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| 1 | Ginkgo biloba extract improved cognitive and neurological functions of acute ischaemic stroke:a randomised controlled trial显示文摘Purpose To evaluate the efficacy and safety of Ginkgo biloba extract(GBE)in acute ischaemic stroke and its impact on the recurrence of vascular events.Methods We conducted a multicentre,prospective,randomised,open label,blinded,controlled clinical trial enrollingpatients with an onset of acute stroke within 7 days from five hospitals in China Jiangsu Province.Participants were assigned to the GBE group(450 mg GBE with 100 mg aspirin daily)or the control group(100 mg aspirin daily)for 6 months.The primary outcome was the decline in the Montreal Cognitive Assessment score at 6 months.Secondary outcomes were other neuropsychological tests of cognitive and neurological function,the the incidence of adverse events and vascular events.results 348 patients were enrolled:179 in the GBE group and 169 in the control group.With 18 patients lost to follow-up,the dropout rate was 5.17%.Admission data between two groups were similar,but in the GBE group there was a marked slow down in the decline in the Montreal Cognitive Assessment scores(−2.77±0.21 vs−1.99±0.23,P=0.0116(30 days);−3.34±0.24 vs−2.48±0.26,P=0.0165(90 days);−4.00±0.26 vs−2.71±0.26,P=0.0004(180 days))compared with controls.The National Institutes of Health Stroke Scale scores at 12 and 30 days,the modified Rankin Scale scores for independent rate at 30,90 and 180 days,and the Barthel Index scores at 30,90 and 180 days in the GBE group were significantly improved compared with controls.Improvements were also observedin GBE groups for Mini-Metal State Examination scores of 30,90 and 180 days,Webster's digit symbol test scores at 30 days and Executive Dysfunction Index scores at 30 and 180 days.No significant differences were seen in the incidence of adverse events or vascular events.conclusions We conclude that GBE in combination with aspirin treatment alleviated cognitive and neurological deficits after acute ischaemic stroke without increasing the incidence of vascular events. | Shanshan Li Xinjiang Zhang Qi Fang Junshan Zhou Meijuan Zhang Hui Wang Yan Chen Biyun Xu Yanfeng Wu Lai Qian Yun Xu | 2017 | Stroke & Vascular Neurology2017，2，4： | 25 |
| 2 | Graphene: a promising 2D material for electrochemical energy storage显示文摘Graphene, with unique two-dimensional form and numerous appealing properties, promises to remarkably increase the energy density and power density of electrochemical energy storage devices(EESDs),ranging from the popular lithium ion batteries and supercapacitors to next-generation high-energy batteries. Here, we review the recent advances of the state-of-the-art graphene-based materials for EESDs,including lithium ion batteries, supercapacitors, micro-supercapacitors, high-energy lithium-air and lithium-sulfur batteries, and discuss the importance of the pore, doping, assembly, hybridization and functionalization of different nano-architectures in improving electrochemical performance. The major roles of graphene are highlighted as(1) a superior active material,(2) ultrathin 2D flexible support,and(3) an inactive yet electrically conductive additive. Furthermore, we address the enormous potential of graphene for constructing new-concept emerging graphene-enabled EESDs with multiple functionalities of lightweight, ultra-flexibility, thinness, and novel cell configurations. Finally, future perspectives and challenges of graphene-based EESDs are briefly discussed. | Yanfeng Dong Zhong-Shuai Wu Wencai Ren Hui-Ming Cheng Xinhe Bao | 2017 | Science Bulletin2017，62，10： | 22 |
