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| 1 | Mesenchymal stem cells-derived exosomal microRNAs contribute to wound inflammation显示文摘Clinical and experimental studies have highlighted the significance of inflammation in coordinating wound repair and regeneration.However,it remains challenging to control the inflammatory response and tolerance at systemic levels without causing toxicity to injured tissues.Mesenchymal stem cells(MSCs) possess potent immunomodulatory properties and facilitate tissue repair by releasing exosomes,which generate a suitable microenvironment for inflammatory resolution.Exosomes contain several effective bioactive molecules and act as a cell-cell communication vehicle to influence cellular activities in recipient cells.During this process,the horizontal transfer of exosomal microRNAs(miRNAs) to acceptor cells,where they regulate target gene expression,is of particular interest for understanding the basic biology of inflammation ablation,tissue homeostasis,and development of therapeutic approaches.In this review,we describe a signature of three specific miRNAs(miR-21,miR-146 a,and miR-181) present in human umbilical cord MSC-derived exosomes(MSC-EXO) identified microarray chip analysis and focus on the inflammatory regulatory functions of these immune-related miRNAs.We also discuss the potential mechanisms contributing to the resolution of wound inflammation and tissue healing. | Dongdong Ti Haojie Hao Xiaobing Fu Weidong Han | 2016 | Science China(Life Sciences)2016，59，12： | 24 |
| 2 | Gold nanorod-photosensitizer conjugate with extracellular pH-driven tumor targeting ability for photothermal/photodynamic therapy显示文摘氯 e6-pHLIP <候补选手class=“ a-plus-plus ”> ss -AuNRs,金使nanorod具有感光性结合包含 pH (低)有给予细胞外的 pH 的一张二硫化物契约的插入肽( pHLIP )( pH <候补选手class=“ a-plus-plus ”> e )-driven 肿瘤指向能力,成功地为光力学的 bimodal 和 photothermal 治疗被开发了。在这 bimodal 治疗,氯 e6 (Ce6 ) ,第二代的 photosensitizer (PS ) ,被用于光力学的治疗(太平洋夏季时间) 。金 nanorods (AuNRs ) 作为 Ce6 的 nanocarrier 和 quencher 为 photothermal 治疗(PTT ) 并且也被用作一个过高热代理人。pHLIPss 作为一根驾驶 pHe 的指向探针被设计在低 pH 在癌症房间提高 Ce6 和 AuNRs 的累积。在 Ce6-pHLIP < 潜水艇 class= “ a-plus-plus ” > ss -AuNRs, Ce6 接近并且由 AuNRs 熄灭了,引起小太平洋夏季时间效果。当暴露了到正常生理的 pH 时 7.4, Ce6-pHLIP < 潜水艇 class= “ a-plus-plus ” > ss -AuNRs 泛泛地与房间膜联系。然而,曾经暴露了到酸的 pH 6.2, pHLIP 活跃地插入到房间膜,和 conjugates 是进房间的 translocated。当这发生时, Ce6 由于二硫化物与 AuNRs 分开细胞内部的谷胱甘肽(GSH ) 引起的契约劈开,和汗衫氧在光之上为太平洋夏季时间被生产照耀。作为单个 PTT 代理人,另外, AuNRs 能由提高的浸透和保留(EPR ) 效果在肿瘤提高 PS 的累积。因此由我们的数据显示了当暴露了到酸的 pH 时, Ce6-pHLIP < 潜水艇 class= “ a-plus-plus ” > ss -AuNRs 能为癌症治疗完成 synergistic PTT/PDT bimodality。 | Nannan Wang Zilong Zhao Yifan Lv Huanhuan Fan Huarong Bai Hongmin Meng Yuqian Long Ting Fu Xiaobing Zhang Weihong Tan | 2014 | Nano Research2014，7，9： | 11 |
| 3 | Genetic engineering of T cells with chimeric antigen receptors for hematological malignancy immunotherapy显示文摘The host immune system plays an instrumental role in the surveillance and elimination of tumors by recognizing and destroying cancer cells. In recent decades, studies have mainly focused on adoptive immunotherapy using engineered T cells for the treatment of malignant diseases. Through gene engraftment of the patient's own T cells with chimeric antigen receptor(CAR),they can recognize tumor specific antigens effectively and eradicate selectively targeted cells in an MHC-independent fashion.To date, CAR-T cell therapy has shown great clinical utility in patients with B-cell leukemias. Owing to different CAR designs and tumor complex microenvironments, genetically redirected T cells may generate diverse biological properties and thereby impact their long-term clinical performance and outcome. Meanwhile some unexpected toxicities that result from CAR-T cell application have been examined and limited the curative effects. Diverse important parameters are closely related with adoptively transferred cell behaviors, including CAR-T cells homing, CAR constitutive signaling, T cell differentiation and exhaustion. Thus, understanding CARs molecular design to improve infused cell efficacy and safety is crucial to clinicians and patients who are considering this novel cancer therapeutics. In this review, the developments in CAR-T cell therapy and the limitations and perspectives in optimizing this technology towards clinical application are discussed. | Dongdong Ti Yunfei Niu Zhiqiang Wu Xiaobing Fu Weidong Han | 2018 | Science China(Life Sciences)2018，61，11： | 10 |
