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| 1 | De novo characterization of the root transcriptome of a traditional Chinese medicinal plant Polygonum cuspidatum显示文摘Various active components have been extracted from the root of Polygonum cuspidatum.However,the genetic basis for their activity is virtually unknown.In this study,25600002 short reads (2.3 Gb) of P.cuspidatum root transcriptome were obtained via Illumina HiSeq 2000 sequencing.A total of 86418 unigenes were assembled de novo and annotated.Twelve,18,60 and 54 unigenes were respectively mapped to the mevalonic acid (MVA),methyl-D-erythritol 4-phosphate (MEP),shikimate and resveratrol biosynthesis pathways,suggesting that they are involved in the biosynthesis of pharmaceutically important anthraquinone and resveratrol.Eighteen potential UDP-glycosyltransferase unigenes were identified as the candidates most likely to be involved in the biosynthesis of glycosides of secondary metabolites.Identification of relevant genes could be important in eventually increasing the yields of the medicinally useful constituents of the P.cuspidatum root.From the previously published transcriptome data of 19 non-model plant taxa,1127 shared orthologs were identified and characterized.This information will be very useful for future functional,phylogenetic and evolutionary studies of these plants. | HAO DaCheng MA Pei MU Jun CHEN ShiLin XIAO PeiGen PENG Yong HUO Li XU LiJia SUN Chao | 2012 | Science China(Life Sciences)2012，55，5： | 36 |
| 2 | Anti-Helicobacter pylori therapy followed by celecoxib on progression of gastric precancerous lesions显示文摘AIM:To evaluate whether celecoxib,a selective cyclooxygenase 2(COX-2) inhibitor,could reduce the severity of gastric precancerous lesions following Helicobacter pylori(H pylori) eradication.METHODS:H pylori-eradicated patients with gastric precancerous lesions randomly received either celecoxib(n=30) or placebo(n=30) for up to 3 mo.COX-2 expression and activity was determined by immunostaining and prostaglandin E2(PGE2) assay,cell proliferation by Ki-67 immunostaining,apoptosis by TUNEL staining and angiogenesis by microvascular density(MVD) assay using CD31 staining.RESULTS:COX-2 protein expression was significantly increased in gastric precancerous lesions(atrophy,intestinal metaplasia and dysplasia,respectively) compared with chronic gastritis,and was concomitant with an increase in cell proliferation and angiogenesis.A significant improvement in precancerous lesions was observed in patients who received celecoxib compared with those who received placebo(P<0.001).Of these three changes,84.6% of sites with dysplasia regressed in patients treated with celecoxib(P=0.002) compared with 60% in the placebo group,suggesting that celecoxib was effective on the regression of dysplasia.COX-2 protein expression(P<0.001) and COX-2 activity(P<0.001) in the gastric tissues were consistently lower in celecoxib-treated patients compared with the placebo-treated subjects.Moreover,it was also shown that celecoxib suppressed cell proliferation(P<0.01),induced cell apoptosis(P<0.01) and inhibited angiogenesis with decreased MVD(P<0.001).However,all of these effects were not seen in placebo-treated subjects.Furthermore,COX-2 inhibition resulted in the up-regulation of PPARγ expression,a protective molecule with anti-neoplastic effects.CONCLUSION:H pylori eradication therapy followed by celecoxib treatment improves gastric precancerous lesions by inhibiting COX-2 activity,inducing apoptosis,and suppressing cell proliferation and angiogenesis. | Li-Jing Zhang Shi-Yan Wang Xiao-Hui Huo Zhen-Long Zhu Jian-Kun Chu Jin-Cheng Ma Dong-Sheng Cui Ping Gu Zeng-Ren Zhao Ming-Wei Wang Jun Yu | 2009 | World Journal of Gastroenterology2009，15，22： | 32 |
