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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Advances in research of the mid-deep South China Sea circulation显示文摘The South China Sea(SCS)is a large marginal sea connecting the Indian and Pacific oceans.Under the factors of monsoons,strait transport,and varied bathymetry,the SCS presents a three-layer structure and strong diapycnal mixing which is far greater than that in the open ocean.Theoretical analysis and observations reveal that internal tides,internal solitary waves,and strong winds are the sources of the strong mixing in the northern SCS.A major consequence of the strong mixing is an active mid-deep circulation system.This system promotes exchange of water between the SCS and adjacent oceans,and also regulates the upper layer of wind-driven circulation,making the 3 dimensional SCS circulation clearly different from that in other tropical and subtropical marginal seas.The mass transport capacity of the mid-deep circulation has a substantial impact on marine sedimentation,the biogeochemical cycle,and other processes in the SCS.This paper summarizes the recent advances in middeep sea circulation dynamics of the SCS,and discusses the opportunities and challenges in this area. | Dongxiao WANG Qiang WANG Shuqun CAI Xiaodong SHANG Shiqiu PENG Yeqiang SHU Jingen XIAO Xiaohui XIE Zhiwei ZHANG Zhiqiang LIU Jian LAN Dake CHEN Huijie XUE Guihua WANG Jianping GAN Xinong XIE Rui ZHANG Hui CHEN Qingxuan YANG | 2019 | Science China Earth Sciences2019，62，12： | 8 |
| 3 | Comparison of typhoon-induced near-inertial oscillations in shear flow in the northern South China Sea显示文摘With moorings equipped with Acoustic Doppler Current Profilers(ADCP) in the northern South China Sea(SCS)in 2008 and 2009, we observed three near-inertial oscillation(NIO) events coded 2008 a, 2009 a and 2009 b induced by passages of typhoons or tropical storms. This study compares characteristics of the three NIO events. Event2008 a was the strongest one among the three, and had the longest sustaining period(15 d), while events 2009 a and 2009 b sustained for only 4 and 8 d, respectively. The three events were distinguished by vertical energy distribution and phase propagation. As for the frequency shift of the NIO, event 2008 a had a peak frequency lower than the local Coriolis frequency(red-shift), while events 2009 a and 2009 b showed blue-shift. The behavior of individual NIO event is jointly decided by the typhoon disturbance and the background ocean condition.Especially the background flow plays an important role by effects of advection and modulation. The results in this study provide observational evidence of variational NIO response to background flow field. As indicated by the distribution of vorticity and effective Coriolis frequency derived from numerical modeling, the large amplitude and elongated sustaining period of event 2008 a were attributed to the waveguide effect of the background shear flow. This effect redistributed the NIO energy after the typhoon passage, absorbed incident waves and trapped energy in the area of the negative vorticity. While the background flow during events 2009 a and 2009 b did not have such effects due to the near-zero vorticity in the mooring area. | SUN Zhenyu HU Jianyu ZHENG Quanan GAN Jianping | 2015 | Acta Oceanologica Sinica2015，34，11： | 3 |