| 4 | Stem cell therapies and regenerative medicine in China显示文摘Stem cells are the core of tissue repair and regeneration,and a promising cell source for novel therapies.In recent years,research into stem cell therapies has been particularly exciting in China.The remarkable advancements in basic stem cell research and clinically effective trials have led to fresh insights into regenerative medicine,such as treatments for sweat gland injury after burns,diabetes,and liver injury.High hopes have inspired numerous experimental and clinical trials.At the same time,government investment and policy support of research continues to increase markedly.However,numerous challenges must be overcome before novel stem cell therapies can achieve meaningful clinical outcomes. | HUANG Sha FU XiaoBing | 2014 | Science China(Life Sciences)2014，57，2： | 10 |
| 5 | TNF-α suppresses sweat gland differentiation of MSCs by reducing FTO-mediated m^6 A-demethylation of Nanog mRNA显示文摘An effect of inhibition of tumor necrosis factor-α(TNF-α)on differentiation of mesenchymal stromal cells(MSCs)has been demonstrated,but the exact mechanisms that govern MSCs differentiation remain to be further elucidated.Here,we show that TNF-αinhibits the differentiation of MSCs to sweat glands in a specific sweat gland-inducing environment,accompanied with reduced expression of Nanog,a core pluripotency factor.We elucidated that fat mass and obesity-associated protein(FTO)-mediated m^6 A demethylation is involved in the regulation of MSCs differentiation potential.Exposure of MSCs to TNF-αreduced expression of FTO,which demethylated Nanog m RNA.Reduced expression of FTO increased Nanog m RNA methylation,decreased Nanog m RNA and protein expression,and significantly inhibited MSCs capacity for differentiation to sweat gland cells.Our finding is the first to elucidate the functional importance of m^6 A modification in MSCs,providing new insights that the microenvironment can regulate the multipotency of MSCs at the post-transcriptional level.Moreover,to maintain differentiation capacity of MSCs by regulating m^6 A modification suggested a novel potential therapeutic target for stem cellmediated regenerative medicine. | Yihui Wang Rui Wang Bin Yao Tian Hu Zhao Li Yufan Liu Xiaoli Cui Liuhanghang Cheng Wei Song Sha Huang Xiaobing Fu | 2020 | Science China(Life Sciences)2020，63，1： | 9 |
| 6 | Percent free prostate-specific antigen for prostate cancer diagnosis in Chinese men with a PSA of 4.0-10.0 ng/mL:Results from the Chinese Prostate Cancer Consortium显示文摘Objective:To test the diagnostic performance of percent free prostate-specific antigen(%fPSA)in predicting any prostate cancer(PCa)and high-grade prostate cancer(HGPCa)in a retrospective multi-center biopsy cohort with a PSA level of 4.0e10.0 ng/mL in China.Methods:Consecutive patients with a PSA of 4.0-10.0 ng/mL who underwent transrectal ultrasound-guided biopsy were enrolled at 16 Chinese medical centers from January 1st,2010 to December 31st,2013.Total and free serum PSA determinations were performed using three types of electro-chemiluminescence immunoassays recalibrated to the World Health Organization(WHO)standard.The diagnostic accuracy of PSA,%fPSA,and %fPSA in combination with PSA(%fPSA t PSA)was determined using the area under the receiver operating characteristic(ROC)curve(AUC).Results:A total of 2310 consecutive men with PSA levels between 4.0 and 10.0 ng/mL were included,and the detection rate of PCa was 25.1%.The AUC of%fPSA and %fPSA t PSA in predicting any PCa was superior to PSA alone in men aged≥60 years(0.623 vs.0.534,p<0.0001)but not in men aged 40e59 years(0.517 vs.0.518,p=0.939).Similar result was yield in predicting HGPCa.Conclusion:In a clinical setting of Chinese men with 4.0e10.0 ng/mL PSA undergoing initial prostate biopsy,adding %fPSA to PSA can moderately improve the diagnostic accuracy for any PCa and HGPCa compared with PSA alone in patients≥60 but not in patients aged 40-59 years. | Rui Chen Liping Xie Xiaobing Cai Yiran Huang Liqun Zhou Lulin Ma Xu Gao Chuanliang Xu Shancheng Ren Pengfei Shao Danfeng Xu Kexin Xu Zhangqun Ye Chunxiao Liu Dingwei Ye Li Lu Qiang Fu Jianquan Hou Jianlin Yuan Dalin He Tie Zhou Fubo Wang Biming He Yinghao Sun | 2015 | Asian Journal of Urology2015，2，2： | 8 |
