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| 1 | High-throughput screening of mouse gene knockouts identifies established and novel skeletal phenotypes显示文摘Screening gene function in vivo is a powerful approach to discover novel drug targets. We present high-throughput screening(HTS) data for 3 762 distinct global gene knockout(KO) mouse lines with viable adult homozygous mice generated using either gene-trap or homologous recombination technologies. Bone mass was determined from DEXA scans of male and female mice at 14 weeks of age and by microCT analyses of bones from male mice at 16 weeks of age. Wild-type(WT) cagemates/littermates were examined for each gene KO. Lethality was observed in an additional 850 KO lines. Since primary HTS are susceptible to false positive findings, additional cohorts of mice from KO lines with intriguing HTS bone data were examined. Aging,ovariectomy, histomorphometry and bone strength studies were performed and possible non-skeletal phenotypes were explored. Together, these screens identified multiple genes affecting bone mass: 23 previously reported genes(Calcr, Cebpb, Crtap, Dcstamp, Dkk1, Duoxa2, Enpp1, Fgf23, Kiss1/Kiss1 r, Kl(Klotho),Lrp5, Mstn, Neo1, Npr2, Ostm1, Postn, Sfrp4, Slc30a5, Slc39a13, Sost, Sumf1, Src, Wnt10b), five novel genes extensively characterized(Cldn18, Fam20 c, Lrrk1, Sgpl1, Wnt16), five novel genes with preliminary characterization(Agpat2, Rassf5, Slc10a7, Slc26a7, Slc30a10) and three novel undisclosed genes coding for potential osteoporosis drug targets. | Robert Brommage Jeff Liu Gwenn M Hansen Laura L Kirkpatrick David G Potter Arthur T Ss Brian Zambrowicz David R Powell Peter Vogel | 2014 | Bone Research2014，2，3： | 7 |
| 2 | The Glutamatergic Postrhinal Cortex–Ventrolateral Orbitofrontal Cortex Pathway Regulates Spatial Memory Retrieval显示文摘A deficit in spatial memory has been taken as an early predictor of Alzheimer’s disease(AD) or mild cognitive impairment(MCI). The uncinate fasciculus(UF) is a long-range white-matter tract that connects the anterior temporal lobe with the orbitofrontal cortex(OFC)in primates. Previous studies have shown that the UF impairment associated with spatial memory deficits may be an important pathological change in aging and AD, but its exact role in spatial memory is not well understood. The pathway arising from the postrhinal cortex(POR) and projecting to the ventrolateral orbitofrontal cortex(vlOFC)performs most of the functions of the UF in rodents.Although the literature suggests an association between spatial memory and the regions connected by the POR–vlOFC pathway, the function of the pathway in spatialmemory is relatively unknown. To further illuminate the function of the UF in spatial memory, we dissected the POR–vlOFC pathway in mice. We determined that the POR–vlOFC pathway is a glutamatergic structure, and that glutamatergic neurons in the POR regulate spatial memory retrieval. We also demonstrated that the POR–vlOFC pathway specifically transmits spatial information to participate in memory retrieval. These findings provide a deeper understanding of UF function and dysfunction related to disorders of memory, as in MCI and AD. | Xinyang Qi Zhanhong Jeff Du Lin Zhu Xuemei Liu Hua Xu Zheng Zhou Cheng Zhong Shijiang Li Liping Wang Zhijun Zhang | 2019 | Neuroscience Bulletin2019，35，3： | 7 |
| 3 | MicroRNAs in nasopharyngeal carcinoma显示文摘MicroRNAs(miRNAs) provide insight into both the biology and clinical behavior of many human cancers, including nasopharyngeal carcinoma(NPC). The dysregulation of miRNAs in NPC results in a variety of tumor-promoting effects. Furthermore, several miRNAs are prognostic markers for NPC. In addition to cellular miRNAs, NPC samples also often contain miRNAs encoded by Epstein-Barr virus, and these miRNAs may impact NPC biology by targeting both cellular and viral genes. Given their numerous putative roles in NPC development and progression, a thorough understanding of the impact of miRNA dysregulation in NPC is expected to shed light on useful biomarkers and therapeutic targets for the clinical management of this disease. In this review, we describe the efforts to date to identify and characterize such miRNAs in the context of NPC. | Jeff P.Bruce Fei-Fei Liu | 2014 | Chinese Journal of Cancer2014，33，11： | 6 |
