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| 1 | Control of Leaf Senescence by an MeOH- Jasmonates Cascade that Is Epigenetically Regulated by OsSRT1 in Rice显示文摘 | ChuanYing Fang Hua Zhang Jian Wan YangYang Wu Kang Li Cheng Jin Wei Chen ShouChuang Wang WenSheng Wang HaiWei Zhang Pan Zhang Fei Zhang LiangHuan Qu Xianqing Liu Dao-Xiu Zhou Jie Luo | 2016 | Molecular Plant2016，9，10： | 14 |
| 2 | Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines显示文摘SARS-CoV-2 variants could induce immune escape by mutations on the receptor-binding domain(RBD)and N-terminal domain(NTD).Here we report the humoral immune response to circulating SARS-CoV-2 variants,such as 501Y.V2(B.1.351),of the plasma and neutralizing antibodies(NAbs)elicited by CoronaVac(inactivated vaccine),ZF2001(RBD-subunit vaccine)and natural infection.Among 86 potent NAbs identified by high-throughput single-cell VDJ sequencing of peripheral blood mononuclear cells from vaccinees and convalescents,near half anti-RBD NAbs showed major neutralization reductions against the K417N/E484K/N501Y mutation combination,with E484K being the dominant cause.VH3-53/VH3-66 recurrent antibodies respond differently to RBD variants,and K417N compromises the majority of neutralizing activity through reduced polar contacts with complementarity determining regions.In contrast,the 242–244 deletion(242–244Δ)would abolish most neutralization activity of anti-NTD NAbs by interrupting the conformation of NTD antigenic supersite,indicating a much less diversity of anti-NTD NAbs than anti-RBD NAbs.Plasma of convalescents and CoronaVac vaccinees displayed comparable neutralization reductions against pseudo-and authentic 501Y.V2 variants,mainly caused by E484K/N501Y and 242–244Δ,with the effects being additive.Importantly,RBD-subunit vaccinees exhibit markedly higher tolerance to 501Y.V2 than convalescents,since the elicited anti-RBD NAbs display a high diversity and are unaffected by NTD mutations.Moreover,an extended gap between the third and second doses of ZF2001 leads to better neutralizing activity and tolerance to 501Y.V2 than the standard three-dose administration.Together,these results suggest that the deployment of RBD-vaccines,through a third-dose boost,may be ideal for combating SARS-CoV-2 variants when necessary,especially for those carrying mutations that disrupt the NTD supersite. | Yunlong Cao Ayijiang Yisimayi Yali Bai Weijin Huang Xiaofeng Li Zhiying Zhang Tianjiao Yuan Ran An Jing Wang Tianhe Xiao Shuo Du Wenping Ma Liyang Song Yongzheng Li Xiang Li Weiliang Song Jiajing Wu Shuo Liu Xuemei Li Yonghong Zhang Bin Su Xianghua Guo Yangyang Wei Chuanping Gao Nana Zhang Yifei Zhang Yang Dou Xiaoyu Xu Rui Shi Bai Lu Ronghua Jin Yingmin Ma Chengfeng Qin Youchun Wang Yingmei Feng Junyu Xiao Xiaoliang Sunney Xie | 2021 | Cell Research2021，31，7： | 12 |
| 3 | CPSF30-L-mediated recognition of mRNA m^(6)A modification controls alternative polyadenylation of nitrate signaling-related gene transcripts in Arabidopsis显示文摘N6-methyladenosine(m^(6)A),a ubiquitous internal modification of eukaryotic mRNAs,plays a vital role in almost every aspect of mRNA metabolism.However,there is little evidence documenting the role of m^(6)A in regulating alternative polyadenylation(APA)in plants.APA is controlled by a large protein-RNA complex with many components,including CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR30(CPSF30).In Arabidopsis,CPSF30 has two isoforms and the longer isoform(CPSF30-L)contains a YT512-B Homology(YTH)domain,which is unique to plants.In this study,we showed that CPSF30-L YTH domain binds to m^(6)A in v itro.In the cpsf30-2 mutant,the transcripts of many genes including several important nitrate signaling-related genes had shifts in polyadenylation sites that were correlated with m^(6)A peaks,indicating that these gene transcripts carrying m^(6)A tend to be regulated by APA.Wild-type CPSF30-L could rescue the defects in APA and nitrate metabolism in cpsf30-2,but m^(6)A-binding-defective mutants of CPSF30-L could not.Taken together,our results demonstrated that m^(6)A modification regulates APA in Arabidops is and revealed that the m^(6)A reader CPSF30-L affects nitrate signaling by controlling APA,shedding new light on the roles of the m^(6)A modification during RNA 3-end processing in nitrate metabolism. | Yiteng Hou Jing Sun Baixing Wu Yangyang Gao Hongbo Nie Zhentian Nie Shuxuan Quan Yong Wang Xiaofeng Cao Sisi Li | 2021 | Molecular Plant2021，14，4： | 12 |
