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| 1 | Changes in growth factor and cytokine expression in biliary obstructed rat liver and their relationship with delayed liver regeneration after partial hepatectomy显示文摘瞄准:在部分肝切除术以后在肝新生上学习妨碍的黄疸的效果。方法: Hepatocyte 生长因素( HGF ),它的受体, 遇见c ,脉管的内皮生长因素( VEGF )和转变生长在肝织物和孤立的肝细胞的 factor-beta1 ( TGF-beta1 ) mRNA 表示被量的反向抄写的聚合酶链反应( RT-PCR )用 LightCycler 在胆汁的阻塞( BO )以后调查。为 desmin 和 alpha 光滑的肌肉肌动朊(alpha-SMA ) 染色的 Immunohistochemical 也被学习。标记的原子抗原(PCNA ) 索引的再生肝重量和增殖的房间,和生长因素表示然后与伴随物在 70% 肝切除术以后被评估在 BO 老鼠或假冒操作老鼠的内部胆汁的排水。结果:肝的 TGF-beta1 mRNA 层次显著地在 BO 以后增加了 14 天,并且进一步与胆汁郁积的持续时间增加了。同时, HGF 和 VEGF 趋于增加,但是不是重要的。在房间孤立, TGF-beta1 mRNA 主要在肝的星形的房间(HSC ) 被发现部分。Immunohistochemical 研究揭示了 HSC (desmin 积极的房间) 的一个增加的数字并且在 BO 以后在门区域激活 HSC (alpha-SMA-positive 房间) 。在一个肝切除术模型,肝新生在 BO 老鼠被推迟,作为与假冒操作老鼠相比。在肝切除术,和更早的 HGF mRNA 山峰沉醉于 BO 老鼠以后, TGF-beta1 mRNA 是显著地起来调整的直到 48 h。结论:BO 导致 HSC 增长和激活,导致 TGF-beta1 mRNA 的起来规定和在肝的 HGF mRNA 的抑制。这些改变的表示模式可以强烈与妨碍的黄疸在肝切除术以后涉及推迟的肝新生。 | Hironobu Makino Hiroaki Shimizu Hiroshi Ito Fumio Kimura Satoshi Ambiru Akira Togawa Masayuki Ohtsuka Hiroyuki Yoshidome Atsushi Kato Hideyuki Yoshitomi Shigeaki Sawada Masaru Miyazaki | 2006 | World Journal of Gastroenterology2006，12，13： | 32 |
| 2 | Diagnostic and therapeutic single-operator cholangiopancreatoscopy in biliopancreatic diseases:Prospective multicenter study in Japan显示文摘AIM: To assess the utility and safety of single-operator cholangiopancreatoscopy(SOCPS) using the Spy Glass system in widespread clinical application for biliary and pancreatic diseases.METHODS: This study was a prospective case series conducted in 20 referral centers in Japan. There were 148 patients who underwent SOCPS; 124 for biliary diseases and 24 for pancreatic diseases. The attempted interventions were SOCPS examination, SOCPS-directed tissue sampling, and therapy for stone removal, among others. The main outcomes were related to the procedure success rate in terms of visualizing the target lesions, SOCPS-directed adequate tissue sampling, and complete stone removal. RESULTS: A total of 148 patients were enrolled for the diagnosis of indeterminate biliary and pancreatic lesions or treatment of biliary and pancreatic disease. The overall procedure success rate of visualizing the target lesions was 91.2%(135/148). The overall procedural success rates of visualizing the target lesions of diagnostic SOCPS in the bile duct and pancreatic duct were 95.5%(84/89) and 88.2%(15/17), respectively. Diagnosis: the overall adequate tissue for histologic examination was secured in 81.4% of the 86 patients who underwent biopsy under SOCPS(bile duct, 60/75, 80.0%; pancreatic duct, 10/11, 90.9%). The accuracy of histologic diagnosis using SOCPS-directed biopsies in indeterminate bile duct lesions was 70.7%(53/75). In the pancreatic duct, the accuracy of SOCPS visual impression of intraductal papillary mucinous neoplasm was 87.5%(14/16). Stone therapy: complete biliary and pancreatic stone clearance combined with SOCPS-directed stone therapy using electrohydraulic lithotripsy or laser lithotripsy was achieved in 74.2%(23/31) and 42.9%(3/7) of the patients, respectively. Others: SOCPS using the Spy Glass system was used in cannulation of the cystic duct in two patients and for passing across the obstructed self-expandable metallic stent for a malignant biliary stricture in two patients. All procedures were successful in both SOCPS-guided therapies. The incidence of procedure-related adverse events was 5.4%(8/148). CONCLUSION: SOCPS with direct visualization and biopsy for diagnosis and SOCPS-directed therapy for biliary and pancreatic diseases can be safely performed with a high success rate. | Toshio Kurihara Ichiro Yasuda Hiroyuki Isayama Toshio Tsuyuguchi Taketo Yamaguchi Ken Kawabe Yoshinobu Okabe Keiji Hanada Tsuyoshi Hayashi Takao Ohtsuka Syuhei Oana Hiroshi Kawakami Yoshinori Igarashi Kazuya Matsumoto Kiichi Tamada Shomei Ryozawa Hiroki Kawashima Yutaka Okamoto Iruru Maetani Hiroyuki Inoue Takao Itoi | 2016 | World Journal of Gastroenterology2016，22，5： | 20 |