| 3 | Chinese Trauma Surgeon Association for management guidelines of vacuum sealing drainage application in abdominal surgeries-Update and systematic review显示文摘Vacuum sealing drainage (VSD) is frequently used in abdominal surgeries. However, relevant guidelines are rare. Chin ese Trauma Surge on Associati on orga nized a committee composed of 28 experts across China in July 2017, aiming to provide an evidence-based recommendation for the application of VSD in abdominal surgeries.Eleven questions regarding the use of VSD in abdominal surgeries were addressed:(1) which type of materials should be respectively chosen for the intraperitoneal cavity, retroperitoneal cavity and superficial incisions?(2) Can VSD be preventively used for a high-risk abdominal incision w让h primary suture?(3) Can VSD be used in severely contaminated/infected abdominal surgical sites?(4) Can VSD be used for temporary abdominal cavity closure under some special conditions such as severe abdominal trauma, infection, liver transplantation and intra-abdominal volume increment in abdominal compartment syndrome?(5) Can VSD be used in abdominal organ inflammation, injury, or postoperative drainage?(6) Can VSD be used in the treatment of intestinal fistula and pancreatic fistula?(7) Can VSD be used in the treatment of intra-abdominal and extra-peritoneal abscess?(8) Can VSD be used in the treatment of abdominal wall wounds, wound cavity, and defects?(9) Does VSD in crease the risk of bleeding?(10) Does VSD increase the risk of intestinal wail injury?(11) Does VSD increase the risk of peritoneal adhesion? Focusing on these questions, evidence-based recommendations were given accordingly. VSD was strongly recommended regarding the questions 2-4. Weak recommendations were made regarding questions 1 and 5-11. Proper use of VSD in abdominal surgeries can lower the risk of infection in abdominal incisions with primary suture, treat severely contaminated/infected surgical sites and facilitate temporary abdominal cavity closure. | Yang Li Pei-Yuan Li Shi-Jing Sun Yuan-Zhang Yao Zhan-Fei Li Tao Liu Fan Yang Lian-Yang Zhang Xiang-Jun Bai Jing-Shan Huo Wu-Bing He Jun Ouyang Lei Peng Ping Hu Yan-An Zhu Ping Jin Qi-Feng Shao Yan-Feng Wang Rui-Wu Dai Pei-Yang Hu Hai-Ming Chen Ge-Fei Wang Yong-Gao Wang Hong-Xu Jin Chang-Ju Zhu Qi-Yong Zhang Biao Shao Xi-Guang Sang Chang-Lin Yin | 2019 | Chinese Journal of Traumatology2019，22，1： | 26 |
| 4 | Microtubule-associated deacetylase HDAC6 promotes angiogenesis by regulating cell migration in an EB1-dependent manner显示文摘Angiogenesis,a process by which the preexisting blood vasculature gives rise to new capillary vessels,is associated with a variety of physiologic and pathologic conditions.However,the molecular mechanism underlying this important process remains poorly understood.Here we show that histone deacetylase 6(HDAC6),a microtubule-associated enzyme critical for cell motility,contributes to angiogenesis by regulating the polarization and migration of vascular endothelial cells.Inhibition of HDAC6 activity impairs the formation of new blood vessels in chick embryos and in angioreactors implanted in mice.The requirement for HDAC6 in angiogenesis is corroborated in vitro by analysis of endothelial tube formation and capillary sprouting.Our data further show that HDAC6 stimulates membrane ruffling at the leading edge to promote cell polarization.In addition,microtubule end binding protein 1(EB1)is important for HDAC6 to exert its activity towards the migration of endothelial cells and generation of capillary-like structures.These results thus identify HDAC6 as a novel player in the angiogenic process and offer novel insights into the molecular mechanism governing endothelial cell migration and angiogenesis. | Dengwen Li Songbo Xie Yuan Ren Lihong Huo Jinmin Gao Dandan Cui Min Liu Jun Zhou | 2011 | Protein & Cell2011，2，2： | 13 |