| 7 | Chemical conversion of human and mouse fibroblasts into motor neurons显示文摘Transplantation of motor neurons can provide long-term functional benefits in animal models of neurodegenerative motor neuron diseases such as amyotrophic lateral sclerosis and traumatic spinal cord injury. Although embryonic stem cells can differentiate into motor neurons, alternative sources of motor neurons may be controllable for disease modeling and transplantation. Here, we show that human and mouse fibroblasts can be efficiently and directly converted into motor neurons by a cocktail of five small molecules, without the involvement of the neural progenitor stage. The chemically-induced motor neurons display the distinct neuronal morphology and express motor neuron markers. Interestingly, when the same chemical compounds were soaked in beads and implanted in the hypodermis of the back skins of mice, surrounding cells begin to express motor neuron markers,indicating in vivo motor neuron reprogramming. Taken together, we provide an efficient approach for chemically converting human and mouse fibroblasts into motor neurons suitable for cell replacement therapy and neurodegenerative disease modeling. | Hua Qin ANDong Zhao Kui Ma Xiaobing Fu | 2018 | Science China(Life Sciences)2018，61，10： | 5 |
| 8 | Growth factor regulatory system:a new system for not truly recognized organisms显示文摘Growth,development,aging,and disease occurrence in organisms,especially animals or Homo sapiens,are regulated by different regulatory systems in vivo.The nervous,endocrine,metabolic,and immune systems are the traditional biological systems essential for the growth,development,and metabolism of the body.These systems maintain a balance and precisely biologically regulate every aspect of life activities under normal circumstances.However,in abnormal situations,owing to endogenous or exogenous attack,these regulatory systems result in dysfunction,subsequently leading to illness or injury(Muggianu et al.,2018).In the classical neuroendocrine regulatory system,Cushing’s syndrome is defined as a series of abnormal clinical symptoms with chronic overproduction of glucocorticoids in the adrenal cortex.Its clinical manifestations are“moon face”and“buffalo hump,”involving blood circulation,bone metabolism,and gonadal function,among others.In addition,severe trauma or burn leads to an imbalanced immune regulatory system,resulting in excessive inflammatory reactions and sepsis.Thus,an independent or cooperative regulation of multiple regulatory systems is critical in maintaining the body’s homeostasis,healthy development,and growth. | Jian Xiao Zhuofeng Lin Hua Qin Zhilong Zheng Fanghua Gong Yanlong Liu Xiaokun Li Xiaobing Fu | 2020 | Science China(Life Sciences)2020，63，3： | 5 |
| 9 | Linear Strain-Gradient Effect on the Energy Bandgap in Bent CdS Nanowires显示文摘尽管在半导体的可能的非同类的紧张效果被调查了因为在一半上世纪和紧张坡度能每在灵活 nanostructures 的测微计是超过 1% ,我们仍然缺乏他们对精力乐队的影响的理解。这里,我们进行紧张坡度的系统的阴极射线发光光谱学研究在在低温度的有弹性地弄弯的 CdS nanowires 的导致的激子精力移动,并且发现在弄弯的 nanowires 的激子精力红移动与紧张坡度成正比,格子失真的一个索引。密度功能的计算在弄弯的 nanostructures 显示出乐队差距减小的一样的趋势并且揭示内在的机制。半导体的乐队差距上的重要线性紧张坡度效果应该在在 nanoelectronics 调节光电子的性质的方法上打开新灯。 | Qiang Fu Zi Yue Zhang Liangzhi KOU Peicai Wu Xiaobing Han Xinli Zhu Jingyun Gao Jun Xu Qing Zhao Wanlin Guo Dapeng Yu | 2011 | Nano Research2011，4，3： | 5 |
| 10 | Wound healing center establishment and new technology application in improving the wound healing quality in China显示文摘Wound healing,tissue repair and regenerative medicine are in great demand,and great achievements in these fields have been made.In recent years,many of these successes have benefitted patients,especially in the field of chronic skin wounds.However,perfect tissue repair and regeneration of damaged tissues and organs are still great challenges in the management of trauma and diseases.In this paper,the main achievements in wound healing,tissue repair and regeneration in China are reviewed and the establishment of wound healing centers and new technology application in improving wound healing quality in patients in China is highlighted. | Xiaobing Fu | 2020 | Burns & Trauma2020，8，1： | 5 |
