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| 1 | Functional tuning and expanding of myoglobin by rational protein design显示文摘Rational protein design is a powerful strategy,not only for revealing the structure and function relationship of natural metalloproteins,but also for creating artificial metalloproteins with improved properties and functions.Myoglobin(Mb),a small heme protein created by nature with diverse functions,has been shown to be an ideal scaffold for rational protein design.The progress reviewed herein includes fine-tuning its native functions of O2binding and transport,peroxidase activity and nitrite reductase(NIR)activity,and rational expanding its functionalities to peroxygenase,heme-copper oxidase(HCO),nitric oxide reductase(NOR),as well as hydroxylamine reductase.These studies have enhanced our understanding of how metalloproteins work in nature,and provided insights for rational design of functional metalloproteins for practical applications in the future. | LIN YingWu WANG JiangYun LU Yi | 2014 | Science China Chemistry2014，57，3： | 4 |
| 2 | Effectiveness and safety of endoscopic submucosal dissection for intraepithelial neoplasia of the esophagogastric junction显示文摘 | Wen Jing Linghu Enqiang Yang Yunsheng Liu Qingsen Yang Jing Wang Shufang Wang Xiangdong Du Hong Meng Jiangyun Wang Hongbin Lu Zhongsheng | 2014 | Chinese Medical Journal2014，，3： | 3 |
| 3 | NAD+ and its precursors in human longevity显示文摘 | Jimin Lin Yanchao Pan Jiangyun Wang | 2015 | Frontiers of Electrical and Electronic Engineering in China2015，10，4： | 3 |
| 4 | Crystal structure of E. synthetase and ligand revealed key residues coil arglnyl-tRNA binding studies in arginine recognition显示文摘 | Kelei Bi Yueting Zheng Feng Gao Jianshu Dong Jiangyun Wang Yi Wang Weimin Gong | 2014 | Protein & Cell2014，5，2： | 1 |
| 5 | Discrete algorithm for image scaling显示文摘 | Li jiangyun Yang shunnian Wang guozhao | 2003 | Journal of computeraided design & computer graphics2003，15，3： | 1 |
| 6 | Inside Cover: Genetic Incorporation of a Metal‐Chelating Amino Acid as a Probe for Protein Electron Transfer (Angew. Chem. Int. Ed. 41/2012)显示文摘 | Xiaohong Liu Jiasong Li Jianshu Dong Cheng Hu Weimin Gong Jiangyun Wang | 2012 | Angew Chem Int Ed2012，，41： | 1 |
| 7 | Effect of swirl on hydrodynamics and separation performance of a spray granulation tower with array nozzles显示文摘 | Meili Liu Yu Mao Jiangyun Wang Juan Wang Xuewen Sun Chunming Xu | 2011 | Powder Technology2011，，： | 1 |
| 8 | Ultrafast Photoinduced Electron Transfer in a Photosensitizer Protein显示文摘Photosystem Ⅱ(PSⅡ)is a large membrane protein(∼700 kDa)complex,harboring P680+,the strongest oxidant known in biological systems,which is responsible for driving tyrosine oxidation and ultimately O_(2) generation.While the enhancement and expansion of PSⅡ functions through genetic engineering would be beneficial for driving challenging chemical reactions,this has proven difficult due to its enormous complexity.Here,we report a genetically encoded,27 kDa photosensitizer protein(PSP3)that recapitulates the initial photoinduced key properties of PSⅡ.Through the genetic incorporation of benzophenone-alanine(BpA)into a fluorescent protein coupled with femtosecond transient absorption measurement,we show that photoinduced electron transfer from residue Tyr203 to the PSP3 chromophore occurs very rapidly(∼1 ps),which is comparable with that of the first electron transfer step in PSII.Since PSP3 can be overexpressed in high yield in Escherichia coli and genetically engineered easily,it might facilitate challenging oxidation and reduction reactions in vitro and in vivo. | Dandan Zheng Min Tao Longjiang Yu Xiaohong Liu Andong Xia Jiangyun Wang | 2022 | CCS Chemistry2022，4，4： | 1 |