| 4 | In vivo self-assembled small RNAs as a new generation of RNAi therapeutics显示文摘RNAi therapy has undergone two stages of development,direct injection of synthetic siRNAs and delivery with artificial vehicles or conjugated ligands;both have not solved the problem of efficient in vivo siRNA delivery.Here,we present a proof-of-principle strategy that reprogrammes host liver with genetic circuits to direct the synthesis and self-assembly of siRNAs into secretory exosomes and facilitate the in vivo delivery of siRNAs through circulating exosomes.By combination of different genetic circuit modules,in vivo assembled siRNAs are systematically distributed to multiple tissues or targeted to specific tissues(e.g.,brain),inducing potent target gene silencing in these tissues.The therapeutic value of our strategy is demonstrated by programmed silencing of critical targets associated with various diseases,including EGFR/KRAS in lung cancer,EGFR/TNC in glioblastoma and PTP1B in obesity.Overall,our strategy represents a next generation RNAi therapeutics,which makes RNAi therapy feasible. | Zheng Fu Xiang Zhang Xinyan Zhou Uzair Ur-Rehman Mengchao Yu Hongwei Liang Hongyuan Guo Xu Guo Yan Kong Yuanyuan Su Yangyang Ye Xiuting Hu Wei Cheng Jinrong Wu Yanbo Wang Yayun Gu Sheng-feng Lu Dianqing Wu Ke Zen Jing Li Chao Yan Chen-Yu Zhang Xi Chen | 2021 | Cell Research2021，31，6： | 10 |
| 5 | High resolution temperature measurement technique for measuring marine heat flow显示文摘High resolution temperature measurement technique is one of the key techniques for measuring marine heat flow. Basing on Pt1000 platinum resistance which has the characteristics of high accuracy and good stability, we designed a bridge reversal excitation circuit for high resolution temperature measurement. And the deep ocean floor in-situ test results show that: (1) temperature deviation and peak-to-peak resolution of the first version circuit board (V1) are 1.960-1.990 mK and 0.980-0.995 m Kat 1.2-2.7°C, respectively; and temperature deviation and peak-to-peak resolution of the second circuit board (V2) are 2.260mK and 1.130 mK at 1.2-1.3°C, respectively; (2) During the 2012NSFC-IndOcean cruise, seafloor geothermal gradient at Ind2012HF03,-07 and-12 stations (water depth ranges from 3841 to 4541 m) were successfully measured, the values are 59.1,75.1 and 71.6°C/km, respectively. And the measurement errors of geothermal gradient at these three stations are less than 3.0% in terms of the peak-to-peak resolution. These indicate that the high resolution temperature measurement technique based on Pt1000 platinum resistance in this paper can be applied to marine heat flow measurement to obtain high precision geothermal parameters. | QIN YangYang YANG XiaoQiu WU BaoZhen SUN ZhaoHua SHI XiaoBin | 2013 | Science China(Technological Sciences)2013，56，7： | 9 |
| 6 | Defects enriched hollow porous Co-N-doped carbons embedded with ultrafine CoFe/Co nanoparticles as bifunctional oxygen electrocatalyst for rechargeable flexible solid zinc-air batteries显示文摘The construction and design of highly efficient and inexpensive bifunctional oxygen electrocatalysts substitute for noble-metal-based catalysts is highly desirable for the development of rechargeable Zn-air battery(ZAB).In this work,a bifunctional oxygen electrocatalysts of based on ultrafine CoFe alloy(4-5 nm)dispersed in defects enriched hollow porous Co-N-doped carbons,made by annealing SiO2 coated zeolitic imidazolate framework-67(ZIF-67)encapsulated Fe ions.The hollow porous structure not only exposed the active sites inside ZIF-67,but also provided efficient charge and mass transfer.The strong synergetic coupling among high-density CoFe alloys and Co-N_(x) sites in Co,N-doped carbon species ensures high oxygen reduction reaction(ORR)and oxygen evolution reaction(OER)activity.First-principles simulations reveal that