| 3 | Age-related rhesus macaque models of COVID-19显示文摘Background:Since December 2019,an outbreak of the Corona Virus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus(SARS-CoV-2)in Wuhan,China,has become a public health emergency of international concern.The high fatality of aged cases caused by SARS-CoV-2 was a need to explore the possible age-related phenomena with non-human primate models.Methods:Three 3-5 years old and two 15 years old rhesus macaques were intratracheally infected with SARS-CoV-2,and then analyzed by clinical signs,viral replication,chest X-ray,histopathological changes and immune response.Results:Viral replication of nasopharyngeal swabs,anal swabs and lung in old monkeys was more active than that in young monkeys for 14 days after SARS-CoV-2 challenge.Monkeys developed typical interstitial pneumonia characterized by thickened alveolar septum accompanied with inflammation and edema,notably,old monkeys exhibited diffuse severe interstitial pneumonia.Viral antigens were detected mainly in alveolar epithelial cells and macrophages.Conclusion:SARS-CoV-2 caused more severe interstitial pneumonia in old monkeys than that in young monkeys.Rhesus macaque models infected with SARS-CoV-2 provided insight into the pathogenic mechanism and facilitated the development of vaccines and therapeutics against SARS-CoV-2 infection. | Pin Yu Feifei Qi Yanfeng Xu Fengdi Li Peipei Liu Jiayi Liu Linlin Bao Wei Deng Hong Gao Zhiguang Xiang Chong Xiao Qi Lv Shuran Gong Jiangning Liu Zhiqi Song Yajin Qu Jing Xue Qiang Wei Mingya Liu Guanpeng Wang Shunyi Wang Haisheng Yu Xing Liu Baoying Huang Wenling Wang Li Zhao Huijuan Wang Fei Ye Weimin Zhou Wei Zhen Jun Han Guizhen Wu Qi Jin Jianwei Wang Wenjie Tan Chuan Qin | 2020 | Animal Models and Experimental Medicine2020，3，1： | 22 |
| 4 | Low-dose decitabine enhances the effect of PD-1 blockade in colorectal cancer with microsatellite stability by re-modulating the tumor microenvironment显示文摘PD-1 blockade has demonstrated impressive clinical outcomes in colorectal cancers that have high microsatellite instability.However,the therapeutic efficacy for patients with tumors with low microsatellite instability or stable microsatellites needs further improvement.Here,we have demonstrated that low-dose decitabine could increase the expression of immune-related genes such as major histocompatibility complex genes and cytokine-related genes as well as the number of lymphocytes at the tumor site in CT26 colorectal cancer-bearing mice.A more significant inhibition of tumor growth and a prolongation of survival were observed in the CT26 mouse model after treatment with a combination of PD-1 blockade and decitabine than in mice treated with decitabine or PD-1 blockade alone.The anti-tumor effect of the PD-1 blockade was enhanced by low-dose decitabine.The results of RNA sequencing and whole-genome bisulfite sequencing of decitabine-treated CT26 cells and tumor samples with microsatellite stability from the patient tumor-derived xenograft model have shown that many immune-related genes,including antigen-processing and antigen-presenting genes,were upregulated,whereas the promoter demethylation was downregulated after decitabine exposure.Therefore,decitabine-based tumor microenvironment re-modulation could improve the effect of the PD-1 blockade.The application of decitabine in PD-1 blockade-based immunotherapy may elicit more potent immune responses,which can provide clinical benefits to the colorectal cancer patients with low microsatellite instability or stable microsatellites. | Ganjun Yu Yanfeng Wu Wenying Wang Jia Xu Xiaoping Lv Xuetao Cao Tao Wan | 2019 | Cellular & Molecular Immunology2019，16，4： | 14 |
| 5 | TLR4 is essential for dendritic cell activation and anti-tumor T-cell response enhancement by DAMPs released from chemically stressed cancer cells显示文摘免疫疗法和化疗的联合为癌症的某些类型的治疗被认为是一条有希望的途径。然而,内在的机制需要充分被调查为癌症 chemoimmunotherapy 指导更有效的协议的设计。联系危险的分子的模式(阻尼) 能激活有免疫力的房间,是众所周知的,包括树枝状的房间(DC ) ,经由像使用费的受体(TLR ) ;然而,在有免疫力的反应的激活免除化学对待药的肿瘤房间的阻尼的角色需要进一步被阐明。这里,我们发现那 colorectal 与 oxaliplatin (OXA ) 对待的癌症(CRC ) 房间或 5 氟尿嘧啶(5-Fu ) 释放了高活动性的组盒子 1 的高水平(HMGB1 ) 和热吃惊蛋白质 70 (HSP70 ) 。在 OXA/5-Fu 治疗以后,也展出的 CRC 病人的 sera 增加了 HMGB1 和 HSP70 的层次,哪个是著名阻尼。与 OXA/5-Fu 对待的垂死的 CRC 房间的上层清液支持了老鼠和人的 DC 成熟,与 HLA 医生, CD80 和 CD86 表示和 IL-1β 的改进的 upregulation;, TNF-α, MIP-1α, MIP-1β, RANTES 和 IP-10 生产。由 DC 组成的疫苗与导致的化学上强调的 CRC 房间的上层清液搏动了更重要的 IFN-γ在 vitro 并且在 vivo 生产 Th1 反应。然而,化学上强调的 CRC 房间的上层清液没能在 TLR4 缺乏的 DC 导致 phenotypic 成熟和 cytokine 生产,显示在导致阻尼的 DC 成熟和激活的 TLR4 的一个必要角色。而且,有化学上强调的 CRC 房间的上层清液的 pulsing 高效地没导致 IFN-γ在 TLR4 缺乏的 DC 生产 Th1 反应。一起,这些结果证明免除化学上强调的癌症房间的阻尼能经由 TLR4 激活 DC 并且提高反肿瘤 T 房间有免疫力的回答的正式就职,描出一条临床上相关的免疫助手小径由阻尼被触发。 | Hongliang Fang Bing Ang Xinyun Xu Xiaohui Huang Yanfeng Wu Yanping Sun Wenying Wang Nan Li Xuetao Cao Tao Wan | 2014 | Cellular & Molecular Immunology2014，11，2： | 11 |
