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| 1 | Simulation analysis and match experiment on negative and positive pressures of pneumatic precision metering device for rapeseed显示文摘Positive and negative pressures determine the performance of pneumatic precision metering device for rapeseed.In order to investigate the relationship between positive and negative pressures of nozzles,fluid models of chamber were developed to simulate the airflow,and the k-εturbulence model was conducted to capture the pressure and velocity of nozzles.Through these efforts linear models were achieved.Meanwhile,the three-factor factorial split-split experiment was designed with negative pressure,positive pressure and the rotating speeds varying from-1000 to-4500 Pa,50 to 250 Pa and 10 to 45 r/min,respectively.The mathematical models were developed through employing the stepwise regression method.The sequence of influential factors on the quality of feed index was positive pressure,negative pressure and rotating speed.To obtain the match regulation of negative and positive pressures with“good”performance,the ratio coefficient K of negative and positive pressures was introduced to build mathematical models.Models relating ratio coefficient K with positive pressure were fitted in different rotating speeds.The results showed that the ratio coefficient was matchedГ∈[f1(x),f2(x)]from the fitting equations with the rotating speed of 10-30 r/min;while the rotating speed has greater influence when it was 35-40 r/min and the setsΛ∈[g1(x),g2(x)]were achieved,where x∈[100,250].This study could be conducted to adjust the rotating speed of the pneumatic system to optimize the ideal performance of the seeder. | Yu Jiajia Liao Yitao Cong Jinling Yang Song Liao Qingxi | 2014 | International Journal of Agricultural and Biological Engineering2014，7，3： | 21 |
| 2 | Modeling spatio-temporal distribution of soil moisture by deep learning-based cellular automata model显示文摘Soil moisture content (SMC) is a key hydrological parameter in agriculture,meteorology and climate change,and understanding of spatio-temporal distributions of SMC in farmlands is important to address the precise irrigation scheduling.However,the hybrid interaction of static and dynamic environmental parameters makes it particularly difficult to accurately and reliably model the distribution of SMC.At present,deep learning wins numerous contests in machine learning and hence deep belief network (DBN) ,a breakthrough in deep learning is trained to extract the transition functions for the simulation of the cell state changes.In this study,we used a novel macroscopic cellular automata (MCA) model by combining DBN to predict the SMC over an irrigated corn field (an area of 22 km^2) in the Zhangye oasis,Northwest China.Static and dynamic environmental variables were prepared with regard to the complex hydrological processes.The widely used neural network,multi-layer perceptron (MLP) ,was utilized for comparison to DBN.The hybrid models (MLP-MCA and DBN-MCA) were calibrated and validated on SMC data within four months,i.e.June to September 2012,which were automatically observed by a wireless sensor network (WSN) .Compared with MLP-MCA,the DBN-MCA model led to a decrease in root mean squared error (RMSE) by 18%.Thus,the differences of prediction errors increased due to the propagating errors of variables,difficulties of knowing soil properties and recording irrigation amount in practice.The sequential Gaussian simulation (s Gs) was performed to assess the uncertainty of soil moisture estimations.Calculated with a threshold of SMC for each grid cell,the local uncertainty of simulated results in the post processing suggested that the probability of SMC less than 25% will be difference in different areas at different time periods.The current results showed that the DBN-MCA model performs better than the MLP-MCA model,and the DBN-MCA model provides a powerful tool for predicting SMC in highly non-linear forms.Moreover,because modeling soil moisture by using environmental variables is gaining increasing popularity,DBN techniques could contribute a lot to enhancing the calibration of MCA-based SMC estimations and hence provide an alternative approach for SMC monitoring in irrigation systems on the basis of canals. | SONG Xiaodong ZHANG Ganlin LIU Feng LI Decheng ZHAO Yuguo YANG Jinling | 2016 | Journal of Arid Land2016，8，5： | 20 |
