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| 1 | One-step generation of p53 gene biallelic mutant Cynomolgus monkey via the CRISPR/Cas system显示文摘 | Haifeng Wan Chunjing Feng Fei Teng Shihua Yang Baoyang Hu Yuyu Niu Andy Peng Xiang Weizhen Fang Weizhi Ji Wei Li Xiaoyang Zhao Qi Zhou | 2015 | Cell Research2015，25，2： | 39 |
| 2 | Atg7 is required for acrosome biogenesis during spermatogenesis in mice显示文摘acrosome 是盖住精子原子核的前面的部分并且在授精的过程起一个必要作用的一个专业化细胞器。位于这个 lysosome 相关的细胞器(LRO ) 的生物的续生说下面的分子的机制大部分仍然是未知的。这里,我们显示出房间特定的 Atg7 大美人老鼠是的那个细菌由于在 acrosome 生物的续生说的一个缺点不肥沃并且显示了类似于人的 globozoospermia 的显型;这个繁殖缺点被 intracytoplasmic 精子注射成功地救。而且,在细菌房间的 Atg7 的弄空没影响细菌房间的开发的早阶段,但是精子发生在以后上演,在 Golgi 期间没有通过到保险丝进一个单个 acrosomal 泡的 proacrosomal 泡分阶段执行,它最后导致了不规则或将近 round-headed 精子。Autophagic 流动在弄空 Atg7 的细菌房间被破坏,最后导致 LC3 变化形式的失败到 Golgi 导出仪器的泡。另外, Atg7 部分调整了另一 globozoospermia 相关的蛋白质,联系 Golgi 的 PDZ 卷和卷卷包含主题的蛋白质(GOPC ) ,在 acrosome 生物的续生说期间。最后,任何一个 autophagy 的注射或进睾丸的 lysosome 禁止者导致了类似的显型到细菌的房间特定的 Atg7 大美人老鼠。总的来说,我们的结果揭开为在 acrosome 的生物的续生说的 Atg7 的一个新角色,并且我们提供证据为 acrosome 支持 autolysosome 起源假设。 | Hongna Wang Haifeng Wan Xixia Li Weixiao Liu Qi Chen Yaqing Wang Lin Yang Hongmei Tang Xiujun Zhang Enkui Duan Xiaoyang Zhao Fei Gao Wei Li | 2014 | Cell Research2014，24，7： | 25 |
| 3 | Birth of fertile bimaternal offspring following intracytoplasmic injection of parthenogenetic haploid embryonic stem cells显示文摘 | Zhikun Li Haifeng Wan Guihai Feng Leyun Wang Zhengquan He YukaiWang Xiu-Jie Wang Wei Li Qi Zhou Baoyang Hu | 2016 | Cell Research2016，26，1： | 15 |
| 4 | Parthenogenetic haploid embryonic stem cells produce fertile mice显示文摘 | Haifeng Wan Zhengquan He Mingzhu Dong Tiantian Gu Guan-Zheng Luo Fei Teng Baolong Xia Wei Li Chunjing Feng Xin Li Tianda Li Ling Shua Rui Fu Liu Wang Xiu-Jie Wang Xiao-Yang Zhao Qi Zhou | 2013 | Cell Research2013，23，11： | 13 |
| 5 | Successful generation of cloned mice using nuclear transfer from induced pluripotent stem cells显示文摘 | Shuya Zhou Chenhui Ding Xiaoyang Zhao Eryao Wang Xiangpeng Dai Lei Liu Wei Li Zichuan Liu Haifeng Wan Chunjing Feng Tang Hai Liu Wang Qi Zhou | 2010 | Cell Research2010，20，7： | 10 |
| 6 | Generation of fertile offspring from Kitw/Kitwv mice through differentiation of gene corrected nuclear transfer embryonic stem cells显示文摘 | Yan Yuan Quan Zhou Haifeng Wan Bin Shen Xuepeng Wang Mei Wang Chunjing Feng Mingming Xie Tiantian Gu Tao Zhou Rui Fu Xingxu Huang Qi Zhou Jiahao Sha Xiao-Yang Zhao | 2015 | Cell Research2015，25，7： | 7 |
| 7 | Domesticated cynomolgus monkey embryonic stem cells allow the generation of neonatal interspecies chimeric pigs显示文摘Blastocyst complementation by pluripotent stem cell(PSC)injection is believed to be the most promising method to generate xenogeneic organs.However,ethical issues prevent the study of human chimeras in the late embryonic stage of development.Primate embryonic stem cells(ESCs),which have similar pluripotency to human ESCs,are a good model for studying interspecies chimerism and organ generation.However,whether primate ESCs can be used in xenogenous grafts remains unclear.In this study,we evaluated the chimeric ability of cynomolgus monkey(Macaca fascicularis)ESCs(cmESCs)in pigs,which are excellent hosts because of their many similarities to humans.We report an optimized culture medium that enhanced the anti-apoptotic ability of cmESCs and improved the development of chimeric embryos,in which domesticated cmESCs(D-ESCs)injected