| 6 | Spatio-temporal patterns of drought in North Xinjiang, China, 1961–2012 based on meteorological drought index显示文摘Drought, which is one of the most frequently occurring severe hazards with long time scales and covering wide geographical areas, is a natural phenomenon resulting in significant economic losses in agriculture and industry. Drought is caused by an imbalance between the inputs of and the demand for water which is insufficient to meet the demands of human activities and the eco-environment. As a major arid and semi-arid area and an important agricultural region in Northwest China, North Xinjiang(NX) shows great vulnerability to drought. In this paper, the characteristics of inter-annual and seasonal drought were analyzed in terms of drought occurrence and drought coverage, by using the composite index of meteorological drought and the data of daily precipitation, air temperature, wind speed, relative humidity and sunshine duration from 38 meteorological stations during the period 1961–2012. Trend analysis, wavelet analysis and empirical orthogonal function were also applied to investigate change trend, period and regional characteristics, respectively. In NX, annual and seasonal drought occurrence and drought coverage all showed a decreasing trend that was most significant in winter(with rates of –0.26 month/10 a and –15.46%, respectively), and drought occurrence in spring and summer were more frequent than that in autumn and winter. Spatially, drought was severe in eastern regions but mild in western regions of NX. Annual and seasonal drought occurrence at 38 meteorological stations displayed decreasing trends and were most significant in 'Shihezi-Urumqi-Changji', which can help to alleviate severe drought hazards for local agricultural production and improve human livelihood. NX can be approximately classified into three sub-regions(severe drought region, moderate drought region and mild drought region), which were calculated from annual drought frequencies. The cross wavelet transform suggested that SOI(Southern Oscillation Index), AOI(Arctic Oscillation Index), AAOI(Antarctic Oscillation Index), PAOI(Pacific/North American Oscillation Index) and NAOI(North Atlantic Oscillation Index) have significant correlation with the variation of drought occurrence in NX. To prevent and mitigate the occurrence of drought disasters in NX, agricultural and government managers should pay more attention to those drought events that occur in spring and summer. | WU Yanfeng Batur BAKE ZHANG Jusong Hamid RASULOV | 2015 | Journal of Arid Land2015，7，4： | 8 |
| 7 | Efficient and Large-Scale Synthesis of Few-Layered Graphene Using an Arc-Discharge Method and Conductivity Studies of the Resulting Films显示文摘An arc-discharge method using a buffer gas containing carbon dioxide has been developed for the efficient and large-scale synthesis of few-layered graphene.The resulting samples of few-layered graphene,well-dispersed in organic solvents such as N,N-dimethylformamide(DMF)and 1,2-dichlorobenzene(o-DCB),were examined by transmission electron microscopy(TEM),X-ray diffraction(XRD),Raman spectroscopy,atomic force microscopy(AFM),and thermal gravimetric analysis(TGA).The electrical conductivity and transparency of flexible films prepared using a direct solution process have also been studied. | Yingpeng Wu Bin Wang Yanfeng Ma Yi Huang Na Li Fan Zhang Yongsheng Chen | 2010 | Nano Research2010，3，9： | 8 |