| 3 | Versatile Nucleotides Substitution in Plant Using an Improved Prime Editing System显示文摘Dear Editor,Base editors(BEs)based on the CRISPR/Cas9 system,including cytosine base editors and adenine base editors,which can efficiently perform four transition mutations(C·G-to-T·A and A·T-to-G·C),have been well studied and widely used to produce base mutations in a variety of organisms,including in plants such as rice. | Wen Xu Chengwei Zhang Yongxing Yang Si Zhao Guiting Kang Xiaoqing He Jinling Song Jinxiao Yang | 2020 | Molecular Plant2020，13，5： | 20 |
| 4 | Discriminated sgRNAs-Based SurroGate System Greatly Enhances the Screening Efficiency of Plant Base-Edited Cells显示文摘The development of CRISPR/Cas9-mediated base editing has made genomic modification more efficient. However, selection of genetically modified cells from millions of treated cells, especially plant cells, is still challenging. In this study, an efficient surrogate reporter system based on a defective hygromycin resistance gene was established in rice to enrich base-edited cells. After step-by-step optimization, the Discriminated sgRNAs-based SurroGate system (DisSUGs) was established by artificially differentiating the editing abilities of a wild-type single guide RNA (sgRNA) targeting the surrogate reporter gene and an enhanced sgRNA targeting endogenous sites. The DisSUGs enhanced the efficiency of screening base-edited cells by 3- to 5-fold for a PmCDA1-based cytosine-to-tyrosine base editor (PCBE), and 2.5- to 6.5-fold for an adenine base editor (ABE) at endogenous targets. These targets showed editing efficiencies of <25% in the conventional systems. The DisSUGs greatly enhanced the frequency of homozygous substitutions and expanded the activity window slightly for both a PCBE and an ABE. Analyses of the total number of single-nucleotide variants from whole-genome sequencing revealed that, compared with the no-enrichment PCBE strategy, the DisSUGs did not alter the frequency of genome-wide sgRNA-independent off-target mutations, but slightly increased the frequency of target-dependent off-target mutations. Collectively, the DisSUGs developed in this study greatly enhances the efficiency of screening plant base-edited cells and will be a useful system in future applications. | Wen Xu Yongxing Yang Ya Liu Guiting Kang Feipeng Wang Lu Li Xinxin Lv Si Zhao Shuang Yuan Jinling Song Ying Wu Feng Feng Xiaoqing He Chengwei Zhang Wei Song Jiuran Zhao Jinxiao Yang | 2020 | Molecular Plant2020，13，1： | 8 |
| 5 | Lactic Acid Assisted Solvothermal Synthesis of BiOClxI1-x Solid Solutions as Excellent Visible Light Photocatalysts显示文摘A series of BiOClxI1-x(x=0,0.1,0.3,0.5,0.7,0.9,1.0)photocatalysts was firstly prepared by means of a facile solvothermal route with the help of lactic acid.The measured results show that the morphologies of the as-prepared samples are similar sheets with different thickness and diameters.Thinner nanosheets assembled flower-like BiOCI0.5I0.5 solid solution exhibited the highest photocatalytic activity and stability among the prepared samples for the degradation of methylene blue(MB)and methyl orange(MO)under the illumination of visible light.The excellent photocatalytic properties of BiOCI0.5I0.5 could be attributed to the high specific surface area,the suita-ble band gap energy and the lower recombination rate of the electrons and holes.In addition,catalyst BiOCI0.5I0.5 was further used to degradate a more complicated mixed dye(MCH-RhB+MB)system under visible light,displaying an excellent photocatalytic activity.Finally,the photocatalytic mechanism of catalyst BiOCI0.5I0.5 to degradate colorful dyes was proposed.The trapping experiments of active species indicated that the holes are the main active species for the degradation of the mixed dyes. | DUAN Chenglin SONG Jinling WANG Baoying LI Lun WANG Ruifen ZHANG Bangwen | 2019 | Chemical Research in Chinese Universities2019，35，2： | 4 |