into pig blastocysts differentiated into cells of all three germ layers.In addition,we obtained two neonatal interspecies chimeras,in which we observed tissue-specific D-ESC differentiation.Taken together,the results demonstrate the capability of D-ESCs to integrate and differentiate into functional cells in a porcine model,with a chimeric ratio of 0.001-0.0001 in different neonate tissues.We believe this work will facilitate future developments in xenogeneic organogenesis,bringing us one step closer to producing tissue-specific functional cells and organs in a large animal model through interspecies blastocyst complementation. | Rui Fu Dawei Yu Jilong Ren Chongyang Li Jing Wang Guihai Feng Xuepeng Wang Haifeng Wan Tianda Li Libin Wang Ying Zhang Tang Hai Wei Li Qi Zhou | 2020 | Protein & Cell2020，11，2： | 4 |
| 8 | Transfer of β-hydroxy-β-methylbutyrate from sows to their offspring and its impact on muscle fiber type transformation and performance in pigs显示文摘Background: Previous studies suggested that supplementation of lactating sows with β-hydroxy-β-methylbutyrate(HMB) could improve the performance of weaning pigs, but there were little information in the muscle fiber type transformation of the offspring and the subsequent performance in pigs from weaning through finishing in response to maternal HMB consumption. The purpose of this study was to determine the effect of supplementing lactating sows with HMB on skeletal muscle fiber type transformation and growth of the offspring during d 28 and180 after birth. A total of 20 sows according to their body weight were divided into the control(CON, n = 10) or HMB groups(HMB, n = 10). Sows in the HMB group were supplemented with β-hydroxy-β-methylbutyrate calcium(HMB-Ca) 2 g/kg feed during d 1 to 27 of lactation. After weaning, 48 mixed sex piglets were blocked by sow treatment and fed standard diets for post-weaning, growing, finishing periods. Growth performance was recorded during d 28 to 180 after birth. Pigs were slaughtered on d 28(n = 6/treatment) and 180(n = 6/treatment) postnatal and the longissimus dorsi(LD) was collected, respectively.Results: The HMB-fed sows during lactation showed increased HMB concentration(P < 0.05) in milk and LD of weaning piglets(P < 0.05). In addition, offsprings in HMB group had a higher finishing BW and lean percentage than did pigs in CON group(P < 0.05), meanwhile, compared with pigs from sows fed the CON diet, pigs from sows fed HMB diet showed higher type Ⅱ muscle fiber cross-sectional area(CSA), elevated myosin heavy chain(MyHC) Ⅱb and Sox6 mRNA, and fast-MyHC protein levels in LD(P < 0.05).Conclusions: HMB supplemented to sow diets throughout lactation increases the levels of HMB in maternal milk and skeletal muscle of pigs during d 28 after birth and promotes subsequent performance of pigs between d 28 and 180 of age by enhancing glycolytic muscle fiber transformation. | Haifeng Wan Jiatao Zhu Caimei Wu Pan Zhou Yong Shen Yan Lin Shengyu Xu Lianqiang Che Bin Feng Jian Li Zhengfeng Fang De Wu | 2017 | Journal of Animal Science and Biotechnology2017，8，2： | 2 |
