|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Efficient genome editing in plants using a CRISPR/Cas system显示文摘 | Zhengyan Feng Botao Zhang Wona Ding Xiaodong Liu Dong-Lei Yang Pengliang Wei Fengqiu Cao Shihua Zhu Feng Zhang Yanfei Mao Jian-Kang Zhu | 2013 | Cell Research2013，23，10： | 211 |
| 2 | Characteristics of climatic trends and correlation between pan-evaporation and environmental factors in the last 40 years over China显示文摘Using the data observed by 62 Chinese Routine Meteorological Stations (CRMS) with long term radiation observation, the climatic trends and the relationship between pan-evaporation and its environmental factors are analyzed comprehensively. The results show that during the last 40 years, the relative humidity is uptrend in west China, downtrend in east China, and their extrema are 0.20%/a and ?0.22%/a respectively; the precipitations of about 61% CRMS keep uptrend, its maximum can reach 10.52 mm/a2 while the cloud amounts of about 79% CRMS keep downtrend slightly. About 98% CRMS display the air temperature uptrend, and the maximum is 0.11℃/a. About 76% CRMS display the land surface temperature uptrend. About 87% CRMS show the daily range of temperature downtrend. The global radiations observed by about 85% CRMS and the 10 m wind speeds observed by about 77% CRMS hold downtrend. The annual pan-evaporations of about 66% CRMS hold descend trend, and the biggest descent reaches ?24.9 mm/a2. The pan-evaporation has good relationship with many environmental factors, but the relationship with the relative humidity is the best. All of the climatic trends respond to the global climate changes. | ZUOHongchao LIDongliang HUYinqiao BAOYan LüShihua | 2005 | Chinese Science Bulletin2005，50，12： | 37 |
| 3 | One-step generation of p53 gene biallelic mutant Cynomolgus monkey via the CRISPR/Cas system显示文摘 | Haifeng Wan Chunjing Feng Fei Teng Shihua Yang Baoyang Hu Yuyu Niu Andy Peng Xiang Weizhen Fang Weizhi Ji Wei Li Xiaoyang Zhao Qi Zhou | 2015 | Cell Research2015，25，2： | 39 |
| 4 | Drag Torque Prediction Model for the Wet Clutches显示文摘Reduction of drag torque in disengaged wet clutch is one of important potentials for vehicle transmission improvement. The flow of the oil film in clutch clearance is investigated. A three-dimension Navier-Stokes(N-S) equation based on laminar flow is presented to model the drag torque. Pressure and speed distribution in radial and circumferential directions are deduced. The theoretical analysis reveals that oil flow acceleration in radial direction caused by centrifugal force is the key reason for the shrinking of oil film as constant feeding flow rate. The peak drag torque occurs at the beginning of oil film shrinking. A variable is introduced to describe effective oil film area and drag torque after oil film shrinking is well evaluated with the variable. Under the working condition, tests were made to obtain drag torque curves at different clutch speed and oil viscosity. The tests confirm that simulation results agree with test data. The model performs well in the prediction of drag torque and lays a theoretical foundation to reduce it. | HU Jibin PENG Zengxiong YUAN Shihua | 2009 | Chinese Journal of Mechanical Engineering2009，22，2： | 26 |
| 5 | Stability analysis for a second-order continuous finite-time control system subject to a disturbance显示文摘In this paper, using finite-time control method, we consider the disturbance analysis of a second-order system with unknown but bounded disturbance. We show that the states of the second-order system will be stabilized to a region containing the origin. The radius of this region is determined by the control parameters and can be rendered as small as desired. The rigorous stability analysis is also given. Compared with the conventional PD control law, the finite-time control law yields a better disturbance rejection performance. Numerical simulation results show the effectiveness of the method. | Shihong DING Shihua LI Qi LI | 2009 | 控制理论与应用(英文版)2009，7，3： | 25 |
