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| 1 | Structural basis of AMPK regulation by adenine nucleotides and glycogen显示文摘激活安培的蛋白质 kinase (AMPK ) 为糖尿病,肥胖,和癌症的治疗是一个中央细胞的精力传感器和精力动态平衡,和一个有希望的药目标的管理者。这里我们人的 α 的现在的低分辨率的水晶结构; 1 β 2 γ 1 holo-AMPK 建筑群跳了到它的 allosteric 调节的人安培和肝糖模仿 cyclodextrin,两个在 phosphorylated (4.05 Å) 并且 non-phosphorylated (4.60 Å) 状态。另外,我们解决了 2.95 Å人的 kinase 领域(KD ) 的结构跳了到邻近的 autoinhibitory 领域(帮助) 并且表现了广泛生物化学并且 mutational 研究。一起,这些研究由安培和肝糖说明 allosteric AMPK 调整的内在的机制,其绑定改变在交替的帮助(安培) 和糖类绑定模块(肝糖) 之间的 equilibria 相互作用。 | Xiaodan Li Lili Wang X Edward Zhou Jiyuan Ke Parker W de Waal Xin Gu M H Eileen Tan Dongye Wang Donghai Wu H Eric Xu Karsten Melcher | 2015 | Cell Research2015，25，1： | 8 |
| 2 | Destabilization of strigolactone receptor DWARF14 by binding of ligand and E3-1igase signaling effector DWARF3显示文摘 | Li-Hua Zhao X Edward Zhou Wei Yi Zhongshan Wu Yue Liu Yanyong Kang Li Hou Parker W de Waal Suling Li Yi Jiang Adrian Scaffidi Gavin R Flematti Steven M Smith Vinh Q Lam Patrick R Griffin YonghongWang Jiayang Li Karsten Melcher H Eric Xu | 2015 | Cell Research2015，25，11： | 7 |
| 3 | A complex structure of arrestin-2 bound to a G protein-coupled receptor显示文摘Arrestins comprise a family of signal regulators of G-protein-coupled receptors(GPCRs),which include arrestins 1 to 4.While arrestins 1 and 4 are visual arrestins dedicated to rhodopsin,arrestins 2 and 3(Arr2 and Arr3)areβ-arrestins known to regulate many nonvisual GPCRs.The dynamic and promiscuous coupling of Arr2 to nonvisual GPCRs has posed technical challenges to tackle the basis of arrestin binding to GPCRs.Here we report the structure of Arr2 in complex with neurotensin receptor 1(NTSR1),which reveals an overall assembly that is strikingly different from the visual arrestin–rhodopsin complex by a 90°rotation of Arr2 relative to the receptor.In this new configuration,intracellular loop 3(ICL3)and transmembrane helix 6(TM6)of the receptor are oriented toward the N-terminal domain of the arrestin,making it possible for GPCRs that lack the C-terminal tail to couple Arr2 through their ICL3.Molecular dynamics simulation and crosslinking data further support the assembly of the Arr2‒NTSR1 complex.Sequence analysis and homology modeling suggest that the Arr2‒NTSR1 complex structure may provide an alternative template for modeling arrestin–GPCR interactions. | Wanchao Yin Zhihai Li Mingliang Jin Yu-Ling Yin Parker Wde Waal Kuntal Pal Yanting Yin Xiang Gao Yuanzheng He Jing Gao Xiaoxi Wang Yan Zhang Hu Zhou Karsten Melcher Yi Jiang Yao Cong X.Edward Zhou Xuekui Yu H.Eric Xu | 2019 | Cell Research2019，29，12： | 4 |
| 4 | The structural basis of the dominant negative phenotype of the Gαi1β1Y2 G203A/A326S heterotrimer显示文摘 | Ping LIU Ming-zhu JIA X Edward ZHOU Parker W DE WAAL Bradley M DICKSON Bo LlU Li HOU Yan-ting YIN Ya n-yong KANG Yi SHI Karsten MELCHER H Eric XU Yi JIANG | 2016 | Acta Pharmacologica Sinica2016，37，9： | 3 |
| 5 | The role of interleukin-10 in autoimmune disease:systemic lupus erythematosus (SLE) and multiple sclerosis (MS) 显示文摘 | Beebe A M Cua D J de Waal Malefyt R | 2002 | Cytokine Growth Factor Rev2002，13，45： | 2 |