| 8 | The role of climate factors in geographic variation in body mass and wing length in a passerine bird显示文摘Background: Geographic variation in body size is assumed to reflect adaptation to local environmental conditions. Although Bergmann's rule is usually sufficient to explain such variation in homeotherms, some exceptions have been documented. The relationship between altitude, latitude and body size, has been well documented for some vertebrate taxa during the past decades. However, relatively little information is available on the effects of climate variables on body size in birds.Methods: We collected the data of 267 adult Eurasian Tree Sparrow(Passer montanus) specimens sampled at 48 localities in China's mainland, and further investigated the relationships between two response variables, body mass and wing length, as well as a suit of explanatory variables, i.e. altitude, latitude, mean annual temperature(MAT), annual precipitation(PRC), annual sunshine hours(SUN), average annual wind speed(WS), air pressure(AP) and relative humidity(RH).Results: Our study showed that(1) although the sexes did not differ significantly in body mass, males had longer wings than females;(2) body mass and wing length were positively correlated with altitude but not with latitude;(3) body mass and wing length were negatively correlated with AP and RH, but not significantly correlated with WS. Body mass was positively correlated with SUN and inversely correlated with MAT. Wing length was not correlated with MAT in either sex, but was positively correlated with SUN and negatively correlated with PRC in male sparrows;(4) variation in body mass could be best explained by AP and SUN, whereas variation in wing length could be explained by RH and AP in both sexes. In addition, variation in male sparrows can be explained by SUN, WS and PRC but not in females.Conclusions: Two different proxies of body size, body mass and wing length, correlated with same geographic factors and different climate factors. These differences may reflect selection for heat conservation in the case of body mass, and for efficient flight in the case of wing length. | Yanfeng Sun Mo Li Gang Song Fumin Lei Dongming Li Yuefeng Wu | 2017 | Chinese Birds2017，，1： | 7 |
| 9 | Blunted nitric oxide regulation in Tibetans under high-altitude hypoxia显示文摘Nitric oxide(NO) is an important molecule for vasomotor tone, and elevated NO signaling was previously hypothesized as a unique and adaptive physiological change in highland Tibetans. However, there has been lack of NO data from Tibetans living at low altitude and lowlander immigrants living at high altitude, which is crucial to test this hypothesis. Here, through cross-altitude(1990–5018 m) and cross-population(Tibetans and Han Chinese) analyses of serum NO metabolites(NOx) of 2086 individuals, we demonstrate that although Tibetans have a higher serum NOx level compared to lowlanders, Han Chinese immigrants living at high altitude show an even higher level than Tibetans. Consequently, our data contradict the previous proposal of increased NO signaling as the unique adaptive strategy in Tibetans.Instead, Tibetans have a relatively lower circulating NOx level at high altitude. This observation is further supported by data from the hypoxic experiments using human umbilical vein endothelial cells and gene knockout mice. No difference is detected between Tibetans and Han Chinese for endothelial nitric oxide synthase(e NOS), the key enzyme for circulating NO synthesis, suggesting that e NOS itself is unlikely to be the cause. We show that other NO synthesis-related genes(e.g. GCH1) carry Tibetan-enriched mutations significantly associated with the level of circulating NOx in Tibetans. Furthermore, gene network analysis revealed that the downregulation and upregulation of NOx is possibly achieved through distinct pathways.Collectively, our findings provide novel insights into the physiological and genetic mechanisms of the evolutionary adaptation of Tibetans to high-altitude hypoxia. | Yaoxi He Xuebin Qi Ouzhuluobu Shiming Liu Jun Li Hui Zhang Baimakangzhuo Caijuan Bai Wangshan Zheng Yongbo Guo Duojizhuoma Baimayangji Dejiquzong Bianba Gonggalanzi Yongyue Pan Qula Kangmin Cirenyangji Wei Guo Yangla Yi Peng Xiaoming Zhang Kun Xiang Zhaohui Yang Liangbang Wang Gengdeng Yanfeng Zhang Tianyi Wu Bing Su Chaoying Cui | 2018 | National Science Review2018，5，4： | 7 |