| 5 | Meningeal lymphatic vessels regulate brain tumor drainage and immunity显示文摘Recent studies have shown that meningeal lymphatic vessels(MLVs),which are located both dorsally and basally beneath the skull,provide a route for draining macromolecules and trafficking immune cells from the central nervous system(CNS)into cervical lymph nodes(CLNs),and thus represent a potential therapeutic target for treating neurodegenerative and neuroinflammatory diseases.However,the roles of MLVs in brain tumor drainage and immunity remain unexplored.Here we show that dorsal MLVs undergo extensive remodeling in mice with intracranial gliomas or metastatic melanomas.RNA-seq analysis of MLV endothelial cells revealed changes in the gene sets involved in lymphatic remodeling,fluid drainage,as well as inflammatory and immunological responses.Disruption of dorsal MLVs alone impaired intratumor fluid drainage and the dissemination of brain tumor cells to deep CLNs(dCLNs).Notably,the dendritic cell(DC)trafficking from intracranial tumor tissues to dCLNs decreased in mice with defective dorsal MLVs,and increased in mice with enhanced dorsal meningeal lymphangiogenesis.Strikingly,disruption of dorsal MLVs alone,without affecting basal MLVs or nasal LVs,significantly reduced the efficacy of combined anti-PD-1/CTLA-4 checkpoint therapy in striatal tumor models.Furthermore,mice bearing tumors overexpressing VEGF-C displayed a better response to anti-PD-1/CTLA-4 combination therapy,and this was abolished by CCL21/CCR7 blockade,suggesting that VEGF-C potentiates checkpoint therapy via the CCL21/CCR7 pathway.Together,the results of our study not only demonstrate the functional aspects of MLVs as classic lymphatic vasculature,but also highlight that they are essential in generating an efficient immune response against brain tumors. | Xueting Hu Qiuping Deng Lu Ma Qingqing Li Yidong Chen Yuhan Liao Fan Zhou Chen Zhang Linlin Shao Jun Feng Tubao He Weihai Ning Yan Kong Yingqing Huo Aibin He Bing Liu Jingjing Zhang Ralf Adams Yulong He Fuchou Tang Xiuwu Bian Jincai Luo | 2020 | Cell Research2020，30，3： | 11 |
| 6 | Immunity-and-matrix-regulatory cells derived from human embryonic stem cells safely and effectively treat mouse lung injury and fibrosis显示文摘Lung injury and fibrosis represent the most significant outcomes of severe and acute lung disorders,including COVID-19.However,there are still no effective drugs to treat lung injury and fibrosis.In this study,we report the generation of clinical-grade human embryonic stem cells(hESCs)-derived immunity-and matrix-regulatory cells(IMRCs)produced under good manufacturing practice requirements,that can treat lung injury and fibrosis in vivo.We generate IMRCs by sequentially differentiating hESCs with serum-free reagents.IMRCs possess a unique gene expression profile distinct from that of umbilical cord mesenchymal stem cells(UCMSCs),such as higher expression levels of proliferative,immunomodulatory and anti-fibrotic genes.Moreover,intravenous delivery of IMRCs inhibits both pulmonary inflammation and fibrosis in mouse models of lung injury,and significantly improves the survival rate of the recipient mice in a dose-dependent manner,likely through paracrine regulatory mechanisms.IMRCs are superior to both primary UCMSCs and the FDA-approved drug pirfenidone,with an excellent efficacy and safety profile in mice and monkeys.In light of public health crises involving pneumonia,acute lung injury and acute respiratory distress syndrome,our findings suggest that IMRCs are ready for clinical trials on lung disorders. | Jun Wu Dingyun Song Zhongwen Li Baojie Guo Yani Xiao Wenjing Liu Lingmin Liang Chunjing Feng Tingting Gao Yanxia Chen Ying Li Zai Wang Jianyan Wen Shengnan Yang Peipei Liu Lei Wang Yukai Wang Liang Peng Glyn Nigel Stacey Zheng Hu Guihai Feng Wei Li Yan Huo Ronghua Jin Ng Shyh-Chang Qi Zhou Liu Wang Baoyang Hu Huaping Dai Jie Hao | 2020 | Cell Research2020，30，9： | 7 |
