|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2显示文摘 | Huize Pan Di Guan Xiaomeng Liu Jingyi Li Lixia Wang Jun Wu Junzhi Zhou Weizhou Zhang Ruotong Ren Weiqi Zhang Ying Li Jiping Yang Ying Hao Tingting Yuan Guohong Yuan Hu Wang Zhenyu Ju Zhiyong Mao Jian Li Jing Qu FuchouTang Guang-Hui Liu | 2016 | Cell Research2016，26，2： | 32 |
| 2 | The putative tumor suppressor C53 interacts with the human telomerase reverse transcriptase hTERT and regulates telomerase activity显示文摘Telomerase is a large ribonucleoprotein complex that contains a catalytic telomerase reverse transcriptase(TERT)and an RNA template.Telomerase activity is tightly controlled by TERT expression,which is regulated at both the transcriptional and post-translational levels.However,the detailed molecular mechanisms of telomerase regulation and function are not fully understood.To identify cofactors that contribute to telomerase regulation,we employed a yeast two-hybrid system to screen for hTERT-interacting proteins,using the hTERT T-motif as bait.We identify C53 as a novel hTERT interaction partner.We show that C53 interacts with hTERT both in vivo and in vitro.C53 depletion increases telomerase activity,and C53 overexpression inhibits telomerase activity in MCF7 cells.In addition,the C53 leucine zipper domain(amino acids 301–400)is required for interaction with hTERT.Deleting the leucine zipper domain eliminates C53 interaction with hTERT and abrogates its inhibitory effect on telomerase activity.Taken together,our results demonstrate that C53 is a novel hTERT-associated protein that negatively regulates telomerase activity. | Junzhi Zhou Peng Xi Qi Zhou Deqiang Ding Yusheng Cong | 2014 | Chinese Science Bulletin2014，59，19： | 4 |
| 3 | Design of 85–105GHz Wideband Traveling Wave PIN Diode Switches and Attenuators with Radial Stubs显示文摘Wideband traveling wave PIN Diode switches and attenuators are proposed. The switches and attenuators consist of three shunt PIN diodes, six radial stubs, bias circuit and DC return circuit. Wide bandwidth is achieved with six different radial stubs as reflective load of the diodes. The stubs work as short circuit to the diodes at different frequency points for broad bandwidth. For the Single pole single throw(SPST) switch, the lowest measured Insertion loss(IL) is 0.75 dB and lower than 2.0dB in 85–105GHz, and its isolation is better than 21.3dB.As a Voltage-controlled variable attenuator(VCA), typical attenuation range is 23 dB and attenuation fluctuation is lower than 5dB in 85–105GHz. | YAO Changfei ZHOU Ming LUO Yunsheng ZHANG Junzhi WEI Xiang XU Conghai | 2017 | Chinese Journal of Electronics2017，26，1： | 3 |
| 4 | A Survey of Underwater Multi-Robot Systems显示文摘As a cross-cutting field between ocean development and multi-robot system(MRS),the underwater multi-robot system(UMRS)has gained increasing attention from researchers and engineers in recent decades.In this paper,we present a comprehensive survey of cooperation issues,one of the key components of UMRS,from the perspective of the emergence of new functions.More specifically,we categorize the cooperation in terms of task-space,motion-space,measurement-space,as well as their combination.Further,we analyze the architecture of UMRS from three aspects,i.e.,the performance of the individual underwater robot,the new functions of underwater robots,and the technical approaches of MRS.To conclude,we have discussed related promising directions for future research.This survey provides valuable insight into the reasonable utilization of UMRS to attain diverse underwater tasks in complex ocean application scenarios. | Ziye Zhou Jincun Liu Junzhi Yu | 2022 | IEEE/CAA Journal of Automatica Sinica2022，9，1： | 3 |
| 5 | Genetic expression in progeny of transgenic plants obtained by using pollen-tube pathway (or delivery) method and approach to the transformation mechanism显示文摘The origin and the recent progress of pollen_tube pathway method (PTPM) is described. The results of transforming wheat during 1990 to 1994 are reported briefly. They are: (ⅰ) Transformation frequencies were 3%-6%, even reached 13.5%; (ⅱ) the genetic expression of four continuous generations obtained from single individuals of transgenic plants by self_fertilization was investigated. Plants with higher expression of foreign gene were found in every generation (from T1 to T4), but a phenomenon of gene silence, in which the genotype contained foreign gene but did not express, was also observed. In addition, from the angle of fertilization biology the transformation mechanism of PTPM (or PTDM) was analyzed and discussed. It was considered that the essential principle of this method was just like that of protoplast method. | Junzhi Zeng Youqiang Wu Dongjiang Wang Jian Zhang Zhengrong Ma Zhiyong Zhou | 1998 | Chinese Science Bulletin1998，43，10： | 3 |