the synergistic promotion effect between CoFe alloy and Co-N site effectively reduced the formation energy of from O^(*)to OH^(*).The optimized CoFe-Co@PNC exhibits outstanding electrocatalytic stability and activity with the overpotential of only 320 mV for OER at 10 mA·cm^(−2) and the half-wave potential of 0.887 V for ORR,outperforming that of most recent reported bifunctional electrocatalysts.A rechargeable ZAB constructed with CoFe-Co@PNC as the air cathode displays long-term cyclability for over 200 h and high power density(152.8 mW·cm^(−2)).Flexible solid-state ZAB with our CoFe-Co@PNC as the air cathode possesses a high open circuit potential(OCP)up to 1.46 V as well as good bending flexibility.This universal structure design provides an attractive and instructive model for the application of nanomaterials derived from MOF in the field of sustainable flexible energy applications device. | Zhao Lei Yangyang Tan Zeyi Zhang Wei Wu Niancai Cheng Runzhe Chen Shichun Mu Xueliang Sun | 2021 | Nano Research2021，14，3： | 9 |
| 7 | Synthesis and Characterization of Condensed Polynuclear Aromatic Resin Derived from Ethylene Tar显示文摘As a kind of low-cost and readily available industrial byproduct, ethylene tar (ET) was for the first time utilized for the preparation of heat-resistant condensed polynuclear aromatic resin (COPNAR). The basic properties of ET and the resulted COPNAR were characterized by FT-IR, 1H-NMR, TGA and elemental analysis. The test results showed that ET with high aromatic content (>50%) was suitable for the synthesis of COPNAR with superior heat resistance. The average molecular structure of ET was obtained by means of the improved Brown-Ladner method, and the reaction mechanism was considered as an acid-catalyzed positive ion-typed polymerization. Our findings have provided a new route to develop ET into technology-added heat-resistant resins. | Wu Mingbo Shi Yangyang Li Shibin Wang Yuwei Tan Minghui Wang Ding Zheng Jingtang Tsubaki Noritatsu | 2012 | China Petroleum Processing & Petrochemical Technology2012，14，4： | 7 |
| 8 | Exosomal LINC00355 derived from cancer-associated fibroblasts promotes bladder cancer cell resistance to cisplatin by regulating miR-34b-5p/ABCB1 axis显示文摘Cisplatin resistance is a major challenge for bladder cancer(BC).Evidence indicates that exosome derived from cancer-associated fibroblasts(CAF-Exo)can promote chemotherapy resistance in various human tumors by delivering bioactive molecules.We have previously demonstrated that CAF-derived exosomal LINC00355 promotes BC cell proliferation and invasion.However,the underlying mechanisms are still unclear.In this study,we aimed to investigate the role and mechanisms of CAF-derived exosomal LINC00355 in BC cell resistance to cisplatin.Exosomes were isolated from normal fibroblasts(NFs)and BC tumor-derived CAFs,namely,NF-Exo and CAF-Exo.CAFs were transfected with si-Ctrl or si-LINC00355 and then different exosomes were isolated,namely,CAF^(si-Ctrl)-Exo and CAF^(si-LINC00355)-Exo.The human BC cell lines(T24 and 5367)were incubated with NF-Exo,CAF-Exo,CAF^(si-Ctrl)-Exo,and CAF^(si-LINC00355)-Exo in the presence of cisplatin.MTT proliferation assay and flow cytometric analysis showed that CAF-Exo promoted BC cell resistance to cisplatin and upregulated ABCB1 expression in BC cells by transferring LINC00355 to BC cells.Luciferase activity assay confirmed the interaction between miR-34b-5p and LINC00355 or ABCB1.qRT-PCR and western blot analysis further showed that LINC00355 sponged miR-34b-5p to upregulate ABCB1 expression.However,the promoting effects of CAF-Exo on BC cell resistance to cisplatin were abolished by miR-34b-5p overexpression and ABCB1 silencing.In conclusion,exosomal LINC00355 derived from CAFs promotes BC cell resistance to cisplatin by regulating the miR-34b-5p/ABCB1 axis. | Guangyue Luo Yangyang Zhang Zhonghui Wu Ligang Zhang Chaozhao Liang Xianguo Chen | 2021 | Acta Biochimica et Biophysica Sinica2021，53，5： | 6 |