| 3 | Vascular endothelial growth factor and angiopoietins regulate sinusoidal regeneration and remodeling after partial hepatectomy in rats显示文摘AIM: To study the regulatory mechanisms of sinusoidal regeneration after partial hepatectomy.METHODS: We investigated the expression of angiopoietin (Ang)-1, Ang-2, Tie-2, and vascular endothelial growth factor (VEGF) in regenerating liver tissue by quantitative reverse-transcription polymerase chain reaction (RT-PCR) using a LightCycler (Roche Diagnostics) and also immunohistochemical staining after 70% hepatectomy in rats. In the next step, we isolated liver cells (hepatocytes,sinusoidal endothelial cell (SEC), Kupffer cell, and hepatic stellate cells (HSC)) from regenerating liver tissue by in situ collagenase perfusion and counterflow elutriation,to determine potential cellular sources of these angiogenic factors after hepatectomy. Proliferation and apoptosis of SECs were also evaluated by proliferating cell nuclear antigen (PCNA) staining and the terminal deoxynucleotidyl transferase d-uridine triphosphate nick end labeling (TUNEL) assay, respectively.RESULTS: VEGF mRNA expression increased with a peak at 72 h after hepatectomy, decreasing thereafter.The expression of Ang-1 mRNA was present at detectable levels before hepatectomy and increased slowly with a peak at 96 h. Meanwhile, Ang-2 mRNA was hardly detected before hepatectomy, but was remarkably induced at 120 and 144 h. In isolated cells, VEGF mRNA expression was found mainly in the hepatocyte fraction.Meanwhile, mRNA for Ang-1 and Ang-2 was foundin the SEC and HSC fractions, but was more prominent in the latter. The PCNA labeling index of SECs increased slowly,reaching a peak at 72 h, whereas apoptotic SECs were detected between 120 h and 144 h.CONCLUSION: Ang-Tie system, together with VEGF,plays a critical role in regulating balance between SEC proliferation and apoptosis during sinusoidal regeneration after hepatectomy. However, the VEGF system plays a more important role in the early phase of sinusoidal regeneration than angiopoietin/Tie system. | Hiroaki Shimizu Noboru Mitsuhashi Masayuki Ohtsuka Hiroshi Ito Fumio Kimura Satoshi Ambiru Akira Togawa Hiroyuki Yoshidome Atsushi Kato Masaru Miyazaki | 2005 | World Journal of Gastroenterology2005，11，46： | 17 |
| 4 | Effect of high magnetic field on phase transformation temperature and microstructure of Fe-based Alloys显示文摘The effect of a high magnetic field up to 30T on phase transformation temperature and microstructure of Fe-based alloys has been reviewed. A high magnetic field accelerates ferrite transformation, changes the morphology of the transformed microstructures and increases the A3 and A1 temperature. In a magnetic field of 30T, the A1 temperature increases by about 37.1℃ for Fe-0.8C, the A3 temperature for pure Fe increases by about 33.1℃. The measured transformation temperature data are not consistent with calculation results using Weiss molecular field theory. Ferrite grains are elongated and aligned along the direction of magnetic field in Fe-0.4C and Fe-0.6C alloys by ferrite transformation, but elongated and aligned structure was not found in pure Fe, Fe-0.05C alloy and Fe-1.5Mn-0.11C-0.1V alloy. | 郝新江 OHTSUKA Hideyuki | 2005 | 材料与冶金学报2005，4，2： | 13 |
