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| 1 | Gene therapy: light is finally in the tunnel显示文摘After two decades of ups and downs,gene therapy has recently achieved a milestone in treating patients with Leber’s congenital amaurosis(LCA).LCA is a group of inherited blinding diseases with retinal degeneration and severe vision loss in early infancy.Mutations in several genes,including RPE65,cause the disease.Using adenoassociated virus as a vector,three independent teams of investigators have recently shown that RPE65 can be delivered to retinal pigment epithelial cells of LCA patients by subretinal injections resulting in clinical benefits without side effects.However,considering the whole field of gene therapy,there are still major obstacles to clinical applications for other diseases.These obstacles include innate and immune barriers to vector delivery,toxicity of vectors and the lack of sustained therapeutic gene expression.Therefore,new strategies are needed to overcome these hurdles for achieving safe and effective gene therapy.In this article,we shall review the major advancements over the past two decades and,using lung gene therapy as an example,discuss the current obstacles and possible solutions to provide a roadmap for future gene therapy research. | Huibi Cao Robert S.Molday Jim Hu | 2011 | Protein & Cell2011，2，12： | 9 |
| 2 | Regulation of epithelium-specific Ets-like factors ESE-1 and ESE-3 in airway epithelial cells: potential roles in airway inflammation显示文摘航线发炎是许多呼吸障碍的特点,例如气喘和膀胱的纤维变性。在发炎触发的航线基因表示的变化在这些疾病的致病起一个关键作用。基因连接研究建议 ESE-2 和 ESE-3,编码上皮特定的 Ets-domain-containing 抄写因素,是候选人气喘危险性基因。我们这里报导 et 家庭抄写因素 ESE-1 的另一个成员的表示,以及 ESE-3,起来在支气管的上皮的房间线由煽动性的 cytokines interleukin-1beta (IL-1beta ) 和肿瘤坏死 factor-alpha (TNF-alpha ) 调整了。有 IL-1beta 和 TNF-alpha 的这些房间的处理为 ESE-1 和 ESE-3 导致了信使 rna 表示的戏剧的增加。我们证明导致的表示被抄写因素 NF-kappaB 的激活调停。我们描绘了 ESE-1 和 ESE-3 倡导者并且识别了为导致 cytokine 的表达式被要求的 NF-kappaB 有约束力的序列。另外,我们也表明那 ESE-1 在上面调整 ESE-3 表示, down 由 cytokines 调整它的自己的正式就职。最后,我们在 Elf3 显示出那(对人的 ESE-1 相应) 猛烈老鼠,煽动性的 cytokine interleukin-6 (IL-6 ) 的表示是调整的 down。我们的调查结果建议 ESE-1 和 ESE-3 在航线发炎起一个重要作用。 | Jing Wu Rongqi Duan Huibi Cao Deborah Field Catherine M Newnham David R Koehler Noe Zamel Melanie A Pritchard Paul Hertzog Martin Post A Keith Tanswell Jim Hu | 2008 | Cell Research2008，18，6： | 6 |
| 3 | IL-17RD (Sef or IL-17RLM) interacts with IL-17 receptor and mediates IL-17 signaling显示文摘Interleukin-17 (IL-17 或 IL-17A ) 生产是 TH17 房间的一个特点,贡献多重自体免疫、煽动性的疾病的致病的 CD4+ T 淋巴细胞的一个新唯一的系。IL-17 受体(IL-17R 或 IL-17RA ) 为 IL-17 生物活动是必要的。新兴的数据建议 heteromeric 或 homomeric 受体建筑群的形成为 IL-17 发信号被要求。这里,我们证明孤儿受体 IL-17RD (Sef,类似的表示到 FGF 基因或 IL-17RLM ) 被联系并且有 IL-17R 的 colocalized。重要地, IL-17RD 调停 IL-17 发信号,用一个酶记者评估了由 24p3 的本国的倡导者开车, IL-17 目标基因。另外,主导否定地缺乏细胞内部的领域的 IL-17RD 异种压制 IL-17R-mediated IL-17 发信号。而且,象 IL-17R 一样的 IL-17RD 与 TRAF6 被联系,一个 IL-17R 下游的分子。这些结果显示 IL-17RD 是表明建筑群的 IL-17 受体的部分,因此为通过 heteromeric 或 homomeric 受体建筑群发信号的 IL-17 提供新奇证据。 | Zhili Rong Anan Wang Zhiyong Li Yongming Ren Long Cheng Yinghua Li Yinyin Wang Fangli Ren Xiaoning Zhang Jim Hu Zhijie Chang | 2009 | Cell Research2009，19，2： | 6 |