| 9 | Recent advances on deoximation: From stoichiometric reaction to catalytic reaction显示文摘Deoximation reaction is one of the most important transformations in organic synthesis and for fine chemical production.Since oximes are easily synthesized from carbonyl compounds and are stable compounds,this reaction can be used for protection-deprotection,purification,and characterization of carbonyl compounds in organic synthesis,especially for the synthesis of medicines as well as the natural products.Moreover,because many oximes can be synthesized from non-carbonyl starting materials,the deoximation reaction is also widely used in the production of many carbonyl-contained fine chemicals.Deoximation methods by using stoichiometric reagents are mature and can produce the related carbonyl products in very high yield with broad substrate application scopes.But for environment-protection consideration as well as the production cost controlling purpose in fine chemical industry,developing catalytic deoximation methods is the trend of the field and there are a series of references on this topic in recent years.This short review summarized recent advances on the development of deoximation methods from stoichiometric reaction to catalytic reaction and the mechanisms of some important transformations were discussed in detail for reader reference. | Yinghao Zheng Aiqiong Wu Yangyang Ke Hongen Cao Lei Yu | 2019 | Chinese Chemical Letters2019，30，5： | 5 |
| 10 | Coronary artery bypass grafting with concomitant resection for carcinoma of lung显示文摘A 69-year-old woman with angina had a lesion in the left lower lobe on chest film.Angiography revealed coronary artery disease in three vessels.Combined off pump coronary artery bypass grafting(CABG)and left lower lobectomy were performed through median sternotomy.This approach avoids complications due to staged operations and cardiopulmonary bypass(CPB).This report shows that simultaneous off pump CABG and pulmonary operations can be performed safely in patients with coronary artery disease(CAD)associated with lung cancer. | Yangyang Zhang,Yanhu Wu ,Biao Yuan,Xiang Liu,Sheng Zhao,Zhi Li,Yu Xia Department of Cardiothoracic Surgery,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,Jiangsu Province,China | 2010 | The Journal of Biomedical Research2010，24，1： | 4 |
| 11 | The use of supercytokines, immunocytokines, engager cytokines, and other synthetic cytokines in immunotherapy显示文摘Cytokines exert powerful immunomodulatory effects that are critical to physiology and pathology in humans.The application of natural cytokines in clinical studies has not been clearly established,and there are often problems associated with toxicity or lack of efficacy.The key reasons can be attributed to the pleiotropy of cytokine receptors and undesired activation of off-target cells.With a deeper understanding of the structural principles and functional signals of cytokine-receptor interactions,artificial modification of cytokine signaling through protein engineering and synthetic immunology has become an increasingly feasible and powerful approach.Engineered cytokines are designed to selectively target cells.Herein,the theoretical and experimental evidence of cytokine engineering is reviewed,and the“supercytokines”resulting from structural enhancement and the“immunocytokines”generated by antibody fusion are described.Finally,the“engager cytokines”formed by the crosslinking of cytokines and bispecific immune engagers and other synthetic cytokines formed by nonnatural analogs are also discussed. | Xiaohu Zheng Yaqi Wu Jiacheng Bi Yingying Huang Ying Cheng Yangyang Li Yuwei Wu Guoshuai Cao Zhigang Tian | 2022 | Cellular & Molecular Immunology2022，19，2： | 4 |
| 12 | Low carbon storage of woody debris in a karst forest in southwestern China显示文摘The properties of woody debris(WD) vary across different forests under various soil conditions.Owing to the relatively shallow and low amounts of soils on karst terrains, it is necessary to determine the WD carbon inventory of karst forests. In this study, we recorded WD with a basal diameter for standing snags and the largeend diameter for fallen logs of ≥ 1 cm. The carbon density of WD in a secondary karst mixed evergreen and deciduous broad-leaved forest that had been clear-cut 55 years ago in southwestern China were inventoried in a 2 ha plot. Woody debris carbon