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| 1 | A high-density, multi-parental SNP genetic map on apple validates a new mapping approach for outcrossing species显示文摘Quantitative trait loci(QTL)mapping approaches rely on the correct ordering of molecular markers along the chromosomes,which can be obtained from genetic linkage maps or a reference genome sequence.For apple(Malus domestica Borkh),the genome sequence v1 and v2 could not meet this need;therefore,a novel approach was devised to develop a dense genetic linkage map,providing the most reliable marker-loci order for the highest possible number of markers.The approach was based on four strategies:(i)the use of multiple full-sib families,(ii)the reduction of missing information through the use of HaploBlocks and alternative calling procedures for single-nucleotide polymorphism(SNP)markers,(iii)the construction of a single backcross-type data set including all families,and(iv)a two-step map generation procedure based on the sequential inclusion of markers.The map comprises 15417 SNP markers,clustered in 3 K HaploBlock markers spanning 1267 cM,with an average distance between adjacent markers of 0.37 cM and a maximum distance of 3.29 cM.Moreover,chromosome 5 was oriented according to its homoeologous chromosome 10.This map was useful to improve the apple genome sequence,design the Axiom Apple 480 K SNP array and perform multifamily-based QTL studies.Its collinearity with the genome sequences v1 and v3 are reported.To our knowledge,this is the shortest published SNP map in apple,while including the largest number of markers,families and individuals.This result validates our methodology,proving its value for the construction of integrated linkage maps for any outbreeding species. | Erica A Di Pierro Luca Gianfranceschi Mario Di Guardo Herma JJ Koehorst-van Putten Johannes W Kruisselbrink Sara Longhi Michela Troggio Luca Bianco Hélène Muranty Giulia Pagliarani Stefano Tartarini Thomas Letschka Lidia Lozano Luis Larisa Garkava-Gustavsson Diego Micheletti Marco CAM Bink Roeland E Voorrips Ebrahimi Aziz Riccardo Velasco François Laurens W Eric van de Weg | 2016 | Horticulture Research2016，3，1： | 3 |
| 2 | Prognostic factors for osteosarcoma of the extremity treated with neoadjuvant chemotherapy:15-year experience in789patients treated at a single institution显示文摘 | BACCI G LONGHI A VERSARI M | 2006 | Cancer2006，06，5： | 1 |
| 3 | Prognostic factors for osteosarcoma of the extremity treated with neoadjuvant chemotherapy: 15-year experience in 789 patients treated at a single institution显示文摘 | Bacci G Longhi A Versari M | 2006 | Cancer2006，106，5： | 1 |
| 4 | Prognostic factors for osteosarcoma of the extremity treated with neoadjuvant chemotherapy:15-year experience in 789 patients treated at a single institution显示文摘 | Bacci G Longhi A Versari M | 2006 | Cancer2006，106，5： | 1 |
| 5 | Nonmetastatic osteosarcoma of the extremity with pathologic fracture at presentation:Local and systemic control by amputation or limb salvage after preoperative chemotherapy显示文摘 | BACCI G FERRARI S LONGHI A | | 0，，04： | 1 |
| 6 | Autoimmune hepatitis显示文摘 | Michael A Heneghan Andrew D Yeoman Sumita Verma Alastair D Smith Maria Serena Longhi | 2013 | The Lancet . 2013 (9902)2013，，： | 1 |
| 7 | Prognostic factors in non- metastatic Ewing sarcoma tumor of bone : an analysis of 579 pa- tients treated at a single institution with adjuvant or neoadjuvant chemotherapy between 1972 and 1998显示文摘 | Bacci G Longhi A Ferrari S | 2006 | Acta Oncol2006，45，4： | 1 |
| 8 | Quantitative analysis of enalapril by ^1H NMR spectroscopy in tablets 显示文摘 | Zoppi A Linares M Longhi M | 2005 | J Pharm Biomed Anal2005，37，3： | 1 |
| 9 | Delayed methotrexate clearance in osteosarcoma patients treated with multiagent regimens of neoadjuvant chemotherapy显示文摘 | BACCI G FERRARI S LONGHI A | 2003 | Oncol Rep2003，10，4： | 1 |
| 10 | Adjuvant andneoadjuvant chemotherapy for osteosarcoma of the extremities:27 year experience at Rizzoli Italy显示文摘 | Bacci G Longhi A Fagioli F | 2005 | Eur J Caner2005，41，18： | 1 |
| 11 | Primary bone os-teosarcoma in the pediatricage:state of the art 显示文摘 | Longhi A Errani C De Paolis M | 2006 | Cancer Treatment Reviews2006，32，6： | 1 |
| 12 | Role of surgery in local treatment of Ewing's sarcoma of the extremities in patients undergoing adjuvant and neoadjuvant chemotherapy显示文摘 | Bacci G Ferrari S Longhi A | 2004 | Oncol Rep2004，11，1： | 1 |
| 13 | Second malignancy in 597 patients with Ewing sarcoma of bone treated at a single institution with adjuvant and neoadjuvant chemotherapy between 1972 and 1999 显示文摘 | Bacci G Longhi A Barbieri E | 2005 | J Pediatr Hematol Oncol2005，27，10： | 1 |
| 14 | Local recurrence and local control of nonmetastatic osteosarcoma of the extremities:a27-year experience in a single institution显示文摘 | BACCI G FORNI C LONGHI A | 2007 | J Surg Oncol2007，96，2： | 1 |
| 15 | Influence of local recurrence on survival in patients with extremity osteosarcoma treated with neoadjuvant chemotherapy:the experience of a single institution with44patients显示文摘 | BACCI G LONGHI A CESARI M | 2006 | Cancer2006，106，12： | 1 |
| 16 | Mechanism of residence of cytochrome b(5), a tail-anchored protein, in the endoplasmic reticulum显示文摘 | Pedrazzini E Villa A Longhi R | 2000 | J Cell Biol2000，148，5： | 1 |
| 17 | Prognostic factors in nonmetastatic Ewing' s sarcoma of bone treated with adjuvant chemotherapy: Analysis of 359 patients at the Istituto Ortopedico Rizzoli 显示文摘 | Bacci G Ferrari S Bertoni F Rimondini S Longhi A Bacchini P | 2000 | J Clin Oncol2000，18，1： | 1 |
| 18 | Adjuvant and neoadjuvant chemotherapy for colon cancer of the extremities: 27 year experi- ence at Rizzoli Institute, Italy 显示文摘 | Bacci G Longhi A Fagioli F | 2005 | Eur J Cancer2005，41，18： | 1 |
| 19 | Adjuvant and neoadjuvant chemotherapy for osteosarcoma of the extremities : 27-year expe- rience at Rizzoli Institute, Italy 显示文摘 | Bacci G Longhi A Fagioli F | 2005 | Eur J Cancer2005，41，18： | 1 |
| 20 | Glaucoma and quality of life显示文摘 | Zan longhi X Arnndd B Bechetoiue A | 2003 | Fr Ophthalmol2003，16，2： | 1 |
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