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| 1 | Infantile hepatic hemangioendothelioma:A clinicopathologic study in a Chinese population显示文摘AIM:To investigate whether the clinicopathologic features of infantile hemangioendothelioma(IHE) of the liver in a Chinese population are similar to the features observed in other races.METHODS:The clinical data,radiological findings,histopathological changes and outcome of 12 cases of IHE diagnosed by the Department of Pathology,West China Hospital over the last 10 years were analyzed retrospectively.Immunohistochemical studies were carried out using antibodies against CD31,CD34,Factor Ⅷ,cytokeratin 8 and cytokeratin 18.RESULTS:The 12 patients were aged from fetal to 5 years(three males and nine females).The tumor was presented with different clinical manifestations,mainly as an asymptomatic,palpable,upper abdominal mass,except for the two fetuses who were detected antena-tally by ultrasound.In one patient,this presentation was accompanied by an initial severe pneumothorax.No symptoms of congestive heart failure were present and neither congenital abnormalities nor vascular tumors in the skin or other organs were found.Laboratory abnormalities included leukocytosis(40%),anemia(60%),thrombocytosis(60%),hyperbilirubinemia(16.7%),abnormal liver function(50%) and increased α-fetoprotein(80%).Based on radiological findings and gross specimens,the tumor presented as a solitary lesion or a multifocal space-occupying lesion.The tumor size ranged from 5.0 cm × 3.5 cm × 2.0 cm to 13.8 cm × 9.0 cm × 7.7 cm,and the 0.2-1.1 cm nodules were diffusely distributed within the multifocal tumor.Seven cases were surgically resected,three cases underwent biopsy and the two fetuses were aborted.Histologically,nine cases were classified as typeⅠ and three as type Ⅱ,presenting aggressive morphologic features,immature vessels,active mitosis and necrosis.An inflammatory component,predominantly eosinophilic granulocytes,sometimes obscured the nature of the tumor.Ten patients are alive after a follow-up of 1-9 years.Based on immunohistochemistry,the endothelial cells in all cases were positive for CD31,CD34 and polyclonal factor Ⅷ antigen,whereas the scattered hyperplasia bile ducts were positive for cytokeratin 8 and cytokeratin 18.CONCLUSION:The clinical manifestations of IHE are non-specific.There is no significant correlation between histological type and prognosis.The clinicopathologic features of IHE in Chinese patients may provide a clue to further evidence-based studies. | Zhang Zhang,Hui-Jiao Chen,Wen-Juan Yang,Hong Bu,Bing Wei,Xiao-Yu Long,Jing Fu,Rui Zhang,Yun-Bi Ni,Hong-Ying Zhang,Department of Pathology,West China Hospital,Sichuan University,Guoxuexiang 37,Chengdu 610041,Sichuan Province,China | 2010 | World Journal of Gastroenterology2010，16，36： | 16 |
| 2 | Xiaotan Sanjie decoction attenuates tumor angiogenesis by manipulating Notch-1-regulated proliferation of gastric cancer stem-like cells显示文摘AIM: To determine the underlying mechanisms of action and influence of Xiaotan Sanjie(XTSJ) decoction on gastric cancer stem-like cells(GCSCs). METHODS: The gastric cancer cell line MKN-45 line was selected and sorted by FACS using the cancer stem cell marker CD44; the stemness of these cells was checked in our previous study. In an in vitro study, the expression of Notch-1, Hes1, Vascular endothelial growth factor(VEGF), and Ki-67 in both CD44-positive gastric cancer stem-like cells(GCSCs) and CD44-negative cells was measured by Western blot. The effect of XTSJ serum on cell viability and on the above markers was measured by MTT assay and Western blot, respectively. In an in vivo study, the ability to induce angiogenesis