| 10 | The spatial pattern of farming and factors influencing it during the Peiligang culture period in the middle Yellow River valley, China显示文摘The Peiligang culture(ca.7000–5000 cal BC)is the earliest Neolithic culture in the middle Yellow River valley and represents the emergence of cereal farming in the region[1].It has long been considered representative of millet farming[2],which requires a dry field,a certain amount of rainfall,and minimal crop management,but new records reveal that mixed farming of millet and rice(Oryza sp.)took place during this period[3].Therefore,it is possible | Can Wang Houyuan Lu Wanfa Gu Naiqin Wu Jianping Zhang Xinxin Zuo Fengjiang Li Daojing Wang Yajie Dong Songzhi Wang Yanfeng Liu Yingjian Bao Yayi Hu | 2017 | Science Bulletin2017，62，23： | 6 |
| 11 | IL-17A-dependent gut microbiota is essential for regulating diet-induced disorders in mice显示文摘The gut microbiota plays a key role in obesity and related metabolic disorders, and multiple factors including diet, host genotype, and age regulate it. Many studies have examined the contribution of extrinsic factors to the regulation of the gut microbiota, but the importance of the host genetic constitution cannot be ignored. Interleukin 17A(IL-17A), a pro-inflammatory cytokine, is important in the defense against infection and diseases. Here, we investigated the association among IL-17, a high-fat diet(HFD), and the gut microbiota. Mice deficient in IL-17A were resistant to diet-induced obesity and related diseases. Compared with the Ⅱ-17^(-/-)mice, wild-type(WT) mice challenged with HFD showed obvious weight fluctuations, such as those seen in type 2 diabetes, and hematological changes similar to those associated with metabolic syndrome. However, housing WT mice and Ⅱ-17a^(-/-)mice together significantly alleviated these symptoms in the WT mice. A metagenomic analysis of the mouse feces indicated that the microbial community compositions of these two groups differed before HFD feeding. The HFD mediated shifts in the gut microbial compositions, which were associated with the mouse phenotypes.We also identified potentially beneficial and harmful species present during this period, and drew networks of the most abundant species. A functional analysis indicated pathway changes in the WT and Ⅱ-17a^(-/-)mice when fed the HFD. Collectively, these data underscore the importance of the host factor IL-17 A in shaping and regulating the gut microbiota, which conversely, influences the host health. | Yujing Bi Chunxiao Li Lin Liu Jiyuan Zhou Zhengchao Li Huimin Deng Chunyan Wu Yanping Han Yajun Song Yafang Tan Xiaoyi Wang Zongmin Du Yujun Cui Yanfeng Yan Fachao Zhi Guangwei Liu Nan Qin Heping Zhang Ruifu Yang | 2017 | Science Bulletin2017，62，15： | 5 |
| 12 | Effects of capture and captivity on plasma corticosterone and metabolite levels in breeding Eurasian Tree Sparrows显示文摘Background: Bringing free-living animals into captivity subjects them to the stress of both capture and captivity, leading to the alteration of normal physiological processes and behaviors through activation of the hypothalamic– pituitary–adrenal axis. In free-living birds, although elevated plasma corticosterone (CORT) is an important adaptation regulating physiological and behavioral responses during the process of capture and captivity stress, little information is currently available on the effects of such stress on plasma metabolite levels. Methods: We examined the effects of immediate capture and 24-h captivity on body mass, body condition, plasma CORT, and metabolite levels including glucose (Glu), triglyceride (TG), total cholesterol (TC), uric acid (UA), in breeding Eurasian Tree Sparrows (Passer montanus). Results: CORT and Glu levels were increased significantly by the stress of capture, whereas TC and UA levels decreased. Body mass, body condition declined notably after 24 h in captivity, but CORT, Glu, and UA levels increased. Furthermore, male sparrows had lower TG levels after both capture and captivity than those of females. The relationships between plasma CORT and metabolite levels varied between sexes. Conclusions: Our results revealed that the metabolic status of Eurasian Tree Sparrows could be dramatically altered by capture and captivity. Monitoring the dynamic effects of both capture and captivity on plasma CORT, metabolite levels in a free-living bird contributes to a better understanding of the stress-induced pathways involved in sexdependent energy mobilization. | Mo Li Weiwei Zhu Yang Wang Yanfeng Sun Juyong Li Xuelu Liu Yuefeng Wu Xuebin Gao Dongming Li | 2019 | Avian Research2019，10，2： | 5 |