| 6 | Expanding base editing scope to near-PAMless with engineered CRISPR/Cas9 variants in plants显示文摘Dear Editor,Base editors(BEs),including cytosine base editor(CBE)and adenine base editor(ABE),have been widely used to generate irreversible nucleotide substitution in plants and animals.However,their wide applications are largely hindered by the strict NG protospacer adjacent motif(PAM)sequences recognized by Streptococcus pyogenes Cas9(SpCas9)and its engineered variants,such as SpCas9-NG and xCas9(Hua et al.,2019;Ren et al.,2019;Wu et al.,2019;Zhong et al.,2019;Zhang et al.,2020).Most recently,it was reported that three new SpCas9 variants,SpCas9-NRRH,SpCas9-NRTH,and SpCas9-NRCH,could recognize non-G PAMs(NRNH,where R is A or G and H is A,C,or T)in human cells(Miller et al.,2020).Meanwhile,SPRY,another new SpCas9 variant,was developed to greatly expand the editing scope of BEs to nearly PAMless(Walton et al.,2020).In this study,we generated a series of efficient BE toolkits and almost achieved C-to-T mutation without PAM restriction except for NTG PAM,and largely expanded A-to-G mutation scope in stable transformed rice,providing a reference for application in other plants. | Chengwei Zhang Yao Wang Feipeng Wang Si Zhao Jinling Song Feng Feng Jiuran Zhao Jinxiao Yang | 2021 | Molecular Plant2021，14，2： | 4 |
| 7 | Preparation and Adsorption Properties of Biomass Activated Carbon from Ginger Stems显示文摘Biomass activated carbon(BAC)was produced from ginger stems by carbonization and activation presented high specific surface areas and mesoporous structures.The carbonization temperature of the ginger stems were controlled within 500~900℃.The optimal carbonization condition is as follows:carbonization temperature of 700℃,carbonization time of 6 h.The determined optimum activation condition is:temperature of 800℃,activator of KOH and carbonized product/alkali ratio of 1:4(w/w).The carbonization yield,BAC yield and Brunauer-Emmett-Teller(BET)surface area were measured and the adsorption performance of BAC to nitrogen was investigated.The results showed that the nitrogen adsorption isotherm curve was as type I isotherm.It was finally determined that the BET surface area was 660 m2/g under the abovementioned optimal conditions of carbonization and activation.The FESEM analysis indicates that the obtained BAC is of micropore structure. | Jinling Liu Xiaoming Song Shanshan Gao Fushan Chen | 2019 | Paper And Biomaterials2019，4，4： | 3 |
| 8 | Developing high-efficiency base editors by combining optimized synergistic core components with new types of nuclear localization signal peptide显示文摘The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base editor(ABE) allow generating precise and irreversible base mutations in a programmable manner and have been used in many different types of cells and organisms. However, their applications are limited by low editing efficiency at certain genomic target sites or at specific target cytosine(C) or adenine(A) residues. Using a strategy of combining optimized synergistic core components, we developed a new multiplex super-assembled ABE(sABE) in rice that showed higher base-editing efficiency than previously developed ABEs. We also designed a new type of nuclear localization signal(NLS) comprising a FLAG epitope tag with four copies of a codon-optimized NLS(F4NLS^(r2)) to generate another ABE named F4NLS-sABE. This new NLS increased editing efficiency or edited additional A at several target sites. A new multiplex super-assembled CBE(sCBE) and F4NLS^(r2) involved F4NLS-sCBE were also created using the same strategy. F4NLS-sCBE was proven to be much more efficient than sCBE in rice. These optimized base editors will serve as powerful genome-editing tools for basic research or molecular breeding in rice and will provide a reference for the development of superior editing tools for other plants or animals. | Feipeng Wang Chengwei Zhang Wen Xu Shuang Yuan Jinling Song Lu Li Jiuran Zhao Jinxiao Yang | 2020 | The Crop Journal2020，8，3： | 3 |