and maintenance of GCSCs in CD44-positive-MKN-45- and CD44-negative-engrafted mice were detected by immunohistochemical staining using markers for CD34 and CD44, respectively. The role of XTSJ decoction in regulating the expression of Notch-1, Hes1, VEGF and Ki-67 was measured by Western blot and real-time polymerase chain reaction.RESULTS: CD44+ GCSCs showed more cell proliferation and VEGF secretion than CD44-negative cells in vitro, which were accompanied by the high expression of Notch-1 and Hes1 and positively associated with tumor growth(GCSCs vs CD44-negative cells, 2.72 ± 0.25 vs 1.46 ± 0.16, P < 0.05) and microvessel density(MVD)(GCSCs vs CD44-negative cells, 8.15 ± 0.42 vs 3.83 ± 0.49, P < 0.001) in vivo. XTSJ decoction inhibited the viability of both cell types in a dose-dependent manner in vitro. Specifically, a significant difference in the medium-(82.87% ± 6.53%) and high-dose XTSJ groups(77.43% ± 7.34%) was detected at 24 h in the CD44+ GCSCs group compared with the saline group(95.42% ± 5.76%) and the low-dose XTSJ group(90.74% ± 6.57%)(P < 0.05). However, the efficacy of XTSJ decoction was reduced in the CD44- groups; significant differences were only detected in the high-dose XTSJ group at 48 h(78.57% ± 6.94%) and 72 h(72.12% ± 7.68%) when compared with the other CD44- groups(P < 0.05). Notably, these differences were highly consistent with the Notch-1, Hes1, VEGF and Ki-67 expression in these cells. Similarly, in vivo, XTSJ decoction inhibited tumor growth in a dose-dependent manner. A significant difference was observed in the medium(1.76 ± 0.15) and high-dose XTSJ(1.33 ± 0.081) groups compared with the GCSCs control group(2.72 ± 0.25) and the low-dose XTSJ group(2.51 ± 0.25)(P < 0.05). We also detected a remarkable decrease of MVD in the medium-(7.10 ± 0.60) and high-dose XTSJ(5.99 ± 0.47) groups compared with the GCSC control group(8.15 ± 0.42) and the low-dose XTSJ group(8.14 ± 0.46)(P < 0.05). Additionally, CD44 expression was decreased in these groups [medium-(4.43 ± 0.45) and high-dose XTSJ groups(3.56 ± 0.31) vs the GCSC control(5.96 ± 0.46) and low dose XTSJ groups(5.91 ± 0.38)](P < 0.05). The significant differences in Notch-1, Hes1, VEGF and Ki-67 expression highly mirrored the results of XTSJ decoction in inhibiting tumor growth, MVD and CD44 expression.CONCLUSION: Notch-1 may play an important role in regulating the proliferation of GCSCs; XTSJ decoction could attenuate tumor angiogenesis, at least partially, by inhibiting Notch-1. | Bing Yan Long Liu Ying Zhao Li-Juan Xiu Da-Zhi Sun Xuan Liu Ye Lu Jun Shi Yin-Cheng Zhang Yong-Jin Li Xiao-Wei Wang Yu-Qi Zhou Shou-Han Feng Can Lv Pin-Kang Wei Zhi-Feng Qin | 2014 | World Journal of Gastroenterology2014，20，36： | 14 |
| 3 | MicroRNA-191 targets N-deacetylase/N-sulfotransferase 1 and promotes cell growth in human gastric carcinoma cell line MGC803显示文摘作为基因表示的 post-transcriptional 管理者的一个家庭, microRNAs (miRNAs ) 控制包括房间区别,增长和 apoptosis 的生物进程的一个宽数组,和 miRNAs 的 dysregulation 是癌症的一个特点。这里,我们发现 microRNA-191 (miR-191 ) 在在人的胃的腺癌房间线 MGC803 和人的胃的癌症纸巾的高表示的水平。-2,5-diphenyltetrazolium 溴化物和殖民地形成试金显示出的 3-(4,5-dimethylthiazol-2-yl ) 的结果那 miR-191 能支持房间生长并且压制 MGC803 房间的 apoptosis。N-deacetylase/N-sulfotransferase 1 (NDST1 ) 被证实是提高的绿色边的 miR-191 的直接目标基因荧光灯蛋白质记者实验。NDST1 的 mRNA 和蛋白质层次相反地在 MGC803 房间与 miR-191 被相关,建议由 miR-191 的 NDST1 的否定规定。而且, NDST1 起了一个禁止的作用并且能压制 MGC803 房间增长。我们的调查结果建议 miR-191 能在 MGC803 房间充当 oncogene,并且细胞的功能部分由于它 NDST1 的否定规定。 | Xuejun Shi Shicang SU Jian Long Bing Mei Yajun Chen | 2011 | Acta Biochimica et Biophysica Sinica2011，43，11： | 12 |