| 6 | Immunogenicity and protective efficacy of a recombinant protein subunit vaccine and an inactivated vaccine against SARS-CoV-2 variants in non-human primates显示文摘Emerging SARS-CoV-2 variants and the gradually decreasing neutralizing antibodies over time post vaccination have led to an increase in incidents of breakthrough infection across the world.To investigate the potential protective effect of the recombinant protein subunit COVID-19 vaccine targeting receptor-binding domain(RBD)(PS-RBD)and whole inactivated virus particle vaccine(IV)against the variant strains,in this study,rhesus macaques were immunized with PS-RBD or IV vaccine,followed by a Beta variant(B.1.351)challenge.Although neutralizing activity against the Beta variant was reduced compared with that against the prototype,the decreased viral load in both upper and lower respiratory tracts,milder pathological changes,and downregulated inflammatory cytokine levels in lung tissues after challenge demonstrated that PS-RBD and IV still provided effective protection against the Beta variant in the macaque model.Furthermore,PS-RBD-induced macaque sera possessed general binding and neutralizing activity to Alpha,Beta,Delta,and Omicron variants in our study,though the neutralizing antibody(NAb)titers declined by varying degrees,demonstrating potential protection of PS-RBD against current circulating variants of concern(VOCs).Interestingly,although the IV vaccine-induced extremely low neutralizing antibody titers against the Beta variant,it still showed reduction for viral load and significantly alleviated pathological change.Other correlates of vaccine-induced protection(CoP)like antibody-dependent cellular cytotoxicity(ADCC)and immune memory were both confirmed to be existing in IV vaccinated group and possibly be involved in the protective mechanism. | Qian He Qunying Mao Xiaozhong Peng Zhanlong He Shuaiyao Lu Jialu Zhang Fan Gao Lianlian Bian Chaoqiang An Wenhai Yu Fengmei Yang Yanan Zhou Yun Yang Yanyan Li Yadi Yuan Xujia Yan Jinghuan Yang Xing Wu Weijin Huang Changgui Li Junzhi Wang Zhenglun Liang Miao Xu | 2022 | Signal Transduction and Targeted Therapy2022，7，4： | 2 |
| 7 | Ufl1 deficiency causes kidney atrophy associated with disruption of endoplasmic reticulum homeostasis显示文摘The UFMylation modification is a novel ubiquitin-like conjugation system,consisting of UBA5(E1),UFC1(E2),UFL1(E3),and the conjugating molecule UFM1.Deficiency in this modification leads to embryonic lethality in mice and diseases in humans.However,the function of UFL1 is poorly characterized.Studies on Ufl1 conditional knockout mice have demonstrated that the deletion of Ufl1 in cardiomyocytes and in intestinal epithelial cells causes heart failure and increases susceptibility to experimentally induced colitis,respectively,suggesting an essential role of UFL1 in the maintenance of the homeostasis in these organs.Yet,its physiological function in other tissues and organs remains completely unknown.In this study,we generate the nephron tubules specific Ufl1 knockout mice and find that the absence of Ufl1 in renal tubular results in kidney atrophy and interstitial fibrosis.In addition,Ufl1 deficiency causes the activation of unfolded protein response and cell apoptosis,which may be responsible for the kidney atrophy and interstitial fibrosis.Collectively,our results have demonstrated the crucial role of UFL1 in regulating kidney function and maintenance of endoplasmic reticulum homeostasis,providing another layer of understanding kidney atrophy. | You Zhou Xifu Ye Chenlu Zhang Jiabao Wang Zeyuan Guan Juzhen Yan Lu Xu Ke Wang Di Guan Qian Liang Jian Mao Junzhi Zhou Qian Zhang Xiaoying Wu Miao Wang Yu-Sheng Cong Jiang Liu | 2021 | Journal of Genetics and Genomics2021，48，5： | 1 |
| 8 | Development of novel-nanobody-based lateral-flow immunochromatographic strip test for rapid detection of recombinant human interferon a2b显示文摘Recombinant human interferon a2b(rhIFNa2b)is widely used as an antiviral therapy agent for the treatment of hepatitis B and hepatitis C.The current identification test for rhIFNa2b is complex.In this study,an anti-rhIFNa2b nanobody was discovered and used for the development of a rapid lateral flow strip for the identification of rhIFNa2b.RhIFNa2b was used to immunize an alpaca,which established a phage nanobody library.After five steps of enrichment,the nanobody I22,which specifically bound rhIFNa2b,was isolated and inserted into the prokaryotic expression vector pET28a.After subsequent purification,the physicochemical properties of the nanobody were determined.A semiquantitative detection and rapid identification assay of rhIFNa2b was developed using this novel nanobody.To develop a rapid test,the nanobody I22 was coupled with a colloidal gold to produce lateral-flow test strips.The developed rhIFNa2b detection assay had a limit of detection of 1 mg/mL.The isolation of I22 and successful construction of a lateral-flow immunochromatographic test strip demonstrated the feasibility of performing ligand-binding assays on a lateral-flow test strip using recombinant protein products.The principle of this novel assay is generally applicable for the rapid testing of other commercial products,with a great potential for routine use in detecting counterfeit recombinant protein products. | Xi Qin Maoqin Duan Dening Pei Jian Lin Lan Wang Peng Zhou Wenrong Yao Ying Guo Xiang Li Lei Tao Youxue Ding Lan Liu Yong Zhou Chuncui Jia Chunming Rao Junzhi Wang | 2022 | Journal of Pharmaceutical Analysis2022，12，2： | 0 |
