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| 1 | 地被菊花Fall Color体细胞胚途径再生、遗传转化及转基因植株的抗寒性检测显示文摘【目的】培育耐寒性强的地被菊花[Dendranthemagrandiflorum(Ramat.)Kitamura]新材料。【方法】以FallColor品种的幼嫩叶片为外植体,探讨胚状体诱导所需的植物生长调节剂浓度和诱导培养时间等条件。【结果】叶片外植体在添加0.75mg·L-12,4-D的诱导培养基上诱导15d,再进行分生培养,不仅能够诱导胚性愈伤组织形成,还能够诱导胚状体发生,并进一步诱导芽再生,最终93%的供试外植体通过胚状体途径获得芽的再生。通过根癌农杆菌介导法将35S启动子驱动的逆境诱导转录因子DREB1A基因导入该品种。转化株在低温下的种子发芽率、扦插苗生长以及植株露地越冬生长状况等方面都明显优于对照。【结论】本研究成功地建立了地被菊花FallColor体细胞胚再生途径,并成功地获得了具有越冬耐性的地被菊花转化株系。 | 洪波 仝征 李邱华 马超 KASUGA Mie YAMAGUCHI-SHINOZA KIKaziko 高俊平 | 2006 | 中国农业科学2006，39，7： | 28 |
| 2 | AtDREB1A基因在菊花中的异源表达提高了植株对干旱和盐渍胁迫的耐性显示文摘将携带有AtDREBlA基因,并以35S或rd29A启动子驱动的植物表达载体转入地被菊花(Dendranthema grandiflorum)的粉色品种‘Fall color’.与野生型相比,35S:DREBIA转基因植株表现出严重的生长抑制,而rd29A:DREB1A植株生长正常.RT-PCR检测表明,在胁迫条件下, AtDREB1A基因在35S:DREB1A转基因植株中呈现组成型过量表达,而在rd29A:DREB1A植株中则是受胁迫诱导型过量表达.这两种启动子驱动的转基因植株对干旱和盐溃胁迫都表现出较强的耐性,其中rd29A:DREB1A植株耐性显著强于35S:DREB1A植株.rd29A:DREB1A植株中的脯氨酸含量和SOD活性都强烈地被胁迫诱导升高,且高于35S:DREB1A植株.这些结果表明,在地被菊花中表达AtDREB1A基因可以提高植株对干旱和盐渍胁迫的耐性,同时这些耐性的升高可能与脯氨酸含量和SOD活性的上升有关. | 洪波 仝征 马男 李建科 Mie Kasuga Kazuko Yamaguchi-Shinozaki 高俊平 | 2006 | 中国科学(C辑)2006，36，3： | 25 |
| 3 | 根癌农杆菌介导的转录因子DREB1A基因在地被菊花中的遗传转化显示文摘以地被菊花(Dendranthema grandiflorum cv.White Snow)无菌苗叶片为外植体,在附加不同浓度植物生长调节剂的MS基础培养基上诱导培养,通过器官直接发生途径获得再生植株。结果表明,2.0mg/L6-BA和0.2mg/LNAA组合可获得93.8%的再生芽。将含拟南芥(Arabidopsis thaliana)逆境诱导转录因子DREB1A基因的植物表达载体pBI-DREB1A导入根癌农杆菌(Agrobacterium tumefaciens)LBA4404,农杆菌介导法遗传转化地被菊花White Snow叶盘,经PCR和PCR-Southern检测,DREB1A基因已整合到转化植株的基因组中。遗传转化过程中,预培养2d后,将OD600=0.5~0.7的根癌农杆菌稀释30倍后侵染叶盘10min,再共培养2d,有利于获得最高遗传转化效率。 | 洪波 张常青 李邱华 高俊平 KASUGA Mie SHINOZAKI-YAMAGUCHI Kazuko | 2005 | 农业生物技术学报2005，13，3： | 20 |
| 4 | Heterologous expression of the AtDREB1A gene in chrysanthemum increases drought and salt stress tolerance显示文摘DNA cassette containing an AtDREB1A cDNA and a nos terminator,driven by a cauli- flower mosaic 35S promoter,or a stress-inducible rd29A promoter,was transformed into the ground cover chrysanthemum(Dendranthema grandiflorum)'Fall Color'genome.Compared with wild type plants,severe growth retardation was observed in 35S:DREB1A plants,but not in rd29A:DREB1A plants.RT-PCR analysis revealed that,under stress conditions,the DREB1A gene was over-expressed constitutively in 35S:DREB1A plants,but was over-expressed inductively in rd29A:DREB1A plants.The transgenic plants exhibited tolerance to drought and salt stress,and the tolerance was significantly stronger in rd29A:DREB1A plants than in 35S:DREB1A plants.Proline content and SOD activity were increased inductively in rd29A:DREB1A plants than in 35S:DREB1A plants under stress conditions.These results indicate that heterologous AtDREB1A can confer drought and salt tolerance in transgenic chrysanthemum,and improvement of the stress tolerance may be related to enhancement of proline content and SOD activity. | KASUGA Mie YAMAGUCHI-SHINOZAKI Kazuko | 2006 | Science China(Life Sciences)2006，49，5： | 18 |
| 5 | Comprehensive and innovative techniques for livertransplantation in rats: A surgical guide显示文摘AIM: To investigate our learning curves of orthotopic liver transplantation (OLT) in rats and the most important factor for successful surgery. METHODS: We describe the surgical procedures for our rat OLT model, and determined the operator learning curves. The various factors that contributed to successful surgery were determined. The most important surgical factors were evaluated between successful and unsuccessful surgeries.RESULTS: Learning curve data indicated that 50 cases were required for operator training to start a study. Operative time, blood loss, warm ischemic time, anhepatic phase, unstable systemic hemodynamic state, and body temperature after surgery significantly affected surgery success by univariate analysis, while the anhepatic phase was the most critical factor for success by multivariate analysis. CONCLUSION: OLT in rats is the only liver transplantation model that provides clinically relevant and reliable results. Shortened anhepatic phase is key to success in this model. | Tomohide Hori Justin H Nguyen Yasuhiro Ogura Toshiyuki Hata Shintaro Yagi Ann-Marie T Baine Norifumi Ohashi Christopher B Eckman Aimee R Herdt Hiroto Egawa Yasutsugu Takada Fumitaka Oike Seisuke Saka-moto Mureo Kasahara Kohei Ogawa Koichiro Hata Taku Iida Yukihide Yonekawa Lena Sibulesky Kagemasa Kuribayashi Takuma Kato Kanako Saito Mie Torii Naruhiko Sahara Naoko Kamo Tomoko Sahara Motohiko Yasutomi Shinji Uemoto | 2010 | World Journal of Gastroenterology2010，16，25： | 14 |
| 6 | Usefulness of anti-ulcer drugs for the prevention and treatment of peptic ulcers induced by low doses of aspirin显示文摘AIM:To investigate the usefulness of anti-ulcer drugs for the prevention and treatment of low-dose aspirin- induced peptic ulcer. METHODS:Upper gastrointestinal endoscopy was performed in 68 patients receiving daily low-dose aspirin(81 or 100 mg/day).The endoscopic findings were classified according to the Lanza score,and the scores were compared between groups categorized according to the concomitant use of anti-ulcer drugs and the types of drugs used.In another study,31 hemorrhagic peptic ulcer patients who had been receiving low-dose aspirin were enrolled.The patients were randomly classified into the proton pump inhibitor (PPI)-treated group and the H2 receptor antagonist (H2RA)-treated group.The administration of low-dose aspirin was continued concomitantly,and endoscopic examinations were performed 8 wk later. RESULTS:The Lanza scores(mean±SD)of the gastro-mucosal lesions were 1.0±1.9 and 1.9±2.3 in 8 and 16 patients receiving prevention therapy with a PPI and an H2RA,respectively.Both scores were significantly smaller than the scores in 34 patients who were not receiving prevention therapy(4.7±1.0)and in 10 patients receiving cytoprotective anti-ulcer drugs (4.3±1.6).In the prospective study,18 and 13 patients received a PPI and an H2RA,respectively.Endoscopic examinations revealed that the tissue in the region of the gastro-mucosal lesions had reverted to normal in all patients in the PPI-treated group and in 12 patients (92%)in the H2RA-treated group;no significant differences were observed between the groups. CONCLUSION:H2RA therapy was effective for both the prevention and treatment of low-dose aspirin- induced peptic ulcer,similar to the effects of PPIs, while cytoprotective anti-ulcer drugs were ineffective in preventing ulceration. | Sayaka Nakashima Shinichi Ota Shin Arai Kiyoko Yoshino Mie Inao Keiko Ishikawa Nobuaki Nakayama Yukinori Imai Sumiko Nagoshi Satoshi Mochida | 2009 | World Journal of Gastroenterology2009，15，6： | 11 |
| 7 | AU-rich element-binding proteins in colorectal cancer显示文摘Trans-acting factors controlling mRNA fate are critical for the post-transcriptional regulation of inflammation-related genes, as well as for oncogene and tumor suppressor expression in human cancers. Among them, a group of RNA-binding proteins called 'Adenylate-Uridylate-rich elements binding proteins'(AUBPs)control mRNA stability or translation through their binding to AU-rich elements enriched in the 3'UTRs of inflammation-and cancer-associated mRNA transcripts. AUBPs play a central role in the recruitment of target mRNAs into small cytoplasmic foci called Processing-bodies and stress granules(also known as P-body/SG). Alterations in the expression and activities of AUBPs and Pbody/SG assembly have been observed to occur with colorectal cancer(CRC)progression, indicating the significant role AUBP-dependent post-transcriptional regulation plays in controlling gene expression during CRC tumorigenesis.Accordingly, these alterations contribute to the pathological expression of many early-response genes involved in prostaglandin biosynthesis and inflammation,along with key oncogenic pathways. In this review, we summarize the current role of these proteins in CRC development. CRC remains a major cause of cancer mortality worldwide and, therefore, targeting these AUBPs to restore efficient post-transcriptional regulation of gene expression may represent an appealing therapeutic strategy. | Noémie Legrand Dan A Dixon Cyril Sobolewski | 2019 | World Journal of Gastrointestinal Oncology2019，11，2： | 7 |