| 9 | Isolation of a novel characterized Issatchenkia terricola from red raspberry fruits on the degradation of citric acid and enrichment of flavonoid and volatile profiles in fermented red raspberry juice显示文摘High content of citric acid in red raspberry juice leads to poor sensory experience. This study developed a feasible method to degrade citric acid in red raspberry juice using a novel characterized Issatchenkia terricola WJL-G4 isolated from red raspberry fruits. I. terricola WJL-G4 exhibited a potent capability of reducing the citric acid contents from(22.8 ± 0.08) g/L to(6.2 ± 0.02) g/L within 36 h fermentation, then completely depleted after 48 h. Furthermore, the contents of phenolic compound, including neochlorogenic acid, p-coumaric acid, raspberry ketone, and rutin significantly increased after 36 h fermentation. Fermentation increased total flavonoid contents in red raspberry juice, compared to that in control group. Volatile profiles exhibited to be enriched after fermentation, which contributed to the improvement of the juice taste. Our findings showed that I. terricola WJL-G4 can be applied in deacidification, enrichment of flavonoid compounds and volatile profiles in fermented red raspberry juice.. | Ying Jiang Ting Luo Ying Tang Sirui Chen Hui Ni Qihe Chen Xingshun Song Yihong Bao Zeyuan Deng Jinling Wang | 2022 | Food Science and Human Wellness2022，11，4： | 3 |
| 10 | High Spatial Resolution and High Temporal Frequency(30-m/15-day) Fractional Vegetation Cover Estimation over China Using Multiple Remote Sensing Datasets:Method Development and Validation显示文摘High spatial resolution and high temporal frequency fractional vegetation cover(FVC) products have been increasingly in demand to monitor and research land surface processes. This paper develops an algorithm to estimate FVC at a 30-m/15-day resolution over China by taking advantage of the spatial and temporal information from different types of sensors: the 30-m resolution sensor on the Chinese environment satellite(HJ-1) and the 1-km Moderate Resolution Imaging Spectroradiometer(MODIS). The algorithm was implemented for each main vegetation class and each land cover type over China. First, the high spatial resolution and high temporal frequency normalized difference vegetation index(NDVI) was acquired by using the continuous correction(CC) data assimilation method. Then, FVC was generated with a nonlinear pixel unmixing model. Model coefficients were obtained by statistical analysis of the MODIS NDVI. The proposed method was evaluated based on in situ FVC measurements and a global FVC product(GEOV1 FVC). Direct validation using in situ measurements at 97 sampling plots per half month in 2010 showed that the annual mean errors(MEs) of forest, cropland, and grassland were-0.025, 0.133, and 0.160, respectively, indicating that the FVCs derived from the proposed algorithm were consistent with ground measurements [R2 = 0.809,root-mean-square deviation(RMSD) = 0.065]. An intercomparison between the proposed FVC and GEOV1 FVC demonstrated that the two products had good spatial–temporal consistency and similar magnitude(RMSD approximates 0.1). Overall, the approach provides a new operational way to estimate high spatial resolution and high temporal frequency FVC from multiple remote sensing datasets. | Xihan MU Tian ZHAO Gaiyan RUAN Jinling SONG Jindi WANG Guangjian YAN Tim RMCVICAR Kai YAN Zhan GAO Yaokai LIU Yuanyuan WANG | 2021 | Journal of Meteorological Research2021，35，1： | 3 |
| 11 | A 18FDG PET/CT-based volume parameter is a predictor of overall survival in patients with local advanced gastric cancer显示文摘Objective: The present study investigated the prognosis value of preoperative fluorodeoxyglucose(FDG)positron emission tomography/computed tomography(PET/CT) in patients with local advanced gastric cancer(LAGC).Methods: In total, 144 patients [median age 63(range: 48-80) years old] with LAGC underwent 18 F-FDG PET/CT prior to any treatment. The maximum standardized uptake values(SUVmax), mean standardized uptake values(SUVmean), metabolic tumor volume(MTV) and total lesion glycolysis(TLG) of the primary lesion were measured on PET/CT and correlated with clinicopathological features and survival.Results: Significant differences in SUVmean, SUVmax, MTV and TLG were found according to Lauren’s classification, histologic grade and T category(P<0.05). During the 26.5-month follow-up, 