| 4 | Fixation of CO_2 by electrocatalytic reduction to synthesis of dimethyl carbonate in ionic liquid using effective silver-coated nanoporous copper composites显示文摘与高表面区域,开的孔和高效率,催化剂在 electrocatalytic 被准备并且第一采用了 CO2 的减小和 dimethyl 碳酸盐(直接数字控制) 的 electrosynthesis。为在离子的液体的 CO2 的 electrocatalytic 减小的电气化学的性质被周期的 voltammogram (CV ) 学习。象 electrocatalytic 性能上的温度,工作潜力和阴极材料一样的各种各样的反应变量的效果也被调查。直接数字控制的 80% 产量在最佳的反应条件下面被获得。 | Xuan Yun Wang Su Qin Liu Ke Long Huang Qiu Ju Feng De Lai Ye Bing Liu Jin Long Liu Guan Hua Jin | 2010 | Chinese Chemical Letters2010，21，8： | 10 |
| 5 | The development of MOFs-based nanomaterials in heterogeneous organocatalysis显示文摘Metal-organic framework(MOF) is a class of inorganic-organic hybrid material assembled periodically with metal ions and organic ligands. MOFs have always been the focuses in a variety of frontier fields owing to the advantageous properties, such as large BET surface areas, tunable porosity and easyfunctionalized surface structure. Among the various application areas, catalysis is one of the earliest application fields of MOFs-based materials and is one of the fastest-growing topics. In this review, the main roles of MOFs in heterogeneous organocatalysis have been systematically summarized, including used as support materials(or hosts), independent catalysts, and sacrificial templates. Moreover, the application prospects of MOFs in photocatalysis and electrocatalysis frontiers were also mentioned.Finally, the key issues that should be conquered in future were briefly sketched in the final parts of each item. We hope our perspectives could be beneficial for the readers to better understand these topics and issues, and could also provide a direction for the future exploration of some novel types of MOFs-based nanocatalysts with stable structures and functions for heterogeneous catalysis. | Xiaomei Liu Bing Tang Jilan Long Wei Zhang Xiaohong Liu Zakaria Mirza | 2018 | Science Bulletin2018，63，8： | 10 |
| 6 | The long rather than the short allele of 5-HTTLPR predisposes Han Chinese to anxiety and reduced connectivity between prefrontal cortex and amygdala显示文摘The short allele of the serotonin-transporter gene is associated with higher risk for anxiety and depression in Caucasians, but this association is still unclear in Asians. Here, we addressed this issue using behavioral and multi-modal MRI approaches in a large group of healthy Han Chinese participants (n = 233). In contrast to findings in Caucasians, we found that long-allele (L) carriers had higher anxiety scores. In another group (n = 64) experiencing significant levels of depression or anxiety, the L-allele frequency was also significantly higher. In healthy participants, L-carriers had reduced functional and anatomical connectivity between the amygdala and prefrontal cortex (PFC), which was correlated with anxiety or depression scores. Our findings demonstrated that in Chinese Han participants, in contrast to Caucasians, the L-allele confers vulnerability to anxiety or depression and weakens top-down emotional control between the PFC and amygdala. Therefore, ethnic background should be taken into account in gene-related studies and their potential clinical applications. | Haixia Long Bing Liu Bing Hou Chao Wang Jin Li Wen Qin Dawei Wang Yuan Zhou Keith M. Kendrick Chunshui Yu Tianzi Jiang | 2013 | Neuroscience Bulletin2013，29，1： | 9 |
