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| 1 | Subcurative radiation significantly increases cell proliferation, invasion, and migration of primary glioblastoma multiforme in vivo显示文摘Tumor cell proliferation, infiltration, migration, and neovascularization are known causes of treatment resistance in glioblastoma multiforme(GBM). The purpose of this study was to determine the effect of radiation on the growth characteristics of primary human GBM developed in a nude rat. Primary GBM cells grown from explanted GBM tissues were implanted orthotopically in nude rats. Tumor growth was confirmed by magnetic resonance imaging on day 77(baseline) after implantation. The rats underwent irradiation to a dose of 50 Gy delivered subcuratively on day 84 postimplantation(n = 8), or underwent no radiation(n = 8). Brain tissues were obtained on day 112(nonirradiated) or day 133(irradiated). Immunohistochemistry was performed to determine tumor cell proliferation(Ki-67) and to assess the expression of infiltration marker(matrix metalloproteinase-2, MMP-2) and cell migration marker(CD44). Tumor neovascularization was assessed by microvessel density using von-Willebrand factor(vWF) staining. Magnetic resonance imaging showed well-developed, infiltrative tumors in 11 weeks postimplantation. The proportion of Ki-67-positive cells in tumors undergoing radiation was(71 ± 15)% compared with(25 ± 12)% in the nonirradiated group(P = 0.02). The number of MMP-2-positive areas and proportion of CD44-positive cells were also high in tumors receiving radiation, indicating great invasion and infiltration. Microvessel density analysis did not show a significant difference between nonirradiated and irradiated tumors. Taken together, we found that subcurative radiation significantly increased proliferation, invasion, and migration of primary GBM. Our study provides insights into possible mechanisms of treatment resistance following radiation therapy for GBM. | Adarsh Shankar Sanath Kumar Asm Iskander Nadimpalli RS Varma Branislava Janic Ana deCarvalho Tom Mikkelsen Joseph A Frank Meser M Ali Robert A Knight Stephen Brown Ali S Arbab | 2014 | Chinese Journal of Cancer2014，33，3： | 5 |
| 2 | Total hip arthroplasty in fused hips with spine stiffness in ankylosing spondylitis显示文摘Ankylosing spondylitis(AS)is characterized by involvement of the spine and hip joints with progressive stiffness and loss of function.Functional impairment is significant,with spine and hip involvement,and is predominantly seen in the younger age group.Total hip arthroplasty(THA)for fused hips with stiff spines in AS results in considerable improvement of mobility and function.Spine stiffness associated with AS needs evaluation before THA.Preoperative assessment with lateral spine radiographs shows loss of lumbar lordosis.Spinopelvic mobility is reduced with change in sacral slope from sitting to standing less than 10 degrees conforming to the stiff pattern.Care should be taken to reduce acetabular component anteversion at THA in these fused hips,as the posterior pelvic tilt would increase the risk of posterior impingement and anterior dislocation.Fused hips require femoral neck osteotomy,true acetabular floor identification and restoration of the hip center with horizontal and vertical offset to achieve a good functional outcome.Cementless and cemented fixation have shown comparable long-term results with the choice dependent on bone stock at THA.Risks at THA in AS include intraoperative fractures,dislocation,heterotopic ossification,among others.There is significant improvement of functional scores and quality of life following THA in these deserving young individuals with fused hips and spine stiffness. | Anil Thomas Oommen Triplicane Dwarakanathan Hariharan Viruthipadavil John Chandy Pradeep MathewPoonnoose Arun Shankar A Roncy Savio Kuruvilla Jozy Timothy | 2021 | World Journal of Orthopedics2021，12，12： | 2 |
