|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | TTYH2,a human homologue of the Drosophila melanogaster gene tweety,is up-regulated in colon carcinoma and involved in cell proliferation and cell aggregation显示文摘AIM: To investigate the expression patterns of TTYH2 in the human colon cancer and colon cancer cell lines and to evaluate the inhibitory effect of small interfering RNA (siRNA) on the expression of TTYH2 in colon cancer cell lines.METHODS: We investigated the expression patterns of TTYH2 in colon cancer, adjacent non-tumorous colon mucosa, and cancer cell lines (DLD-1, caco-2, and Lovo) by RT-PCR. Furthermore, a siRNA plasmid expression vector against TTYH2 was constructed and transfected into DLD-1 and Caco-2 with LipofectamineTM 2000. The down regulation of TTYH2 expression was detected by RT-PCR and the role of siRNA in inducing cell proliferation and cell aggregation was evaluated by MTT and aggregation assay.RESULTS: TTYH2 gene expression in colon cancer tissue was significantly up-regulated compared with normal colonic mucosa (1.23 ± 0.404 vs 0.655 ± 0.373, P = 0.0103). Colon cancer derived cell lines including DLD-1, Caco-2, and Lovo also expressed high levels of TTYH2. In contrast, transfection with siRNA-TTYH2 signifi cantly inhibited both proliferation and scattering of these cancer cell lines.CONCLUSION: The present work demonstrates, for the fi rst time, that the TTYH2 gene expression is signifi cantly up-regulated in colon cancer. The TTYH2 gene may play an important role in regulating both proliferating andmetastatic potentials of colorectal cancer. | Yuji Toiyama Akira Mizoguchi Kazushi Kimura Junichirou Hiro Yasuhiro Inoue Tomonari Tutumi Chikao Miki Masato Kusunoki | 2007 | World Journal of Gastroenterology2007，13，19： | 4 |
| 2 | Prognostic Significance of Host- and Tumor-Related Factors in Patients with Gastric Cancer显示文摘 | Yasuhiko Mohri Kouji Tanaka Masaki Ohi Takeshi Yokoe Chikao Miki Masato Kusunoki | 2010 | World Journal of Surgery2010，，2： | 3 |
| 3 | Prognostic Nutritional Index Predicts Postoperative Outcome in Colorectal Cancer显示文摘 | Yasuhiko Mohri Yasuhiro Inoue Koji Tanaka Junichirou Hiro Keiichi Uchida Masato Kusunoki | 2013 | World Journal of Surgery2013，，11： | 3 |
| 4 | Serum miR-200c Is a Novel Prognostic and Metastasis-Predictive Biomarker in Patients With Colorectal Cancer显示文摘 | Yuji Toiyama Keun Hur Koji Tanaka Yasuhiro Inoue Masato Kusunoki C. Richard Boland Ajay Goel | 2014 | Annals of Surgery2014，，4： | 3 |
| 5 | Correlation of CD133, OCT4, and SOX2 in Rectal Cancer and Their Association with Distant Recurrence After Chemoradiotherapy显示文摘 | Susumu Saigusa Koji Tanaka Yuji Toiyama Takeshi Yokoe Yoshinaga Okugawa Yasuhiro Ioue Chikao Miki Masato Kusunoki | 2009 | Annals of Surgical Oncology2009，，： | 2 |
| 6 | Persistence of gene expression changes in noninflamed and inflamed colonic mucosa in ulcerative colitis and their presence in colonic carcinoma显示文摘AIM: A few studies have applied genomic-wide gene expression analysis in inflamed colon tissue sample in ulcerative colitis (UC). We reported the first study of non-inflamed mucosal gene expression in UC and explored its clinical implication.METHODS: Non-inflamed mucosa was obtained from 6 UC patients who received total colectomy. The gene expression of UC in noninfliamed mucosa was monitored with a microarray. For a selected gene, RT-PCR was performed to verify array results and to further examine expression pattern in inflamed mucosa of UC, colorectal cancer tissue and normal mucosa using additional matched pairs.RESULTS: Two genes showing 2.5-fold decreased expression with significance set at in UC samples were bomeo box a4 (HOXa4) and mads box transcription enhancer factor 2, polypeptide B (MEF2b). RT-PCR showed that MEF2b expression in non-inflamed mucosa was significantly downregulated, whereas the expression of MEF2b increased in accordance with the severity of mucosal inflammation. HOXa4 expression both in inflamed and non-inflamed mucosa in UC was consistently downregulated, and the downregulation of HOXa4 was also found in colorectal carcinoma.CONCLUSION: It is suggested that the MEF2b expression in UC which increase in accordance with inflammation may be induced by the inflammatory mediator. In contrast the downregulation of HOXa4 may be partly involved in the pathogenesis of disease including UC and UC-related carcinogenesis. | Yuji Toiyama Akira Mizoguchi Kazushi Kimura Toshimitsu Araki Shigeyuki Yoshiyama Kouhei Sakaguchi Chikao Miki Masato Kusunoki | 2005 | World Journal of Gastroenterology2005，11，33： | 2 |