| 5 | Peripheral portal vein-oriented non-dilated bile duct puncture for percutaneous transhepatic biliary drainage显示文摘AIM: To evaluate the efficacy of peripheral portal vein(PV)-oriented non-dilated bile duct(BD) puncture for percutaneous transhepatic biliary drainage(PTBD).METHODS: Thirty-five patients with non-dilated BDs underwent PTBD for the management of various biliary disorders, including benign bilioenteric anastomotic stricture(n = 24), BD stricture(n = 5) associated with iatrogenic BD injury, and postoperative biliary leakage(n = 6). Under ultrasonographic guidance, percutaneous transhepatic puncture using a 21-G needle was performed along the running course of the peripheral targeted non-dilated BD(preferably B6 for right-sided approach, and B3 for left-sided approach) or along the accompanying PV when the BD was not well visualized. This technique could provide an appropriate insertion angle of less than 30° between the puncture needle and BD running course. The puncture needle was then advanced slightly beyond the accompanying PV. The needle tip was moved slightly backward while injecting a small amount of contrast agent to obtain the BD image, followed by insertion of a 0.018-inch guide wire(GW). A drainage catheter was then placed usinga two-step GW method. RESULTS: PTBD was successful in 33(94.3%) of the 35 patients with non-dilated intrahepatic BDs. A rightsided approach was performed in 25 cases, while a left-sided approach was performed in 10 cases. In 31 patients, the first PTBD attempt proved successful. Four cases required a second attempt a few days later to place a drainage catheter. PTBD was successful in two cases, but the second attempt also failed in the other two cases, probably due to poor breath-holding ability. Although most patients(n = 26) had been experiencing cholangitis with fever(including septic condition in 8 cases) before PTBD, only 5(14.3%) patients encountered PTBD procedure-related complications, such as transient hemobilia and cholangitis. No major complications such as bilioarterial fistula or portal thrombosis were observed. There was no mortality in our series.CONCLUSION: Peripheral PV-oriented BD puncture for PTBD in patients with non-dilated BDs is a safe and effective procedure for BD stricture and postoperative bile leakage. | Hiroaki Shimizu Atsushi Kato Tsukasa Takayashiki Satoshi Kuboki Masayuki Ohtsuka Hideyuki Yoshitomi Katsunori Furukawa Masaru Miyazaki | 2015 | World Journal of Gastroenterology2015，21，44： | 10 |
| 6 | Patterns of plant diversity at high altitudes on the Qinghai-Tibetan Plateau显示文摘To describe the biodiversity patterns of plants along an altitudinal gradient on the Qinghai-Tibetan Plateau and to clarify the bias in plant specimen records at high altitude.Methods We conducted a large-scale investigation of vegetation at a wide range of altitudes,focusing on a high-altitudinal range(3200-5200 m)at different locations on the Qinghai-Tibetan Plateau.We then compared the altitudinal distribution of plant species obtained from our field investigation with that in plant specimen records from published sources and an online database.Important Findings Our data provide evidence that altitude plays a large role in regulating species composition on the Qinghai-Tibetan Plateau.We could not,however,detect a clear relationship between altitude and species richness,although a weak monotonically increasing trend of richness was detected with increasing altitude.According to specimen records,most species have been sampled at a wide range of altitudes,and the average range of 145 species is>2000 m.Despite this wide range,more than half of the species we observed were at higher altitudes than the specimen records indicate.High-altitude areas have probably been so poorly sampled that only a small fraction of the resident species has been recorded.This study clearly shows the regional bias of specimen records in the Qinghai-Tibetan Plateau. | Ayako Shimono Huakun Zhou Haihua Shen Mitsuru Hirota Toshiyuki Ohtsuka Yanhong Tang | 2010 | Journal of Plant Ecology2010，3，1： | 6 |
