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| 1 | Mesenchymal stem cells: a new strategy for immunosuppression and tissue repair显示文摘间充质的干细胞(MSC ) 为治疗各种各样的疾病有大潜力,特别那些与织物有关损坏包含有免疫力的反应。各种各样的研究证明了 MSC 在 vitro 并且在 vivo 是强烈抑制免疫力的。我们的最近的研究证明了那未刺激的 MSC 确实不能免疫力的抑制;他们与 TNF- 伪, IL-1 伪或 IL-1 尾与激活的淋巴细胞的上层清液,或与 IFN- 纬的联合在刺激之上变得 potently 抑制免疫力。这观察表明在某些情形下面,煽动性的 cytokines 能实际上变得抑制免疫力。我们证明在调停 MSC 的免疫力的抑制的机制有一个种类变化:由告知 cytokine 的老鼠 MSC 的免疫力的抑制被氮的氧化物调停(没有) 而由告知 cytokine 的人的 MSC 的免疫力的抑制通过 indoleamine 被执行 2, 3-dioxygenase (伊多语) 。在有煽动性的 cytokines 的刺激之上,另外,老鼠和人的 MSC 分泌几白血球 chemokines 显然服务与 MSC 吸引有免疫力的房间进最近,在哪儿不或伊多语被预言很活跃。因此,由煽动性的刺激 cytokine 的 MSC 的免疫力的抑制经由 chemokines 的一致行动发生并且免疫者禁止没有或伊多语由 MSC 生产了。因此,我们的结果在对待有免疫力的回答导致的织物损害关于调停 MSC 的免疫力的抑制并且为 MSC 的更好的申请的机制提供新奇信息。 | Yufang Shi Gangzheng Hu Juanjuan Su Wenzhao Li Qing Chen Peishun Shou Chunliang Xu Xiaodong Chen Yin Huang Zhexin Zhu Xin Huang Xiaoyan Han Ningxia Xie Guangwen Ren | 2010 | Cell Research2010，20，5： | 72 |
| 2 | Zircon U-Pb age and Hf isotope of Quanyishang A-type granite in Yichang: Signification for the Yangtze continental cratonization in Paleoproterozoic显示文摘Zircon U-Pb age and Hf isotope, and major and trace element compositions were reported for granite at Quanyishang, which intruded into the Kongling complex in Yichang, Hubei Province. The results show that the Quanyishang granite is rich in silicon and alkalis but poor in calcium and magnesium, and displays enrichment in Ga, Y, Zr, Nb but depletion in Sr and Ba, exhibiting the post-orogenic A-type affinity. 90% zircons from the granite are concordant, and give a middle Paleoproterozoic magmatic crystallization age (mean 1854 Ma). Initial Hf isotope ratios (176Hf/177Hf)i of the middle Paleoproterozoic zircons range from 0.280863 to 0.281134 and they have negative εHf(t) values with a minimum of -26.3. These zircons give the depleted mantle model ages (TDM) of 2.9―3.3 Ga (mean 3.0 Ga), and the average crustal model ages (Tcrust) of 3.6―4.2 Ga (mean 3.8 Ga). A Mesoarchean grain with 207Pb/206Pb age of 2859 Ma has a slightly high TDM (3.4 Ga) but similar Tcrust (3.8 Ga) to the Paleoproterozoic zircons. All these data suggest that the source materials of the Quanyishang A-type granite are unusually old, at least ≥2.9 Ga (even Eoarchean). The event of crustal remelting, which resulted in the formation of the Quanyishang granite in the middle Paleoproterozoic, recorded the cratonization of the Yangtze conti- nent. The process may have relation to the extension and collapse of the deep crust with Archean ages, in response to the transition stage of the assembly and breakup of the Columbia supercontinent. | XIONG Qing HENG JianPing YU ChunMei SU YuPing TANG HuaYun ZHANG ZhiHai | 2009 | Chinese Science Bulletin2009，54，3： | 46 |
| 3 | Thyroid Nodules in Type 2 Diabetes Mellitus显示文摘The present analysis aims to investigate the prevalence of thyroid nodules in type 2 diabetes mellitus (T2DM) population.We searched PubMed,EMBASE,and Web of Science from inception to the March 1,2018.The studies were selected to estimate the prevalence of thyroid nodules in T2DM subjects and to compare the prevalence of thyroid nodules in different glucose tolerance status.The random effects model was used,and the outcome was presented as a pooled prevalence proportion with 95% confidence interval (95% CI) or a summary odds ratio (OR) with 95% CI.In the end,9 studies met the inclusion criteria and were included in the analysis.The pooled prevalence of thyroid nodules was 60%(95% CI:0.52,0.68) for T2DM 2 diabetes patients,50%(95% CI:0.4& 0.51) for pre-diabetes,and 43%(95% CI:0.34,0.52) for normal glucose tolerance population.Compared with patients without diabetes,diabetes subjects are more likely to develop thyroid nodules,adjusted OR for thyroid nodule was 1.78 (95% CI:1.25,2.55).Insulin resistance might be involved in thyroid nodule development. | Hong-mei ZHANG Qi-wen FENG Yi-xin NIU Qing SU Xia WANG | 2019 | Current Medical Science2019，39，4： | 19 |