| 9 | Biosynthetic approach to modeling and understanding metalloproteins using unnatural amino acids显示文摘Metalloproteins have inspired chemists for many years to synthesize artificial catalysts that mimic native enzymes.As a complementary approach to studying native enzymes or making synthetic models,biosynthetic approach using small and stable proteins to model native enzymes has offered advantages of incorporating non-covalent secondary sphere interactions under physiological conditions.However,most biosynthetic models are restricted to natural amino acids.To overcome this limitation,incorporating unnatural amino acids into the biosynthetic models has shown promises.In this review,we summarize first synthetic,semisynthetic and biological methods of incorporates unnatural amino acids(UAAs)into proteins,followed by progress made in incorporating UAAs into both native metalloproteins and their biosynthetic models to fine-tune functional properties beyond native enzymes or their variants containing natural amino acids,such as reduction potentials of azurin,O_2 reduction rates and percentages of product formation of HCO models in Mb,the rate of radical transport in ribonucleotide reductase(RNR)and the proton and electron transfer pathways in photosystemⅡ(PSⅡ).We also discuss how this endeavour has allowed systematic investigations of precise roles of conserved residues in metalloproteins,such as Metl21 in azurin,Tyr244 that is cross-linked to one of the three His ligands to CuB in HCO,Tyr122,356,730 and 731 in RNR and TyrZ in PSⅡ.These examples have demonstrated that incorporating UAAs has provided a new dimension in our efforts to mimic native enzymes and in providing deeper insights into structural features responsible high enzymatic activity and reaction mechanisms,making it possible to design highly efficient artificial catalysts with similar or even higher activity than native enzymes. | Yang Yu Chang Cui Jiangyun Wang Yi Lu | 2017 | Science China Chemistry2017，60，2： | 1 |
| 10 | Insights into human eNOS,nNOS and iNOS structures and medicinal indications from statistical analyses of their interactions with bound compounds显示文摘83 Structures of human nNOS,55 structures of human eNOS,13 structures of iNOS,and about 126 reported NOS-bound compounds are summarized and analyzed.Structural and statistical analysis show that,at least one copy of each analyzed compound binds to the active site(the substrate arginine binding site)of human NOS.And binding features of the three isoforms show differences,but the binding preference of compounds is not in the way helpful for inhibitor design targeting nNOS and iNOS,or for activator design targeting eNOS.This research shows that there is a strong structural and functional similarity between oxygenase domains of human NOS isoforms,especially the architecture,residue composition,size,shape,and distribution profile of hydrophobicity,polarity and charge of the active site.The selectivity and efficacy of inhibitors over the rest of isoforms rely a lot on chance and randomness.Further increase of selectivity via rational improvement is uncertain,unpredictable and unreliable,therefore,to achieve high selectivity through targeting this site is complicated and requires combinative investigation.After analysis on the current two targeting sites in NOS,the highly conserved arginine binding pocket and H4B binding pocket,new potential drug-targeting sites are proposed based on structure and sequence profiling.This comprehensive analysis on the structure and interaction profiles of human NOS and bound compounds provides fresh insights for drug discovery and pharmacological research,and the new discovery here is practically applied to guide protein-structure based drug discovery. | Jianshu Dong DiéLi Lei Kang Chenbing Luo Jiangyun Wang | 2023 | Biophysics Reports2023，9，3： | 0 |
| 11 | A genetically engineered Escherichia coli that senses and degrades tetracycline antibiotic residue显示文摘Due to the abuse of antibiotics,antibiotic residues can be detected in both natural environment and various industrial products,posing threat to the environment and human health.Here we describe the design and implementation of an engineered Escherichia coli capable of degrading tetracycline(Tc)-one of the commonly used antibiotics once on humans and now on poultry,cattle and fisheries.A Tcdegrading enzyme,TetX,from the obligate anaerobe Bacteroides fragilis was cloned and recombinantly expressed in E.coli and fully characterized,including its Km and kcat value.We quantitatively evaluated its activity both in vitro and in vivo by UVeVis spectrometer and LC-MS.Moreover,we used a tetracycline inducible amplification circuit including T7 RNA polymerase and its specific promoter PT7 to enhance the expression level of TetX,and studied the dose-response of TetX under different inducer concentrations.Since the deployment of genetically modified organisms(GMOs)outside laboratory brings about safety