| 4 | Quercetin ameliorates paclitaxel-induced neuropathic pain by stabilizing mast cells, and subsequently blocking PKCε-dependent activation of TRPV1显示文摘瞄准:严重痛苦的感觉神经病经常发生在 paclitaxel 化疗期间。,后来, paclitaxel 能激活桅杆房间和 basophils 橡黄素, polyphenolic flavonoid 在各种各样的植物包含了,它能明确地作为桅杆房间 stabilizer 禁止组织安版本。在这研究,我们探索橡黄素是否能改善导致 paclitaxel 的神经病的疼痛和阐明的位于 \O 下面 mechanisms.Methods:组织安版本上的橡黄素抑制被从白血病(RBL-2H3 ) 房间与 paclitaxel 刺激了的老鼠 basophilic 检测组织安版本在 vitro 验证(10 μ mol/L ) 。在里面在里面 vivo 实验,老鼠和老鼠收到了橡黄素(20, 40 mg·为 40 和 12 d 的 kg -1·d-1) 分别地。同时,动物与 paclitaxel 被注射(2 mg/kg, ip ) d 上的四次 1, 3, 5 和 7。热痛觉过敏和机械 allodynia 在不同时间点被评估。动物是 euthanized 和针的绳索,背面的根中心为分析 PKCε 被收获;并且 TRPV1 表示铺平。血浆组织安层次在 d 上在老鼠被估计 31 .Results:有橡黄素的预告的处理(3, 10, 30 μ mol/L ) 从在 vitro 的刺激 paclitaxel 的 RBL-2H3 房间的 dose-dependently 禁止的过多的组织安版本,和橡黄素管理显著地在对待 paclitaxel 的老鼠压制了高血浆组织安层次。橡黄素管理 dose-dependently 在对待 paclitaxel 的老鼠和老鼠为热痛觉过敏和机械 allodynia 提起了阀值。而且,橡黄素管理 dose-dependently 压制了 PKCε 的增加的表示层次;并且在对待 paclitaxel 的老鼠和老鼠的针的绳索和 DRG 的 TRPV1。而且,橡黄素管理可以禁止了 PKCε 的 translocation;从细胞质到在对待 paclitaxel 的 rats.Conclusion 的针的绳索和 DRG 的膜:我们的结果揭示导致 paclitaxel 的外部神经病的内在的机制并且为对待这个副作用表明橡黄素的治疗学的潜力。 | Wei GAO Yan ZAN Zai-jie Jim WANG Xiao-yu HU Fang HUANG | 2016 | Acta Pharmacologica Sinica2016，37，9： | 5 |
| 5 | 经自然腔道取标本手术(NOSES)治疗结直肠癌的国际共识显示文摘近年来,经自然腔道取标本手术(NOSES)治疗结直肠癌已引起了广泛关注。NOSES应用于结直肠手术的潜在优势包括减轻术后疼痛,减少伤口并发症,减少对术后镇痛的需求,加快肠道功能的恢复,缩短住院时间,更好的美容效果,以及给患者带来了更佳的心理状态。尽管NOSES的手术创伤显著减轻,然而该技术也存在一些潜在问题。尤其随着这项新技术的兴起,人们对其无菌的把控、肿瘤学结果和病例选择等问题表示出关切。因此,迫切需要一部操作指南来规范NOSES手术在结直肠癌中的开展。经过国际NOSES联盟全体成员的三轮讨论,最终形成了该部共识。这一共识有助于NOSES在全球范围内的长远发展。 | Xu Guan Zheng Liu Antonio Longo Jian-Chun Cai William Tzu-Liang Chen Lu-Chuan Chen Ho-Kyung Chun Joaquim Manuel da Costa Pereira Sergey Efetov Ricardo Escalante Qing-Si He Jun-Hong Hu Cuneyt Kayaalp Seon-Hahn Kim Jim S.Khan Li-Jen Kuo Atsushi Nishimura Fernanda Nogueira Junji Okuda Avanish Saklani Ali A.Shafik Ming-Yin Shen Jung-Tack Son Jun-Min Song Dong-Hui Sun Keisuke Uehara Gui-Yu Wang Ye Wei Zhi-Guo Xiong Hong-Liang Yao Gang Yu Shao-Jun Yu Hai-Tao Zhou Suk-Hwan Lee Petr V.Tsarkov Chuan-Gang Fu Xi-Shan Wang The International Alliance of NOSES | 2019 | Gastroenterology Report2019，7，1： | 3 |
| 6 | Potential of Helper-Dependent Adenoviral Vectors in Modulating Airway Innate Immunity显示文摘Innate immune responses form the first line of defense against foreign insults and recently significant advances have been made in our understanding of the initiation of innate immune response along with its ability to modulate inflammation. In airway diseases such as asthma, COPD and cystic fibrosis, over reacting of the airway innate immune responses leads to cytokine imbalance and airway remodeling or damage. Helper-dependent adenoviral vectors have the potential to deliver genes to modulate airway innate immune responses and have many advantages over its predecessors. However, there still are a few limitations that need to be addressed prior to their use in clinical applications. | Rahul Kushwah Huibi Cao Jim Hu | 2007 | Cellular & Molecular Immunology2007，4，2： | 2 |
| 7 | Effect of CC Chemokine Receptor 2 CCR2 Blockade on Serum C-Reactive Protein in Individuals at Atherosclerotic Risk and With a Single Nucleotide Polymorphism of the Monocyte Chemoattractant Protein-1 Promoter Region显示文摘 | Jim Gilbert Julie Lekstrom-Himes Debra Donaldson Yih Lee Mingxiu Hu Jing Xu Tim Wyant Michael Davidson | 2011 | The American Journal of Cardiology2011，，6： | 2 |
| 8 | Technique and Outcomes of Robot-assisted Retroperitoneoscopic Partial Nephrectomy: A Multicenter Study显示文摘 | Jim C. Hu Eric Treat Christopher P. Filson Ian McLaren Siwei Xiong Sevan Stepanian Khaled S. Hafez Alon Z. Weizer James Porter | 2014 | European Urology2014，，： | 2 |