| 7 | CD147 antibody specifically and effectively inhibits infection and cytokine storm of SARS-CoV-2 and its variants delta,alpha,beta,and gamma显示文摘SARS-CoV-2 mutations contribute to increased viral transmissibility and immune escape,compromising the effectiveness of existing vaccines and neutralizing antibodies.An in-depth investigation on COVID-19 pathogenesis is urgently needed to develop a strategy against SARS-CoV-2 variants.Here,we identified CD147 as a universal receptor for SARS-CoV-2 and its variants.Meanwhile,Meplazeumab,a humanized anti-CD147 antibody,could block cellular entry of SARS-CoV-2 and its variants-alpha,beta,gamma,and delta,with inhibition rates of 68.7,75.7,52.1,52.1,and 62.3%at 60μg/ml,respectively.Furthermore,humanized CD147 transgenic mice were susceptible to SARS-CoV-2 and its two variants,alpha and beta.When infected,these mice developed exudative alveolar pneumonia,featured by immune responses involving alveoli-infiltrated macrophages,neutrophils,and lymphocytes and activation of IL-17 signaling pathway.Mechanistically,we proposed that severe COVID-19-related cytokine storm is induced by a'spike protein-CD147-CyPA signaling axis':Infection of SARS-CoV-2 through CD147 initiated the JAK-STAT pathway,which further induced expression of cyclophilin A(CyPA);CyPA reciprocally bound to CD147 and triggered MAPK pathway.Consequently,the MAPK pathway regulated the expression of cytokines and chemokines,which promoted the development of cytokine storm.Importantly,Meplazumab could effectively inhibit viral entry and inflammation caused by SARS-CoV-2 and its variants.Therefore,our findings provided a new perspective for severe COVID-19-related pathogenesis.Furthermore,the validated universal receptor for SARS-CoV-2 and its variants can be targeted for COVID-19 treatment. | Jiejie Geng Liang Chen Yufeng Yuan Ke Wang Youchun Wang Chuan Qin Guizhen Wu Ruo Chen Zheng Zhang Ding Wei Peng Du Jun Zhang Peng Lin Kui Zhang Yongqiang Deng Ke Xu Jiangning Liu Xiuxuan Sun Ting Guo Xu Yang Jiao Wu Jianli Jiang Ling Li Kun Zhang Zhe Wang Jing Zhang Qingguo Yan Hua Zhu Zhaohui Zheng Jinlin Miao Xianghui Fu Fengfan Yang Xiaochun Chen Hao Tang Yang Zhang Ying Shi Yumeng Zhu Zhuo Pei Fei Huo Xue Liang Yatao Wang Qingyi Wang Wen Xie Yirong Li Mingyan Shi Huijie Bian Ping Zhu Zhi-Nan Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，10： | 6 |
| 8 | Surveillance on the Immune Effectiveness of Quadrivalent and Trivalent Split Influenza Vaccines—Shenzhen City and Changzhou City,China,2018–2019显示文摘Summary What is already known about this topic?Vaccinations are the most effective way to prevent influenza virus infections and severe outcomes.Influenza vaccine effectiveness can vary by seasons.What is added by this report?This report monitors the antibody level among the population over time after administration of the quadrivalent or trivalent split influenza vaccine.What are the implications for public health practice?Real-time monitoring of serum antibody changes after vaccination provides important data for the development of reasonable and effective strategies for influenza prevention and control. | Liumei Shu Jie Zhang Xiang Huo Cong Chen Shisong Fang Kexin Zong Yuanyuan Guo Yingze Zhao Jun Zhang Changjun Bao Junjie Xia Fengcai Zhu Dayan Wang Guizhen Wu George F.Gao William J.Liu | 2020 | China CDC weekly2020，2，21： | 5 |
| 9 | Two New Cytotoxic Sesquiterpenoids from Eupatorium lindleyanum DC.显示文摘二新 sesquiterpenoid 内酯,也就是 eupalinilides K 和 L,从 Eupatorium lindleyanum DC 被孤立。他们的结构被决定由光谱方法,包括第 1D-a 个 2D-NMR 系列。细胞毒素的评估证明 eupalinilide L 与 0.17 和 2.60 亩 mol/L 的 IC50 价值展出了有势力 cytotoxicityagainst P-388 和 A-549 肿瘤房间线, respectively.Eupatorium lindleyanum DC ,对中国本国,展出抗组胺和抗菌剂活动(繁体中文药 1998 的管理局的编辑委员会)。植物材料收集了省被本地居民称为“ Ye-Ma-Zhui ”的浙江的 fromSongyang 县并且为咳嗽和气管炎的治疗被用作民间药。四 germacranolides, eupalinins 广告,与对 KB 房间的重要禁止的活动,线从 E 被报导了。lindleyanum (Itoet al。1979 ) 。在以前的研究,一系列 sesquiterpenoid 内酯从这植物被孤立,其中一些展出了对二个肿瘤房间的细胞毒素的活动衬里的有势力(Huo et al.2004 ) 。在现在的学习,二新 sesquiterpenoids,也就是 eupalinilides K 和 L,从 E 的整个植物被孤立。lindleyanum。 | Jun Huo Sheng-Ping Yang Jian Ding Jian-Min Yue | 2006 | Journal of Integrative Plant Biology2006，48，4： | 5 |