| 8 | Cirrhotic patients and older people显示文摘The global population is aging,and so the number of older cirrhotic patients is increasing.Older patients are characterised by a risk of frailty and comorbidities,and age is a risk factor for mortality in cirrhotic patients.The incidence of nonalcoholic fatty liver disease as an aetiology of cirrhosis is increasing,while that of chronic viral hepatitis is decreasing.Also,cirrhosis is frequently idiopathic.The management of portal hypertension in older cirrhotic patients is similar to that in younger patients,despite the greater risk of treatment-related adverse events of the former.The prevalence of hepatocellular carcinoma increases with age,but its treatment is unaffected.Liver transplantation is generally recommended for patients<70 years of age.Despite the increasing prevalence of cirrhosis in older people,little data are available and few recommendations have been proposed.This review suggests that comorbidities have a considerable impact on older cirrhotic patients. | Paul Carrier Marilyne Debette-Gratien Jérémie Jacques Véronique Loustaud-Ratti | 2019 | World Journal of Hepatology2019，11，9： | 6 |
| 9 | Stability of cirrhotic systemic hemodynamics ensures sufficient splanchnic blood flow after living-donor liver transplantation in adult recipients with liver cirrhosis显示文摘AIM: To investigate the correlation between systemic hemodynamics and splanchnic circulation in recipients with cirrhosis undergoing living-donor liver transplantation (LDLT), and to clarify how systemic hemodynamics impact on local graft circulation after LDLT. METHODS: Systemic hemodynamics, indocyanine green (ICG) elimination rate (KICG) and splanchnic circulation were simultaneously and non-invasively investigated by pulse dye densitometry (PDD) and ultrasound. Accurate estimators of optimal systemic hyperdynamics after LDLT [i.e., balance of cardiac output (CO) to blood volume (BV) and mean transit time (MTT), defined as the timerequired for half the administered ICG to pass through an attached PDD sensor in the first circulation] were also measured. Thirty recipients with cirrhosis were divided into two groups based on clinical outcomes corresponding to postoperative graft function. RESULTS: Cirrhotic systemic hyperdynamics characterized by high CO, expanded BV and low total peripheral resistance (TPR) were observed before LDLT. TPR reflecting cirrhotic vascular alterations was slowly restored after LDLT in both groups. Although no significant temporal differences in TPR were detected between the two groups, CO/BV and MTT differed significantly. Recipients with good outcomes showed persistent cirrhotic systemic hyperdynamics after LDLT, whereas recipients with poor outcomes presented with unstable cirrhotic systemic hyperdynamics and severely decreased KICG. Systemic hyperdynamic disorders after LDLT impacted on portal venous flow but not hepatic arterial flow. CONCLUSION: We conclude that subtle systemic hyperdynamics disorders impact on splanchnic circulation, and that an imbalance between CO and BV decreases portal venous flow, which results in critical outcomes. | Tomohide Hori Shintaro Yagi Taku Iida Kentaro Taniguchi Kentaro Yamagiwa Chiduru Yamamoto Takashi Hasegawa Koichiro Yamakado Takuma Kato Kanako Saito Linan Wang Mie Torii Yukinobu Hori Kan Takeda Kazuo Maruyama Shinji Uemoto | 2007 | World Journal of Gastroenterology2007，13，44： | 5 |