| 4 | Optical observations of LIGO source GW 170817 by the Antarctic Survey Telescopes at Dome A,Antarctica显示文摘The LIGO detection of gravitational waves(GW) from merging black holes in 2015 marked the beginning of a new era in observational astronomy. The detection of an electromagnetic signal from a GW source is the critical next step to explore in detail the physics involved. The Antarctic Survey Telescopes(AST3),located at Dome A, Antarctica, is uniquely situated for rapid response time-domain astronomy with its continuous night-time coverage during the austral winter. We report optical observations of the GW source(GW 170817) in the nearby galaxy NGC 4993 using AST3. The data show a rapidly fading transient at around 1 day after the GW trigger, with the i-band magnitude declining from 17:23 ± 0:13 magnitude to 17:72 ± 0:09 magnitude in ~1:8 h. The brightness and time evolution of the optical transient associated with GW 170817 are broadly consistent with the predictions of models involving merging binary neutron stars. We infer from our data that the merging process ejected about ~10^(-2) solar mass of radioactive material at a speed of up to 30% the speed of light. | lei hu xuefeng wu igor andreoni michael c.b.ashley jeff cooke xiangqun cui fujia du zigao dai bozhong gu yi hu haiping lu xiaoyan li zhengyang li ensi liang liangduan liu bin ma zhaohui shang tianrui sun n.b.suntzeff charling tao syed a.uddin lifan wang xiaofeng wang haikun wen di xiao jin xu ji yang shihai yang xiangyan yuan hongyan zhou hui zhang jilin zhou zonghong zhu | 2017 | Science Bulletin2017，62，21： | 6 |
| 5 | Development and optimization of thermal contrast amplification lateral flow immunoassays for ultrasensitive HIV p24 protein detection显示文摘Detection of human immunodeficiency virus(HIV)p24 protein at a single pg/ml concentration in point-of-care(POC)settings is important because it can facilitate acute HIV infection diagnosis with a detection sensitivity approaching that of laboratory-based assays.However,the limit of detection(LOD)of lateral flow immunoassays(LFAs),the most prominent POC diagnostic platform,falls short of that of laboratory protein detection methods such as enzyme-linked immunosorbent assay(ELISA).Here,we report the development and optimization of a thermal contrast amplification(TCA)LFA that will allow ultrasensitive detection of 8 pg/ml p24 protein spiked into human serum at POC,approaching the LOD of a laboratory test.To achieve this aim,we pursued several innovations as follows:(a)defining a new quantitative figure of merit for LFA design based on the specific to nonspecific binding ratio(BR);(b)using different sizes and shapes of gold nanoparticles(GNPs)in the systematic optimization of TCA LFA designs;and(c)exploring new laser wavelengths and power regimes for TCA LFA designs.First,we optimized the blocking buffer for the membrane and running buffer by quantitatively measuring the BR using a TCA reader.The TCA reader interprets the thermal signal(i.e.,temperature)of GNPs within the membrane when irradiated by a laser at the plasmon resonance wavelength of the particle.This process results in higher detection and quantitation of GNPs than in traditional visual detection(i.e.,color intensity).Further,we investigated the effect of laser power(30,100,200 mW),GNP size and shape(30 and 100 nm gold spheres,150 nm gold-silica shells),and laser wavelength(532,800 nm).Applying these innovations to a new TCA LFA design,we demonstrated that 100nm spheres with a 100 mW 532 nm laser provided the best performance(i.e.,LOD=8pg/ml).This LOD is significantly better than that of the current colorimetric LFA and is in the range of the laboratory-based p24 ELISA.In summary,this TCA LFA for p24 protein shows promise for detecting acute HIV infection in POC settings. | Li Zhan Timothy Granade Yilin Liu Xierong Wei Ae Youngpairoj Vickie Sullivan Jeff Johnson John Bischof | 2020 | Microsystems & Nanoengineering2020，6，1： | 3 |
| 6 | NOTUM inhibition increases endocortical bone formation and bone strength显示文摘The disability,mortality and costs caused by non-vertebral osteoporotic fractures are enormous.Existing osteoporosis therapies are highly effective at reducing vertebral but not non-vertebral fractures.Cortical bone is a major determinant of non-vertebral bone strength.To identify novel osteoporosis drug targets,we phenotyped cortical bone of 3 366 viable mouse strains with global knockouts of druggable genes.Cortical bone thickness was substantially elevated in Notum?