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| 1 | A Chromosome-Level Genome Assembly of Garlic (Allium sativum) Provides Insights into Genome Evolution and Allicin Biosynthesis显示文摘Garlic,an economically important vegetable,spice,and medicinal crop,produces highly enlarged bulbs and unique organosulfur compounds.Here,we report a chromosome-level genome assembly for garlic,with a total size of approximately 16.24 Gb,as well as the annotation of 57561 predicted protein-coding genes,making garlic the first Allium species with a sequenced genome.Analysis of this garlic genome assembly reveals a recent burst of transposable elements,explaining the substantial expansion of the garlic genome.We examined the evolution of certain genes associated with the biosynthesis of allicin and inulin neoseries-type fructans,and provided new insights into the biosynthesis of these two compounds.Furthermore,a large-scale transcriptome was produced to characterize the expression patterns of garlic genes in different tissues and at various growth stages of enlarged bulbs.The reference genome and large-scale transcriptome data generated in this study provide valuable new resources for research on garlic biology and breeding. | Xiudong Sun Siyuan Zhu Ningyang Li Yi Cheng Jing Zhao Xuguang Qiao Li Lu Shiqi Liu Yanzhou Wang Chan Liu Benping Li Wu Guo Shuang Gao Zemao Yang Fu Li Zheng Zeng Qing Tang Yupeng Pan Mengjiao Guan Jian Zhao Xiaomi ng Lu Huanwe n Meng Zhenlin Han Chun she ng Gao Wenkai Jiang Xing Zhao Shilin Tian Jianguang Su Zhihui Cheng Touming Liu | 2020 | Molecular Plant2020，13，9： | 14 |
| 2 | Adapting rice anther culture to gene transformation and RNA interference显示文摘Anther culture offers a rapid method of generating homozygous lines for breeding pro- gram and genetic analysis.To produce homozygous transgenic lines of rice(Oryza sativa L.)in one step,we developed an efficient protocol of anther-callus-based transformation mediated by Agro- bacterium after optimizing several factors influencing efficient transformation,including callus induc- tion and Agrobacterium density for co-cultivation.Using this protocol,we obtained 145 independent green transformants from five cultivars of japonica rice by transformation with a binary vector pCXK1301 bearing the rice gene,Xa21 for resistance to bacterial blight,of which 140 were further confirmed by PCR and Southern hybridization analysis,including haploids(32.1%),diploids(62.1%) and mixoploids(7.5%).Fifteen diploids were found to be doubled haploids,which accounted for 10.7%of the total positive lines.Finally,by including 28 from colchicine induced or spontaneous dip- loidization of haploids later after transformation,a total of 43 doubled haploids(30.7%)of Xa21 transgenic lines were obtained.We also generated two RNAi transgenic haploids of the rice Os- MADS2 gene,a putative redundant gene of OsMADS4 based on their sequence similarity,to inves- tigate its possible roles in rice flower development by this method.Flowers from the two OsMADS2 RNAi transgenic haploids displayed obvious homeotic alternations,in which lodicules were trans- formed into palea/lemma-like tissues,whereas identities of other floral organs were maintained.The phenotypic alternations were proved to result from specific transcriptional suppression of OsMADS2 gene by the introduced RNAi transgene.The results confirmed that OsMADS2 is involved in lodicule development of rice flower and functionally redundant with OsMADS4 gene.Our results demonstrated that rice anther culture could be adapted to gene transformation and RNAi analysis in rice. | CHEN Caiyan 1,2* ,XIAO Han 1,2* ,ZHANG Wenli 1,2 ,WANG Aiju 1,2 ,XIA Zhihui 1 ,LI Xiaobing 1 , ZHAI Wenxue 1 ,CHENG Zhukuan 1 &ZHU Lihuang 1 1.State Key Laboratory of Plant Genomics and National Center for Plant Gene Research,Institute of Genetics and Develop- mental Biology,Chinese Academy of Sciences,Beijing 100101,China 2.Graduate School of the Chinese Academy of Sciences,Beijing 100039,China | 2006 | Science China(Life Sciences)2006，49，5： | 5 |