| 6 | Experimental Research and Mathematical Model of Drag Torque in Single-plate Wet Clutch显示文摘Reduction of drag torque is one of important potentials to improve transmission efficiency.Existing mathematical model of drag torque was not accurate to predict the decrease after oil film shrinking because of the difficulty in modeling the flow pattern between two plates.Flow pattern was considered as laminar flow and full oil film in the gap between two plates in traditional model.Subsequent equivalent circumferential degree model presented an improvement in oil film shrinking due to centrifugal force,but was also based on full oil film in the gap,which resulted difference between model prediction and experimental data.The objective of this paper is to develop an accurate mathematical model for the above problem by using experimental verification.An experimental apparatus was set up to test drag torque of disengaged wet clutch consisting of single friction and separate plate.A high speed camera was used to record the flow pattern through transparent quartz disk plate.The visualization of flow pattern in the clearance was investigated to evaluate the characteristics of oil film shrinking.Visual test results reveal that the oil film begins to shrink from outer radius to inner radius at the stationary plate and only flows along the rotating plate after shrinking.Meanwhile,drag torque decreases sharply due to little contact area between the stationary plate and the oil.A three-dimensional Navier-Stokes (N-S) equation based on laminar flow is presented to model the drag torque.Pressure distributions in radial and circumferential directions as well as speed distributions are deduced.The model analysis reveals that the acceleration of flow in radial direction caused by centrifugal force is the key reason for the shrinking at the constant feeding flow rate.An approach to descript flow pattern was presented on the basis of visual observation.The drag torque predicted by the model agrees well with test data for non-grooved wet clutch.The proposed model enhances the precision for predicting drag torque,and lays down a framework on which some subsequent models are developed. | YUAN Shihua PENG Zengxiong JING Chongbo | 2011 | Chinese Journal of Mechanical Engineering2011，24，1： | 25 |
| 7 | Characteristics on Hydro-mechanical Transmission in Power Shift Process显示文摘To improve the vehicular power and acceleration performance and reduce the shift impact, the study of the characteristics on power shift is necessary.Based on the flexible hydraulic unit of hydro-mechanical transmission, this paper explores the feasibility of shift without power interruption.With the four models concerning displacement ratio, rotational speed, rotational torque and power at ideal shift point, the characteristics on power shift in different running conditions are analyzed, and the rules of power shift are revealed.The theoretical analysis and test results show that the hydro-mechanical transmission can shift without power interruption in different running conditions.Furthermore, there exists an ideal shift point in theory, at which point the cycle power in hydro-mechanical transmission can't be generated, and the impact on the system can be reduced to the minimum.However, if before or after this ideal shift point, a cycle power can be generated. | HU Jibin WEI Chao YUAN Shihua JING Chongbo | 2009 | Chinese Journal of Mechanical Engineering2009，22，1： | 21 |
| 8 | 从可持续发展战略看炫耀性消费显示文摘本文从探索炫耀性消费的理论源头出发,重点分析了炫耀性消费的负面效应。提出,根据我国国情,我们必须 选择可持续的消费模式,坚持走可持续发展道路。 | 李时华 龚志民 Gong Zhimini Li Shihua | 2005 | 消费经济2005，21，1： | 18 |
| 9 | A Chromosome-Scale Genome Assembly of Paper Mulberry(Bmussonetia papyrifera)Provides New Insights into Its Forage and Papermaking Usage显示文摘Paper mulberry(Broussonetia papyrifera)is a well-known woody tree historically used for Cai Lun papermaking,one of the four great inventions of ancient China.More recently,Paper mulberry has also been used as forage to address the shortage of feedstuff because of its digestible crude fiber and high protein contents.In this study,we obtained a chromosome-scale genome assembly for Paper mulberry using integrated approaches,including Illumina and PacBio sequencing platform as well as Hi-C,optical,and genetic maps.The assembled Paper mulberry genome consists of 386.83 Mb,which is close to the estimated size,and 99.25%(383.93 Mb)of the assembly was assigned to 13 pseudochromosomes.Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families,respectively,accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.Moreover,the increased ratio of syringyl-lignin to guaiacyl-lignin in Paper mulberry underscores its suitability for use in medicine,forage,papermaking,and barkcloth making.We also identified the rootassociated microbiota of Paper mulberry and found that Pseudomonas and Rhizobia were enriched in its roots and may provide the source of nitrogen for its stems and leaves via symbiotic nitrogen fixation.Collectively,these results suggest that Paper mulberry might have undergone adaptive evolution and recruited nitrogen-fixing microbes to promote growth by enhancing flavonoid production and altering lignin monomer composition.Our study provides significant insights into genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making,and as forage.These insights will facilitate further domestication and selection as well as industrial utilization of Paper mulberry worldwide. | Xianjun Peng Hui Liu Peilin Chen Feng Tang Yanmin Hu Fenfen Wang Zhi Pi Meiling Zhao Naizhi Chen Hui Chen Xiaokang Zhang Xueqing Yan Min Liu Xiaojun Fu Guofeng Zhao Pu Yao Lili Wang He Dai Xuming Li Wei Xiong Wencai Xu Hongkun Zheng Haiyan Yu Shihua Shen | 2019 | Molecular Plant2019，12，5： | 18 |
| 10 | Simulation of rainfall-runoff and watershed convergence process in the upper reaches of Heihe River Basin,July 2002显示文摘The watershed flow concentration scheme in the distributed hydrology-soil- vegetation model (DHSVM) is coupled with the mesoscale atmospheric model MM5 version 3.5, in which the Oregen States University land surface model (OSULSM) was involved. The flood event which happened in July 2002 in the upper reaches of Heihe river basin is simulated and the surface flow convergence process is shown with this coupled model. It has been concluded that times water head reaches each place of the basin are different. Water amount at each point is split-flow proportionally as the drops in elevation between it and neighbor points. Large part of the water amount pass away in greater slope direction and small part pass away in smaller slope one.Adding of the slope convergence makes the atmospheric model redistributes the surface water laterally. | GAO Yanhong L(U) Shihua CHENG Guodong | 2004 | Science China Earth Sciences2004，47，z1： | 13 |
| 11 | Effect of aeration rate on composting of penicillin mycelial dreg显示文摘Pilot scale experiments with forced aeration were conducted to estimate effects of aeration rates on the performance of composting penicillin mycelial dreg using sewage sludge as inoculation. Three aeration rates of 0.15, 0.50 and 0.90 L/(min·kg) organic matter(OM) were examined. The principal physicochemical parameters were monitored during the 32 day composting period. Results showed that the higher aeration rate of 0.90 L/(min·kg) did not corresponded to a longer thermophilic duration and higher rates of OM degradation;but the lower aeration rate of 0.15 L/(min·kg) did induce an accumulation of NH+4-N contents due to the inhibition of nitrification. On the other hand, aeration rate has little effect on degradation of penicillin. The results show that the longest phase of thermophilic temperatures ≥ 55°C, the maximum NO-3-N content and seed germination, and the minimum C/N ratio were obtained with 0.50 L/(min·kg) OM. Therefore, aeration rates of0.50 L/(min·kg) OM can be recommended for composting penicillin mycelial dreg. | Zhiqiang Chen Shihua Zhang Qinxue Wen Jun Zheng | 2015 | Journal of Environmental Sciences2015，27，11： | 13 |
| 12 | TALEN-based generation of a cynomolgus monkey disease model for human microcephaly显示文摘基因在非人类的首领编辑可以为在人探索病原学和遗传上基于的神经病学的混乱的治疗学的策略导致珍贵模型。然而,仔细在人模仿病理学、行为的赤字的神经病学的混乱的一个猴子模型成功地还没被产生了。Microcephalin 1 (MCPH1 ) 在人的大脑,和智力迟钝伴随的 MCPH1 变化原因头小畸型的进化被含有。这里,我们产生了用抄写带 biallelic MCPH1 变化的一只 cynomolgus 猴子(Macaca fascicularis ) 像使活跃之物的受动器核酸酶。猴子概括了大多数在病人观察的重要临床的特征,包括在头圆周,早熟的染色体冷凝作用(PCC ) ,语料库 callosum 的发育不全和上面的手足 spasticity 的显著减小。而且,在变异的真皮的成纤维细胞的 MCPH1 的 overexpression 救了 PCC 症候群。这个猴子模型可以帮助我们阐明在人的头小畸型并且更好的致病的 MCPH1 的角色在首领大脑尺寸的进化理解这蛋白质的功能。 | Qiong Ke Weiqiang Li Xingqiang Lai Hong Chen Lihua Huang Zhuang Kang Kai Li Jie Ren Xiaofeng Lin Haiqing Zheng Weijun Huang Yunhan Ma Dongdong Xu Zheng Chen Xinming Song Xinyi Lin Min Zhuang Tao Wang Fengfeng Zhuang Jianzhong Xi Frank Fuxiang Mao Huimin Xia Bruce T Lahn Qi Zhou Shihua Yang Andy Peng Xiang | 2016 | Cell Research2016，26，9： | 13 |
| 13 | Species-dependent neuropathology in transgenic SOD' aigs显示文摘 | Huaqiang Yang Guohao Wang Haitao Sun Runzhe Shu Tao Liu Chuan-En Wang Zhaoming Liu Yu Zhao Bentian Zhao Zhen Ouyang Dongshan Yang Jiao Huang Yueling Zhou Shihua Li Xiaodan Jiang Zhicheng Xiao Xiao-Jiang Li Liangxue Lai | 2014 | Cell Research2014，24，4： | 13 |