| 4 | Knockdown of survivin expression by siRNAs enhances chemosensitivity of prostate cancer cells and attenuates its tumorigenicity显示文摘Survivin, apoptosis 家庭蛋白质的禁止者的一个成员,为肿瘤房间生存能力在肿瘤房间和它的重要角色由于它的选择表示在癌症成为了一个吸引人的治疗学的目标。这里,我们在前列腺癌症房间显示出那基于向量的小介入 RNA (siRNAs ) silenced survivin 表情,导致显著地减少了房间增长并且提高了 apoptosis,并且增加了前列腺癌症房间(PC-3 ) 的敏感到导致 apoptosis 代理人, platinol。而且,有表示 siRNA 向量的 PC-3 房间 transfected 在 vivo 在裸体老鼠异种皮移植显示出更低的肿瘤形成。这些结果证明由 siRNA 的 survivin 表示的抑制稀释了前列腺癌症房间的恶意的显型,并且可以为雄激素无关的前列腺癌症的基因治疗提供一条新奇途径。 | Jianjun Shen Jiayun Liu Yin Long Yinye Miao Mingquan Su Qing Zhang Hua Han Xiaoke Hao | 2009 | Acta Biochimica et Biophysica Sinica2009，41，3： | 19 |
| 5 | Research advances in traditional Chinese medicine syndromes in cancer patients显示文摘Traditional Chinese medicine(TCM)syndrome,also known as TCM ZHENG or TCM pattern,is an integral and essential part of TCM theory that helps to guide the design of individualized treatments.A TCM syndrome,in essence,is a characteristic profi le of all clinical manifestations in one patient that can be readily identifi ed by a TCM practitioner.In this article,the authors reviewed the presentations of TCM syndromes in seven common malignancies(liver,lung,gastric,breast,colorectal,pancreatic and esophageal cancers),the objectivity and the standardization of TCM syndrome differentiation,the evaluation of TCM syndrome modeling in cancer research,and syndrome differentiation-guided TCM treatment of cancers.A better understanding of TCM syndrome theory,as well as its potential biological basis,may contribute greatly to the clinical TCM diagnosis and the treatment of cancer. | Qing Ji Yun-quan Luo Wen-hai Wang Xuan Liu Qi Li Shi-bing Su | 2016 | Journal of Integrative Medicine2016，14，1： | 17 |
| 6 | A Randomized, Double-Blind, Multicenter, Placebo-Controlled Trial of Qi-Zhi-Wei-Tong Granules on Postprandial Distress Syndrome-Predominant Functional Dyspepsia显示文摘 | Qing Su Sheng-Liang Chen Hua-Hong Wang Lie-Xin Liang Ning Dai Bin Lyu Jun Zhang Rong-Quan Wang Ya-Li Zhang Yue Yu Jin-Song Liu Xiao-Hua Hou | 2018 | Chinese Medical Journal2018，，13： | 16 |
| 7 | A Chromosome-Level Genome Assembly of Garlic (Allium sativum) Provides Insights into Genome Evolution and Allicin Biosynthesis显示文摘Garlic,an economically important vegetable,spice,and medicinal crop,produces highly enlarged bulbs and unique organosulfur compounds.Here,we report a chromosome-level genome assembly for garlic,with a total size of approximately 16.24 Gb,as well as the annotation of 57561 predicted protein-coding genes,making garlic the first Allium species with a sequenced genome.Analysis of this garlic genome assembly reveals a recent burst of transposable elements,explaining the substantial expansion of the garlic genome.We examined the evolution of certain genes associated with the biosynthesis of allicin and inulin neoseries-type fructans,and provided new insights into the biosynthesis of these two compounds.Furthermore,a large-scale