| 9 | Mouse cloning and somatic cell reprogramming using electrofused blastomeres显示文摘老鼠从受精卵克隆能为遗传上定制的人的胚胎的茎(ES ) 的生产帮助途径的开发没被卵母细胞可获得性的限制抑制的房间线。然而,成功地从导致 ES 房间线的严重地区分的体的房间施主被用作标明日期仅仅接合子原子核的接受者。在富饶诊所,先进胚胎的阶段的胚胎通常被存储为支持经由体的原子转移的房间线还没被证明了的 ES 的推导的未来使用,而是他们的能力。这里,我们报导那只二房间的阶段 electrofused 老鼠胚胎,在有丝分裂逮捕了,能从从分裂球到体的房间的施主房间支持原子核的发展 reprogramming。从胚胎的施主的实时、完整术语的克隆的小狗,以及从胚胎或体的施主的 pluripotent ES 房间线,成功地从这些重建的胚胎被产生。先进阶段培植前胚胎是不能的通常发展在 electrofusion 以后称为并且一个体的房间原子核转,显示丢弃的培植前人胚胎能是为最小化为人的治疗学的克隆的道德的担心的研究的一个重要资源。我们的途径提供一种吸引人、实际的选择给为病人特定的人的 ES 房间线的产生的用捐赠卵母细胞的治疗学的克隆。 | Amjad Riaz Xiaoyang Zha Xiangpeng Dai Wei Li Lei Liu Haifeng Wan Yang Yu Liu Wang Qi Zhou | 2011 | Cell Research2011，21，5： | 2 |
| 10 | Synthesis and ordered aggregation in water of a blue light-emitting PEO-PHP-PEO triblock oligomer显示文摘A novel blue light-emitting coil-rod-coil triblock oligomer, PEO-PHP-PEO, which consists of para-hexaphenyl (PHP) as the rigid rod and poly(ethylene oxide) (PEO, M w = 750) as the flexible coil, has been synthesized. It is the first soluble poly(para-phenylene) molecule containing no lateral substituent, which can dissolve not only in organic solvents such as chloroform and THF, but also in water. The critical micelle concentration (CMC) of PEO-PHP-PEO in water is 5.25×10-4 mol·L-1 and the surface tension at CMC, 鉉MC, is 61.29 mN·m-1. The absorption peak of the molecularly dissolved PEO-PHP-PEO in water is centered at 323 nm and the fluorescence spectrum has a maximum at 432 nm and shows the vibronic fine structure typical of molecular dissolution. The photoluminescence spectrum of the aggregate solution is similar to that of the PHP polycrystal film, indicating that the ordered packing of PHP block has taken place due to a combination of hydrophobic interaction and p-p stacking. The oligomer self-assembles into cotton-like nanofibers with diameter of about 20 nm in 5.0×10-3 mol·L-1 aqueous solution. | HE Haifeng, CAO Hongqing, WAN Xinhua, TU Yingfeng, CHEN Xiaofang & ZHOU Qifeng Department of Polymer Science and Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China Correspondence should be addressed to Wan Xinhua (e-mail: xhwan@pku.edu.cn) | 2003 | Chinese Science Bulletin2003，48，15： | 2 |
| 11 | Self-organization of unimolecular micelles in beam stream for functional mesoporous metal oxide nanofibers显示文摘The use of linear amphiphilic block copolymers as templates is an important method for the preparation of mesoporous materials.However,the obtained assemblies are usually sensitive to synthetic conditions,which impedes the preparation of such mesoporous materials in certain environments.Herein,we report a universal strategy applying an amphiphilic multiarm triblock copolymer in the preparation of mesoporous metal oxide nanofibers(NFs)using one metal oxide(TiO_(2),ZrO_(2),WO_(3),CeO_(2)),or two(TiO_(2)/WO 3,TiO_(2)/ZrO_(2),TiO_(2)/CeO_(2))and three(TiO_(2)/WO_(3)/CuO)metal oxides as composites.The template consists of modified β-cyclodextrin as the center of the macromolecule which is attached sequentially to a block of polystyrene,poly(acrylic acid),and poly(ethylene oxide).Under electrospinning conditions,stable unimolecular micelles are formed and effectively co-assemble with metal ions to form fibrous nanostructures.As indicated by various characterization methods,the synthesized TiO_(2) and its derived composite NFs maintain a straight and continuous fibrous structure after calcination,and TiO_(2) NFs exhibit uniform mesopores of 10.8 nm in diameter and a large Brunauer-Emmett-Teller surface area of 143.3 m^(2)g^(−1).Benefiting from the characteristic structure,still present after modification,Pt-decorated mesoporous TiO_(2) NFs display excellent ability in the visible-light photocatalytic degradation of tetracycline,which is superior to the commercial P25 catalyst.This study reveals a promising strategy for the preparation of fibrous mesoporous metal oxides. | Chongfei Gu Xiaoshan Fan Guihua Zhu Yuchi Fan Haifeng Wang Tao Zhao Qi Xiao Yuan Fang Xiaopeng Li Wan Jiang Lianjun Wang Pengpeng Qiu Wei Luo | 2022 | Fundamental Research2022，2，5： | 1 |