| 7 | High glucose causes apoptosis of rabbit corneal epithelial cells involving activation of PERK-eIF2α-CHOP-caspase-12 signaling pathway显示文摘AIM: To investigate the effect of high concentration of glucose(HCG) on double stranded RNA-activated protein kinase-like ER kinase(PERK)-eukaryotic initiation factor-2α(eIF2α)-transcription factor C/EBP homologous protein(CHOP)-cysteine aspartate specific proteinase(caspase-12) signaling pathway activation and apoptosis in rabbit corneal epithelial cells(RCECs). METHODS: RCECs were treated by different concentrations of glucose for 0-48 h. The expressions of PERK, p-PERK, eIF2α, p-eIF2α, 78 k Da glucose-regulated protein 78(GRP78), CHOP, B-cell lymphoma 2(Bcl-2), B-cell lymphoma-2-associated X protein(Bax) and caspase-12 were determined by Western blot. Apoptosis was detected by TUNEL assay. Meanwhile, the function of PERK-eI F2α-CHOP-caspase-12 signaling pathway activation in high glucose-induced apoptosis was evaluated using PERK inhibitor, GSK2606414. RESULTS: HCG significantly promoted the expression of p-PERK, p-eIF2α, GRP78, CHOP, Bax and cleaved caspase-12 in RCECs(P<0.05), while remarkably decreased the expression of Bcl-2 and caspase-12(P<0.05), and the alterations caused by glucose were in concentration-and time-dependent manners. Meanwhile, PERK and eIF2α expressions were not affected in all groups(P>0.05). TUNEL assay showed that the apoptosis rate of RCECs in the HCG group increased significantly in contrast with that in the normal concentration of glucose or osmotic pressure control group(P<0.05), and the apoptosis rate increased with the increase of glucose concentration within limits(P<0.05). GSK2606414 down-regulated the expression of p-PERK and p-eI F2α in the HCG group(P<0.05), while still did not affect the expression of PERK and eIF2α among groups(P>0.05). Correspondingly, GSK2606414 also significantly reduced the apoptosis rate induced by high glucose(P<0.05). CONCLUSION: HCG activates PERK-eIF2α-CHOPcaspase-12 signaling pathway and promotes apoptosis of RCECs. | Pan-Pan Yao Min-Jie Sheng Wen-Hao Weng Yin Long Hao Liu Li Chen Jia-Jun Lu Ao Rong Bing Li | 2019 | International Journal of Ophthalmology(English edition)2019，12，12： | 7 |
| 8 | Percutaneous extraction of leads from coronary sinus vein and branch by modified techniques显示文摘 | CHU Xian-ming LI Xue-bin ZHANG Ping, WANG Long LI Ding LI Bing AN Yi LENG Min DUAN Jiang-bo GUO Ji-hong | 2012 | Chinese Medical Journal2012，，20： | 6 |
| 9 | Antigens synthesis and antibodies preparation for furazolidone and its metabolite 3-amino-2-oxazolidinone显示文摘3- 的二 haptens [(5-amino-furan-2-ylmethylene ) 氨基] oxazolidin-5-one (FZ-NH2 ) 和 3-{[(4-carboxyphenyl ) 甲又]-amino}-2-oxazolidinone (CPAOZ ) 被综合。为 FZ-NH2, immunogens 被 glutaraldehyde 和重氮基的盐方法准备。为 CPAOZ, immunogens 被活跃酉旨的方法连接并且混合了酸酐。与联合相比,间接竞争连接酶的 immunosorbent 试金(ic-ELISA ) 经由活跃酉旨方法经由 glutaraldehyde 方法和 CPAOZ-KLH 的 immunogen 与 FZ-NH2-OVA 的涂层抗原被开发。为 furazolidone 和它的代谢物 AOZ (是的 NPAOZ 衍生物) ,敏感(IC50 ) 是 2.0 渭 g /L 和 2.5 渭 g /L,察觉(IC15 ) 的限制是 0.09 渭 g /L 和 0.25 渭 g /L 分别地。一个敏感方法为 furazolidone 的同时的决心被开发在喂并且它在织物的代谢物 AOZ。 | Hua Ping Zhu Ting Ting Liu Bing Liu Hui Ling Yin Xiao Long Li Li Wang Shuo Wang | 2010 | Chinese Chemical Letters2010，21，9： | 6 |
| 10 | MiR-125a-5p Regulates Vitamin D Receptor Expression in a Mouse Model of Experimental Autoimmune Encephalomyelitis显示文摘Multiple sclerosis(MS)is a chronic and incurable autoimmune neurodegenerative disease of the central nervous system.Although the symptoms of MS can be managed by vitamin D3 treatment alone,this condition cannot be completely eradicated.Thus,there might be unknown factors capable of regulating the vitamin D receptor(VDR).Genome-wide analysis showed that miRNAs were associated with VDRs.We sought to determine the role and mechanism of action of miRNA-125a-5p and VDRs in a model of MS,mice with experimental autoimmune encephalomyelitis(EAE),which was induced by myelin oligodendrocyte glycoprotein 35–55 peptides.EAE mice showed decreased mean body weight but increased mean clinical scores compared with vehicle or control mice.And inflammatory infiltration was found in the lumbosacral spinal