| 3 | Patient-derived non-small cell lung cancer xenograft mirrors complex tumor heterogeneity显示文摘Objective:Patient-derived xenograft(PDX)models have shown great promise in preclinical and translational applications,but their consistency with primary tumors in phenotypic,genetic,and pharmacodynamic heterogeneity has not been well-studied.This study aimed to establish a PDX repository for non-small cell lung cancer(NSCLC)and to further elucidate whether it could preserve the heterogeneity within and between tumors in patients.Methods:A total of 75 surgically resected NSCLC specimens were implanted into immunodeficient NOD/SCID mice.Based on the successful establishment of the NSCLC PDX model,we compared the expressions of vimentin,Ki67,EGFR,and PD-L1 proteins between cancer tissues and PDX models using hematoxylin and eosin staining and immunohistochemical staining.In addition,we detected whole gene expression profiling between primary tumors and PDX generations.We also performed whole exome sequencing(WES)analysis in 17 first generation xenografts to further assess whether PDXs retained the patient heterogeneities.Finally,paclitaxel,cisplatin,doxorubicin,atezolizumab,afatininb,and AZD4547 were used to evaluate the responses of PDX models to the standard-of-care agents.Results:A large collection of serially transplantable PDX models for NSCLC were successfully developed.The histology and pathological immunohistochemistry of PDX xenografts were consistent with the patients’tumor samples.WES and RNA-seq further confirmed that PDX accurately replicated the molecular heterogeneities of primary tumors.Similar to clinical patients,PDX models responded differentially to the standard-of-care treatment,including chemo-,targeted-and immuno-therapeutics.Conclusions:Our established PDX models of NSCLC faithfully reproduced the molecular,histopathological,and therapeutic characteristics,as well as the corresponding tumor heterogeneities,which provides a clinically relevant platform for drug screening,biomarker discovery,and translational research. | Xuanming Chen Cheng Shen Zhe Wei Rui Zhang Yongsheng Wang Lili Jiang Ke Chen Shuang Qiu Yuanli Zhang Ting Zhang Bin Chen Yanjun Xu Qiyi Feng Jinxing Huang Zhihui Zhong Hongxia Li Guowei Che Kai Xiao | 2021 | Cancer Biology & Medicine2021，18，1： | 5 |
| 4 | Ectopic expression of VRT-A2 underlies the origin of Triticum polonicum and Triticum petropavlovskyi with long outer glumes and grains显示文摘Polish wheat (Triticum polonicum) is a unique tetraploid wheat species characterized by an elongated outer glume. The genetic control of the long-glume trait by a single semi-dominant locus, P1 (from Polish wheat), was established more than 100 years ago, but the underlying causal gene and molecular nature remain elusive. Here, we report the isolation of VRT-A2, encoding an SVP-clade MADS-box transcription factor, as the P1 candidate gene. Genetic evidence suggests that in T. polonicum, a naturally occurring sequence rearrangement in the intron-1 region of VRT-A2 leads to ectopic expression of VRT-A2 in floral organs where the long-glume phenotype appears. Interestingly, we found that the intron-1 region is a key ON/OFF molecular switch for VRT-A2 expression, not only because it recruits transcriptional repressors, but also because it confers intron-mediated transcriptional enhancement. Genotypic analyses using wheat accessions indicated that the P1 locus is likely derived from a single natural mutation in tetraploid wheat, which was subsequently inherited by hexaploid T. petropavlovskyi. Taken together, our findings highlight the promoter-proximal intron variation as a molecular basis for phenotypic differentiation, and thus species formation in Triticum plants. | Jing Liu Zhaoyan Chen Zhihui Wang Zhaoheng Zhang Xiaoming Xie Zihao Wang Lingling Chai Long Song Xuejiao Cheng Man Feng Xiaobo Wang Yanhong Liu Zhaorong Hu Jiewen Xing Zhenqi Su Huiru Peng Mingming Xin Yingyin Yao Weilong Guo Qixin Sun Jie Liu Zhongfu Ni | 2021 | Molecular Plant2021，14，9： | 4 |
