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| 1 | Use of genotype-environment interactions to elucidate the pattern of maize root plasticity to nitrogen deficiency显示文摘Maize(Zea mays L.) root morphology exhibits a high degree of phenotypic plasticity to nitrogen(N) de ficiency,but the underlying genetic architecture remains to be investigated Using an advanced BC_4F_3 population,we investigated the root growth plasticity under two contrasted N levels and identi fied the quantitative trait loci(QTLs) with QTL-environment(Q×E)interaction effects. Principal components analysis(PCA) on changes of root traits to N de ficiency(D LN-HN) showed that root length and biomass contributed for 45.8% in the same magnitude and direction on the first PC,while root traits scattered highly on PC_2 and PC_3. Hierarchical cluster analysis on traits for D LN-HN further assigned the BC_4F_3 lines into six groups,in which the special phenotypic responses to N de ficiency was presented These results revealed the complicated root plasticity of maize in response to N de ficiency that can be caused by genotype environment(G×E) interactions. Furthermore,QTL mapping using a multi-environment analysis identi fied 35 QTLs for root traits. Nine of these QTLs exhibited signi ficant Q×E interaction effects. Taken together,our findings contribute to understanding the phenotypic and genotypic pattern of root plasticity to N de ficiency,which will be useful for developing maize tolerance cultivars to N de ficiency. | Pengcheng Li Zhongjuan Zhuang Hongguang Cai Shuai Cheng Ayaz Ali Soomro Zhigang Liu Riliang Gu Guohua Mi Lixing Yuan Fanjun Chen | 2016 | Journal of Integrative Plant Biology2016，58，3： | 8 |
| 2 | Enhancing phosphorus uptake efficiency through QTL-based selection for root system architecture in maize显示文摘Root system architecture(RSA) plays an important role in phosphorus(P) acquisition,but enhancing P use efficiency(PUE) in maize via genetic manipulation of RSA has not yet been reported.Here,using a maize recombinant inbred line(RIL) population,we investigated the genetic relationships between PUE and RSA,and developed P-efficient lines by selection of quantitative trait loci(QTLs) that coincide for both traits.In low-P(LP) fields,P uptake efficiency(Pup E) was more closely correlated with PUE(r =0.48-0.54),and RSA in hydroponics was significantly related to Pup E(r=0.25-0.30) but not to P utilization efficiency(Put E).QTL analysis detected a chromosome region where two QTLs for PUE,three for Pup E and three for RSA were assigned into two QTL clusters,Cl-bin3.04 a and Cl-bin3.04 b.These QTLs had favorable effects from alleles derived from the large-rooted and high-Pup E parent.Marker-assisted selection(MAS) identified nine advanced backcross-derived lines carrying Cl-bin3.04 a or Cl-bin3.04 b that displayed mean increases of 22%e26% in PUE in LP fields.Furthermore,a line L224 pyramiding Clbin3.04 a and Cl-bin3.04 b showed enhanced Pup E,relying mainly on changes in root morphology,rather than root physiology,under both hydroponic and field conditions.These results highlight the physiological and genetic contributions of RSA to maize Pup E,and provide a successful study case of developing P-efficient crops through QTL-based selection. | Riliang Gu Fanjun Chen Lizhi Long Hongguang Cai Zhigang Liu Jiabo Yang Lifeng Wang Huiyong Li Junhui Li Wenxin Liu Guohua Mi Fusuo Zhang Lixing Yuan | 2016 | Journal of Genetics and Genomics2016，43，11： | 3 |
| 3 | QTL analysis across multiple environments reveals promising chromosome regions associated with yield-related traits in maize under drought conditions显示文摘Drought is one of the most critical abiotic stresses influencing maize yield. Improving maize cultivars with drought tolerance using marker-assisted selection requires a better understanding of its genetic basis. In this study, a doubled haploid(DH) population consisting of 217 lines was created by crossing the inbred lines Han 21(drought-tolerant) and Ye 478(drought-sensitive). The population was genotyped with a 6 K SNP assay and 756 SNP(single nucleotide polymorphism) markers were used to construct a linkage map with a length of 1344 c M. Grain yield(GY), ear setting percentage(ESP), and anthesis–silking interval(ASI) were recorded in seven environments under well-watered(WW) and water-stressed(WS) regimes. High phenotypic variation was observed for all traits under both water regimes. Using the LSMEAN(least-squares mean) values from all environments for each trait, 18 QTL were detected, with 9 associated with the WW and 9 with the WS regime. Four chromosome regions,Chr. 3: 219.8–223.7 Mb, Chr. 5: 191.5–194.7 Mb, Chr. 7: 132.2–135.6 Mb, and Chr. 10: 88.2–89.4 Mb, harbored at least 2 QTL in each region, and QTL co-located in a region inherited favorable alleles from the same parent. A set of 64 drought-tolerant BC_3F_6 lines showed preferential accumulation of the favorable alleles in these four regions, supporting an association between the four regions and maize drought tolerance. QTL-by-environment interaction analysis revealed 28 ed QTL(environment-dependent QTL) associated with the WS regime and 22 associated with the WW regime for GY, ESP, and ASI. All WS QTL and55.6% of WW QTL were located in the ed QTL regions. The hotspot genomic regions identified in this work will support further fine mapping and marker-assisted breeding of drought-tolerant maize. | Xinmin Hu Guihua Wang Xuemei Du Hongwei Zhang Zhenxiang Xu Jie Wang Guo Chen Bo Wang Xuhui Li Xunji Chen Junjie Fu Jun Zheng Jianhua Wang Riliang Gu Guoying Wang | 2021 | The Crop Journal2021，9，4： | 3 |
