积极边缘在随后的外科的标本,和那些是没有疾病的可以考虑 re-conization 或重新评价。 | Yuya Dou Xiaodan Zhang Yang Li Fenfen Wang Xing Xie Xinyu Wang | 2017 | Frontiers of Medicine2017，11，2： | 12 |
| 19 | Population trends, threats, and conservation recommendations for waterbirds in China显示文摘Background: China is one of the countries with abundant waterbird diversity. Over the past decades, China's waterbirds have suffered increasing threats from direct and indirect human activities. It is important to clarify the population trends of and threats to waterbirds as well as to put forward conservation recommendations.Methods: We collected data of population trends of a total of 260 waterbird species in China from Wetlands International database. We calculated the number of species with increasing, declining, stable, and unknown trends. We collected threatened levels of waterbirds from the Red List of China's Vertebrates(2016), which was compiled according to the IUCN criteria of threatened species. Based on literature review, we refined the major threats to the threatened waterbird species in China.Results: Of the total 260 waterbird species in China, 84 species(32.3%) exhibited declining, 35 species(13.5%) kept stable, and 16 species(6.2%) showed increasing trends. Population trends were unknown for 125 species(48.1%). There was no significant difference in population trends between the migratory(32.4% decline) and resident(31.8% decline) species or among waterbirds distributed exclusively along coasts(28.6% decline), inland(36.6% decline), and both coasts and inland(32.5% decline). A total of 38 species(15.1% of the total) were listed as threatened species and 27 species(10.8% of the total) Near Threatened species. Habitat loss was the major threat to waterbirds, with 32 of the total 38(84.2%) threatened species being affected. In addition, 73.7%(28 species), 71.1%(27 species), and 57.9%(22 species) of the threatened species were affected by human disturbance, environmental pollution, and illegal hunting, respectively.Conclusions: We propose recommendations for waterbird conservation, including(1) strengthening conservation of nature wetlands and restoration of degraded wetlands,(2) enhancing public awareness on waterbird conservation,(3) improving the enforcement of Wildlife Protection Law and cracking down on illegal hunting,(4) carrying out longterm waterbird surveys to clarify population dynamics,(5) restoring populations of highly-threatened species through artificial intervention, and(6) promoting international and regional exchanges and cooperation to share information in waterbirds and their conservation. | Xiaodan Wang Fenliang Kuang Kun Tan Zhijun Ma | 2018 | Avian Research2018，9，2： | 12 |
| 20 | Olfactory ecto-mesenchymal stem cell-derived exosomes ameliorate murine Sjögren’s syndrome by modulating the function of myeloid-derived suppressor cells显示文摘Sjögren’s syndrome(SS)is a systemic autoimmune disease characterized by progressive inflammation and tissue damage in salivary glands and lacrimal glands.Our previous studies showed that myeloid-derived suppressor cells(MDSCs)exhibited impaired immunosuppressive function during disease progression in patients with SS and mice with experimental Sjögren’s syndrome(ESS),but it remains unclear whether restoring the function of MDSCs can effectively ameliorate the development of ESS.In this study,we found that murine olfactory ecto-mesenchymal stem cell-derived exosomes(OE-MSC-Exos)significantly enhanced the suppressive function of MDSCs by upregulating arginase expression and increasing ROS and NO levels.Moreover,treatment with OE-MSC-Exos via intravenous injection markedly attenuated disease progression and restored MDSC function in ESS mice.Mechanistically,OE-MSC-Exo-secreted IL-6 activated the Jak2/Stat3 pathway in MDSCs.In addition,the abundant S100A4 in OE-MSC-Exos acted as a key factor in mediating the endogenous production of IL-6 by MDSCs via TLR4 signaling,indicating an autocrine pathway of MDSC functional modulation by IL-6.Taken together,our results demonstrated that OE-MSC-Exos possess therapeutic potential to attenuate ESS progression by enhancing the immunosuppressive function of MDSCs,possibly constituting a new strategy for the treatment of Sjögren’s syndrome and other autoimmune diseases. | Ke Rui Yue Hong Qiugang Zhu Xiaofei Shi Fan Xiao Hailong Fu Qing Yin Yida Xing Xinfeng Wu Xiaodan Kong Huaxi Xu Jie Tian Shengjun Wang Liwei Lu | 2021 | Cellular & Molecular Immunology2021，18，2： | 12 |
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