concerns,it is necessary to explore the possibility of integrating a kill-switch.Toxin-Antitoxin(TA)systems were used to construct a mutually dependent host-plasmid platform and biocontainment systems in various academic and industrious situations.We selected nine TA systems from various bacteria strains and measured the toxicity of toxins(T)and the detoxifying activity of cognate antitoxins(A)to validate their potential to be used to build a kill-switch.These results prove the possibility of using engineered microorganisms to tackle antibiotic residues in environment efficiently and safely. | Zepeng Mu Zhuoning Zou Ye Yang Wenbo Wang Yue Xu Jianyi Huang Ruiling Cai Ye Liu Yajin Mo Boyi Wang Yiqun Dang Yongming Li Yushan Liu Yueren Jiang Qingyang Tan Xiaohong Liu Cheng Hu Hua Li Sha Wei Chunbo Lou Yang Yu Jiangyun Wang | 2018 | Synthetic and Systems Biotechnology2018，3，3： | 0 |
| 12 | Significant expansion and red-shifting of fluorescent protein chromophore determined through computational design and genetic code expansion显示文摘Fluorescent proteins (FPs)with emission wavelengths in the far-red and infrared regions of the spectrum provide powerful tools for deep-tissue and super-resolution imaging.The development of red-shifted FPs has evoked widespread interest and continuous engineering efforts.In this article, based on a computational design and genetic code expansion,we report a rational approach to significantly expand and red-shift the chromophore of green fluorescent protein (GFP).We applied computational calculations to predict the excitation and emission wavelengths of a FP chromophore harboring unnatural amino acids (UAA)and identify in silico an appropriate UAA,2-amino-3-(6- hydroxynaphthalen-2-yl)propanoic acid (naphthol-Ala).Our methodology allowed us to formulate a GFP variant (cpsfGFP-66-Naphthol-Ala)with red-shifted absorbance and emission spectral maxima exceeding 60 and 130 nm,respectively,compared to those of GFP.The GFP chromophore is formed through autocatalytic post-translational modification to generate a planar 4-(p-hydroxybenzylidene)-5- imidazolinone chromophore.We solved the crystal structure of cpsfGFP-66-naphthol-Ala at 1.3 ■ resolution and demonstrated the formation of a much larger conjugated n-system when the phenol group is replaced by naphthol.These results explain the significant red-shifting of the excitation and emission spectra of cpsfGFP-66-naphthol-Ala. | Li Wang Xian Chen Xuzhen GUO liasong Li Qi Liu Fuying Kang Xudong Wang Cheng HU Haiping Liu Weimin Gong Wei Zhuang Xiaohong Liu Jiangyun Wang | 2018 | Biophysics Reports2018，4，5： | 0 |
| 13 | COVID-19 induces new-onset insulin resistance and lipid metabolic dysregulation via regulation of secreted metabolic factors显示文摘Abnormal glucose and lipid metabolism in COVID-19 patients were recently reported with unclear mechanism.In this study,we retrospectively investigated a cohort of COVID-19 patients without pre-existing metabolic-related diseases,and found new-onset in suli n resista nee,hyperglycemia,and decreased HDL-C in these patie nts.Mecha nistically,SARS-CoV-2 infecti on in creased the expression of RE1-silencing transcription factor(REST),which modulated the expression of secreted metabolic factors including myeloperoxidase,apelin,and myostatin at the transcriptional level,resulting in the perturbation of glucose and lipid metabolism.Furthermore,several lipids,including(±)5-HETE,(±)12-HETE,propionic acid,and isobutyric acid were identified as the potential biomarkers of COVID-19-induced metabolic dysregulation,especially in insulin resistance.Taken together,our study revealed insulin resistance as the direct cause of hyperglycemia upon COVID-19,and further illustrated the underlying mechanisms,providing potential therapeutic targets for COVID-19-induced metabolic complications. | Xi He Chenshu Liu Jiangyun Peng Zilun Li Fang Li Jian Wang Ao Hu Meixiu Peng Kan Huang Dongxiao Fan Na Li Fuchun Zhang Weiping Cai Xinghua Tan Zhongwei Hu Xilong Deng Yueping Li Xiaoneng Mo Linghua Li Yaling Shi Li Yang Yuanyuan Zhu Yanrong Wu Huichao Liang Baolin Liao Wenxin Hong Ruiying He Jiaojiao Li Pengle Guo Youguang Zhuo Lingzhai Zhao Fengyu Hu Wenxue Li Wei Zhu Zefeng Zhang Zeling Guo Wei Zhang Xiqiang Hong Wei kang Cai Lei Gu Ziming Du Yang Zhang Jin Xu Tao Zuo Kai Deng Li Yan Xinwen Chen Sifan Chen Chunliang Lei | 2022 | Signal Transduction and Targeted Therapy2022，7，1： | 0 |
