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| 1 | Apple whole genome sequences:recent advances and new prospects显示文摘In 2010,a major scientific milestone was achieved for tree fruit crops:publication of the first draft whole genome sequence(WGS)for apple(Malus domestica).This WGS,v1.0,was valuable as the initial reference for sequence information,fine mapping,gene discovery,variant discovery,and tool development.A new,high quality apple WGS,GDDH13 v1.1,was released in 2017 and now serves as the reference genome for apple.Over the past decade,these apple WGSs have had an enormous impact on our understanding of apple biological functioning,trait physiology and inheritance,leading to practical applications for improving this highly valued crop.Causal gene identities for phenotypes of fundamental and practical interest can today be discovered much more rapidly.Genome-wide polymorphisms at high genetic resolution are screened efficiently over hundreds to thousands of individuals with new insights into genetic relationships and pedigrees.High-density genetic maps are constructed efficiently and quantitative trait loci for valuable traits are readily associated with positional candidate genes and/or converted into diagnostic tests for breeders.We understand the species,geographical,and genomic origins of domesticated apple more precisely,as well as its relationship to wild relatives.The WGS has turbo-charged application of these classical research steps to crop improvement and drives innovative methods to achieve more durable,environmentally sound,productive,and consumer-desirable apple production.This review includes examples of basic and practical breakthroughs and challenges in using the apple WGSs.Recommendations for“what’s next”focus on necessary upgrades to the genome sequence data pool,as well as for use of the data,to reach new frontiers in genomics-based scientific understanding of apple. | Cameron P.Peace Luca Bianco Michela Troggio Eric van de Weg Nicholas P.Howard Amandine Cornille Charles-Eric Durel Sean Myles ZoëMigicovsky Robert J.Schaffer Evelyne Costes Gennaro Fazio Hisayo Yamane Steve van Nocker Chris Gottschalk Fabrizio Costa David Chagné Xinzhong Zhang Andrea Patocchi Susan E.Gardiner Craig Hardner Satish Kumar Francois Laurens Etienne Bucher Dorrie Main Sook Jung Stijn Vanderzande | 2019 | Horticulture Research2019，6，1： | 3 |
| 2 | A high-density, multi-parental SNP genetic map on apple validates a new mapping approach for outcrossing species显示文摘Quantitative trait loci(QTL)mapping approaches rely on the correct ordering of molecular markers along the chromosomes,which can be obtained from genetic linkage maps or a reference genome sequence.For apple(Malus domestica Borkh),the genome sequence v1 and v2 could not meet this need;therefore,a novel approach was devised to develop a dense genetic linkage map,providing the most reliable marker-loci order for the highest possible number of markers.The approach was based on four strategies:(i)the use of multiple full-sib families,(ii)the reduction of missing information through the use of HaploBlocks and alternative calling procedures for single-nucleotide polymorphism(SNP)markers,(iii)the construction of a single backcross-type data set including all families,and(iv)a two-step map generation procedure based on the sequential inclusion of markers.The map comprises 15417 SNP markers,clustered in 3 K HaploBlock markers spanning 1267 cM,with an average distance between adjacent markers of 0.37 cM and a maximum distance of 3.29 cM.Moreover,chromosome 5 was oriented according to its homoeologous chromosome 10.This map was useful to improve the apple genome sequence,design the Axiom Apple 480 K SNP array and perform multifamily-based QTL studies.Its collinearity with the genome sequences v1 and v3 are reported.To our knowledge,this is the shortest published SNP map in apple,while including the largest number of markers,families and individuals.This result validates our methodology,proving its value for the construction of integrated linkage maps for any outbreeding species. | Erica A Di Pierro Luca Gianfranceschi Mario Di Guardo Herma JJ Koehorst-van Putten Johannes W Kruisselbrink Sara Longhi Michela Troggio Luca Bianco Hélène Muranty Giulia Pagliarani Stefano Tartarini Thomas Letschka Lidia Lozano Luis Larisa Garkava-Gustavsson Diego Micheletti Marco CAM Bink Roeland E Voorrips Ebrahimi Aziz Riccardo Velasco François Laurens W Eric van de Weg | 2016 | Horticulture Research2016，3，1： | 3 |