| 10 | 韩国老年人辅助器具与标准化显示文摘与亚洲其他国家相似,韩国也正步入老龄化社会。针对老年人的社会福利体系、人力资源以及相关辅助器具是保持社会健康和生产力的要素。本文介绍了韩国老年人辅助器具与标准的现状,分析了韩国老年人产业、长期护理保险计划和其他福利计划的现状,并讨论了老年人辅助器具的范畴及标准化的现状。 | Kyuyeoun Lee HyunKyoon Lim Mie Choi Hyejin Lee Nora Lee 王保华 何川 | 2011 | 中国康复理论与实践2011，17，6： | 4 |
| 11 | Elastic modulus of claystone evaluated by nano-/micro-indentation tests and meso-compression tests显示文摘Toarcian claystone such as that of the Callovo-Oxfordian is a qualified multiphase material. The claystone samples tested in this study are composed of four main mineral phases: silicates(clay minerals, quartz,feldspars, micas)(z86%), sulphides(pyrite)(z3%), carbonates(calcite, dolomite)(z10%) and organic kerogen(z1%). Three sets of measurements of the modulus of deformability were compared as determined in(i) nanoindentation tests with a constant indentation depth of 2 mm,(ii) micro-indentation tests with a constant indentation depth of 20 mm, and(iii) meso-compression tests with a constant displacement of 200 mm. These three experimental methods have already been validated in earlier studies. The main objective of this study is to demonstrate the influence of the scaling effect on the modulus of deformability of the material. Different frequency distributions of the modulus of deformability were obtained at the different sample scales:(i) in nano-indentation tests, the distribution was spread between 15 GPa and 90 GPa and contained one peak at34 GPa and another at 51 GPa;(ii) in the micro-indentation tests, the distribution was spread between 25 GPa and 60 GPa and displayed peaks at 26 GPa and 37 GPa; and(iii) in the meso-compression tests, a narrow frequency distribution was obtained, ranging from 25 GPa to 50 GPa and with a maximum at around 35 GPa. | Christophe Auvray Noémie Lafrance Danièle Bartier | 2017 | Journal of Rock Mechanics and Geotechnical Engineering2017，9，1： | 4 |
| 12 | Stress granules in colorectal cancer:Current knowledge and potential therapeutic applications显示文摘Stress granules(SGs)represent important non-membrane cytoplasmic compartments,involved in cellular adaptation to various stressful conditions(e.g.,hypoxia,nutrient deprivation,oxidative stress).These granules contain several scaffold proteins and RNA-binding proteins,which bind to mRNAs and keep them translationally silent while protecting them from harmful conditions.Although the role of SGs in cancer development is still poorly known and vary between cancer types,increasing evidence indicate that the expression and/or the activity of several key SGs components are deregulated in colorectal tumors but also in pre-neoplastic conditions(e.g.,inflammatory bowel disease),thus suggesting a potential role in the onset of colorectal cancer(CRC).It is therefore believed that SGs formation importantly contributes to various steps of colorectal tumorigenesis but also in chemoresistance.As CRC is the third most frequent cancer and one of the leading causes of cancer mortality worldwide,development of new therapeutic targets is needed to offset the development of chemoresistance and formation of metastasis.Abolishing SGs assembly may therefore represent an appealing therapeutic strategy to re-sensitize colon cancer cells to anti-cancer chemotherapies.In this review,we summarize the current knowledge on SGs in colorectal cancer and the potential therapeutic strategies that could be employed to target them. | Noémie Legrand Dan A Dixon Cyril Sobolewski | 2020 | World Journal of Gastroenterology2020，26，35： | 4 |