| 14 | A genetically encoded sulfotyrosine for VHR function research显示文摘Dear Editor,Protein tyrosine phosphorylation is one of the most prevalent forms of post-translational modification and plays a signifi cant role in the control of a variety of cellular processes,including signal transduction,and is implicated in many human diseases(Eckhart et al.,1979;Karisch et al.,2011).The human vac-cinia H1-related phosphatase(VHR),the fi rst human dual specifi city protein phos-phatase(DSP),hydrolyzes phosphate monoesters from phosphotyrosine,phosphothreonine,or phosphoserine residues(Aroca et al.,1995).DSPs func-tion in signal transduction and in control of the cell cycle;more than 20 mamma-lian DSPs have so far been identifi ed.Although VHR can dephosphorylate both Erk and Jnk in vivo(Schumacher et al.,2002),it differs from other‘typi-cal’mitogen activated protein(MAP)kinase phosphatases(MKPs).With only 185 amino acids,VHR consists of only a catalytic domain with no recogniz-ing or docking domain. | Yueting Zheng Xiaoxuan Lv Jiangyun Wang | 2013 | Protein & Cell2013，4，10： | 0 |
| 15 | Research on system architecture of modeling and simulation显示文摘The modeling and simulation(M&S)architecture describes and defines the relationship between the different parts of a simulation.The simulation system architecture and simulation support platform architecture are discussed separately.The simulation support platform architecture consists of the management layer,the resource layer,the communication layer,the application layer and the infrastructure layer.The best way is to design and realize the M&S collaborative environment for simulation support platform in the resource-communication-application three-dimension space. | WANG Jiangyun WANG Xingren | 2007 | Frontiers of Electrical and Electronic Engineering in China2007，2，1： | 0 |
| 16 | Visual assessment of global chromatin intranuclear localization and its cellular diversification in mouse cells显示文摘DNA is the core that controls the life activities of cells.In eukaryotic cells,DNA is dispersedly packaged into multiple strands of chromatin to regulate cellular life activities;however,the underlying logic and significance of this configuration remain unclear.In mouse cells,pericentromeric satellite DNA from multiple strands of chromatin can aggregate to form chromocenters[1,2].Based on microscopic imaging techniques and analysis,we further reveal that there is nonrandom recombination aggregation between chromatin in different mouse nuclei,leading to differentiation of nuclear structure and correlation with cell function. | Yongqiang Wang Xinyi Yao Yuanyuan Xu Xu Cheng Jiangyun Peng Qimei Pan Kaishun Hu Lu Li Yin Zhang Dong Yin | 2023 | Acta Biochimica et Biophysica Sinica2023，55，3： | 0 |
| 17 | A convenient method for distinguishing human and mouse cells in situ显示文摘Animal models play critical roles in the field of biomedical research.Mouse models with transplanted human cells or tissues,such as cell line-derived xenografts(CDX)and patient-derived xenografts(PDX),have been widely used[1].Tracking transplanted cells and understanding the spatial relationship between donor and host are extremely important for the study of these models.To distinguish cell sources in mouse xenograft models at the morphological level,antibody recognition methods(immunohistochemistry and immunofluorescence),transgenic reporter methods,and chromosome-specific probe labeling methods are widely used[2–5].However,these methods all need to consider the issues of label specificity,signal intensity,in situ applicability,and complexity of experimental operations,which increase the difficulty in use.In this report,we described a simple method for accurately distinguishing human and mouse nuclei based on 4´,6-diamidino-2-phenylindole(DAPI)staining.This method can accurately identify species not only in cultured cells,but also in mouse xenograft models.Using short-wavelength excitation and high brightness of DAPI stains,this easy-to-use method is expected to be widely used in the field of biomedical research. | Yongqiang Wang Zixian Huang Kaishun Hu Jiangyun Peng Weicheng Yao Weixi Deng Jiyuan Zuo Yin Zhang Dong Yin | 2021 | Acta Biochimica et Biophysica Sinica2021，53，1： | 0 |
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