cord of EAE mice.In addition,VDR expression was significantly lower while the expression of miR-125a-5p was markedly higher in the spinal ventral horn of EAE mice than in vehicle or control mice.Importantly,activation of VDRs by paricalcitol or inhibition of miR-125a-5p by its antagomir markedly decreased the mean clinical scores in EAE mice.Interestingly,VDR and miR-125a-5p were co-localized in the same neurons of the ventral horn.More importantly,inhibition of miR-125a-5p remarkably blocked the decrease of VDRs in EAE mice.These results support a critical role for miR-125a-5p in modulating VDR activity in EAE and suggest potential novel therapeutic interventions. | Han-Chun Long Rui Wu Chun-Feng Liu Fei-Long Xiong Zu Xu Dian He Yi-Fan Zhang Bing Shao Ping-An Zhang Guang-Yin Xu Lan Chu | 2020 | Neuroscience Bulletin2020，36，2： | 6 |
| 11 | 2020 roadmap on pore materials for energy and environmental applications显示文摘Porous materials have attracted great attention in energy and environment applications,such as metal organic frameworks(MOFs),metal aerogels,carbon aerogels,porous metal oxides.These materials could be also hybridized with other materials into functional composites with superior properties.The high specific area of porous materials offer them the advantage as hosts to conduct catalytic and electrochemical reactions.On one hand,catalytic reactions include photocatalytic,p ho toe lectrocatalytic and electrocatalytic reactions over some gases.On the other hand,they can be used as electrodes in various batteries,such as alkaline metal ion batteries and electrochemical capacitors.So far,both catalysis and batteries are extremely attractive topics.There are also many obstacles to overcome in the exploration of these porous materials.The research related to porous materials for energy and environment applications is at extremely active stage,and this has motivated us to contribute with a roadmap on ’porous materials for energy and environment applications’. | Zengxi Wei Bing Ding Hui Dou Jorge Gascon Xiang-Jian Kong Yujie Xiong Bin Cai Ruiyang Zhang Ying Zhou Mingce Long Jie Miao Yuhai Dou Ding Yuan Jianmin Ma | 2019 | Chinese Chemical Letters2019，30，12： | 5 |
| 12 | Consensus on the digestive endoscopic tunnel technique显示文摘With the digestive endoscopic tunnel technique(DETT), many diseases that previously would have been treated by surgery are now endoscopically curable by establishing a submucosal tunnel between the mucosa and muscularis propria(MP). Through the tunnel, endoscopic diagnosis or treatment is performed for lesions in the mucosa, in the MP, and even outside the gastrointestinal(GI) tract.At present, the tunnel technique application range covers the following:(1)Treatment of lesions originating from the mucosal layer, e.g., endoscopic submucosal tunnel dissection for oesophageal large or circular early-stage cancer or precancerosis;(2) treatment of lesions from the MP layer, per-oral endoscopic myotomy, submucosal tunnelling endoscopic resection, etc.; and(3) diagnosis and treatment of lesions outside the GI tract, such as resection of lymph nodes and benign tumour excision in the mediastinum or abdominal cavity. With the increasing number of DETTs performed worldwide, endoscopic tunnel therapeutics, which is based on DETT, has been gradually developed and optimized. However, there is not yet an expert consensus on DETT to regulate its indications, contraindications, surgical procedure, and postoperative treatment.The International DETT Alliance signed up this consensus to standardize the procedures of DETT. In this consensus, we describe the definition, mechanism,and significance of DETT, prevention of infection and concepts