| 13 | Micelle or polymersome formation by PCL-PEG-PCL copolymers as drug delivery systems显示文摘Polye-caprolactone)-b-poly(ethylene glycol)-b-poly(e-caprolactone)(PCL-b-PEG-b-PCL,PCEC) triblock copolymers have been widely investigated in last several decades.Here,by altering the weight ratio of monomers in ring-opening polymerization,a series of PCEC triblock copolymers with varying hydrophobicity were synthesized,which were characterized by FTIR,1 H NMR,GPC and DSC.When PCEC copolymers with different weight ratios of PCL/PEG were dispersed in different aqueous solutions,they could self-assemble and form two distinctive nanoparticular structures:micelles or polymersomes.We then chose paclitaxel(PTX) as the model drug and encapsulate PTX into PCEC polymeric micelles and polymersomes.The physicochemical characterizations of the nanoparticles such as morphology,the size and distribution,zeta potential,drug loading content,and encapsulation efficiency were also performed.Our results showed that polymeric micelles or polymersomes from PCEC both displayed narrow size distributions and could achieve high drug loading efficiencies.In vitro cellular uptake results suggested that Nile Red loaded polymeric micelles or polymersomes displayed more internalization after 24 h incubation than those after 4 h incubation.These findings suggest that polymeric micelles and polymersomes based on PCL-b-PEG-b-PCL copolymers have great potential to effectively delivery hydrophobic drugs. | Chunyan Hu Zhuo Chen Shengjie Wu Yanfeng Han Hai Wang Hongfan Sun Deling Kong Xigang Leng Chun Wang Linhua Zhang Dunwan Zhu | 2017 | Chinese Chemical Letters2017，28，9： | 4 |
| 14 | 2D hierarchical yolk-shell heterostructures as advanced host-interlayer integrated electrode for enhanced Li-S batteries显示文摘Lithium sulfur(Li-S)batteries hold great promising for high-energy-density batteries,but appear rapid capacity fading due to the lack of overall and elaborated design of both sulfur host and interlayer.Herein,we developed a novel two-dimensional(2D)hierarchical yolk-shell heterostructure,constructed by a graphene yolk,2D void and outer shell of vertically aligned carbon-mediated MoS2 nanosheets(G@void@MoS2/C),as advanced host-interlayer integrated electrode for Li-S batteries.Notably,the 2D void,with a typical thickness of^80 nm,provided suitable space for loading and confining nano sulfur,and vertically aligned ultrathin MoS2 nanosheets guaranteed enriched catalytically active sites to effectively promote the transition of soluble polysulfides.The conductive graphene yolk and carbon mediated shell sufficiently accelerated electron transport.Therefore,the integrated electrode of G@void@MoS2/C not only exceptionally confined the sulfur/polysulfides in 2D yolk-shell heterostructures,but also achieved catalytic transition of the residual polysulfides dissolved in electrolyte to solid Li2S2/Li2S,both of which synergistically achieved an extremely low capacity fading rate of 0.05%per cycle over 1000 times at 2C,outperforming most reported Mo based cathodes and interlayers for Li-S batteries.2D hierarchical yolkshell heterostructures developed here may shed new insight on elaborated design of integrated electrodes for Li-S batteries. | Yanfeng Dong Pengfei Lu Haodong Shi Jieqiong Qin Jian Chen Wencai Ren Hui-Ming Cheng Zhong-Shuai Wu | 2019 | Journal of Energy Chemistry2019，28，9： | 4 |