density calculated using specific gravity and carbon concentration was 4.07 Mg C ha^-1. Woody debris with diameters ≥ 10 cm(coarse WD) constituted 53.8% of total carbon storage whereas WD < 10 cm in diameters(fine WD) accounted for more pieces of WD(89.9%).Lithocarpus confinis contributed the most WD carbon(26.5%). Intermediate decayed WD was relatively more abundant, but WD with final decay contributed the least to the total pieces of WD(6.7%). The contribution of WD to carbon storage of karst forest was low compared to other forests worldwide. Significant positive correlations were found between WD carbon and biodiversity(R^2= 0.035,p < 0.01) and elevation(R^2= 0.047, p < 0.01) and negative correlations was found in outcrop coverage(R^2= 0.034, p <0.01). Further studies are needed to elucidate the ecological functions of WD to better understand their roles in maintaining biodiversity, enhancing productivity, and controlling vegetation degradation in karst forest ecosystems. | Yangyang Wu Libin Liu Chunzi Guo Zhonghua Zhang Gang Hu Jian Ni | 2019 | Acta Geochimica2019，38，4： | 3 |
| 13 | Differential equation dynamical system based assessment model in GNSS interoperability显示文摘With the development of Global Navigation Satellite System (GNSS),the idea of GNSS interoperability is born and has become the focus of study in the field of satellite navigation.The popularity for GNSS to augment the interoperability with the existing ones necessitates the study of the assessment algorithm of this idea.In this paper,an assessment algorithm for interoperability comprehensive benefits based on the differential equation dynamical system is discussed.There are two important aspects in GNSS that interoperability will affect:one is the performance advancement;the other one is the cost of adopting interoperability.While researching the complex relationship between the performance and cost,we found this relationship is similar as what between prey and predator in biomathematics,so the Lotka-Volterra model used to depict the prey-predator relationship is a felicitous tool.After building a differential dynamical model,we analyze the existence and stability of the positive equilibrium in the model.Then a Cost-Effective Function of GNSS is constructed based on the positive equilibrium,which is employed to assess the interoperability,qualitatively and quantitatively.Finally,the paper demonstrates the significance of the model and its application by citing a numerical example. | HAN Tao LU XiaoChun WANG Xue RAO YongNan ZOU DeCai YANG JianFei WU YangYang | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，6： | 3 |
| 14 | Robust exponential stability of uncertain discretetime impulsive switching systems with state delay显示文摘A new class of hybrid impulsive and switching models are introduced and their robust exponential stability and control synthesis are addressed.The proposed switched system is composed of stable subsystems and unstable subsys- tems,which not only involves state delay and norm-bounded time-varying parameter uncertainties,but also contains the impulsive switching effects between the subsystems.Based on the extension of the system dimension and the concept of average dwell time,a kind of practically useful switching rule is presented which guarantees the desired robust exponential stability.A switched state feedback controller is also given. | Guangdeng ZONG Yuqiang WU Baoyong ZHANG Yangyang KONG | 2007 | 控制理论与应用(英文版)2007，5，4： | 2 |
| 15 | In-situ microstructural investigations of the TRIP-to-TWIP evolution in Ti-Mo-Zr alloys as a function of Zr concentration显示文摘Aiming at overcoming the strength-ductility trade-off in structural Ti-alloys,a new family of TRIP/TWIP Ti-alloys was developed in the past decade(TWIP:twinning-induced plasticity;TRIP:transformationinduced plasticity).Herein,we study the tunable nature of deformation mechanisms with various TWIP and TRIP contributions by fine adjustment of the Zr content on ternary Ti-12 Mo-xZr(x=3,6,10)alloys.The microstructure and deformation mechanisms of the Ti-Mo-Zr alloys are explored by using in-situ electron backscatter diffraction(EBSD)and transmission electron