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| 1 | QTG-Seq Accelerates QTL Fine Mapping through QTL Partitioning and Whole-Genome Sequencing of Bulked Segregant Samples显示文摘Deciphering the genetic mechanisms underlying agronomic traits is of great importance for crop improvement. Most of these traits are controlled by multiple quantitative trait loci (QTLs), and identifying the underlying genes by conventional QTL fine-mapping is time-consuming and labor-intensive. Here, we devised a new method, named quantitative trait gene sequencing (QTG-seq), to accelerate QTL fine-mapping. QTGseq combines QTL partitioning to convert a quantitative trait into a near-qualitative trait, sequencing of bulked segregant pools from a large segregating population, and the use of a robust new algorithm for identifying candidate genes. Using QTG-seq, we fine-mapped a plant-height QTL in maize (Zea mays L.), qPH7, to a 300-kb genomic interval and verified that a gene encoding an NF-YC transcription factor was the functional gene. Functional analysis suggested that qPH7-encoding protein might influence plant height by interacting with a CO-like protein and an AP2 domain-containing protein. Selection footprint ana卜 ysis indicated that qPH7 was subject to strong selection during maize improvement. In summary, QTG-seq provides an efficient method for QTL fine-mapping in the era of “big data'. | Hongwei Zhang Xi Wang Qingchun Pan Pei Li Yunjun Liu Xiaoduo Lu Wanshun Zhong Minqi Li Linqian Han Juan Li Pingxi Wang Dongdong Li Yan Liu Qing Li Fang Yang Yuan-Ming Zhang Guoying Wang Lin Li | 2019 | Molecular Plant2019，12，3： | 13 |
| 2 | Different Fertilization Modes of Sugarcane in Latosolic Red Soil of Guangxi显示文摘[Objectives] This study aimed to compare the effects of water-fertilizer integrated drip irrigation modes and traditional fertilization mode on growth,yield,production costs and economic benefit of sugarcane in latosolic red soil of Guangxi. [Methods]A field test was conducted. [Results] The agronomic traits and comprehensive economic benefit of sugarcane under the water-fertilizer integrated drip irrigation modes were superior to those under the traditional fertilization mode. Among them,the comprehensive economic benefit of sugarcane with special granular fertilizer as the base fertilizer and water-fertilizer integrated drip irrigation as topdressing were the highest,increased by 15 813 kg/ha,an increase of 15. 8%. Considering the cost input,the comprehensive economic benefit increased by 5 676 yuan/ha. [Conclusions]Taking the production cost,economic benefit and input-output ratio into account,it is recommended to promote the application of water-fertilizer integrated drip irrigation mode in bases with water and fertilizer equipment conditions. | Shixie MENG Xiaomin WANG Guosheng HUANG Yanfei LU Pingxi HUANG Guoying LU | 2019 | Asian Agricultural Research2019，11，4： | 1 |
| 3 | AI based colorectal disease detection using real-time screening colonoscopy显示文摘Colonoscopy is an effective tool for early screening of colorectal diseases.However,the application of colonoscopy in distinguishing different intestinal diseases still faces great challenges of efficiency and accuracy.Here we constructed and evaluated a deep convolution neural network(CNN)model based on 117055 images from 16004 individuals,which achieved a high accuracy of 0.933 in the validation dataset in identifying patients with polyp,colitis,colorectal cancer(CRC)from normal.The proposed approach was further validated onmulti-center real-time colonoscopy videos and images,which achieved accurate diagnostic performance on detecting colorectal diseases with high accuracy and precision to generalize across external validation datasets.The diagnostic performance of the model was further compared to the skilled endoscopists and the novices.In addition,our model has potential in diagnosis of adenomatous polyp and hyperplastic polyp with an area under the receiver operating characteristic curve of 0.975.Our proposed CNN models have potential in assisting clinicians in making clinical decisions with efficiency during application. | Jiawei Jiang Qianrong Xie Zhuo Cheng Jianqiang Cai Tian Xia Hang Yang Bo Yang Hui Peng Xuesong Bai Mingque Yan Xue Li Jun Zhou Xuan Huang Liang Wang Haiyan Long Pingxi Wang Yanpeng Chu Fan-Wei Zeng Xiuqin Zhang Guangyu Wang Fanxin Zeng | 2021 | Precision Clinical Medicine2021，4，2： | 0 |
| 4 | Cloning of a new allele of ZmAMP1 and evaluation of its breeding value in hybrid maize显示文摘Gene resources associated with plant stature and flowering time are invaluable for maize breeding.In this study,using an F2:3population derived from a natural semi-dwarf mutant grmm and a normal inbred line Si 273,we identified a major pleiotropic QTL on the distal long arm of chromosome 1(qPH1_dla),and found that qPH1_dla controlled plant height,flowering time,ear and yield traits.qPH1_dla was finemapped to a 16 kb interval containing ZmAMP1,which was annotated as a glutamate carboxypeptidase.Allelism tests using two independent allelic mutants confirmed that ZmAMP1 was the causal gene.Realtime quantitative PCR and genomic sequence analysis suggested that a nonsynonymous mutation at the598th base of ZmAMP1 gene was the causal sequence variant for the dwarfism of grmm.This novel ZmAMP1 allele was named ZmAMP1_grmm.RNA sequencing using two pairs of near isogenic lines(NILs)showed that 84 up-regulated and 68 down-regulated genes in dwarf NILs were enriched in 15metabolic pathways.Finally,introgression of ZmAMP1_grmm into Zhengdan 958 and Xianyu 335 generated two improved F1lines.In field tests,they were semi-dwarf,early-flowering,lodging-resistant,and high-yielding under high-density planting conditions,suggesting that ZmAMP1_grmm is a promising Green Revolution gene for maize hybrid breeding. | Pingxi Wang Yuan Yang Dongdong Li Jialiang Xu Riliang Gu Jun Zheng Junjie Fu Jianhua Wang Hongwei Zhang | 2023 | The Crop Journal2023，11，1： | 0 |
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