| 9 | Lung gene therapydHow to capture illumination from the light already present in the tunnel显示文摘Gene therapy has been considered as the most ideal medical intervention for genetic diseases because it is intended to target the cause of diseases instead of disease symptoms.Availability of techniques for identification of genetic mutations and for in vitro manipulation of genes makes it practical and attractive.After the initial hype in 1990s and later disappointments in clinical trials formore than a decade,light has finally come into the tunnel in recent years,especially in the field of eye gene therapy where it has taken big strides.Clinical trials in gene therapy for retinal degenerative diseases such as Leber’s congenital amaurosis(LCA)and choroideremia demonstrated clear therapeutic efficacies without apparent side effects.Although these successful examples are still rare and sporadic in the field,they provide the proof of concept for harnessing the power of gene therapy to treat genetic diseases and to modernize our medication.In addition,those success stories illuminate the path for the development of gene therapy treating other genetic diseases.Because of the differences in target organs and cells,distinct barriers to gene delivery exist in gene therapy for each genetic disease.It is not feasible for authors to review the current development in the entire field.Thus,in this article,we will focus onwhatwe can learn from the current success in gene therapy for retinal degenerative diseases to speed up the gene therapy development for lung diseases,such as cystic fibrosis. | Emily Xia Manjunatha Ankathatti Munegowda Huibi Cao Jim Hu | 2014 | Genes & Diseases2014，1，1： | 2 |
| 10 | Incomplete Inhibition of Thromboxane Biosynthesis by Acetylsalicylic Acid: Determinants and Effect on Cardiovascular Risk显示文摘 | John W. Eikelboom Graeme J. Hankey Jim Thom Deepak L. Bhatt P Gabriel Steg Gilles Montalescot S Claiborne Johnston Steven R. Steinhubl Koon-Hou Mak J Donald Easton Christian Hamm Tingfei Hu Keith A.A. Fox Eric J. Topol | 2008 | Circulation2008，，17： | 1 |
| 11 | Challenges to China's transition to a low carbon electricity system显示文摘 | Fredrich Kahrl Jim Williams Ding Jianhua Hu Junfeng | | 0，，39： | 1 |
| 12 | Anatomic Bladder Neck Preservation During Robotic-Assisted Laparoscopic Radical Prostatectomy: Description of Technique and Outcomes显示文摘 | Marcos P. Freire Aaron C. Weinberg Yin Lei Jane R. Soukup Stuart R. Lipsitz Sandip M. Prasad Fernando Korkes Tiffany Lin Jim C. Hu | 2009 | European Urology2009，，： | 1 |
| 13 | Strategies for Data Center Temperature Control during a Cooling System Outage显示文摘 | Mi Lin Shuangquan Shao Simon Zhang Jim VanGilder Victor Avelar Xiaopeng Hu | 2013 | Energy & Buildings2013，，： | 1 |
| 14 | Use, Costs and Comparative Effectiveness of Robotic Assisted, Laparoscopic and Open Urological Surgery显示文摘 | Hua-yin Yu Nathanael D. Hevelone Stuart R. Lipsitz Keith J. Kowalczyk Jim C. Hu | 2012 | The Journal of Urology2012，，4： | 1 |
| 15 | Laparoscopie versus open myomectomy- a meta- analysic of randomized controlled trials显示文摘 | Jim C Hu Y Chen XC | 2009 | Eur J Obstet Gynecol Reorod Biol2009，145，1： | 1 |