of DETTassociated complications, methods to establish a submucosal tunnel, and application of DETT for lesions in the mucosa, in the MP and outside the GI tract(indications and contraindications, procedures, pre-and postoperative treatments, effectiveness, complications and treatments, and a comparison between DETT and other operations). | Ning-Li Chai Hui-Kai Li En-Qiang Linghu Zhao-Shen Li Shu-Tian Zhang Yu Bao Wei-Gang Chen Philip WY Chiu Tong Dang Wei Gong Shu-Tang Han Jian-Yu Hao Shui-Xiang He Bing Hu1 Bing Hu2 Xiao-Jun Huang Yong-Hui Huang Zhen-Dong Jin Mouen A Khashab James Lau Peng Li Rui Li De-Liang Liu Hai-Feng Liu Jun Liu Xiao-Gang Liu Zhi-Guo Liu Ying-Cai Ma Gui-Yong Peng Long Rong Wei-Hong Sha Pateek Sharma Jian-Qiu Sheng Shui-Sheng Shi Dong Wan Seo Si-Yu Sun Gui-Qi Wang Wen Wang Qi Wu Hong Xu Mei-Dong Xu Ai-Ming Yang Fang Yao Hong-Gang Yu Ping-Hong Zhou Bin Zhang Xiao-Feng Zhang Ya-Qi Zhai | 2019 | World Journal of Gastroenterology2019，25，7： | 5 |
| 13 | Implementing a “free” tuberculosis (TB) care policy under the integrated model in Jiangsu, China: practices and costs in the real world显示文摘Background:In the 1990s,China introduced a“free”tuberculosis(TB)care policy under the national TB control program.Recently,as a part of a new TB diagnosis and treatment model,it has been recommended that the integrated model scale up.This paper examines whether or not TB designated hospitals in the selected project sites have provided TB care according to the national and local guidelines,and analyzes the actual practices and expenditures involved in completing TB treatment.It also explores the reasons why“free”TB care in China cannot be effectively implemented under the integrated model.Methods:This study was conducted in three counties of Zhenjiang city,Jiangsu province.Mixed methods were used,which comprised reviewing the national and local TB control guidelines,conducting TB patient surveys,collecting TB inpatient and outpatient hospital records,and conducting qualitative interviews with stakeholders.Descriptive statistics were used for quantitative data analysis across counties and in order to compare patients who received only outpatient care and those who received both outpatient and inpatient care.The chi-square test and analysis of variance were performed where necessary.Qualitative data were analyzed using the framework approach.Results:Although the national TB care guidelines recommend outpatient care as a basis for TB treatment in China,we found high hospital admission rates for TB patients ranging from 39%in Yangzhong county to 83%in Dantu county.Almost all outpatient TB patients paid for lab tests and over 80%paid for liver protection drugs and around 70%paid for image examinations.These three components accounted for three-quarters of the total outpatient expenditure.For patients who received only outpatient care,the total expenditure upon completion of TB treatment was on average 1,135 Chinese yuan.For patients who received outpatient and inpatient care,the total expenditure upon completion of TB treatment was 11,117 Chinese yuan.Conclusion:The“free”TB care policy under the integrated model has not been effectively implemented in China.There has been substantial spending on non-recommended services,examinations,and drugs for TB treatment. | Xinxin Jia Jiaying Chen Siyuan Zhang Bing Dai Qian Long Shenglan Tang | 2016 | Infectious Diseases of Poverty2016，5，1： | 4 |