| 10 | Interrogating a Fiber Bragg Grating Vibration Sensor by Narrow Line Width Light显示文摘A method to interrogate fiber Bragg grating vibration sensor by narrow line width light is demonstrated. The interrogation scheme takes advantage of the intensity modulation of narrow spectral bandwidth light, such as distributed feedback laser, when a reflection or transmission spectrum curve of an fiber Bragg grating (FBG) moves due to the strain which is applied on the sensor. The sensor's response to accelerating frequency and amplitude is measured by experiment. The factors which have impacts on the sensitivity of the interrogation system are also discussed. | Jun Chang Dian-Heng Huo Liang-Zhu Ma Xiao-Hui Liu Tong-Yu Liu Chang Wang | 2008 | Journal of Electronic Science and Technology of China2008，6，4： | 5 |
| 11 | Effect of Na Fe EDTA-Fortified Soy Sauce on Anemia Prevalence in China: A Systematic Review and Meta-analysis of Randomized Controlled Trials显示文摘Objective To assess the effect of sodium iron ethylenediaminetetraacetate(Na Fe EDTA)-fortified soy sauce on anemia prevalence in the Chinese population. Methods A systematic review was performed to identify potential studies by searching the electronic databases of Pub Med, Cochrane Library, WHO Library, High Wire, CNKI, and other sources. The selection criteria included randomized controlled trials that compared the efficacy of Na Fe EDTA-fortified soy sauce with that of non-fortified soy sauce. Anemia rates and hemoglobin levels were the outcomes of interest. Inclusion decisions, quality assessment, and data extraction were performed by two reviewers independently. A total of 16 studies met the inclusion criteria for anemia rate analysis, of which 12 studies met the inclusion criteria for hemoglobin analysis. All included studies assessed the effect of Na Fe EDTA-fortified soy sauce on anemia rates and hemoglobin concentrations. Results After the intervention, the hemoglobin concentration increased and anemia rates decreased significantly as compared with the non-fortified soy sauce groups. For anemia rates, data from 16 studies could be pooled, and the pooled estimate odds ratio was 0.25(95% CI 0.19-0.35). For hemoglobin concentrations, data from 12 studies could be pooled, and the pooled weighted mean difference was 8.81 g/L(95% CI 5.96-11.67). Conclusion Na Fe EDTA-fortified soy sauce has a positive effect on anemia control and prevention in the at-risk population. | HUO Jun Sheng YIN Ji Yong SUN Jing HUANG Jian LU Zhen Xin REGINA Moench-Pfanner CHEN Jun Shi CHEN Chun Ming | 2015 | Biomedical and Environmental Sciences2015，28，11： | 5 |
| 12 | Increased expression of cyclooxygenase-2 in first-degree relatives of gastric cancer patients显示文摘AIM: To study the expression of cyclooxygenase-2 (COX-2)in human gastric cancer tissues and their paired adjacent mucosa, as well as mucosa from gastric antrum and corpus of the first-degree relatives of the recruited cancer patients.METHODS: The expression of COX-2 mRNA in 38 patients with gastric cancer and their 29 first-degree relatives and 18 healthy controls was assessed by the real time RT-PCR.The expression of COX-2 protein was determined by Western blot.RESULTS: A marked increase in COX-2 mRNA expression was found in 20 of 37 (54%) cancerous tissues compared to their respective paired normal mucosa (P<0.001).Interestingly, increased COX-2 mRNA expression was also found in mucosa of the corpus (6/29) and antrum (13/29)of their first-degree relatives. Increased COX-2 mRNA expression was more frequently observed in the antrum biopsies from cancer patients than in the antrum biopsies from healthy controls (P<0.05). In addition, 3 of 23 (13%)patients with atrophic mucosa and 6 of 35 (17%) patients with intestinal metaplasia showed increased COX-2 mRNA expression. Furthermore, COX-2 expression increased in H pylori-positive tissues, especially in antrum mucosa.CONCLUSION: Increased COX-2 expression is involved in gastric carcinogenesis, and may be necessary for maintenance of the malignant phenotype and contribute to Helicobacterpylori-associated malignant transformation. | Jin-Ting Zhang ing-Wei Wang, Zhen-Long Zhu Xiao-Hui Huo Jian-Kun Chu Dong-Sheng Cui Liang Qiao Jun Yu | 2005 | World Journal of Gastroenterology2005，11，31： | 5 |