| 11 | Soil Nutrient Variance by Slope Position in a Mollisol Farmland Area of Northeast China显示文摘In order to generate scientifically-based comparative information to improve fertilization efficiency and reduce nutrient loss, 610 samples of 122 soil profiles were collected at the 0–60 cm depth to compare soil nutrient contents including soil organic matter(SOM), total nitrogen(TN), total phosphorus(TP), available phosphorus(AP), and available potassium(AK) among different slope positions in a Mollisol farmland area of Northeast China. The contents of SOM and TN typically decreased with increased soil depth at back and bottom slope. Soil loss and deposition tended to decrease SOM and TN at the 0–20 cm soil depth on both the back slope and the slope bottom. The TP firstly decreased from 0–20 cm to 30–40 cm, and then not constantly increased at the back slope and the bottom slope. Due to the characteristics of soil nutrients and crop absorption, the contents of both AP and AK were typically the highest at the summit, followed by the slope bottom and the back slope in the 0–20 cm layer. Generally, in order to sustain the high soil productivity and protect the environment, attention should be paid to soil conservation on back slope; in addition, additional N and P fertilizer is necessary on the back slope. | ZHANG Shaoliang JIANG Lili LIU Xiaobing ZHANG Xingyi FU Shicong DAI Lin | 2016 | Chinese Geographical Science2016，26，4： | 5 |
| 12 | Redirecting differentiation of mammary progenitor cells by 3D bioprinted sweat gland microenvironment显示文摘Background:Mammary progenitor cells(MPCs)maintain their reproductive potency through life,and their specific microenvironments exert a deterministic control over these cells.MPCs provides one kind of ideal tools for studying engineered microenvironmental influence because of its accessibility and continually undergoes postnatal developmental changes.The aim of our study is to explore the critical role of the engineered sweat gland(SG)microenvironment in reprogramming MPCs into functional SG cells.Methods:We have utilized a three-dimensional(3D)SG microenvironment composed of gelatin-alginate hydrogels and components from mouse SG extracellular matrix(SG-ECM)proteins to reroute the differentiation of MPCs to study the functions of this microenvironment.MPCs were encapsulated into the artificial SG microenvironment and were printed into a 3D cell-laden construct.The expression of specific markers at the protein and gene levels was detected after cultured 14 days.Results:Compared with the control group,immunofluorescence and gene expression assay demonstrated that MPCs encapsulated in the bioprinted 3D-SG microenvironment could significantly express the functional marker of mouse SG,sodium/potassium channel protein ATP1a1,and tend to express the specific marker of luminal epithelial cells,keratin-8.When the Shh pathway is inhibited,the expression of SG-associated proteins in MPCs under the same induction environment is significantly reduced.Conclusions:Our evidence proved the ability of differentiated mouse MPCs to regenerate SG cells by engineered SG microenvironment in vitro and Shh pathway was found to be correlated with the changes in the differentiation.These results provide insights into regeneration of damaged SG by MPCs and the role of the engineered microenvironment in reprogramming cell fate. | Rui Wang Yihui Wang Bin Yao Tian Hu Zhao Li Yufan Liu Xiaoli Cui Liuhanghang Cheng Wei Song Sha Huang Xiaobing Fu | 2019 | Burns & Trauma2019，7，1： | 5 |
| 13 | A widely adaptable approach to generate integration-free iPSCs from non-invasively acquired human somatic cells显示文摘 | Zhichao Ding Lina Sui Ruotong Ren Yanjun Liu Xiuling Xu Lina Fu Ruijun Bai Tingting Yuan Ying Hao Weiqi Zhang Huize Pan Wensu Liu Han Yu Concepcion Rodriguez Esteban Xiaobing Yu Ze Yang Jian Li Xiaomin Wang Juan Carlos Izpisua Belmonte Guang-Hui Liu Fei Yi Jing Qu | 2015 | Protein & Cell2015，6，5： | 5 |