| 5 | Genome-wide identification of target genes repressed by the zinc finger transcription factor REST/NRSF in the HEK 293 cell line显示文摘Transcriptional 压抑象在建立房间类型的 transcriptional 激活一样重要基因表示的特定的模式。沉默 RE1 抄写因素(休息) ,也作为 neuronal 知道限制 silencing 因素(NRSF ) ,是镇压的一个 transcriptional 管理者在 non-neuronal 房间或在由到特定的 DNA 的绑定的神经祖先房间的一组 neuronal 区别基因定序(抑压者 element-1/neuron-restrictive silencer 元素, RE1/NRSE ) 。然而,在 neuronal 发展的 REST/NRSF 功能几乎广泛地没被学习在各种各样的 non-neuronal 系对目标基因被知道可以导致房间区别。这里,我们使用技术与 microarray 策略相结合在人的 non-neuronal 房间线 HEK 293 识别潜在的 REST/NRSF 目标和 RE1/NRSEs 的 RNA 干扰(RNAi ) 。54 基因的表示根据 microarray 实验由在 HEK 293 房间的 REST/NRSF 的抑制是起来调整的, 13 那些被量的 RT-PCR 进一步证实。我们的结果证实电流的好信心和可靠性研究数据基于在 silico,在有 microarrays (薄片薄片) 的联合的染色质 immunoprecipitation,并且高产量的定序 ChIP-seq ) 。鉴于几千基因被证明了或预言了被 REST/NRSF 认识到的事实,然而,我们仅仅在那些之中的一些基因由和在基因的 RE1/NRSEs 地点的 REST/NRSF 的相互作用是直接起来调整的数据表演定序。 | Zhihui Liu Ming Liu Gang Niu Yi Cheng Jian Fei | 2009 | Acta Biochimica et Biophysica Sinica2009，41，12： | 3 |
| 6 | Synergetic Improvement of Interlaminar Fracture Toughness in Carbon Fiber/Epoxy Composites Interleaved with PES/PEK‑C Hybrid Nanofiber Veils显示文摘In this study,two types of soluble thermoplastic resins were added to epoxy resin at a fixed weight ratio to prepare a three-phase cast body.The cast was then manufactured into hybrid nanofiber as interleaves for interlaminar toughening of carbon fiber/epoxy resin(CF/EP)composites using a co-solvent method.The results revealed that when the hybrid components reached 15 wt%,Polyethersulfone(PES)and polyaryletherketone cardo(PEK-C)exhibited the best synergistic toughening effect,and the fracture toughness increased by 99.8%and 39.8%,respectively,compared with the reference or the same proportion of the single PES toughened sample.We used PES/PEK-C hybrid nanofibers with an areal density of 19.2 g per square meter(gsm)as composite toughening layers.Apart from the lack of significant influence of PES nanofiber on CF/EP composites,the interlaminar fracture toughness of mode I and mode II layers increased by 88.3%and 46.9%,respectively,compared to the reference sample.Scanning Electron Microscopy of the fracture surface and cross-section micromorphology of the laminate displayed that the thermoplastic microspheres of different sizes contribute differently to crack resistance:PEK-C consumes more energy due to the debonding and extraction of microspheres and resin,whereas the presence of the PES phase can induce more plastic deformation and crack deflection. | Jinli Zhou Chenyu Zhang Chao Cheng Ming Wang Zhihui Yang Yanfei Yang Hongying Yang Muhuo Yu | 2022 | Advanced Fiber Materials2022，4，5： | 3 |
| 7 | SSR markers development and their application in genetic diversity evaluation of garlic(Allium sativum)germplasm显示文摘Garlic(Allium sativum),an asexually propagated vegetable and medicinal crop,has abundant genetic variation.Genetic diversity evaluation based on molecular markers has apparent advantages since their genomic abundance,environment insensitivity,and non-tissue specific features.However,the limited number of available DNA markers,especially SSR markers,are insufficient to conduct related genetic diversity assessment studies in garlic.In this study,4372 EST-SSR markers were newly developed,and 12 polymorphic markers together with other 17 garlic SSR markers were used to assess the genetic diversity and population structure of 127 garlic accessions.The averaged polymorphism information content(PIC)of these 29 SSR markers was 0.36,ranging from 0.22 to 0.49.Seventy-nine polymorphic loci were detected among these accessions,with an average of 3.48 polymorphic loci per SSR.Both the clustering analyses based on either the genotype data of SSR markers or the phenotypic data of morphological traits obtained genetic distance divided the 127 garlic accessions into three clusters.Moreover,the Mantel test showed that genetic distance had no significant correlations with geographic distance,and weak correlations were found between genetic distance and the phenotypic traits.AMOVA analysis showed that the main genetic variation of this garlic germplasm collection existed in the within-population or cluster.Results of this study will be of great value for the genetic/breeding studies in garlic and enhance the utilization of these garlic germplasms. | Xiaxia Li Lijun Qiao Birong Chen Yujie Zheng Chengchen Zhi Siyu Zhang Yupeng Pan Zhihui Cheng | 2022 | Plant Diversity2022，44，5： | 2 |