transcriptome was produced to characterize the expression patterns of garlic genes in different tissues and at various growth stages of enlarged bulbs.The reference genome and large-scale transcriptome data generated in this study provide valuable new resources for research on garlic biology and breeding. | Xiudong Sun Siyuan Zhu Ningyang Li Yi Cheng Jing Zhao Xuguang Qiao Li Lu Shiqi Liu Yanzhou Wang Chan Liu Benping Li Wu Guo Shuang Gao Zemao Yang Fu Li Zheng Zeng Qing Tang Yupeng Pan Mengjiao Guan Jian Zhao Xiaomi ng Lu Huanwe n Meng Zhenlin Han Chun she ng Gao Wenkai Jiang Xing Zhao Shilin Tian Jianguang Su Zhihui Cheng Touming Liu | 2020 | Molecular Plant2020，13，9： | 14 |
| 8 | Combined administration of anisodamine and neostigmine produces anti-shock effects: involvement of a7 nicotinic acetylcholine receptors显示文摘 | Li SUN Gu-fang ZHANG Xin ZHANG Qing LIU Jian-guo LIU Ding-feng SU Chong LIU | 2012 | Acta Pharmacologica Sinica2012，33，6： | 14 |
| 9 | Baicalein ameliorates ulcerative colitis by improving intestinal epithelial barrier via AhR/IL-22 pathway in ILC3s显示文摘Ulcerative colitis(UC)is a chronic inflammatory disease of the gastrointestinal tract,which is closely related to gut barrier dysfunction.Emerging evidence shows that interleukin-22(IL-22)derived from group 3 innate lymphoid cells(ILC3s)confers benefits on intestinal barrier,and IL-22 expression is controlled by aryl hydrocarbon receptor(AhR).Previous studies show that baicalein protects the colon from inflammatory damage.In this study we elucidated the molecular mechanisms underlying the protective effect of baicalein on intestinal barrier function in colitis mice.Mice were administered baicalein(10,20,40 mg·kg^(-1)·d^(-1),i.g.)for 10 days;the mice freely drank 3%dextran sulfate sodium(DSS)on D1-D7 to induce colitis.We showed that baicalein administration simultaneously ameliorated gut inflammation,decreased intestinal permeability,restored tight junctions of colons possibly via promoting AhR/IL-22 pathway.Co-administration of AhR antagonist CH223191(10 mg/kg,i.p.)partially blocked the therapeutic effects of baicalein in colitis mice,whereas AhR agonist FICZ(1μg,i.p.)ameliorated symptoms and gut barrier function in colitis mice.In a murine lymphocyte line MNK-3,baicalein(5-20μM)dose-dependently increased the expression of AhR downstream target protein CYP1A1,and enhanced IL-22 production through facilitating AhR nuclear translocation,these effects were greatly diminished in shAhR-MNK3 cells,suggesting that baicalein induced IL-22 production in AhR-dependent manner.To further clarify that,we constructed an in vitro system consisting of MNK-3 and Caco-2 cells,in which MNK-3 cell supernatant treated with baicalein could decrease FITC-dextran permeability and promoted the expression of tight junction proteins ZO-1 and occluding in Caco-2 cells.In conclusion,this study demonstrates that baicalein ameliorates colitis by improving intestinal epithelial barrier via AhR/IL-22 pathway in ILC3s,thus providing a potential therapy for UC. | Yan-yang Li Xiao-jing Wang Yu-lin Su Qing Wang Shao-wei Huang Zeng-feng Pan Yan-ping Chen Jun-jie Liang Mei-ling Zhang Xue-qian Xie Zhi-yun Wu Jin-yan Chen Lian Zhou Xia Luo | 2022 | Acta Pharmacologica Sinica2022，43，6： | 14 |