| 14 | Microenvironment-induced myofibroblast-like conversion of engrafted keratinocytes显示文摘Myofibroblasts,recognized classically by-smooth muscle actin(-SMA)expression,play a key role in the wound-healing process,promoting wound closure and matrix deposition.Although a body of evidence shows that keratinocytes explanted onto a wound bed promote closure of a skin injury,the underlying mechanisms are not well understood.The basal layer of epidermis is rich in undifferentiated keratinocytes(UKs).We showed that UKs injected into granulation tissue could switch into-SMA positive cells,and accelerate the rate of skin wound healing.In addition,when the epidermis sheets isolated from foreskin cover up the wound bed or are induced in vitro,keratinocytes located at the basal layers or adjacent sites were observed to convert into myofibroblast-like cells.Thus,UKs have a potential for myofibroblastic transition,which provides a novel mechanism by which keratinocyte explants accelerate skin wound healing. | LI MeiRong TI DongDong HAN WeiDong FU XiaoBing | 2014 | Science China(Life Sciences)2014，57，2： | 4 |
| 15 | The role of the microenvironment on the fate of adult stem cells显示文摘Adult stem cells(SCs) exist in all tissues that promote tissue growth, regeneration, and healing throughout life. The SC niche in which they reside provides signals that direct them to proliferate, differentiate, or remain dormant; these factors include neighboring cells, the extracellular matrix, soluble molecules, and physical stimuli. In disease and aging states, stable or transitory changes in the microenvironment can directly cause SC activation or inhibition in tissue healing as well as functional regulation. Here, we discuss the microenvironmental regulation of the behavior of SC and focus on plasticity approaches by which various environmental factors can enhance the function of SCs and more effectively direct the fate of SCs. | DONG Liang HAO HaoJie HAN WeiDong FU XiaoBing | 2015 | Science China(Life Sciences)2015，58，7： | 4 |
| 16 | Regenerative medicine in China: new advances and hopes显示文摘Tissue and organ repair as well as regeneration are globalfrontiers in the field of stem cell research and regenerativemedicine. Regenerative medicine is closely associated withtreatments of almost all human diseases, injuries, and ag-ing-related problems. The rapid advancements in stem celltechnology, tissue engineering, and physical as well aschemical interventions have resulted in significant progressin tissue and organ repair as well as regeneration. Currently,a single tissue type can be repaired and regenerated throughcell transplantation, trophic factors, biodegradable scaffolds, | Xiaobing Fu | 2018 | Science China(Life Sciences)2018，61，10： | 4 |
| 17 | Chemical modulation of cell fates: in situ regeneration显示文摘Chemical modulation of cell fates has been widely used to promote tissue and organ regeneration. Small molecules can target the self-renewal, expansion, differentiation, and survival of endogenous stem cells for enhancing their regenerative power or induce dedifferentiation or transdifferentiation of mature cells into proliferative progenitors or specialized cell types needed for regeneration. Here, we discuss current progress and potential using small molecules to promote in vivo regenerative processes by regulating the cell fate. Current studies of small molecules in regeneration will provide insights into developing safe and efficient chemical approaches for in situ tissue repair and regeneration. | Hua Qin Andong Zhao Xiaobing Fu | 2018 | Science China(Life Sciences)2018，61，10： | 4 |
| 18 | Progress of Jinping Underground laboratory for Nuclear Astrophysics(JUNA)显示文摘Jinping Underground laboratory for Nuclear Astrophysics(JUNA) will take the advantage of the ultra-low background of CJPL lab and high current accelerator based on an ECR source and a highly sensitive detector to directly study for the first time a number of crucial reactions occurring at their relevant stellar energies during the evolution of hydrostatic stars. In its first phase, JUNA aims at the direct measurements of^(25)Mg(p,γ)^(26)Al,^(19)F(p,α)^(16)O,^(13)C(α,n)^(16)O and ^(12)C(α,γ)^(16)O reactions. The experimental setup,which includes an accelerator system with high stability and high intensity, a detector system, and a shielding material with low background, will be established during the above research. The current progress of JUNA will be given. | WeiPing Liu ZhiHong Li JiangJun He XiaoDong Tang Gang Lian Zhu An JianJun Chang Han Chen QingHao