| 8 | Enhanced perovskite electronic properties via A-site cation engineering显示文摘Organic-inorganic halide perovskites have emerged as excellent candidates for low-cost photovoltaics and optoelectronics.While the predominant recent trend in designing perovskites for efficient and stable solar cells has been to mix different A-site cations,the role of A-site cations is still limited to tune the lattice and bandgap of perovskites.Herein we compare the optoelectronic properties of acetamidinum(Ace)and guanidinium(Gua)mixed methylammonium lead iodide perovskites and shed a light on the hidden role of A-site cation on the carrier mobility of mixed-cation lead iodide perovskites.The cations do not affect the bandgap of the perovskites since the orbitals from Ace and Gua do not contribute to the band edges of the material.However,the mobility of the Ace mixed perovskite is significantly enhanced to be an order of magnitude higher than that of the pristine perovskite.We apply the Ace mixed perovskite in hole-conductor-free printable mesoscopic perovskite solar cells and obtain a stabilized PCE of over 18%(certified 17.7%),which is the highest certified efficiency so far. | Xufeng Xiao Yanmeng Chu Chunyu Zhang Zhihui Zhang Zexiong Qiu Cheng Qiu Huaixing Wang Anyi Mei Yaoguang Rong Gengzhao Xu Yue Hu Hongwei Han | 2021 | Fundamental Research2021，1，4： | 2 |
| 9 | SRAP markersfor fruit shape in cucumber显示文摘 | Huanwen Meng Shuxia Chen Zhihui Cheng | 2012 | Pakistan Journal of Botany2012，44，4： | 1 |
| 10 | Pyrene-tiaraed pillar[5]arene:Strong intramolecular excimer emission applicable for photo-writing显示文摘A pyrene-tiaraed pillar[5]a re ne derivative was synthesized,which showed a concentration-independent intensive excimer emission.Photolysis of the pyrene-tiaraed pillar[5]arene led to a switch from excimer to monomer emission,applicable to photo-writing. | Chao Peng Wenting Liang Jiecheng Ji Chunying Fan Kuppusamy Kanagaraj Wanhua Wu Guo Cheng Dan Su Zhihui Zhong Cheng Yang | 2021 | Chinese Chemical Letters2021，32，1： | 1 |
| 11 | A novel coumarin-based fluorescent probe for selective detection of bissulfite anions in water and sugar samples显示文摘 | Yutao Yang Fangjun Huo Jingjing Zhang Zhihui Xie Jianbin Chao Caixia Yin Hongbo Tong Diansheng Liu Shuo Jin Fangqin Cheng Xuxiu Yan | 2012 | Sensors & Actuators: B Chemical2012，，： | 1 |
| 12 | Efficient removal of Cr (Ⅵ) from aqueous solution with Fe@Fe2O3 core-shell nanowires显示文摘 | Ai Zhihui Cheng Ying Zhang Lizhi | 2008 | Environmcntal Science and Technology2008，42，18： | 1 |
| 13 | A New Data Hiding Scheme Based on DNA Sequence 显示文摘 | Guo Cheng Chang Chin Chen Wang Zhihui | 2012 | International Journal of Innovative Computing Infor- mation and Control2012，8，1： | 1 |
| 14 | Severe intra-abdominal bleeding leading to hemorrhagic shock, rectal perforation, and bilateral hydrothorax after stapling procedure for prolapsed hemorrhoids (PPH): is the transanal drainage feasible in this situation?显示文摘 | Qinghua Wang Jinlin Du Cheng Cai Jianping Wang Zhihui Dai | 2014 | International Journal of Colorectal Disease2014，，4： | 1 |
| 15 | Ferroelectric-gated ReS_(2)field-effect transistors for nonvolatile memory显示文摘Ferroelectric field-effect transistors(FeFET)with nondestructive readout capability have emerged as an attractive candidate for next-generation nonvolatile memory technology.Herein,we demonstrate ferroelectric-gated nonvolatile memory featuring a top gate architecture by combining multi-layer ReS_(2)with ferroelectric poly(vinylidene fluoride-trifluoroethylene)(P(VDF-TrFE))copolymer films.The ReS_(2)FeFET using hBN as substrate shows a large memory window of~30 V.Repeated write/erase operations are successfully performed by applying pulse voltage of±25 V with 1 ms width to the ferroelectric P(VDF-TrFE),and an ultra-high write/erase ratio of~107 can be achieved.Furthermore,the ReS_(2)FeFET shows stable data retention capability of longer than 2,000 s and reliable endurance of greater than 2,000 cycles.These characteristics highlight that such ferroelectricgated nonvolatile memory has great potential in future non-volatile memory applications. | Li Liu Hao Wang Qilong Wu Kang Wu Yuan Tian Haitao Yang Cheng Min Shen Lihong Bao Zhihui Qin Hong-Jun Gao | 2022 | Nano Research2022，15，6： | 1 |