microscopy(TEM).The results show that a transition of the dominant deformation mode occurred,going from TRIP to TWIP major mechanism with increasing Zr content.In the Ti-12 Mo-3 Zr alloy,the stress-induced martensitic transformation(SIM)is the major deformation mode which accommodates the plastic flow.Regarding the Ti-12 Mo-6 Zr alloy,the combined deformation twinning(DT)and SIM modes both contribute to the overall plasticity with enhanced strain-hardening rate and subsequent large uniform ductility.Further increase of the Zr content in Ti-12 Mo-10 Zr alloy leads to an improved yield stress involving single DT mode as a dominant deformation mechanism throughout the plastic regime.In the present work,a set of comprehensive in-situ and ex-situ microstructural investigations clarify the evolution of deformation microstructures during tensile loading and unloading processes. | Bingnan Qian Jinyong Zhang Yangyang Fu Fan Sun Yuan Wu Jun Cheng Philippe Vermaut Frederic Prima | 2021 | Journal of Materials Science & Technology2021，，6： | 2 |
| 16 | Sources of organic matter and paleo-environmental implications inferred from carbon isotope compositions of lacustrine sediments at Inexpressible Island, Ross Sea, Antarctica显示文摘The carbon isotopic composition of organic matter(δ^(13)C_(org)) was determined in two sediment cores(IIL1 and IIL9) recovered from Inexpressible Island, Ross Sea, Antarctica, and analyzed to identify the sources of that organic matter. The δ^(13)C_(org) values of sediments of IIL9 were found to vary between-14.6‰ and-11.6‰, with a mean of-13.4‰(n=48). These values were significantly higher than those of IIL1 sediments which varied between-23.2‰ and-20.4‰, with a mean of-21.8‰(n=55). The variation in δ^(13)C_(org) values in these two sediment cores indicate different sources of organic matter. The relatively high δ^(13)C_(org) values in IIL9 are in accordance with a source from algae, while the low δ^(13)C_(org) values in IIL1 evince significant influence from penguin guano with algae as the secondary source. Compared with the reference data from other high-latitude lake sediments and plants, the δ^(13)C_(org) values in IIL9 were extremely high, a result likely related to intense competition for CO_2 assimilation among algal species during the growing season in this relatively shallow pond. These results indicate that sedimentary δ^(13)C_(org) is a reliable proxy for paleo-primary productivity in ponds at Inexpressible Island. | WEI Yangyang JIN Jing NIE Yaguang CHEN Xin WU Libin FU Pingqing LIU Xiaodong Steven D.Emslie | 2016 | Advances in Polar Science2016，27，4： | 2 |
| 17 | Experimental and Numerical Analysis of High-Strength Concrete Beams Including Steel Fibers and Large-Particle Recycled Coarse Aggregates显示文摘In order to study the performances of high-strength concrete beams including steel fibers and large-particle recycled aggregates,four different beams have been designed,tested experimentally and simulated numerically.As varying parameters,the replacement rates of recycled coarse aggregates and CFRP(carbon fiber reinforced polymer)sheets have been considered.The failure mode of these beams,related load deflection curves,stirrup strain and shear capacity have been determined through monotonic loading tests.The simulations have been conducted using the ABAQUS finite element software.The results show that the shear failure mode of recycled concrete beams is similar to that of ordinary concrete beams.The shear carrying capacity of high-strength concrete beams including steel fibers and large-particle recycled coarse aggregates grows with an increase in the replacement rate of recycled coarse aggregates.Reinforcement with CFRP sheets can significantly improve the beam’s shear carrying capacity and overall resistance to deformation. | Chunyang Liu Yangyang Wu Yingqi Gao Zhenyun Tang | 2021 | Fluid Dynamics & Materials Processing2021，17，5： | 2 |