| 3 | 在有煽动性、非煽动性的腹的疼痛显型的孩子的 interleukin-6 和桅杆房间的反的关系显示文摘 AIM: To investigate interleukin-6 (IL-6), mast cells, enterochromaffin cells, 5-hydroxytryptamine, and substance P in the gastrointestinal mucosa of children with abdominal pain. METHODS: Formalin-fixed paraffin-embedded gas-trointestinal biopsy blocks from patients (n = 48) with non-inflammatory bowel disease (irritable bowel syndrome and functional abdominal pain) and inflammatory bowel disease were sectioned and stained for IL-6, mast cells, enterochromaffin cells, 5-hydroxytryptamine, and substance P. All children had chronic abdominal pain as part of their presenting symptoms. Biopsy phenotype was confirmed by a pathologist, blinded to patient information. Descriptive statistics, chi-square, and independent sample t tests were used to compare differences between the inflammatory and non-inflammatory groups. RESULTS: The cohort (n = 48), mean age 11.9 years (SD = 2.9), 54.2% females, 90% Caucasian, was comprised of a non-inflammatory (n = 26) and an inflammatory (n = 22) phenotype. There was a significant negative correlation between substance P expression and mast cell count (P = 0.05, r = -0.373). Substance P was found to be expressed more often in female patient biopsies and more intensely in the upper gastrointestinal mucosa as compared to the lower mucosa. There were significantly increased gastrointestinal mucosal immunoreactivity to IL-6 (P = 0.004) in the inflammatory phenotype compared to non-inflammatory. Additionally, we found significantly increased mast cells (P = 0.049) in the mucosa of the non-inflammatory phenotype compared to the inflammatory group. This difference was particularly noted in the lower colon biopsies. CONCLUSION: The findings of this study yield preliminary evidence in identifying biomarkers of undiagnosed abdominal pain in children and may suggest candidate genes for future evaluation. | Wendy A Henderson Ravi Shankar Tara J Taylor Arseima Y Del Valle-Pinero David E Kleiner Kevin H Kim Nader N Youssef | 2012 | World Journal of Gastrointestinal Pathophysiology2012，3，6： | 2 |
| 4 | Therapeutic applications of moloclonal antibodies 显示文摘 | Berger M Shankar V Vafai A | 2002 | Am J Med Sci2002，324，1： | 1 |
| 5 | Characterization of microstructures in Inconel 625 using X-ray diffraction peak broad- ening and lattice parameter measurements显示文摘 | RAI S K KUMAR A SHANKAR V | 2004 | Scripta Materialia2004，51，1： | 1 |
| 6 | Markers of inflammation predict the long-term risk of developing chronic kidney disease:a population-based cohort study 显示文摘 | Shankar A Sun L Klein EB | 2011 | Kidney Int2011，80，： | 1 |
| 7 | Infection- derived Enterocoecusfaecalis strain mrfare enriched in esp, a gene encoding a novel surface protein显示文摘 | Shankar V Baghdayan A S Huyeke M M | 1999 | Infect Immun1999，67，： | 1 |
| 8 | The association between se- rum uric acid level and long-term incidence of hypertension: Popu- lation-based cohort study显示文摘 | Shankar A Klein R Klein BE | 2006 | J Hum Hypertens2006，20，12： | 1 |
| 9 | Transaction processing in replicated data in the DDBMS显示文摘 | Srivastava A Shankar U Tiwari S K | 2012 | International Journal of Modern Engineering Research2012，2，4： | 1 |
| 10 | Nitric oxide inhibits wound collagen synthesis显示文摘 | Rasik AM Shankar R | 1999 | Mol Cell Biochem1999，200，： | 1 |
| 11 | Pathogenic enterocoeci: new developments in the 21st century显示文摘 | Tendolkar P M Baghdayan A S Shankar N | 2003 | Cell Mol Life Sci2003，60，12： | 1 |
| 12 | Dynamical Behavior of Ratc-Bascd Flow Control Mechanisms显示文摘 | J C Bolot A U Shankar | 1990 | ACM SIGCOMM Computer Communication Review1990，20，4： | 1 |
| 13 | Role of Enterococcus faecalis surface protein Esp in the pathogenesis of ascending urinary tract infection显示文摘 | Shankar N Lockatell C V Baghdayan A S | 2001 | Infect Immun2001，69，： | 1 |
| 14 | Association between plasma ho- mocysteine and microalbuminuria in persons without hyperten- sion,diabetes mellitus, and cardiovascular disease显示文摘 | Sabanayagam C Shankar A | 2011 | Clin Exp Nephrol2011，15，1： | 1 |
| 15 | Effect of pellet basicity and MgO content on the quality and microstructure of hematite pellets显示文摘 | DWARAPUDI S GHOSH T K SHANKAR A | 2011 | International Journal of Mineral Processing2011，99，1234： | 1 |
| 16 | Surface electrocardio- gram to predict outcome in candidates for cardiac resynchroniza- tion therapy: a sub-analysis of the CARE-HF trial显示文摘 | Gervais R Leclercq C Shankar A | 2009 | Eur J Heart Fail2009，11，： | 1 |
| 17 | Association between serum gamma-glutamyltransferase level and prehypertension among US adults显示文摘 | Shankar A Li J | 2007 | Circ J2007，71，10： | 1 |
| 18 | The N-terminal domain of enterococcal surface protein, Esp, is sufficient for Esp mediated biofilm enhancement in Enterococcusfaecalis 显示文摘 | Tendolkar P M Baghdayan A S Shankar N | 2005 | JBacteriol2005，187，： | 1 |
| 19 | Effect of pellet basicity and MgO content on the quality and microstructure of hematite pellets显示文摘 | Dwarapud S Tamal K Shankar A | 2011 | Mineral Processing2011，99，： | 1 |
| 20 | The association between acculturation and hypertension in a multiethnic sample of US adults显示文摘 | Teppala S Shankar A Ducatman A | 2010 | J Am Soc Hypertens2010，4，5： | 1 |
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