| 4 | Efficacy of Rebixiao Chinese herbal tablets and Chinese formula granules in acute gout arthritis patients:a randomized,multicenter,double-blind,controlled trial显示文摘OBJECTIVE:To evaluate the clinical efficacy and safety of Rebixiao(RBX)Chinese herbal tablets(CHT)and Chinese formula granules(CFG)in the treatment of acute gout arthritis(AGA).METHODS:This randomized,multicenter,double-blind,controlled trial included 165 AGA patients with the damp-heat symptom pattern who were randomly divided into an RBX CHT group and an RBX CFG group and treated for 7 d at three centers.The total effective rates of the joint symptom score,Traditional Chinese Medicine(TCM)symptoms score,erythrocyte sedimentation rate(ESR),and C-reactive protein(CRP)were used to evaluate the clinical efficacy.Safety assessments were also performed.RESULTS:Of the 165 enrolled patients,147 completed the clinical observation.There was no difference in baseline between the two groups.The total effective rates of the joint symptom score were 94.36%and 97.36%,and the total effective rates of the TCM symptoms score were 95.77%and 97.36%in the CFG group and CHT group,respectively.No statistical difference was found between the two groups(P>0.05).Additionally,ESR and CRP were similar in both groups(P>0.05).Furthermore,treatment efficacy regarding TCM and joint symptoms,the ESR,and CRP were consistent within each center and among the different centers(P>0.05).In addition,the incidence of adverse events was 4.22%and 2.63%in the CFG group and CHT group,respectively,and no difference was observed between the two groups(P>0.05).CONCLUSION:RBX CFG and CHT have significant and similar efficacy in the treatment of AGA,and CFG did not increase adverse side effects. | Wang Shasha Zhang Ying Dai Min Yang Min Leng Wenfei Peng Xiufeng Li Guojun Yang Jinbing Gan Jianping Zhu Fenglin Li Yanping Wu Bin | 2020 | Journal of Traditional Chinese Medicine2020，40，4： | 3 |
| 5 | Discriminating against injectable fat emulsions with similar formulation based on water quenching fluorescent probe显示文摘The discrimination against nutritional fat emulsion injections was considered by imaging tools,which aims to elucidate the in vivo behaviors of nanoemulsions.In this study,20%nutritional fat emulsion injections were selected from different company including original and generic products.Meanwhile,a water quenching fluorescent probe(P2)was used to label them by an incubation method.The fluorescent intensity analysis of blood-borne fluorescence reveals rapid clearance of nanoemulsion in all groups,which shows'L'-type blood kinetic profiles.However,these kinetic parameters do not have significant difference.Following intravenous administration,the nanoemulsions in all groups concomitantly accumulated in organs of reticulo-endothelial system(RES),such as liver and spleen,and were cleared from body circulation mostly after 12 h.AUC(0-t)of organs from different groups showed dissimilar results in some organs.These intuitional results are of significance in understa nding the in vivo behaviors of nanoemulsions,which can provide a new way to discriminate against nutritional fat emulsions. | Jinlong Yang Zirong Dong Wenjuan Liu Haisheng He Wufa Fan Yi Lu Wei Wu Li Gan Jianping Qi | 2020 | Chinese Chemical Letters2020，31，3： | 1 |
| 6 | Eddy heat and salt transports in the South China Sea and their seasonal modulations显示文摘 | CHEN Gengxin GAN Jianping XIE Qiang | 2012 | Journal of Geophysical Research2012，117，5： | 1 |
| 7 | Systematic dissection of disease resistance to southern corn rust by bulked-segregant and transcriptome analysis显示文摘Southern corn rust(SCR) is a destructive maize disease caused by Puccinia polysora Underw. To investigate the mechanism of SCR resistance in maize, a highly resistant inbred line, L119 A, and a highly susceptible line, Lx9801, were subjected to gene mapping and transcriptome analysis. Bulked-segregant analysis coupled with whole-genome sequencing revealed several quantitative trait loci(QTL) on chromosomes 1, 6, 8, and 10. A set of 25 genes, including two coiled-coil nucleotide-binding site leucine-rich repeat(CC-NBS-LRR) genes, were identified as candidate genes for a major-effect QTL on chromosome 10. To investigate