| 7 | Correlation of CD133, OCT4, and SOX2 in Rectal Cancer and Their Association with Distant Recurrence After Chemoradiotherapy显示文摘 | Susumu Saigusa MD Koji Tanaka MD Yuji Toiyama MD Takeshi Yokoe MD Yoshinaga Okugawa MD Yasuhiro Ioue MD PhD Chikao Miki MD PhD Masato Kusunoki MD PhD | 2009 | Annals of Surgical Oncology2009，，12： | 2 |
| 8 | Single‐incision laparoscopic and endoscopic cooperative surgery for gastrointestinal stromal tumor arising from the duodenum显示文摘 | Masaki Ohi Hiromi Yasuda Yoshito Ishino Masaki Katsurahara Susumu Saigusa Kyosuke Tanaka Koji Tanaka Yasuhiko Mohri Yasuhiro Inoue Keiichi Uchida Masato Kusunoki | 2013 | Asian J Endosc Surg2013，，4： | 2 |
| 9 | Correlation of CD133, OCT4, and SOX2 in Rectal Cancer and Their Association with Distant Recurrence After Chemoradiotherapy显示文摘 | Susumu Saigusa MD Koji Tanaka MD Yuji Toiyama MD Takeshi Yokoe MD Yoshinaga Okugawa MD Yasuhiro Ioue MD PhD Chikao Miki MD PhD Masato Kusunoki MD PhD | 2009 | Annals of Surgical Oncology2009，，12： | 1 |
| 10 | A large, solitary, semipedunculated gastric polyp in pediatric juvenile polyposis syndrome显示文摘 | Kohei Otake Keiichi Uchida Mikihiro Inoue Kohei Matsushita Kiyoshi Hashimoto Yuji Toiyama Koji Tanaka Kaname Nakatani Kazuo Kawai Masato Kusunoki | 2010 | Gastrointestinal Endoscopy2010，，6： | 1 |
| 11 | Correlation of CCL20 expression in rectal mucosa with the developmentof ulcerative colitis?associated neoplasia显示文摘 | Kiyoshi Hashimoto Susumu Saigusa Toshimitsu Araki Koji Tanaka Yoshiki Okita Hiroyuki Fujikawa Mikio Kawamura Yoshinaga Okugawa Yuji Toiyama Yasuhiro Inoue Keiichi Uchida Yasuhiko Mohri Masato Kusunoki | 2013 | Oncology Letters2013，，5： | 1 |
| 12 | Clinical Significance of Human Kallikrein7 Gene Expression in Colorectal Cancer显示文摘 | Yasuhiro Inoue Takehiko Yokobori Takeshi Yokoe Yuji Toiyama Chikao Miki Koshi Mimori Masaki Mori Masato Kusunoki | 2010 | Annals of Surgical Oncology2010，，11： | 1 |
| 13 | The Increased Expression of CCL20 and CCR6 in Rectal Mucosa Correlated to Severe Inflammation in Pediatric Ulcerative Colitis显示文摘 | Keiichi Uchida Yuhki Koike Kiyoshi Hashimoto Susumu Saigusa Mikihiro Inoue Kohei Otake Koji Tanaka Kohei Matsushita Yoshiki Okita Hiroyuki Fujikawa Toshimitsu Araki Yasuhiko Mohri Masato Kusunoki Paolo Gionchetti | 2015 | Gastroenterology Research and Practice2015，，： | 1 |
| 14 | High TrkB expression levels are associated with poor prognosis and EMT induction in colorectal cancer cells显示文摘 | Hiroyuki Fujikawa Koji Tanaka Yuji Toiyama Susumu Saigusa Yasuhiro Inoue Keiichi Uchida Masato Kusunoki | 2012 | Journal of Gastroenterology2012，，7： | 1 |
| 15 | Soluble CXCL16 in Preoperative Serum is a Novel Prognostic Marker and Predicts Recurrence of Liver Metastases in Colorectal Cancer Patients显示文摘 | Kohei Matsushita Yuji Toiyama Koji Tanaka Susumu Saigusa Junichiro Hiro Keiichi Uchida Yasuhiro Inoue Masato Kusunoki | 2012 | Annals of Surgical Oncology2012，，3： | 1 |
| 16 | Huge scrotal, flank, and retroperitoneal lymphangioma successfully treated by OK-432 sclerotherapy显示文摘 | Keiichi Uchida Mikihiro Inoue Toshimitsu Araki Chikao Miki Masato Kusunoki | 2002 | Urology2002，，6： | 1 |
| 17 | Intraoperative colonoscopy显示文摘 | Youichirou Sakanoue Koji Nakao Yasutsugu Shoji Hidenori Yanagi Masato Kusunoki Joji Utsunomiya | 1993 | Surgical Endoscopy1993，，2： | 1 |
| 18 | Cancer-associated fibroblasts correlate with poor prognosis in rectalcancer after chemoradiotherapy显示文摘 | Susumu Saigusa Yuji Toiyama Koji Tanaka Takeshi Yokoe Yoshinaga Okugawa Hiroyuki Fujikawa Kohei Matsusita Mikio Kawamura Yasuhiro Inoue Chikao Miki Masato Kusunoki | 2011 | International Journal of Oncology2011，，3： | 1 |
| 19 | Soluble CXCL16 in Preoperative Serum is a Novel Prognostic Marker and Predicts Recurrence of Liver Metastases in Colorectal Cancer Patients显示文摘 | Kohei Matsushita Yuji Toiyama Koji Tanaka Susumu Saigusa Junichiro Hiro Keiichi Uchida Yasuhiro Inoue Masato Kusunoki | 2012 | Annals of Surgical Oncology2012，，3： | 1 |
| 20 | Intraluminal appendiceal fluid is a predictive factor for recurrent appendicitis after initial successful non-operative management of uncomplicated appendicitis in pediatric patients显示文摘 | Yuhki Koike Keiichi Uchida Kohei Matsushita Kohei Otake Makoto Nakazawa Mikihiro Inoue Masato Kusunoki Yoshihide Tsukamoto | 2014 | Journal of Pediatric Surgery2014，，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员