| 4 | Comparative Expression and Phylogenetic Analysis of Maize Cytokinin Dehydrogenase/Oxidase (CKX) Gene Family显示文摘 | Riliang Gu Junjie Fu Song Guo Fengying Duan Zhangkui Wang Guohua Mi Lixing Yuan | 2010 | Journal of Plant Growth Regulation2010，，4： | 2 |
| 5 | A nonsymbiotic hemoglobin gene from maize, ZmHb, is involved in response to submergence, high-salt and osmotic stresses显示文摘 | Li Zhao Riliang Gu Peng Gao Guoying Wang | 2008 | Plant Cell Tissue and Organ Culture2008，，2： | 1 |
| 6 | A nonsymbiotic hemoglobin gene from maize, ZmHb, is involved in response to submergence,high-salt and osmotic stresses 显示文摘 | Li Zhao Riliang Gu Peng Gao | 2008 | Plant Cell Tiss Organ Cult2008，95，22： | 1 |
| 7 | Isolation and Analysis of Cold Stress Inducible Genes in Zea mays by Suppression Subtractive Hybridization and cDNA Macroarray显示文摘 | Ying Zhang Junjie Fu Riliang Gu Jianhua Wang Xiaoping Chen Jinping Jia Jinpeng Zhang Guoying Wang | 2009 | Plant Molecular Biology Reporter2009，，1： | 1 |
| 8 | Cloning of a new allele of ZmAMP1 and evaluation of its breeding value in hybrid maize显示文摘Gene resources associated with plant stature and flowering time are invaluable for maize breeding.In this study,using an F2:3population derived from a natural semi-dwarf mutant grmm and a normal inbred line Si 273,we identified a major pleiotropic QTL on the distal long arm of chromosome 1(qPH1_dla),and found that qPH1_dla controlled plant height,flowering time,ear and yield traits.qPH1_dla was finemapped to a 16 kb interval containing ZmAMP1,which was annotated as a glutamate carboxypeptidase.Allelism tests using two independent allelic mutants confirmed that ZmAMP1 was the causal gene.Realtime quantitative PCR and genomic sequence analysis suggested that a nonsynonymous mutation at the598th base of ZmAMP1 gene was the causal sequence variant for the dwarfism of grmm.This novel ZmAMP1 allele was named ZmAMP1_grmm.RNA sequencing using two pairs of near isogenic lines(NILs)showed that 84 up-regulated and 68 down-regulated genes in dwarf NILs were enriched in 15metabolic pathways.Finally,introgression of ZmAMP1_grmm into Zhengdan 958 and Xianyu 335 generated two improved F1lines.In field tests,they were semi-dwarf,early-flowering,lodging-resistant,and high-yielding under high-density planting conditions,suggesting that ZmAMP1_grmm is a promising Green Revolution gene for maize hybrid breeding. | Pingxi Wang Yuan Yang Dongdong Li Jialiang Xu Riliang Gu Jun Zheng Junjie Fu Jianhua Wang Hongwei Zhang | 2023 | The Crop Journal2023，11，1： | 0 |
| 9 | qSTA2-2,a novel QTL that contributes to seed starch synthesis in Zea mays L.显示文摘The seed storage materials accumulate during seed development,and are essential for seed germination and seedling establishment.Here we employed two bi-parental populations of an F2:3 population developed from a cross of improved 220(I220,small seeds with low starch)and PH4CV(large seeds with high starch),as well as recombinant-inbred lines(RILs)of X178(high starch)and its improved introgression line I178(low starch),to identify the genes that control seed storage materials.We identified a total of 12 QTLs for starch,protein and oil,which explained 3.44-10.79%of the phenotypic variances.Among them,qSTA2-1 identified in F2:3 and qSTA2-2 identified in the RILs partially overlapped at an interval of 7.314-9.554 Mb,and they explained 3.44-10.21%of the starch content variation,so they were selected for further study.Fine mapping of qSTA2-2 with the backcrossed populations of ^(I220)/PH4CV in each generation narrowed it down to a 199.7 kb interval that contains 14 open reading frames(ORFs).Transcriptomic analysis of developing seeds from the near-isogenic lines(NILs)of ^(I220)/PH4CV(BC_(5)F_(2))showed that only 11 ORFs were expressed in 20 days after pollination(DAP)seeds.Five of them were upregulated and six of them were downregulated in NIL^(I220),and the differentially expressed genes(DEGs)between NIL^(I220) and NIL^(PH4CV) were enriched in starch metabolism,hormone signal transduction and glycosaminoglycan degradation.Of the eleven NIL^(I220) differential expressed ORFs,ORF4(Zm00001d002260)and ORF5(Zm00001d002261)carry 75%protein sequence similarity,both encodes an glycolate oxidase,were the possible candidates of qSTA2-2.Further analysis and validation indicated that mutation of the qSTA2-2 locus resulted in the dysfunction of ABA accumulation,the embryo/endosperm ratio and the starch and hormone levels. | Minghao Cai Xuhui Li Zhi Liang Jie Wang Delin Li Zhipeng Yuan Riliang Gu Jianhua Wang Li Li | 2024 | Journal of Integrative Agriculture2024，23，4： | 0 |
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