| 16 | ZNF403,一个新的细胞周期调节因子的功能研究(英文)显示文摘ZNF403和LCRG1是人类基因ZNF403的2个不同转录剪切本.以往的研究表明LCRG1在喉癌细胞株Hep-2中具有抑瘤特性.本研究旨在探明ZNF403和LCRG1不同剪切本之间的关系以及在肿瘤细胞中对ZNF403的功能进行研究.首先,采用实时荧光定量PCR对这2个转录本的相对表达水平进行分析,结果表明,ZNF403表达水平在不同细胞株中明显高于LCRG1(>10倍),为该基因的主要转录表达产物.随后分别采用MTT细胞生长分析法和裸鼠体内成瘤实验在体外和体内对ZNF403的功能进行分析,结果显示ZNF403的基因沉默可以同时在体内和体外抑制喉癌细胞Hep-2细胞的生长.为了探明其作用机制,本研究还采用细胞信息学、流式细胞周期分析术和高通量PCR点阵分析方法进一步分析,结果表明,ZNF403的基因沉默可显著抑制细胞DNA的复制并延缓细胞周期进入到有丝分裂期.同时发现ZNF403可调节一系列的细胞周期调节蛋白如MCM2、p21、ATM、MRE11A等.综上研究提示ZNF403为一新的细胞周期调节因子,其功能的缺失与肿瘤发生发展密切相关. | 关瑞 侯德富 饶翔 关勇军 欧阳咏梅 余艳辉 Jim HU 陈主初 | 2013 | 生物化学与生物物理进展2013，40，2： | 1 |
| 17 | Authentication of experimental materials:A remedy for the reproducibility crisis?显示文摘Reproducibility has always been a serious challenge when medical researchers in both academia and industry have tried to build upon previously published discoveries.Blindly chasing faulty results has incurred a huge waste of human and monetary resources.The damage to the progress of scientific discoveries,as well as their application to human well-being,cannot be overestimated.According to two reports by Bayer and Amgen published in 2011 and 2012,64e89%of the socalled“landmark”results could not be reproduced in their pre-clinical validation experiments.1,2 One plausible explanation for this out of proportion irreproducibility is related to the intricacy of the scientific experiments,including the sourcing of reagent antibodies and cell lines,which are major sources of variations.To make validation meaningful,the study materials used in the original studies need to be authenticated so that variations due to the faulty materials can be prevented during follow-up studies.However,the technical complexity and the costs of authentication often discourage this practice in research laboratories. | Fei Li Jim Hu Keping Xie Tong-Chuan He | 2015 | Genes & Diseases2015，2，4： | 1 |
| 18 | Children's respiratory morbidity prevalence in relation to air pollution in four Chinese cities显示文摘 | Wei Hu Fusheng Wei | 2002 | Environmental Health Perspectives2002，11，9： | 1 |
| 19 | Use, Costs and Comparative Effectiveness of Robotic Assisted, Laparoscopic and Open Urological Surgery显示文摘 | Hua-yin Yu Nathanael D. Hevelone Stuart R. Lipsitz Keith J. Kowalczyk Jim C. Hu | 2012 | The Journal of Urology2012，，4： | 1 |
| 20 | Highly efficient retinal gene delivery with helper-dependent adenoviral vectors显示文摘There have been significant advancements in the field of retinal gene therapy in the past several years.In particular,therapeutic efficacy has been achieved in three separate human clinical trials conducted to assess the ability of adeno-associated viruses(AAV)to treat of a type of Leber’s congenital amaurosis caused by RPE65 mutations.However,despite the success of retinal gene therapy with AAV,challenges remain for delivering large therapeutic genes or genes requiring long DNA regulatory elements for controlling their expression.For example,Stargardt’s disease,a form of juvenile macular degeneration,is caused by defects in ABCA4,a gene that is too large to be packaged in AAV.Therefore,we investigated the ability of helper dependent adenovirus(HD-Ad)to deliver genes to the retina as it has a much larger transgene capacity.Using an EGFP reporter,our results showed that HD-Ad can transduce the entire retinal epithelium of a mouse using a dose of only 1105 infectious units and maintain transgene expression for at least 4 months.The results demonstrate that HD-Ad has the potential to be an effective vector for the gene therapy of the retina. | Simon Lam Huibi Cao Jing Wu Rongqi Duan Jim Hu | 2014 | Genes & Diseases2014，1，2： | 1 |
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