| 7 | Imaging pitfalls of pancreatic serous cystic neoplasm and its potential mimickers显示文摘The aim of this article is to clarify diagnostic pitfalls of pancreatic serous cystic neoplasm(SCN) that may result in erroneous characterization. Usual and unusual imaging findings of SCN as well as potential SCN mimickers are presented. The diagnostic key of SCN is to look for a cluster of microcysts(honeycomb pattern), which may not be always found in the center. Fibrosis in SCN may be mistaken for a mural nodule of intraductal papillary mucinous neoplasm(IPMN). The absence of cyst wall enhancement may be helpful to distinguish SCN from mucinous cystic neoplasm. However, oligocystic SCN and branch duct type IPMN may morphologically overlap. In addition, solid serous adenoma, an extremely rare variant of SCN, is difficult to distinguish from neuroendocrine tumor. | Kousei Ishigami Akihiro Nishie Yoshiki Asayama Yasuhiro Ushijima Yukihisa Takayama Nobuhiro Fujita Shunichi Takahata Takao Ohtsuka Tetsuhide Ito Hisato Igarashi Shuji Ikari Catherine M Metz Hiroshi Honda | 2014 | World Journal of Radiology2014，6，3： | 6 |
| 8 | Endocytoscopic narrow-band imaging efficiency for evaluation of inflammatory activity in ulcerative colitis显示文摘AIM:To assess the efficacy of endocytoscopic narrowband imaging(EC-NBI)for evaluating the severity of inflammation in ulcerative colitis(UC).METHODS:This retrospective study was conducted at a single tertiary care referral center.We included UC patients who underwent colonoscopy with endocytoscopy from July 2010 to December 2013.ECNBI was performed,and the images were evaluated by assessing visibility,increased vascularization,and the increased calibers of capillaries and were classified as Obscure,Visible or Dilated.Obscure was indicative of inactive disease,while Visible and Dilated were indicative of acute inflammation.This study received Institutional Review Board approval.The primary outcome measures included the diagnostic ability of EC-NBI to distinguish between active and inactive UC on the basis of histological activity.The conventional endoscopic images were classified according to the Mayo endoscopic score.A score of 0 or 1 indicated inactive disease,whereas a score of 2 indicated active disease.RESULTS:Fifty-two patients were enrolled.There was a strong correlation between the EC-NBI findings and the histological assessment(r=0.871,P<0.01).The sensitivity,specificity,positive predictive value,negative predictive value,and accuracy of EC-NBI for diagnosing acute inflammation were 84.0%,100%,87.1%,100%,and 92.3%,respectively,while those for the Mayo endoscopic score were 100%,40.7%,100%,61.0%,and 69.2%,respectively.Compared with conventional endoscopy,EC-NBI was superior in diagnostic specificity,negative predictive value,and accuracy(P<0.001,P=0.001 and P=0.047,respectively).CONCLUSION:The EC-NBI finding of capillaries in the rectal mucosa was strongly correlated with histological inflammation and aided in the differential diagnosis between active and inactive UC. | Yasuharu Maeda Kazuo Ohtsuka Shin-ei Kudo Kunihiko Wakamura Yuichi Mori Noriyuki Ogata Yoshiki Wada Masashi Misawa Akihiro Yamauchi Seiko Hayashi Toyoki Kudo Takemasa Hayashi Hideyuki Miyachi Fuyuhiko Yamamura Fumio Ishida Haruhiro Inoue Shigeharu Hamatani | 2015 | World Journal of Gastroenterology2015，21，7： | 4 |
| 9 | 一项对224例日本眼眶肿瘤患者21年的回顾:起源和部位 | Ohtsuka K. Hashimoto M. Suzuki Y. 韩静 | 2005 | 世界核心医学期刊文摘(眼科学分册)2005，0，6： | 3 |