| 3 | The role of EGF - related peptides in tumor growth 显示文摘 | Normanno N Bianco C De Luca A | 2001 | Front Biosci2001，6，5： | 1 |
| 4 | The role of EGF-related peptldes in tumor growth 显示文摘 | NORMANNO N BIANCO C DE LUCA A | 2001 | Front Biosci2001，6，1： | 1 |
| 5 | Target-based agents agonist ERB receptors and their ligand:a novel approach to cancer treatment 显示文摘 | Normanno N Bianco C De Luca A | 2003 | Endocr Relat Cancer2003，10，1： | 1 |
| 6 | Cfipto-1 overexpression leads to enhanced invasiveness and resistance to anoikis in humanMCF-7 breast cancer cells 显示文摘 | Normanno N De Luca A Bianco C | 2004 | J Cell Physiol2004，198，1： | 1 |
| 7 | Epidermal growth factor receptor (EGFR) signaling in cancer 显示文摘 | Normanno N De Luca A Bianco C | 2006 | Gene2006，366，1： | 1 |
| 8 | Cripto-1 overexpression leads to enhanced invasiveness and resistance to anoikis in human MCF-7 breast cancer cells显示文摘 | Normanno N De Luca A Bianco C | 2004 | J Cell Physiol2004，198，1： | 1 |
| 9 | The role of EGF-related peptides in tumor growth显示文摘 | Normanno N Bianco C De Luca A | 2001 | Front Biosci2001，6，： | 1 |
| 10 | Cripto-1 overexpres- sion leads to enhanced invasiveness and resistance to anoikis in hu- man MCF-7 breast cancer cells 显示文摘 | Normanno N De Luca A Bianco C | 2004 | J Cell Physiol2004，198，1： | 1 |
| 11 | Epidermal growth factor receptor(EGFR) signaling in cancer 显示文摘 | Normanno N De Luca A Bianco C | 2006 | Gene2006，366，1： | 1 |
| 12 | Target-based agents against ErbB receptors and their ligands: a novel approach to cancer treatment显示文摘 | Normanno N Bianco C De Luca A etal | 2003 | Endocr Relat Cancer2003，10，1： | 1 |
| 13 | Epidermal growth factor receptor (EGFR) signaling in cancer显示文摘 | Normanno N De Luca A Bianco C | 2006 | Gene2006，366，1： | 1 |
| 14 | Target -based agent against ErbB receptors and their ligands:A novel approach to cancer treatment显示文摘 | Normanno N Bianco C de Luca A | 2003 | Endocr Relat Cancer2003，10，： | 1 |
| 15 | Target-based agents against ErbB receptors and their ligands:a novel approach to cancer treatment显示文摘 | Normanno N Bianco C De Luca A | | 0，，： | 1 |
| 16 | Epidermal growth factor recep-tor (EGFR) signaling in cancer显示文摘 | Normanno N De Luca A Bianco C | 2006 | Gene2006，366，1： | 1 |
| 17 | Epidermal growth factor receptor (EGFR) signaling in cancer 显示文摘 | Normanno N De Luca A Bianco C | 2006 | Gene2006，366，1： | 1 |
| 18 | Epidermal growth factorreceptor ( EGFR ) signaling in cancer 显示文摘 | NORMANNO N DE LUCA A BIANCO C | 2006 | Gene2006，366，1： | 1 |
| 19 | Target-based agents against ErbB receptors and their ligands: a novel approach to cancer treatment显示文摘 | Normanno N Bianco C de Luca A | 2003 | Endocr Relat Cancer2003，10，1： | 1 |
| 20 | Dehydration of plant cells shoves nuclei rotation allowing for 3D phase-contrast tomography显示文摘Single-cell phase-contrast tomography promises to become decisive for studying 3D intracellular structures in biology.It involves probing cells with light at wide angles,which unfortunately requires complex systems.Here we show an intriguing concept based on an inherent natural process for plants biology,i.e.,dehydration,allowing us to easily obtain 3D-tomography of onion-epidermal cells’nuclei.In fact,the loss of water reduces the turgor pressure and we recognize it induces significant rotation of cells’nuclei.Thanks to the holographic focusing flexibility and an ad-hoc angles’tracking algorithm,we combine different phase-contrast views of the nuclei to retrieve their 3D refractive index distribution.Nucleolus identification capability and a strategy for measuring morphology,dry mass,biovolume,and refractive index statistics are reported and discussed.This new concept could revolutionize the investigation in plant biology by enabling dynamic 3D quantitative and label-free analysis at sub-nuclear level using a conventional holographic setup. | Zhe Wang Vittorio Bianco Daniele Pirone Pasquale Memmolo Massimiliano Maria Villone Pier Luca Maffettone Pietro Ferraro | 2021 | Light(Science & Applications)2021，10，10： | 1 |
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