| 12 | Research on fault pat- tern recognition for aircraft fuel system with its performance sim- ulation 显示文摘 | Wang Haifeng Song Bifeng Wan Fangyi | 2011 | IEEE Computer Society2011，，： | 1 |
| 13 | RNA Guided Genome Editing in Mouse Germ-Line Stem Cells显示文摘Spermatogonial stem cells(SSCs)reside on the basement membrane of the seminiferous tubules in mammalian testes(Nagano et al.,1998).After isolation and purification of SSCs from mouse testis,SSCs can be cultured in vitro to derive germ-line stem cells(GSCs)which have the ability of proliferation over 2 years(Kanatsu-Shinohara et al.,2003;KanatsuShinohara and Shinohara,2007).GSCs can be localized in the seminiferous tubules to re-establish the spermatogenesis | Xuepeng Wang Yan Yuan Quan Zhou Haifeng Wan Mei Wang Qi Zhou Xiao-Yang Zhao Jiahao Sha | 2014 | Journal of Genetics and Genomics2014，41，7： | 1 |
| 14 | SYNTHESIS AND CHARACTERIZATION OF A NOVEL BLUE LIGHT EMITTING ROD-COIL DIBLOCK OLIGOMER显示文摘Four new rod-coil diblock molecules, which had well-defined para-phenylene oligomers (PPP, from biphenyl to quinquephenyl) as rigid segments and poly(ethylene oxide) (PEO, Mw ca. 750) as flexible segments, were synthesized. The chemical structure and the number of repeating unit in PEO coil and PPP rod were characterized by 1H-NMR and MALDITOF mass spectrometry. The diblock oligomers showed good solubility in common organic solvents and aggregated in water as characterized by a blue shift in UV-vis absorption spectra, a red shift in emission spectra, quenching of the fluorescence and formation of the microfibrilla and fractal-like structures. The formation of different supramolecular structures meant that this effort might lead to commercial important highly organized functional materials. | Lifang Yang Haifeng He Hongqing Cao Xinhua Wan Qi-feng Zhou Department of Polymer Science & Engineering College of Chemistry & Molecular Engineering Peking University Beijing 100871, China | 2002 | Chinese Journal of Polymer Science2002，20，5： | 1 |
| 15 | Generation of tetraploid complementation mice from embryonic stem cells cultured with chemical defined medium显示文摘Mouse embryonic stem cells(mESCs)derived from inner cell mass(ICM)of pre-implantation embryos,can maintain undifferentiated state when cultured in N2B27 medium supplemented with GSK3inhibitor CHIR99021 and MEK inhibitor PD0325901(‘‘2i’’)and leukemia inhibitor factor(LIF).Compare to conventional culture medium,all components of this medium are defined.With the N2B27 medium,‘‘2i’’and LIF,mESCs can contribute to the germline of the chimeric embryos,however,whether the‘‘all-ES cells’’mice can been generated by tetraploid complementation is unclear yet,while the tetraploid complementation serve as a golden standard to assess the pluripotency of ES cells.Here,our study showed that mESCs derived and cultured with the N2B27 complete medium could generate fertile mice by tetraploid complementation.In addition,the survival rate of tetraploid complementation mice produced by inbred mES cell lines is higher than the conventional culture condition,and increased the percentage of Oct4 positive cells contrast to conventional medium either.Therefore,the N2B27 medium supplemented with‘‘2i’’and LIF is an alternative choice forthe derivation and long-term culture of mouse embryonic stem cells. | Chunjing Feng Haifeng Wan Xiao-Yang Zhao Liu Wang Qi Zhou | 2014 | Chinese Science Bulletin2014，59，22： | 1 |