| 10 | Clinicopathological features and prognosis of surgical resected cases of biliary cancer with pancreaticobiliary maljunction显示文摘To the Editor:Pancreaticobiliary maljunction(PBM)is a congenital anomaly in which the pancreatic and bile ducts join anatomically outside of the duodenal wall away from the Oddi’s sphincter.This condition causes the reflux of pancreatic juice into the bile duct under high pressure,resulting in various pathologic changes.The features of PBM patients are common bile duct dilatation,long common channel,and high amylase levels in bile juice.Among them,one of the most significant problems is the development of biliary cancer,including extrahepatic bile duct and gallbladder cancers[1].A nationwide survey in Japan reported biliary cancer in 21.6%of adult patients with PBM concomitant with congenital biliary dilatation,32.1%and 62.3%extrahepatic bile duct and gallbladder cancers,respectively[2]. | Tsukasa Takayashiki Hideyuki Yoshitomi Katsunori Furukawa Satoshi Kuboki Masaru Miyazaki Masayuki Ohtsuka | 2020 | Hepatobiliary & Pancreatic Diseases International2020，19，1： | 3 |
| 11 | Current status of diagnosis and therapy for intraductal papillary neoplasm of the bile duct显示文摘Bile duct epithelial tumours showing papillary neoplasm in the bile duct lumen are present in the intrahepatic and extrahepatic bile ducts.Clinicopathological images of these tumours are distinctive and diverse,including histological images with a low to high grade dysplasia,infiltrating and noninfiltrating characteristics,excessive mucus production,and similarity to intraductal papillary mucinous neoplasm(IPMN)of the pancreas.The World Health Organization Classification of Tumours of the Digestive System in 2010 named these features,intraductal papillary neoplasm of the bile duct(IPNB),as precancerous lesion of biliary carcinoma.IPNB is currently classified into type 1 that is similar to IPMN,and type 2 that is not similar to IPMN.Many of IPNB spreads superficially,and diagnosis with cholangioscopy is considered mandatory to identify accurate localization and progression.Prognosis of IPNB is said to be better than normal bile duct cancer. | Yuji Sakai Masayuki Ohtsuka Harutoshi Sugiyama Rintaro Mikata Shin Yasui Izumi Ohno Yotaro Iino Jun Kato Toshio Tsuyuguchi Naoya Kato | 2021 | World Journal of Gastroenterology2021，27，15： | 3 |
| 12 | Ultraviolet and visible upconversion luminescence of Tm(0.1)Yb(5):FOV oxyfluoride nanophase vitroceramics显示文摘The ultraviolet upconversion luminescence of Tm3+ ions sensitized by Yb3+ ions in oxyfluoride nanophase vitroceramics when excited by a 975 nm diode laser was studied. An ultraviolet upconversion luminescence line positioned at 363.6 nm was found. It was attributed to the fluorescence transition of 1D2→3H6 of Tm3+ ion. Sev- eral visible upconversion luminescence lines at 450.7 nm, (477.0 nm, 462.5 nm), 648.5 nm, (680.5 nm, 699.5 nm) and (777.2 nm, 800.7 nm) were also found, which result respectively from the fluorescence transitions of 1D2→3F4, 1G4→3H6, 1G4→3F4, 3F3→3H6 and 3H4→3H6 of Tm3+ ion. The careful measurement and analysis of the variation of upconversion luminescence intensity F as a function of the 975 nm pumping laser power P prove that the upconversion luminescence of 1D2 state is partly a five-photon upconversion luminescence, and the upconversion lumines- cence of 1G4 state and 3H4 state are respectively the three-photon and two-photon upconversion luminescence. The theoretical analysis suggested that the upcon- version mechanism of the 363.6 nm 1D2→3H6 upconversion luminescence is partly the cross energy transfer of {3H4(Tm3+)→3F4(Tm3+), 1G4(Tm3+)→1D2(Tm3+)} and {1G4(Tm3+)→3F4(Tm3+), 3H4(Tm3+)→1D2(Tm3+)} between Tm3+ ions. In addition, the upconversion luminescence of 1G4 and 3H4 state results respectively from the se- quential energy transfer {2F5/2(Yb3+)→2F7/2(Yb3+), 3H4(Tm3+)→1G4(Tm3+)} and {2F5/2(Yb3+) →2F7/2(Yb3+), 3F4(Tm3+)→3F2(Tm3+)} from Yb3+ ions to Tm3+ ions. | N. SAWANOBOI M. OHTSUKA | 2008 | Science China(Physics,Mechanics & Astronomy)2008，51，12： | 3 |
| 13 | A H optimal theory-based generator control system显示文摘 | Ohtsuka K Taniguchi T Sato T | 1992 | IEEE Transactions on Energy Conversion1992，7，1： | 2 |