| 14 | Application of Rollout Strategy to Test Points Selection for Integer-Coded Fault Wise Table显示文摘Test points selection for integer-coded fault wise table is a discrete optimization problem. The global minimum set of test points can only be guaranteed by an exhaustive search which is computationally expensive. In this paper, this problem is formulated as a heuristic depth-first graph search problem at first. The graph node expanding method and rules are given. Then, rollout strategies are applied, which can be combined with the heuristic graph search algorithms, in a computationally more efficient manner than the optimal strategies, to obtain solutions superior to those using the greedy heuristic algorithms. The proposed rollout-based test points selection algorithm is illustrated and tested using an analog circuit and a set of simulated integer-coded fault wise tables. Computational results are shown, which suggest that the rollout strategy policies are significantly better than other strategies. | Cheng-Lin Yang Shu-Lin Tian Bing Long | 2009 | Journal of Electronic Science and Technology of China2009，7，4： | 4 |
| 15 | Structural basis for bivalent binding and inhibition of SARS-CoV-2 infection by human potent neutralizing antibodies显示文摘Neutralizing monoclonal antibodies(nAbs)to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)represent promising candidates for clinical intervention against coronavirus disease 2019(COVID-19).We isolated a large number of nAbs from SARS-CoV-2-infected individuals capable of disrupting proper interaction between the receptor binding domain(RBD)of the viral spike(S)protein and the receptor angiotensin converting enzyme 2(ACE2).However,the structural basis for their potent neutralizing activity remains unclear.Here,we report cryo-EM structures of the ten most potent nAbs in their native full-length IgG-form or in both IgG-form and Fab-form bound to the trimeric S protein of SARS-CoV-2.The bivalent binding of the full-length IgG is found to associate with more RBDs in the“up”conformation than the monovalent binding of Fab,perhaps contributing to the enhanced neutralizing activity of IgG and triggering more shedding of the S1 subunit from the S protein.Comparison of a large number of nAbs identified common and unique structural features associated with their potent neutralizing activities.This work provides a structural basis for further understanding the mechanism of nAbs,especially through revealing the bivalent binding and its correlation with more potent neutralization and the shedding of S1 subunit. | Renhong Yan Ruoke Wang Bin Ju Jinfang Yu Yuanyuan Zhang Nan Liu Jia Wang Qi Zhang Peng Chen Bing Zhou Yaning Li Yaping Shen Shuyuan Zhang Long Tian Yingying Guo Lu Xia Xinyue Zhong Lin Cheng Xiangyang Ge Juanjuan Zhao Hong-Wei Wang Xinquan Wang Zheng Zhang Linqi Zhang Qiang Zhou | 2021 | Cell Research2021，31，5： | 4 |
| 16 | Effect of montmorillonite on kinetics of polyurethane preparation reaction显示文摘prepolymerization 和 polyurethane/montmorillonite 的治好的反应动力学分别地与端基分析和 FTIR 被学习了。prepolymerization 和治好的反应跟随了第二顺序动力学,这被发现。但是在 montmorillonite 以后从 42.7 kJ/mol 增加到 56.5 kJ/mol 的 prepolymerization 的激活精力在治好反应的反应系统,和激活精力被增加从 64.4 kJ/mol 减少了到 17.5 kJ/mol。 | You Cao Yu Jiang Shu Lu Zhao Xiao Jun Cai Mei Long Hu Bing Liao | 2008 | Chinese Chemical Letters2008，19，1： | 4 |
| 17 | Cytokines predict virological response in chronic hepatitis B patients receiving peginterferon alfa-2a therapy显示文摘BACKGROUND Chronic hepatitis B virus infection remains a major global public health problem.Peginterferon-alpha-2a(PEG-IFN)has direct antiviral and immunoregulatory effects,and it has become one of the first choice drugs for the treatment of chronic hepatitis B(CHB).Cytokines play an important role in immunity,and they directly inhibit viral replication and indirectly determine the predominant pattern of the host immune response.AIM To determine the correlation between cytokine/chemokine expression