| 6 | 细胞色素c氧化酶可溶性Cu_A结构域的表达、分离纯化和初步表征显示文摘细胞色素c氧化酶亚基Ⅱ含有可溶性双核铜中心 CuA结构域蛋白.报道了 Paracoccus versutus菌CuA结构域的基因克隆、表达和分离纯化及其初步表征.编码CuA结构域的基因插入 pET11d质粒载体中,于E.coli BL21(DE3)中进行表达.结果表明, CuA结构域蛋白在这个表达体系中主要以包涵体的形式存在,表达量占菌体总蛋白的10%左右.经尿素溶解,Cu~+/Cu~2+重组复性,Q-Sepharose快速阴离子交换柱和Sephadex-G75凝胶过滤柱纯化,得到了电泳纯的紫红色可溶性蛋白.紫外-可见吸收光谱在478nm有较强吸收峰, 530nm处有一肩峰,在 360和 806 nm处有弱吸收峰,它们可归属为混价双金属核中心(Cu2S2R2)中Cu-Cu和Cu-Cu间的电荷转移与轨道相互作用.圆二色谱表明其二级结构主要为β-折叠形式.荧光激发谱最大波长为 280nm,发射谱最大波长为 345 nm. | 李连之 宋爱新 谢毅 黄仲贤 E.de Waal U.Kolczak G.W.Canters | 2001 | 科学通报2001，46，12： | 2 |
| 7 | INTERLEUKIN-10 AND THE INTERLEUKIN-10 RECEPTOR显示文摘 | Kevin W. Moore Rene de Waal Malefyt Robert L. Coffman Anne O’Garra | 2001 | Annual Review of Immunology2001，，： | 2 |
| 8 | IL-27 and IFN-α signal via Stat1 and Stat3 and induce T-Bet and IL-12Rβ2 in naive T cells显示文摘 | Hibbert L Pflanz S de Waal Malefyt R | 2003 | J Interferon Cytokine Res2003，23，9： | 1 |
| 9 | Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA 显示文摘 | MUYZER G DE WAAL E C UITTERLINDEN A G | 1993 | Applied and Environmental Microbiology1993，59，3： | 1 |
| 10 | Interleukin 10 (IL - 10) inhibits cytokine synthesis by human monocytes:An autoregulatory role of IL- 10 produced by monocytes显示文摘 | Rene de Waal Malefyt Jhon Abrams Bruce Bennet | 1991 | Exp Med1991，174，11： | 1 |
| 11 | Interleukin 10 (IL-10) inhibits cytokine synthesis by human monocytes: an autoregulatory role of IL-10 produced by monocytes显示文摘 | De Waal Malefyt R Abrams J Bennett B | 1991 | The Journal of Experimental Medicine1991，174，5： | 1 |
| 12 | Profiling of complex microbial populations by denaturing gradient gel polymerase chain reaction amplified genes encoding for 16S rRNA 显示文摘 | Muyzer G de Waal E C Uitterlinden A G | 1993 | Appl Environ Microbiol1993，59，： | 1 |
| 13 | Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA显示文摘 | Muyzer G de Waal E C Uitterlinden A G | | 0，，03： | 1 |
| 14 | Interleukin-l0 and the interleukin-1O receptor显示文摘 | Moore KW de Waal Malefyt R Coffman RL | 2001 | Annu Rev Immunol2001，19，： | 1 |
| 15 | Perioperative statin thera- py in patients at high risk for cardiovascular morbidity undergoing surgery: a review 显示文摘 | de Waal BA Buise MP van Zundert AA | 2015 | Br J Anaesth2015，114，1： | 1 |
| 16 | INTERLEUKIN-10 AND THE INTERLEUKIN-10 RECEPTOR显示文摘 | Kevin W. Moore Rene de Waal Malefyt Robert L. Coffman Anne O’Garra | 2001 | Annual Review of Immunology2001，，： | 1 |
| 17 | Effect of ice compression on pain after mandibular third molar surgery: a single-blind, randomized controlled trial显示文摘 | T. Forouzanfar A. Sabelis S. Ausems J.A. Baart I. van der Waal | 2008 | International Journal of Oral & Maxillofacial Surgery2008，，9： | 1 |
| 18 | The role of vascular permeability factor and basic fibroblast growth factor in tumor angiogenesis显示文摘 | Westphal HR de waal RM | 1995 | Biol Chem Hoppe Seyler1995，376，2： | 1 |
| 19 | Oral metastases:report of 24 cases显示文摘 | Van der waal RI Buter J Van der waal I | 2003 | Br J Oral Maxillofac Surg2003，41，1： | 1 |
| 20 | Linear IgA bullous dermatosis in a patient with renal cell carcinoma显示文摘 | van der Waal RI van de Scheur MR Pas HH | 2001 | Br J Dermatol2001，144，4： | 1 |
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