| 14 | Intraductal Papillary Mucinous Neoplasms of the Pancreas With Distinct Pancreatic Ductal Adenocarcinomas Are Frequently of Gastric Subtype显示文摘 | Noboru Ideno Takao Ohtsuka Hiroshi Kono Kenji Fujiwara Yasunori Oda Shinichi Aishima Tetsuhide Ito Kousei Ishigami Shoji Tokunaga Kenoki Ohuchida Shunichi Takahata Masafumi Nakamura Kazuhiro Mizumoto Masao Tanaka | 2013 | Annals of Surgery2013，，1： | 2 |
| 15 | Octreotide prevents l -asparaginase–induced pancreatic injury in rats显示文摘 | Mitsuyoshi Suzuki Toshiaki Shimizu Takahiro Kudo Hiromichi Shoji Yoshikazu Ohtsuka Yuichiro Yamashiro | 2008 | Experimental Hematology2008，，2： | 2 |
| 16 | The role of shrub(Potentilla fruticosa)on ecosystem CO_(2) fluxes in an alpine shrub meadow显示文摘Aims Recent studies have shown that alpine meadows on the Qinghai-Tibetan plateau act as significant CO_(2)sinks.On the plateau,alpine shrub meadow is one of typical grassland ecosystems.The major alpine shrub on the plateau is Potentilla fruticosa L.(Rosaceae),which is distributed widely from 3200 to 4000 m.Shrub species play an important role on carbon sequestration in grassland ecosystems.In addition,alpine shrubs are sensitive to climate change such as global warming.Considering global warming,the biomass and productivity of P.fruticosa will increase on Qinghai-Tibetan Plateau.Thus,understanding the carbon dynamics in alpine shrub meadow and the role of shrubs around the upper distribution limit at present is essential to predict the change in carbon sequestration on the plateau.However,the role of shrubs on the carbon dynamics in alpine shrub meadow remains unclear.The objectives of the present study were to evaluate the magnitude of CO_(2)exchange of P.fruticosa shrub patches around the upper distribution limit and to elucidate the role of P.fruticosa on ecosystem CO_(2)fluxes in an alpine meadow.Methods We used the static acrylic chamber technique to measure and estimate the net ecosystem productivity(NEP),ecosystem respiration(Re),and gross primary productivity(GPP)of P.fruticosa shrub patches at three elevations around the species’upper distribution limit.Ecosystem CO_(2)fluxes and environmental factors were measured from 17 to 20 July 2008 at 3400,3600,and 3800 m a.s.l.We examined the maximum GPP at infinite light(GPPmax)and maximum Re(Remax)during the experimental time at each elevation in relation to aboveground biomass and environmental factors,including air and soil temperature,and soil water content.Important Findings Patches of P.fruticosa around the species’upper distribution limit absorbed CO_(2),at least during the daytime.Maximum NEP at infinite light(NEPmax)and GPPmax of shrub patches in the alpine meadow varied among the three elevations,with the highest values at 3400 m and the lowest at 3800 m.GPPmax was positively correlated with the green biomass of P.fruticosa more strongly than with total green biomass,suggesting that P.fruticosa is the major contributor to CO_(2)uptake in the alpine shrub meadow.Air temperature influenced the potential GPPat the shrub-patch scale.Remax was correlated with aboveground biomass and Remax normalized by aboveground biomass was influenced by soil water content.Potentilla fruticosa height(biomass)and frequency increased clearly as elevation decreased,which promotes the large-scale spatial variation of carbon uptake and the strength of the carbon sink at lower elevations. | Yuichiro Yashiro Yoko Shizu Mitsuru Hirota Ayako Shimono Toshiyuki Ohtsuka | 2010 | Journal of Plant Ecology2010，3，2： | 2 |
| 17 | High-temperature strength characterization of advanced 9Cr-ODS ferritic steels显示文摘 | S. Ukai S. Ohtsuka T. Kaito H. Sakasegawa N. Chikata S. Hayashi S. Ohnuki | 2009 | Materials Science & Engineering A2009，，： | 2 |