levels and response to PEG-IFN treatment in patients with CHB.METHODS Forty-six kinds of cytokines were analyzed before PEG-IFN therapy and at 24 wk during therapy in 26 CHB patients.RESULTS The monokine induced by INF-γ(CXCL9)and serum interferon-inducible protein 10(IP-10)levels at baseline were higher in virological responders than in nonvirological responders(NRs)and decreased during treatment,whereas the NRs did not exhibit significant changes.The macrophage inflammatory protein 1d(MIP-1d)levels at baseline and during treatment were significantly higher in the virological responders than in the NRs,while thymus and activation-regulated chemokine(TARC)levels at baseline and during treatment were significantly lower in the virological responders than in the NRs.The CXCL9,IP-10,MIP-1d,and TARC baseline levels exhibited the expected effects for interferon treatment.The area under the receiver operating characteristic curve values of CXCL9,IP-10,MIP-1d,and TARC for predicting virological responses were 0.787,0.799,0.787,and 0.77(P=0.01,0.013,0.01,and 0.021),respectively.CONCLUSION We found that cytokine levels before and during treatment may represent potential biomarkers to select CHB patients who can respond to PEG-IFN.Therefore,cytokines can be used as an indicator of antiviral drug selection before CHB treatment. | Wen-Kang Fu Jie Cao Ning-Ning Mi Chong-Fei Huang Long Gao Jin-Duo Zhang Ping Yue Bing Bai Yan-Yan Lin Wen-Bo Meng | 2020 | World Journal of Clinical Cases2020，8，11： | 4 |
| 18 | Data mining analysis of Professor Liu Shangyi's prescription characteristics in clinical medicine for the treatment of cancer patients with stomachache显示文摘目的:分析国医大师刘尚义教授治疗肿瘤患者胃痛的临床用药特点.方法:收集2014年1月到2016年7月就诊于刘尚义教授国医堂门诊癌症见胃痛的患者资料,采用关联规则阿Apriori算法、复杂系统熵聚类等无监督数据挖掘方法,探讨刘尚义教授治疗肿瘤患者胃痛的临床用药特点.结果:对筛选出的120例处方进行分析,确定处方中药物使用频次,药物之间的关联规则,挖掘出4个核心组合和2个新方.治疗后的胃痛症状分级明显低于治疗前,差异具有统计学意义(P〈0.001).结论:刘教授治疗肿瘤患者胃痛以活血散结、祛湿止痛为主. | Wen-Qi Huang Zhu Yang Dong-Xin Tang Li Luo Feng-Xi Long Jing-Hui Wang Bing Yang | 2018 | Traditional Medicine Research2018，3，1： | 3 |
| 19 | Image-based tree modeling显示文摘 | Ping Tan Gang Zeng Jingdong Wang Sing Bing Kang Long Quan | 2007 | ACM Transactions on Graphics (TOG)2007，，3： | 3 |
| 20 | The Philadelphia chromosome in leukemogenesis显示文摘The truncated chromosome 22 that results from the reciprocal translocation t(9;22)(q34;q11) is known as the Phila?delphia chromosome(Ph) and is a hallmark of chronic myeloid leukemia(CML).In leukemia cells,Ph not only impairs the physiological signaling pathways but also disrupts genomic stability.This aberrant fusion gene encodes the breakpoint cluster region?proto?oncogene tyrosine?protein kinase(BCR?ABL1) oncogenic protein with persistently enhanced tyrosine kinase activity.The kinase activity is responsible for maintaining proliferation,inhibiting differentia?tion,and conferring resistance to cell death.During the progression of CML from the chronic phase to the accelerated phase and then to the blast phase,the expression patterns of different BCR?ABL1 transcripts vary.Each BCR?ABL1 transcript is present in a distinct leukemia phenotype,which predicts both response to therapy and clinical outcome.Besides CML,the Ph is found in acute lymphoblastic leukemia,acute myeloid leukemia,and mixed?phenotype acute leukemia.Here,we provide an overview of the clinical presentation and cellular biology of different phenotypes of Ph?positive leukemia and highlight key findings regarding leukemogenesis. | Zhi-Jie Kang Yu-Fei Liu Ling-Zhi Xu Zi-Jie Long Dan Huang Ya Yang Bing Liu Jiu-Xing Feng Yu-Jia Pan Jin-Song Yan Quentin Liu | 2016 | Chinese Journal of Cancer2016，35，6： | 3 |
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