| 9 | Free or fixed state of nHAP differentially regulates hBMSC morphology and osteogenesis through the valve role of ITGA7显示文摘Nano-hydroxyapatite(nHAP)has been widely used in bone repair as an osteo-inductive and naturally-occurring material.However,the optimal applied form of nHAP and the underlying mechanisms involved remain unclear.Herein,to investigate into these,a range of corresponding models were designed,including three applied forms of nHAP(Free,Coating and 3D)that belong to two states(Free or fixed).The results indicate that when fixed nHAP was applied in the 3D form,optimal osteogenesis was induced in human bone marrow stem cells(hBMSCs)with increased bone volume via integrinα7(ITGA7)-mediated upregulation of the PI3K-AKT signaling pathway,while contrary results were observed with free nHAP.Ectopic osteogenesis experiments in mice subcutaneous transplantation model further confirmed the different tendencies of ITGA7 expression and osteogenesis of hBMSCs in free and fixed states of nHAP.Our results revealed that the two states of nHAP play a different regulatory role in cell morphology and osteogenesis through the valve role of ITGA7,providing cues for better application of nanoparticles and a potential new molecular target in bone tissue engineering. | Fangyuan Bao Junzhi Yi Yixiao Liu Yuliang Zhong Hui Zhang Zhonglin Wu Boon Chin Heng Ying Wang Ziyang Wang Lizi Xiao Hua Liu Hongwei Ouyang Jing Zhou | 2022 | Bioactive Materials2022，7，12： | 0 |
| 10 | A survey of the pursuit-evasion problem in swarm intelligence显示文摘For complex functions to emerge in artificial systems,it is important to understand the intrinsic mechanisms of biological swarm behaviors in nature.In this paper,we present a comprehensive survey of pursuit–evasion,which is a critical problem in biological groups.First,we review the problem of pursuit–evasion from three different perspectives:game theory,control theory and artificial intelligence,and bio-inspired perspectives.Then we provide an overview of the research on pursuit–evasion problems in biological systems and artificial systems.We summarize predator pursuit behavior and prey evasion behavior as predator–prey behavior.Next,we analyze the application of pursuit–evasion in artificial systems from three perspectives,i.e.,strong pursuer group vs.weak evader group,weak pursuer group vs.strong evader group,and equal-ability group.Finally,relevant prospects for future pursuit–evasion challenges are discussed.This survey provides new insights into the design of multi-agent and multi-robot systems to complete complex hunting tasks in uncertain dynamic scenarios. | Zhenxin MU Jie PAN Ziye ZHOU Junzhi YU Lu CAO | 2023 | Frontiers of Information Technology & Electronic Engineering2023，24，8： | 0 |
| 11 | Bio-immobilization and recovery of chromium using a denitrifying biofilm system: Identification of reaction zone, binding forms and end products显示文摘Chromium is an important resource in strategic metals.Different from most studies focusing on the bio-reduction of hexavalent chromium[Cr(VI)],this study aims to achieve the immobilization and recovery of chromium using a sequencing batch biofilm reactor.Results showed that Cr(VI) removal efficiency remained more than 99%,and 97%of reduced Cr(III) was immobilized in the biofilm.Immobilization zone,chromium forms and extracellular polymeric substances composition changes were combined to reveal the mechanism of Cr(VI) reduction and immobilization.The chromium distribution in biofilm demonstrated that intercellular layer was the main active zone with an immobilization amount of 891.70±126.32 mg/g-VSS.The reduced products analysis confirmed that trivalent chromium[Cr(III)]chelated with carboxyl,amino and other functional groups and immobilized in the form of organic Cr(III).The digestion method realized a chromium recovery efficiency of 74.59%.This study provides an alternative method for the bioremediation and resources recovery in chromium polluted wastewater. | Yingxin Zhao Junzhi Gao Xu Zhou Zhouran Li Cailian Zhao Xulong Jia Min Ji | 2023 | Journal of Environmental Sciences2023，，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员