| 14 | Improving CLM4.5 Simulations of Land–Atmosphere Exchange during Freeze–Thaw Processes on the Tibetan Plateau显示文摘Soil is heterogeneous and has different thermal and hydraulic properties, causing varied behavior in heat and moisture transport. Therefore, soil has an important effect on land–atmosphere interactions. In this study, an improved soil parameterization scheme that considers gravel and organic matter in the soil was introduced into CLM4.5(Community Land Model). By using data from the Zoige and Madoi sites on the Tibetan Plateau, the ability of the model to simultaneously simulate the duration of freeze–thaw periods, soil temperature, soil moisture, and surface energy during freeze–thaw processes, was validated. The results indicated that:(1) the new parameterization performed better in simulating the duration of the frozen, thawing, unfrozen, and freezing periods;(2) with the new scheme, the soil thermal conductivity values were decreased;(3) the new parameterization improved soil temperature simulation and effectively decreased cold biases;(4) the new parameterization scheme effectively decreased the dry biases of soil liquid water content during the freezing, completely frozen, and thawing periods, but increased the wet biases during the completely thawed period; and(5) the net radiation, latent heat flux, and soil surface heat flux of the Zoige and Madoi sites were much improved by the new organic matter and thermal conductivity parameterization. | Siqiong LUO Xuewei FANG Shihua LYU Yu ZHANG Boli CHEN | 2017 | Journal of Meteorological Research2017，31，5： | 13 |
| 15 | Adaptive fault-tolerant controller design for airbreathing hypersonic vehicle with input saturation显示文摘The problem of fault-tolerant control is discussed for the longitudinal model of an airbreathing hypersonic vehicle(AHV) with actuator faults and external disturbances.Firstly,a fault-tolerant control strategy is presented for the longitudinal model of an AHV,which guarantees that velocity and altitude track their reference trajectories at an exponential convergence rate.However,this method needs to know the minimum value of the actuator efficiency factor and the upper bound of the external disturbances,which makes it not easy to implement.Then an improved adaptive fault-tolerant control scheme is proposed,where two adaptive laws are employed to estimate the upper bound of the external disturbances and the minimum value of the actuator efficiency factor,respectively.Secondly,the problem of designing a control scheme with control constraints is further considered,and a new adaptive fault-tolerant control strategy with input saturation is designed to guarantee that velocity and altitude track their reference trajectories.Finally,simulation results are given to show the effectiveness of the proposed methods. | Haibin Sun Shihua Li Changyin Sun | 2013 | Journal of Systems Engineering and Electronics2013，24，3： | 12 |
| 16 | Mesenchymal stem cells and immune disorders: from basic science to clinical transition显示文摘As a promising candidate seed cell type in regenerative medicine, mesenchymal stem cells (MSCs) have attracted considerable attention. The unique capacity of MSCs to exert a regulatory effect on immunity in an autologous/allergenic manner makes them an attractive therapeutic cell type for immune disorders. In this review, we discussed the current knowledge of and advances in MSCs, including its basic biological properties, i.e., multilineage differentiation, secretome, and immunomodulation. Specifically, on the basis of our previous work, we proposed three new concepts of MSCs, i.e.,“subtotipotent stem cell” hypothesis, MSC system, and “Yin and Yang” balance of MSC regulation, which may bring new insights into our understanding of MSCs. Furthermore, we analyzed data from the Clinical Trials database (http://gffzzd3f38a9638994581hfcpnnw0f96oc6o9v.ffgz.tsg.suse.edu.cn) on registered clinical trials using MSCs to treat a variety of immune diseases, such as graft-versus-host disease, systemic lupus erythematosus, and multiple sclerosis. In addition, we highlighted MSC clinical trials in China and discussed the challenges and future directions in the field of MSC clinical application. | Shihua Wang Rongjia Zhu Hongling Li Jing Li Qin Han Robert Chunhua Zhao | 2019 | Frontiers of Medicine2019，13，2： | 12 |
