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| 1 | A Simple Approach to Bioconjugation at Diverse Levels: Metal-Free Click Reactions of Activated Alkynes with Native Groups of Biotargets without Prefunctionalization显示文摘Te efcient bioconjugation of functional groups/molecules to targeted matrix and bio-related species drives the great development of material science and biomedicine,while the dilemma of metal catalysis,uneasy premodifcation,and limited reaction efciency in traditional bioconjugation has restricted the booming development to some extent.Here,we provide a strategy for metal-free click bioconjugation at diverse levels based on activated alkynes.As a proof-of-concept,the abundant native groups including amine,thiol,and hydroxyl groups can directly react with activated alkynes without any modifcation in the absence of metal catalysis.Trough this strategy,high-efcient modifcation and potential functionalization can be achieved for natural polysaccharide,biocompatible polyethylene glycol(PEG),synthetic polymers,cell penetrating peptide,protein,fast whole-cell mapping,and even quick diferentiation and staining of Gram-positive bacteria,etc.Terefore,current metal-free click bioconjugation strategy based on activated alkynes is promising for the development of quick fuorescence labeling and functional modifcation of many targets and can be widely applied towards the fabrication of complex biomaterials and future in vivo labeling and detection. | Xianglong Hu Xueqian Zhao Benzhao He Zheng Zhao Zheng Zheng Pengfei Zhang Xiujuan Shi Ryan T.K.Kwok Jacky W.Y.Lam Anjun Qin Ben Zhong Tang | 2018 | Research2018，，1： | 7 |
| 2 | Sensitivity of transvaginal ultrasound screening for endometrial cancer in postmenopausal women: a case-control study within the UKCTOCS cohort显示文摘 | Ian Jacobs Aleksandra Gentry-Maharaj Matthew Burnell Ranjit Manchanda Naveena Singh Aarti Sharma Andy Ryan Mourad W Seif Nazar N Amso Gillian Turner Carol Brunell Gwendolen Fletcher Rani Rangar Kathy Ford Keith Godfrey Alberto Lopes David Oram Jonathan He | 2011 | Lancet Oncology2011，，1： | 2 |
| 3 | MMP-2 and MMP-9 secretion by rpe is stimulated by angiogenic molecules found in choroidal neovascular membranes 显示文摘 | Hoffmann S He S Ehren M Ryan S J Wiedemann P Hinton DR | 2006 | Retina2006，26，4： | 1 |
| 4 | Raclmediated mitochondrial H2O2 generation regulates MMP-9 gene expression in macrophages via inhibition of SP-1 and AP-1显示文摘 | Murthy S Ryan A He C | | 0，，32： | 1 |
| 5 | Promotion of adhesion and migration of RPE cells to provisional extracellular matrices by TNF-alpha显示文摘 | Jin M He S Worpel V Ryan SJ Hinton DR | 2000 | Invest Ophthalmol Vis Sci2000，41，13： | 1 |
| 6 | Hypoxia-inducible factor 1α is essential for cell cycle arrest during hypoxia显示文摘 | Goda N Ryan HE Khadivi B | | 0，，1： | 1 |
| 7 | Temperature and soil organic matter decomposition rates-synthesis of current knowledge and a way forward显示文摘 | Conant RT Ryan MG Agren GI Birge HE Davidson EA Eliasson PE Evans SE Frey SD Giardina CP Hopkins FM Hyvǒnen R Kirschbaum MUF Lavallee JM Leifeld J Parton WJ Megan Steinweg J Wallenstein MD Martin Wetterstedt JA Bradford MA | | 0，，： | 1 |
| 8 | Gene expression profiling of the hypoxia signaling pathway in hypoxia-inducible factor lalpha null mouse embryonic fibroblasts 显示文摘 | Vengellur A Woods BG Ryan HE | 2003 | Gene Expr2003，11，34： | 1 |
| 9 | Lab-in-cell based on spontaneous amino-yne click polymerization显示文摘Unnatural reaction in the living cells is a powerful tool for biological research. However, the polymerization inside cells is rarely reported. In this work, a lab-in-cell is illustrated based on our developed spontaneous amino-yne click polymerization. Carbonyl group activated terminal diyne can spontaneously polymerize with tetraphenylethene(TPE)-containing primary diamine inside cells, and polymer with weight-average molecular weight of 7,300 was yielded. By utilizing this in vivo amino-yne click polymerization and taking advantage of the aggregation-induced emission feature of TPE, a 'turn-on' cell imaging was realized,and in-situ killing of cells was also acquired by destroying the structures of actin and tubulin, which cannot be realized by preprepared polymer. This strategy provides a useful platform and holds great promise in biochemistry and therapy applications. | Rong Hu Xu Chen Taotao Zhou Han Si Benzhao He Ryan T. K. Kwok Anjun Qin Ben Zhong Tang | 2019 | Science China Chemistry2019，62，9： | 1 |
| 10 | Hypoxia- inducible factor- 1alpha is essential for cell cycle arrest during hypoxia显示文摘 | Goda N Ryan HE Khadivi B | 2003 | Mol Cell Biol2003，23，1： | 1 |
| 11 | Racl-mediated mitochondrial H2 O2 Generation regulates MMP-9 gene expression in mac- rophages via inhibition of SP-1 and AP-1显示文摘 | Murthy S Ryan A He C | 2010 | J Biol Chem2010，285，25： | 1 |
| 12 | Coordinate up-regulation of hypoxia inducible factor (HIF)-la and HIF-1 target genes during multi-stage epidermal carcinogenesis and wound healing显示文摘 | Elson DA Ryan HE Snow JW | | Cancer Res0，2，： | 1 |
| 13 | HIF-1 alpha is required for solid tumor formation and embryonic vascularization显示文摘 | Ryan HE Lo J Johnson RS | 1998 | EMBO J1998，17，11： | 1 |
| 14 | Hypoxia-inducible factor-1α is a positive factor in solid tumor growth显示文摘 | Ryan HE Poloni M McNuhy W | 2000 | Cancer Res2000，60，15： | 1 |
| 15 | Hypoxia -inducible factor-1 alpha is a positive factor in solid tumor growth显示文摘 | Ryan HE Poloni M McNulty W | 2000 | Cancer Res2000，60，15： | 1 |
| 16 | Hypoxia-inducible factor-1α is a positive factor in solid tumor growth 显示文摘 | Ryan HE Poloni M McNulty W | 2000 | Cancer Res2000，60，15： | 1 |
| 17 | HIF- 1α is required for solid tumor formation and embryonic vascularization 显示文摘 | Ryan HE Lo J Johnson RS | 1998 | EMBO J1998，17，： | 1 |
| 18 | Hypoxia in cartilage:HIF-1 is essential for chondrocyte growth arrest and survival显示文摘 | Schipani E Ryan HE Didrickson S | 2001 | Genes Dev2001，15，21： | 1 |
| 19 | Hypoxia-inducible factor-1 alpha is a positive factor in solid tumor growth显示文摘 | Ryan HE Poloni M McNuhy W | 2000 | Cancer Res2000，60，15： | 1 |
| 20 | Hypoxia-inducible factor-l alpha is a positive factor in solid tumor growth显示文摘 | Ryan HE Poloni M McNulty W | 2000 | Cancer Res2000，60，15： | 1 |
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