| 13 | Spatial and temporal patterns of drought in Zambia显示文摘Drought acutely affects economic sectors, natural habitats and communities. Understanding the past spatial and temporal patterns of drought is crucial because it facilitates the forecasting of future drought occurrences and informs decision-making processes for possible adaptive measures. This is especially important in view of a changing climate. This study employed the World Meteorological Organization(WMO)-recommended standardized precipitation index(SPI) to investigate the spatial and temporal patterns of drought in Zambia from 1960 to 2016. The relationship between the occurrence of consecutive dry days(CDD; consecutive days with less than 1 mm of precipitation) and SPI was also investigated. Horizontal wind vectors at 850 hPa during the core of the rainy season(December–February)were examined to ascertain the patterns of flow during years of extreme and severe drought; and these were contrasted with the patterns of flow in 2007, which was a generally wet year. Pressure vertical velocity was also investigated. Based on the gamma distribution, SPI successfully categorized extremely dry(with a SPI value less than or equal to –2.0) years over Zambia as 1992 and 2015, a severely dry(–1.9 to –1.5) year as 1995, moderately dry(–1.4 to –1.0) years as 1972, 1980, 1987, 1999 and 2005, and 26 near normal years(–0.9 to 0.9). The occurrence of CDD was found to be strongly negatively correlated with SPI with a coefficient of –0.6. Further results suggest that, during wet years, Zambia is influenced by a clockwise circulating low-pressure zone over the south-eastern Angola, a second such zone over the northern and eastern parts, and a third over the Indian Ocean. In stark contrast, years of drought were characterized by an anti-clockwise circulating high-pressure zone over the south-western parts of Zambia,constraining precipitation activities over the country. Further, wet years were characterized by negative pressure vertical velocity anomalies, signifying ascending motion; while drought years were dominated by positive anomalies, signifying descending motion, which suppresses precipitation. These patterns can be used to forecast drought over Zambia and aid in strategic planning to limit the potential damage of drought. | Brigadier LIBANDA ZHENG Mie Chilekana NGONGA | 2019 | Journal of Arid Land2019，11，2： | 3 |
| 14 | Ultraviolet-induced alloantigen-specific immunosuppression in transplant immunity显示文摘After the first observation of the immunosuppressive effects of ultraviolet(UV) irradiation was reported in 1974, therapeutic modification of immune responses by UV irradiation began to be investigated in the context immunization. UV-induced immunosuppression is via the action of regulatory T cells(Tregs). Antigen-specific Tregs were induced by high-dose UV-B irradiation before antigen immunization in many studies, as it was considered that functional alteration and/or modulation of antigen-presenting cells by UV irradiation was required for the induction of antigen-specific immunosuppression. However, it is also reported that UV irradiation after immunization induces antigen-specific Tregs. UV-induced Tregs are also dominantly transferable, with interleukin-10 being important for UV-induced immunosuppression. Currently, various possible mechanisms involving Treg phenotype and cytokine profile have been suggested. UV irradiation accompanied by alloantigen immunization induces alloantigen-specific transferable Tregs, which have potential therapeutic applications in the transplantation field. Here we review the current status of UV-induced antigen-specific immunosuppression on the 40th anniversary of its discovery. | Tomohide Hori Kagemasa Kuribayashi Kanako Saito Linan Wang Mie Torii Shinji Uemoto Taku Iida Shintaro Yagi Takuma Kato | 2015 | World Journal of Transplantation2015，5，1： | 3 |
| 15 | Anti-amyloidogenic effects of soybean isoflavones in vitro : Fluorescence spectroscopy demonstrating direct binding to Aβ monomers, oligomers and fibrils显示文摘 | Mie Hirohata Kenjiro Ono Jun-ichi Takasaki Ryoichi Takahashi Tokuhei Ikeda Akiyoshi Morinaga Masahito Yamada | 2012 | BBA - Molecular Basis of Disease2012，，8： | 3 |
