|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Lectin receptor kinase OsLecRK-S.7 is required for pollen development and male fertility显示文摘Pollen grains are covered by exine that protects the pollen from stress and facilitates pollination.Here we isolated a male sterile mutant s13283 in rice exhibiting aborted pollen with abnormal exine and defective aperture.The mutant gene encodes a novel plasma membrane-localized legume-lectin receptor kinase that we named OsLecRK-S.7.OsLecRK-S.7 was expressed at different levels in all tested tissues and throughout anther development.In vitro kinase assay showed OsLecRK-S.7 capable of autophosporylation.Mutation in s13283(E560K)and mutation of the conserved ATP binding site(K418E)both knocked out the kinase activity.Mass spectrometry showed Thr376,Ser378,Thr386,Thr403,and Thr657 to be the autophosphorylation sites.Mutation of individual autophosphorylation site affected the in vitro kinase activity to different degrees,but did not abolish the gene function in fertility complementation.oslecrk-s.7 mutant plant overexpressing OsLecRK-S.7 recovered male fertility but showed severe growth retardation with reduced number of tillers,and these phenotypes were abolished by E560K or K418E mutation.The results indicated that OsLecRK-S.7 was a key regulator of pollen development. | Xiaoqun Peng Menglong Wang Yiqi Li Wei Yan Zhenyi Chang Zhufeng Chen Chunjue Xu Chengwei Yang Xing Wang Deng Jianxin Wu Xiaoyan Tang | 2020 | Journal of Integrative Plant Biology2020，62，8： | 4 |
| 2 | Identification of late-stage pollen-specific promoters for construction of pollen-inactivation system in rice显示文摘Large-scale production of male sterile seeds can be achieved by introducing a fertility-restoration gene linked with a pollen-killer gene into a recessive male sterile mutant.We attempted to construct this system in rice by using a late-stage pollen-specific(LSP)promoter driving the expression of maizeα-amylase gene ZM-AA1.To obtain such promoters in rice,we conducted comparative RNA-seq analysis of mature pollen with meiosis anther,and compared this with the transcriptomic data of various tissues in the Rice Expression Database,resulting in 269 candidate LSP genes.Initial test of nine LSP genes showed that only the most active OsLSP3 promoter could drive ZM-AA1 to disrupt pollen.We then analyzed an additional 22 LSP genes and found 12 genes stronger than OsLSP3 in late-stage anthers.The promoters of OsLSP5 and OsLSP6 showing higher expression than OsLSP3 at stages 11 and 12 could drive ZM-AA1 to inactivate pollen,while the promoter of OsLSP4 showing higher expression at stage 12 only could not drive ZM-AA1 to disrupt pollen,suggesting that strong promoter activity at stage 11 was critical for pollen inactivation.The strong pollen-specific promoters identified in this study provided valuable tools for genetic engineering of rice male sterile system for hybrid rice production. | Menglong Wang Wei Yan Xiaoqun Peng Zhufeng Chen Chunjue Xu Jianxin Wu Xing Wang Deng Xiaoyan Tang | 2020 | Journal of Integrative Plant Biology2020，62，8： | 4 |
| 3 | GDSL esterase/lipases OsGELP34 and OsGELP110/OsGELP115 are essential for rice pollen development显示文摘Pollen exine contains complex biopolymers of aliphatic lipids and phenolics.Abnormal development of pollen exine often leads to plant sterility.Molecular mechanisms regulating exine formation have been studied extensively but remain ambiguous.Here we report the analyses of three GDSL esterase/lipase protein genes,OsGELP34,OsGELP110,and OsGELP115,for rice exine for-mation.OsGELP34 was identified by cloning of a male sterile mutant gene.OsGELP34 encodes an endoplasmic reticulum protein and was mainly expressed in anthers during pollen exine formation.osgelp34 mutant displayed abnormal exine and altered expression of a number of key genes required for pollen development.OsGELP110 was previously identified as a gene differentially expressed in meiotic anthers.OsGELP110 was most homologous to OsGELP115,and the two genes showed similar gene expression patterns.Both OsGELP110 and OsGELP115 proteins were localized in peroxisomes.Individual knockout of OsGELP110 and OsGELP115 did not affect the plant fertility,but double knockout of both genes altered the exine structure and rendered the plant male sterile.OsGELP34 is distant from OsGELP110 and OsGELP115 in sequence,and osgelp34 and osgelp110/osgelp115 mutants were different in anther morphology despite both were male sterile.These results suggested that OsGELP34 and OsGELP110/OsGELP115 catalyze different compounds for pollen exine development. | Huihui Zhang Menglong Wang Yiqi Li Wei Yan Zhenyi Chang Haoling Ni Zhufeng Chen Jianxin Wu Chunjue Xu Xing Wang Deng Xiaoyan Tang | 2020 | Journal of Integrative Plant Biology2020，62，10： | 4 |
| 4 | Physiological Changes and Antioxidative Mechanisms of Alternanthera philoxeroides in Phytoremediation of Cadmium显示文摘This study evaluated the physiological characteristics(e.g.,growth parameters,chlorophyll content,metabolites and antioxidative enzymes activity)of Alternanthera philoxeroides(A.philoxeroides),as a hyperaccumulator plant,during the phytoremediation of cadmium(Cd)from water.After cultivating A.philoxeroides in a Cd-containing medium for 30 days,the growth rate was inhibited by up to 33.5%as the exposed Cd concentration increased to 0.80 mmol·L−1.Cd exposure interfered with the photosynthesis of A.philoxeroides and caused oxidative stress as indicated by the rise of malondialdehyde(MDA)and H2O2,which increased by 8 times and 3 times compared to the control group.Moreover,high exposure concentrations of Cd also reduced the activities of multiple antioxidants(e.g.,GSH and AsA),indicating the inhibition of Cd on the plant’s ability to mitigate oxidative damage.Finally,the fluorescent patterns of the rhizosphere dissolved organic matter(rDOM)revealed three major components(humic,fulvic substances and protein-like substances)well correlated with the changes in antioxidant activities.Partial least-squares discriminant analysis(PLS-DA)visualized the difference in the activity of the antioxidative enzymes between different groups.The study unravelled deep insights into the potential mechanisms of tolerance and resistance of A.philoxeroides for phytoremediation of Cd pollution. | Dajiang Yan Shan Xue Zhibin Zhang Guodong Xu Yanhao Zhang Yanfeng Shi Menglong Xing Wen Zhang | 2023 | Environment & Health2023，1，2： | 0 |
| 5 | Synthesis and optimization of high-performance amine-based polymer for CO_(2) separation显示文摘Membrane technology features inspiring excellence from numerous separation technologies for CO_(2) capture from post-combustion gas.Polyvinylamine(PVAm)-based facilitated transport membranes show significantly high separation performance,which has been proven promising for industrial scale-up.However,commercialized PVAm with low molecular weight and excessive crystallinity is not available to prepare high-performance membranes.Herein,the synthesis process of PVAm was optimized by regulating polymerization and acidic hydrolytic conditions.The membranes based on PVAm with a molecular weight of 154 kDa and crystallinity of 11.37%display high CO_(2) permeance of 726 GPU and CO_(2)/N_(2) selectivity of 55 at a feed gas pressure of 0.50 MPa.Furthermore,we established a PVAm synthesis reactor with an annual PVAm solution(1%(mass))capacity of over 7000 kg and realized the scaled-up manufacture of both PVAm and composite membranes. | Ye Yuan Yurui Pan Menglong Sheng Guangyu Xing Ming Wang Jixiao Wang Zhi Wang | 2022 | Chinese Journal of Chemical Engineering2022，，10： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员