| 10 | Prevalence and characteristics of MAFLD in Chinese adults aged 40 years or older:A community-based study显示文摘Background: Nonalcoholic fatty liver disease(NAFLD) was recently proposed to be renamed metabolic dysfunction-associated fatty liver disease(MAFLD) with the diagnostic criteria revised. We investigated the similarities and differences in the prevalence and clinical characteristics of MAFLD and NAFLD in Chinese adults. Methods: A cross-sectional study of 9980 Chinese individuals aged 40 years or older was performed between 2011 and 2012 using randomized, stratifed cluster sampling in Shanghai, China. A detailed questionnaire and the results of abdominal ultrasonography, a standardized 2-h 75-g oral glucose tolerance test and blood biochemical examinations were collected. Results: A total of 9927 subjects were included in this study. The prevalence of MAFLD(40.3%) was significantly higher than that of NAFLD(36.9%)( P < 0.05). MAFLD was highly prevalent in type 2 diabetes mellitus(T2DM)(53.8%), impaired fasting glucose(35.7%) and impaired glucose tolerance(40.9%). High risk of advanced fbrosis based on fbrosis-4 was highly prevalent(14.7%) in lean MAFLD with T2DM. Among 9927 subjects, 3481(35.1%) fulflled the diagnostic criteria for MAFLD and NAFLD(MAFLD + NAFLD +), 521(5.2%) MAFLD + NAFLD-, and 181(1.8%) MAFLD-NAFLD +. The MAFLD + NAFLD-group had more signifcant metabolic disorders than those in the MAFLD + NAFLD + group(all P < 0.05). Among MAFLD-NAFLD + subjects, 82.9% had metabolic disorders. Conclusions: The new defnition of MAFLD may better reflect the pathogenesis related to metabolism. Future research should focus on studying the natural history, pathogenesis and treatment effectivity of the overlap and non-overlap of NAFLD and MAFLD subjects. | Jing Zeng Li Qin Qian Jin Rui-Xu Yang Guang Ning Qing Su Zhen Yang Jian-Gao Fan | 2022 | Hepatobiliary & Pancreatic Diseases International2022，21，2： | 13 |
| 11 | Impact of cigarette smoking on recurrence of hyperlipidemic acute pancreatitis显示文摘AIM To investigate the impact of cigarette smoking on the recurrence rate and recurrence-free survival in patients with hyperlipidemic acute pancreatitis(HLAP).METHODS A total of 863 patients were admitted to our hospital for acute pancreatitis(AP) from January 2013 to March2016, of whom 88 diagnosed with HLAP were enrolled in this retrospective study. Demographic data, medical history, previous episodes of pancreatitis, consumption of alcohol and cigarettes, as well as biochemical and hematological data were carefully recorded for univariate and multivariate analyses. During followup, the information on current smoking status and recurrent AP was gathered. Recurrence-free survival(RFS) was calculated using the Kaplan-Meier method, and the differences between groups were compared using the log-rank test.RESULTS No significant differences were observed between the three groups in age or medical history of hyperlipidemia, fatty liver, diabetes mellitus, hypertension, or AP. The current smokers had a remarkably higher recurrence rate and a greater incidence of repeated episodes of AP(50.0% and 77.8%, respectively) than non-smokers(9.8% and 39.0%), and these two percentages were reduced to 9.1% and 36.4% for patients who gave up smoking. The median follow-up time was 13.5 mo and HLAP recurred after hospital discharge in 23(26.1%) patients. Multivariate analysis identified current smoking(HR = 6.3, P = 0.020) as an independent risk factor contributing to HLAP recurrence. Current smokers had significantly worse RFS than non-smokers(23 mo vs 42 mo), but no significant difference was documented between ex-smokers(34 mo) and non-smokers. The RFS was not significantly different between light and heavy smokers.CONCLUSION Smoking is associated with worse RFS and an increased rate of HLAP recurrence. Continued smoking correlates with a compromised survival and smoking cessation should be recommended. | Jun-Xi Xiang Liang-Shuo Hu Peng Liu Bo-Yan Tian Qing Su Yi-Chun Ji Xu-Feng Zhang Xue-Min Liu Zheng Wu Yi Lv | 2017 | World Journal of Gastroenterology2017，23，47： | 11 |