| 17 | CRISPR/Cas9-mediated PINK1 deletion leads to neurodegeneration in rhesus monkeys显示文摘Dear Editor,PINK1 mutations cause autosomal recessive and early-onset Parkinson’s disease(PD)with selective neurodegeneration.Unfortunately,current PINK1 knockout(KO)mouse1,2 and pig models3,4 are unable to recapitulate the selective and overt neurodegeneration seen in PD patients.Furthermore,endogenous Pink1 in the mouse brain is expressed at very low levels and can only be detected via immunoprecipitation,5 meaning that PINK1’s function in the mammalian brain needs to be assessed using larger animals that are closer to humans.We previously used CRISPR/Cas9 to target the monkey gene in one-cell stage embryos.6 Using the same approach,we designed two gRNAs to target exon 2(T1)and exon 4(T2),which encode a kinase domain in the PINK1 gene of rhesus monkeys(Fig.1a).CRISPR/Cas9 and PINK1 gRNAs were injected into one-cell stage rhesus monkey embryos.A T7E1 assay and sequencing of PCR products from the injected embryos showed high efficiency(61.5%)in targeting PINK1(Fig.1b and Supplementary information,Fig.S1).Transfer of 87 embryos to 28 surrogate rhesus monkeys resulted in 11 pregnancies(39.2%)(Fig.1b).Eleven fetuses developed to term and were born naturally.Of these live monkeys,eight carried PINK1 mutations(M),and three were wild type(WT).However,three mutant monkeys(M1,M3 and M4)were newborn triplets that struggled to survive and died 3-4 days after birth.One WT newborn monkey also died after a difficult labor.Another mutant monkey(M2)died 7 days after birth without noticeable warning signs or symptoms.The other three mutant monkeys(M6,M7 and M8)have lived for three years;M5,however,reduced its food intake and showed weakness at the age of 1.5 years,and died 30 days after anesthesia for MRI examination. | Weili Yang Yunbo Liu Zhuchi Tu Chong Xiao Sen Yan Xishan Ma Xiangyu Guo Xiusheng Chen Peng Yin Zhengyi Yang Su Yang Tianzi Jiang Shihua Li Chuan Qin Xiao-Jiang Li | 2019 | Cell Research2019，29，4： | 12 |
| 18 | Simulation of oasis breeze circulation in the arid region of the Northwestern China显示文摘 | LU Shihua, AN Xingqin & CHEN YuchunCold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China | 2004 | Science China Earth Sciences2004，47，z1： | 12 |
| 19 | Synthesis of Branched Chains with Actuation Redundancy for Eliminating Interior Singularities of 3T1R Parallel Mechanisms显示文摘Although it is common to eliminate the singularity of parallel mechanism by adding the branched chain with actuation redundancy, there is no theory and method for the configuration synthesis of the branched chain with actuation redundancy in parallel mechanism. Branched chains with actuation redundancy are synthesized for eliminating interior singularity of 3-translational and 1-rotational(3T1R) parallel mechanisms. Guided by the discriminance method of hybrid screw group according to Grassmann line geometry, all the possibilities are listed for the occurrence of interior singularities in 3T1R parallel mechanism. Based on the linear relevance of screw system and the principles of eliminating parallel mechanism singularity with actuation redundancy, different types of branched chains with actuation redundancy are synthesized systematically to indicate the layout and the number of the branched chainsinterior with actuation redundancy. A general method is proposed for the configuration synthesis of the branched chains with actuation redundancy of the redundant parallel mechanism, and it builds a solid foundation for the subsequent performance optimization of the redundant actuation parallel mechanism. | LI Shihua LIU Yanmin CUI Hongliu NIU Yunzhan ZHAO Yanzhi | 2016 | Chinese Journal of Mechanical Engineering2016，29，2： | 11 |
| 20 | Estimation of Turbulent Fluxes Using the Flux-Variance Method over an Alpine Meadow Surface in the Eastern Tibetan Plateau显示文摘流动变化类似关系和数量的垂直转移在表面的不同类型上展出不同,导致在估计用流动变化方法的狂暴的流动的数量之中的相对运输效率的不同 parameterization 途径。我们调查了在雨季期间在中间的水文学条件下面在夏天在东方西藏的高原在一块开、同类、扁平的草地上使用 eddycovariance 大小的这些问题。处于不稳定的条件,温度,水蒸汽,和 CO2 跟随了流动变化类似关系,但是没出现在精确一样的方法由于不同角色(活跃或被动) 这些数量。温度,水蒸汽和 CO2 的类似常数被发现分别地是 1.12, 1.19 和 1.17。热交通比水蒸汽和 CO2 更有效。基于估计的理智的热流动,到水蒸汽和 CO2 的热的相对运输效率的五个 parameterization 方法被检验估计潜伏的热和 CO2 流动。流动变化类似关系的本地决心的策略为潜伏的热和 CO2 流动的评价被推荐。这条途径对代表平均相对运输效率更好,并且对技术上更容易适用,与另外的更复杂的相比。 | WANG Shaoying ZHANG Yu LU Shihua LIU Heping SHANG Lunyu | 2013 | Advances in Atmospheric Sciences2013，30，2： | 11 |
您所在的机构暂未开通此部分的功能，请联系管理员