the mechanism of SCR resistance in L119 A, RNA-seq of P. polysorainoculated and non-inoculated plants of L119 A and Lx9801 was performed. Unexpectedly, the number of differentially expressed genes in inoculated versus non-inoculated L119 A plants was about 10 times that of Lx9801, with only 29 common genes identified in both lines, suggesting extensive gene expression changes in the highly resistant but not in the susceptible line. Based on the transcriptome analysis, one of the CC-NBS-LRR candidate genes was confirmed to be upregulated in L119 A relative to Lx9801 independently of P. polysora inoculation. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses indicated that transcription factors, as well as genes involved in defense responses and metabolic processes, were dominantly enriched, with the phenylpropanoid biosynthesis pathway most specifically activated. Consistently, accumulation of phenylpropanoid-derived lignin, especially S lignin, was drastically increased in L119 A after P. polysora inoculation, but remained unchanged in Lx9801, suggesting a critical role of lignin in SCR resistance. A regulatory network of defense activation and metabolic change in SCR-resistant maize upon P. polysora infection is described. | Xiaohuan Mu Zhuangzhuang Dai Zhanyong Guo Hui Zhang Jianping Yang Xinke Gan Jiankun Li Zonghua Liu Jihua Tang Mingyue Gou | 2022 | The Crop Journal2022，10，2： | 1 |
| 8 | Numerical study of the tide and tidal dynamics in the South China Sea显示文摘 | Zu Tingting Gan Jianping Erofeeva Svetlana Y | 2008 | Deep-Sea Research2008，55，： | 1 |
| 9 | Observed three-dimensional structure of a cold eddy in thesouthwestern South China Sea显示文摘 | HU JIANYU GAN JIANPING SUN ZHENYU 2011 | 1978 | Journal of GeophysicalResearch: Oceans (1978-2012)1978，116，05： | 1 |
| 10 | Modeling seasonal and diurnal pCO2 variations in thenorthern South China Sea显示文摘 | LU ZHONGMING GAN JIANPING DAI MINHAN 2012 | 2012 | Journal of Marine Systems2012，92，1： | 1 |
| 11 | Interaction of a river plume with coastal upwelling in thenortheastern South China Sea 显示文摘 | GAN JIANPING LI LI WANG DONGXIAO 2009 | 2009 | Continental Shelf Research2009，29，4： | 1 |
| 12 | Eddy heat and salt transports in the South China Sea and their seasonal modula-tions显示文摘 | Chen Gengxin Gan Jianping Xie Qiang | | 0，，： | 1 |
| 13 | Interaction of a river plume with coastal upwelling in the northeastern South China Sea 显示文摘 | GAN JIANPING LI LI WANG DONGXIAO | 2009 | Continental Shelf Research2009，29，4： | 1 |
| 14 | Observed three-di- mensional structure of a cold eddy in the southwestern South Chi- na Sea显示文摘 | Hu Jianyu Gan Jianping Sun Zhenyu | 2011 | J Geophys Res2011，116，05016： | 1 |
| 15 | Interaction of a river plume with coastal upwelling in the northeastern South China Sea显示文摘 | GAN JIANPING LI LI WANG DONGXIAO | 2009 | Cont Shelf Res2009，29，4： | 1 |
| 16 | Numerical study of the tide and tidal dynamics in theSouth China Sea显示文摘 | ZU TINGTING GAN JIANPING EROFEEVA S Y | 2008 | Deep-Sea Res:PartⅠ2008，55，13： | 1 |
| 17 | Timing and Single-pulse Study of Pulsar J1909+0122 Discovered by CRAFTS显示文摘We report the discovery of PSR J1909+0122 by the Five-hundred-meter Aperture Spherical Radio Telescope(FAST)as part of the Commensal Radio Astronomy FAST Survey.PSR J1909+0122 has a spin period of 1.257 s and a dispersion measure of 186.2 pc cm^(-3).The averaged pulse profile shows two distinct components.We performed a single-pulse study based on a one-hour observation at 1.25 GHz on 2021 August 23.We used a threshold of 5σ_(ep) to measure the nulling fraction(NF)as 63%±1.5%.The longitude-resolved fluctuation spectra and fast Fourier transform spectra of the binary sequences revealed the quasi-periodicity of nulling with a period of 30 rotation periods.We examined the reliability