| 12 | Transient expression and antigenic characterization of HBsAg of HBV nt551 A to G mutant显示文摘NTRODUCTIONFromthelate80s,therehavebeenincreasingnumberofreportsonhepatitisB(HB)patientswithatypicalHBVserologicalmarkers,so... | FANG De Xing 1, LI Fa Qing 1, TAN Wei Guo 1, CHEN Hua Biao 1, JIN Hui Ying 1, LI Su Qin 1, LIN Hou Ji 2 and ZHOU Zhen Xian 2 | 1999 | World Journal of Gastroenterology1999，5，1： | 10 |
| 13 | The Ghd7 transcription factor represses ARE1 expression to enhance nitrogen utilization and grain yield in rice显示文摘The genetic improvement of nitrogen use efficiency(NUE)of crops is vital for grain productivity and sustainable agriculture.However,the regulatory mechanism of NUE remains largely elusive.Here,we report that the rice Grain number,plant height,and heading date7(Ghd7)gene genetically acts upstream of ABC1 REPRESSOR1(ARE1),a negative regulator of NUE,to positively regulate nitrogen utilization.As a transcriptional repressor,Ghd7 directly binds to two Evening Element-like motifs in the promoter and intron 1 of ARE1,likely in a cooperative manner,to repress its expression.Ghd7 and ARE1 display diurnal expression patterns in an inverse oscillation manner,mirroring a regulatory scheme based on these two loci.Analysis of a panel of 2656 rice varieties suggests that the elite alleles of Ghd7 and ARE1 have undergone diversifying selection during breeding.Moreover,the allelic distribution of Ghd7 and ARE1 is associated with the soil nitrogen deposition rate in East Asia and South Asia.Remarkably,the combination of the Ghd7 and ARE1 elite alleles substantially improves NUE and yield performance under nitrogen-limiting conditions.Collectively,these results define a Ghd7–ARE1-based regulatory mechanism of nitrogen utilization,providing useful targets for genetic improvement of rice NUE. | Qing Wang Qingmei Su Jinqiang Nian Jian Zhang Meng Guo Guojun Dong Jiang Hu Rongsheng Wang Changshuo Wei Guanwen Li Wan Wang Hui-Shan Guo Shaoyang Lin Wenfeng Qian XianzhiXie Qian Qian Fan Chen Jianru Zuo | 2021 | Molecular Plant2021，14，6： | 10 |
| 14 | Numerical Study of Propulsion Mechanism for Oscillating Rigid and Flexible Tuna-Tails显示文摘 | Liang Yang Yumin Su Qing xiao | 2011 | Journal of Bionic Engineering2011，8，4： | 9 |
| 15 | WELL TESTING ANALYSIS FOR HORIZONTAL WELL WITH CONSIDERATION OF THRESHOLD PRESSURE GRADIENT IN TIGHT GAS RESERVOIRS显示文摘A fundamental solution for homogeneous reservoir in infinite space is derived by using the point source function with the consideration of the threshold pressure gradient. The fundamental solution of the continuous point source function is then derived based on the Green function. Various boundary conditions of the reservoirs are considered for this case and the corresponding solutions are obtained through the mirror image reflection and the principle of superimposition. The line source solution is obtained by integration. Subsequently, the horizontal-well bottom hole pressure response function for a non-linear gas flow in the homogeneous gas reservoir is obtained, and the response curve of the dimensionless bottom hole pressure and the derivative for a horizontal well in the homogeneous gas reservoir are obtained. In the end, the sensitivities of the relevant parameters are analyzed. The well test model presented in this paper can be used as the basis of the horizontal well test analysis for tight gas reservoirs. | GUO Jing-jing ZHANG Su ZHANG Lie-hui QING Hairuo LIU Qi-guo | 2012 | Journal of Hydrodynamics2012，24，4： | 9 |