| 16 | Sapphirine-Bearing Pelitic Granulite from Ailaoshan Orogen,West Yunnan,China: Metamorphic Conditions and Tectonic Setting显示文摘To reveal the petrological characteristics, metamorphic evolution histon and tectonic setting of the pelitic granulites from Ailaoshan Orogen, Uest Yunnan, China, a comprehensive study in mineral chemistry, petrogeochemistry and geochronology studies is presented in this paper. Two metamorphic stages of the granulites can be established:(1) the peak metamorphism recorded by the mineral assemblage of garnet, kyanite, K-feidspar and rutile, and the initial retrograde metamorphism shown by the mineral assemblage of garnet, sillimanite, sapphirine, spinel, K-feldspar, plagioclase and biotite;(2) the superim-posed metamorphism recorded by the mineral assemblage of biotite, muscovite, plagioclase, quartz and ilmenite. Zircon LA-ICP-MS U-Pb dating indicates that the protolith of the granulite was deposited after 337 Ma. The initial retrograde metamorphism occurred at P-T conditions of 8.6-12 kbar at 850-920℃ estimated by mineral assemblages, the low pressure limit of kyanite stability and GBPQ geothermobarometer in Indosinian (about 235 Ma), and the late superimposed metamorphism occurred at the P-T condition of 3.5-3.9 kbar at 572-576℃ estimated by GBPQ geothermobarometer since 33Ma. The first stage was related to the amalgamation of the South China and Indochina blocks during the Triassic, and the second stage was possibly related with the large scale sinistral slip-shearing since the Oligocene. It is inferred that the upper continental crust was suhducted/underthrusted to the lower continental crust (deeper than 30 km) and underwent granulite-facies metamorphism and then quickly exhumed to the middle-upper crust (10-12 km) and initial retrograde metamorphism occurred due to the collision of the Indochina and South China blocks during Indosinian, which was followed by superimposition of the second stage of metamorphism since the Oligocene. | Cheng Wei Yuhao Zhao Luhua Zhu Xuexiang Qi Zhihui Cai Xufeng Liu Guangfei Ma | 2019 | Journal of Earth Science2019，30，3： | 1 |
| 17 | Strain-Insensitive Hierarchically Structured Stretchable Microstrip Antennas for Robust Wireless Communication显示文摘As the key component of wireless data transmission and powering,stretchable antennas play an indispensable role in flexible/stretchable electronics.However,they often suffer from frequency detuning upon mechanical deformations;thus,their applications are limited to wireless sensing with wireless transmission capabilities remaining elusive.Here,a hierarchically structured stretchable microstrip antenna with meshed patterns arranged in an arched shape showcases tunable resonance frequency upon deformations with improved overall stretchability.The almost unchanged resonance frequency during deformations enables robust on-body wireless communication and RF energy harvesting,whereas the rapid changing resonance frequency with deformations allows for wireless sensing.The proposed stretchable microstrip antenna was demonstrated to communicate wirelessly with a transmitter(input power of−3 dBm)efficiently(i.e.,the receiving power higher than−100 dBm over a distance of 100 m)on human bodies even upon 25%stretching.The flexibility in structural engineering combined with the coupled mechanical-electromagnetic simulations,provides a versatile engineering toolkit to design stretchable microstrip antennas and other potential wireless devices for stretchable electronics. | Jia Zhu Senhao Zhang Ning Yi Chaoyun Song Donghai Qiu Zhihui Hu Bowen Li Chenghao Xing Hongbo Yang Qing Wang Huanyu Cheng | 2021 | Nano-Micro Letters2021，13，8： | 1 |
| 18 | Effcient removal ofCr (Ⅵ) from aqueous solution with Fe2Fe2O3 core-shell nanowires显示文摘 | Zhihui Ai Ying Cheng Lizhi Zhang | 2008 | Environ Sci Technol2008，42，： | 1 |
| 19 | Vibration Control in Thin Slab Hot Strip Mills显示文摘 | Yan Xiaoqiang Sun Zhihui Cheng Wei | 2011 | Ironmaking and Steelrnaking2011，38，4： | 1 |
| 20 | Canine bone marrow mesenchymal stromal cells with lentiviral mHCN4 gene transfer create cardiac pacemakers显示文摘 | CHENG Jun ZHANG Zhihui WEI Lu | 2012 | Cytotherapy2012，14，5： | 1 |
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