Chen XiongJun Chen ZhiJun Chen BaoQun Cui XianChao Du ChangBo Fu Lin Gan Bing Guo GuoZhu He Alexander Heger SuQing Hou HanXiong Huang Ning Huang BaoLu Jia LiYang Jiang Shigeru Kubono JianMin Li KuoAng Li Tao Li YunJu Li Maria Lugaro XiaoBing Luo HongYi Ma ShaoBo Ma DongMing Mei YongZhong Qian JiuChang Qin Jie Ren YangPing Shen Jun Su LiangTing Sun WanPeng Tan Isao Tanihata Shuo Wang Peng Wang YouBao Wang Qi Wu ShiWei Xu ShengQuan Yan LiTao Yang Yao Yang XiangQing Yu Qian Yue Sheng Zeng HuanYu Zhang Hui Zhang LiYong Zhang NingTao Zhang QiWei Zhang Tao Zhang XiaoPeng Zhang XueZhen Zhang ZiMing Zhang Wei Zhao Zuo Zhao Chao Zhou | 2016 | Science China(Physics,Mechanics & Astronomy)2016，59，4： | 3 |
| 19 | 3D-printable supramolecular hydrogels with shear-thinning property: fabricating strength tunable bioink via dual crosslinking显示文摘3-dimensional(3D)bioprinting technology provides promising strategy in the fabrication of artificial tissues and organs.As the fundamental element in bioprinting process,preparation of bioink with ideal mechanical properties without sacrifice of biocompatibility is a great challenge.In this study,a supramolecular hydrogel-based bioink is prepared by polyethylene glycol(PEG)grafted chitosan,α-cyclodextrin(α-CD)and gelatin.It has a primary crosslinking structure through the aggregation of the pseudo-polyrotaxane-like side chains,which are formed from the host-guest interactions betweenα-CD and PEG side chain.Apparent viscosity measurement shows the shear-shinning property of this bioink,which might be due to the reversibility of the physical crosslinking.Moreover,withβ-glycerophosphate at different concentrations as the secondary crosslinking agent,the printed constructs demonstrate different Young's modulus(p<0.001).They could also maintain the Young's modulus in cell culture condition for at least 21 days(p<0.05).By co-culturing each component with fibroblasts,CCK-8 assay demonstrate cellular viability is higher than 80%.After bioprinting and culturing,immunofluorescence staining with quantification indicate the expression of Ki-67,Paxillin,and N-cadherin is higher in day 14 than those in day 3(p<0.05).Oil red O and Nissl body specific staining reflect strength tunable bioink may have impact on the cell fate of mesenchymal stem cells(p<0.05).This work might provide new idea for advanced bioink in the application of re-establishing complicated tissues and organs. | Tian Hu Xiaoliang Cui Meng Zhu Man Wu Ye Tian Bin Yao Wei Song Zhongwei Niu Sha Huang Xiaobing Fu | 2020 | Bioactive Materials2020，5，4： | 3 |
| 20 | Calcium silicate accelerates cutaneous wound healing with enhanced re-epithelialization through EGF/EGFR/ERK-mediated promotion of epidermal stem cell functions显示文摘Background:Human epidermal stem cells(hESCs)play an important role in re-epithelialization and thereby in facilitating wound healing,while an effective way to activate hESCs remains to be explored.Calcium silicate(CS)is a form of bioceramic that can alter cell behavior and promote tissue regeneration.Here,we have observed the effect of CS on hESCs and investigated its possible mechanism.Methods:Using a mouse full-thickness skin excision model,we explored the therapeutic effect of CS on wound healing and re-epithelialization.In vitro,hESCs were cultured with diluted CS ion extracts(CSIEs),and the proliferation,migration ability and stemness of hESCs were evaluated.The effects of CS on the epidermal growth factor(EGF),epidermal growth factor receptor(EGFR)and extracellular signal-related kinase(ERK)signaling pathway were also explored.Results:In vivo,CS accelerated wound healing and re-epithelialization.Immunohistochemistry demonstrated that CS upregulated cytokeratin 19 and integrinβ1 expression,indicating that CS improved hESCs stemness.In vitro studies confirmed that CS improved the biological function of hESCs.And the possible mechanism could be due to the activation of the EGF/EGFR/ERK signaling pathway.Conclusion:CS can promote re-epithelialization and improve the biological functions of hESCs via activating the EGF/EGFR/ERK signaling pathway. | Bingmin Li Haowen Tang Xiaowei Bian Kui Ma Jiang Chang Xiaobing Fu Cuiping Zhang | 2021 | Burns & Trauma2021，9，1： | 3 |
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