| 18 | Chromobox homolog 4 overexpression inhibits TNF-α-induced matrix catabolism and senescence by suppressing activation of NF-κB signaling pathway in nucleus pulposus cells显示文摘Intervertebral disc degeneration(IDD)is featured as enhanced catabolism of extracellular matrix(ECM)in the nucleus pulposus(NP),in which tumor necrosis factor-alpha(TNF-α)-related cell senescence is involved.Chromobox homolog protein 4(CBX4)exhibits anti-inflammatory effects and shows promising therapeutic potential.Thus,in the present study,we explore the role of CBX4 in IDD.Immunohistochemistry staining reveals that CBX4 expression is decreased in severe degenerative NP tissues compared to mild degenerative tissues,and real-time PCR and western blot analysis results show that CBX4 expression is downregulated under TNF-αstimulation in NP cells.siRNA and adenoviruses are used to knockdown or overexpress CBX4,respectively.The results demonstrate that CBX4 knockdown augments the catabolism of ECM in human NP cells,while CBX4 overexpression in rat NP cells restores the ECM degradation induced by TNF-α,as illustrated by immunofluorescence and western blot analysis.In addition,transcriptome sequencing results reveal the regulatory effect of CBX4 on the cell cycle,and further western blot analysis and senescence-associatedβ-galactosidase staining assay indicate that CBX4 overexpression alleviates cell senescence in the presence of TNF-α.Moreover,the phosphorylation of p65,which indicates the activation of NF-κB signaling,is measured by western blot analysis and immunofluorescence assay,and the results reveal that CBX4 overexpression reduces the TNF-α-induced increase in the p-p65/p65 ratio.In addition,the effect of CBX4 overexpression in NP cells is suppressed by NF-κB agonist.In summary,our results indicate that CBX4 overexpression can suppress TNF-α-induced matrix catabolism and cell senescence in the NP by inhibiting NF-κB activation.This study may provide new approaches for preventing and treating IDD. | Yangyang Zhang Shuangxing Li Junmin Hong Jiansen Yan Zhengqi Huang Jiajun Wu Zhihuai Deng Tianyu Qin Kang Xu Wei Ye | 2022 | Acta Biochimica et Biophysica Sinica2022，54，7： | 2 |
| 19 | High-metallic-phase-concentration MO1-xWxS2 nanosheets with expanded interlayers as efficient electrocatalysts显示文摘 | Qun He Yangyang Wan Hongliang Jiang Chuanqiang Wu Zhongti Sun Shuangming Chen Yu Zhou Haiping Chen Daobin Liu Yasir A. Haleem Binghui Ge Xiaojun Wu Li Song | 2018 | Nano Research2018，11，3： | 1 |
| 20 | Vehicle emissions of primary air pollutants from 2009 to 2019 and projection for the 14th Five-Year Plan period in Beijing, China显示文摘Over the past decade,the emission standards and fuel standards in Beijing have been upgraded twice,and the vehicle structure has been improved by accelerating the elimination of 2.95 million old vehicles.Through the formulation and implementation of these policies,the emissions of carbon monoxide(CO),volatile organic compounds(VOCs),nitrogen oxides(NO_(x)),and fine particulate matter(PM_(2.5))in 2019 were 147.9,25.3,43.4,and 0.91 kton in Beijing,respectively.The emission factor method was adopted to better understand the emissions characteristics of primary air pollutants from combustion engine vehicles and to improve pollution control.In combination with the air quality improvement goals and the status of social and economic development during the 14th Five-Year Plan period in Beijing,different vehicle pollution control scenarios were established,and emissions reductions were projected.The results show that the emissions of four air pollutants(CO,VOCs,NO_(x),and PM_(2.5))fromvehicles in Beijing decreased by an average of 68% in 2019,compared to their levels in 2009.The contribution of NOx emissions from diesel vehicles increased from 35% in 2009 to 56% in 2019,which indicated that clean and energy-saving diesel vehicle fleets should be further improved.Electric vehicle adoption could be an important measure to reduce pollutant emissions.With the further upgrading of vehicle structure and the adoption of electric vehicles,it is expected that the total emissions of the four vehicle pollutants can be reduced by 20%-41% by the end of the 14th Five-Year Plan period. | Tongran Wu Yangyang Cui Aiping Lian Ye Tian Renfei Li Xinyu Liu Jing Yan Yifeng Xue Huan Liu Bobo Wu | 2023 | Journal of Environmental Sciences2023，，2： | 1 |
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