| 13 | A highly selective dual-channel Hg^(2+) chemosensor based on an easy to prepare double naphthalene Schiff base显示文摘A non-sulfur chemosensor based on an easy to prepare double naphthalene Schiff base is reported for the colorimetric and fluorometric dual-channel sensing of Hg2+ ions by taking advantage of the twisted intramolecular charge transfer (TICT) mechanism. This work provides a novel approach for the selective recognition of mercury ions. Moreover, this sensor serves as a potential recyclable component in sensing materials and the complex L-Hg2+ (L = 1-[(2-naphthalenylimino)methyl]-2-naphthalenol) can therefore be used as a fluorescent sensor for iodine anions. Notably, the color changes are very significant and all the recognition and recycling processes can be observed by the naked eye. | ZHANG YouMing SHI BingBing ZHANG Peng HUO JianQiang CHEN Pei LIN Qi LIU Jun WEI TaiBao | 2013 | Science China Chemistry2013，56，5： | 4 |
| 14 | Effect of high temperature annealing on the performance of MANOS charge trapping memory显示文摘We have investigated the effect of post deposition annealing (PDA) temperature of Al2O3 blocking layer on the performance of charge trapping memory capacitors. Two splits of PDA were performed in N2 ambient at 850°C/60 s and 1050°C/60 s, respectively. The 1050°C annealed capacitor could be programmed and erased normally by using Fowler-Nordheim (FN) injection. In contrast, the 850°C annealed device could not be erased, even though it could be programmed properly. By measuring the gate leakage current and the flatband voltage shift, we found the erase failure in the 850°C annealed device was due to a larger gate back-injection leakage current at Vg<0. The trend of gate leakage current was further verified in two Al/Al2O3/SiO2/p-Si control capacitors with the same PDA splits. In addition, constant voltage stress measurements on control capacitors in the FN regime showed that the change of gate leakage current followed an empirical Curie-von Schweidler law at Vg<0. The data pointed out the importance to further study the relation between PDA conditions and the defect generation properties in Al2O3 blocking layer. | JIN Lin ZHANG ManHong HUO ZongLiang YU ZhaoAn JIANG DanDan WANG Yong BAI Jie CHEN JunNing LIU Ming | 2012 | Science China(Technological Sciences)2012，55，4： | 4 |
| 15 | Study on The Method of Quantitative Analysis of Serum Ferritin and Soluble Transferrin Receptor with Protein Microarray Technology显示文摘Objective To establish and evaluate a protein microarray method for combined measurement of serum ferritin(SF) and soluble transferrin receptor(sTfR).Methods Microarrayer was used to print both anti-SF antibodies I and anti-sTfR antibodies I on each protein microarray.Anti-SF antibodies II and anti-sTfR antibodies II were used as detection antibodies and goat antibodies coupled to Cy3 were used as antibodies III.The detection conditions of the quantitative analysis method for simultaneous measurement of SF and sTfR with protein microarray were optimized and evaluated.The protein microarray was compared with commercially available traditional tests with 26 serum samples.Results By comparison experiment,mouse monoclonal antibodies were chosen as the probes and contact printing was chosen as the printing method.The concentrations of SF and sTfR probes were 0.5 mg/mL and 0.5 mg/mL respectively,while those of SF and sTfR detection antibodies were 5 μg/mL and 0.36 μg/mL respectively.Intra-and inter-assay variability was between 3.26% and 18.38% for all tests.The regression coefficients comparing protein microarray with traditional test assays were better than 0.81 for SF and sTfR.Conclusion The present study has established a protein microarray method for combined measurement of SF and sTfR. | YIN Ji Yong SUN Jing HUANG Jian LI Wen Xian HUO Jun Sheng | 2012 | Biomedical and Environmental Sciences2012，25，4： | 4 |