| 16 | Ribavirin:Past,present and future显示文摘Before the advent of direct acting antiviral agents(DAAs) ribavirin, associated to pegylated-interferon played a crucial role in the treatment of chronic hepatitis C, preventing relapses and breakthroughs. In the present era of new potent DAAs, a place is still devoted to the drug. Ribavirin associated with sofosbuvir alone is efficient in the treatment of most cases of G2 infected patients. All options currently available for the last difficult-to-treat cirrhotic G3 patients contain ribavirin. Reducing treatment duration to 12 wk in G1 or G4 cirrhotic compensated patients is feasible thanks to ribavirin. Retreating patients with acquired anti NS5 A resistance-associated variants using ribavirin-based strategies could be useful. The addition of ribavirin with DAAs combinations however, leads to more frequent but mild adverse events especially in cirrhotic patients. Preliminary data with interferon-free second generation DAAs combinations without ribavirin suggest that future of the drug is jeopardized even in difficult-totreat patients: The optimization of ribavirin dosage according to an early monitoring of blood levels has been suggested to be relevant in double therapy with peginterferon or sofosbuvir but not with very potent combinations of more than two DAAs. | Véronique Loustaud-Ratti Marilyne Debette-Gratien Jérémie Jacques Sophie Alain Pierre Marquet DenisSautereau Annick Rousseau Paul Carrier | 2016 | World Journal of Hepatology2016，8，2： | 3 |
| 17 | Environmental impacts of steel slag reused in road construction: A crystallographic and molecular (XANES) approach显示文摘 | Perrine Chaurand Jerome Rose Valérie Briois Luca Olivi Jean-Louis Hazemann Olivier Proux Jérémie Domas Jean-Yves Bottero | 2006 | Journal of Hazardous Materials2006，，3： | 2 |
| 18 | Heterostructured ZnFe_2O_4/Fe_2TiO_5/TiO_2 Composite Nanotube Arrays with an Improved Photocatalysis Degradation Efficiency Under Simulated Sunlight Irradiation显示文摘To improve the visible light absorption and photocatalytic activity of titanium dioxide nanotube arrays(TONTAs), ZnFe_2O_4(ZFO) nanocrystals were perfused into pristine TONTA pipelines using a novel bias voltageassisted perfusion method. ZFO nanocrystals were well anchored on the inner walls of the pristine TONTAs when the ZFO suspensions(0.025 mg m L^(-1)) were kept under a60 V bias voltage for 1 h. After annealing at 750 °C for2 h, the heterostructured ZFO/Fe_2 TiO_5(FTO)/TiO_2 composite nanotube arrays were successfully obtained. Furthermore, Fe^(3+)was reduced to Fe^(2+)when solid solution reactions occurred at the interface of ZFO and the pristine TONTAs. Introducing ZFO significantly enhanced thevisible light absorption of the ZFO/FTO/TONTAs relative to that of the annealed TONTAs. The coexistence of type I and staggered type II band alignment in the ZFO/FTO/TONTAs facilitated the separation of photogenerated electrons and holes, thereby improving the efficiency of the ZFO/FTO/TONTAs for photocatalytic degradation of methylene blue when irradiated with simulated sunlight. | Kun Xiong Kunzhou Wang Lin Chen Xinqing Wang Qingbo Fan Jérémie Courtois Yuliang Liu Xianguo Tuo Minhao Yan | 2018 | Nano-Micro Letters2018，10，1： | 2 |
| 19 | piRNAs Can Trigger a Multigenerational Epigenetic Memory in the Germline of C. elegans显示文摘 | Alyson Ashe Alexandra Sapetschnig Eva-Maria Weick Jacinth Mitchell Marloes P. Bagijn Amy C. Cording Anna-Lisa Doebley Leonard D. Goldstein Nicolas J. Lehrbach Jérémie Le Pen Greta Pintacuda Aisa Sakaguchi Peter Sarkies Shawn Ahmed Eric A. Miska | 2012 | Cell2012，，1： | 2 |
| 20 | 论高掺量粉煤灰烧结砖显示文摘作者依据近年设计与施工实践,结合调研,面向墙材革新现实,就高掺量粉煤灰烧结砖的发展趋势作一浅析. | mie建华 | 2002 | 粉煤灰综合利用2002，16，3： | 2 |
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