| 15 | Percutaneous penetration enhancement effect of essential oil of mint (Mentha haplocalyx Briq.) on Chinese herbal components with different lipophilicity显示文摘Objective:To investigate the percutaneous penetration effect of essential oil of mint from Mentha haplocalyx Briq.on the complex active components in Chinese herbal external preparations,and assess its toxicity on the skin cells.Methods:The cytotoxicity of mint oil on HaCaT keratinocytes and CCC-ESF-1 fibroblasts was measured using an MTT assay.Five model drugs with a wide range of lipophilicity,namely osthole,tetramethylpyrazine,ferulic acid,puerarin,and geniposide,were tested using in vitro permeation studies to investigate the percutaneous penetration enhancement effect of mint oil.Secondary structure alterations of skin stratum corneum(SC)were measured using Fourier transform infrared spectroscopy(FTIR).Saturation solubilities and SC/vehicle partition coefficients of the five model drugs with and without mint oil were also determined to understand the potential mechanisms of the essential oil.Results:Half maximal inhibitory concentration(IC50)values of mint oil were significantly higher in HaCaT and CCC-ESF-1 cell lines than values in the well-established and standard penetration enhancer Azone.Conclusions:Mint oil at proper concentration could effectively facilitate percutaneous penetration of both lipophilic and hydrophilic drugs,and exhibit higher efficiency for moderate hydrophilic drugs.Mechanisms of penetration enhancement by mint oil could be explained with saturation solubility,SC/vehicle partition coefficient and the secondary structure change of SC. | Jingyan Wang Yi Lan Hui Li Yewen Zhang Qing Zhang Yanfeng Cao Qing Wu | 2014 | Journal of Traditional Chinese Medical Sciences2014，1，2： | 4 |
| 16 | A modified HLA-A^(*)0201-restricted CTL epitope from human oncoprotein (hPEBP4) induces more efficient antitumor responses显示文摘We previously identified human phosphatidylethanolamine-binding protein 4(hPEBP4)as an antiapoptotic protein with increased expression levels in breast,ovarian and prostate cancer cells,but low expression levels in normal tissues,which makes hPEBP4 an attractive target for immunotherapy.Here,we developed hPEBP4-derived immunogenic peptides for inducing antigen-specific cytotoxic T lymphocytes(CTLs)targeting breast cancer.A panel of hPEBP4-derived peptides predicted by peptide-MHC-binding algorithms was evaluated to characterize their HLAA2.1 affinity and immunogenicity.We identified a novel immunogenic peptide,P40–48(TLFCQGLEV),that was capable of eliciting specific CTL responses in HLA-A2.1/K^(b) transgenic mice,as well as in peripheral blood lymphocytes from breast cancer patients.Furthermore,amino-acid substitutions in the P40–48 sequence improved its immunogenicity against hPEBP4,a self-antigen,thus circumventing tolerance.We designed peptide analogs by preferred auxiliary HLA-A^(*)0201 anchor residue replacement,which induced CTLs that were crossreactive to the native peptide.Several analogs were able to stably bind to HLA-A^(*)0201 and elicit specific CTL responses better than the native sequence.Importantly,adoptive transfer of CTLs induced by vaccination with two analogs more effectively inhibited tumor growth than the native peptide.These data indicate that peptide analogs with high immunogenicity represent promising candidates for peptide-mediated therapeutic cancer vaccines. | Weihong Sun Junyi Shi Jian Wu Junchu Zhang Huabiao Chen Yuanyuan Li Shuxun Liu Yanfeng Wu Zhigang Tian Xuetao Cao Nan Li | 2018 | Cellular & Molecular Immunology2018，15，8： | 4 |
| 17 | Efficient induction of a Her2-specific anti-tumor response by dendritic cells pulsed with a Hsp70L1–Her2_(341–456) fusion protein显示文摘Heat shock proteins(HSPs)have been shown to interact with antigen-presenting cells(APCs),especially dendritic cells(DCs).HSPs act as potent adjuvants,inducing a Th1 response,as well as antigen-specific CD8^(+) cytotoxic T lymphocytes(CTL)via cross-presentation.Our previous work has demonstrated that Hsp70-like protein 1(Hsp70L1),a new member of the Hsp70 subfamily,can act as a powerful Th1 adjuvant in a DC-based vaccine.Here we report the efficient induction of tumor antigen-specific T cell immune response by DCs pulsed with recombinant fusion protein of Hsp70L1 and Her2_(341–456),the latter of which is a fragment of Her2/neu(Her2)containing E75(a HLA-A2 restricted CTL epitope).The