| 16 | Relationship between Maternal PBMC HBV cccDNA and HBV Serological Markers and its Effect on HBV Intrauterine Transmission显示文摘Objective To investigate the relationship between maternal peripheral blood mononuclear cells (PBMC) hepatitis B virus(HBV) covalenty closed circular deoxyribonucleic acid(cccDNA) and other HBV serological markers and its effects on HBV intrauterine transmission. Methods We enrolled 290 newborns and their hepatitis B surface antigen(HBsAg) positive mothers. HBV cccDNA in PBMC and HBV DNA in serum were detected by a real‐time PCR‐TaqM an probe while HBV serological markers were detected with an electrochemiluminescence immunoassay. Results There was a positive correlation between the levels of PBMC HBV cccD NA and serum HBV DNA and HBeA g(r = 0.436 and 0.403, P < 0.001). The detection rate of pattern A [‘HBsA g(+), HBeA g(+), and anti‐HBc(+)’] was significantly higher in the PBMC HBV cccD NA positive group than in the control group(χ^2 = 48.48, P < 0.001). There was a significant association between HBV intrauterine transmission and PBMC HBV cccD NA(χ^2 = 9.28, P = 0.002). In the presence of serum HBV DNA, HBeA g, and PBMC HBV cccD NA, the risk of HBV intrauterine transmission was three times higher(OR = 3.69, 95% CI: 1.30‐10.42) than that observed in their absence. The risk of HBV intrauterine transmission was the greatest(OR = 5.89, 95% CI: 2.35‐14.72) when both PBMC HBV cccD NA and pattern A were present. A Bayesian network model showed that maternal PBMC HBV cccD NA was directly related to HBV intrauterine transmission. Conclusion PBMC HBV cccDNA may be a direct risk factor for HBV intrauterine transmission. Our study suggests that serological markers could be combined with PBMC‐related markers in prenatal testing. | WANG Dan Dan YI Lin Zhu WU Li Na YANG Zhi Qing HAO Hai Yun SHI Xiao Hong WANG Bo FENG Shu Ying FENG Yong Liang WANG Su Ping | 2019 | Biomedical and Environmental Sciences2019，32，5： | 8 |
| 17 | Malnutrition and Quality of Life in Chinese Cancer Patients: a Clinical Study of 23,994 Subjects显示文摘Background Malnutrition is common in patients with cancer,and can negatively impact their quality of life(QoL)and even survival.However,there is currently no large data available on the prevalence of malnutrition in Chinese cancer patients.This study evaluated the prevalence of malnutrition and the QoL of Chinese patients with locoregional,recurrent or metastatic cancer.Methods We conducted a nationwide observational,multi-center,hospital-based cross-sectional study within the Chinese Society of Nutritional Oncology(CSNO)Network.All of the patients were diagnosed with one of the following 18 different types of malignant tumors:lung cancer,gastric cancer,liver cancer,colorectal cancer,breast cancer,esophageal cancer,cervical cancer,endometrial cancer,nasopharyngeal carcinoma,malignant lymphoma,leukemia,pancreatic cancer,ovarian cancer,prostate cancer,bladder cancer,brain cancer,biliary tract malignant tumors or gastrointestinal