51(35.4%) patients died and 70(48.6%) exhibited disease progression. The optimal thresholds of MTV and TLG were 15.1 cm^3 and 47.3 cm^3, respectively. The 3-year progression-free survival(PFS) and overall survival(OS) for patients with high TLG values were 30% and 38% compared to 38% and 47% for low TLG values, respectively(P<0.05). Univariate and multifactor analyses demonstrated that lymph node metastasis and T stage were independent prognostic factors for PFS;T stage, histologic grade and TLG were independent prognostic factors for OS(P<0.05). Molecular markers had no relationship with patient’s outcomes.Conclusions: Metabolic activity of primary gastric tumors from 18 F-FDG PET/CT is a prognostic factor in patients with LAGC. | Jinling Song Zhongwu Li Puyun Chen Jiangyuan Yu Feng Wang Zhi Yang Xuejuan Wang | 2019 | Chinese Journal of Cancer Research2019，31，4： | 2 |
| 12 | Highly efficient CRISPR-SaKKH tools for plant multiplex cytosine base editing显示文摘Base editing, as an expanded clustered regularly interspaced short palindromic repeats(CRISPR)-Cas genome editing strategy, permits precise and irreversible nucleotide conversion. SaKKH, an efficient variant of a Cas9 ortholog from Staphylococcus aureus(SaCas9), is important in genome editing because it can edit sites with HHHAAT protospacer adjacent motif(PAM) that the canonical Streptococcus pyogenes Cas9(SpCas9) or its variants(e.g. xCas9, Cas9-NG) cannot. However, several technical parameters of SaKKH involved base editors have not been well defined and this uncertainty limits their application. We developed an effective multiplex cytosine base editor(SaKKHn-pBE) and showed that it recognized NNARRT, NNCRRT, NNGRGT, and NNTRGT PAMs. Based on 27 targets tested, we defined technical parameters of SaKKHn-pBE including the editing window, the preferred sequence context, and the mutation type. The editing efficiency was further improved by modification of the SaKKH sgRNA. These advances can be applied in future research and molecular breeding in rice and other plants. | Chengwei Zhang Feipeng Wang Si Zhao Guiting Kang Jinling Song Lu Li Jinxiao Yang | 2020 | The Crop Journal2020，8，3： | 1 |
| 13 | Mapping Soil Organic Carbon Using Local Terrain Attributes: AComparison of Different Polynomial Models显示文摘Local terrain attributes,which are derived directly from the digital elevation model,have been widely applied in digital soil mapping.This study aimed to evaluate the mapping accuracy of soil organic carbon(SOC) concentration in 2 zones of the Heihe River in China,by combining prediction methods with local terrain attributes derived from different polynomial models.The prediction accuracy was used as a benchmark for those who may be more concerned with how accurately the variability of soil properties is modeled in practice,rather than how morphometric variables and their geomorphologic interpretations are understood and calculated.In this study,2 neighborhood types(square and circular) and 6 representative algorithms(Evans-Young,Horn,Zevenbergen-Thorne,Shary,Shi,and Florinsky algorithms) were applied.In general,35 combinations of first-and second-order derivatives were produced as candidate predictors for soil mapping using two mapping methods(i.e.,kriging with an external drift and geographically weighted regression).The results showed that appropriate local terrain attribute algorithms could better capture the spatial variation of SOC concentration in a region where soil properties are strongly influenced by the topography.Among the different combinations of firstand second-order derivatives used,there was a best combination with a more accurate estimate.For different prediction methods,the relative improvement in the two zones varied between 0.30% and 9.68%.The SOC maps resulting from the higher-order algorithms(Zevenbergen-Thorne and Florinsky) yielded less interpolation errors.Therefore,it was concluded that the performance of predictive methods,which incorporated auxiliary variables,could be improved by attempting different terrain analysis algorithms. | SONG Xiaodong LIU Feng ZHANG Ganlin LI Decheng ZHAO Yuguo YANG Jinling | 2017 | Pedosphere2017，27，4： | 1 |