fusion protein Hsp70L1–Her2_(341–456) promotes the maturation of DCs and activates them to produce cytokines,such as IL-12 and TNF-a,and chemokines,such as MIP-1a,MIP-1b and RANTES.Taken together,these results indicate that the adjuvant activity of Hsp70L1 is maintained in the fusion protein.Her2-specific HLA-A2.1-restricted CD8^(+) CTLs can be generated efficiently either from the Peripheral blood lymphocytes(PBL)of healthy donors or from the splenocytes of immunized HLA-A2.1/K^(b) transgenic mice by in vitro stimulation or immunization with DCs pulsed with the Hsp70L1–Her2_(341–456) fusion protein.This results in more potent target cell killing in an antigen-specific and HLA-A2.1-restricted manner.Adoptive transfer of splenocytes from transgenic mice immunized with Hsp70L1–Her2_(341–456)-pulsed DCs can markedly inhibit tumor growth and prolong the survival of nude mice with Her2^(+)/HLA-A2.1^(+) human carcinomas.These results suggest that Hsp70L1–Her2_(341–456)-pulsed DCs could be a new therapeutic vaccine for patients with Her2^(+) cancer. | Qiang Fu Yanfeng Wu Fang Yan Ning Wang Wenying Wang Xuetao Cao Yajie Wang Tao Wan | 2011 | Cellular & Molecular Immunology2011，8，5： | 3 |
| 18 | Nucleate Pool Boiling Heat Transfer of Pure Refrigerants and Binary Mixtures显示文摘Heat transfer coefficients in nucleate boiling on a smooth flat surface were measured for pure fluids of R-134a, propane, isobutane and their binary mixtures at different pressure from 0.1 to 0.6 MPa. Series of experiments with different heat flux and mixture concentrations were carried out. The influences of pressure and heat flux on the heat transfer coefficient for different pure fluids were studied. Isobutane and propane were used to make up binary mixtures. Compared to the pure components, binary mixtures show lower heat transfer coefficients. This reduction was more pronounced as the heat flux increasing. Several heat transfer correlations are obtained for different pure refrigerants and their binary mixtures. | Zhaofu SUN Maoqiong GONG Yanfeng QI Zhijian LI Jianfeng WU | 2004 | Journal of Thermal Science2004，13，3： | 3 |
| 19 | Pyridinic nitrogen enriched porous carbon derived from bimetal organic frameworks for high capacity zinc ion hybrid capacitors with remarkable rate capability显示文摘Aqueous zinc ion hybrid capacitors(ZIHCs)hold great potential for large-scale energy storage applications owing to their high safety and low cost,but suffer from low capacity and energy density.Herein,pyridinic nitrogen enriched porous carbon(nPC)was successfully synthesized via the growth,subsequent annealing and acid etching of bimetal organic frameworks for high capacity and safe ZIHCs with exceptional rate capability.Benefiting from the mesopores for easy ion diffusion,high electrical conductivity enabled by in-situ grown carbon nanotubes matrix and residual metal Co nanoparticles for fast electron transfer,sufficient micropores and high N content(8.9 at%)with dominated pyridinic N(54%)for enhanced zinc ion storage,the resulting nPC cathodes for ZIHCs achieved high capacities of 302 and137 m Ah g^(-1) at 1 and 18 A g^(-1),outperforming most reported carbon based cathodes.Theoretical results further disclosed that pyridinic N possessed larger binding energy of-4.99 eV to chemically coordinate with Zn2+than other N species.Moreover,quasi-solid-state ZIHCs with gelatin based gel electrolytes exhibited high energy density of 157.6 Wh kg^(-1) at 0.69 kW kg^(-1),high safety and mechanical flexibility to withstand mechanical deformation and drilling.This strategy of developing pyridinic nitrogen enriched porous carbon will pave a new avenue to construct safe ZIHCs with high energy densities. | Yao Li Pengfei Lu Ping Shang Lisha Wu Xiao Wang Yanfeng Dong Ronghuan He Zhong-Shuai Wu | 2021 | Journal of Energy Chemistry2021，30，5： | 3 |
| 20 | Morphology Effect of Metal-organic Framework HKUST-1 as a Catalyst on Benzene Oxidation显示文摘 | LIU Yanfeng LIU Boliang ZHOU Qingfeng ZHANG Tianyong WU Wubin | 2017 | Chemical Research in Chinese Universities2017，33，6： | 3 |
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