| 16 | Rapid Identification of Legionella Pathogenicity by Surface-Enhanced Raman Spectroscopy显示文摘Objective To establish Surface-enhanced Raman Spectroscopy(SERS) can be used as a rapid and reliable method to distinguish virulent strain and mild strain of L. pneumophila. Methods We isolated and characterized of bacterial strains from ATCC and water samples strains, while we analyzed data from SERS technology using gold nanoparticles as a base and cell infections were employed to rapidly detect L. pneumophila strains. Origin 8.0 was used to collect Raman spectra, smooth and homogenize data, and to contrast spectra. Principal component analysis(PCA) was conducted to discriminate differences between groups using the multivariate analysis package Py Chem 3.0.5. Results Our results indicated that the peaks of high virulence strains reached ≥4000. This criterion was verified by subsequent cell experiments. In addition, we also conducted SERS rapid identification on the virulence of several collected clinical strains and obtained accurate results. Conclusion The present study indicates that the established SERS protocol can be used as a rapid and reliable method to distinguish virulent and mildly virulent strains of L. pneumophila, which can be further used in clinical samples. | LI Jing QIN Tian JIA Xiao Xiao DENG Ai Hua ZHANG Xu FAN Wen Hui HUO Shuai Dong WEN Ting Yi LIU Wen Jun | 2015 | Biomedical and Environmental Sciences2015，28，6： | 4 |
| 17 | Toward an ideal electrical resistance strain gauge using a bare and single straight strand metallic glassy fiber显示文摘Electrical resistance strain gauges(SGs) are useful tools for experimental stress analysis and the strain sensing elements in many electromechanical transducers including load cells,pressure transducers,torque meters,accelerometers,force cells,displacement transducers and so forth.The commonly used commercial crystalline strain sensing materials of SGs are in the form of wire or foil of which performance and reliability is not good enough due to their low electrical resistivity and incapacity to get thin thickness.Smaller SGs with single straight strand strain sensing materials,which are called ideal SG,are highly desirable for more than seven decades since the first SG was invented.Here,we show the development of a type of minuscule length scale strain gauge by using a bare and single straight strand metallic glassy fiber(MGF) with high resistivity,much smaller lengthscale,high elastic limits(2.16%) and especially the super piezoresistance effect.We anticipate that our metallic glassy fiber strain gauge(MGFSG),which moves toward the ideal SGs,would have wide applications for electromechanical transducers and stress analysis and catalyze development of more micro-and nanoscale metallic glass applications. | YI Jun HUO LiShan ZHAO DeQian PAN MinXiang BAI HaiYang WANG WeiHua | 2012 | Science China(Physics,Mechanics & Astronomy)2012，55，4： | 4 |
| 18 | Simulation of stray grain formation in Ni-base single crystal turbine blades fabricated by HRS and LMC techniques显示文摘The simulation models of the thermal and macrostructural evolutions during directional solidification of Ni-base single crystal(SX) turbine blades under high rate solidification(HRS) and liquid metal cooling(LMC) have been constructed using Pro CAST software, coupled with a 3D Cellular Automaton Finite Element(CAFE) model. The models were used to investigate the tendencies of stray grain(SG) formation in the platform region of turbine blades fabricated by HRS and LMC techniques. The results reveal that the LMC technique can prohibit SG formation by smoothing the concaved isotherm and in turn alleviating the undercooling