| 14 | Structure and protective effect on UVB-induced keratinocyte damage of fructan from white garlic显示文摘 | CHEN Jinling CHEONG K L SONG Ziliang | 2013 | Carbohydrate Polymers2013，92，： | 1 |
| 15 | Passivity analysis of discrete-time stochastic neural net- works with time-varying delays 显示文摘 | SONG Qiankun LIANG Jinling WANG Zidong | 2009 | Neurocomputing2009，2，： | 1 |
| 16 | Accumulation, transportation, and distribution of tetracycline and cadmium in rice显示文摘Co-exposure to heavy metal and antibiotic pollution might result in complexation and synergistic interactions,affecting rice growth and further exacerbating pollutant enrichment.Therefore,our study sought to clarify the influence of different Tetracycline (TC) and Cadmium(Cd) concentration ratios (both alone and combined) on rice growth,pollutant accumulation,and transportation during the tillering stage in hydroponic system.Surprisingly,our findings indicated that the interaction between TC and Cd could alleviate the toxic effects of TC/Cd on aerial rice structures and decrease pollutant burdens during root elongation.In contrast,TC and Cd synergistically promoted the accumulation of TC/Cd in rice roots.However,their interaction increased the accumulation of TC in roots while decreasing the accumulation of Cd when the toxicant doses increased.The strong affinity of rice to Cd promoted its upward transport from the roots,whereas the toxic effects of TC reduced TC transport.Therefore,the combined toxicity of the two pollutants inhibited their upward transport.Additionally,a low concentration of TC promoted the accumulation of Cd in rice mainly in the root tip.Furthermore,a certain dose of TC promoted the upward migration of Cd from the root tip.Laser ablation-inductively coupled plasma mass spectrometry demonstrated that Cd mainly accumulated in the epidermis and stele of the root,whereas Fe mainly accumulated in the epidermis,which inhibited the absorption and accumulation of Cd by the rice roots through the generation of a Fe plaque.Our findings thus provide insights into the effects of TC and Cd co-exposure on rice growth. | Chunfeng He Jini Zhou Chen Yang Zhiyi Song Junheng He Ziqing Huang Yurong Deng Jinling Wang Yu Xiong Zhi Dang | 2023 | Journal of Environmental Sciences2023，，4： | 1 |
| 17 | Existence form of lathanum and its improving mechanism of visible-light-driven La-F co-doped TiO2显示文摘The existence form and effect of La on the phase composition,morphology,recombination of photocarriers,and optical absorptivity of La-F codoped TiO2 were investigated.Experimental results indicate that all the phase composition of samples is anatase TiO2,the grain sizes decrease with the increasing of La content The catalysts have a well-defined spherical structure with an average size of 12-14 nm,and the doping elements are uniformly dispersed.Compared with pure TiO2,the absorption edge of La1.5F5-TiO2 red shifts from 388 to 437 nm,accordingly the energy gap(Eg) reduces from 32 to 2.84 eV,Besides,the recombination rate of electron-holes in La1.5F5-TiO2 is the weakest among the prepared samples,and the specific surface area of La1.5F5-TiO2 reaches 105.27 m2/g.The apparent reaction rate constant k of La1.5F5-TiO2 for methylene blue(MB) degradation under visible light obtained from the apparent first-order model achieves 0.0166±0.52 min-1,which is greater than 0.0033±0.09 min 1 of TiO2.Moreover,high-resolution transmission electron microscopy(HRTEM) observation reveals that there coexist La2O3 particles in the co-doped TiO2. | Ruifen Wang Shengli An Jing Zhang Jinling Song Fuming Wang | 2020 | Journal of Rare Earths2020，38，1： | 1 |
| 18 | How to select optimal K-value for K-anonymity model显示文摘 | Huang Liming Zhang Chao Song Jinling | | 0，，01： | 1 |
| 19 | Dynamic game analysis on knowledge-sharing and knowledge-spillover in competi- tive alliances 显示文摘 | Xiaozhong Song Jinling Li Nan Xu | 2008 | Proceedings of the 2008 International Con- ference on Computer Science and Software Engineering2008，12，15： | 1 |
| 20 | Antrinflam matory activity of the major corrstituents of Lonicera japonica显示文摘 | Song JinLe e | 1995 | Arch Pharm Res1995，18，2： | 1 |
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