of the periodicity by comparing it to random noise injection.The NF,E,and modulation periodicity P_(M) of PSR J1909+0122 were compared with other periodic nulling pulsars,showing that the source of J1909+0122 has the second largest NF in the population.Long-term timing observations over six months were used to derive the phase-connected ephemeris of this pulsar.The measured P and P values disfavor dipolar geometry for polar gap models,and the prediction for a space-charge-limited flow model in the case of inverse Compton scattering is only just above the death line.In this work,PSR J1909+0122 has revealed possible correlations between nulling behavior and pulsar properties,which will help to shed light on the pulsar emission mechanism and its temporal evolution in future observations. | Yutong Chen Pei Wang Di Li Erbil Gügercinoğlu Rushuang Zhao Lingqi Meng Jianping Yuan Jiarui Niu Weiwei Zhu Yi Feng Chenchen Miao Chenhui Niu Qingdong Wu Na Wang Shen Wang Xiaoyao Xie Mengyao Xue Jumei Yao Mao Yuan Shanping You Xuhong Yu Youling Yue Jie Zhang Junshuo Zhang Lei Zhang Yabiao Wang Zhenye Gan Yuxi Li Zhongyi Sun Chengjie Wang | 2023 | Research in Astronomy and Astrophysics2023，23，8： | 0 |
| 18 | Dual phosphorylation of Sin1 at T86 and T398 negatively regulates mTORC2 complex integrity and activity显示文摘 | Pengda Liu Jianping Guo Wenjian Gan Wenyi Wei | 2014 | Protein & Cell2014，5，3： | 0 |
| 19 | Construction of a Microsatellite-enriched Library of Rhododendron simsii Planch.显示文摘In this study,an( AG) n microsatellite-enriched library of Rhododendron simsii Planch. was constructed with FIASCO( fast Isolation by AFLP sequences containing repeats) method. Among 356 positive colonies verified by colony PCR of the( AG) n microsatellite-enriched library,233 colonies were selected for further sequencing. According to the results,202 colonies containing microsatellites were obtained,accounting for 86. 7%. Discarding sequences with miscellaneous peaks,overlapping peaks and weak signals,39 sequences were screened finally for subsequent primer exploitation,including 22 perfect type SSR loci,5 imperfect type SSR loci and 12 compound type SSR loci. The FIASCO method is efficient and feasible for constructing microsatellite-enriched libraries of Rhododendron spp.,which laid a solid foundation for isolation of microsatellite loci and investigation of genetic diversity and genetic structure of Rhododendron spp. | Shuzhen WANG Weibin JIN Jun XIANG Yongliang ZHENG Yuanping FANG Jianping GAN Hua CHENG Hang DU | 2015 | Agricultural Biotechnology2015，4，5： | 0 |
| 20 | Retrospective Analysis of the Duration of Hospitalization of Imported Patients Infected with SARS-CoV-2 in Guangzhou, 2021显示文摘The pandemic of coronavirus disease 2019 is“not over,”in fact,the“dynamic clearing”policy for SARS-CoV-2 control and prevention in China has been firmly enforced.This study aimed to analyze the clinical symptoms and dynamic viral RNA changes in 2021 at Guangzhou Eighth People’s Hospital.This study showed that 31.4%of the patients(695/2212)tested negative for viral RNA from admission to the final release from quarantine.Of all negative cases,86.5%(601/695)remained in the hospital for no more than 5 days and were asymptomatic or mild.Among the remaining 402 patients who stayed for no more than 5 days,76.4%(307/402)were viral RNA retest positive during the isolation stage.However,96.4%of the peak viral RNA(296/307)was over Ct=33 cycles during the isolation stage. | Zhiwei Xie Guofang Tang Lu Li Jingrong Shi Qingxin Gan Xiaowen Zheng Jingyan Tang Huimin Zeng Chuyu Zhang Sisi Cheni Jianping Cui Zishi Lin Lihua Lin Youxia Li Jinxin Liu Feng Li Fengyu Hui Xiaoping Tang Xilong Deng | 2023 | Infectious Diseases & Immunity2023，3，1： | 0 |
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