in the platform ends to let the dendrites fill up the undercooled zone before SG nucleation. The simulation results agreed well with the experimental results, indicating that these models could be used to analyze the macrostructural evolution or to optimize process parameters to suppress SG formation. Using these models, the critical withdrawal rate for casting SX turbine blades without SG formation were determined to be around 75 μm·s^(-1) and 100 μm·s^(-1) for HRS and LMC respectively, suggesting that LMC can be used as an efficient technique in fabricating SX turbine blades without any SG defect formation. | Ya-feng Li Lin Liu Tai-wen Huang Miao Huo Jun-sheng He Jun Zhang Heng-zhi Fu | 2017 | China Foundry2017，14，2： | 4 |
| 19 | Experimental and numerical simulation of bird-strike performance of lattice-material-infilled curved plate显示文摘The anti-bird-strike performance of a lattice-material-infilled curved plate is investigated herein.Since automatically filling the curved structure by classical lattice material filling methods will cause a large number of manufacturing defects,a space-dependent lattice material filling method for the curved plate is firstly proposed in this paper Next,using a face-centered cubic lattice,a lattice-material-infilled test piece with a hollow ratio of 40.8%is built.The test pieces are manufactured via additive manufacturing using titanium alloy.In bird-strike experimental tests,the test pieces are crashed against gelatin birds at an impact velocity of 200 m/s.Dynamic strain gauges are used to record the crash history and the results are discussed.Furthermore,a numerical analysis to simulate the bird-strike experiment is performed.The results from the experimental tests and numerical simulation agree well.This work shows that the lattice-material-infilled curved plate yields promising bird-strike resistance.Therefore,lattice-infilled materials are feasible for protecting aerospace components against bird-strike as well as for reducing the component weight. | Jun YAN Chenguang ZHANG Sixu HUO Xianghai CHAI Zhihui LIU Kun YAN | 2021 | Chinese Journal of Aeronautics2021，34，8： | 4 |
| 20 | Replacement of Forage Fiber Sources with Dried Distillers Grains with Solubles and Corn Germ Meal in Holstein Calf Diets显示文摘This study was carried out to determine the effect of replacement of forage fiber sources from alfalfa and Leymus chinensis with nonforage fiber sources(NFFS) from dried distillers grains with solubles and corn germ meal on calves growth, rumen development and blood parameters. 48 female and 12 male calves((110.55±15.36) kg of body weight and 12 wk of age) were assigned randomly to four dietary treatments(15 calves/treatment) in a completely randomized design. Experimental diets were: 0% NFFS(control), 9% NFFS(group 1), 18% NFFS(group 2), 27% NFFS(group 3), and contained equivalent neutral detergent fiber and total digestible nutrients, respectively. The dry matter intake was similar among diets, averaging 3.33 kg d-1, and no differences were detected for body weight, withers height, body length and heart girth. In addition, the development of rumen, reticulum, omasum and abomasum also were similar among diets. Dry matter, crude protein, and neutral detergent fiber digestibilities increased with the increasing levels of NFFS in the experimental diets, but had no significant effect. Blood urea nitrogen, total protein and glubulin were not affected by the dietary treatment, but group 3 resulted in the highest(P<0.05) concentrations of glucose and the lowest(P<0.05) concentrations of triglycerides and albumin. In conclusion, dried distillers grains with solubles(DDGS) and corn germ meal(CGM) were available and alternative fiber sources for Holstein calf diets. | XU Jun HOU Yu-jie ZHAO Guo-qi YU Ai-bing SU Yan-jing HUO Yong-jiu ZHU Jian-ming | 2014 | Journal of Integrative Agriculture2014，13，8： | 4 |
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