stromal tumors.These patients were enrolled from 72 hospitals located in different regions of China.The patients’nutritional status was evaluated based on the body mass index(BMI),loss of bodyweight,laboratory measurements and patient generated-subjective global assessment(PG-SGA)scores.The cancer patients’physical status and QoL were assessed by the Karnofsky Performance Status(KPS)questionnaire and the European Organization for Research and Treatment of Cancer(EORTC)QLQ-C30 questionnaire,respectively.Results From December 2013 to April 2016,23,994 patients hospitalized for cancer treatment(such as surgery,chemotherapy or radiotherapy)were enrolled in the study.The patients included 12,494(52.9%)males and 11,124(47.1%)females.The mean age was 55.8±13.7 years.The proportions of patients in cancer stagesⅠ,Ⅱ,Ⅲ,Ⅳand uncertain were 11.5%,20.3%,27.5%,30.2%and 10.5%,respectively.Among the 23,994 inpatients,the proportions of patients who were underweight(BMI<18.5 kg/m2),normal(18.5 kg/m2 | Hong Xia Xu Chun Hua Song Zhen Ming Fu Chang Wang Zeng Qing Guo Yuan Lin Ying Ying Shi Wen Hu Yi Ba Su Yi Li Zeng Ning Li Kun Hua Wang Jing Wu Ying He Jia Jun Yang Cong Hua Xie Fu Xiang Zhou Xin Xia Song Gong Yan Chen Wen Jun Ma Su Xia Luo Zi Hua Chen Ming Hua Cong Hu Ma Chun Ling Zhou Wei Wang Qi Luo Yong Mei Shi Yu Mei Qi Hai Ping Jiang Wen Xian Guan Jun Qiang Chen Jia Xin Chen Yu Fang Lan Zhou Yong Dong Feng Rong Shao Tan Tao Li Jun Wen Ou Qing Chuan Zhao Jian Xiong Wu Min Weng Qing Hua Yao Jiu Wei Cui Wei Li Han Ping Shi the Investigation on Nutrition Status and Clinical Outcome of Common Cancers(INSCOC)Group | 2021 | Journal of Nutritional Oncology2021，6，1： | 8 |
| 18 | Screening of Serum Biomarkers for Distinguishing between Latent and Active Tuberculosis Using Proteome Microarray显示文摘Objective To identify potential serum biomarkers for distinguishing between latent tuberculosis infection(LTBI) and active tuberculosis(TB). Methods A proteome microarray containing 4,262 antigens was used for screening serum biomarkers of 40 serum samples from patients with LTBI and active TB at the systems level. The interaction network and functional classification of differentially expressed antigens were analyzed using STRING 10.0 and the TB database, respectively. Enzyme-linked immunosorbent assays(ELISA) were used to validate candidate antigens further using 279 samples. The diagnostic performances of candidate antigens were evaluated by receiver operating characteristic curve(ROC) analysis. Both antigen combination and logistic regression analysis were used to improve diagnostic ability. Results Microarray results showed that levels of 152 Mycobacterium tuberculosis(Mtb)-antigenspecific IgG were significantly higher in active TB patients than in LTBI patients(P < 0.05), and these differentially expressed antigens showed stronger associations with each other and were involved in various biological processes. Eleven candidate antigens were further validated using ELISA and showed consistent results in microarray analysis. ROC analysis showed that antigens Rv2031 c, Rv1408, and Rv2421 c had higher areas under the curve(AUCs) of 0.8520, 0.8152, and 0.7970, respectively. In addition, both antigen combination and logistic regression analysis improved the diagnostic ability. Conclusion Several antigens have the potential to serve as serum biomarkers for discrimination between LTBI and active TB. | CAO Shu Hui CHEN Yan Qing SUN Yong LIU Yang ZHENG Su Hua ZHANG Zhi Guo LI Chuan You | 2018 | Biomedical and Environmental Sciences2018，31，7： | 8 |