/?mice.NOTUM is a secreted WNT lipase and we observed high NOTUM expression in cortical bone and osteoblasts but not osteoclasts.Three orally active small molecules and a neutralizing antibody inhibiting NOTUM lipase activity were developed.They increased cortical bone thickness and strength at multiple skeletal sites in both gonadal intact and ovariectomized rodents by stimulating endocortical bone formation.Thus,inhibition of NOTUM activity is a potential novel anabolic therapy for strengthening cortical bone and preventing non-vertebral fractures. | Robert Brommage Jeff Liu Peter Voge Faika Mseeh Andrea Y.Thompson David G.Potter Melanie K.Shadoan Gwenn M.Hansen Sabrina Jeter-Jones Jie Cui Dawn Bright Jennifer P.Bardenhagen Deon D.Doree Sofia Moverare-Skrtic Karin H.Nilsson Petra Henning Ulf H.Lerner Claes Ohlsson Arthur T.Sands James E.Tarver David R.Powell Brian Zambrowicz Qingyun Liu | 2019 | Bone Research2019，7，1： | 2 |
| 7 | Wisconsin Ginseng (Panax quinquefolius) to Improve Cancer-Related Fatigue: A Randomized, Double-Blind Trial, N07C2显示文摘 | Debra L. Barton Heshan Liu Shaker R. Dakhil Breanna Linquist Jeff A. Sloan Craig R. Nichols Travis W. McGinn Philip J. Stella Grant R. Seeger Amit Sood Charles L. Loprinzi | 2013 | JNCI Journal of the National Cancer Institute2013，，16： | 1 |
| 8 | Generating test data from state-based specifications显示文摘 | Jeff Offutt Liu Shaoying Aynur Abdurazik | 2003 | Journal of Software Testing Verification and Reliability2003，13，1： | 1 |
| 9 | Quality of life over time in women diagnosed with ductal carcinoma in situ,early-stage invasive breast cancer,and age-matched controls显示文摘 | Jeffe DB Pérez M Liu Y | | 0，，01： | 1 |
| 10 | Generating Test Data From State-based Specifications显示文摘 | Jeff Offutt Shaoying Liu Aynur Abdurazik | 2003 | The Journal of Software Testing Verification and Reliability2003，13，1： | 1 |
| 11 | Generating Test Data From State-based Specifications显示文摘 | Jeff Offutt Shaoying Liu Aynur Abdurazik | 2005 | The Journal of Software Testing Verification And Reliability2005，13，1： | 1 |
| 12 | The effect of a submarine canyon on the river sediment dispersal and inner shelf sediment movements in southern Taiwan显示文摘 | LIU T James LIU Kuen-jang HUANG Jeff C | 2002 | Marine Geology2002，181，4： | 1 |
| 13 | Spatiotemporal Regulation of Epithelial-Mesenchymal Transition Is Essential for Squamous Cell Carcinoma Metastasis显示文摘 | Jeff H. Tsai Joana Liu Donaher Danielle A. Murphy Sandra Chau Jing Yang | 2012 | Cancer Cell2012，，6： | 1 |
| 14 | 西加拿大沉积盆地中的水力压裂和地震活动显示文摘绝大多数非常规油气资源的开发有赖于向地下注入大量的液体,这可能使附近断层活动从而诱发地震。在过去5年里,加速的油田注水已经导致北美部分地区的地震频率急剧增加。在美国中部,大多数诱发地震起因于与油气开采相伴生的废水的排放。形成对比的是,在加拿大西部,大多数最近的诱发地震在时间和空间上与水力压裂高度相关,在水力压裂过程中,为诱发局部岩石破裂,在钻井结束的过程中,高压液体被注入。并且,与水力压裂相关的观测到的最大地震震级似乎超越了预测的、经常引用的注入液体体积与最大期望震级之间的关系。这些发现对评估诱发地震危害具有深远的意义。 | Gail M.Atkinso David W.Eaton Hadi Ghofrani Dan Walker Burns Cheadle Ryan Schultz Robert Shcherbakov Kristy Tiampo Jeff Gu Rebecca M.Harrington Yajing Liu Mirko van der Baan Honn Kao 张汨 | 2018 | 国际地震动态2018，0，10： | 1 |
| 15 | Targeted disruption of leucine‐rich repeat kinase 1 but not leucine‐rich repeat kinase 2 in mice causes severe osteopetrosis显示文摘 | Weirong Xing Jeff Liu Shaohong Cheng Peter Vogel Subburaman Mohan Robert Brommage | 2013 | J Bone Miner Res2013，，9： | 1 |
| 16 | Randomized, placebo-controlled trial of sertraline and contingency management for the treatment of methamphetamine dependence 显示文摘 | Shoptaw S Huber A Peck J Yang X Liu J Jeff Dang | 2006 | Drug Alcohol Depend2006，85，1： | 1 |
| 17 | Gener-ating test data from SQFL specification显示文摘 | Offutt Jeff LIU Shaoying Abdurazik Aynur | 2003 | The Jour-nal of Software Testing* Verification and Reliability2003，13，1： | 1 |
| 18 | Tumor Necrosis Factor-α Mediates Cardiac Remodeling and Ventricular Dysfunction After Pressure Overload State显示文摘 | Mei Sun Manyin Chen Fayez Dawood Urszula Zurawska Jeff Y. Li Thomas Parker Zamaneh Kassiri Lorrie A. Kirshenbaum Malcolm Arnold Rama Khokha Peter P. Liu | 2007 | Circulation2007，，11： | 1 |
| 19 | Quantitative analysis of micro structural and conductivity evolution of Ni-YSZ anodes during thermal cycling based on nano-computed tomography显示文摘 | Yong Guan Yunhui Gong Wenjie Li Jeff Gelb Lei Zhang Gang Liu Xiaobo Zhang Xiangxia Song Changrong Xia Ying Xiong Haiqian Wang Ziyu Wu Yangchao Tian | 2011 | Journal of Power Sources2011，，24： | 1 |
| 20 | RB1 and p53 at the crossroad of EMT and triple-negative breast cancer显示文摘 | Zhe Jiang Robert Jones Jeff C. Liu Tao Deng Tyler Robinson Philip E.D. Chung Sharon Wang Jason I. Herschkowitz Sean E. Egan Charles M. Perou Eldad Zacksenhaus | 2011 | Cell Cycle2011，，10： | 1 |
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