| 18 | PiggyBac transposon-mediated gene delivery efficiently generates stable transfectants derived from cultured primary human deciduous tooth dental pulp cells(HDDPCs) and HDDPC-derived iPS cells显示文摘The ability of human deciduous tooth dental pulp cells(HDDPCs) to differentiate into odontoblasts that generate mineralized tissue holds immense potential for therapeutic use in the field of tooth regenerative medicine. Realization of this potential depends on efficient and optimized protocols for the genetic manipulation of HDDPCs. In this study, we demonstrate the use of a Piggy Bac(PB)-based gene transfer system as a method for introducing nonviral transposon DNA into HDDPCs and HDDPC-derived inducible pluripotent stem cells. The transfection efficiency of the PB-based system was significantly greater than previously reported for electroporation-based transfection of plasmid DNA. Using the neomycin resistance gene as a selection marker, HDDPCs were stably transfected at a rate nearly 40-fold higher than that achieved using conventional methods. Using this system, it was also possible to introduce two constructs simultaneously into a single cell. The resulting stable transfectants, expressing td Tomato and enhanced green fluorescent protein, exhibited both red and green fluorescence. The established cell line did not lose the acquired phenotype over three months of culture. Based on our results, we concluded that PB is superior to currently available methods for introducing plasmid DNA into HDDPCs. There may be significant challenges in the direct clinical application of this method for human dental tissue engineering due to safety risks and ethical concerns. However, the high level of transfection achieved with PB may have significant advantages in basic scientific research for dental tissue engineering applications, such as functional studies of genes and proteins. Furthermore, it is a useful tool for the isolation of genetically engineered HDDPC-derived stem cells for studies in tooth regenerative medicine. | Emi Inada Issei Saitoh Satoshi Watanabe Reiji Aoki Hiromi Miura Masato Ohtsuka Tomoya Murakami Tadashi Sawami Youichi Yamasaki Masahiro Sato | 2015 | International Journal of Oral Science2015，7，3： | 2 |
| 19 | Blinded nonrandomized comparative study of gastric examination with a magnetically guided capsule endoscope and standard videoendoscope显示文摘 | Jean-Francois Rey Haruhiko Ogata Naoki Hosoe Kazuo Ohtsuka Noriyuki Ogata Keiichi Ikeda Hiroyuki Aihara Ileana Pangtay Toshifumi Hibi Shin-Ei Kudo Hisao Tajiri | 2012 | Gastrointestinal Endoscopy2012，，2： | 2 |
| 20 | Helicobacter pylori infection and gastropathy:A comparison between Indonesian and Japanese patients显示文摘AIM:To compare the effects of Helicobacter pylori(H pylori) infection on gastropathy between Indonesian and Japanese patients.METHODS:Biopsy specimens were obtained during upper gastrointestinal endoscopy from 167 subjects(125 Indonesians and 42 Japanese) with uninvestigated symptoms of dyspepsia.The specimens were analyzed for the presence of H pylori using urease analysis,histopathology,and cell culture.The grade and activity of gastritis was assessed using the updated Sydney system.RESULTS:The percentages of Indonesian and Japanese patients who were H pylori-positive at the antrum or body of the stomach were similar(68% and 59.5%,respectively;P=0.316).Of those who were H pylori-positive,more Japanese patients than Indonesian patients had high levels of polymorphonuclear cells(P=0.001),mononuclear cells(P=0.013),glandular atrophy(P=0.000),and intestinal metaplasia(P=0.011) in both the antrum and body of the stomach.CONCLUSION:The grade of gastritis and prevalence of mucosal atrophy and intestinal metaplasia were higher in Japanese patients.The difference between Indonesian and Japanese patients was signifi cant. | Murdani Abdullah Hiroyuki Ohtsuka Abdul Aziz Rani Tadashi Sato Ari F Syam Masayuki A Fujino | 2009 | World Journal of Gastroenterology2009，15，39： | 2 |
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