| 19 | Mechanism of exogenous nucleic acids and their precursors improving the repair of intestinal epithelium after 7-irradiation in mice显示文摘AIM To clone expressed genes associated withrepair of irradiation-damaged mice intestinalgland cells treated by small intestinal RNA,andto explore the molecular mechanism ofexogenous nucleic acids improving repair ofintestinal crypt.METHODS The animal mode of test group andcontrol group was established,forty-five micebeing irradiated by γ ray were treated with smallintestinal RNA as test group,forty mice beingirradiated by γ ray were treated withphysiological saline as control group,five micewithout irradiation were used as normal control,their jejunal specimens were collectedrespectively at 6h,12h,24h,4d and 8d afterirradiation.Then by using LD-PCR based onsubtractive hybridization,these gene fragmentsdifferentially expressed between test group andcontrol group were obtained,and then werecloned into T vectors as well as beingsequenced.Obtained sequences were screenedagainst.GeneBank,if being new sequences,they were submitted to GeneBank.RESULTS Ninety clones were associated withrepair of irradiation-damaged intestinal glandcells treated by intestinal RNA.These clonesfrom test group of 6h,12h,24h,4d and 8dwere respectively 18,22,25,13,12.By screening against GeneBank,18 of which werenew sequences,the others were dramaticallysimilar to the known sequences,mainly similarto hsp,Nmi,Dutt1,alkaline phosphatase,homeobox,anti-CEA ScFv antibody,arginine/serine kinase and BMP-4,repA.Eighteen genefragments were new sequences,their acceptnumbers in GeneBank were respectivelyAF240164-AF240181.CONCLUSION Ninety clones were obtained tobe associated with repair of irradiation-damagedmice intestinal gland cells treated by smallintestinal RNA,which may be related toabnormal expression of genes and matchedproteins of hsp,Nmi,Duttl,Na,K-ATPase,alkalineph-osphatase,glkA,single strandedreplicative centromeric gene as well as 18 newsequences. | Da Xiang Cui~1 Guei Ying Zeng~2 Feng Wang~1 Jun Rong Xu~1 Dong Qing Ren~2 Yan Hai Guo~1 Fu Rong Tian~2 Xiao Jun Yan~1 Yu Hou~1 Cheng Zhi Su~1 1 Institute of Genetic Diagnosis of the Fourth Military Medical University,Xi’an 710032,China 2 Department of Irradiation Medicine of the Fourth Military Medical University,Xi’an 710032,China | 2000 | World Journal of Gastroenterology2000，6，5： | 8 |
| 20 | 蓝莓花青素对RAW264.7细胞的抗炎活性研究及其机理初探(英文)显示文摘目的:以园蓝为研究材料,鉴定园蓝花青素提取物(GBBAEs)中的功能性成分的结构,建立脂多糖(LPS)诱导的体外炎症模型,并评价其抗炎作用和初步机制。创新点:首次探究了蓝莓花青素对建立的LPS诱导体外炎症模型的营养干预作用,并初步探究了发挥抗炎机制的作用通路。方法:将RAW 264.7细胞分为对照组(不作处理)和实验组(1μg/ml LPS刺激建模)。实验组进一步分为3个不同浓度组:400μg/ml GBBAEs组、800μg/ml GBBAEs组和1200μg/ml GBBAEs组。用酶联免疫吸附测定(ELISA)试剂盒检测一氧化氮(NO)、前列腺素E2(PGE2)、白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、干扰素γ(INF-γ)等炎症因子的释放量;用实时定量聚合酶链反应(RT-PCR)分析IL-1β、IL-6、TNF-α、环氧合酶-2(COX-2)及单核细胞趋化蛋白-1(MCP-1)的炎症相关基因m RNA的表达水平;用蛋白质印迹法(Western blot法)测定相关炎症蛋白COX-2和NF-κBp65表达水平。结论:试验结果表明,通过ELISA法测定GBBAEs可以显著性抑制NO、PGE2、IL-1β、IL-6、INF-γ等炎症因子的释放;RT-PCR分析阐明在LPS诱导的单核-巨噬细胞RAW 264.7中,GBBAEs可以显著性抑制IL-1β、IL-6、TNF-α、COX-2及MCP-1的炎症相关基因m RNA的表达水平。此外,Western blot法进一步显示GBBAEs对相关炎症蛋白COX-2和NF-κBp65表达具有明显抑制作用,进一步证实GBBAEs通过NF-κB机制通路来发挥抗炎作用。 | Wei XU Qing ZHOU Yong YAO Xing LI Jiu-liang ZHANG Guan-hua SU Ai-ping DENG | 2016 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2016，17，6： | 7 |
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