| 16 | 'Host-Guest'chemistry in the synthesis of ordered nonsiliceous mesoporous materials显示文摘 | WAN YING YANG HAIFENG ZHAO DONGYUAN | 2006 | Accounts of Chemical Reaserch2006，39，： | 1 |
| 17 | Durable pluripotency and haploidy in epiblast stem cells derived from haploid embryonic stem cells in vitro显示文摘Haploid pluripotent stem cells,such as haploid embryonic stem cells(haESCs),facilitate the genetic study of recessive traits.In vitro,fish haESCs maintain haploidy in both undifferentiated and differentiated states,but whether mammalian haESCs can preserve pluripotency in the haploid state has not been tested.Here,wereport thatmousehaESCs can differentiate in vitro into haploid epiblast stem cells(haEpiSCs),which maintain an intact haploid genome,unlimited self-renewal potential,and durable pluripotency to differentiate into various tissues in vitro and in vivo.Mechanistically,the maintenance of self-renewal potential depends on the Activin/bFGF pathway.We further show that haEpiSCs can differentiate in vitro into haploid progenitor-like cells.When injected into the cytoplasm of an oocyte,androgenetic haEpiSC(ahaEpiSCs)can support embryonic development until midgestation(E12.5).Together,these resultsdemonstrate durable pluripotency inmousehaESCs andhaEpiSCs,aswell asthe valuable potential of using these haploid pluripotent stem cells in high-throughput genetic screening. | Ling Shuai Yukai Wang Mingzhu Dong Xuepeng Wang Lisi Sang Mei Wang Haifeng Wan Guanzheng Luo Tiantian Gu Yan Yuan Chunjing Feng Fei Teng Wei Li Xiuying Liu Tianda Li Liu Wang Xiu-Jie Wang Xiao-Yang Zhao Qi Zhou | 2015 | Journal of Molecular Cell Biology2015，7，4： | 1 |
| 18 | Genetic Modification and Screening in Rat Using Haploid Embryonic Stem Cells显示文摘 | Wei Li Xin Li Tianda Li Ming-Gui Jiang Haifeng Wan Guan-Zheng Luo Chunjing Feng Xiaolong Cui Fei Teng Yan Yuan Quan Zhou Qi Gu Ling Shuai Jiahao Sha Yamei Xiao Liu Wang Zhonghua Liu Xiu-Jie Wang Xiao-Yang Zhao Qi Zhou | 2013 | Cell Stem Cell2013，，： | 1 |
| 19 | miR-205 promotes tumor proliferation and invasion through targetingESRRG in endometrial carcinoma显示文摘 | Ning Su Haifeng Qiu Yifei Chen Tingting Yang Qin Yan Xiaoping Wan | 2013 | Oncology Reports2013，，： | 1 |
| 20 | New classification-oriented laparoscopic anatomical hepatectomy strategy for hepatocellular carcinoma invading two or more(sub)segments in the left lobe显示文摘With technological advances in laparoscopic hepatectomy and new navigation devices,such as flexible laparoscopic ultrasound probes,indocyanine green(ICG)-fluorescent imaging and three-dimensional imaging,laparoscopic segmentectomy(LS),which maximizes the preservation of the functional hepatic reserve and the possibility for future repeat hepatectomy while ensuring adequate surgical margins,has become a feasible alternative to hemihepatectomy for addressing hepatocellular carcinoma(HCC). | Haifeng Wan Tian Lan Ting Duan Kunlin Xie Jiwei Huang Jiayin Yang Yong Zeng Li Jiang Hong Wu | 2022 | Chinese Medical Journal2022，135，21： | 0 |
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